LOCUS       NZ_AP025853             4760 bp    DNA     circular CON 21-APR-2024
DEFINITION  Enterobacter hormaechei strain FUJ80068 plasmid pFJMB80068_2,
            complete sequence.
ACCESSION   NZ_AP025853
VERSION     NZ_AP025853.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00490872
            Assembly: GCF_023735495.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Rapid phylogenetic analysis of Enterobacter hormaechei using open
            reading frame content patterns acquired by Oxford Nanopore
            sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2022) Contact:Masahiro Suzuki Fujita Health
            University, School of Medicine, Microbiology; Dengakugakubo 1-98,
            Kutsukake-cho, Toyoake, Aichi 470-1192, Japan URL
            :http://www.fujita-hu.ac.jp/~microb/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9b
            Genome Coverage                   :: 567x
            Sequencing Technology             :: MiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023735495.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 05:09:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,146
            CDSs (total)                      :: 5,032
            Genes (coding)                    :: 4,938
            CDSs (with protein)               :: 4,938
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 24 of 94
            Pseudo Genes (incomplete)         :: 71 of 94
            Pseudo Genes (internal stop)      :: 15 of 94
            Pseudo Genes (multiple problems)  :: 14 of 94
            Pseudo Genes (short protein)      :: 1 of 94
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP025853.1.
            Annotated by DFAST v.1.2.17 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="FUJ80068"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pFJMB80068_2"
                     /geo_loc_name="Japan:Aichi"
                     /lat_lon="35.07 N 137.00 E"
                     /collection_date="2014-05-15"
     gene            complement(join(4665..4760,1..390))
                     /locus_tag="NGJ26_RS25635"
     CDS             complement(join(4665..4760,1..390))
                     /locus_tag="NGJ26_RS25635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_023343076.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSVLLIASNAAILWWQSQQIL
                     DNYVSIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(<425..1129)
                     /locus_tag="NGJ26_RS25755"
                     /pseudo=""
     CDS             complement(<425..1129)
                     /locus_tag="NGJ26_RS25755"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1068..1391)
                     /locus_tag="NGJ26_RS25645"
                     /old_locus_tag="FJMB80068_50470"
     CDS             complement(1068..1391)
                     /locus_tag="NGJ26_RS25645"
                     /old_locus_tag="FJMB80068_50470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001445156.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2035..2145
                     /locus_tag="NGJ26_RS25650"
     CDS             2035..2145
                     /locus_tag="NGJ26_RS25650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048246078.1"
                     /translation="MHDPPVQSDYHAHSGNYQLESNPERHDKIAIGGSHW"
                     /name="hypothetical protein"
     gene            complement(2400..2861)
                     /locus_tag="NGJ26_RS25655"
     CDS             complement(2400..2861)
                     /locus_tag="NGJ26_RS25655"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_065707752.1"
                     /translation="MVNTYYGYLLGVTLGEWAMGSDVQWQIDNGYLAQVMGDFSTESPS
                     FETPMFGRYFDTFQAAIHVAKAFPGSTLQPSEYEDKTGMLIRHYYVDCHPYYDRLPPEK
                     SDNFQYDEYPSAIHVAACIDQASKELDELLFGGGYGENGEGHASPIILY"
                     /name="hypothetical protein"
     gene            complement(3261..4175)
                     /gene="lpxO"
                     /locus_tag="NGJ26_RS25660"
