LOCUS       NZ_AP025744             7166 bp    DNA     circular CON 24-JUN-2023
DEFINITION  Escherichia sp. 10290 plasmid pla3, complete sequence.
ACCESSION   NZ_AP025744
VERSION     NZ_AP025744.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00467134
            Sequence Read Archive: DRR360889
            Assembly: GCF_030295155.1
KEYWORDS    RefSeq.
SOURCE      Escherichia sp. 10290
  ORGANISM  Escherichia sp. 10290
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Ogura,Y. and Okuno,M.
  TITLE     Escherichia cryptic clade I strains
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7166)
  AUTHORS   Ogura,Y. and Okuno,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAY-2022) Contact:Yoshitoshi Ogura Kurume University
            School of Medicine, Department of Infectious Medicine; Asahi-machi
            67, Kurume, Fukuoka 830-0011, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.4
            Genome Coverage                   :: 61x
            Sequencing Technology             :: MinION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/22/2023 12:30:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,882
            CDSs (total)                      :: 5,747
            Genes (coding)                    :: 5,497
            CDSs (with protein)               :: 5,497
            Genes (RNA)                       :: 135
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 100
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 87 of 250
            Pseudo Genes (incomplete)         :: 167 of 250
            Pseudo Genes (internal stop)      :: 44 of 250
            Pseudo Genes (multiple problems)  :: 43 of 250
            Pseudo Genes (short protein)      :: 3 of 250
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP025744.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7166
                     /organism="Escherichia sp. 10290"
                     /mol_type="genomic DNA"
                     /strain="10290"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:2935450"
                     /plasmid="pla3"
                     /geo_loc_name="Japan"
                     /collection_date="2002"
     gene            complement(join(6862..7166,1..19))
                     /locus_tag="QUE74_RS29310"
     CDS             complement(join(6862..7166,1..19))
                     /locus_tag="QUE74_RS29310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_000955995.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIERELRSLRQVVREHGAR
                     DDS"
                     /name="plasmid mobilization relaxosome protein MobC"
     gene            84..275
                     /locus_tag="QUE74_RS29315"
                     /old_locus_tag="EsCdI10290_05813"
     CDS             84..275
                     /locus_tag="QUE74_RS29315"
                     /old_locus_tag="EsCdI10290_05813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1321..2478)
                     /locus_tag="QUE74_RS29320"
                     /old_locus_tag="EsCdI10290_05814"
     CDS             complement(1321..2478)
                     /locus_tag="QUE74_RS29320"
                     /old_locus_tag="EsCdI10290_05814"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219937.1"
                     /GO_function="GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO:0003677 - DNA binding [Evidence IEA]; GO:1904047 -
                     S-adenosyl-L-methionine binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="WP_001219937.1"
