LOCUS       NZ_AP025279             5220 bp    DNA     circular CON 08-FEB-2024
DEFINITION  Aeromonas hydrophila strain NUITM-VA1 plasmid pNUITM-VA1_2, complete
            sequence.
ACCESSION   NZ_AP025279
VERSION     NZ_AP025279.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00424814
            Assembly: GCF_021654355.1
KEYWORDS    RefSeq.
SOURCE      Aeromonas hydrophila
  ORGANISM  Aeromonas hydrophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Aeromonadales;
            Aeromonadaceae; Aeromonas.
REFERENCE   1
  AUTHORS   Suzuki,M., Dao,T.D. and Takemura,T.
  TITLE     Genomic analysis of Aeromonas hydrophila strain NUITM-VA1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5220)
  AUTHORS   Suzuki,M., Dao,T.D. and Takemura,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-NOV-2021) Contact:Masato Suzuki National Institute of
            Infectious Diseases, AMR Research Center; 4-2-1 Aobacho,
            Higashimurayama, Tokyo 189-0002, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 2.1.1
            Genome Coverage                   :: 192x
            Sequencing Technology             :: ONT MinION;Illumina MinSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/08/2024 03:37:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,733
            CDSs (total)                      :: 4,570
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 163
            rRNAs                             :: 11, 10, 10 (5S, 16S, 23S)
            complete rRNAs                    :: 11, 10, 10 (5S, 16S, 23S)
            tRNAs                             :: 126
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 18 of 84
            Pseudo Genes (incomplete)         :: 68 of 84
            Pseudo Genes (internal stop)      :: 12 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP025279.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5220
                     /organism="Aeromonas hydrophila"
                     /mol_type="genomic DNA"
                     /strain="NUITM-VA1"
                     /db_xref="taxon:644"
                     /plasmid="pNUITM-VA1_2"
                     /geo_loc_name="Viet Nam:Hanoi"
                     /lat_lon="20.99138062 N 105.86277218 E"
                     /collection_date="2021"
     gene            593..898
                     /locus_tag="L6424_RS23585"
                     /old_locus_tag="NUITMVA1_45790"
     CDS             593..898
                     /locus_tag="L6424_RS23585"
                     /old_locus_tag="NUITMVA1_45790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005322034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063639082.1"
                     /translation="MKDARDDQTLDLLPSGKRRGRPPTGKALSPAAKQAAYRARQREKT
                     VTVTLNRPDCGELEIFLLNLRDGRTSTLAPEVVARLHDAVRSAWLGQLHSGNGDQK"
                     /name="hypothetical protein"
     gene            895..1236
                     /gene="mobC"
                     /locus_tag="L6424_RS23590"
                     /old_locus_tag="NUITMVA1_45800"
     CDS             895..1236
                     /gene="mobC"
                     /locus_tag="L6424_RS23590"
                     /old_locus_tag="NUITMVA1_45800"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_063639083.1"
                     /translation="MTPAKRERVIKIRVTDEEFARLKGLSGDERLAEWMRSQCLGHDKA
