LOCUS       NZ_AP025278             8138 bp    DNA     circular CON 08-FEB-2024
DEFINITION  Aeromonas hydrophila strain NUITM-VA1 plasmid pNUITM-VA1_1, complete
            sequence.
ACCESSION   NZ_AP025278
VERSION     NZ_AP025278.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00424814
            Assembly: GCF_021654355.1
KEYWORDS    RefSeq.
SOURCE      Aeromonas hydrophila
  ORGANISM  Aeromonas hydrophila
            Bacteria; Pseudomonadota; Gammaproteobacteria; Aeromonadales;
            Aeromonadaceae; Aeromonas.
REFERENCE   1
  AUTHORS   Suzuki,M., Dao,T.D. and Takemura,T.
  TITLE     Genomic analysis of Aeromonas hydrophila strain NUITM-VA1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8138)
  AUTHORS   Suzuki,M., Dao,T.D. and Takemura,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-NOV-2021) Contact:Masato Suzuki National Institute of
            Infectious Diseases, AMR Research Center; 4-2-1 Aobacho,
            Higashimurayama, Tokyo 189-0002, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 2.1.1
            Genome Coverage                   :: 192x
            Sequencing Technology             :: ONT MinION;Illumina MinSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/08/2024 03:37:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,733
            CDSs (total)                      :: 4,570
            Genes (coding)                    :: 4,486
            CDSs (with protein)               :: 4,486
            Genes (RNA)                       :: 163
            rRNAs                             :: 11, 10, 10 (5S, 16S, 23S)
            complete rRNAs                    :: 11, 10, 10 (5S, 16S, 23S)
            tRNAs                             :: 126
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 18 of 84
            Pseudo Genes (incomplete)         :: 68 of 84
            Pseudo Genes (internal stop)      :: 12 of 84
            Pseudo Genes (multiple problems)  :: 12 of 84
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP025278.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8138
                     /organism="Aeromonas hydrophila"
                     /mol_type="genomic DNA"
                     /strain="NUITM-VA1"
                     /db_xref="taxon:644"
                     /plasmid="pNUITM-VA1_1"
                     /geo_loc_name="Viet Nam:Hanoi"
                     /lat_lon="20.99138062 N 105.86277218 E"
                     /collection_date="2021"
     gene            join(7313..8138,1..14)
                     /locus_tag="L6424_RS23520"
     CDS             join(7313..8138,1..14)
                     /locus_tag="L6424_RS23520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="WP_094337857.1"
                     /translation="MATHKPINILEAFTAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVLYLPAEDPPTAIHHRLHALGAYLNDEQRQAVAA
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
                     /name="helicase RepA family protein"
     gene            1..852
                     /gene="repC"
                     /locus_tag="L6424_RS23525"
                     /old_locus_tag="NUITMVA1_45690"
     CDS             1..852
                     /gene="repC"
                     /locus_tag="L6424_RS23525"
                     /old_locus_tag="NUITMVA1_45690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="WP_000240536.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
                     /name="repC"
