LOCUS       NZ_AP025757             6965 bp    DNA     circular CON 24-JUN-2023
DEFINITION  Escherichia sp. KS167_9B strain KS-P079 plasmid pla3, complete
            sequence.
ACCESSION   NZ_AP025757
VERSION     NZ_AP025757.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00159532
            Sequence Read Archive: DRR168433
            Assembly: GCF_030295215.1
KEYWORDS    RefSeq.
SOURCE      Escherichia sp. KS167_9B
  ORGANISM  Escherichia sp. KS167_9B
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Ogura,Y. and Okuno,M.
  TITLE     Escherichia cryptic clade I strains
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6965)
  AUTHORS   Ogura,Y. and Okuno,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAY-2022) Contact:Yoshitoshi Ogura Kurume University
            School of Medicine, Department of Infectious Medicine; Asahi-machi
            67, Kurume, Fukuoka 830-0011, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.4
            Genome Coverage                   :: 117x
            Sequencing Technology             :: MinION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/22/2023 11:55:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,675
            CDSs (total)                      :: 5,544
            Genes (coding)                    :: 5,294
            CDSs (with protein)               :: 5,294
            Genes (RNA)                       :: 131
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 100
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 104 of 250
            Pseudo Genes (incomplete)         :: 164 of 250
            Pseudo Genes (internal stop)      :: 45 of 250
            Pseudo Genes (multiple problems)  :: 59 of 250
            Pseudo Genes (short protein)      :: 3 of 250
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP025757.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6965
                     /organism="Escherichia sp. KS167_9B"
                     /mol_type="genomic DNA"
                     /strain="KS-P079"
                     /isolation_source="feces"
                     /host="Bos taurus"
                     /db_xref="taxon:2509666"
                     /plasmid="pla3"
                     /geo_loc_name="Japan"
                     /collection_date="2013"
     gene            complement(28..219)
                     /locus_tag="QUE34_RS28310"
                     /old_locus_tag="EsCd1KSP079_05615"
     CDS             complement(28..219)
                     /locus_tag="QUE34_RS28310"
                     /old_locus_tag="EsCd1KSP079_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            284..607
                     /locus_tag="QUE34_RS28315"
                     /old_locus_tag="EsCd1KSP079_05616"
     CDS             284..607
                     /locus_tag="QUE34_RS28315"
                     /old_locus_tag="EsCd1KSP079_05616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001734677.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLATIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIERELRSLRQVVREHGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            816..>1247
                     /locus_tag="QUE34_RS28320"
                     /pseudo=""
     CDS             816..>1247
                     /locus_tag="QUE34_RS28320"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1285..1803
                     /locus_tag="QUE34_RS28325"
