LOCUS       NZ_AP025533            10786 bp    DNA     circular CON 12-MAY-2024
DEFINITION  Acinetobacter baumannii strain OCU-Ac19 plasmid pOCUAc19-2, complete
            sequence.
ACCESSION   NZ_AP025533
VERSION     NZ_AP025533.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00258369
            Sequence Read Archive: DRR255177, DRR255178
            Assembly: GCF_027943685.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1
  AUTHORS   Fujimoto,R., Tsubouchi,T., Suzuki,M. and Kaneko,Y.
  TITLE     Complete genome sequence of Acinetobacter baumannii, clinical
            isolated
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10786)
  AUTHORS   Tsubouchi,T., Fujimoto,R., Suzuki,M. and Kaneko,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JAN-2022) Contact:Taishi Tsubouchi Osaka City
            University, Department of Bacteriology, Graduate School of Medicine;
            1-4-3, Asahimachi, Abeno, Osaka, Osaka 545-8585, Japan URL
            :http://www.med.osaka-cu.ac.jp/bacteriology/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: unicycler v. 0.4.8, Pilon v. 1.24
            Genome Coverage                   :: 1075x
            Sequencing Technology             :: Illumina NovaSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_027943685.1-RS_2024_05_12
            Annotation Date                   :: 05/12/2024 04:56:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,868
            CDSs (total)                      :: 3,777
            Genes (coding)                    :: 3,673
            CDSs (with protein)               :: 3,673
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 34 of 104
            Pseudo Genes (incomplete)         :: 64 of 104
            Pseudo Genes (internal stop)      :: 13 of 104
            Pseudo Genes (multiple problems)  :: 7 of 104
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP025533.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10786
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="OCU-Ac19"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="pOCUAc19-2"
                     /geo_loc_name="Japan:Osaka"
                     /lat_lon="34.6467 N 135.5088 E"
                     /collection_date="2016-08-20"
     gene            complement(150..767)
                     /locus_tag="QMN04_RS19265"
                     /old_locus_tag="OCUAc19_37680"
     CDS             complement(150..767)
                     /locus_tag="QMN04_RS19265"
                     /old_locus_tag="OCUAc19_37680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_025469582.1"
                     /translation="MKSLSVIELSNLYGITRQAIYKQVNKGNLSKNSDGKIDLSEAIRV
                     FGEPSRNVNSSQTTETRKLSEVHLLEQQVYMLQKQLEQAHEREQFQREELKAKNDQLHV
                     KDEQIEAIQRLLEAPKAYTTSSIDPKLDIATDARSKSESNYDVLTTQPRETPVEVEIQT
                     QPKDDGLTTPEQTENKRIPVPEHVEQEPEKRGFWSRFFRPYD"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(844..1743)
                     /gene="repM"
                     /locus_tag="QMN04_RS19270"
                     /old_locus_tag="OCUAc19_37690"
     CDS             complement(844..1743)
                     /gene="repM"
                     /locus_tag="QMN04_RS19270"