     gene            1147..>1563
                     /locus_tag="L6424_RS23530"
                     /old_locus_tag="NUITMVA1_45700"
                     /pseudo=""
     CDS             1147..>1563
                     /locus_tag="L6424_RS23530"
                     /old_locus_tag="NUITMVA1_45700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /name="dihydropteroate synthase"
     gene            1921..2943
                     /locus_tag="L6424_RS23535"
                     /old_locus_tag="NUITMVA1_45710"
     CDS             1921..2943
                     /locus_tag="L6424_RS23535"
                     /old_locus_tag="NUITMVA1_45710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821153.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA]; GO:0008324 - monoatomic cation
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="WP_000083351.1"
                     /translation="MSQSHAHDHGHDHDHDHTPTVTSANERKVLVSFFLIFGFMLVEAV
                     GGVLSGSLALLADAGHMLTDAVALALAYAAFRFGRRAADSKRTFGYLRFEVIAGFLNAV
                     TLFAIVAWIAYEAWERLQAPPVILAGPMMIVAVLGLLINVLVLWIMTRGETDHVNVKGA
                     ILHVMGDLLGSVGAIVAAIVIYFTGWTPIDPILSVLVAALILRSAWKLLAKSIHILLEG
                     APEDASPEKVEQGLMSTVPGLAAVSHVHVWQLTSGRTMATLHVRPNVDEEARAVVKRVE
                     VVLREQFSIEHATVGIDWNSEADENICSLQPTTDRQDHSGHGHSEHNHDHDHSAPGHKH
                     "
                     /name="cation diffusion facilitator family transporter"
     gene            2987..3364
                     /locus_tag="L6424_RS23540"
                     /old_locus_tag="NUITMVA1_45720"
     CDS             2987..3364
                     /locus_tag="L6424_RS23540"
                     /old_locus_tag="NUITMVA1_45720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821152.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_000203935.1"
                     /translation="MTSRKTTSSAIAHCSTSSHGATTDVLALSQSDVAVLAETFRLLGD
                     QSRLKILLQCMRGSVAVGDIADSLDLSQSLVSHHLRLLRGARLVRGERQAKHIFYGIAD
                     QHVSQVLQDMAVHISEDRNDD"
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            complement(<3469..3630)
                     /locus_tag="L6424_RS23545"
                     /old_locus_tag="NUITMVA1_45730"
                     /pseudo=""
     CDS             complement(<3469..3630)
                     /locus_tag="L6424_RS23545"
                     /old_locus_tag="NUITMVA1_45730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(4230..4397)
                     /locus_tag="L6424_RS23550"
                     /old_locus_tag="NUITMVA1_45740"
     CDS             complement(4230..4397)
                     /locus_tag="L6424_RS23550"
                     /old_locus_tag="NUITMVA1_45740"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_161403145.1"
                     /translation="MTDLKLDDYTDADFIDELLSDELRCECGEELHYYDDGSLSRCNSC
                     DPDRETNPNL"
                     /name="hypothetical protein"
     gene            complement(4424..4708)
                     /locus_tag="L6424_RS23555"
                     /old_locus_tag="NUITMVA1_45750"
     CDS             complement(4424..4708)
                     /locus_tag="L6424_RS23555"
                     /old_locus_tag="NUITMVA1_45750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WP_094336938.1"
                     /translation="MAKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPA"
                     /name="mobilization protein"