                     /old_locus_tag="EsCd1KSP079_05617"
     CDS             1285..1803
                     /locus_tag="QUE34_RS28325"
                     /old_locus_tag="EsCd1KSP079_05617"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067503.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVLFLFTVSG
                     GTLWYLGGRIQANLEEIRIQEETLQKLNAKTWGVEFVQDGRRKFLVLPYGKSATVIPYQ
                     EKDWVQLTE"
                     /name="MbeB family mobilization protein"
     gene            1810..2043
                     /locus_tag="QUE34_RS28330"
                     /old_locus_tag="EsCd1KSP079_05618"
     CDS             1810..2043
                     /locus_tag="QUE34_RS28330"
                     /old_locus_tag="EsCd1KSP079_05618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137441.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNEQVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2655..2861)
                     /locus_tag="QUE34_RS28335"
                     /old_locus_tag="EsCd1KSP079_05620"
     CDS             complement(2655..2861)
                     /locus_tag="QUE34_RS28335"
                     /old_locus_tag="EsCd1KSP079_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001096539.1"
                     /translation="MAKLPQEKPKEVIQAGFDDVKKAYKKGAKTVNDTYAEQAGRDLQS
                     GDGKLMATYRRNKKAIDKLDAEG"
                     /name="hypothetical protein"
     gene            complement(2854..3237)
                     /locus_tag="QUE34_RS28340"
                     /old_locus_tag="EsCd1KSP079_05621"
     CDS             complement(2854..3237)
                     /locus_tag="QUE34_RS28340"
                     /old_locus_tag="EsCd1KSP079_05621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000065853.1"
                     /translation="MSNIVHLSDKKQKNKPVDISFDDYTDNLSKGFVKNLSNKQKKELI
                     SKIKAILKSRNVAFRSTPPSDWKEKSRTFAIFFPEKKIVFTLGVVNKLKIEDGWINHKI
                     SDTEDAVLSVGKIINLIEDSQHG"
                     /name="hypothetical protein"
     gene            complement(3628..4689)
                     /locus_tag="QUE34_RS28345"
                     /old_locus_tag="EsCd1KSP079_05622"
     CDS             complement(3628..4689)
                     /locus_tag="QUE34_RS28345"
                     /old_locus_tag="EsCd1KSP079_05622"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034299.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0009036 - type II site-specific deoxyribonuclease
                     activity [Evidence IEA]"
                     /GO_process="GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HindVP family restriction endonuclease"
                     /protein_id="WP_000034299.1"
                     /translation="MSKIESLYGLKNTNRKESDLWGKNQFNSSFPTSLACYMRDNKMNP
                     IYLYLDKDLKVHAKEVSFDFVFNTTLKNSDLEFQFEKKYEPYQQYAYDDIKGIDLVIKG
                     NNSFLKALEIKLTVLPDSTTYKENESEWSSEIVIRPASTSYCALGIMHSCSDKFHEIRK
                     IFESTCESIQHWGNETEVLSKKENILNCLNTFQENFLDYQKPFLMQPLWKTKGKSPILD
                     QNAFDIFIWSDFALCRTFIDRSSYNNSSASRLMRSSARLARMVYEIASKNKTNINRIYT
                     EMNFGYQSDKEFSLNGKITKAYMDHPRRTKPILPPSVLKEIILNGGQNKLSPERRFDQS
                     VYYTASHLFDEIK"
                     /name="HindVP family restriction endonuclease"
     gene            4777..5934
                     /locus_tag="QUE34_RS28350"
