LOCUS       NZ_AP025858             2511 bp    DNA     circular CON 21-APR-2024
DEFINITION  Enterobacter hormaechei strain FUJ80144 plasmid pFJMB80144_3,
            complete sequence.
ACCESSION   NZ_AP025858
VERSION     NZ_AP025858.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00490873
            Assembly: GCF_023735515.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Rapid phylogenetic analysis of Enterobacter hormaechei using open
            reading frame content patterns acquired by Oxford Nanopore
            sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2022) Contact:Masahiro Suzuki Fujita Health
            University, School of Medicine, Microbiology; Dengakugakubo 1-98,
            Kutsukake-cho, Toyoake, Aichi 470-1192, Japan URL
            :http://www.fujita-hu.ac.jp/~microb/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9b
            Genome Coverage                   :: 82x
            Sequencing Technology             :: MiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023735515.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 05:16:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,296
            CDSs (total)                      :: 5,181
            Genes (coding)                    :: 5,086
            CDSs (with protein)               :: 5,086
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 27 of 95
            Pseudo Genes (incomplete)         :: 72 of 95
            Pseudo Genes (internal stop)      :: 12 of 95
            Pseudo Genes (multiple problems)  :: 14 of 95
            Pseudo Genes (short protein)      :: 1 of 95
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP025858.1.
            Annotated by DFAST v.1.2.17 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="FUJ80144"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pFJMB80144_3"
                     /geo_loc_name="Japan:Aichi"
                     /lat_lon="35.07 N 137.00 E"
                     /collection_date="2019-06-24"
     gene            34..219
                     /locus_tag="NGJ60_RS26430"
     CDS             34..219
                     /locus_tag="NGJ60_RS26430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032105038.1"
                     /translation="MTRQESAALNMAKFIRSQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYTMLNAQTGEEDA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1380..1523
                     /locus_tag="NGJ60_RS26435"
                     /old_locus_tag="FJMB80144_51970"
     CDS             1380..1523
                     /locus_tag="NGJ60_RS26435"
                     /old_locus_tag="FJMB80144_51970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032610644.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHVKTPLAAATGKGYTRLLVQ
                     SS"
                     /name="hypothetical protein"
     gene            1951..2178
                     /locus_tag="NGJ60_RS26440"
                     /old_locus_tag="FJMB80144_51980"
     CDS             1951..2178
                     /locus_tag="NGJ60_RS26440"
                     /old_locus_tag="FJMB80144_51980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     Typing          complement(267..346)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.67e-34"
     Typing          985..1545
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.339"
                     /coverage="91.0"
                     /evalue="0.0"
ORIGIN
        1 gcaaaaatca tcaccagacg gggaaaatca ccagtgacca gacaggaatc cgccgccctc
       61 aacatggcca aatttatccg ctcacagaca ctcctcctcc tcgagcggct cgaacagatg
      121 gatctggatg aggctgccgg ctgctgtgag cacctgcacg atcaggccga agcactttac
      181 accatgctga acgcacagac aggcgaggaa gatgcgtgaa aatcggtgaa cgggtgcgca
      241 attcagtgcg cggccgtgag gcgatggcgg ggtgtcgggg cgcagccctg acctgggtat
      301 ttgtgatgcc ggcgcgtgcg cggtattaca aatacacatc ctgtcctgag tggttccccg
      361 ggaacaggca aaaaaaaacc ggggagctgc ccggaaggta agtcaagtta gattagatta
      421 ctttcactca tccataacag catcatgcaa cgacgaccat cgtccgtgac ggccgcctcg
      481 tttaagtatg gacagaaata cagaaaatgc tcaggacgaa atgtaatgaa tgcgaacgga
      541 ttcaagaaat tcgagcatga ccgtccttac ggccggttca gtttcagaca aaatctgccg
      601 gtatgcatcc agcatcatgg ctccggcatc cccccggcac gccggagcca gcccgaaaca
      661 acagacacaa acaggtgtcg ctcatcatca agagtcatca ggtctccgga agaaaaacca
      721 aacatggctt acctcctgtt aacggcaacg cagaagaaag gtttacaggg tagcgggcag
      781 gaaaataaaa aaattgcagt cagcccggaa cggctgctga accggtacag gcgggttccg
      841 ggcgcagcgc cggaccagtc actcagcgct agcggagtgt atactggcta aaatgtaaca
      901 ccgcagtaac gcatagcata actgctacat aagacaggcg cagaaaatga aagaaaacgc
      961 gtcaggagaa aatgtaatac aggatatatt ccgcttcctc gctcactcac tcgctgcgct
     1021 cggtcgttcg gctgcggcga gcggtacggg cttacgggtg gagcagaaat ttcctagggg
     1081 atgccagcta ggggatgcca ggaagatact tagcgaagaa ggcataaggc cgcgccgaag
     1141 ccgtttttcc ataggctccg cccccctgac gagcatcacg aaatctgacg ctcaaatcag
     1201 tggtggcgaa acccgatgcg gcttaaagat accaggcgtt tccccctgat ggctccctcg
     1261 tgctcccccg cattcctgcc tttcggttta ccggtgtcac tccgctgtta tggccgcggt
     1321 ttctcattcc acgcctgaca ctcggttccg ggaaggcagt tcgctccagg atggactgta
     1381 tgcacgaacc ccccgttcag tccgactgct gcgccttatc cggtaactat cgtcttgagt
     1441 ccaacccgga aagacacgta aaaacgccac tggcagcggc cactggtaag gggtatacaa
     1501 gattactggt gcagagttct tgaagttgac gcctgactgc ggctacacta gaaggacaaa
     1561 tttggtggca gcggtcttgt acaccctgtt accacggttc agaaacttgc cggtttgctg
     1621 aaccttcgaa aaaccacctt gccgggtggt tttttcgttt acagggcaag agattacgcg
     1681 cgaaaaaaaa ggatctcaag aagatccttt tataaattag acatccaacc tcacaaagag
     1741 gaatacttaa atcatcttgg tatatgtatc ggtcggatta catcagatat aagcccgcac
     1801 tgccccctcc ccgccagaca ccacacttta accataacac ctcgcaccta actcgatatg
     1861 cagcccacca tcaagcctct tttcacataa aaaatgacct atagttagat ggttagtgac
     1921 caaaactgat atggataaaa ggagctggct ttgaaaaaga tttttgtttc cgtgttcgca
     1981 gcggcagttg cgctttcagc cctcaccggc tgcacgcgca caagctatgc catacacacc
     2041 aatgacgggc gcactattgt cagcgacggc aaacctaaag aatcagactc aggcctgctt
     2101 ggctatacag acgcgaatgg tgtcaaacag cagatcaata aaaccgacgt taaagaagtc
     2161 tccgaaattc cgcactgagc atcttctccg aaaaaggccg ctaatgtggc ctttttcttt
     2221 acctggggtt ctccgccaaa atcccagcaa attgcaccac caaagctaaa agcttccagg
     2281 gttgtctaaa aagcacaaga cattagagga agtgcggttt ttataatcaa gccccccaag
     2341 aatttaccat aacatccgtt gcccgcaccg cctcagacgc actcagcgct tttctgacgg
     2401 aaccatgacc aaaaaccaca acaaaccacc ccggaacccg ccagaaacgc tcctgttaaa
     2461 ttttaacggc atgcatgact atgcaccaga atgacggcat gctcttttca c
//
