LOCUS       NZ_AP025241             7659 bp    DNA     circular CON 28-MAR-2024
DEFINITION  Phocaeicola vulgatus strain MG01-10 plasmid pMG01-10_1, complete
            sequence.
ACCESSION   NZ_AP025241
VERSION     NZ_AP025241.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00405045
            Assembly: GCF_020885855.1
KEYWORDS    RefSeq.
SOURCE      Phocaeicola vulgatus
  ORGANISM  Phocaeicola vulgatus
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Phocaeicola.
REFERENCE   1
  AUTHORS   Tanaka,K., Noguchi,H., Vu,H., Muto,Y., Hayashi,M. and Yamamoto,Y.
  TITLE     Complete genome sequences of three Phocaeicola vulgatus isolated
            from a healthy Japanese individual
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7659)
  AUTHORS   Tanaka,K., Noguchi,H., Vu,H., Muto,Y., Hayashi,M. and Yamamoto,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-OCT-2021) Contact:Kaori Tanaka Gifu University;
            Yanagido, Gifu 501-1194, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Illumina Miseq; Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_020885855.1-RS_2024_03_28
            Annotation Date                   :: 03/28/2024 07:11:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,157
            CDSs (total)                      :: 4,047
            Genes (coding)                    :: 3,927
            CDSs (with protein)               :: 3,927
            Genes (RNA)                       :: 110
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 45 of 120
            Pseudo Genes (incomplete)         :: 87 of 120
            Pseudo Genes (internal stop)      :: 28 of 120
            Pseudo Genes (multiple problems)  :: 30 of 120
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP025241.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7659
                     /organism="Phocaeicola vulgatus"
                     /mol_type="genomic DNA"
                     /strain="MG01-10"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:821"
                     /plasmid="pMG01-10_1"
                     /geo_loc_name="Japan"
                     /lat_lon="35.4234 N 136.7606 E"
                     /collection_date="2020-09-20"
     gene            complement(join(7505..7659,1..295))
                     /locus_tag="GAIMETA21_RS20595"
                     /db_xref="GeneID:69842466"
     CDS             complement(join(7505..7659,1..295))
                     /locus_tag="GAIMETA21_RS20595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008656891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3791 domain-containing protein"
                     /protein_id="WP_008656891.1"
                     /db_xref="GeneID:69842466"
                     /translation="METNQTYQNELGSAMLPFVMRELVDTVMKRKTLPLEDALYYIYSS
                     NLYKALLDENTKLWYSSTLSLYEALEKEKTEQKRVQKDNPKILLFQMFCAENYRETKNI
                     SAKETLLLFSNHGVFEFLYENFEMLHTQDTEYILDTIITYINKKA"
                     /name="DUF3791 domain-containing protein"
     gene            complement(343..603)
                     /locus_tag="GAIMETA21_RS20600"
                     /old_locus_tag="GAIMETA21S10_39750"
                     /db_xref="GeneID:69842467"
     CDS             complement(343..603)
                     /locus_tag="GAIMETA21_RS20600"
                     /old_locus_tag="GAIMETA21S10_39750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005647331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_229509204.1"
                     /db_xref="GeneID:69842467"
                     /translation="MNKEMSLDVALDIIGTLRMMKIDEISEEKDENRKKILKKELSVLN
                     TEEKIANGLLQFEVSENVRLSVMDKIQNYYAPKLKAYYAAL"
                     /name="hypothetical protein"
     gene            complement(600..1058)
                     /locus_tag="GAIMETA21_RS20605"
                     /old_locus_tag="GAIMETA21S10_39760"
                     /db_xref="GeneID:69842468"
     CDS             complement(600..1058)
                     /locus_tag="GAIMETA21_RS20605"
                     /old_locus_tag="GAIMETA21S10_39760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_229509206.1"
                     /db_xref="GeneID:69842468"
                     /translation="MSDIQTSTIRVPKNVLEDIKIYCRKAGQPVGEWVEKAWNFLQKND
                     FDIYDTEVTPFLPVPAEVERERNQVDALCKLMSEFIISQKQAQLPTPDIIAKATEEKVR
                     ADFLEKELQQLREENKALRERYEKAHKELVRVQIEQKTLGKIKVNTDL"
                     /name="hypothetical protein"
     gene            complement(1137..1304)
                     /locus_tag="GAIMETA21_RS20610"