                     /old_locus_tag="EsCd1KSP079_05623"
     CDS             4777..5934
                     /locus_tag="QUE34_RS28350"
                     /old_locus_tag="EsCd1KSP079_05623"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219937.1"
                     /GO_function="GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO:0003677 - DNA binding [Evidence IEA]; GO:1904047 -
                     S-adenosyl-L-methionine binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="WP_001219937.1"
                     /translation="MRIIDLFSGCGGLSLGFLKGGFDVVGAYDFWDPAIECYRDNFSHP
                     IKKLDLSNVDDVVRELKDIDFDMIIGGPPCQDFSHAGLRIEGARANLTRSFSEIIKRIK
                     PKWFVMENVDRALRSGAYLEARGIFKESGYGLTEIVLDASKCGVPQKRKRLFVIGKLDV
                     RDGFILNEVMCGISKDSMTVRNYLGDSLGIEYYYRHPRNYNRRAIFSIDEPAPTVRGVN
                     RPIPDGYLGHAGDPVSISENVRPLTTFERARLQTFPEDFKFKGAKTNLEQMIGNAVPVE
                     LAKYVAVTIMEYEKKQVKGIYDKEGFRAWLLNEKKLTKRTSSDIISRCCRGVSFFDSEG
                     VDFYNCEIDEIIMKLERLESFVRLGVSLKSQLRRAFKLYYEYCRR"
                     /name="DNA cytosine methyltransferase"
     Typing          6818..6899
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.3400000000000002e-35"
     Typing          complement(6249..6798)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.118"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          598..2147
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.794"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgtgaccgtc tccgggagct gtatgtgtca gaggttttca ccgtcatccc cgaagcgtgc
       61 gaggcagctg cggtaaagct catcggcgtg gtcgtgaagc gattcacaga tgtctgcctg
      121 ttcatccgcg tccagttcgt tgagcttttc cagcagcgtt aatgtctggc ttctgataaa
      181 gcgggccatg ttaagggcgg ttttttcctg tttagtcact gatgcctccg tgtaaggggg
      241 gatttctgtt catggggtaa tgataccgat gaaacgcgag aggatgctca cgatacgggt
      301 tactgatgat gaacatgcac ggttactgga acgctgtgag ggtaaacagc tggcggtatg
      361 gatgcggcgg gtctgtctgg gtgagccggt tgcccgttcc gggaagctgc cgacactggc
      421 accgccgtta ctgcgccagc tggctaccat cgggaataat ctgaatcaga cggcgaggaa
      481 ggtgaacagc gggcagtggt cttccggtga ccgggttcag gtggtggccg cactgatggc
      541 catcgagaga gagttgcgca gcttacggca ggtcgtcagg gaacatggag cgcgggatga
      601 tagttaagtt tcatgccagg ggaaaaggcg gtggcagtgg tccggttgat tacctgctgg
      661 gaagggagcg taaccgggaa ggggcaacgg tgcttcaggg taatccggaa gaagtccggg
      721 aactcatcga tgccacgcca tttgcgaaga aatacacgtc aggtgttctg tcgttcgcgg
      781 agaaggagct gccgccggga ggacgtgaaa aggtgatggc gagctttgag cgtgtactga
      841 tgcccggtct cgaaaaaaat cagtacagca tcctgtgggt ggagcaccag gacaagggac
      901 ggcttgagct gaattttgtc attccgaaca tggagctaca gagcggaaaa cgcctccagc
      961 cgtactacga ccgggcagac aggccgagaa ttgatgcctg gcagacgctg gttaatcacc
     1021 attacgggct gcatgacccc aacgccccgg agaaccgccg gatactgact ctccctgata
     1081 acctgccgga aacgaaacag gcgcttgcag agagcgtcac acgcgggata gacgctcttt
     1141 atcatgtcgg ggagataaaa ggccgtcagg atgtgattca ggcgctcaca gaggcagggc
     1201 ttgaagtggt cagggtgacg cgaagcagta tcagcattgc agacccgaac ggcgggaaga
     1261 atatcagact gaaaggagca ttctatgagc aatcttttac agacgggcgc ggagttcgag
     1321 aaaaagctga aagagagagc cgaatctaca gagacaatgc tgaacgacga gttcagcaag
     1381 ctcgaaaaat ctgtaagcaa ggctgtgaca tcaaacgaga cgaaaatcaa agacgctata
     1441 gccctgttca cagcttcgac agaggaatca ctgaaaaaac accgggaagg ggtgaaagag
