LOCUS       NZ_AP025857             4760 bp    DNA     circular CON 21-APR-2024
DEFINITION  Enterobacter hormaechei strain FUJ80144 plasmid pFJMB80144_2,
            complete sequence.
ACCESSION   NZ_AP025857
VERSION     NZ_AP025857.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00490873
            Assembly: GCF_023735515.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Rapid phylogenetic analysis of Enterobacter hormaechei using open
            reading frame content patterns acquired by Oxford Nanopore
            sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2022) Contact:Masahiro Suzuki Fujita Health
            University, School of Medicine, Microbiology; Dengakugakubo 1-98,
            Kutsukake-cho, Toyoake, Aichi 470-1192, Japan URL
            :http://www.fujita-hu.ac.jp/~microb/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9b
            Genome Coverage                   :: 82x
            Sequencing Technology             :: MiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023735515.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 05:16:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,296
            CDSs (total)                      :: 5,181
            Genes (coding)                    :: 5,086
            CDSs (with protein)               :: 5,086
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 27 of 95
            Pseudo Genes (incomplete)         :: 72 of 95
            Pseudo Genes (internal stop)      :: 12 of 95
            Pseudo Genes (multiple problems)  :: 14 of 95
            Pseudo Genes (short protein)      :: 1 of 95
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP025857.1.
            Annotated by DFAST v.1.2.17 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="FUJ80144"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pFJMB80144_2"
                     /geo_loc_name="Japan:Aichi"
                     /lat_lon="35.07 N 137.00 E"
                     /collection_date="2019-06-24"
     gene            233..478
                     /locus_tag="NGJ60_RS26395"
     CDS             233..478
                     /locus_tag="NGJ60_RS26395"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023343077.1"
                     /translation="MQPQPAVHDGLPGINARTVGAVVIRLETLASQQLRVRDQEVQLQP
                     PLILVLNPQHAVLVFVESGHQNPFKARHQLFALPGR"
                     /name="hypothetical protein"
     gene            complement(660..983)
                     /locus_tag="NGJ60_RS26400"
                     /old_locus_tag="FJMB80144_51920"
     CDS             complement(660..983)
                     /locus_tag="NGJ60_RS26400"
                     /old_locus_tag="FJMB80144_51920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001445156.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1627..1737
                     /locus_tag="NGJ60_RS26405"
     CDS             1627..1737
                     /locus_tag="NGJ60_RS26405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048246078.1"
                     /translation="MHDPPVQSDYHAHSGNYQLESNPERHDKIAIGGSHW"
                     /name="hypothetical protein"
     gene            complement(1992..2453)
                     /locus_tag="NGJ60_RS26410"
     CDS             complement(1992..2453)
                     /locus_tag="NGJ60_RS26410"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_065707752.1"
                     /translation="MVNTYYGYLLGVTLGEWAMGSDVQWQIDNGYLAQVMGDFSTESPS
                     FETPMFGRYFDTFQAAIHVAKAFPGSTLQPSEYEDKTGMLIRHYYVDCHPYYDRLPPEK
                     SDNFQYDEYPSAIHVAACIDQASKELDELLFGGGYGENGEGHASPIILY"
                     /name="hypothetical protein"
     gene            complement(2853..3767)
                     /gene="lpxO"
                     /locus_tag="NGJ60_RS26415"
     CDS             complement(2853..3767)
                     /gene="lpxO"
                     /locus_tag="NGJ60_RS26415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728425.1"
                     /GO_process="GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A hydroxylase LpxO"