                     /old_locus_tag="GAIMETA21S10_39770"
                     /db_xref="GeneID:69842469"
     CDS             complement(1137..1304)
                     /locus_tag="GAIMETA21_RS20610"
                     /old_locus_tag="GAIMETA21S10_39770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005799222.1"
                     /db_xref="GeneID:69842469"
                     /translation="MEELKVKVVEVSQEKEQPQGNDYIRVTVEIDGRKIAVWTTESNIN
                     ALRFDIDKKR"
                     /name="hypothetical protein"
     gene            complement(1307..2281)
                     /locus_tag="GAIMETA21_RS20615"
                     /old_locus_tag="GAIMETA21S10_39780"
                     /db_xref="GeneID:69842470"
     CDS             complement(1307..2281)
                     /locus_tag="GAIMETA21_RS20615"
                     /old_locus_tag="GAIMETA21S10_39780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799221.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_229509207.1"
                     /db_xref="GeneID:69842470"
                     /translation="MNINNKDVIQSYIMTTAKYDFSADEKRILLRLVETWQYLLEGKEL
                     KGKIDRDLFGGYILEFPISYFVPDNQTNYKRIKDALRSLNDKKFEYEDSDLWEIIRIIE
                     RPQIRKREKVIFELNEKIVECFLNFTKGYRKFELETALSFSSVYAVRFYELMSGQTTPL
                     TFTFTKLKEMFQLTDKYPRTPDFIKRVIEPAKRELDEKSPFSFEYRYKEPDMKDGKKFY
                     KIMFYPIAIPRNRDEHVERKDLLSQKVNLSAFLDKVDREYLAKLGFSDRQIKNNYDLIL
                     SAKKELDLVYELSLLIGKVRGKKNPQGYVISTLKGKLKDKKIK"
                     /name="replication initiation protein"
     gene            3203..3712
                     /locus_tag="GAIMETA21_RS20620"
                     /old_locus_tag="GAIMETA21S10_39790"
                     /db_xref="GeneID:69842471"
     CDS             3203..3712
                     /locus_tag="GAIMETA21_RS20620"
                     /old_locus_tag="GAIMETA21S10_39790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008656880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BfmA/BtgA family mobilization protein"
                     /protein_id="WP_141982369.1"
                     /db_xref="GeneID:69842471"
                     /translation="MEEKRPNTPRNSTVAVSPEIGKKLERFCASCGITKKDFISLSLDY
                     FQRYGINPAKHESPAKEMEKLIKKNDQVIGFIRKQEQDILRPMLEAITTTEERIKANIG
                     SLATSDKLIEFIQAVDSNSNIQVDTRDKLAETISKQNKEVKQAILTLARYLDEKGKAGL
                     IDRLFG"
                     /name="BfmA/BtgA family mobilization protein"
     gene            3712..4500
                     /locus_tag="GAIMETA21_RS20625"
                     /old_locus_tag="GAIMETA21S10_39800"
                     /db_xref="GeneID:69842472"
     CDS             3712..4500
                     /locus_tag="GAIMETA21_RS20625"
                     /old_locus_tag="GAIMETA21S10_39800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5712 family protein"
                     /protein_id="WP_229509209.1"
                     /db_xref="GeneID:69842472"
                     /translation="MIVKVQGGGKVYANTGSCSDVVSYLQHEDAERLQAGLTPEPFFNQ
                     EQDRISPKEVTNKIDNNRKGLKAKEAKFYVITVAPSRAELREMGATKEEQAAAMKEYIR
                     TEVMPKYAEGFNKGLKADDLEFYGKIHFERHEKDGEDLHAHIIVSHKTKNNGKSISPMT
                     NHTGKKNTGAAQGGFNRKEWYSSCEKAFDKHFKNERDIKESFEYKNAMKNGTPKEMQEQ
                     INRAIQQERQREQQIRQQQEQRVTQAVKTEKKENKVKPKL"
                     /name="DUF5712 family protein"
     gene            4505..6169
                     /locus_tag="GAIMETA21_RS20630"
                     /old_locus_tag="GAIMETA21S10_39810"
                     /db_xref="GeneID:69842473"
     CDS             4505..6169
                     /locus_tag="GAIMETA21_RS20630"
                     /old_locus_tag="GAIMETA21S10_39810"
                     /inference="COORDINATES: protein motif:HMM:NF014581.4"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA transfer
                     family protein"
                     /protein_id="WP_141982373.1"
                     /db_xref="GeneID:69842473"
                     /translation="MKEGQLKSSNRSGCLMYLALIYAFPMLAALAVLPLGVVYLCFRAG
                     EIGNGLFVLAVEAGAVYLFIKYKRKGKKEEAPKNPNTAVLRTEAGGVQLGNPFRGVFVL
                     GAAGSGKSESIAVPLLSEFVRMGFAGVVYDFKFPTLAKDVQSFVNAKGSVLRHFYLDFN
                     NPAASYRVNPLNPRYLPNTSYAREYAQAIISNLMKESIKKPDFWTRSATDLLTACIWYL
                     KEERPDICDLPHVLAMITSNDTALLKTLQKNVQTAQMTVSIYNAMERGADSQVSGVIGT
                     LQGAIAQINTPELMFLFSGDDFSLDVNDPQNPIILTVGSYPTLTQTFAPLCSLVITVAT
                     KLMNQPDKHPSFVLLDEAPTVFIPNIEVLPNTGRSNKIATVIMCQDIAQLEDGYGREKA