                     /old_locus_tag="OCUAc19_37690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016650500.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_004884485.1"
                     /translation="MMSTSKKELVVKSNQVIEASYQLSSTEQRIVLAAISKISRAEDIT
                     DDEIYRVTIDDLKKLGVHEKTAYRDLKDGVNRLYDRSINLAIDDESIKMRWIQSIRFLE
                     SKSVVGIRFSKEILPFISNLTREFTKYSLSDIAGMSSAYAIRIYELLSQYRSIGKREIS
                     IESLRSMLELGKRYPLSADLKRWVIDTAVDQINEHSPLNVSYEQIKTGRKVTHIHFTFK
                     EKSKSIEHKSEQNNFYKLTDSQINMFGNQLSRLHEVSHLAQQGESYDDLAIKIKDMLRD
                     PIKQKQLIPHLKNLGFKA"
                     /name="repM"
     gene            complement(2287..3456)
                     /locus_tag="QMN04_RS19275"
                     /old_locus_tag="OCUAc19_37700"
     CDS             complement(2287..3456)
                     /locus_tag="QMN04_RS19275"
                     /old_locus_tag="OCUAc19_37700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002011489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_004884505.1"
                     /translation="MATYHFSIKSKNKGYATQHYLYIARLMQYEGIRKKSEEVLEHVEP
                     GRCMPAFVKDPIEFWQAADSHERANAKAYMEYEIALPNEFTPEQRKTLIETFLDKHIVP
                     QQYPHSYAIHNVKSRISGEDQPHCHLMFSLKADDGIERSAEQYFKRYNPQDPAKGGAKK
                     IQLQDGYENYSTFLIYIRKQWENHLNDALAQHCPTVTYTLDGQDITIKNQVSADSYEKY
                     NEIHGTLYLPEPKLGVGQQNATAEYLAQIQKIREHNQQERKLEERQKQLDQQHEYFYSA
                     ENHPFSSLEVMYYLNYLVKNQKNKEKLEKDFSEIIKTQEPILHQEGWNLQNMNRKLRDS
                     EHNSTPCFEFEVHSQPKYPLPMQNKPPQSVQPEQPKPKSDDYDYGGPGF"
                     /name="MobA/MobL family protein"
     gene            3756..4532
                     /locus_tag="QMN04_RS19280"
                     /old_locus_tag="OCUAc19_37710"
     CDS             3756..4532
                     /locus_tag="QMN04_RS19280"
                     /old_locus_tag="OCUAc19_37710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004728463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004884503.1"
                     /translation="MEMEKAKKRGRPAQLLQRAELHAFVDFLLENNRRTELQNQVIDAL
                     EAEDFNFEMLSEAQKILVKETLKPYREHLKLQLLFDELSKNPRRTEYEAKFIELFHAYQ
                     DNQLGIAELNILKTMCTRYLNFKAQKLQYADLELYLSQIQKKDAGKKRKAENNRKYQLG
                     GAVIAAYKELGLNIDTLTPDQVRKAIVGNKRIINKIEKSIIFEQIDMDLDTLYFRWPIF
                     LKVIDKLAMIEYEGEVVPEYIKEIYLALSECKAGKK"
                     /name="hypothetical protein"
     gene            complement(4773..5018)
                     /locus_tag="QMN04_RS19285"
                     /old_locus_tag="OCUAc19_37720"
     CDS             complement(4773..5018)
                     /locus_tag="QMN04_RS19285"
                     /old_locus_tag="OCUAc19_37720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004728465.1"
                     /translation="MYSYPNSNAEKKIALMIINDFFIQKAHELWIFLQLDPSFNDYEAT
                     VIWTRRYLEEHPEGEYSDIQKAFLSCFPEHFFNFDY"
                     /name="hypothetical protein"
     gene            5170..5394
                     /locus_tag="QMN04_RS19290"
                     /old_locus_tag="OCUAc19_37730"
     CDS             5170..5394
                     /locus_tag="QMN04_RS19290"
