LOCUS       NZ_AP025849             4760 bp    DNA     circular CON 22-APR-2024
DEFINITION  Enterobacter hormaechei strain FUJ80063 plasmid pFJMB80063_2,
            complete sequence.
ACCESSION   NZ_AP025849
VERSION     NZ_AP025849.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00490871
            Assembly: GCF_023735475.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Rapid phylogenetic analysis of Enterobacter hormaechei using open
            reading frame content patterns acquired by Oxford Nanopore
            sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2022) Contact:Masahiro Suzuki Fujita Health
            University, School of Medicine, Microbiology; Dengakugakubo 1-98,
            Kutsukake-cho, Toyoake, Aichi 470-1192, Japan URL
            :http://www.fujita-hu.ac.jp/~microb/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9b
            Genome Coverage                   :: 272x
            Sequencing Technology             :: MiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023735475.1-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 05:01:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,207
            CDSs (total)                      :: 5,093
            Genes (coding)                    :: 4,998
            CDSs (with protein)               :: 4,998
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 25 of 95
            Pseudo Genes (incomplete)         :: 71 of 95
            Pseudo Genes (internal stop)      :: 15 of 95
            Pseudo Genes (multiple problems)  :: 14 of 95
            Pseudo Genes (short protein)      :: 1 of 95
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP025849.1.
            Annotated by DFAST v.1.2.17 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="FUJ80063"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pFJMB80063_2"
                     /geo_loc_name="Japan:Aichi"
                     /lat_lon="35.07 N 137.00 E"
                     /collection_date="2015-09-12"
     gene            66..176
                     /locus_tag="NGK57_RS25940"
     CDS             66..176
                     /locus_tag="NGK57_RS25940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048246078.1"
                     /translation="MHDPPVQSDYHAHSGNYQLESNPERHDKIAIGGSHW"
                     /name="hypothetical protein"
     gene            complement(431..892)
                     /locus_tag="NGK57_RS25945"
     CDS             complement(431..892)
                     /locus_tag="NGK57_RS25945"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_065707752.1"
                     /translation="MVNTYYGYLLGVTLGEWAMGSDVQWQIDNGYLAQVMGDFSTESPS
                     FETPMFGRYFDTFQAAIHVAKAFPGSTLQPSEYEDKTGMLIRHYYVDCHPYYDRLPPEK
                     SDNFQYDEYPSAIHVAACIDQASKELDELLFGGGYGENGEGHASPIILY"
                     /name="hypothetical protein"
     gene            complement(1292..2206)
                     /gene="lpxO"
                     /locus_tag="NGK57_RS25950"
     CDS             complement(1292..2206)
                     /gene="lpxO"
                     /locus_tag="NGK57_RS25950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728425.1"
                     /GO_process="GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A hydroxylase LpxO"
                     /protein_id="WP_023343074.1"
                     /translation="MLSLIIIFVFLISVLYAHSRGKIKQKFSRQLFDHSTFMAPINMFM
                     TGFSKLPSKQAWFPPETFPELQPLIDNWQVIRDEAVNLQGHIKSSEKNNDAGFNTFFKR
                     GWKRFYLKWYGDAHPSAERLCPVTTQLVSSIPCIKAAMFAELPSGSHLGKHRDPYAGSV
                     RYHLGLSTPNDERCFIEVDGVRRSWHDGEVIIFDETFVHWAQNDTEQTRIILFCDIERP
                     MKWRWSQKINRWIGGVLMSAAASPNDNNDRTGAINHLFKYVNAIRDAGQKLKKRNRKLY
                     YLIKNGVISLIFVWLAVSVFRWL"
                     /name="lpxO"
     gene            complement(2477..2692)
                     /locus_tag="NGK57_RS25955"
                     /old_locus_tag="FJMB80063_51100"
     CDS             complement(2477..2692)
