LOCUS       NZ_AP025845             4781 bp    DNA     circular CON 22-APR-2024
DEFINITION  Enterobacter hormaechei strain FUJ80379 plasmid pFJMB80379_3,
            complete sequence.
ACCESSION   NZ_AP025845
VERSION     NZ_AP025845.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00490869
            Assembly: GCF_024182025.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Rapid phylogenetic analysis of Enterobacter hormaechei using open
            reading frame content patterns acquired by Oxford Nanopore
            sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4781)
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2022) Contact:Masahiro Suzuki Fujita Health
            University, School of Medicine, Microbiology; Dengakugakubo 1-98,
            Kutsukake-cho, Toyoake, Aichi 470-1192, Japan URL
            :http://www.fujita-hu.ac.jp/~microb/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9b
            Genome Coverage                   :: 272x
            Sequencing Technology             :: MiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_024182025.1-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 05:06:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,052
            Genes (coding)                    :: 4,941
            CDSs (with protein)               :: 4,941
            Genes (RNA)                       :: 113
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 45 of 111
            Pseudo Genes (incomplete)         :: 76 of 111
            Pseudo Genes (internal stop)      :: 12 of 111
            Pseudo Genes (multiple problems)  :: 20 of 111
            Pseudo Genes (short protein)      :: 1 of 111
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP025845.1.
            Annotated by DFAST v.1.2.17 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4781
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="FUJ80379"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pFJMB80379_3"
                     /geo_loc_name="Japan:Aichi"
                     /lat_lon="35.07 N 137.00 E"
                     /collection_date="2020-04-25"
     gene            join(4628..4781,1..191)
                     /locus_tag="NMC33_RS25725"
     CDS             join(4628..4781,1..191)
                     /locus_tag="NMC33_RS25725"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_126108277.1"
                     /translation="MSEKRNKMLTMWVTEDEHRRLLERCDGRQLAAWMRQICLDEKPAR
                     SGKLPSISPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHA
                     VLEKGTDDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            400..>831
                     /locus_tag="NMC33_RS25730"
                     /pseudo=""
     CDS             400..>831
                     /locus_tag="NMC33_RS25730"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            869..1360
                     /locus_tag="NMC33_RS25735"
                     /old_locus_tag="FJMB80379_50600"
     CDS             869..1360
                     /locus_tag="NMC33_RS25735"
                     /old_locus_tag="FJMB80379_50600"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_123924000.1"
                     /translation="MSSLLTLAKDLEQQSKAQKQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEVIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            1367..1582
                     /locus_tag="NMC33_RS25740"
                     /old_locus_tag="FJMB80379_50610"
     CDS             1367..1582
                     /locus_tag="NMC33_RS25740"
                     /old_locus_tag="FJMB80379_50610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            2325..3629
                     /locus_tag="NMC33_RS25745"
                     /old_locus_tag="FJMB80379_50630"
     CDS             2325..3629
                     /locus_tag="NMC33_RS25745"
                     /old_locus_tag="FJMB80379_50630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007899670.1"
                     /GO_process="GO:0006935 - chemotaxis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting chemotaxis