                     /protein_id="WP_023343074.1"
                     /translation="MLSLIIIFVFLISVLYAHSRGKIKQKFSRQLFDHSTFMAPINMFM
                     TGFSKLPSKQAWFPPETFPELQPLIDNWQVIRDEAVNLQGHIKSSEKNNDAGFNTFFKR
                     GWKRFYLKWYGDAHPSAERLCPVTTQLVSSIPCIKAAMFAELPSGSHLGKHRDPYAGSV
                     RYHLGLSTPNDERCFIEVDGVRRSWHDGEVIIFDETFVHWAQNDTEQTRIILFCDIERP
                     MKWRWSQKINRWIGGVLMSAAASPNDNNDRTGAINHLFKYVNAIRDAGQKLKKRNRKLY
                     YLIKNGVISLIFVWLAVSVFRWL"
                     /name="lpxO"
     gene            complement(4038..4253)
                     /locus_tag="NGJ60_RS26420"
                     /old_locus_tag="FJMB80144_51940"
     CDS             complement(4038..4253)
                     /locus_tag="NGJ60_RS26420"
                     /old_locus_tag="FJMB80144_51940"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_023343075.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSDQLRRLSSG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4257..4742)
                     /locus_tag="NGJ60_RS26425"
                     /old_locus_tag="FJMB80144_51950"
     CDS             complement(4257..4742)
                     /locus_tag="NGJ60_RS26425"
                     /old_locus_tag="FJMB80144_51950"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_023343076.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSVLLIASNAAILWWQSQQIL
                     DNYVSIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     Typing          complement(1085..1271)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.316"
                     /coverage="87.0"
                     /evalue="1.25e-50"
     Typing          1251..1737
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.959"
                     /coverage="93.0"
                     /evalue="3.59e-159"
     Typing          complement(3..670)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.309"
                     /coverage="99.0"
                     /evalue="1.93e-141"
     Typing          complement(3941..4758)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="87.179"
                     /coverage="99.0"
                     /evalue="3.28e-146"
ORIGIN
        1 ggatgtttcg ccccccgtcc gggtcggcaa tgctgatgct gctttttgtg gtgcgtacca
       61 cctcaaaacc tgcgctttcc agcgcctcag tgacgtcctg gcgggttttc agctccccgg
      121 acgaggccaa ggccagtaaa ccccgtgtaa ttgcctgagc ggcttcctgc ttcgtttccg
      181 gcagcgcgga cggcgtgacc agcgcccgcc ggttttcagg cgcgttcggg tcatgcagcc
      241 ccagcctgcc gttcacgatg gtctgccagg catcaatgcg cggacggtcg gcgcggtcgt
      301 aatacggctg gagacgcttg ccagtcagca gctccgtgtt cgggatcagg aagttcagct
      361 ccagccgccc cttatcctgg tgctcaaccc acagcacgct gtactggtct ttgtcgagtc
      421 cgggcatcag aacccgttca aagctcgcca tcagcttttc gcgctgcccg ggcggtaaat
      481 ccttttcggc aaaggacagg acgccggatg tgtacttttt tgcgtagggt gaggcgtcga
      541 taagctccct gacttcttcc ggcttcccct gcagaacggt ggcgccctcg cgctgtcggt
      601 ctttccccag cagataatcc accggaccgg cgccaccgcc gcgccctcgc gggtgaaact
      661 taacgatcat cgtctgcacc cttttccagc acggcatgcc gcagccgctc gagtccggca
      721 tctatggcca tcagcgcggc gacgacctgc acgcggtcgt gcccgctgcc accaccggcg
      781 ttaacctgcc gggcaatctg gttaaggttg ttccccatgc cggcaagctg acgaagcagc
      841 gccggcgaga tcgagggaag cttgccggcg cgggcgggtt tctcgtccag gcacgtctgg
      901 cgcatccagg ctgcaagctg tttaccgtcg cagcgctcca gcagacgccg gtgctcatcc
      961 tcggtcaccc acatcgtgag cattttgctg cgtttgtctg ccaatccggc ctcctgataa
     1021 cgccgggacg ggcgggaaat cttttcccgg tcggcgcggt gttggacaag ccgcaggcgc
     1081 gtcagtggtt ttagcgggtt tcggggcgca gccctgaacc agtcacgtag cgctagcgga
     1141 gtgtatactg gcttaatatg gtaaatcgaa gtgggaattc agggaagtgc ttcatgtggc
     1201 aggtaaaaaa tgcgtctgtg gggcgtaagt gcgaaatgct ggggacgatg aaatgacgcc
     1261 tcctcgctca ctcggtcgct acgctcctgc cgtgagactg cggcgggcgt taccggctca
     1321 cgaatggcgg gataaagaga tggcgctcaa atcaggaagg gctgggaaaa ggatgcggcg
     1381 ttgccgtttt tccataggct ccgcccccct gacaagcatc acgaaatatg acgctcaaat
     1441 cagtggcggc gaaacccgac aggactataa agatcccagg cgtttccccc tggtagctcc