                     /old_locus_tag="OCUAc19_37730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004676219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004884500.1"
                     /translation="MPYTEFQRLIGKAGLSIKEFAALLDMKPNSITNYSKQGVVPTHIA
                     VIIALISTMKDEGLDFYPIFEKIKSYSKE"
                     /name="hypothetical protein"
     gene            5682..5966
                     /locus_tag="QMN04_RS19295"
                     /old_locus_tag="OCUAc19_37740"
     CDS             5682..5966
                     /locus_tag="QMN04_RS19295"
                     /old_locus_tag="OCUAc19_37740"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098722690.1"
                     /translation="MDYKLIILSISIIFGFFSAGAWFYSSQVKVTREKAVKIAKKKAQK
                     KNEQPNLSGVSFDGWDVRETLKAQSKWQLHRIRDGLTTAYAYLTSSSKK"
                     /name="hypothetical protein"
     gene            complement(6009..6407)
                     /locus_tag="QMN04_RS19300"
                     /old_locus_tag="OCUAc19_37750"
     CDS             complement(6009..6407)
                     /locus_tag="QMN04_RS19300"
                     /old_locus_tag="OCUAc19_37750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="WP_098722691.1"
                     /translation="MKKIFSLIAIPVLTSISFLAVASDRPQEDARKMDESTVVRKAVNY
                     NCGHGQRLKVTYGFNKQGLPTYAQAYLSGKTRFMPINLNLSDMVGTTFGDDNNFSLHTA
                     GITSKKYRADIQVQSPSSEIIYKSCSAR"
                     /name="adhesin"
     gene            complement(6553..6792)
                     /locus_tag="QMN04_RS19305"
                     /old_locus_tag="OCUAc19_37760"
     CDS             complement(6553..6792)
                     /locus_tag="QMN04_RS19305"
                     /old_locus_tag="OCUAc19_37760"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098722692.1"
                     /translation="MQNDFMNAFNSLATDLEQQLNELQATTTKQSEQIERLTAVNRNLI
                     EQNERLNNENKQLANINAQQNDLLTKLLGKSKRN"
                     /name="hypothetical protein"
     gene            complement(6799..7044)
                     /locus_tag="QMN04_RS19310"
                     /old_locus_tag="OCUAc19_37770"
     CDS             complement(6799..7044)
                     /locus_tag="QMN04_RS19310"
                     /old_locus_tag="OCUAc19_37770"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098722693.1"
                     /translation="MTKSHALGIVSLYLAKTIRNYHEEIAEIQQNIEMTSLQAQAFAKV
                     TITQNKDKSIWITAKNPKKSESGKLESNGKPTLKLE"
                     /name="hypothetical protein"
     gene            7179..7781
                     /locus_tag="QMN04_RS19315"
                     /old_locus_tag="OCUAc19_37780"
     CDS             7179..7781
                     /locus_tag="QMN04_RS19315"
                     /old_locus_tag="OCUAc19_37780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004733280.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_126064970.1"
                     /translation="MNTRIYLRASTKDQDAERALQILQDLNQNLNLGETIVYVENYSGT
                     KLDRPELNKLLSQANQGDTLLVESIDRLSRLTQQDFQELKRRIQEKGLRLVVADLPTTY
                     QMIQTSDSITHSILELINNMLIDLLATMARLDNEKRIERIKQGLERSGYKPTGKKANEA
                     KHKRIKELLASGNMTKEEIAKAVNCGVATVYRVAKVI"