                     AARRRTPVPKADPALLRQIAGIGNNLNQVARRVNSGEWGAVERVQVIAVLMAMERALQA
                     LSVPSTEVT"
                     /name="mobC"
     gene            1236..1406
                     /locus_tag="L6424_RS23595"
                     /old_locus_tag="NUITMVA1_45810"
     CDS             1236..1406
                     /locus_tag="L6424_RS23595"
                     /old_locus_tag="NUITMVA1_45810"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_164882767.1"
                     /translation="MNEYGVAEYDYTLIRLPGEQGWSLRLLKDGQEVSGEVYQEHDEAL
                     SVATVWLCSES"
                     /name="hypothetical protein"
     gene            1622..>2053
                     /locus_tag="L6424_RS23600"
                     /pseudo=""
     CDS             1622..>2053
                     /locus_tag="L6424_RS23600"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2091..2564
                     /locus_tag="L6424_RS23605"
                     /old_locus_tag="NUITMVA1_45830"
     CDS             2091..2564
                     /locus_tag="L6424_RS23605"
                     /old_locus_tag="NUITMVA1_45830"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_063639085.1"
                     /translation="MSKILELAKTFEESSKQQASDIETSVKNAFEPHERAILAALESSA
                     RRLNTAIDAQNRRWGWLVLKGWVFPLIGVAFLLGISWVVVWYQGRVIAENWVEISRQNK
                     TLEQLAAKGGKLELSTCGKDQRLCVKMDLKELAYGREEESERGEYPWRIPEGY"
                     /name="MbeB family mobilization protein"
     gene            2565..2789
                     /locus_tag="L6424_RS23610"
                     /old_locus_tag="NUITMVA1_45840"
     CDS             2565..2789
                     /locus_tag="L6424_RS23610"
                     /old_locus_tag="NUITMVA1_45840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004577252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_063639086.1"
                     /translation="MTELERQLLKALESLEGGYRAQHAAWESAYSDLRQMFEATSTENR
                     ALRESVGSLSKQVLHLSGQVETLSQKLKP"
                     /name="MbeD family mobilization/exclusion protein"
     gene            3049..4293
                     /locus_tag="L6424_RS23615"
                     /old_locus_tag="NUITMVA1_45860"
     CDS             3049..4293
                     /locus_tag="L6424_RS23615"
                     /old_locus_tag="NUITMVA1_45860"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]; GO:0046872 - metal ion binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="WP_236766889.1"
                     /translation="MVKRIPVSELRLGMYIHKLAGSWFRHPFWRGSFLLTEPQDLSAIR
                     ECGVGEVWVDLAKSQVDPESPESPESPESPESPESPEPRELSEEQSLPSSPLSKKSDGA
                     TSMESEMCYARKLCLAAKSQVMDMFQEARLGKAVDPSTTLPLVGEIAASVLRQPHALIS
                     VARIKTHDDYTYLHSVAVCALMLSLARHLDLDEEQTRLAGIGGLMHDLGKAAMPLEVLN
                     KPGKLTDAEFAIMKRHPVEGAKMLRAGGAEPGVVDIALHHHEKIDGTGYPDRLAGDAIS
                     LLARMGAICDVYDAVTSERAYKKPWDPSAAMRQMAKWEGHFDKRIFHAFVKAVGIYPVG
                     SLVRLSSQRLAVVVEPGMESLLTPKVRVFFSLRSREPIPMQTIDLAATSCKDSITGPED
                     PTLWNFKNLDDLWME"
                     /name="HD-GYP domain-containing protein"
     Typing          1404..2869
