LOCUS       NZ_AP025666             2345 bp    DNA     circular CON 07-JUN-2024
DEFINITION  Escherichia coli strain TUM20902 plasmid pMTY20902_untypable2,
            complete sequence.
ACCESSION   NZ_AP025666
VERSION     NZ_AP025666.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00441898
            Sequence Read Archive: DRR345529, DRR345533
            Assembly: GCF_023170005.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Tsutsumi,Y., Hamada,M., Aoki,K., Komori,K., Miura,S., Ishii,Y. and
            Tateda,K.
  TITLE     Whole-genome sequence of mutant strains analysis by carbapenem
            exposure
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Komori,K. and Aoki,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-MAR-2022) Contact:Kohji Komori Toho University, Toho
            University School of Medicine, Department of Microbiology and
            Infectious Diseases; 5-21-16, Omori-nishi, Ota-ku, Tokyo 143-8540,
            Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8-beta
            Genome Coverage                   :: 356x
            Sequencing Technology             :: Illumina MiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023170005.1-RS_2024_06_07
            Annotation Date                   :: 06/07/2024 02:32:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,004
            CDSs (total)                      :: 4,886
            Genes (coding)                    :: 4,724
            CDSs (with protein)               :: 4,724
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 59 of 162
            Pseudo Genes (incomplete)         :: 114 of 162
            Pseudo Genes (internal stop)      :: 31 of 162
            Pseudo Genes (multiple problems)  :: 38 of 162
            Pseudo Genes (short protein)      :: 1 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP025666.1.
            Annotated by DFAST v.1.2.14 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="TUM20902"
                     /db_xref="taxon:562"
                     /plasmid="pMTY20902_untypable2"
                     /geo_loc_name="Japan"
                     /collection_date="2021"
     gene            complement(919..2091)
                     /locus_tag="M2084_RS24920"
                     /old_locus_tag="TUM20902_47610"
     CDS             complement(919..2091)
                     /locus_tag="M2084_RS24920"
                     /old_locus_tag="TUM20902_47610"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_097464546.1"
                     /translation="MDLTIEQRRIANAKIMIAKELATNSGTFIEIQIDRMIKEIISKVK
                     ISASDASKEQYRKLYKSCTSNSGGDLKSCLMNCKTRASFDKTRTSFRFCIMEEIQQLRR
                     ESEKAMKSKDYELGKKKIQEAFKLAVKFEDEFLSENRIIFNDVKNQKGFKRVSFSKRKG
                     LKKAASEEDIIKSFGTNDKVHERYSLAFSVIATFGIRPAELQKGVKITFQDGMIYAVAK
                     GAKTGENKGQKVRAMGSALRDNNICDQVLMNAIYNNGGEVVITQNKKDYDGLRKYLNRH
                     HKGTSLYTYRHKVASNLKATGASKRQIAEFLGHRTTESQQHYGYSRSGSGGHSMTAKGS
                     NEVRVKPDIKDYLKPATKPAAVPLGSKLKQAKTSISTTSKAPKFKPPGIK"
                     /name="hypothetical protein"
ORIGIN
        1 ttattctgtc tatctatcca taaacagaga gagtgcggag caaaaagtgg catcgccaaa
       61 aaaaatgttt ttccggtttt tagtccggtg cgtaagtgct ctatattcgt ttagtggata
      121 ttacgcttcc tcgacaaaat aaaatatttt gtcttgcggc gagcgttgct tactttttct
      181 tgtcggcggt ttttctttta ccttatagct gtactaactc tgatcgagcc ttcggcctgt
      241 gctacgccat aagaaccaca aaggaagctc cgccccccta aagggtcgct tcacccaaag
      301 tttcgcctcc caaaatcatt acgatttcag gcagacgatc ccttggtaaa gcatagcttt
      361 ttgagcttca cccaaagagc agcgacttag agctgtacaa cctttcgatc atactgacac
      421 cctgcagcac ccccgctgtc tccacatatc gtaacttacg gcatctaacc ccatttggct
      481 gggctttctc atagctggcc ttgatgctca tcatacgact tagcagatcg tttccggttc
      541 ggtcttttgc tgcactcccc gtttagaccc acatacactg tttggcgaac aatcacatgc
      601 cagtcacttg gaaaggcggc tccacaccaa acaccggaca caatcggttt aacaaactat
      661 gtatgcttaa ggatatgaaa atgcaggtga ttttatgaaa cttgtaaaga agtgggattt
      721 gacttttacg gaaatgtaag ttaacttttg ccttacatca cgtaatcatg tgctcgaatc
      781 acttgattac aagactcaaa aagtcgccaa actttttcag tccctcaaaa ccagcgcaag
      841 ctggttttgt tgtatctgga tcctgttata actgatttgc gttatacgtc aatagtctat
      901 caaaaatatt gaagcatctt atttgatacc cggaggctta aacttcggcg ctttcgacgt
      961 ggtggaaatc gaggttttag cttgcttgag cttgctacct aaaggcactg cagcaggctt
     1021 tgtggctggt tttaagtagt cttttatatc tggtttaacc cttacttcat tagacccctt
     1081 agccgtcata gaatggccgc cagatccaga tctggagtat ccatagtgct gttgtgactc
     1141 ggtagttcta tgccccaaaa actctgcgat ctgacgctta gaagcacctg tggccttgag
     1201 gttgctcgca actttgtgac gataggtata aagcgaggtt cctttatgat gtctatttaa
     1261 atactttcgc aaaccatcat aatctttttt attttgcgtg ataacaacct cgccgccatt
     1321 attataaata gcattcatca aaacttgatc acaaatatta ttatctctta aagccgaacc
     1381 catagccctt actttctgac ctttattttc tcctgtttta gctcctttcg ctacggcata
     1441 aatcatacca tcttggaatg tgatttttac acctttctgt agttctgctg gtctgattcc
     1501 aaacgttgct atgacagaga acgccagaga gtagcgttca tgcactttat cattagtgcc
     1561 aaatgatttt attatatctt cttccgaagc tgctttttta agaccttttc ttttactgaa
     1621 cgaaactctt ttaaaacctt tttgattttt gacatcatta aaaataattc tattttcaga
     1681 taaaaattca tcttcaaatt taactgctag tttaaatgct tcttgaattt ttttctttcc
     1741 gagttcgtaa tctttagact tcatcgcctt ttcagattca cgccttaact gctgtatttc
     1801 ttccataata cagaacctga atgacgttct tgttttatca aagcttgcac gagttttaca
     1861 gttcatcaag cagcttttca gatctccacc gctattggat gtgcatgatt tatataactt
     1921 tctgtactgc tccttggatg catcgctagc cgagatctta actttgctta ttatttcttt
     1981 tatcattctg tcaatttgaa tttcgataaa agtaccgcta ttggttgcta actcttttgc
     2041 gatcattatt tttgcattag cgatccttct ttgttctatt gttaaatcca tattatcccc
     2101 cctgctgtat ttttattcct ctatcgtctt tatatgccca tctcccaatg cgaaaggtca
     2161 gaaagctgac gcaatcttcc caatcgcaaa ggtcgaaggg caaggcgttt ttgctaccgc
     2221 aaacttccat tttcaaaagc gaaaatgtaa gacacgccca cattattggg acataaaaac
     2281 actgtcccaa taatgttaaa agcataagtc ccaataatgt tatatatata ttttaacttt
     2341 tattt
//