                     /name="recombinase family protein"
     gene            7807..8001
                     /locus_tag="QMN04_RS19320"
                     /old_locus_tag="OCUAc19_37790"
     CDS             7807..8001
                     /locus_tag="QMN04_RS19320"
                     /old_locus_tag="OCUAc19_37790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004733282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_159124526.1"
                     /translation="MSYLFTKEQGILKDQKTELMMKKHLIFLVFIVMVFILALVVKINN
                     DHKKAQALNKLELVEKKAN"
                     /name="hypothetical protein"
     gene            8503..9621
                     /locus_tag="QMN04_RS19325"
                     /old_locus_tag="OCUAc19_37800"
     CDS             8503..9621
                     /locus_tag="QMN04_RS19325"
                     /old_locus_tag="OCUAc19_37800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004755996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="WP_126064968.1"
                     /translation="MQKWIWQAENWTNFTWQDSTILPKTRQIHQKIGILLGQSQHDLAK
                     EQFTLDTLLANLVASSAIENETLNVFSLRSSLAQRLGVTLEQPYPSSDRSEGLANIMLD
                     ALNDVKQPLTVERLMQWHRWLFNDPDWTMQRLRIGQLRGSEPMQVVSGRLDYPKVHFEA
                     PPREGLEERLHTFIAWFNQSLNDSLLDPLLRAGITHLWFVTLHPFDDGNGRITRTLTDL
                     ALAQMDEQSIRLYAMSTAILNKRKSYYEILEQTQKNDSDITDWLIWFLDTLNESLEKTL
                     AQISRTLFKSQFWHKYSNLALGEEQRKVLNRLLDGGENGFEHGISASQYQKVAKVSKAT
                     ATRHLSDLLEKECIVKLEGGGRNTRYQINTQL"
                     /name="Fic family protein"
     gene            10222..10740
                     /locus_tag="QMN04_RS19330"
                     /old_locus_tag="OCUAc19_37810"
     CDS             10222..10740
                     /locus_tag="QMN04_RS19330"
                     /old_locus_tag="OCUAc19_37810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447193.1"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="WP_000447193.1"
                     /translation="METFAEPVFSNDLLAKAENGDTSAQLELAEIYLYGHGVDSDENQA
                     EVWALKSAENGNVAAMFWLADGYVTYARLMEDDDKNDSLEHFQKAFKWFQKASENGHSE
                     SMVELADLYTRADSGIEVNINKAFELREKAAKLGNKKAMRSLSVMYRDGIGIPKNTDLA
                     QSWWDKSEN"
                     /name="tetratricopeptide repeat protein"
     Typing          complement(845..1740)
                     /name="rep_cluster_1357"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.872"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2342..3456)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.398"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gttataatta tctatagaaa tcaatgttgt agtttctagt ttatagccca ccttccccaa
       61 ggtgggtttt ttttatgact tttcacgttc cacgcctatt ctttgatcaa tgtttcacga
      121 ctttattcat attagttaaa taatcgtgat taatcatagg gtctaaaaaa acggctccaa
      181 aagccccttt tctccggttc ctgttcaaca tgctccggca cggggatccg cttattttcc
      241 gtctgctccg gagtagtcaa tccgtcatct ttcggctgag tttggatctc tacttctaca
      301 ggagtttctc ttggttgagt agtcaataca tcatagttcg actcactttt tgacctagca
      361 tctgttgcta tatctaattt tgggtcgata gaactagttg tataagcttt tggagcttct
      421 agtaggcgtt gtattgcttc aatttgctca tctttaacgt gtaactgatc attcttagct