     gene            5013..>5501
                     /locus_tag="L6424_RS23560"
                     /pseudo=""
     CDS             5013..>5501
                     /locus_tag="L6424_RS23560"
                     /inference="COORDINATES: protein motif:HMM:NF015354.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /name="MobA/MobL family protein"
     gene            complement(5448..5642)
                     /locus_tag="L6424_RS23565"
     CDS             complement(5448..5642)
                     /locus_tag="L6424_RS23565"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236766888.1"
                     /translation="MVYSLPVFLQVDDLGVGGVDVQGHVYAPAFHLHHVRPQVNTGQAL
                     DALRLKRSVVNAGVVASPL"
                     /name="hypothetical protein"
     gene            5641..6054
                     /locus_tag="L6424_RS23570"
     CDS             5641..6054
                     /locus_tag="L6424_RS23570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncQ-type mobilization protein MobB"
                     /protein_id="WP_223384840.1"
                     /translation="MSAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQAS
                     EAQAAEWLKAQRETQAAWAKLAKELREVASEVSDAAQSARSAARGWHWKLWLTVMAASM
                     MPTLVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
                     /name="IncQ-type mobilization protein MobB"
     gene            6051..7022
                     /locus_tag="L6424_RS23575"
     CDS             6051..7022
                     /locus_tag="L6424_RS23575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-primase RepB domain-containing protein"
                     /protein_id="WP_236766887.1"
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSASEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSELDLEDMKAEGREPALIVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRTGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRYGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELAQAPAPRQRGMDRGGPDFSM"
                     /name="DNA-primase RepB domain-containing protein"
     gene            7078..7284
                     /gene="urfF"
                     /locus_tag="L6424_RS23580"
                     /old_locus_tag="NUITMVA1_45770"
     CDS             7078..7284
                     /gene="urfF"
                     /locus_tag="L6424_RS23580"
                     /old_locus_tag="NUITMVA1_45770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid repression protein F"
                     /protein_id="WP_094336940.1"
                     /translation="MKDHRDTQTGNLLATPNAIRQARFADAQRKLGRKARKIWATDDEA
                     EALRLYLEELRAAQGGGSDPASA"
                     /name="urfF"
     ARG             1148..1662
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide resistant sul"
                     /tool="rgi"
                     /drug_class="sulfonamide antibiotic"
                     /identity="100.0"
                     /coverage="63.1"
     Typing          4663..4819
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.671"
                     /coverage="100.0"
                     /evalue="1.08e-59"
     Typing          7331..8138
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.775"
                     /coverage="94.0"
                     /evalue="0.0"
     Typing          4909..7019