                     /translation="MRIIDLFSGCGGLSLGFLKGGFDVVGAYDFWDPAIECYRDNFSHP
                     IKKLDLSNVDDVVRELKDIDFDMIIGGPPCQDFSHAGLRIEGARANLTRSFSEIIKRIK
                     PKWFVMENVDRALRSGAYLEARGIFKESGYGLTEIVLDASKCGVPQKRKRLFVIGKLDV
                     RDGFILNEVMCGISKDSMTVRNYLGDSLGIEYYYRHPRNYNRRAIFSIDEPAPTVRGVN
                     RPIPDGYLGHAGDPVSISENVRPLTTFERARLQTFPEDFKFKGAKTNLEQMIGNAVPVE
                     LAKYVAVTIMEYEKKQVKGIYDKEGFRAWLLNEKKLTKRTSSDIISRCCRGVSFFDSEG
                     VDFYNCEIDEIIMKLERLESFVRLGVSLKSQLRRAFKLYYEYCRR"
                     /name="DNA cytosine methyltransferase"
     gene            2566..3627
                     /locus_tag="QUE74_RS29325"
                     /old_locus_tag="EsCdI10290_05815"
     CDS             2566..3627
                     /locus_tag="QUE74_RS29325"
                     /old_locus_tag="EsCdI10290_05815"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034299.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0009036 - type II site-specific deoxyribonuclease
                     activity [Evidence IEA]"
                     /GO_process="GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HindVP family restriction endonuclease"
                     /protein_id="WP_000034299.1"
                     /translation="MSKIESLYGLKNTNRKESDLWGKNQFNSSFPTSLACYMRDNKMNP
                     IYLYLDKDLKVHAKEVSFDFVFNTTLKNSDLEFQFEKKYEPYQQYAYDDIKGIDLVIKG
                     NNSFLKALEIKLTVLPDSTTYKENESEWSSEIVIRPASTSYCALGIMHSCSDKFHEIRK
                     IFESTCESIQHWGNETEVLSKKENILNCLNTFQENFLDYQKPFLMQPLWKTKGKSPILD
                     QNAFDIFIWSDFALCRTFIDRSSYNNSSASRLMRSSARLARMVYEIASKNKTNINRIYT
                     EMNFGYQSDKEFSLNGKITKAYMDHPRRTKPILPPSVLKEIILNGGQNKLSPERRFDQS
                     VYYTASHLFDEIK"
                     /name="HindVP family restriction endonuclease"
     gene            4232..4615
                     /locus_tag="QUE74_RS29330"
                     /old_locus_tag="EsCdI10290_05816"
     CDS             4232..4615
                     /locus_tag="QUE74_RS29330"
                     /old_locus_tag="EsCdI10290_05816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_027663147.1"
                     /translation="MSNIVHLSDKKQKNKPVDISFDDYTDNLSKGFVKNLSNKQKKELI
                     SKIKAILKSRNVAFRSTPPSDWKEKSRTFAIFFPEKKVVFTLGVVNKLKIEDGWINHKI
                     SDTEDAVLSVGKIINLIEDSQHG"
                     /name="hypothetical protein"
     gene            4608..4814
                     /locus_tag="QUE74_RS29335"
                     /old_locus_tag="EsCdI10290_05817"
     CDS             4608..4814
                     /locus_tag="QUE74_RS29335"
                     /old_locus_tag="EsCdI10290_05817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_027663146.1"
                     /translation="MAKLPQEKPKEVIQAGFDDVKKAYKKGAKTVNDTYAEQAGRDLQS
                     GDGELMATYRRNKKAIDKLDAEG"
                     /name="hypothetical protein"
     gene            complement(5426..5659)
                     /locus_tag="QUE74_RS29340"
                     /old_locus_tag="EsCdI10290_05819"
     CDS             complement(5426..5659)
                     /locus_tag="QUE74_RS29340"
                     /old_locus_tag="EsCdI10290_05819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_001555310.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMKLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(5666..6184)
