LOCUS       NZ_AP025786             2496 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain FUJ80012 plasmid pFJMB80012_2,
            complete sequence.
ACCESSION   NZ_AP025786
VERSION     NZ_AP025786.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00490838
            Assembly: GCF_023734955.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Rapid phylogenetic analysis of Enterobacter hormaechei using open
            reading frame content patterns acquired by Oxford Nanopore
            sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Hayashi,K., Doi,Y. and Suzuki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2022) Contact:Masahiro Suzuki Fujita Health
            University, School of Medicine, Microbiology; Dengakugakubo 1-98,
            Kutsukake-cho, Toyoake, Aichi 470-1192, Japan URL
            :http://www.fujita-hu.ac.jp/~microb/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9b
            Genome Coverage                   :: 76x
            Sequencing Technology             :: MiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023734955.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 21:40:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,966
            CDSs (total)                      :: 4,852
            Genes (coding)                    :: 4,750
            CDSs (with protein)               :: 4,750
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 39 of 102
            Pseudo Genes (incomplete)         :: 71 of 102
            Pseudo Genes (internal stop)      :: 14 of 102
            Pseudo Genes (multiple problems)  :: 20 of 102
            Pseudo Genes (short protein)      :: 1 of 102
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP025786.1.
            Annotated by DFAST v.1.2.17 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="FUJ80012"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pFJMB80012_2"
                     /geo_loc_name="Japan:Aichi"
                     /lat_lon="35.07 N 137.00 E"
                     /collection_date="2016-10-25"
     gene            join(2346..2496,1..68)
                     /locus_tag="NGJ22_RS24760"
     CDS             join(2346..2496,1..68)
                     /locus_tag="NGJ22_RS24760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_182407767.1"
                     /translation="MFGFSSGALMTFNDDERHLLVSVVSGWLRRAEGDAGAMMLDAYRQ
                     ILSETEPAARAVMLEFLESVRIHYVSS"
                     /name="hypothetical protein"
     gene            complement(366..551)
                     /locus_tag="NGJ22_RS24765"
                     /old_locus_tag="FJMB80012_48710"
     CDS             complement(366..551)
                     /locus_tag="NGJ22_RS24765"
                     /old_locus_tag="FJMB80012_48710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032673404.1"
                     /translation="MTRQESAALNMAKFIRSQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEKDA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(917..1144)
                     /locus_tag="NGJ22_RS24770"
                     /old_locus_tag="FJMB80012_48720"
     CDS             complement(917..1144)
                     /locus_tag="NGJ22_RS24770"
                     /old_locus_tag="FJMB80012_48720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     Typing          232..319
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="94.382"
                     /coverage="100.0"
                     /evalue="1.5000000000000001e-30"
     Typing          2067..2236
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.698"
                     /coverage="98.0"
                     /evalue="2.0600000000000002e-57"
     Typing          complement(1560..2142)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.331"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 aaccggccgc aagggcggtc atgctcgaat ttcttgaatc agttcgcatt cattacgttt
       61 cgtcctgagc atttcctgaa gcatttcctg aatttttgtc catactcaat ccaggcggcc
      121 gtcacggacg gtggtcgtcg ttccatgatg ctgttatgga agattgagag taatctaact
      181 tgattgagtt ccgggcgtgt cgcccggttt tttttgctgt ttcccggtgt ccgctcccgg
      241 acaggatgtg catttgtaat accgcgcacg cggcggcatc acaaataccc cgttcagggc
      301 tgcgccccga caccccgcca tcgcctcacg gccgcgcact gaattgcgca cccgttcacc
      361 gattttcacg catctttctc gcctatctgt gcgttcagca tggcgtaaag cgcttcagcc
      421 tgatcgtgca gatgctcaca gcagccggct gcctcatcca gatccatctg ttcgagccgt
      481 tcgaggagga ggagtgtctg tgagcggata aatttggcca tgttgagggc ggcggattcc
      541 tgtctggtca ctggtgattt tccccgtctg gtgatgattt ttgcgtgaaa agagcatgcc
      601 gtcattctgg tgcatagtca tgcatgccgt taaaatttaa caggcgcgtt tctgacgggt
      661 tccggggtgg tttgttgtgg tttttgtcat ggttccgtca gaaacgcgct gagcgcgtct
      721 gaggcggtgc gggcaacgga tgttatggta aattcttggg ggcttgatta taaaataccg
      781 cacttcctct tatgtcttgt gctttttaga caaccctgaa agcttttagc tttggtgatg
      841 caatttgctg ggattttggc ggagaaccac aggtaaagaa aaaggccaca ttagcggcct
      901 ttttcggaga atatgctcag tgcggaattt cggagacttc tttaacgtcg gttttattga
      961 tctgctgttt gacaccattc gcgtctgtat agccaagcag gcctgagtct gattctttag
     1021 gtttgccgtc gctgacaata gtgcgaccgt cattggtgtg tatggcatag cttgtgcgcg
     1081 tgcagccggt gagggctgaa agcgcaactg ccgctgcgaa cactgaaaca aaaatctttt
     1141 tcaaagccag ctccttttat ccatatcagt tttggtcact aaccatctaa ctataggtta
     1201 ttttttatgt gaaaagaggc ttgatggtgg gctgtatatc gagttaggcg cgaggtgcta
     1261 tggtcaaagt gtggtgtcaa gcggggagga ggcttatatg agatgtaatc cagcagatac
     1321 atataccaag atgatttaag tattcctctt tgtgaggttg gatgtctaat ttataaagga
     1381 tcttcttgag atcctttttt ttcgcgcgta atctcttgcc ctgtaaacga aaaaaccacc
     1441 ctggcaggtg gtttttcgaa ggttaggtaa tcctggcaga tcccctaacc gtggtaacag
     1501 tcttgtgcga gacatgtcac caaatttgtc ctttcagtgt agcctcacta aggccgccac
     1561 ttcaagaact cttgatacat ctctcgcaca tcctgtttac caatggccgt tgccaatggc
     1621 gattagtcgt gtctttccgg gttggactca agttgatagt taccggataa ggcgcagcgg
     1681 tcggactgaa cggggggttc gtgcatacag tccatcctgg agcgaactgc cttcccggaa
     1741 ctgagtgtca ggcgtggaat gagaaaccgc ggccataaca gcggagtgac accggtaaac
     1801 cgaaaggcag gaatgcgggg gagcacgagg gagccaccag ggggaaacgc ctggtatctt
     1861 taagccgcat cgggtttcgc caccactgat ttgagcgtca gatttcgtga tgcttgtcag
     1921 gggggcggag cctatggaaa aacggctttg ccgtggcctt atgccttttt cgctaagaac
     1981 cttcctggca ttctccagga aatttctgct ctgtacgtaa gcccgtaccg ctcgccgcag
     2041 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaatatctcc tgtatcacat
     2101 attctgctga cgcaccgatg ccgccttttt ctcctgccac atgaagcact taactgacat
     2161 ccgcatccgt gccaacatat taagccagta tacactccgc tagcgctgcg tgactggtcc
     2221 ggcgctgcgc ccggaacccg cctgtaccgg ttaagcagcc gttctggtct gactgcgatt
     2281 tttttcattc ctgcccgcta ccctgtaaac ctttcttctg cgttgccgtt aacaggaggt
     2341 aagccatgtt tggtttttcc tccggagccc tgatgacttt taatgatgat gagcgacacc
     2401 tgcttgtgtc cgttgtttcg ggctggctcc ggcgtgccga aggggatgcc ggagccatga
     2461 tgctggatgc ataccggcag attttgtctg aaaccg
//