                     /locus_tag="NGK57_RS25955"
                     /old_locus_tag="FJMB80063_51100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_023343075.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSDQLRRLSSG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2696..3181)
                     /locus_tag="NGK57_RS25960"
                     /old_locus_tag="FJMB80063_51110"
     CDS             complement(2696..3181)
                     /locus_tag="NGK57_RS25960"
                     /old_locus_tag="FJMB80063_51110"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_023343076.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSVLLIASNAAILWWQSQQIL
                     DNYVSIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(<3216..3920)
                     /locus_tag="NGK57_RS26060"
                     /pseudo=""
     CDS             complement(<3216..3920)
                     /locus_tag="NGK57_RS26060"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3859..4182)
                     /locus_tag="NGK57_RS25970"
                     /old_locus_tag="FJMB80063_51120"
     CDS             complement(3859..4182)
                     /locus_tag="NGK57_RS25970"
                     /old_locus_tag="FJMB80063_51120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001445156.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(4284..4470)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.316"
                     /coverage="87.0"
                     /evalue="1.25e-50"
     Typing          2..393
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="83.455"
                     /coverage="99.0"
                     /evalue="1.85e-97"
     Typing          4450..4755
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.379"
                     /coverage="92.0"
                     /evalue="5.09e-93"
     Typing          complement(2380..3869)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.182"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gcgtttgtct cattccacgc ctgacactca gttccgggta ggcagttcgc tccaagctgg
       61 actgtatgca cgaccccccc gttcagtccg actaccacgc ccattccggt aactatcaac
      121 ttgagtccaa cccggaaaga cacgacaaaa tcgccattgg cggtagccac tggtaagcag
      181 gatgtgcgag agatgtatcg agagttcttg aagtggtggc cggactacgg ctacactgga
      241 aggacagatt tggtgacacg tctcgcacaa gactgttacc acggttcaga agttccccaa
      301 cttactgaac cttcgaaaaa ccacctcccc aggtggtttt ttcgttttca gagcaagaga
      361 ttacgcgcag agcaaaacga tctcaagaag atcattttac aaattggata atatccctcc
      421 gcccctaaca ctaatataaa attatcgggc ttgcatgccc ctcaccattt tccccatatc
      481 caccaccaaa caatagttcg tcgagttctt tgctagcttg atcaatacac gcagccacat
      541 gaatagcaga agggtactca tcatactgaa aattatcgct tttttcagga gggagtcgat
      601 cataatacgg atgacaatcc acataataat gacgtattag catcccagtt ttatcttcgt
      661 actctgatgg ctgcaacgta ctccctggga aagcttttgc aacatgtata gcagcttgaa
      721 aggtatcaaa atatcgccca aacattggcg tttcaaagct aggcgactct gtcgaaaaat
      781 cccccataac ttgcgccaaa taaccattat ctatctgcca ttgaacatct gatcccatag
      841 cccactctcc cagagttact cctaataaat aaccataata tgtatttacc ataacgcttc
      901 ttggccgcac cccctgagag cccctcagcg cgtttctgcg gcctgagggc cgtttccggc
      961 acctttcccc ggcccaaacc ctgaaaaacg cccctgataa tttctgcgcg gctgcatggc
     1021 tatgcacaag agtgaatggc ggccggtttc gtgaaggaaa taccgtgaaa gctccgaaag
     1081 aaacgcttaa ttgcacagca gcacatgaac gtgatcagat gaaatctgtc tactgccgca
     1141 cttcttcacg aaaggcccgc tgagcgtcag attcgggcct ttcttactaa aaagatttca
     1201 atagaatttt ttagcaggcg atttttatct ataaccatac attgagcata gaagacccaa
     1261 atttatgtat gactcaatag aaaaaaacaa atcacagcca tctaaacact gagacagcaa
     1321 gccaaacaaa aatcaaagaa ataactccat ttttaatcaa ataatatagt tttcgattcc
     1381 ttttttttag cttctgacct gcatcacgaa tagcattaac atatttaaat aaatgattta
     1441 tagcaccagt cctgtcatta ttatcgttgg gtgatgctgc cgctgacatt agcaccccac
     1501 ctatccacct atttattttt tgtgaccacc gccacttcat cggcctttct atatcacaga
     1561 acaaaataat acgtgtctgc tcggtatcat tctgagccca gtgaacaaaa gtttcgtcaa
