LOCUS       NZ_AP025854             2511 bp    DNA     circular CON 21-APR-2024
DEFINITION  Enterobacter hormaechei strain FUJ80068 plasmid pFJMB80068_3,
            complete sequence.
ACCESSION   NZ_AP025854
VERSION     NZ_AP025854.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00490872
            Assembly: GCF_023735495.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Rapid phylogenetic analysis of Enterobacter hormaechei using open
            reading frame content patterns acquired by Oxford Nanopore
            sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2022) Contact:Masahiro Suzuki Fujita Health
            University, School of Medicine, Microbiology; Dengakugakubo 1-98,
            Kutsukake-cho, Toyoake, Aichi 470-1192, Japan URL
            :http://www.fujita-hu.ac.jp/~microb/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9b
            Genome Coverage                   :: 567x
            Sequencing Technology             :: MiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023735495.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 05:09:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,146
            CDSs (total)                      :: 5,032
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 24 of 94
            Pseudo Genes (incomplete)         :: 71 of 94
            Pseudo Genes (internal stop)      :: 15 of 94
            Pseudo Genes (multiple problems)  :: 14 of 94
            Pseudo Genes (short protein)      :: 1 of 94
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP025854.1.
            Annotated by DFAST v.1.2.17 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="FUJ80068"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pFJMB80068_3"
                     /geo_loc_name="Japan:Aichi"
                     /lat_lon="35.07 N 137.00 E"
                     /collection_date="2014-05-15"
     gene            complement(270..413)
                     /locus_tag="NGJ26_RS25670"
                     /old_locus_tag="FJMB80068_50500"
     CDS             complement(270..413)
                     /locus_tag="NGJ26_RS25670"
                     /old_locus_tag="FJMB80068_50500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032610644.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHVKTPLAAATGKGYTRLLVQ
                     SS"
                     /name="hypothetical protein"
     gene            complement(1574..1759)
                     /locus_tag="NGJ26_RS25675"
                     /old_locus_tag="FJMB80068_50520"
     CDS             complement(1574..1759)
                     /locus_tag="NGJ26_RS25675"
                     /old_locus_tag="FJMB80068_50520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032105038.1"
                     /translation="MTRQESAALNMAKFIRSQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYTMLNAQTGEEDA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2126..2353)
                     /locus_tag="NGJ26_RS25680"
                     /old_locus_tag="FJMB80068_50530"
     CDS             complement(2126..2353)
                     /locus_tag="NGJ26_RS25680"
                     /old_locus_tag="FJMB80068_50530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     Typing          1448..1527
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.67e-34"
     Typing          complement(249..809)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.339"
                     /coverage="91.0"
                     /evalue="0.0"
ORIGIN
        1 ttatatctga tgtaatccga ccgatacata taccaagatg atttaagtat tcctctttgt
       61 gaggttggat gtctaattta taaaaggatc ttcttgagat cctttttttt cgcgcgtaat
      121 ctcttgccct gtaaacgaaa aaaccacccg gcaaggtggt ttttcgaagg ttcagcaaac
      181 cggcaagttt ctgaaccgtg gtaacagggt gtacaagacc gctgccacca aatttgtcct
      241 tctagtgtag ccgcagtcag gcgtcaactt caagaactct gcaccagtaa tcttgtatac
      301 cccttaccag tggccgctgc cagtggcgtt tttacgtgtc tttccgggtt ggactcaaga
      361 cgatagttac cggataaggc gcagcagtcg gactgaacgg ggggttcgtg catacagtcc
      421 atcctggagc gaactgcctt cccggaaccg agtgtcaggc gtggaatgag aaaccgcggc
      481 cataacagcg gagtgacacc ggtaaaccga aaggcaggaa tgcgggggag cacgagggag
      541 ccatcagggg gaaacgcctg gtatctttaa gccgcatcgg gtttcgccac cactgatttg
      601 agcgtcagat ttcgtgatgc tcgtcagggg ggcggagcct atggaaaaac ggcttcggcg
      661 cggccttatg ccttcttcgc taagtatctt cctggcatcc cctagctggc atcccctagg
      721 aaatttctgc tccacccgta agcccgtacc gctcgccgca gccgaacgac cgagcgcagc
      781 gagtgagtga gcgaggaagc ggaatatatc ctgtattaca ttttctcctg acgcgttttc
      841 tttcattttc tgcgcctgtc ttatgtagca gttatgctat gcgttactgc ggtgttacat
      901 tttagccagt atacactccg ctagcgctga gtgactggtc cggcgctgcg cccggaaccc
      961 gcctgtaccg gttcagcagc cgttccgggc tgactgcaat ttttttattt tcctgcccgc
     1021 taccctgtaa acctttcttc tgcgttgccg ttaacaggag gtaagccatg tttggttttt
     1081 cttccggaga cctgatgact cttgatgatg agcgacacct gtttgtgtct gttgtttcgg
     1141 gctggctccg gcgtgccggg gggatgccgg agccatgatg ctggatgcat accggcagat
     1201 tttgtctgaa actgaaccgg ccgtaaggac ggtcatgctc gaatttcttg aatccgttcg
     1261 cattcattac atttcgtcct gagcattttc tgtatttctg tccatactta aacgaggcgg
     1321 ccgtcacgga cgatggtcgt cgttgcatga tgctgttatg gatgagtgaa agtaatctaa
     1381 tctaacttga cttaccttcc gggcagctcc ccggtttttt tttgcctgtt cccggggaac
     1441 cactcaggac aggatgtgta tttgtaatac cgcgcacgcg ccggcatcac aaatacccag
     1501 gtcagggctg cgccccgaca ccccgccatc gcctcacggc cgcgcactga attgcgcacc
     1561 cgttcaccga ttttcacgca tcttcctcgc ctgtctgtgc gttcagcatg gtgtaaagtg
     1621 cttcggcctg atcgtgcagg tgctcacagc agccggcagc ctcatccaga tccatctgtt
     1681 cgagccgctc gaggaggagg agtgtctgtg agcggataaa tttggccatg ttgagggcgg
     1741 cggattcctg tctggtcact ggtgattttc cccgtctggt gatgattttt gcgtgaaaag
     1801 agcatgccgt cattctggtg catagtcatg catgccgtta aaatttaaca ggagcgtttc
     1861 tggcgggttc cggggtggtt tgttgtggtt tttggtcatg gttccgtcag aaaagcgctg
     1921 agtgcgtctg aggcggtgcg ggcaacggat gttatggtaa attcttgggg ggcttgatta
     1981 taaaaaccgc acttcctcta atgtcttgtg ctttttagac aaccctggaa gcttttagct
     2041 ttggtggtgc aatttgctgg gattttggcg gagaacccca ggtaaagaaa aaggccacat
     2101 tagcggcctt tttcggagaa gatgctcagt gcggaatttc ggagacttct ttaacgtcgg
     2161 ttttattgat ctgctgtttg acaccattcg cgtctgtata gccaagcagg cctgagtctg
     2221 attctttagg tttgccgtcg ctgacaatag tgcgcccgtc attggtgtgt atggcatagc
     2281 ttgtgcgcgt gcagccggtg agggctgaaa gcgcaactgc cgctgcgaac acggaaacaa
     2341 aaatcttttt caaagccagc tccttttatc catatcagtt ttggtcacta accatctaac
     2401 tataggtcat tttttatgtg aaaagaggct tgatggtggg ctgcatatcg agttaggtgc
     2461 gaggtgttat ggttaaagtg tggtgtctgg cggggagggg gcagtgcggg c
//