                     protein"
                     /protein_id="WP_063613192.1"
                     /translation="MRSLKNLFSLNRQRSAISYDAICQNKPVIEFSPEGVIHKASPLFL
                     STMGYRADEIIGHHHRIFCPPSLVSSPEYSHFWQRLARGESFSGKFLRRAKGNRSVWLE
                     ASYIPVTDRRGRVTRVIKIAADISERVHSALEQEAVLNAINRSMAIITFNPEGIVLDAN
                     ENFVNATGYKRDEVIGKHHRLFCAETLYKSDEYRQFWERLNQGEFFSGQFPRINRQGDP
                     LWFRATYNPVFNSDGQLYKIVKFATDVTADVLRNQREQEAAVHAWDMAVQTRGSAQNGA
                     NVIENSILMIDRIAQGMGAVSTDVSRLNNQSESIDGMVETIRKFAMQTRLIALNAAIEA
                     ARAGASGRSFAVVAAEVRNLAASVSSATEEIEQVVASNSQLAKDVLSGIENSLMTTREG
                     VTLMREAGEVMTSIQRNAAGVETAVKDVAISVKAG"
                     /name="PAS domain-containing methyl-accepting chemotaxis
                     protein"
     gene            complement(3853..3999)
                     /locus_tag="NMC33_RS25750"
     CDS             complement(3853..3999)
                     /locus_tag="NMC33_RS25750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063613191.1"
                     /translation="MHEPPVQSDRCALSGNYRLESNPERHDKTPLAAATGKRVYKRLLM
                     QSS"
                     /name="hypothetical protein"
     Typing          4361..4534
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.591"
                     /coverage="100.0"
                     /evalue="2.6e-76"
     Typing          complement(3831..4437)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.984"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4..185
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.443"
                     /coverage="98.0"
                     /evalue="1.19e-28"
     Typing          182..1674
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.6"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4650..4780
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.182"
                     /coverage="98.0"
                     /evalue="8.600000000000001e-21"
ORIGIN
        1 tctcgccggc gctgctgcgc cagcttgccg gcatgggcaa taacctgaac cagatagccc
       61 gccgtgttaa cgccggcggc ggcaccggcc atgacagggt acagattgtg gcggcgctga
      121 tggccatcga tgccgggctt gagcggctgc ggcacgccgt tcttgagaaa ggaacggacg
      181 atgatcgtta aatttcatcc acgcggtcga ggcggtggtg ccgggccggt ggactatctg
      241 ctgggtaaag accggcagcg tgaaggcgcc agcgtcctgc aggggaagcc ggaagaagtc
      301 cgggagctga ttgacgcgtc gccttacgtg aagaaataca cctccggcgt cctgtcgttt
      361 gcggaggccg atctgcctcc cggccagcgg gaaaaactga tggccagctt cgagcgggtt
      421 ctgatgcccg gacttgataa agatcagtac agcattttgt gggtggagca cgaggacaag
      481 gggcgtctgg agctgaactt tctgatcccc aataccgagc tgctgaccgg caggcggctc
      541 cagccgtact acgaccgcgc cgaccggccg cgtatcgatg catggcagac catcgtgaac
      601 ggcagactgg agctgcacga cccgaacgca ccggaaaacc gccgggcgct ggtcacgccg
      661 tccggactgc cgaaaacgaa gcaggaggcc gcagaagcca tcacgcgcgg actgctgacc
      721 cttgcttcgt ccggggagct gaaaacccgt caggacgtca ctgaggcgct ggaacggtca
      781 ggttttgagg tggtacgcac cacgaaaagc agtatcagca ttgccgaccc ggacgggggg
      841 cgaaacatcc gactgaaggg agccatctat gagcagtctt ttaacgctgg cgaaggactt
      901 ggagcagcaa tcgaaagcgc agaagcagag taccggcgag atgctgaaag ccgcattcag
      961 cgagcacgag cagtctgtca gagcggaact gagcgcaagc gcgaggagaa tcagcgacgc
     1021 catcctcgcc cacgagcaga gtatgagcga ggcgatggag aaaaaccggc ggagcgtatt
     1081 gcggactgcc gggcggacat ggctcaccat cctgatggtg tcagcgctgc tgatcggcac
     1141 gagcgggagc atactgtggt ggcaggggca gcagataacc gacaattaca cgcatcttcg
     1201 tcagcaggaa gataccctgg cgaaaatgac cgccaggacg tggggagtgc gctatcagga
     1261 gagcagcgac ggccggaggt ttcttatcct gccgccgggg atgcaaaccg aagtcatccc
     1321 ttacgacggg acgacatgga tccgcctgaa acaggagtag cggaagatga cagagctgga
     1381 aaagcagttg ctgagcgcat tagagcagct acagcaggac tactcgcaaa ggctggacga
     1441 atgggagagc gccttcgcgg aatggcggag catgtgtggt cttatgcaac gggagaacgc
     1501 ggtgctgaac gagcgcgtgt cggacttgag cacgcaggtg ctgagtttaa gcgagcagct