     1621 aaattatcac ttcaccatca tgccaactgc ggcgtacacc atcaacttca ataaaacacc
     1681 gttcatcatt tggtgttgat aatcctaaat gataacgaac tgaaccggca taagggtcac
     1741 gatgctttcc taaatggctt cctgaaggca attcagcaaa catcgctgct ttaatacagg
     1801 gaatagagct aaccagttgg gttgttactg ggcaaagccg ctccgctgat ggatgagcat
     1861 ctccatacca cttgaggtaa aagcgcttcc aaccgcgctt aaaaaatgta ttaaatcctg
     1921 catcattatt tttttcagat gatttaatgt gcccttgcaa attaacagct tcgtctctta
     1981 taacctgcca gttatcaatt aatggttgaa gttcaggaaa tgtttctggg ggaaaccatg
     2041 cctgctttga cggcagcttc gaaaagcctg tcataaacat atttatcgga gccataaatg
     2101 ttgaatgatc aaataactgc ctggaaaact tttgttttat tttaccgcga gaatgcgcat
     2161 acaatacact gataagaaaa acaaaaataa taataagtga cagcatgtgt atattccttg
     2221 ttcacgccaa taataaaaat ctcttaaaga taacataatg aatgcactta aattataatc
     2281 taaatgctat ttatcaatag tataatcgaa gcttcaccag aacatatcta ctacacaggc
     2341 agagaggatt tcagcaggaa ttatgctgaa atgaatcaaa gggacggacc gtgatatacc
     2401 cgctgtcgct gtgatcgttc gagctccagc gctttctgat gccgcgcctc ccgcacctgc
     2461 tgcagctcga cggcattcag ccgctcgaca accggcgcag ctgatcgctc aaactctgca
     2521 cctgcctgct caagcccgtg acgcgctcgc tcagcgccgc gttctcccgt tgcataagac
     2581 cagacatcgt ccgccattcc gcgaaggcgc tctcccactc gtccagcctt ttcgagtagt
     2641 cctgctgtag ctgctctaat gcgctcagca actgtttttc cagctctgtc atgtgctatt
     2701 tccccgccag tatcatccag ctcgaatcct ctccgaaccg tcccgcttcc gggttcaccc
     2761 tcacgcagcg gcgtctctgc tcgccgcagg tcgagagctg cacgccgctg ttccgctcgg
     2821 acagcatcgc ctgcgtgctc ttctgctccc ggatgctcac gtaattgtcg agtatctgct
     2881 gactctgcca ccacagaatg gccgcgttcg aggcgatcag cagcacggag accaggacga
     2941 tggtcagcca cgtctggctg accatgcgca gcatcccttt cgtacgctgg ctcatggctg
     3001 aggacagctt ccggtcgtgg tcgaggatgg cggcgctgat tctcttctcg ctttcgctca
     3061 gttccgctct gacagacttc tcgtgctcgc tgaatgcggc tttcagcatc tcgccggtac
     3121 tctgctgctg cgctttcgat ttctgctcta agtccttcgc cagcgttaaa agactgttca
     3181 tagatggctc ccttcagtcg gatgtttcgc cccccgtccg ggtcggcaat gctgatgctg
     3241 ctttttgtgg tgcgtaccac ctcaaaacct gcgctttcca gcgcctcagt gacgtcctgg
     3301 cgggttttca gctccccgga cgaggccaag gccagtaaac cccgtgtaat tgcctgagcg
     3361 gcttcctgct tcgtttccgg cagcgcggac ggcgtgacca gcgcccgccg gttttcaggc
     3421 gcgttcgggt catgcagccc cagcctgccg ttcacgatgg tctgccaggc atcaatgcgc
     3481 ggacggtcgg cgcggtcgta atacggctgg agacgcttgc cagtcagcag ctccgtgttc
     3541 gggatcagga agttcagctc cagccgcccc ttatcctggt gctcaaccca cagcacgctg
     3601 tactggtctt tgtcgagtcc gggcatcaga acccgttcaa agctcgccat cagcttttcg
     3661 cgctgcccgg gcggtaaatc cttttcggca aaggacagga cgccggatgt gtactttttt
     3721 gcgtagggtg aggcgtcgat aagctccctg acttcttccg gcttcccctg cagaacggtg
     3781 gcgccctcgc gctgtcggtc tttccccagc agataatcca ccggaccggc gccaccgccg
     3841 cgccctcgcg ggtgaaactt aacgatcatc gtctgcaccc ttttccagca cggcatgccg
     3901 cagccgctcg agtccggcat ctatggccat cagcgcggcg acgacctgca cgcggtcgtg
     3961 cccgctgcca ccaccggcgt taacctgccg ggcaatctgg ttaaggttgt tccccatgcc
     4021 ggcaagctga cgaagcagcg ccggcgagat cgagggaagc ttgccggcgc gggcgggttt
     4081 ctcgtccagg cacgtctggc gcatccaggc tgcaagctgt ttaccgtcgc agcgctccag
     4141 cagacgccgg tgctcatcct cggtcaccca catcgtgagc attttgctgc gtttgtctgc
     4201 caatccggcc tcctgataac gccgggacgg gcgggaaatc ttttcccggt cggcgcggtg
     4261 ttggacaagc cgcaggcgcg tcagtggttt tagcgggttt cggggcgcag ccctgaacca
     4321 gtcacgtagc gctagcggag tgtatactgg cttaatatgg taaatcgaag tgggaattca
     4381 gggaagtgct tcatgtggca ggtaaaaaat gcgtctgtgg ggcgtaagtg cgaaatgctg
     4441 gggacgatga aatgacgcct cctcgctcac tcggtcgcta cgctcctgcc gtgagactgc
     4501 ggcgggcgtt accggctcac gaatggcggg ataaagagat ggcgctcaaa tcaggaaggg
     4561 ctgggaaaag gatgcggcgt tgccgttttt ccataggctc cgcccccctg acaagcatca
     4621 cgaaatatga cgctcaaatc agtggcggcg aaacccgaca ggactataaa gatcccaggc
     4681 gtttccccct ggtagctccc tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt
     4741 cattccgctg ttatgtggcc
//
