LOCUS       NZ_AP025846             2495 bp    DNA     circular CON 22-APR-2024
DEFINITION  Enterobacter hormaechei strain FUJ80379 plasmid pFJMB80379_4,
            complete sequence.
ACCESSION   NZ_AP025846
VERSION     NZ_AP025846.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00490869
            Assembly: GCF_024182025.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Rapid phylogenetic analysis of Enterobacter hormaechei using open
            reading frame content patterns acquired by Oxford Nanopore
            sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2022) Contact:Masahiro Suzuki Fujita Health
            University, School of Medicine, Microbiology; Dengakugakubo 1-98,
            Kutsukake-cho, Toyoake, Aichi 470-1192, Japan URL
            :http://www.fujita-hu.ac.jp/~microb/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9b
            Genome Coverage                   :: 272x
            Sequencing Technology             :: MiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_024182025.1-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 05:06:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,052
            Genes (coding)                    :: 4,941
            CDSs (with protein)               :: 4,941
            Genes (RNA)                       :: 113
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 45 of 111
            Pseudo Genes (incomplete)         :: 76 of 111
            Pseudo Genes (internal stop)      :: 12 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            Pseudo Genes (short protein)      :: 1 of 111
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP025846.1.
            Annotated by DFAST v.1.2.17 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="FUJ80379"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pFJMB80379_4"
                     /geo_loc_name="Japan:Aichi"
                     /lat_lon="35.07 N 137.00 E"
                     /collection_date="2020-04-25"
     gene            343..528
                     /locus_tag="NMC33_RS25755"
                     /old_locus_tag="FJMB80379_50640"
     CDS             343..528
                     /locus_tag="NMC33_RS25755"
                     /old_locus_tag="FJMB80379_50640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_013772937.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(819..1037)
                     /locus_tag="NMC33_RS25760"
     CDS             complement(819..1037)
                     /locus_tag="NMC33_RS25760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_198129632.1"
                     /translation="MFGFSSGALMTLSDDERHLLVSVVSVWLRRAGGDAGAMMLDAYRQ
                     ILSETEPAVRTVMLEFLESVRIHYISS"
                     /name="hypothetical protein"
     gene            2245..2472
                     /locus_tag="NMC33_RS25765"
                     /old_locus_tag="FJMB80379_50670"
     CDS             2245..2472
                     /locus_tag="NMC33_RS25765"
                     /old_locus_tag="FJMB80379_50670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     Typing          complement(576..663)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          1302..1830
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.598"
                     /coverage="88.0"
                     /evalue="0.0"
ORIGIN
        1 taatgtggcc tttttcttta cctgtggttc tccgccaaaa tcccagcaaa ttgcatcacc
       61 aaagctaaaa gctttcaggg ttgtctaaaa agcacaagac ataagaggaa gtgcggtatt
      121 ttataatcaa gcccccaaga atttaccata acatccgttg cccgcaccgc ctgagacgcg
      181 ttcagcgcgt tcctgacgaa accatgacaa aaaccacaac aaaccacccc ggaacccgtc
      241 agaaacgcgc ctgttaaatt ttaacggcat gcatgactat gcaccagaat gacgccatgc
      301 tcttttcacg caaaaatcat caccagacgg ggaaaatcac cagtgaccag acaggaatcc
      361 gccgccctca atatggccaa atttatccgc gcacagacac ttctcctcct tgagcggctc
      421 gagcagatgg atctggatga ggctgccggc tgctgtgagc acctgcacga tcaggccgaa
      481 gcgctttacg ccatgctgaa cgcacagata ggcgaggaaa atgcgtgaaa atcggtgaac
      541 gggtgcgcaa ttcagtgcgc ggccgtgagg cgatggcggg gtgtcggggc gcagccctga
      601 ccagggtatt tgtgatgccg gcgcgtgcgc ggtattacaa atgcacatcc tgtcccggaa
      661 cggacaccgg gaaacagcaa aaaaaaccgg gcggcacgcc cggaactcaa tcaagttaga
      721 ttagattact ctcactcgtc cataacagca tcatggaacg acgaccaccg tccgtgacgg
      781 ccgcctcgtt taagtatgga cagaaataca gaaaatgctc aggacgaaat gtaatgaatg
      841 cgaacggatt caagaaattc gagcatgaca gtccttacgg ccggttcggt ttcagacaaa
      901 atctgccggt atgcatccag catcatggct ccggcatccc ctccggcacg ccgtagccag
      961 accgaaacaa cggacacaag caggtgtcgc tcatcatcac taagagtcat cagggctccg
     1021 gaagaaaaac caaacatggc ttacctactg ttaacggcaa cgcagaagaa aggtttacag
     1081 ggtagcgggc agggatgaaa aaaaaaaaat tgcagtcagg ccggaacggc tgctgaaccg
     1141 gtacaggcgg gttccgggcg cagcgccgga ccagtcacgg agcactagcg gagtgtatac
     1201 tggcttaaga tgtagcatgg cagtaacaca tagcattaat gccacataag acaggcgcag
     1261 aaagtgaaag aaaacgcgtc aggagaaaat gtgatacaga atatattccg cttcctcgct
     1321 cactgactcg ctccgctcgg tcgttcggct gcggcgagcg ggacgggctt acggatggat
     1381 cagaaatttc ctgggggatg ccaggaagat acttaacgaa gaaagcataa ggccgcgcca
     1441 aagccgtttt tccataggct ccgcccccct gacaagcatc acagaatctg acgctcaaat
     1501 cagtggtggc gaaacccgat gcggcttaaa gataccaggc gtttccccct ggtggctccc
     1561 tcgtgctccc ccgcattcct gcctttcggt ttaccggtgt cactccgctg ttatggccgc
     1621 ggtttctcat tccacgcctg acactcagtt ccgggaaggc agttcgctcc aggatggact
     1681 gtatgcacga accccccgtt cagtccgacc gctgcgcctt atccggtaac tatcaacttg
     1741 agtccaaccc ggaaagacac gactaatcgc cattggcaac ggccattggc aaacaggatg
     1801 tgcgagagat gtctcaagag ttcttgaagt ggcggcctta gtgaggctac actgaaagga
     1861 caaatttggt gacatgtctc gcacaagact gttaccacgg ttaggggatc tgccaggatt
     1921 acctaacctt cgaaaaacca cctgccaggg tggttttttc gtttacaggg caagagatta
     1981 cgcgcggaaa aaaaggatct caagaagatc ctttataaat tagacatcca acctcacaaa
     2041 gaggaatact taaatcatct tggtatatgt atcggccgga ttacatctca tataagcctc
     2101 ctccccgcct gacaccacac tttgaccata gcacctcgcg cctaactcga tatacagccc
     2161 accatcaagc ctcttttcac ataaaaaatg acctatagtt agatggttag tgaccaaaac
     2221 tgatatggat aaaaggagct ggctttgaaa aagatttttg tttcagtgtt cgcagcggca
     2281 gttgcgcttt cagccctcac cggctgcacg cgcacaagct atgccataca caccaatgac
     2341 ggtcgcacta ttgtcagcga cggcaaacct aaagaatcag actcaggcct gcttggctat
     2401 acagacgcga atggtgtcaa acagcagatc aataaaaccg acgttaaaga agtctccgaa
     2461 attccgcact gagcatcttc tccgaaaaag gccgc
//