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.82"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cggcgcggca gcccgaccaa caggcggggg ctaaagcaga gtgcagcggg acggggtgtg
       61 ttctctggag cgcgtgagag ggagctgccg caagcagtct cgatagtccg gagcaaggcg
      121 agcttatcct gtaaaagacc tcaaatcgat atgggtctgt aggagggctc tcctatcttg
      181 aagcgcagcg aaagatagga gagcccggca aactgtggcg gcttgccaac atccttttct
      241 cccgtttttt gtttccccag ggcacgtatc atcaccccct cgttgagctc tttttgcctc
      301 ctaccggtta cctaccctcg aagccctcta attatagtca ctgttacttt aactagtctc
      361 gccaacaggt ggttacaacc tattccggct atgtcaaact tgactctaag ccagcataca
      421 cagagggccc ttagccgctt cgcgtcttcg gttcctctgt gaagctggtt caggggcgtg
      481 ccgcccccga aaccccctgc ctccaccgcc ccgaaaaacc ccgttccggg ctttcccagg
      541 tcggcggaca ggataggtat atggtcacag ttaccgtaat tgaggtgatc ggatgaagga
      601 tgcacgagac gaccagacgc tggacttgtt gccgagtggc aagcgccggg gtcggccacc
      661 taccggcaag gcgttgagcc ctgccgccaa gcaagctgct taccgggcac ggcaacggga
      721 aaaaacggtc accgttacct taaatcgtcc ggattgtggc gagctggaga tcttcctgct
      781 gaacctacgg gatggccgaa cctcgacctt ggcccccgag gtggtggccc ggttgcatga
      841 tgctgtgcgg tcggcgtggc tggggcagct tcattccggt aacggtgacc agaaatgacc
      901 ccggccaaac gtgaacgggt gatcaagatc cgagtcaccg acgaggaatt tgcccggctc
      961 aagggcttga gtggcgatga acggctggcc gaatggatgc ggagccagtg tcttgggcac
     1021 gacaaggccg ccgctcgacg gcggacaccg gtacccaaag ccgatccggc tctgctgagg
     1081 cagatagccg ggatcggcaa caacctcaac caggtagcgc ggcgagtgaa tagcggggag
     1141 tggggtgcgg ttgaacgtgt ccaggtgatc gctgtgctga tggcgatgga gcgtgctttg
     1201 caggccctgt ctgtcccctc aacggaggta acgtgatgaa cgaatacggc gtagccgagt
     1261 atgactacac cctgatccgg ttgcccggcg aacaggggtg gagcttgcgg ttgttgaagg
     1321 acggccagga ggtcagcggg gaggtttacc aggagcacga tgaagcgctc agtgtggcca
     1381 ccgtctggct gtgcagcgag tcatgatcgt caaatttcat gcccgtggtg tggggcgcgg
     1441 tagcggcccg gtcgattacc tgctggggcc gcagcgggat cgggaggggg cgacccttga
     1501 ccgtggcgat ccgggtctca tcgaggcgct gatcgacagc agcccctacg ccaaaaagta
     1561 cacctctggc gtgctgtcgt tccaggaagc ggatttgccc agggaggaca aggacaagct
     1621 gatggacagc ttcgagcgtg cgttattgcc ggggctggat cgggatcagt acgcctgcct
     1681 gtgggtggag caccgggaca aagaacggct tgagctcaac ttcgtgatcc cgaatatcga
     1741 actgctgagt ggcaagcggc tgcaacccta tttcgaccgg gccgaccggc cccgcatcga
     1801 tgcgtggaag gtggtggtca acgcccagtt gggcttgcat gacccggatg atcccttgaa
     1861 caaacgggca ttgatgaccc ccagcaactt gccaaaagcc aaacaggacg cggcacaagc
     1921 gatcaccgac ggtctgcttt cactggcggc ggccggtgag ctggccgacc ggggagacgt
     1981 ggtggccacg ttgaagcaat acggctttga ggtggtgcgg gagacccgca agagtatcag
     2041 cattgccgac ccggaagggg ggcggaatat ccggctaaaa ggggccctgt atgagcaaga
     2101 ttttagagct ggccaagacc ttcgaagaga gctcgaagca gcaggcgagc gatatcgaaa
     2161 cgagcgtgaa gaacgcgttc gagccgcacg agagagctat tctcgcggcg ttggaatcaa
     2221 gcgcgcggag attgaacacc gctatcgacg cccagaaccg gaggtggggc tggctggtgt
     2281 tgaaggggtg ggtctttccg ttgatcgggg tggcgtttct gctggggatc agctgggtcg
     2341 tggtgtggta ccagggcagg gtcatcgccg agaactgggt agagatctca cgccagaaca
     2401 agacattgga gcagttggca gcgaagggcg gaaaattgga actgtcgacg tgcggcaagg
     2461 accagcggct gtgcgtgaag atggacttga aggagttggc ctatggccgc gaagaggaat
     2521 cagagagagg ggaatatccc tggaggatcc cggaggggta ctgaatgacg gaactagagc
