LOCUS       NZ_AP025803             2496 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain FUJ80024 plasmid pFJMB80024_2,
            complete sequence.
ACCESSION   NZ_AP025803
VERSION     NZ_AP025803.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00490846
            Assembly: GCF_023735155.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Rapid phylogenetic analysis of Enterobacter hormaechei using open
            reading frame content patterns acquired by Oxford Nanopore
            sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2022) Contact:Masahiro Suzuki Fujita Health
            University, School of Medicine, Microbiology; Dengakugakubo 1-98,
            Kutsukake-cho, Toyoake, Aichi 470-1192, Japan URL
            :http://www.fujita-hu.ac.jp/~microb/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9b
            Genome Coverage                   :: 326x
            Sequencing Technology             :: MiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023735155.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 21:43:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,962
            CDSs (total)                      :: 4,847
            Genes (coding)                    :: 4,746
            CDSs (with protein)               :: 4,746
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 39 of 101
            Pseudo Genes (incomplete)         :: 70 of 101
            Pseudo Genes (internal stop)      :: 14 of 101
            Pseudo Genes (multiple problems)  :: 20 of 101
            Pseudo Genes (short protein)      :: 1 of 101
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP025803.1.
            Annotated by DFAST v.1.2.17 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="FUJ80024"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pFJMB80024_2"
                     /geo_loc_name="Japan:Aichi"
                     /lat_lon="35.07 N 137.00 E"
                     /collection_date="2015-11-11"
     gene            573..791
                     /locus_tag="NGJ01_RS24745"
                     /old_locus_tag="FJMB80024_48750"
     CDS             573..791
                     /locus_tag="NGJ01_RS24745"
                     /old_locus_tag="FJMB80024_48750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_182407767.1"
                     /translation="MFGFSSGALMTFNDDERHLLVSVVSGWLRRAEGDAGAMMLDAYRQ
                     ILSETEPAARAVMLEFLESVRIHYVSS"
                     /name="hypothetical protein"
     gene            complement(1089..1274)
                     /locus_tag="NGJ01_RS24750"
                     /old_locus_tag="FJMB80024_48770"
     CDS             complement(1089..1274)
                     /locus_tag="NGJ01_RS24750"
                     /old_locus_tag="FJMB80024_48770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032673404.1"
                     /translation="MTRQESAALNMAKFIRSQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEKDA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1640..1867)
                     /locus_tag="NGJ01_RS24755"
                     /old_locus_tag="FJMB80024_48780"
     CDS             complement(1640..1867)
                     /locus_tag="NGJ01_RS24755"
                     /old_locus_tag="FJMB80024_48780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     Typing          294..463
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.698"
                     /coverage="98.0"
                     /evalue="2.0600000000000002e-57"
     Typing          955..1042
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="94.382"
                     /coverage="100.0"