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.063"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtggtgaagc ctaagaacaa gcacagcctc agccacgtcc ggcacgaccc ggcgcactgt
       61 ctggcccccg gcctgttccg tgccctcaag cggggcgagc gcaagcgcag caagctggac
      121 gtgacgtatg actacggcga cggcaagcgg atcgagttca gcggcccgga gccgctgggc
      181 gctgatgatc tgcgcatcct gcaagggctg gtggccatgg ctgggcctaa tggcctagtg
      241 cttggcccgg aacccaagac cgaaggcgga cggcagctcc ggctgttcct ggaacccaag
      301 tgggaggccg tcaccgctga tgccatggtg gtcaaaggta gctatcgggc gctggcaaag
      361 gaaatcgggg cagaggtcga tagtggtggg gcgctcaagc acatacagga ctgcatcgag
      421 cgcctttgga aggtatccat catcgcccag aatggccgca agcggcaggg gtttcggctg
      481 ctgtcggagt acgccagcga cgaggcggac gggcgcctgt acgtggccct gaaccccttg
      541 atcgcgcagg ccgtcatggg tggcggccag catgtgcgca tcagcatgga cgaggtgcgg
      601 gcgctggaca gcgaaaccgc ccgcctgctg caccagcggc tgtgtggctg gatcgacccc
      661 ggcaaaaccg gcaaggcttc catagatacc ttgtgcggct atgtctggcc gtcagaggcc
      721 agtggttcga ccatgcgcaa gcgccgccag cgggtgcgcg aggcgttgcc ggagctggtc
      781 gcgctgggct ggacggtaac cgagttcgcg gcgggcaagt acgacatcac ccggcccaag
      841 gcggcaggct gacccccccc actctattgt aaacaagaca tttttatctt ttatattcaa
      901 tggcttattt tcctgctaat tggtaatacc atgaaaaata ccatgctcag aaaaggctta
      961 acaatatttt gaaaaattgc ctactgagcg ctgccgcaca gctccatagg ccgctttcct
     1021 ggcttatgct attctgctcc tgcagctaat ggatcaccgc aaacaggtta ctcgcctggg
     1081 gattcccttt cgacccgagc atccgtatga gactcatgct cgattattat tattatagaa
     1141 gcccccatga ataaatcgct catcattttc ggcatcgtca acataacctc ggacagtttc
     1201 tccgatggag gccggtatct ggcgccagac gcagccattg cgcaggcgcg taagctgatg
     1261 gccgaggggg cagatgtgat cgacctcggt ccggcatcca gcaatcccga cgccgcgcct
     1321 gtttcgtccg acacagaaat cgcgcgtatc gcgccggtgc tggacgcgct caaggcagat
     1381 ggcattcccg tctcgctcga cagttatcaa cccgcgacgc aagcctatgc cttgtcgcgt
     1441 ggtgtggcct atctcaatga tattcgcggt tttccagacg ctgcgttcta tccgcaattg
     1501 gcgaaatcat ctgccaaact cgtcgttatg cattcggtgc aagacgggca ggcagatcgg
     1561 cgcggtcatg ggcttaccat cgaccttcat tgtcatgtct ctgatcggct tggccggcgt
     1621 ggtgctggcg ctctggctgt ggccggccag cgacccttct gacgtggtgc atgaccaccc
     1681 cagcttgccg ccggatcacc ctcatttacg cacacacgca gaccgaggca agcaccatca
     1741 ccagctgatt gtggatgacc tgcaccgtat ctggccgaaa ggataggcac attttctcgt
     1801 tgcggcacca caacgtatgg cttcggcggc tgggaagggt gagcgaaaat acttcacatg
     1861 aacatgtatt catgtgttat attttcacca tagtcaaaca ctcaaccagc agaggcgccc
     1921 atgtcccaat cccatgcaca tgaccatggt cacgaccatg atcacgacca cacccccaca
     1981 gtgaccagcg ccaacgagcg caaggttctg gtttccttct tcctgatctt cggcttcatg
     2041 ctcgtggaag ctgtcggcgg cgtgctgtcg ggctcgctag ccctacttgc cgatgctggc
     2101 cacatgctga ccgacgccgt ggcacttgcc ctggcttacg ctgccttccg attcggtcgc
     2161 cgcgcagccg acagcaaacg cacattcggt tacctgcgct tcgaggtcat cgccggcttc
     2221 ctgaacgccg tgaccctgtt cgcgatcgtc gcatggatcg cctacgaggc gtgggagcgg
     2281 ctgcaggcgc cgcccgtcat cctcgccggc ccgatgatga tcgtcgccgt gctcggctta
     2341 ctgatcaacg tcctcgtgct gtggatcatg acccgcggcg aaaccgatca cgtcaatgtg
     2401 aaaggtgcca ttctccacgt gatgggtgac ctgctgggct ctgtcggcgc catcgttgca
     2461 gcgatcgtca tctatttcac cggctggacg ccgatcgacc ccattctgtc ggtcttggtg
     2521 gcagcgttga ttctgcgcag cgcctggaag ctcctggcca agtcgatcca tatcctgctg
     2581 gaaggcgcgc cagaggatgc ttcgccggaa aaggtggaac aaggcctgat gagcactgta
     2641 cccggcctgg cagctgtcag ccacgttcat gtgtggcaac tcacttccgg tcgcacgatg
