LOCUS       NZ_AP025820             2496 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain FUJ80065 plasmid pFJMB80065_2,
            complete sequence.
ACCESSION   NZ_AP025820
VERSION     NZ_AP025820.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00490856
            Assembly: GCF_023735295.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Rapid phylogenetic analysis of Enterobacter hormaechei using open
            reading frame content patterns acquired by Oxford Nanopore
            sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2022) Contact:Masahiro Suzuki Fujita Health
            University, School of Medicine, Microbiology; Dengakugakubo 1-98,
            Kutsukake-cho, Toyoake, Aichi 470-1192, Japan URL
            :http://www.fujita-hu.ac.jp/~microb/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9b
            Genome Coverage                   :: 364x
            Sequencing Technology             :: MiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023735295.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 21:46:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,950
            CDSs (total)                      :: 4,835
            Genes (coding)                    :: 4,736
            CDSs (with protein)               :: 4,736
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 38 of 99
            Pseudo Genes (incomplete)         :: 69 of 99
            Pseudo Genes (internal stop)      :: 14 of 99
            Pseudo Genes (multiple problems)  :: 20 of 99
            Pseudo Genes (short protein)      :: 1 of 99
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP025820.1.
            Annotated by DFAST v.1.2.17 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="FUJ80065"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pFJMB80065_2"
                     /geo_loc_name="Japan:Aichi"
                     /lat_lon="35.07 N 137.00 E"
                     /collection_date="2014-01-08"
     gene            574..792
                     /locus_tag="NGJ50_RS24685"
                     /old_locus_tag="FJMB80065_48340"
     CDS             574..792
                     /locus_tag="NGJ50_RS24685"
                     /old_locus_tag="FJMB80065_48340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_182407767.1"
                     /translation="MFGFSSGALMTFNDDERHLLVSVVSGWLRRAEGDAGAMMLDAYRQ
                     ILSETEPAARAVMLEFLESVRIHYVSS"
                     /name="hypothetical protein"
     gene            complement(1090..1275)
                     /locus_tag="NGJ50_RS24690"
                     /old_locus_tag="FJMB80065_48360"
     CDS             complement(1090..1275)
                     /locus_tag="NGJ50_RS24690"
                     /old_locus_tag="FJMB80065_48360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032673404.1"
                     /translation="MTRQESAALNMAKFIRSQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEKDA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1641..1868)
                     /locus_tag="NGJ50_RS24695"
                     /old_locus_tag="FJMB80065_48370"
     CDS             complement(1641..1868)
                     /locus_tag="NGJ50_RS24695"
                     /old_locus_tag="FJMB80065_48370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     Typing          295..464
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.698"
                     /coverage="98.0"
                     /evalue="2.0600000000000002e-57"
     Typing          956..1043
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="94.382"
                     /coverage="100.0"