     2581 ggcagttatt gaaagcgtta gagagcttgg aaggcgggta cagagcgcag cacgcggcct
     2641 gggagagcgc ctacagcgat ttgcggcaga tgttcgaggc cacctctacc gagaaccggg
     2701 cattgcgaga gagcgtgggg agcttgagca agcaggtgtt gcacttgagc ggtcaagtcg
     2761 agacattgag ccaaaaattg aagccctgaa cattgagctg gagccgcggc gtagctctat
     2821 gagcctcggg cgggagtatc gagagtatgg tgacagggag tacggtgatt gagtggggtc
     2881 agtgggcccc acttcattgt tgatgcccct gttaggggtt gtacgccgga atcccaattt
     2941 cggcggttcc ggctgatcca agaatcttgg cgtgacctta cctccgctct accgctgggt
     3001 gccagcctcg gcgcacgcct aaccacccaa ctcagaaagg aaaataagtt ggtaaaacgc
     3061 attcccgtat ccgagcttcg tctcggcatg tacatccaca agctcgccgg ctcttggttt
     3121 cgacacccat tctggcgcgg cagcttcctg ctgaccgagc ctcaggatct ctctgccatt
     3181 cgagaatgtg gcgtcgggga ggtctgggtc gacttggcca aaagccaagt cgacccagag
     3241 agcccagaga gcccagagag cccagagagc ccagagagcc cagagagccc agagcccaga
     3301 gaattgtcgg aggagcaatc tctgccgtct agtccactaa gcaagaaaag tgacggcgca
     3361 acctcaatgg agagcgaaat gtgttatgca cggaaactct gtcttgcggc caagtcccaa
     3421 gtcatggaca tgttccagga agcccggctt ggcaaggccg tcgacccaag cacgacgttg
     3481 ccgctggtcg gagaaatcgc tgcctcggtg ctgcgccaac ctcatgccct catcagcgtc
     3541 gcacgcatca aaacgcacga cgattacacc tacctgcact cggttgccgt ctgcgccctg
     3601 atgctatcgc tggcccggca tctcgatcta gacgaggagc aaacgcgcct ggctggcatc
     3661 ggcggactga tgcacgacct aggcaaggcc gcgatgccgc tggaagtgct taacaaacca
     3721 ggcaagctca ccgatgccga gttcgccatc atgaagcgcc accctgtgga gggcgcaaag
     3781 atgctgcgcg caggcggggc cgagcccggg gtggtggaca ttgccctgca ccatcacgag
     3841 aagatcgacg gaaccggcta cccggatcgc ttggccggtg acgccatttc actcctggcc
     3901 cgcatgggcg caatctgcga cgtctatgat gccgtgacgt cggagcgagc ctacaaaaag
     3961 ccgtgggacc cgtccgcggc gatgcggcag atggccaagt gggagggcca tttcgacaaa
     4021 cgcatcttcc acgccttcgt caaggccgtg ggcatctacc ccgtcggctc cttggttcgc
     4081 ctgtcctctc agcgtctggc cgttgtcgtt gagccgggaa tggaatcact gctgactccc
     4141 aaggttcgcg tgttcttctc gctacgctcg agagagccga tcccgatgca gaccatcgac
     4201 ctggcggcca cgagttgcaa ggacagtatc actggccccg aagacccgac gctctggaac
     4261 ttcaagaacc tcgacgactt gtggatggaa tagcccccac aaaacgacgg ccccgtgagg
     4321 gagccgttga acatcgtcga gcgatgcccg tccagggagg gatggccgag ctcgattctc
     4381 cagaaggtag ctggtgacac gagtgttgag cccacccaac tagggtgacg gaaatttctg
     4441 gggttccggc ttacatcccc tgttacccca tgataaggat ttagttagtt ggccacattt
     4501 tggctgaacg ttgtgagcta agaggtaagt ctttatttgt taaggataaa aaatatagac
     4561 cgaaggcatg gccagaataa gtgctggacc tcctttagct tgagacggct gtatcggctg
     4621 gggggactgt tgcgcggcgt agaactccga taacgaatat gccgtaatca agacaggatc
     4681 cttttccatc tcttcggatc cttcattaaa acgcgctatg gcgcgttttt tgttcctgct
     4741 atggaaatgc tatgggttag gtggcgagtg agctctacgc tcgttctgtg aggttcgtat
     4801 tctaaatgat cttgatcaga tcctctgatc aagcgtaacc tgcccaccag aaaacaaaaa
     4861 acccacctat ctaggcgggt tctttgcccg gtgttcctcg gcttgccggc cttggaatcc
     4921 ggtgggtaac agcttcaagc aggaagcgga taccgttaag taccccctca gtttagccgc
     4981 gtgcggccca ctgttcaagt atcaaacccc tgctcgaacc ctgttaccaa aggctttcac
     5041 ctttggcgct ttgtcgtatt cgctcggatt ggacgcaagt gattgttgcc gtagcgggtg
     5101 tgaaggtcgg gcagaacggg gaaatctcac atactgccca agcgtgcaat cgcgcaccac
     5161 agcctcccag gactcgcaac cctggggcat ctgcaagtag agcgtaacgg ggaaccaacc
//