     2701 gccacactgc acgttcgacc caatgtggac gaggaggcgc gagccgtggt caagcgcgtc
     2761 gaggtggtgc ttcgagagca attcagcatc gaacacgcca cagtcggaat cgactggaac
     2821 tcggaggctg acgagaacat ctgcagcctg caacccacga cagaccgtca ggaccacagc
     2881 ggccatggcc acagtgagca caatcatgac catgaccact ccgctcctgg tcacaagcat
     2941 tgaggccgtg cgagccggct cgcagttcac ttgagggttt gaatccatga catcacgcaa
     3001 aaccaccagc tccgctattg ctcactgttc gacctccagc cacggcgcga ccactgatgt
     3061 cctcgcactg tcgcaaagtg acgtcgctgt tctggccgag accttccgcc tgctgggcga
     3121 ccaatcgcga ttgaaaatcc tgttgcagtg catgcgcgga tccgtcgcag tgggcgacat
     3181 tgccgattcg ctagacctgt cgcaatcctt ggtcagccat cacctgcgcc tgttgcgcgg
     3241 cgcgcgcctc gtgcgcggcg agcgccaggc caagcacatc ttctacggca tcgccgacca
     3301 gcacgtcagc caggtactgc aggacatggc ggttcacatt tcggaggaca gaaacgacga
     3361 ttgactgaac gtgtgagtgc gcgccggcct actccttcaa ggggtaggct tttttttggc
     3421 tatcaatcta gagaaacacc gacgcgcctt gctgaatgcc tagaacccca catagcccgg
     3481 caattccttt ccctgctctg ccataagcgc agcgaatgcc gggtaatact cgtcaacgat
     3541 ctgatagaga agggtttgct cgggtcggtg gctctggtaa cgaccagtat cccgatcccg
     3601 gctggccgtc ctggccgcca catgaggcat gttccgcgtc cttgcaatac tgtgtttaca
     3661 tacagtctat cgcttagcgg aaagttcttt taccctcagc cgaaatgcct gccgttgcta
     3721 gacattgcca gccagtgccc gtcactcccg tactaactgt cacgaacccc tgcaataact
     3781 gtcacgcccc cctgcaataa ctgtcacgaa cccctgcaat aactgtcacg cccccaaacc
     3841 tgcaaaccca gcaggggcgg gggctggcgg ggtgttggaa aaatccatcc atgattatct
     3901 aagaataatc cactaggcgc ggttatcagc gcccttgtgg ggcgctgctg cccttgccca
     3961 atatgcccgg ccagaggccg gatagctggt ctattcgctg cgctaggcta cacaccgccc
     4021 caccgctgcg cggcaggggg aaaggcgggc aaagcccgct aaaccccaca ccaaaccccg
     4081 cagaaatacg ctggagcgct tttagccgct ttagcggcct ttccccctac ccgaagggtg
     4141 ggggcgcgtg tgcagccccg cagggcctgt ctcggtcgat cattcagcat cggcatcatc
     4201 tgctaatttc ccgcaatgtt cgcaggtaat cataggtttg gattggtttc tctatctgga
     4261 tcacacgaat tgcagcgact caatgaacca tcatcgtaat aatgaagctc ctctccgcat
     4321 tcacagcgca gttcatcact gagcagttca tcaataaaat cagcgtctgt gtaatcgtct
     4381 agttttaaat cagtcattat tgctttcctt tattagttta aattcaggcc ggctcatcct
     4441 tctggcgtgg cggcagaccg aacaaggccc ggtcgtggtc gcgttcaagg tacgcatcca
     4501 ttgccgccat gagccgatct tctggccact cgctgctgtt caccttggcc agaatcatgg
     4561 cgcccaccag caccttgcgc cgcgtttcgt tcttgcgctc ttgctgctgc tcccttgccc
     4621 tcacccgctg aatctcggca ttgatccgtg ctcgctgttc ttcgagcttg gccagcctat
     4681 ccgccgcctt gttgctcccc ttagccatct tgacacctca ttgttaatgt gctgtctctt
     4741 aggctatcat ggaggcacga cggcggcaaa ccccgaccgt actttgtacg ggagggcgca
     4801 cttaccggtt tctcttcgag aaactggcct aacggccacc cttcgggcgg tgcgctctcc
     4861 gagggccatt gcatggagcc gaaaagcaaa agcaacagcg aggcagcatg gcgatttatc
     4921 accttacggc gaaaaccggc agcaggtcgg gcggccaatc ggccaaggcc aaggccgact
     4981 acatccagcg cgaaggcaag tatgcccgcg acatggatga agtgctgcac gccgaatccg
     5041 ggcacatgcc ggagttcgtc gagcggcccg ccgactactg ggatgctgcc gacctgcatg
     5101 aacgcgccaa tgggcggctg ttcaaggagg tcgaatttgc cctgccggtc gagctgaccc
     5161 tcgaccagca gaaggcgctg gcgtccgagt tcgcccagca cctgaccggt gccgaacgtt
     5221 tgccgtatac gctggccatc catgccggtg gcggcgagaa cccgcactgc cacctgatga
     5281 tctccgagcg gatcaatgac ggcatcgagc ggcccgccgc tcagtggttc aagcggtaca
     5341 acggcaaggc cccggagaag ggcggagcgc agaagaccga agcactcaag cccaaggcat