      481 tttagttcct cacgttggaa ctgctcacgc tcatgagcct gttcaagctg tttttgaagc
      541 atgtaaacct gctgttctaa taggtgaact tctgacaact ttcgtgtttc ggttgtctgt
      601 gaactgttta catttctaga aggttcacca aagactcgaa tagcttcgga aagatcaatt
      661 ttaccgtcac tattcttact taaattacct ttattaactt gcttataaat ggcttgacga
      721 gttattccat agaggtttga aagctcaata acagatagtg atttcatctt gtaattttgg
      781 ttgtccatta tgtgaactaa aatacattaa caaacagtca actgctacat acacaatcga
      841 cctttatgcc ttaaagccta aatttttaag atgaggaata agttgttttt gttttattgg
      901 atctcgcagc atatctttaa ttttaatagc taagtcgtca taactttcac cttgttgtgc
      961 taggtgagat acttcatgta agcgtgaaag ctgattaccg aacatattaa tttgtgaatc
     1021 tgtcaattta taaaaattat tctgttcgga tttatgctca atactttttg atttttcttt
     1081 aaacgtgaag tgaatgtgtg tgacttttcg acctgtcttt atttgctcgt aagatacatt
     1141 taaagggcta tgttcattga tttgatctac agcagtatca ataacccatc tctttaaatc
     1201 agcagacaat ggatatcttt tccctaactc caacatactt cttagagatt cgatggaaat
     1261 ctctcgtttt cctatggaac gatattggct tagtagctca taaatacgaa tagcatatgc
     1321 gctactcata ccagcaatgt cagatagcga atacttagtg aattcacgag ttagattaga
     1381 aataaacggc aaaatttctt tagaaaaccg aattcctaca acgcttttac tttctaaaaa
     1441 tcgtatggat tgaatccatc tcattttgat tgactcatca tcaatagcta aattaattga
     1501 tctgtcatat aagcggttta caccatcttt tagatctcta taagctgttt tttcatgaac
     1561 cccaagtttt tttaaatcat caatagttac gcgataaatt tcatcatctg taatatcttc
     1621 agcccgacta atcttactaa tagctgctaa gacaatccgt tgttctgttg agctgagttg
     1681 atatgaagcc tcaattactt gatttgattt aactaccagt tcttttttag atgtactcat
     1741 catttttaat tcgataaact aactatgagt aatataattc tctcatagtt agtagtcaat
     1801 ccctcatagt taagtagtca atccctcata gttagtagtc aatccctcat agttagtagt
     1861 caatccctca tagttagtat ctgaaagcct ttctgttaaa gcctttcaga tagcctaaaa
     1921 gatttaaaag atttaaaaat aattaaaaag gaaattttaa aaattgccct atgttttcgc
     1981 tacgctcaaa ctctattgaa cttcgcttac gctcagttcg aaggggcaat ttttgggtta
     2041 atctcgtgat aacttcaaaa aaagcaaaag cagctcggaa ttcgcttcgc tcataagttt
     2101 ttttatgctc gcttcgctcg gtctagaagc acattcaatc tgttcgcacc ctacgggatg
     2161 ctctgcacct aaatctctac agcaaagggg agcttgtaat cagggaaaaa acatggcgaa
     2221 aattttaatt gtgtctgttc gctcgtagac actcgctctg taaggcctga ataaaaccag
     2281 ataattttaa aaacctggac caccgtaatc ataatcatca gattttggct ttggctgttc
     2341 gggttgaacc gattgagggg gcttattctg catgggtaat ggatactttg gctgggaatg
     2401 cacttcaaac tcaaagcatg gcgttgagtt atgctctgaa tctctcaatt ttcgattcat
     2461 gttctgaagg ttccagcctt cttgatgcaa tataggctct tgggttttga taatttcact
     2521 aaaatctttt tccagttttt ccttattttt ctggttcttc actaaataat tcaggtaata
     2581 catcacttcc aatgatgaaa atggatggtt ttcagcacta taaaaatatt catgttgttg
     2641 atctaattgc ttttggcgtt cttcgagttt tcgttcttgc tggttgtgtt ctctgatttt
     2701 ttggatttgg gcgagatatt ccgctgtcgc attttgttgg ccaacgccta gttttggctc
     2761 tggaaggtat agcgtgccgt ggatctcgtt atatttttca tagctgtctg ctgaaacctg
     2821 atttttgatg gttatgtctt ggccatccag tgtgtaggtg acggttggac agtgctgtgc
     2881 aagggcatca ttcagatggt tttcccattg ttttcggata taaattaaaa aagtggaata
     2941 gttttcatag ccatcttgca gttggatttt tttggcaccg cctttggctg gatcttgagg
     3001 gttatagcgt ttgaagtact gttctgcgct gcgttcaatg ccgtcatccg cttttagcga