                     /evalue="1.5000000000000001e-30"
     Typing          complement(2..188)
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.889"
                     /coverage="84.0"
                     /evalue="4.39e-58"
     Typing          complement(2104..2496)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="82.41"
                     /coverage="99.0"
                     /evalue="1.12e-89"
ORIGIN
        1 ccataacagc ggagtgacac cggtaaaccg aaaggcagga atgcggggga gcacgaggga
       61 gccaccaggg ggaaacgcct ggtatcttta agccgcatcg ggtttcgcca ccactgattt
      121 gagcgtcaga tttcgtgatg cttgtcaggg gggcggagcc tatggaaaaa cggctttgcc
      181 gtggccttat gcctttttcg ctaagaacct tcctggcatt ctccaggaaa tttctgctct
      241 gtacgtaagc ccgtaccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
      301 aggaagcgga atatctcctg tatcacatat tctgctgacg caccgatgcc gcctttttct
      361 cctgccacat gaagcactta actgacatcc gcatccgtgc caacatatta agccagtata
      421 cactccgcta gcgctgcgtg actggtccgg cgctgcgccc ggaacccgcc tgtaccggtt
      481 aagcagccgt tctggtctga ctgcgatttt tttcattcct gcccgctacc ctgtaaacct
      541 ttcttctgcg ttgccgttaa caggaggtaa gccatgtttg gtttttcctc cggagccctg
      601 atgactttta atgatgatga gcgacacctg cttgtgtccg ttgtttcggg ctggctccgg
      661 cgtgccgaag gggatgccgg agccatgatg ctggatgcat accggcagat tttgtctgaa
      721 accgaaccgg ccgcaagggc ggtcatgctc gaatttcttg aatcagttcg cattcattac
      781 gtttcgtcct gagcatttcc tgaagcattt cctgaatttt tgtccatact caatccaggc
      841 ggccgtcacg gacggtggtc gtcgttccat gatgctgtta tggaagattg agagtaatct
      901 aacttgattg agttccgggc gtgtcgcccg gttttttttg ctgtttcccg gtgtccgctc
      961 ccggacagga tgtgcatttg taataccgcg cacgcggcgg catcacaaat accccgttca
     1021 gggctgcgcc ccgacacccc gccatcgcct cacggccgcg cactgaattg cgcacccgtt
     1081 caccgatttt cacgcatctt tctcgcctat ctgtgcgttc agcatggcgt aaagcgcttc
     1141 agcctgatcg tgcagatgct cacagcagcc ggctgcctca tccagatcca tctgttcgag
     1201 ccgttcgagg aggaggagtg tctgtgagcg gataaatttg gccatgttga gggcggcgga
     1261 ttcctgtctg gtcactggtg attttccccg tctggtgatg atttttgcgt gaaaagagca
     1321 tgccgtcatt ctggtgcata gtcatgcatg ccgttaaaat ttaacaggcg cgtttctgac
     1381 gggttccggg gtggtttgtt gtggtttttg tcatggttcc gtcagaaacg cgctgagcgc
     1441 gtctgaggcg gtgcgggcaa cggatgttat ggtaaattct tgggggcttg attataaaat
     1501 accgcacttc ctcttatgtc ttgtgctttt tagacaaccc tgaaagcttt tagctttggt
     1561 gatgcaattt gctgggattt tggcggagaa ccacaggtaa agaaaaaggc cacattagcg
     1621 gcctttttcg gagaatatgc tcagtgcgga atttcggaga cttctttaac gtcggtttta
     1681 ttgatctgct gtttgacacc attcgcgtct gtatagccaa gcaggcctga gtctgattct
     1741 ttaggtttgc cgtcgctgac aatagtgcga ccgtcattgg tgtgtatggc atagcttgtg
     1801 cgcgtgcagc cggtgagggc tgaaagcgca actgccgctg cgaacactga aacaaaaatc
     1861 tttttcaaag ccagctcctt ttatccatat cagttttggt cactaaccat ctaactatag
     1921 gttatttttt atgtgaaaag aggcttgatg gtgggctgta tatcgagtta ggcgcgaggt
     1981 gctatggtca aagtgtggtg tcaagcgggg aggaggctta tatgagatgt aatccagcag
     2041 atacatatac caagatgatt taagtattcc tctttgtgag gttggatgtc taatttataa
     2101 aggatcttct tgagatcctt ttttttcgcg cgtaatctct tgccctgtaa acgaaaaaac
     2161 caccctggca ggtggttttt cgaaggttag gtaatcctgg cagatcccct aaccgtggta
     2221 acagtcttgt gcgagacatg tcaccaaatt tgtcctttca gtgtagcctc actaaggccg
     2281 ccacttcaag aactcttgat acatctctcg cacatcctgt ttaccaatgg ccgttgccaa
     2341 tggcgattag tcgtgtcttt ccgggttgga ctcaagttga tagttaccgg ataaggcgca
     2401 gcggtcggac tgaacggggg gttcgtgcat acagtccatc ctggagcgaa ctgccttccc
     2461 ggaactgagt gtcaggcgtg gaatgagaaa ccgcgg
//