     1501 gcgatgatgc agcacaggaa ggacgtgtta aagctggcag ggaatacggg catgatgtta
     1561 ctggggatgg tccttttcct gtttaccgtg agtggcggga cgctctggta tcttggcggg
     1621 cggatacagg cgaacctgga agaaatcagg atacaggaag aaacattgca gaaactgaac
     1681 gcgaagacat ggggcgtgga gtttgtgcag gacgggagaa ggaaatttct ggtccttccg
     1741 tacgggaaat cggcaacggt gattccctat caggagaagg actgggtaca actgacggag
     1801 tgacacagag tgacggagct ggaaagacat ttgctgaacg ccttagagca actgcaacag
     1861 gactatatgc agcggctgag cgaatgggag agcgccttcg tggaattgca gaaaatgttt
     1921 tcgcttacgc aacgggacaa cgcgatgctg aacgagcagg tcatgcagtt gagtcagcag
     1981 gtgcagcact tgagcgagcg gacagaacgc ttgagccagt tatacagcga gaacaggaga
     2041 taagggacga acggctgata caggagcgcg aacatgagtt atccctggag cgtgagcgcc
     2101 agctggaaat acaggaacgg acactggatg gcccttcgct gggatggtaa tcagagacag
     2161 gctaaaatgt tgtcagcgcg tttccggtgc attttttggg ataatggttg gttcgggggc
     2221 accgggaacg cgcttcagtc agaccttgct gaaaacacag gaacgcgtta aaacgcatac
     2281 gggcgcgatt ctggtgtgtc ttttgttgct gtgttttgcc tctattgcca gatcgcgctc
     2341 attcccctga acttctgttt tcccgctccg gtccccgtct tcttcgcttc cgaaagcatg
     2401 aaaaaaatcc gtttcagctg tctctggctg gcaattcctg cgtgattcac atggctgcat
     2461 agctatgcat gtggttaaaa tttaccgggt gcgatcgcgg cagtttttcg ggtggtttgt
     2521 tgccattttt acctgtctgc tgccgtgatc gcaccgaacg cgtttctgcg gtgcgggcaa
     2581 cggatgttat ggtaaataca atatgcagcc agtcttagag acgaaaagtc cgcccagaag
     2641 actgactttt cagatcaacc ttcagcatct aacttgtcta tcgctttttt attgcgccga
     2701 taggtagcca ttagtttacc atcgcctgat tgtaagtcac gacctgcttg ctcagcatag
     2761 gtatcgttta cagtcttagc gcctttttta taggcctttt taacatcatc aaatccggct
     2821 tggatgactt ctttcggctt ttcttgaggt aatttagcca tgctgtgaat cctctataag
     2881 gttgattatt tttccaacag ataagacggc atcttctgtg tcgctgattt tatgatttat
     2941 ccaaccgtcc tctatcttta atttgttcac tactcctaat gtaaaaacaa tctttttttc
     3001 aggaaagaaa attgcaaagg ttctactttt ctctttccaa tcagagggcg gcgtcgaacg
     3061 gaacgctaca ttcctcgatt taagtatcgc tttgatttta ctaattaatt cttttttttg
     3121 tttattgctg agatttttga cgaacccttt gctgagattg tctgtgtaat catcaaaaga
     3181 aatatcaact ggtttgtttt tttgtttttt atcagataga tgaacaatgt ttgacactgg
     3241 atatcctgct ttttgatgaa tgaatactca gaagagtagc ggaactgtct tgttagtcac
     3301 agaggtagag tatcaattct acgatagatg ggttcgaaga tccggctgag accgttcagt
     3361 acgcggtcga gatgttatgt tcaatgtaat gaccgtcgaa attgccttta cgggcatgaa
     3421 gcaaccagga gcaatcattg gcaaaggtaa ctagagttca atgcatggta atcatttttg
     3481 agcttgtgat gcatagagtt ggttgctgtt ccattgcgtc tcaaactccg tcaagatgct
     3541 cgccgtaaaa gcgagcatgt ctagtatgcc tgttccgcta ctgatttttg atgggcgaat
     3601 tcagggctgg cgagccacca agaaaaatta ctttatttca tcaaacaggt gagatgcagt
     3661 ataataaacg ctttgatcaa agcgtctctc tgggcttaat ttattttgcc cgccatttaa
     3721 aattatttct tttaatactg atggtgggag gatgggtttt gttcttcttg ggtgatccat
     3781 ataagctttt gttatttttc cgtttagcga gaactcttta tcactttgat atccaaagtt
     3841 catctcagta taaattctat tgatgtttgt cttgtttttg ctcgcaattt catacaccat
     3901 tctggcgagt ctggctgatg atctcattag tctgctggca gatgaattgt tatatgagga
     3961 tctatcaata aaggttcgac acaaagcaaa atcactccag atgaatatgt caaaggcatt
     4021 ttgatctaat attgggctct tgccttttgt tttccataat ggttgcatta aaaaaggttt
     4081 ctggtaatca agaaaatttt cttggaaagt gtttaggcaa tttaatatat tttctttctt
     4141 tgaaagaact tcagtttcat tcccccagtg ctgtatgctt tcgcatgtgg attcaaatat
     4201 ttttcttatt tcatggaatt tatcgctgca agagtgcatt atgccaaggg cacaataaga