     1501 ctcgtgcgct ctcctgttcc tgcctttcgg tttaccggtg tcattccgct gttatgtggc
     1561 cgcgtttgtc tcattccacg cctgacactc agttccgggt aggcagttcg ctccaagctg
     1621 gactgtatgc acgacccccc cgttcagtcc gactaccacg cccattccgg taactatcaa
     1681 cttgagtcca acccggaaag acacgacaaa atcgccattg gcggtagcca ctggtaagca
     1741 ggatgtgcga gagatgtatc gagagttctt gaagtggtgg ccggactacg gctacactgg
     1801 aaggacagat ttggtgacac gtctcgcaca agactgttac cacggttcag aagttcccca
     1861 acttactgaa ccttcgaaaa accacctccc caggtggttt tttcgttttc agagcaagag
     1921 attacgcgca gagcaaaacg atctcaagaa gatcatttta caaattggat aatatccctc
     1981 cgcccctaac actaatataa aattatcggg cttgcatgcc cctcaccatt ttccccatat
     2041 ccaccaccaa acaatagttc gtcgagttct ttgctagctt gatcaataca cgcagccaca
     2101 tgaatagcag aagggtactc atcatactga aaattatcgc ttttttcagg agggagtcga
     2161 tcataatacg gatgacaatc cacataataa tgacgtatta gcatcccagt tttatcttcg
     2221 tactctgatg gctgcaacgt actccctggg aaagcttttg caacatgtat agcagcttga
     2281 aaggtatcaa aatatcgccc aaacattggc gtttcaaagc taggcgactc tgtcgaaaaa
     2341 tcccccataa cttgcgccaa ataaccatta tctatctgcc attgaacatc tgatcccata
     2401 gcccactctc ccagagttac tcctaataaa taaccataat atgtatttac cataacgctt
     2461 cttggccgca ccccctgaga gcccctcagc gcgtttctgc ggcctgaggg ccgtttccgg
     2521 cacctttccc cggcccaaac cctgaaaaac gcccctgata atttctgcgc ggctgcatgg
     2581 ctatgcacaa gagtgaatgg cggccggttt cgtgaaggaa ataccgtgaa agctccgaaa
     2641 gaaacgctta attgcacagc agcacatgaa cgtgatcaga tgaaatctgt ctactgccgc
     2701 acttcttcac gaaaggcccg ctgagcgtca gattcgggcc tttcttacta aaaagatttc
     2761 aatagaattt tttagcaggc gatttttatc tataaccata cattgagcat agaagaccca
     2821 aatttatgta tgactcaata gaaaaaaaca aatcacagcc atctaaacac tgagacagca
     2881 agccaaacaa aaatcaaaga aataactcca tttttaatca aataatatag ttttcgattc
     2941 ctttttttta gcttctgacc tgcatcacga atagcattaa catatttaaa taaatgattt
     3001 atagcaccag tcctgtcatt attatcgttg ggtgatgctg ccgctgacat tagcacccca
     3061 cctatccacc tatttatttt ttgtgaccac cgccacttca tcggcctttc tatatcacag
     3121 aacaaaataa tacgtgtctg ctcggtatca ttctgagccc agtgaacaaa agtttcgtca
     3181 aaaattatca cttcaccatc atgccaactg cggcgtacac catcaacttc aataaaacac
     3241 cgttcatcat ttggtgttga taatcctaaa tgataacgaa ctgaaccggc ataagggtca
     3301 cgatgctttc ctaaatggct tcctgaaggc aattcagcaa acatcgctgc tttaatacag
     3361 ggaatagagc taaccagttg ggttgttact gggcaaagcc gctccgctga tggatgagca
     3421 tctccatacc acttgaggta aaagcgcttc caaccgcgct taaaaaatgt attaaatcct
     3481 gcatcattat ttttttcaga tgatttaatg tgcccttgca aattaacagc ttcgtctctt
     3541 ataacctgcc agttatcaat taatggttga agttcaggaa atgtttctgg gggaaaccat
     3601 gcctgctttg acggcagctt cgaaaagcct gtcataaaca tatttatcgg agccataaat
     3661 gttgaatgat caaataactg cctggaaaac ttttgtttta ttttaccgcg agaatgcgca
     3721 tacaatacac tgataagaaa aacaaaaata ataataagtg acagcatgtg tatattcctt
     3781 gttcacgcca ataataaaaa tctcttaaag ataacataat gaatgcactt aaattataat
     3841 ctaaatgcta tttatcaata gtataatcga agcttcacca gaacatatct actacacagg
     3901 cagagaggat ttcagcagga attatgctga aatgaatcaa agggacggac cgtgatatac
     3961 ccgctgtcgc tgtgatcgtt cgagctccag cgctttctga tgccgcgcct cccgcacctg
     4021 ctgcagctcg acggcattca gccgctcgac aaccggcgca gctgatcgct caaactctgc
     4081 acctgcctgc tcaagcccgt gacgcgctcg ctcagcgccg cgttctcccg ttgcataaga
     4141 ccagacatcg tccgccattc cgcgaaggcg ctctcccact cgtccagcct tttcgagtag
     4201 tcctgctgta gctgctctaa tgcgctcagc aactgttttt ccagctctgt catgtgctat
     4261 ttccccgcca gtatcatcca gctcgaatcc tctccgaacc gtcccgcttc cgggttcacc
     4321 ctcacgcagc ggcgtctctg ctcgccgcag gtcgagagct gcacgccgct gttccgctcg
     4381 gacagcatcg cctgcgtgct cttctgctcc cggatgctca cgtaattgtc gagtatctgc
     4441 tgactctgcc accacagaat ggccgcgttc gaggcgatca gcagcacgga gaccaggacg
     4501 atggtcagcc acgtctggct gaccatgcgc agcatccctt tcgtacgctg gctcatggct
     4561 gaggacagct tccggtcgtg gtcgaggatg gcggcgctga ttctcttctc gctttcgctc
     4621 agttccgctc tgacagactt ctcgtgctcg ctgaatgcgg ctttcagcat ctcgccggta
     4681 ctctgctgct gcgctttcga tttctgctct aagtccttcg ccagcgttaa aagactgttc
     4741 atagatggct cccttcagtc
//