     CDS             complement(3261..4175)
                     /gene="lpxO"
                     /locus_tag="NGJ26_RS25660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728425.1"
                     /GO_process="GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A hydroxylase LpxO"
                     /protein_id="WP_023343074.1"
                     /translation="MLSLIIIFVFLISVLYAHSRGKIKQKFSRQLFDHSTFMAPINMFM
                     TGFSKLPSKQAWFPPETFPELQPLIDNWQVIRDEAVNLQGHIKSSEKNNDAGFNTFFKR
                     GWKRFYLKWYGDAHPSAERLCPVTTQLVSSIPCIKAAMFAELPSGSHLGKHRDPYAGSV
                     RYHLGLSTPNDERCFIEVDGVRRSWHDGEVIIFDETFVHWAQNDTEQTRIILFCDIERP
                     MKWRWSQKINRWIGGVLMSAAASPNDNNDRTGAINHLFKYVNAIRDAGQKLKKRNRKLY
                     YLIKNGVISLIFVWLAVSVFRWL"
                     /name="lpxO"
     gene            complement(4446..4661)
                     /locus_tag="NGJ26_RS25665"
                     /old_locus_tag="FJMB80068_50490"
     CDS             complement(4446..4661)
                     /locus_tag="NGJ26_RS25665"
                     /old_locus_tag="FJMB80068_50490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_023343075.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSDQLRRLSSG"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          complement(1493..1679)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.316"
                     /coverage="87.0"
                     /evalue="1.25e-50"
     Typing          1659..2145
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.959"
                     /coverage="93.0"
                     /evalue="3.59e-159"
     Typing          complement(60..1078)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.588"
                     /coverage="89.0"
                     /evalue="0.0"
     Typing          complement(4349..4758)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="85.401"
                     /coverage="99.0"
                     /evalue="2.14e-61"
ORIGIN
        1 cgagagctgc acgccgctgt tccgctcgga cagcatcgcc tgcgtgctct tctgctcccg
       61 gatgctcacg taattgtcga gtatctgctg actctgccac cacagaatgg ccgcgttcga
      121 ggcgatcagc agcacggaga ccaggacgat ggtcagccac gtctggctga ccatgcgcag
      181 catccctttc gtacgctggc tcatggctga ggacagcttc cggtcgtggt cgaggatggc
      241 ggcgctgatt ctcttctcgc tttcgctcag ttccgctctg acagacttct cgtgctcgct
      301 gaatgcggct ttcagcatct cgccggtact ctgctgctgc gctttcgatt tctgctctaa
      361 gtccttcgcc agcgttaaaa gactgttcat agatggctcc cttcagtcgg atgtttcgcc
      421 ccccgtccgg gtcggcaatg ctgatgctgc tttttgtggt gcgtaccacc tcaaaacctg
      481 cgctttccag cgcctcagtg acgtcctggc gggttttcag ctccccggac gaggccaagg
      541 ccagtaaacc ccgtgtaatt gcctgagcgg cttcctgctt cgtttccggc agcgcggacg
      601 gcgtgaccag cgcccgccgg ttttcaggcg cgttcgggtc atgcagcccc agcctgccgt
      661 tcacgatggt ctgccaggca tcaatgcgcg gacggtcggc gcggtcgtaa tacggctgga
      721 gacgcttgcc agtcagcagc tccgtgttcg ggatcaggaa gttcagctcc agccgcccct
      781 tatcctggtg ctcaacccac agcacgctgt actggtcttt gtcgagtccg ggcatcagaa
      841 cccgttcaaa gctcgccatc agcttttcgc gctgcccggg cggtaaatcc ttttcggcaa
      901 aggacaggac gccggatgtg tacttttttg cgtagggtga ggcgtcgata agctccctga
      961 cttcttccgg cttcccctgc agaacggtgg cgccctcgcg ctgtcggtct ttccccagca
     1021 gataatccac cggaccggcg ccaccgccgc gccctcgcgg gtgaaactta acgatcatcg
     1081 tctgcaccct tttccagcac ggcatgccgc agccgctcga gtccggcatc tatggccatc
     1141 agcgcggcga cgacctgcac gcggtcgtgc ccgctgccac caccggcgtt aacctgccgg
     1201 gcaatctggt taaggttgtt ccccatgccg gcaagctgac gaagcagcgc cggcgagatc
     1261 gagggaagct tgccggcgcg ggcgggtttc tcgtccaggc acgtctggcg catccaggct
     1321 gcaagctgtt taccgtcgca gcgctccagc agacgccggt gctcatcctc ggtcacccac
     1381 atcgtgagca ttttgctgcg tttgtctgcc aatccggcct cctgataacg ccgggacggg
     1441 cgggaaatct tttcccggtc ggcgcggtgt tggacaagcc gcaggcgcgt cagtggtttt
     1501 agcgggtttc ggggcgcagc cctgaaccag tcacgtagcg ctagcggagt gtatactggc
     1561 ttaatatggt aaatcgaagt gggaattcag ggaagtgctt catgtggcag gtaaaaaatg