     4261 agtgcttgca ggtcttatta caatctctga actccattcg ctctcgtttt ctttatatgt
     4321 cgtgctatct ggaagtacgg ttaattttat ttctagtgct tttaaaaaac tattgtttcc
     4381 ttttataact agatctattc cttttatgtc atcataagca tactgttggt atggttcata
     4441 ttttttctca aattgaaatt caagatcact attttttaat gttgtgttaa aaacgaagtc
     4501 aaaagatact tctttggcat gcacttttaa atccttatct aaataaagat aaatcggatt
     4561 cattttgttg tctctcatat aacatgctag agaggtggga aaagaactgt taaattggtt
     4621 ctttccccac agatcgctct ctttcctgtt ggtgtttttt aaaccataaa gagattctat
     4681 tttgctcact ttttgctcct gacgtcaatt tattgacgtc tatggtgtgt ttctatatca
     4741 tgatgtggag tgatatgata atgagttttt tatgttatga gaataattga tctttttagt
     4801 gggtgtgggg ggttgtccct aggctttttg aagggggggt ttgatgttgt aggtgcttat
     4861 gatttctggg atcctgctat tgaatgttac agagataatt ttagccatcc gataaaaaaa
     4921 ctagatttga gcaatgttga tgatgtggtg agggagctta aagatataga cttcgatatg
     4981 attattggag ggcctccttg ccaagacttc agtcatgcag gtttaagaat tgaaggtgct
     5041 agagctaact taactcgctc attttcagaa attataaaaa gaataaagcc aaagtggttt
     5101 gtgatggaaa atgtagatcg ggcattaaga agtggtgcct atttagaagc tcgaggaatc
     5161 tttaaagaat cagggtatgg gttaacagaa attgttttgg atgccagtaa gtgtggtgtt
     5221 ccgcaaaaaa gaaaaagatt atttgtaatt ggaaagcttg acgtgaggga tggttttatc
     5281 cttaacgaag tgatgtgcgg gatatcgaaa gatagtatga cagttagaaa ttatcttggt
     5341 gattcattgg ggattgagta ttattacaga catccgagaa attataacag aagggctatt
     5401 ttctctattg atgaacctgc gccaacagta agaggggtta atagaccgat acctgatgga
     5461 tatttagggc atgcaggcga tcctgtcagt atttctgaga atgtaaggcc attaacaacc
     5521 tttgaaagag caagattgca aacatttcct gaagacttta aatttaaagg tgcaaaaaca
     5581 aatttggagc aaatgatagg taatgctgtt ccggttgagt tagcaaaata tgttgcggtg
     5641 acaatcatgg agtatgaaaa aaaacaagta aaaggtattt atgataaaga aggctttagg
     5701 gcgtggttgc tgaatgaaaa gaaactaaca aaaagaacct ctagcgatat tatatctaga
     5761 tgttgcagag gtgtttcatt ttttgattca gagggggtgg atttttataa ctgtgaaata
     5821 gatgaaatta ttatgaaact ggaaaggctt gaatcgtttg tcaggcttgg ggttagcctg
     5881 aagtctcagt taagaagggc ttttaaatta tattacgaat attgtcgaag gtaataaata
     5941 cgaatttatg tctctcaggg gagggggagg ggataaaaag atcaaaggat cctcttgtga
     6001 tccttttttt ctgcgcgtaa tctgctgcct gtaaacaaaa aaaccaccgc taccaacggt
     6061 ggtttgtttg ccggaatcta gagctaccaa ctctttcttc cgaaggtaac tggcttggtg
     6121 gagcgcagat accaaatact gtccttctag tgtagccgca gttaggccac cacttcaaga
     6181 actcttaata tctcaatcca ccttgtccag ttaccagtgg ctgctgccag tggcgttaag
     6241 tcgtgtcttt ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     6301 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     6361 taccaacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     6421 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     6481 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttctg
     6541 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcggcgcggc cttttgttgt
     6601 cctttgcctt tttgctcaca tgttctttcc ggccttatcc cctgattctg tggataaccg
     6661 tattaccgcc tttgagtaag ccgactccgc tcgccgcagc cgaacgaccg agcgtagcga
     6721 gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg
     6781 cggtatttca caccgcatat ggtgctcttt tcttataatc cgcactgatg ccacatagtt
     6841 aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg
     6901 caaggtcaac ggcctgacgc gccctgacgg gcttgtctgc tcccggcatc cgctcacaga
     6961 caagc
//