     3061 gaacatcagg tgacaatgag gctgatcttc gccactgatg cgtgatttta cgttgtgtat
     3121 tgcgtatgag tgcggatatt gctgtggaac tatatgtttg tctaaaaacg tctctatgag
     3181 cgttttacgc tgttctggcg tgaactcatt tggtagggca atttcatact ccatgtaggc
     3241 cttggcattg gctcgttcat gactatcggc tgcctgccaa aactcaatcg gatctttgac
     3301 aaaggcgggc atgcaacgtc caggttcaac atgttctagg acttcttcgc tttttttcct
     3361 gatgccttcg tactgcatca accgagcaat atagaggtaa tgctgggtgg cgtagccttt
     3421 attcttgctt ttgatgctga aatggtaggt tgccatgttc acctctgatg gtttgagacc
     3481 ggtttttatg gagtgaaacg agatgaaaac cgcacggatt tggaatgcct ggatggctga
     3541 aaaatccggt tgaagggggt caagggggaa gcgccccatt gcgtactagt ttctccgatt
     3601 aggagaaact gtcaagtgcg aattatttaa aataaaaata aaaattcttt tcaagaaatt
     3661 ttatttttat tttaaataat tattttcaga ctgccctctt caaatatggt aaaaccatat
     3721 ttgaagaggg cagtctgaaa atttggcgac agatgatgga aatggaaaaa gcaaaaaagc
     3781 gtggtcgacc agctcagtta cttcagagag cagagttaca tgctttcgtg gattttttat
     3841 tagagaacaa tcgacgtact gaattacaga atcaggtcat tgatgcattg gaggctgaag
     3901 actttaattt tgagatgttg tctgaagcac agaaaatatt agtcaaagaa acattaaaac
     3961 cttacagaga gcatctgaag cttcagctgc tgtttgatga gctgtctaaa aatcctagac
     4021 ggaccgaata tgaagctaaa tttatcgagt tgtttcatgc ataccaggac aatcaactgg
     4081 gtattgctga actgaatatt ctgaagacga tgtgtacccg atatctgaat ttcaaagctc
     4141 aaaaactgca atatgctgat ttagagctgt atttgagtca gatccagaaa aaggatgctg
     4201 ggaaaaagcg taaagctgaa aacaatcgaa agtatcagct cggtggcgct gtgattgctg
     4261 cttataaaga attgggtctt aatattgata ccttgacacc agatcaagtt agaaaagcca
     4321 ttgtaggaaa taagaggatc atcaataaga tcgaaaaatc tataatattt gagcagattg
     4381 atatggatct tgatactttg tacttcaggt ggccaatatt tctgaaggtc attgataaat
     4441 tagcaatgat tgagtatgag ggtgaagtcg ttcctgagta tataaaagaa atttatttag
     4501 ctttatcaga gtgtaaggca ggcaaaaaat agttaaaaat tattaaattt tagtaataaa
     4561 aagcctgata tatcaggctt atcagtgatt gattggattt agatatccat tttcttaaac
     4621 aagaagaagg gtaaattccg aataatcatg atgatgattg ccaatttgcc aagtgtgtag
     4681 aatattgcaa taaaaaaacc ccggtatcct gccaggggtt tatagcgttt atgcgggttt
     4741 caagccacaa aagagtaaag acgattcata actcaatagt caaagttgaa gaagtgttct
     4801 gggaaacaag aaaggaaggc tttttgaatg tcgctgtatt caccttctgg atgttcctct
     4861 agataacgtc gagtccaaat gactgttgct tcatagtcgt taaagctcgg gtcgagttgt
     4921 aaaaaaatcc agagttcatg tgctttttga atgaaaaagt cattgatgat catgagggca
     4981 attttctttt cagcgtttga attagggtat gagtacatag tatgtctcct atagagatga
     5041 taaatagttt cgttgataaa ctatttataa cacataaaat aatgtgttta ccacataatt
     5101 ttatgtgttt ttatgtaaat ataaccctat gctataatct caaaacacga tttctataaa
     5161 tttaatcaaa tgccatatac tgaatttcaa cgtcttatcg gtaaagcagg gctttcaatt
     5221 aaggaatttg ctgccttatt ggatatgaaa cccaattcga ttacgaatta cagtaaacaa
     5281 ggtgtggttc ctacacatat cgctgtaatc attgcgttga tttctacgat gaaagatgag
     5341 ggattagatt tctatccaat ttttgaaaaa attaagtcgt attcaaaaga gtaaaactct
     5401 ctttttttga tagttgtaag ctttttagta gcgattgatg acgtaaaaaa tgccttaact
     5461 tcaggcattt ttttattttg aggtacttat aaacctcaat agaacttttc aataaagttt
     5521 cgagttttga gaaccagaat gttcatcaaa gtaccttacg ttctgagatc ttaggacgag
     5581 caatcgataa accggacatt tctattttgg agttacaccc gaactgcgca taagagattt
