LOCUS       NZ_AP025809             2496 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain FUJ80056 plasmid pFJMB80056_2,
            complete sequence.
ACCESSION   NZ_AP025809
VERSION     NZ_AP025809.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00490850
            Assembly: GCF_023735215.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Rapid phylogenetic analysis of Enterobacter hormaechei using open
            reading frame content patterns acquired by Oxford Nanopore
            sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2022) Contact:Masahiro Suzuki Fujita Health
            University, School of Medicine, Microbiology; Dengakugakubo 1-98,
            Kutsukake-cho, Toyoake, Aichi 470-1192, Japan URL
            :http://www.fujita-hu.ac.jp/~microb/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9b
            Genome Coverage                   :: 475x
            Sequencing Technology             :: MiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023735215.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 21:38:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,962
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,745
            CDSs (with protein)               :: 4,745
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 40 of 102
            Pseudo Genes (incomplete)         :: 70 of 102
            Pseudo Genes (internal stop)      :: 14 of 102
            Pseudo Genes (multiple problems)  :: 20 of 102
            Pseudo Genes (short protein)      :: 1 of 102
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP025809.1.
            Annotated by DFAST v.1.2.17 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="FUJ80056"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pFJMB80056_2"
                     /geo_loc_name="Japan:Aichi"
                     /lat_lon="35.07 N 137.00 E"
                     /collection_date="2015-11-11"
     gene            complement(join(2442..2496,1..164))
                     /locus_tag="NGK22_RS24745"
     CDS             complement(join(2442..2496,1..164))
                     /locus_tag="NGK22_RS24745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_182407767.1"
                     /translation="MFGFSSGALMTFNDDERHLLVSVVSGWLRRAEGDAGAMMLDAYRQ
                     ILSETEPAARAVMLEFLESVRIHYVSS"
                     /name="hypothetical protein"
     gene            1366..1593
                     /locus_tag="NGK22_RS24750"
                     /old_locus_tag="FJMB80056_48750"
     CDS             1366..1593
                     /locus_tag="NGK22_RS24750"
                     /old_locus_tag="FJMB80056_48750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            1959..2144
                     /locus_tag="NGK22_RS24755"
                     /old_locus_tag="FJMB80056_48760"
     CDS             1959..2144
                     /locus_tag="NGK22_RS24755"
                     /old_locus_tag="FJMB80056_48760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032673404.1"
                     /translation="MTRQESAALNMAKFIRSQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEKDA"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(272..444)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.286"
                     /coverage="100.0"
                     /evalue="2.0600000000000002e-57"
     Typing          complement(2192..2279)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="94.382"
                     /coverage="100.0"
                     /evalue="1.5000000000000001e-30"
     Typing          369..951
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.331"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 cttgcggccg gttcggtttc agacaaaatc tgccggtatg catccagcat catggctccg
       61 gcatcccctt cggcacgccg gagccagccc gaaacaacgg acacaagcag gtgtcgctca
      121 tcatcattaa aagtcatcag ggctccggag gaaaaaccaa acatggctta cctcctgtta
      181 acggcaacgc agaagaaagg tttacagggt agcgggcagg aatgaaaaaa atcgcagtca
      241 gaccagaacg gctgcttaac cggtacaggc gggttccggg cgcagcgccg gaccagtcac
      301 gcagcgctag cggagtgtat actggcttaa tatgttggca cggatgcgga tgtcagttaa
      361 gtgcttcatg tggcaggaga aaaaggcggc atcggtgcgt cagcagaata tgtgatacag
      421 gagatattcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
      481 ggtacgggct tacgtacaga gcagaaattt cctggagaat gccaggaagg ttcttagcga
      541 aaaaggcata aggccacggc aaagccgttt ttccataggc tccgcccccc tgacaagcat
      601 cacgaaatct gacgctcaaa tcagtggtgg cgaaacccga tgcggcttaa agataccagg
      661 cgtttccccc tggtggctcc ctcgtgctcc cccgcattcc tgcctttcgg tttaccggtg
      721 tcactccgct gttatggccg cggtttctca ttccacgcct gacactcagt tccgggaagg
      781 cagttcgctc caggatggac tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct
      841 tatccggtaa ctatcaactt gagtccaacc cggaaagaca cgactaatcg ccattggcaa
      901 cggccattgg taaacaggat gtgcgagaga tgtatcaaga gttcttgaag tggcggcctt
      961 agtgaggcta cactgaaagg acaaatttgg tgacatgtct cgcacaagac tgttaccacg
     1021 gttaggggat ctgccaggat tacctaacct tcgaaaaacc acctgccagg gtggtttttt
     1081 cgtttacagg gcaagagatt acgcgcgaaa aaaaaggatc tcaagaagat cctttataaa
     1141 ttagacatcc aacctcacaa agaggaatac ttaaatcatc ttggtatatg tatctgctgg
     1201 attacatctc atataagcct cctccccgct tgacaccaca ctttgaccat agcacctcgc
     1261 gcctaactcg atatacagcc caccatcaag cctcttttca cataaaaaat aacctatagt
     1321 tagatggtta gtgaccaaaa ctgatatgga taaaaggagc tggctttgaa aaagattttt
     1381 gtttcagtgt tcgcagcggc agttgcgctt tcagccctca ccggctgcac gcgcacaagc
     1441 tatgccatac acaccaatga cggtcgcact attgtcagcg acggcaaacc taaagaatca
     1501 gactcaggcc tgcttggcta tacagacgcg aatggtgtca aacagcagat caataaaacc
     1561 gacgttaaag aagtctccga aattccgcac tgagcatatt ctccgaaaaa ggccgctaat
     1621 gtggcctttt tctttacctg tggttctccg ccaaaatccc agcaaattgc atcaccaaag
     1681 ctaaaagctt tcagggttgt ctaaaaagca caagacataa gaggaagtgc ggtattttat
     1741 aatcaagccc ccaagaattt accataacat ccgttgcccg caccgcctca gacgcgctca
     1801 gcgcgtttct gacggaacca tgacaaaaac cacaacaaac caccccggaa cccgtcagaa
     1861 acgcgcctgt taaattttaa cggcatgcat gactatgcac cagaatgacg gcatgctctt
     1921 ttcacgcaaa aatcatcacc agacggggaa aatcaccagt gaccagacag gaatccgccg
     1981 ccctcaacat ggccaaattt atccgctcac agacactcct cctcctcgaa cggctcgaac
     2041 agatggatct ggatgaggca gccggctgct gtgagcatct gcacgatcag gctgaagcgc
     2101 tttacgccat gctgaacgca cagataggcg agaaagatgc gtgaaaatcg gtgaacgggt
     2161 gcgcaattca gtgcgcggcc gtgaggcgat ggcggggtgt cggggcgcag ccctgaacgg
     2221 ggtatttgtg atgccgccgc gtgcgcggta ttacaaatgc acatcctgtc cgggagcgga
     2281 caccgggaaa cagcaaaaaa aaccgggcga cacgcccgga actcaatcaa gttagattac
     2341 tctcaatctt ccataacagc atcatggaac gacgaccacc gtccgtgacg gccgcctgga
     2401 ttgagtatgg acaaaaattc aggaaatgct tcaggaaatg ctcaggacga aacgtaatga
     2461 atgcgaactg attcaagaaa ttcgagcatg accgcc
//