                     /locus_tag="QUE74_RS29345"
                     /old_locus_tag="EsCdI10290_05820"
     CDS             complement(5666..6184)
                     /locus_tag="QUE74_RS29345"
                     /old_locus_tag="EsCdI10290_05820"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_136766259.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSNE
                     TKIRDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTVSG
                     GTLWYLGGMIQANLEDIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPHQ
                     GKDWVQLME"
                     /name="MbeB family mobilization protein"
     gene            complement(<6222..6653)
                     /locus_tag="QUE74_RS29350"
                     /pseudo=""
     CDS             complement(<6222..6653)
                     /locus_tag="QUE74_RS29350"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          complement(354..438)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.837"
                     /coverage="100.0"
                     /evalue="1.41e-35"
     Typing          458..1007
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.937"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(5323..6872)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.412"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 taacccgtat cgtgagcatc ctctcgcgtt tcatcggtat cattacccca tgaacagaaa
       61 tccctcctta cacggaggca tcagtgacta aacaggaaaa aaccgccctt aacatggccc
      121 gctttataag aagccagaca ttaacgctgc tggaaaagct caacgaactg gacgcggatg
      181 aacaggcaga catctgtgaa tcgcttcacg accacgccga tgagctttac cgcagctgcc
      241 tcgcacgctt cggggatgac ggtgaaaacc tctgacacat acagctcccg gagacggtca
      301 cagcttgtct gtgagcggat gccgggagca gacaagcccg tcagggcgcg tcagggggtt
      361 tcggggcgaa gccctgaacc agtcacgtag cgatagcgga gtgtatactg gcttaactat
      421 gtggcatcag tgcagattat aagaaaagag caccatatgc ggtgtgaaat accgcacaga
      481 tgcgtaagga gaaaataccg catccggcgc tcttccgctt cctcgctcac tgactcgcta
      541 cgctcggtcg ttcggctgcg gcgagcggag tcggctcact caaaggcggt aatacggtta
      601 tccacagaat caggggataa gaccggaaag aacatgtgag caaaaaggca aaggacaaca
      661 aaaggccgcg ccgctggcgt ttttccatag gctccgcccc cctgacgagc atcacagaaa
      721 tcgacgctca agccagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
      781 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
      841 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgtt ggtatctcag
      901 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
      961 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacaaagc
     1021 gccactggca gcagccactg gtaactggac aaggtggatt gagatattaa gagttcttga
     1081 agtggtggcc taactgcggc tacactagaa ggacagtatt tggtatctgt gctccaccaa
     1141 gccagttacc ttcggaagaa agagttggta gctctagatt ccggcaaaca aaccaccgtt
     1201 ggtagcggtg gtttttttgt ttacaggcag cagattacgc gcagaaaaaa aggatcacaa
     1261 gaggatcctt tgatcttttt atcccctccc cctcccctga gagacataaa ttcgtattta
     1321 ttaccttcga caatattcgt aatataattt aaaagccctt cttaactgag acttcaggct
     1381 aaccccaagc ctgacaaacg attcaagcct ttccagtttc ataataattt catctatttc
     1441 acagttataa aaatccaccc cctctgaatc aaaaaatgaa acacctctgc aacatctaga
     1501 tataatatcg ctagaggttc tttttgttag tttcttttca ttcagcaacc acgccctaaa
     1561 gccttcttta tcataaatac cttttacttg ttttttttca tactccatga ttgtcaccgc
     1621 aacatatttt gctaactcaa ccggaacagc attacctatc atttgctcca aatttgtttt