     5641 tatgttaaat atggaagcaa gtcttaatca taagtaacgt catggactac aaacttatta
     5701 ttctttcaat atctattatc tttggattct tctcagctgg ggcatggttt tattcaagcc
     5761 aggttaaagt cactagagaa aaagctgtaa agatagccaa gaaaaaagct caaaagaaaa
     5821 atgaacaacc taatttatca ggagtttctt ttgatggatg ggatgtgaga gaaactttaa
     5881 aagcccaatc aaaatggcag ttacacagaa ttcgggatgg gctcacaacc gcctatgcgt
     5941 atttgacatc aagctcaaaa aaataaccga tttaaaatcg gttatttttg aatggacaat
     6001 cgcaagaatt agcgtgctga acagctttta taaataatct cactagatgg actttgtacc
     6061 tgaatgtcag cacgatattt tttactcgta atacctgcgg tatgtaaact aaagttatta
     6121 tcatcaccaa aagtggtacc aaccatatca ctaagattta gattgattgg cataaagcga
     6181 gttttaccgc ttaagtatgc ttgagcgtaa gtcggtaaac cttgcttatt aaaaccataa
     6241 gtaactttca aacgttgtcc atgtccgcag ttataattaa ctgcttttct aactacagta
     6301 gattcatcca ttttcctagc atcttcttgg ggtctatcac ttgctactgc caaaaatgag
     6361 atgctagtta atacggggat tgcgattaaa gaaaaaattt ttttcattct atgcctctaa
     6421 gaatatttag tttgaagtat tatattatat cataaaccat atcgctaagc ctgagtctca
     6481 cgattcgcta taatccagta gtatttttga aatcaattta ttatcgttct atacctctat
     6541 attcttgagg ctctaatttc ttttcgattt ccctaataac ttggtgagta aatcgttctg
     6601 ctgtgcgttg atgttcgcca gttgcttgtt ctcgttgttt aatctctcgt tctgttctat
     6661 caaattgcga ttgaccgcag tcaatcgctc tatttgttcg ctctgcttgg tggtggtcgc
     6721 ctgtaactcg tttaactgtt gctctaagtc tgtcgctaaa ctgttgaagg cgttcataaa
     6781 gtcgttttgc atggtcgatt attccagttt tagggtgggt ttgccgttgc tttctagctt
     6841 ccctgattct gatttcttgg ggttcttggc tgttatccaa attgatttgt ctttgttttg
     6901 cgtaatcgta actttggcga aagcttgggc ttgtagggaa gtcatctcga tgttctgctg
     6961 tatctctgct atttcttcgt ggtaattgcg tatagtcttg gctaaataaa ggctgactat
     7021 ccccaaggca tggcttttag tcatgctttt tttatcaaaa gagtatagct gaatgataac
     7081 gagccaaata acataaacta tctatcatta aacttaattc aataattaaa tgatagtgtt
     7141 atcattaatc taatagaatt aagattaaat gataaaaaat gaatacacgt atttatctac
     7201 gagcttcaac taaagatcaa gatgcagaaa gggctttgca gattcttcag gatctaaacc
     7261 aaaacttgaa tttgggcgaa accattgtgt acgtggaaaa ctatagtggt acgaagttag
     7321 accgacctga attgaataag ctactgtcac aagcaaatca aggtgacacc ttgttggttg
     7381 aaagtattga ccgattatca cgcctaaccc aacaggattt tcaagagctg aagcgcagga
     7441 tccaggagaa gggacttcgt ttagtcgtag cagatcttcc tactacctac caaatgattc
     7501 aaaccagtga cagcattact cattcaatct tggaactcat caacaatatg ttgattgatc
     7561 tcctggcaac aatggcccgt ctagacaatg agaaacgtat agaacgtatt aagcagggcc
     7621 tagaacgttc gggttacaag ccaacaggta agaaagccaa tgaggctaag cacaaacgaa
     7681 taaaagaatt gctagcatct ggcaatatga ctaaggaaga aattgccaaa gcagtgaatt
     7741 gtggagttgc aactgtctat cgagttgcta aagtcattta aaaaggcttg tatcagtgct
     7801 tcccttatgt catatctatt tacaaaggag caaggcattc ttaaagatca aaagacggaa
     7861 ttaatgatga aaaagcattt gattttcctt gtttttatag tgatggtttt tattttagct
     7921 ctagttgtaa agattaataa tgatcataaa aaagcgcaag cattaaacaa attggaacta
     7981 gttgaaaaga aagctaatta actaaaaatt gacttatact tatttaaata acgctaataa
     8041 ctaagcccaa aaaacaccta gacccagtgc tatacacgct tagttttttt atatttcaaa
     8101 agagtatttt agcaaatatc ataaaagacc ggtctattct taagccggtt ttttatatcg
     8161 cagatcatca tggcttcatc atagatatat gaatattaag ataaataaaa agccctatag