     1561 gcgccgcttg tccgggaact gaatgccacc gagttccggc agagggagaa tgagtatcag
     1621 aaaacgctcg agctgcagcg ccagccgcag aaaacctaca aaggaccgac gctgggctga
     1681 cagatgccgg cccgtgacaa accccaagaa gaagggctct tatctctccc gctaaatagg
     1741 ttcgtgacaa aaaaggtcaa ggcttattca ggagcgggaa atgaaattgc ttaacgttat
     1801 tgaagctgta cttaactgtc tgtatctggc tgcagagctg tgggagtggc tggagaagca
     1861 tttgccggtt tttaaggcat tggcgcggat tttagtgtga ctttaacatc catgcatctg
     1921 gctgcatgac tatgcaccag tgtaatattc agcggctgcg tttctggtgt tttttcgtta
     1981 tgtctgtcgc cactttctcg cgtctggcac cggaaacgca gccaaccccg tttcagtggt
     2041 gcgggcaacg gatgttatgg taaattcgtc aggctgttgc attaagtttc atgaacaatg
     2101 caacttaagc gcgtgacgaa aaaattaccg cctgccctct aaggccggcg cggcggcggc
     2161 cgataactga ggtgcctgaa accaggtttt aactctgtga cacgttgtct tccgctccct
     2221 gcccgggagc tttttttctg ccaccggcag ggcgggcatg atggtatccg cgtcctcggg
     2281 gataaccatg gcagtcctta cattatccga gcaatctgat gtatatgcgt tcacttaaaa
     2341 atctgttctc cctgaaccgc cagcgctcag ccatttcgta tgacgcaatt tgtcagaata
     2401 agcctgtcat tgaattcagt ccggaggggg tgatacataa agccagcccg ctttttctct
     2461 ccactatggg gtacagggct gatgaaataa tcggtcacca ccatcgtata ttctgcccac
     2521 cctctctggt gagttcaccg gaatacagcc atttctggca gcgccttgcc cggggagaaa
     2581 gctttagtgg caagttcctg cggcgggcaa agggaaatcg ttccgtatgg cttgaagcca
     2641 gttatatccc cgttactgac cggcgcggtc gtgtgaccag agtcattaaa attgctgcag
     2701 atatttctga gcgcgtgcat tctgcgcttg agcaggaagc agtcctaaat gccataaacc
     2761 gttctatggc cataatcacc ttcaatcctg aagggatcgt gcttgatgca aatgaaaatt
     2821 tcgtcaatgc cactggctat aaacgggatg aagtcatcgg taagcatcat cgtctgtttt
     2881 gtgccgagac gctttacaaa agtgatgaat accgacagtt ctgggagagg ctgaatcagg
     2941 gtgagttttt ttctggtcag tttccacgta ttaaccgtca gggagaccct ctgtggttcc
     3001 gtgcaactta taaccctgtc tttaacagtg acgggcagct ttataaaatt gtgaaattcg
     3061 ctacagatgt cacggctgat gtcctgcgaa accagagaga gcaggaggct gctgtgcatg
     3121 catgggatat ggccgtgcag acacgaggaa gtgcgcagaa cggtgcaaat gttattgaaa
     3181 atagcatcct tatgattgac agaattgcgc aggggatggg agctgtctcc accgatgtct
     3241 cccggctcaa caatcagtct gaaagtattg acggtatggt ggaaaccatc cggaagtttg
     3301 ccatgcaaac aagacttatt gcattgaatg ccgcgattga agcagcaagg gcgggcgcat
     3361 ccggaagaag ttttgcggtt gttgccgcag aggtgcgtaa tcttgcggcg agcgtcagta
     3421 gcgcgactga agaaattgag caagtcgtgg ccagcaatag tcaactggct aaagatgttc
     3481 tcagtggcat tgaaaacagc ctgatgacca cccgggaagg ggtcacactc atgcgtgaag
     3541 cgggtgaagt gatgaccagc attcagagga atgctgcagg agttgagact gcggttaagg
     3601 atgtcgccat ttcagttaaa gccggatagg ccatggtgca tatccccata tctgtcgaac
     3661 atcacagatg atcttcttga gatcctttcc gttgcgtcgt aatctcttgc tctgaaaacg
     3721 aaaaaaccac ccggcaaggt ggtttttcga aggttcatta aaccggcaag tttatgaacc
     3781 gtggtaacag ggtgtacaag accgctgcca ccaaaatcgt ccttccagtt tagccgtagt
     3841 tgggctgaga cttcaagaac tctgcatcag taatctcttg tacaccctct taccagtggc
     3901 tgctgccagt ggcgttttgt cgtgtctttc cgggttggac tcaagtcgat agttaccgga
     3961 taaggcgcag cggtcggact gaacgggggg ttcgtgcata cagtccagct tggagcgaac
     4021 tgccttcccg gaaccgagtg tcaggcgtgg aatgagaaac cgcggccata acagcggaat
     4081 gacaccggta aaccgaaagg caggaacagg agagcgcacg agggagccac cagggggaaa
     4141 cgcctggtat ctttatagtc ctgtcgggtt tcgccaccac tgatttgagc gtcagattct
     4201 gtgatgcttg tcaggggggc ggagcctatg gaaaaacggc ttcgccgcag ccttctctcc
     4261 gttccgctaa gtatcttcct ggcatcttct aggaattttc caccccaccg gtaagccggt
     4321 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggcatat
     4381 ctccgttatt acatattctg ctgacgcgcc ggtgccgcct tctttctcct gccacatgaa
     4441 gcacttcact gacatccgca tccgtgccaa catattaagc cagtatacac tccgctagcg
     4501 ctacgtgact ggttcagggc tgcgccccga aacccgctaa aagtcactga cgcgcctgcg
     4561 gcttgtccaa ccccgcgccg accgggaaag gctttcccgc ccgtcccggc gttatcagga
     4621 ggccggtttg tcagagaaac ggaacaagat gctcaccatg tgggtgacgg aggacgagca
     4681 ccggcggctg cttgagcgct gtgacggcag gcagctggcg gcgtggatgc ggcagatctg
     4741 cctggacgaa aagccggcac gttccggaaa gctcccctcg a
//