     1681 tgcaccttta aatttaaagt cttcaggaaa tgtttgcaat cttgctcttt caaaggttgt
     1741 taatggcctt acattctcag aaatactgac aggatcgcct gcatgcccta aatatccatc
     1801 aggtatcggt ctattaaccc ctcttactgt tggcgcaggt tcatcaatag agaaaatagc
     1861 ccttctgtta taatttctcg gatgtctgta ataatactca atccccaatg aatcaccaag
     1921 ataatttcta actgtcatac tatctttcga tatcccgcac atcacttcgt taaggataaa
     1981 accatccctc acgtcaagct ttccaattac aaataatctt tttctttttt gcggaacacc
     2041 acacttactg gcatccaaaa caatttctgt taacccatac cctgattctt taaagattcc
     2101 tcgagcttct aaataggcac cacttcttaa tgcccgatct acattttcca tcacaaacca
     2161 ctttggcttt attcttttta taatttctga aaatgagcga gttaagttag ctctagcacc
     2221 ttcaattctt aaacctgcat gactgaagtc ttggcaagga ggccctccaa taatcatatc
     2281 gaagtctata tctttaagct ccctcaccac atcatcaaca ttgctcaaat ctagtttttt
     2341 tatcggatgg ctaaaattat ctctgtaaca ttcaatagca ggatcccaga aatcataagc
     2401 acctacaaca tcaaaccccc ccttcaaaaa gcctagggat aaccccccac acccactaaa
     2461 aagatcaatt attctcataa cataaaaaac tcattatcat atcactccac atcatgatat
     2521 agaaacacac catagacgtc aataaattga cgtcaggagc aaaaagtgag caaaatagaa
     2581 tctctttatg gtttaaaaaa caccaacagg aaagagagcg atctgtgggg aaagaaccaa
     2641 tttaacagtt cttttcccac ctctctagca tgttatatga gagacaacaa aatgaatccg
     2701 atttatcttt atttagataa ggatttaaaa gtgcatgcca aagaagtatc ttttgacttc
     2761 gtttttaaca caacattaaa aaatagtgat cttgaatttc aatttgagaa aaaatatgaa
     2821 ccataccaac agtatgctta tgatgacata aaaggaatag atctagttat aaaaggaaac
     2881 aatagttttt taaaagcact agaaataaaa ttaaccgtac ttccagatag cacgacatat
     2941 aaagaaaacg agagcgaatg gagttcagag attgtaataa gacctgcaag cacttcttat
     3001 tgtgcccttg gcataatgca ctcttgcagc gataaattcc atgaaataag aaaaatattt
     3061 gaatccacat gcgaaagcat acagcactgg gggaatgaaa ctgaagttct ttcaaagaaa
     3121 gaaaatatat taaattgcct aaacactttc caagaaaatt ttcttgatta ccagaaacct
     3181 tttttaatgc aaccattatg gaaaacaaaa ggcaagagcc caatattaga tcaaaatgcc
     3241 tttgacatat tcatctggag tgattttgct ttgtgtcgaa cctttattga tagatcctca
     3301 tataacaatt catctgccag cagactaatg agatcatcag ccagactcgc cagaatggtg
     3361 tatgaaattg cgagcaaaaa caagacaaac atcaatagaa tttatactga gatgaacttt
     3421 ggatatcaaa gtgataaaga gttctcgcta aacggaaaaa taacaaaagc ttatatggat
     3481 cacccaagaa gaacaaaacc catcctccca ccatcagtat taaaagaaat aattttaaat
     3541 ggcgggcaaa ataaattaag cccagagaga cgctttgatc aaagcgttta ttatactgca
     3601 tctcacctgt ttgatgaaat aaagtaattt ttcttggtgg ctcgccagcc ctgaattcgc
     3661 ccatcaaaaa tcagtagcgg aacaggcata ctagacatgc tcgcttttac ggcgagcatc
     3721 ttgacggagt ttgagacgca atggaacagc aaccaactct atgcatcaca agctcaaaaa
     3781 tgattaccat gcattgaact ctagttacct ttgccaatga ttgctcctgg ttgcttcatg
     3841 cccgtaaagg caatttcgac ggtcattaca ttgaacatgg ggtttgaggg ccaaccgtac
     3901 gaaaatgtac ggtaagagga aaattatcgt cttaaaaatc gattagtaga caatgccgtc
     3961 cgttaagtgc caattttcga ttaaaaagac accgttttga tggcgttttt ctatgtctat
     4021 tatgtcttga tatatcatac agttacttca ctaacgtacg ttttcgttcc attggacttc
     4081 agaccccaac ataacatctc gaccgcgtac tgaacggtct cagccggatc ttcgaaccca
     4141 tctatcgtag aattgatact ctacctctgt gactaacaag acagttccgc tactcttctg
     4201 agtattcatt catcaaaaag caggatatcc agtgtcaaac attgttcatc tatctgataa
     4261 aaaacaaaaa aacaaaccag ttgatatttc ttttgatgat tacacagaca atctcagcaa
     4321 agggttcgtc aaaaatctca gcaataaaca aaaaaaagaa ttaattagta aaatcaaagc
     4381 gatacttaaa tcgaggaatg tagcgttccg ttcgacgccg ccctctgatt ggaaagagaa