     8221 tctctcccgt tactataagg cttataaaac gctgtaattt ttttcttaat tacctacttt
     8281 tcgatgtagg ttataaatat attttaccta ttggtaaaaa taaagatata agacaaaaga
     8341 tgaatacgtt aggaattccg cgtaaaaact actatgtaac tccaaaatag aactgttctc
     8401 gataaataat gagctaatta acagtacata ttcggctcaa aatatgagtc gaatttgtgc
     8461 tatatttaac tcatcttaga ttttagatga gagagattga aaatgcagaa atggatttgg
     8521 caagcagaaa actggacaaa tttcacatgg caagattcaa ctatcttacc taaaactaga
     8581 cagatacatc aaaaaatagg aattcttctt ggacaaagtc agcatgattt agcgaaagaa
     8641 caattcactc tagatactct acttgccaat ctcgttgctt catcagcaat tgaaaatgaa
     8701 accctcaatg ttttttcatt gcgatcatct ttagcccaac gcttgggtgt cactcttgaa
     8761 cagccttatc caagctcaga tcgatcagaa ggtttagcca atatcatgct ggatgcgctg
     8821 aacgatgtta agcaaccact gactgttgag cgcctcatgc aatggcatcg ttggcttttc
     8881 aatgaccccg actggacaat gcaacgttta cgcattggtc aactgagggg atcagagcct
     8941 atgcaggtcg tttcaggtcg attggattac cctaaagtac attttgaagc accgccaaga
     9001 gaaggcttag aagaacgctt acataccttc atagcgtggt ttaatcagag tctgaatgat
     9061 tcattgcttg atcctctgtt acgtgcaggc attacccatt tatggtttgt taccctacat
     9121 ccttttgatg atggtaatgg ccgtattaca cgtacactta cagaccttgc tctagctcaa
     9181 atggatgagc aaagtatccg tttgtatgca atgtctactg cgatattaaa taaacgtaaa
     9241 agttactatg agatattaga acaaactcaa aaaaatgatt ctgatatcac agattggctg
     9301 atttggtttc tagacacgtt gaatgaaagc cttgaaaaaa ccttagcgca gatcagtcga
     9361 acattgttta aaagtcaatt ttggcataaa tattcaaatt tagccctggg tgaagaacaa
     9421 cgtaaagttc taaatcgttt attagacggt ggcgaaaatg gctttgaaca tggtattagt
     9481 gcttcgcaat atcaaaaggt tgctaaagtc agtaaggcaa cggctactcg ccacttatca
     9541 gacttacttg agaaagaatg tatcgttaaa ttagaaggtg gtggacgtaa tacacgctat
     9601 cagatcaata cccagttata gatattctaa gcaataaaaa accccggcat cctgccgggg
     9661 tttttcgtat tggttcgctc ggtataactc ctgtcctacc ttacagtcct tgtgcttatc
     9721 tattgttatt cttatatgga acttcctgtt cccgatgagt tcataatagt aaaattccag
     9781 tattccggat agcagcaaag agccgagatt gatgtgagcc aaaacgtaca aaatagccta
     9841 aattttatcc acaaaaaatg tggataattt taagcattca agcattgata aaatttaaaa
     9901 aatcgcaaaa tcgactactt tttaaacaaa tttattggca agaactatca aaactgctca
     9961 taaaaaagcc gacttgtttc aaagtcggct tttttatgtt ttttagattt ttatcattta
    10021 aataagttat tgatatttca attaatatac aagtgtactt cgtataaccg ccattatgtt
    10081 aaatagagat aagacatact ctaattttag agttttcttt taggtatgat ataaaaatat
    10141 acaagcctaa cagtttagtt taaaggttaa aaattgaata aattaacaaa tatttttatg
    10201 gctttaggag tttgcctttc aatggaaaca tttgcagaac ctgttttttc taatgattta
    10261 ttagcgaaag cagaaaatgg agatacttct gctcagttag aattagccga gatttatcta
    10321 tatggtcatg gtgttgattc agatgaaaat caagctgaag tttgggctct taaatcagca
    10381 gaaaatggaa atgtagcagc aatgttttgg ttagctgatg gatatgttac ttatgctaga
    10441 ttaatggaag atgatgacaa aaacgattct ttagaacatt tccaaaaagc ttttaagtgg
    10501 tttcagaaag cctcagaaaa tggccattct gaatcaatgg ttgagttagc tgatctatat
    10561 actcgtgcag atagcggaat agaagttaat attaataaag cttttgaact tcgtgaaaag
    10621 gctgcaaagt taggtaataa gaaagcaatg cgaagtcttt ccgttatgta tcgtgatggt
    10681 ataggtattc ctaaaaatac tgatttagct caaagttggt gggataagtc tgaaaattag
    10741 ttttaatatt tcctaaaatt aacataatac accttatacg aaatgc
//
