LOCUS       NZ_AP025850             2511 bp    DNA     circular CON 22-APR-2024
DEFINITION  Enterobacter hormaechei strain FUJ80063 plasmid pFJMB0063_3,
            complete sequence.
ACCESSION   NZ_AP025850
VERSION     NZ_AP025850.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00490871
            Assembly: GCF_023735475.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Rapid phylogenetic analysis of Enterobacter hormaechei using open
            reading frame content patterns acquired by Oxford Nanopore
            sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2022) Contact:Masahiro Suzuki Fujita Health
            University, School of Medicine, Microbiology; Dengakugakubo 1-98,
            Kutsukake-cho, Toyoake, Aichi 470-1192, Japan URL
            :http://www.fujita-hu.ac.jp/~microb/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9b
            Genome Coverage                   :: 272x
            Sequencing Technology             :: MiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023735475.1-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 05:01:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,207
            CDSs (total)                      :: 5,093
            Genes (coding)                    :: 4,998
            CDSs (with protein)               :: 4,998
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 25 of 95
            Pseudo Genes (incomplete)         :: 71 of 95
            Pseudo Genes (internal stop)      :: 15 of 95
            Pseudo Genes (multiple problems)  :: 14 of 95
            Pseudo Genes (short protein)      :: 1 of 95
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP025850.1.
            Annotated by DFAST v.1.2.17 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="FUJ80063"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pFJMB0063_3"
                     /geo_loc_name="Japan:Aichi"
                     /lat_lon="35.07 N 137.00 E"
                     /collection_date="2015-09-12"
     gene            complement(51..236)
                     /locus_tag="NGK57_RS25975"
                     /old_locus_tag="FJMB80063_51130"
     CDS             complement(51..236)
                     /locus_tag="NGK57_RS25975"
                     /old_locus_tag="FJMB80063_51130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032105038.1"
                     /translation="MTRQESAALNMAKFIRSQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYTMLNAQTGEEDA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(603..830)
                     /locus_tag="NGK57_RS25980"
                     /old_locus_tag="FJMB80063_51140"
     CDS             complement(603..830)
                     /locus_tag="NGK57_RS25980"
                     /old_locus_tag="FJMB80063_51140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            complement(1258..1401)
                     /locus_tag="NGK57_RS25985"
                     /old_locus_tag="FJMB80063_51150"
     CDS             complement(1258..1401)
                     /locus_tag="NGK57_RS25985"
                     /old_locus_tag="FJMB80063_51150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032610644.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHVKTPLAAATGKGYTRLLVQ
                     SS"
                     /name="hypothetical protein"
     Typing          complement(1237..1797)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.339"
                     /coverage="91.0"
                     /evalue="0.0"
ORIGIN
        1 cgccatcgcc tcacggccgc gcactgaatt gcgcacccgt tcaccgattt tcacgcatct
       61 tcctcgcctg tctgtgcgtt cagcatggtg taaagtgctt cggcctgatc gtgcaggtgc
      121 tcacagcagc cggcagcctc atccagatcc atctgttcga gccgctcgag gaggaggagt
      181 gtctgtgagc ggataaattt ggccatgttg agggcggcgg attcctgtct ggtcactggt
      241 gattttcccc gtctggtgat gatttttgcg tgaaaagagc atgccgtcat tctggtgcat
      301 agtcatgcat gccgttaaaa tttaacagga gcgtttctgg cgggttccgg ggtggtttgt
      361 tgtggttttt ggtcatggtt ccgtcagaaa agcgctgagt gcgtctgagg cggtgcgggc
      421 aacggatgtt atggtaaatt cttggggggc ttgattataa aaaccgcact tcctctaatg
      481 tcttgtgctt tttagacaac cctggaagct tttagctttg gtggtgcaat ttgctgggat
      541 tttggcggag aaccccaggt aaagaaaaag gccacattag cggccttttt cggagaagat
      601 gctcagtgcg gaatttcgga gacttcttta acgtcggttt tattgatctg ctgtttgaca
      661 ccattcgcgt ctgtatagcc aagcaggcct gagtctgatt ctttaggttt gccgtcgctg
      721 acaatagtgc gcccgtcatt ggtgtgtatg gcatagcttg tgcgcgtgca gccggtgagg
      781 gctgaaagcg caactgccgc tgcgaacacg gaaacaaaaa tctttttcaa agccagctcc
      841 ttttatccat atcagttttg gtcactaacc atctaactat aggtcatttt ttatgtgaaa
      901 agaggcttga tggtgggctg catatcgagt taggtgcgag gtgttatggt taaagtgtgg
      961 tgtctggcgg ggagggggca gtgcgggctt atatctgatg taatccgacc gatacatata
     1021 ccaagatgat ttaagtattc ctctttgtga ggttggatgt ctaatttata aaaggatctt
     1081 cttgagatcc ttttttttcg cgcgtaatct cttgccctgt aaacgaaaaa accacccggc
     1141 aaggtggttt ttcgaaggtt cagcaaaccg gcaagtttct gaaccgtggt aacagggtgt
     1201 acaagaccgc tgccaccaaa tttgtccttc tagtgtagcc gcagtcaggc gtcaacttca
     1261 agaactctgc accagtaatc ttgtataccc cttaccagtg gccgctgcca gtggcgtttt
     1321 tacgtgtctt tccgggttgg actcaagacg atagttaccg gataaggcgc agcagtcgga
     1381 ctgaacgggg ggttcgtgca tacagtccat cctggagcga actgccttcc cggaaccgag
     1441 tgtcaggcgt ggaatgagaa accgcggcca taacagcgga gtgacaccgg taaaccgaaa
     1501 ggcaggaatg cgggggagca cgagggagcc atcaggggga aacgcctggt atctttaagc
     1561 cgcatcgggt ttcgccacca ctgatttgag cgtcagattt cgtgatgctc gtcagggggg
     1621 cggagcctat ggaaaaacgg cttcggcgcg gccttatgcc ttcttcgcta agtatcttcc
     1681 tggcatcccc tagctggcat cccctaggaa atttctgctc cacccgtaag cccgtaccgc
     1741 tcgccgcagc cgaacgaccg agcgcagcga gtgagtgagc gaggaagcgg aatatatcct
     1801 gtattacatt ttctcctgac gcgttttctt tcattttctg cgcctgtctt atgtagcagt
     1861 tatgctatgc gttactgcgg tgttacattt tagccagtat acactccgct agcgctgagt
     1921 gactggtccg gcgctgcgcc cggaacccgc ctgtaccggt tcagcagccg ttccgggctg
     1981 actgcaattt ttttattttc ctgcccgcta ccctgtaaac ctttcttctg cgttgccgtt
     2041 aacaggaggt aagccatgtt tggtttttct tccggagacc tgatgactct tgatgatgag
     2101 cgacacctgt ttgtgtctgt tgtttcgggc tggctccggc gtgccggggg gatgccggag
     2161 ccatgatgct ggatgcatac cggcagattt tgtctgaaac tgaaccggcc gtaaggacgg
     2221 tcatgctcga atttcttgaa tccgttcgca ttcattacat ttcgtcctga gcattttctg
     2281 tatttctgtc catacttaaa cgaggcggcc gtcacggacg atggtcgtcg ttgcatgatg
     2341 ctgttatgga tgagtgaaag taatctaatc taacttgact taccttccgg gcagctcccc
     2401 ggtttttttt tgcctgttcc cggggaacca ctcaggacag gatgtgtatt tgtaataccg
     2461 cgcacgcgcc ggcatcacaa atacccaggt cagggctgcg ccccgacacc c
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