     4441 aagtagaacc tttgcaattt tctttcctga aaaaaaggtt gtttttacat taggagtagt
     4501 gaacaaatta aagatagagg acggttggat aaatcataaa atcagcgaca cagaagatgc
     4561 cgtcttatct gttggaaaaa taatcaacct tatagaggat tcacagcatg gctaaattac
     4621 ctcaagaaaa gccgaaagaa gtcatccaag ccggatttga tgatgttaaa aaggcctata
     4681 aaaaaggcgc taagactgta aacgatacct atgctgagca agcaggtcgt gacttacaat
     4741 caggcgatgg tgaactaatg gctacctatc ggcgcaataa aaaagcgata gacaagttag
     4801 atgctgaagg ttgatctgaa aagtcagtct tctgggcgga cttttcgtct ctaagactgg
     4861 ctgcatattg tatttaccat aacatccgtt gcacgcaccg cagaaacgcg ttcggtgcga
     4921 tcacggcagc agacaggtaa aaatggcaac aaaccacccg gaaaactgcc gcgatcgcac
     4981 ccggtaaatt ttaaccacat gcatagctat gcagccatgt gaatcacgca ggaattgcca
     5041 gccagagact gctgaaacgg atttttttca tgctttcgga agcgaagaag acggggaccg
     5101 gagcgggaaa acagaagttc aggggaatga gcgcgatctg gcaatagagg caaaacacag
     5161 caacaaaaga cacaccagaa tcgcgcccgt atgcgtttta acgcgttcct gtgttttcag
     5221 caaggtctga ctgaagcgcg ttcccggtgc ccccgaacca accattatcc caaaaaatgc
     5281 accggaaacg cgctgacaac attttagcct gtctctgatt accatcccag cgaagggcca
     5341 tccagtgtcc gttcctgtat ttccagctgg cgctcacgct ccagggataa ctcatgttcg
     5401 cgttcctgta tcagccgttc gtcccttatc tcctgttctc gctgtataac tggctcaagc
     5461 gttctgtccg ctcgctcaag tgctgcacct gctgactcaa cttcatgacc cgctcgttca
     5521 gcatcgcgtt gtcccgttgc gtaagcgaaa acattttctg caattccacg aaggcgctct
     5581 cccattcgtt cagccgctgc atatagtcct gttgcagttg ctctaatgcg ttcagcaaat
     5641 gtctttccag ctccgtcact ctgtgtcact ccatcagttg tacccagtcc ttcccctgat
     5701 ggggaatcac ctctgctgat ttcccgtacg gaaggaccag aaatttcctg ttcccgtcct
     5761 gtacgaactc cacgccccat gtcttcgcgt tcagtttctg caatgtctct tcctgcttcc
     5821 tgatatcttc caggttcgcc tgtatcatcc ctccaagata ccagagcgtc ccgccactca
     5881 cggtaaacag gaaaatgacc atccccagta acatcatgcc cgtattccct gccagcttta
     5941 acacgtcctt cctgtgctgc atcatcgcct ctttcacccc ttcccggtgt tttttcagtg
     6001 attcctctgt cgaagctgtg aacagggcta tagcgtctct gattttcgtc tcgtttgatg
     6061 tcacagcctt gcttacagat ttttcgagct tgctgaactc gtcgttcagc attgtctctg
     6121 tagattcggc tctctctttc agctttttct cgaactccgc gcccgtctgt aaaagattgc
     6181 tcataaaatg ctcctttcag cctgatattc ttcccaccgt tcgggtctgc aatgctgata
     6241 ctgcttcgcg tcaccctgac cacttcaagc cctgcctctg tgagcgcctg aatcacatcc
     6301 tgacggcctt ttatctcccc gacatgataa agagcgtcta tcccgcgtgt gacgctctct
     6361 gcaagcgcct gtttcgtttc cggcaggtta tcaggaaggg tcagcgtccg gcggttctcc
     6421 ggggcgttcg ggtcatgcag cccgtaatgg tgattcacca gcgtctgcca ggcatcaatt
     6481 ctcggcctgt ctgcccggtc gtagtacggc tgtaggcgtt ttccggtctc cagctccatg
     6541 ttcggaatga caaaattcag ctcaagccgt cccttgtcct ggtgctccac ccacaggatg
     6601 ctgtactgat ttttttcgag accgggcatc agtacacgct caaagctcgc catcactttt
     6661 tcacgtcctc ccggcggcag ctccttctcc gcgaacgaca gaacaccgga cgtgtatttc
     6721 ttcgcaaatg gcgtggcatc gatgagttcc cggacttctt ccggattacc ctgaagcacc
     6781 gttgcccctt cccggttacg ctcccttccc agcaggtaat caaccggacc actgccaccg
     6841 ccttttcccc tggcatgaaa cttaactatc atcccgcgct ccatgttccc tgacgacctg
     6901 ccgtaagctg cgcaactctc tctcgatggc catcagtgcg gccaccacct gaacccggtc
     6961 accggaagac cactgcccgc tgttcacctt cctcgccgtc tgattcaggt tattcccgat
     7021 ggcagccagc tggcgcagta acggcggtgc cagtgtcggc agcttcccgg aacgggcaac
     7081 cggctcaccc agacagaccc gccgcatcca taccgccagc tgtttaccct cacagcgttc
     7141 cagtaaccgt gcatgttcat catcag
//