     5401 ggcttgagca gacccgcgag gcatgggccg accatgccaa ccgggcatta gagcgggctg
     5461 gccacgacgc ccgcattgac caccgaacgc ttgaggcgca gggcatcgag cgcctgcccg
     5521 gtgttcacct ggggccgaac gtggtggaga tggaaggccg gggcatagac gtggccctga
     5581 acatcgacac cgccaacgcc cagatcatcg acttgcagga ataccgggag gctatagacc
     5641 atgagcgcaa tcgacagagt gaagaaatcc agaggaatca acgagttagc ggagcagatc
     5701 gagccgctgg cccagagcat ggcgacactg gccgacgagg cccggcaggt catgagccag
     5761 acccagcagg ccagcgaggc gcaggcagcg gagtggctga aagcccagcg cgagacacag
     5821 gcagcatggg ccaagctggc caaggaattg cgggaagtgg ccagcgaggt gagcgacgcc
     5881 gcgcagagcg cccggagcgc ggccagaggg tggcactgga agctgtggct aaccgtgatg
     5941 gcggcttcca tgatgcctac gctggtgctg ctgatcgcat cgttgctctt gctcgacctg
     6001 acgccactga caacagagga cggctcgatc tggctgcgct tggtggcccg atgaaaaacg
     6061 acagaacctt gcaggccata ggccgacagc tcaaggccat gggctgtgag cgcttcgata
     6121 tcggcgtcag ggatgccacc accggccaga tgatgaaccg ggaatggtca gcctccgaag
     6181 tgctccagaa cacgccatgg ctcaagcgga tgaatgccca gggcaatgac gtgtatatca
     6241 ggcccgccga gcaggaacgc catggtctgg tgctggtgga cgacctcagc gagctcgacc
     6301 tggaggacat gaaagccgag ggccgggaac cggcgctgat cgtggagacc agcccgaaga
     6361 actatcaggc atgggtcaag gtggccgacg ccgcaggcgg tgaactccgg gggcagattg
     6421 cccggacgct ggccagcgag tacgacgccg acccggccag cgccgacagc cgccactatg
     6481 gccgcttggc gggtttcacc aaccgcaagg acaagcacac cacccgcacc ggctatcagc
     6541 cgtgggtgct gctgcgtgag tccaagggca agaccgccac cgctggcccg gcgctggtgc
     6601 agcaggctgg ccagcagatc gagcaggccc agcggcagca ggagaaggcc cgcaggctgg
     6661 ccagcctcga actgcccgag cggcagctta gccgccaccg gcgcacggcg ctggacgagt
     6721 accgcagcga gatggccgga ctggtcaagc gctacggtga cgacctcagc aagtgcgact
     6781 ttatcgccgc gcagaaactg gccagccggg gccgcagtgc cgaggaaatc ggcaaggcca
     6841 tggccgaggc cagcccggcg ctggcagagc gcaagcccgg ccacgaagcg gattacatcg
     6901 agcgcaccgt cagcaaggtt atgggtctgc ccagcgtcca gcttgcgcgg gccgagctgg
     6961 cacaggcacc ggcaccccgc cagcgaggca tggacagggg cgggccagat ttcagcatgt
     7021 aacgcttgca ttagcgctaa cgggtagtat actgttagct ctaatggagg atttgagatg
     7081 aaagaccatc gggacacgca gaccggcaac ctgttggcca cgcccaacgc gatcaggcag
     7141 gctaggtttg ccgacgctca gcgaaaactg ggccgcaagg cccgtaagat atgggccaca
     7201 gacgacgagg ccgaggcgct gcgtctgtac ctggaagaac tcagagcggc gcagggcggg
     7261 ggtagtgacc ccgccagcgc ctaaccacca actgcctgaa caggaggcaa tcatggctac
     7321 ccataagcct atcaatattc tggaggcgtt cacagcagcg ccgccaccgc tggactacgt
     7381 tttgcccaac atggtggccg gtacggtcgg ggcgctggtg tcgcccggtg gtgccggtaa
     7441 atccatgctg gccctgcaac tggccgcaca gattgcaggc gggccggatc tgctggaggt
     7501 gggcgaactg cccaccggcc cggtgctcta cctgcccgcc gaagatccac ccaccgccat
     7561 ccatcaccgg cttcacgccc tgggcgcata cctcaacgac gagcagcggc aagccgtggc
     7621 cgctggcctg ctgatccagc cgctgatcgg cagcctgccc aacatcatgg ccccggagtg
     7681 gttcgacggc ctcaagcgcg ccgccgaggg ccgccgcctg atggtgctgg acacgctgcg
     7741 ccggttccac atcgaggaag aaaacgccag cggccccatg gcccaggtca tcggtcgcat
     7801 ggaggccatc gccgccgata ccgggtgctc tatcgtgttc ctgcaccatg ccagcaaggg
     7861 cgcggccatg atgggcgcag gcgaccagca gcaggccagc cggggcagct cggtactggt
     7921 cgataacatc cgctggcagt cctacctgtc gagcatgacc agcgccgagg ccgaggaatg
     7981 gggtgtggac gacgaccagc gccggttctt cgtccgcttc ggtgtgagca aggccaacta
     8041 tggcgcaccg ttcgctgatc ggtggttcag gcggcatgac ggcggggtgc tcaagcccgc
     8101 cgtgctggag aggcagcgca agagcaaggg ggtgcccc
//
