LOCUS       NZ_AP025817             2496 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain FUJ80064 plasmid pFJMB80064_2,
            complete sequence.
ACCESSION   NZ_AP025817
VERSION     NZ_AP025817.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00490855
            Assembly: GCF_023735275.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Rapid phylogenetic analysis of Enterobacter hormaechei using open
            reading frame content patterns acquired by Oxford Nanopore
            sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2022) Contact:Masahiro Suzuki Fujita Health
            University, School of Medicine, Microbiology; Dengakugakubo 1-98,
            Kutsukake-cho, Toyoake, Aichi 470-1192, Japan URL
            :http://www.fujita-hu.ac.jp/~microb/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9b
            Genome Coverage                   :: 359x
            Sequencing Technology             :: MiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023735275.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 21:45:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,962
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,747
            CDSs (with protein)               :: 4,747
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 38 of 100
            Pseudo Genes (incomplete)         :: 70 of 100
            Pseudo Genes (internal stop)      :: 14 of 100
            Pseudo Genes (multiple problems)  :: 20 of 100
            Pseudo Genes (short protein)      :: 1 of 100
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP025817.1.
            Annotated by DFAST v.1.2.17 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="FUJ80064"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pFJMB80064_2"
                     /geo_loc_name="Japan:Aichi"
                     /lat_lon="35.07 N 137.00 E"
                     /collection_date="2014-09-12"
     gene            complement(join(2373..2496,1..104))
                     /locus_tag="NGJ20_RS24745"
     CDS             complement(join(2373..2496,1..104))
                     /locus_tag="NGJ20_RS24745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            1306..1524
                     /locus_tag="NGJ20_RS24750"
                     /old_locus_tag="FJMB80064_48420"
     CDS             1306..1524
                     /locus_tag="NGJ20_RS24750"
                     /old_locus_tag="FJMB80064_48420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_182407767.1"
                     /translation="MFGFSSGALMTFNDDERHLLVSVVSGWLRRAEGDAGAMMLDAYRQ
                     ILSETEPAARAVMLEFLESVRIHYVSS"
                     /name="hypothetical protein"
     gene            complement(1822..2007)
                     /locus_tag="NGJ20_RS24755"
                     /old_locus_tag="FJMB80064_48430"
     CDS             complement(1822..2007)
                     /locus_tag="NGJ20_RS24755"
                     /old_locus_tag="FJMB80064_48430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032673404.1"
                     /translation="MTRQESAALNMAKFIRSQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEKDA"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          1027..1196
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.698"
                     /coverage="98.0"
                     /evalue="2.0600000000000002e-57"
     Typing          1688..1775
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="94.382"
                     /coverage="100.0"
                     /evalue="1.5000000000000001e-30"
     Typing          complement(520..1102)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.331"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 gtgcgaccgt cattggtgtg tatggcatag cttgtgcgcg tgcagccggt gagggctgaa
       61 agcgcaactg ccgctgcgaa cactgaaaca aaaatctttt tcaaagccag ctccttttat
      121 ccatatcagt tttggtcact aaccatctaa ctataggtta ttttttatgt gaaaagaggc
      181 ttgatggtgg gctgtatatc gagttaggcg cgaggtgcta tggtcaaagt gtggtgtcaa
      241 gcggggagga ggcttatatg agatgtaatc cagcagatac atataccaag atgatttaag
      301 tattcctctt tgtgaggttg gatgtctaat ttataaagga tcttcttgag atcctttttt
      361 ttcgcgcgta atctcttgcc ctgtaaacga aaaaaccacc ctggcaggtg gtttttcgaa
      421 ggttaggtaa tcctggcaga tcccctaacc gtggtaacag tcttgtgcga gacatgtcac
      481 caaatttgtc ctttcagtgt agcctcacta aggccgccac ttcaagaact cttgatacat
      541 ctctcgcaca tcctgtttac caatggccgt tgccaatggc gattagtcgt gtctttccgg
      601 gttggactca agttgatagt taccggataa ggcgcagcgg tcggactgaa cggggggttc
      661 gtgcatacag tccatcctgg agcgaactgc cttcccggaa ctgagtgtca ggcgtggaat
      721 gagaaaccgc ggccataaca gcggagtgac accggtaaac cgaaaggcag gaatgcgggg
      781 gagcacgagg gagccaccag ggggaaacgc ctggtatctt taagccgcat cgggtttcgc
      841 caccactgat ttgagcgtca gatttcgtga tgcttgtcag gggggcggag cctatggaaa
      901 aacggctttg ccgtggcctt atgccttttt cgctaagaac cttcctggca ttctccagga
      961 aatttctgct ctgtacgtaa gcccgtaccg ctcgccgcag ccgaacgacc gagcgcagcg
     1021 agtcagtgag cgaggaagcg gaatatctcc tgtatcacat attctgctga cgcaccgatg
     1081 ccgccttttt ctcctgccac atgaagcact taactgacat ccgcatccgt gccaacatat
     1141 taagccagta tacactccgc tagcgctgcg tgactggtcc ggcgctgcgc ccggaacccg
     1201 cctgtaccgg ttaagcagcc gttctggtct gactgcgatt tttttcattc ctgcccgcta
     1261 ccctgtaaac ctttcttctg cgttgccgtt aacaggaggt aagccatgtt tggtttttcc
     1321 tccggagccc tgatgacttt taatgatgat gagcgacacc tgcttgtgtc cgttgtttcg
     1381 ggctggctcc ggcgtgccga aggggatgcc ggagccatga tgctggatgc ataccggcag
     1441 attttgtctg aaaccgaacc ggccgcaagg gcggtcatgc tcgaatttct tgaatcagtt
     1501 cgcattcatt acgtttcgtc ctgagcattt cctgaagcat ttcctgaatt tttgtccata
     1561 ctcaatccag gcggccgtca cggacggtgg tcgtcgttcc atgatgctgt tatggaagat
     1621 tgagagtaat ctaacttgat tgagttccgg gcgtgtcgcc cggttttttt tgctgtttcc
     1681 cggtgtccgc tcccggacag gatgtgcatt tgtaataccg cgcacgcggc ggcatcacaa
     1741 ataccccgtt cagggctgcg ccccgacacc ccgccatcgc ctcacggccg cgcactgaat
     1801 tgcgcacccg ttcaccgatt ttcacgcatc tttctcgcct atctgtgcgt tcagcatggc
     1861 gtaaagcgct tcagcctgat cgtgcagatg ctcacagcag ccggctgcct catccagatc
     1921 catctgttcg agccgttcga ggaggaggag tgtctgtgag cggataaatt tggccatgtt
     1981 gagggcggcg gattcctgtc tggtcactgg tgattttccc cgtctggtga tgatttttgc
     2041 gtgaaaagag catgccgtca ttctggtgca tagtcatgca tgccgttaaa atttaacagg
     2101 cgcgtttctg acgggttccg gggtggtttg ttgtggtttt tgtcatggtt ccgtcagaaa
     2161 cgcgctgagc gcgtctgagg cggtgcgggc aacggatgtt atggtaaatt cttgggggct
     2221 tgattataaa ataccgcact tcctcttatg tcttgtgctt tttagacaac cctgaaagct
     2281 tttagctttg gtgatgcaat ttgctgggat tttggcggag aaccacaggt aaagaaaaag
     2341 gccacattag cggccttttt cggagaatat gctcagtgcg gaatttcgga gacttcttta
     2401 acgtcggttt tattgatctg ctgtttgaca ccattcgcgt ctgtatagcc aagcaggcct
     2461 gagtctgatt ctttaggttt gccgtcgctg acaata
//
