LOCUS       NZ_AP025661             2345 bp    DNA     circular CON 31-MAY-2024
DEFINITION  Escherichia coli strain TUM13867 plasmid pMTY13867_untypable2,
            complete sequence.
ACCESSION   NZ_AP025661
VERSION     NZ_AP025661.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00441897
            Sequence Read Archive: DRR345528, DRR345532
            Assembly: GCF_022846575.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Tsutsumi,Y., Hamada,M., Aoki,K., Komori,K., Miura,S., Ishii,Y. and
            Tateda,K.
  TITLE     Whole-genome sequence of mutant strains analysis by carbapenem
            exposure
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Komori,K. and Aoki,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-MAR-2022) Contact:Kohji Komori Toho University, Toho
            University School of Medicine, Department of Microbiology and
            Infectious Diseases; 5-21-16, Omori-nishi, Ota-ku, Tokyo 143-8540,
            Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8-beta
            Genome Coverage                   :: 197x
            Sequencing Technology             :: Illumina MiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022846575.1-RS_2024_05_31
            Annotation Date                   :: 05/31/2024 04:41:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,030
            CDSs (total)                      :: 4,912
            Genes (coding)                    :: 4,746
            CDSs (with protein)               :: 4,746
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 59 of 166
            Pseudo Genes (incomplete)         :: 119 of 166
            Pseudo Genes (internal stop)      :: 30 of 166
            Pseudo Genes (multiple problems)  :: 38 of 166
            Pseudo Genes (short protein)      :: 1 of 166
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP025661.1.
            Annotated by DFAST v.1.2.14 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="TUM13867"
                     /db_xref="taxon:562"
                     /plasmid="pMTY13867_untypable2"
                     /geo_loc_name="Japan"
                     /collection_date="2013"
     gene            671..1843
                     /locus_tag="MWM17_RS25070"
                     /old_locus_tag="TUM13867_47980"
     CDS             671..1843
                     /locus_tag="MWM17_RS25070"
                     /old_locus_tag="TUM13867_47980"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_097464546.1"
                     /translation="MDLTIEQRRIANAKIMIAKELATNSGTFIEIQIDRMIKEIISKVK
                     ISASDASKEQYRKLYKSCTSNSGGDLKSCLMNCKTRASFDKTRTSFRFCIMEEIQQLRR
                     ESEKAMKSKDYELGKKKIQEAFKLAVKFEDEFLSENRIIFNDVKNQKGFKRVSFSKRKG
                     LKKAASEEDIIKSFGTNDKVHERYSLAFSVIATFGIRPAELQKGVKITFQDGMIYAVAK
                     GAKTGENKGQKVRAMGSALRDNNICDQVLMNAIYNNGGEVVITQNKKDYDGLRKYLNRH
                     HKGTSLYTYRHKVASNLKATGASKRQIAEFLGHRTTESQQHYGYSRSGSGGHSMTAKGS
                     NEVRVKPDIKDYLKPATKPAAVPLGSKLKQAKTSISTTSKAPKFKPPGIK"
                     /name="hypothetical protein"
ORIGIN
        1 cagtatgatc gaaaggttgt acagctctaa gtcgctgctc tttgggtgaa gctcaaaaag
       61 ctatgcttta ccaagggatc gtctgcctga aatcgtaatg attttgggag gcgaaacttt
      121 gggtgaagcg accctttagg ggggcggagc ttcctttgtg gttcttatgg cgtagcacag
      181 gccgaaggct cgatcagagt tagtacagct ataaggtaaa agaaaaaccg ccgacaagaa
      241 aaagtaagca acgctcgccg caagacaaaa tattttattt tgtcgaggaa gcgtaatatc
      301 cactaaacga atatagagca cttacgcacc ggactaaaaa ccggaaaaac attttttttg
      361 gcgatgccac tttttgctcc gcactctctc tgtttatgga tagatagaca gaataaaaat
      421 aaaagttaaa atatatatat aacattattg ggacttatgc ttttaacatt attgggacag
      481 tgtttttatg tcccaataat gtgggcgtgt cttacatttt cgcttttgaa aatggaagtt
      541 tgcggtagca aaaacgcctt gcccttcgac ctttgcgatt gggaagattg cgtcagcttt
      601 ctgacctttc gcattgggag atgggcatat aaagacgata gaggaataaa aatacagcag
      661 gggggataat atggatttaa caatagaaca aagaaggatc gctaatgcaa aaataatgat
      721 cgcaaaagag ttagcaacca atagcggtac ttttatcgaa attcaaattg acagaatgat
      781 aaaagaaata ataagcaaag ttaagatctc ggctagcgat gcatccaagg agcagtacag
      841 aaagttatat aaatcatgca catccaatag cggtggagat ctgaaaagct gcttgatgaa
      901 ctgtaaaact cgtgcaagct ttgataaaac aagaacgtca ttcaggttct gtattatgga
      961 agaaatacag cagttaaggc gtgaatctga aaaggcgatg aagtctaaag attacgaact
     1021 cggaaagaaa aaaattcaag aagcatttaa actagcagtt aaatttgaag atgaattttt
     1081 atctgaaaat agaattattt ttaatgatgt caaaaatcaa aaaggtttta aaagagtttc
     1141 gttcagtaaa agaaaaggtc ttaaaaaagc agcttcggaa gaagatataa taaaatcatt
     1201 tggcactaat gataaagtgc atgaacgcta ctctctggcg ttctctgtca tagcaacgtt
     1261 tggaatcaga ccagcagaac tacagaaagg tgtaaaaatc acattccaag atggtatgat
     1321 ttatgccgta gcgaaaggag ctaaaacagg agaaaataaa ggtcagaaag taagggctat
     1381 gggttcggct ttaagagata ataatatttg tgatcaagtt ttgatgaatg ctatttataa
     1441 taatggcggc gaggttgtta tcacgcaaaa taaaaaagat tatgatggtt tgcgaaagta
     1501 tttaaataga catcataaag gaacctcgct ttatacctat cgtcacaaag ttgcgagcaa
     1561 cctcaaggcc acaggtgctt ctaagcgtca gatcgcagag tttttggggc atagaactac
     1621 cgagtcacaa cagcactatg gatactccag atctggatct ggcggccatt ctatgacggc
     1681 taaggggtct aatgaagtaa gggttaaacc agatataaaa gactacttaa aaccagccac
     1741 aaagcctgct gcagtgcctt taggtagcaa gctcaagcaa gctaaaacct cgatttccac
     1801 cacgtcgaaa gcgccgaagt ttaagcctcc gggtatcaaa taagatgctt caatattttt
     1861 gatagactat tgacgtataa cgcaaatcag ttataacagg atccagatac aacaaaacca
     1921 gcttgcgctg gttttgaggg actgaaaaag tttggcgact ttttgagtct tgtaatcaag
     1981 tgattcgagc acatgattac gtgatgtaag gcaaaagtta acttacattt ccgtaaaagt
     2041 caaatcccac ttctttacaa gtttcataaa atcacctgca ttttcatatc cttaagcata
     2101 catagtttgt taaaccgatt gtgtccggtg tttggtgtgg agccgccttt ccaagtgact
     2161 ggcatgtgat tgttcgccaa acagtgtatg tgggtctaaa cggggagtgc agcaaaagac
     2221 cgaaccggaa acgatctgct aagtcgtatg atgagcatca aggccagcta tgagaaagcc
     2281 cagccaaatg gggttagatg ccgtaagtta cgatatgtgg agacagcggg ggtgctgcag
     2341 ggtgt
//