                     /evalue="1.5000000000000001e-30"
     Typing          complement(2..187)
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.83"
                     /coverage="84.0"
                     /evalue="1.5800000000000001e-57"
     Typing          complement(2103..2496)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="82.452"
                     /coverage="99.0"
                     /evalue="3.12e-90"
ORIGIN
        1 cataacagcg gagtgacacc ggtaaaccga aaggcaggaa tgcgggggag cacgagggag
       61 ccaccagggg gaaacgcctg gtatctttaa gccgcatcgg gtttcgccac cactgatttg
      121 agcgtcagat ttcgtgatgc ttgtcagggg ggcggagcct atggaaaaac ggctttgccg
      181 tggccttatg cctttttcgc taagaacctt cctggcattc tccaggaaat ttctgctctg
      241 tacgtaagcc cgtaccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
      301 ggaagcggaa tatctcctgt atcacatatt ctgctgacgc accgatgccg cctttttctc
      361 ctgccacatg aagcacttaa ctgacatccg catccgtgcc aacatattaa gccagtatac
      421 actccgctag cgctgcgtga ctggtccggc gctgcgcccg gaacccgcct gtaccggtta
      481 agcagccgtt ctggtctgac tgcgattttt ttcattcctg cccgctaccc tgtaaacctt
      541 tcttctgcgt tgccgttaac aggaggtaag ccatgtttgg tttttcctcc ggagccctga
      601 tgacttttaa tgatgatgag cgacacctgc ttgtgtccgt tgtttcgggc tggctccggc
      661 gtgccgaagg ggatgccgga gccatgatgc tggatgcata ccggcagatt ttgtctgaaa
      721 ccgaaccggc cgcaagggcg gtcatgctcg aatttcttga atcagttcgc attcattacg
      781 tttcgtcctg agcatttcct gaagcatttc ctgaattttt gtccatactc aatccaggcg
      841 gccgtcacgg acggtggtcg tcgttccatg atgctgttat ggaagattga gagtaatcta
      901 acttgattga gttccgggcg tgtcgcccgg ttttttttgc tgtttcccgg tgtccgctcc
      961 cggacaggat gtgcatttgt aataccgcgc acgcggcggc atcacaaata ccccgttcag
     1021 ggctgcgccc cgacaccccg ccatcgcctc acggccgcgc actgaattgc gcacccgttc
     1081 accgattttc acgcatcttt ctcgcctatc tgtgcgttca gcatggcgta aagcgcttca
     1141 gcctgatcgt gcagatgctc acagcagccg gctgcctcat ccagatccat ctgttcgagc
     1201 cgttcgagga ggaggagtgt ctgtgagcgg ataaatttgg ccatgttgag ggcggcggat
     1261 tcctgtctgg tcactggtga ttttccccgt ctggtgatga tttttgcgtg aaaagagcat
     1321 gccgtcattc tggtgcatag tcatgcatgc cgttaaaatt taacaggcgc gtttctgacg
     1381 ggttccgggg tggtttgttg tggtttttgt catggttccg tcagaaacgc gctgagcgcg
     1441 tctgaggcgg tgcgggcaac ggatgttatg gtaaattctt gggggcttga ttataaaata
     1501 ccgcacttcc tcttatgtct tgtgcttttt agacaaccct gaaagctttt agctttggtg
     1561 atgcaatttg ctgggatttt ggcggagaac cacaggtaaa gaaaaaggcc acattagcgg
     1621 cctttttcgg agaatatgct cagtgcggaa tttcggagac ttctttaacg tcggttttat
     1681 tgatctgctg tttgacacca ttcgcgtctg tatagccaag caggcctgag tctgattctt
     1741 taggtttgcc gtcgctgaca atagtgcgac cgtcattggt gtgtatggca tagcttgtgc
     1801 gcgtgcagcc ggtgagggct gaaagcgcaa ctgccgctgc gaacactgaa acaaaaatct
     1861 ttttcaaagc cagctccttt tatccatatc agttttggtc actaaccatc taactatagg
     1921 ttatttttta tgtgaaaaga ggcttgatgg tgggctgtat atcgagttag gcgcgaggtg
     1981 ctatggtcaa agtgtggtgt caagcgggga ggaggcttat atgagatgta atccagcaga
     2041 tacatatacc aagatgattt aagtattcct ctttgtgagg ttggatgtct aatttataaa
     2101 ggatcttctt gagatccttt tttttcgcgc gtaatctctt gccctgtaaa cgaaaaaacc
     2161 accctggcag gtggtttttc gaaggttagg taatcctggc agatccccta accgtggtaa
     2221 cagtcttgtg cgagacatgt caccaaattt gtcctttcag tgtagcctca ctaaggccgc
     2281 cacttcaaga actcttgata catctctcgc acatcctgtt taccaatggc cgttgccaat
     2341 ggcgattagt cgtgtctttc cgggttggac tcaagttgat agttaccgga taaggcgcag
     2401 cggtcggact gaacgggggg ttcgtgcata cagtccatcc tggagcgaac tgccttcccg
     2461 gaactgagtg tcaggcgtgg aatgagaaac cgcggc
//