     1621 cgtctgtggg gcgtaagtgc gaaatgctgg ggacgatgaa atgacgcctc ctcgctcact
     1681 cggtcgctac gctcctgccg tgagactgcg gcgggcgtta ccggctcacg aatggcggga
     1741 taaagagatg gcgctcaaat caggaagggc tgggaaaagg atgcggcgtt gccgtttttc
     1801 cataggctcc gcccccctga caagcatcac gaaatatgac gctcaaatca gtggcggcga
     1861 aacccgacag gactataaag atcccaggcg tttccccctg gtagctccct cgtgcgctct
     1921 cctgttcctg cctttcggtt taccggtgtc attccgctgt tatgtggccg cgtttgtctc
     1981 attccacgcc tgacactcag ttccgggtag gcagttcgct ccaagctgga ctgtatgcac
     2041 gacccccccg ttcagtccga ctaccacgcc cattccggta actatcaact tgagtccaac
     2101 ccggaaagac acgacaaaat cgccattggc ggtagccact ggtaagcagg atgtgcgaga
     2161 gatgtatcga gagttcttga agtggtggcc ggactacggc tacactggaa ggacagattt
     2221 ggtgacacgt ctcgcacaag actgttacca cggttcagaa gttccccaac ttactgaacc
     2281 ttcgaaaaac cacctcccca ggtggttttt tcgttttcag agcaagagat tacgcgcaga
     2341 gcaaaacgat ctcaagaaga tcattttaca aattggataa tatccctccg cccctaacac
     2401 taatataaaa ttatcgggct tgcatgcccc tcaccatttt ccccatatcc accaccaaac
     2461 aatagttcgt cgagttcttt gctagcttga tcaatacacg cagccacatg aatagcagaa
     2521 gggtactcat catactgaaa attatcgctt ttttcaggag ggagtcgatc ataatacgga
     2581 tgacaatcca cataataatg acgtattagc atcccagttt tatcttcgta ctctgatggc
     2641 tgcaacgtac tccctgggaa agcttttgca acatgtatag cagcttgaaa ggtatcaaaa
     2701 tatcgcccaa acattggcgt ttcaaagcta ggcgactctg tcgaaaaatc ccccataact
     2761 tgcgccaaat aaccattatc tatctgccat tgaacatctg atcccatagc ccactctccc
     2821 agagttactc ctaataaata accataatat gtatttacca taacgcttct tggccgcacc
     2881 ccctgagagc ccctcagcgc gtttctgcgg cctgagggcc gtttccggca cctttccccg
     2941 gcccaaaccc tgaaaaacgc ccctgataat ttctgcgcgg ctgcatggct atgcacaaga
     3001 gtgaatggcg gccggtttcg tgaaggaaat accgtgaaag ctccgaaaga aacgcttaat
     3061 tgcacagcag cacatgaacg tgatcagatg aaatctgtct actgccgcac ttcttcacga
     3121 aaggcccgct gagcgtcaga ttcgggcctt tcttactaaa aagatttcaa tagaattttt
     3181 tagcaggcga tttttatcta taaccataca ttgagcatag aagacccaaa tttatgtatg
     3241 actcaataga aaaaaacaaa tcacagccat ctaaacactg agacagcaag ccaaacaaaa
     3301 atcaaagaaa taactccatt tttaatcaaa taatatagtt ttcgattcct tttttttagc
     3361 ttctgacctg catcacgaat agcattaaca tatttaaata aatgatttat agcaccagtc
     3421 ctgtcattat tatcgttggg tgatgctgcc gctgacatta gcaccccacc tatccaccta
     3481 tttatttttt gtgaccaccg ccacttcatc ggcctttcta tatcacagaa caaaataata
     3541 cgtgtctgct cggtatcatt ctgagcccag tgaacaaaag tttcgtcaaa aattatcact
     3601 tcaccatcat gccaactgcg gcgtacacca tcaacttcaa taaaacaccg ttcatcattt
     3661 ggtgttgata atcctaaatg ataacgaact gaaccggcat aagggtcacg atgctttcct
     3721 aaatggcttc ctgaaggcaa ttcagcaaac atcgctgctt taatacaggg aatagagcta
     3781 accagttggg ttgttactgg gcaaagccgc tccgctgatg gatgagcatc tccataccac
     3841 ttgaggtaaa agcgcttcca accgcgctta aaaaatgtat taaatcctgc atcattattt
     3901 ttttcagatg atttaatgtg cccttgcaaa ttaacagctt cgtctcttat aacctgccag
     3961 ttatcaatta atggttgaag ttcaggaaat gtttctgggg gaaaccatgc ctgctttgac
     4021 ggcagcttcg aaaagcctgt cataaacata tttatcggag ccataaatgt tgaatgatca
     4081 aataactgcc tggaaaactt ttgttttatt ttaccgcgag aatgcgcata caatacactg
     4141 ataagaaaaa caaaaataat aataagtgac agcatgtgta tattccttgt tcacgccaat
     4201 aataaaaatc tcttaaagat aacataatga atgcacttaa attataatct aaatgctatt
     4261 tatcaatagt ataatcgaag cttcaccaga acatatctac tacacaggca gagaggattt
     4321 cagcaggaat tatgctgaaa tgaatcaaag ggacggaccg tgatataccc gctgtcgctg
     4381 tgatcgttcg agctccagcg ctttctgatg ccgcgcctcc cgcacctgct gcagctcgac
     4441 ggcattcagc cgctcgacaa ccggcgcagc tgatcgctca aactctgcac ctgcctgctc
     4501 aagcccgtga cgcgctcgct cagcgccgcg ttctcccgtt gcataagacc agacatcgtc
     4561 cgccattccg cgaaggcgct ctcccactcg tccagccttt tcgagtagtc ctgctgtagc
     4621 tgctctaatg cgctcagcaa ctgtttttcc agctctgtca tgtgctattt ccccgccagt
     4681 atcatccagc tcgaatcctc tccgaaccgt cccgcttccg ggttcaccct cacgcagcgg
     4741 cgtctctgct cgccgcaggt
//