                     DVLFASCNSHFYGRVASSKTAEILSKQFGKADKVYITSNEGRNTRKFGKTLGRSETIQE
                     RDVIKPSEFLNLQVGEFAGLAVESNKPTFRTRFKQAQRPQPAELKKPDFSGSVSDYYKR
                     VRYDINKMLEIGGTAGEYDRDTMKLNETKRNFFGVEY"
                     /name="type IV secretory system conjugative DNA transfer
                     family protein"
     gene            complement(6298..6717)
                     /locus_tag="GAIMETA21_RS20635"
                     /old_locus_tag="GAIMETA21S10_39820"
                     /db_xref="GeneID:69842474"
     CDS             complement(6298..6717)
                     /locus_tag="GAIMETA21_RS20635"
                     /old_locus_tag="GAIMETA21S10_39820"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_141982375.1"
                     /db_xref="GeneID:69842474"
                     /translation="MKIKELFIIFGMLNTLLLFSCISKPNKEDSALTDKDIAQFDSLFY
                     AWEKEISSNPKTKYNSSTQTYTTLPQFKELVSMGKKIIPCIIERFEKDSNSFFAIPLYN
                     ELQEIKELKSCTKTSEQEKMREIISKFREQEKAKK"
                     /name="hypothetical protein"
     gene            complement(6747..7007)
                     /locus_tag="GAIMETA21_RS20640"
                     /old_locus_tag="GAIMETA21S10_39830"
                     /db_xref="GeneID:69842475"
     CDS             complement(6747..7007)
                     /locus_tag="GAIMETA21_RS20640"
                     /old_locus_tag="GAIMETA21S10_39830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005647331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_229509202.1"
                     /db_xref="GeneID:69842475"
                     /translation="MNKEMSLDVALDIIGTLRMMKIDELSKETDPIKIEILEKEFNVLT
                     IEERIAQGLEQFEGWKDVRLSVMDKIQNYYAPKLKAYYATL"
                     /name="hypothetical protein"
     gene            complement(7029..7508)
                     /locus_tag="GAIMETA21_RS20645"
                     /old_locus_tag="GAIMETA21S10_39840"
                     /db_xref="GeneID:69842476"
     CDS             complement(7029..7508)
                     /locus_tag="GAIMETA21_RS20645"
                     /old_locus_tag="GAIMETA21S10_39840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005647329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
                     /protein_id="WP_032844865.1"
                     /db_xref="GeneID:69842476"
                     /translation="MKLYHGSTVTVKSPNIQKGRKATDFGKGFYTTTNFEQAKKWAILK
                     RNREHGKKAVVSVYEVPDNILDREFSVLRFEGATKEWLEFVVNNRRGKGKNSYDLIMGP
                     VANDQLYATIRLYEQGVVTADAAIEMLKTHKLFNQLSFHTVKVIPLLKFTESIEV"
                     /name="DUF3990 domain-containing protein"
     Typing          3713..4368
                     /name="MOBB"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.543"
                     /coverage="84.0"
                     /evalue="5.07e-146"
ORIGIN
        1 tataattttc agcacaaaac atttgaaaaa gcaatatctt tggattgtcc ttttgtactc
       61 tcttctgttc tgttttctct ttttctaatg cttcataaag tgataatgta cttgaatacc
      121 acaattttgt attctcgtcc aataatgcct tgtacaaatt ggaagaatat atatagtata
      181 acgcatcttc taaaggtagc gtttttcttt tcatgactgt atcaaccaat tctctcataa
      241 caaatgggag catagccgaa ccaagttcat tttgatatgt ttgattagtt tccataacta
      301 ttgaatttat catgtttgaa aagttggttt tttgtcccta tctcatagtg ctgcatagta
      361 tgctttcaat ttgggtgcgt aatagttttg tatcttatcc atgactgata agcgcacatt
      421 ttcagaaact tcaaactgca acaatccatt tgctattttt tcttctgtgt tgagaacaga
      481 aagttctttt ttcagtattt tttttctgtt ttcatccttc tcttctgata tttcatctat
      541 tttcatcatg cggagtgtcc cgataatgtc gagcgcaaca tccaagctca tttctttatt
      601 cataaatctg tatttacttt tattttccct aaagtttttt gctcaatctg aacccttaca
      661 agttctttat gtgctttttc gtaccgttca cgcagagcct tgttttcttc ccgaagctgt
      721 tgcaactcct tttctaaaaa atcggctctt actttttctt cggtagcttt ggcgatgata
      781 tccggtgttg gtagttgtgc ctgtttttgc gagattataa attcgctcat cagcttacaa
      841 agtgcatcca cttggttacg ctctctttct acttcagccg gaacaggcag aaagggggtt
      901 acttcagtat cgtaaatatc gaaatcgttc ttttgcaaga aattccatgc cttttccacc
      961 cattccccga caggttgtcc cgctttacgg caatatattt ttatatcttc aagcacgttt
     1021 ttaggtactc taatggtgct tgtttgtata tcgctcatgt atctatttgt taattagtta
     1081 tatgcaaaaa tataaagaat atatttagct ttctacaaaa tctacaaaaa atatatttat
     1141 ctctttttgt caatatcaaa gcgtagagca ttgatgttgc tttcggtagt ccatacggct
     1201 atttttcgcc cgtcaatttc aacggtaaca cgaatataat cgtttccttg tggttgttcc
     1261 ttttcctgtg aaacttccac tactttaact tttaattctt ccatgtctat tttatttttt
     1321 tgtcctttag ttttcctttt agggtactta ttacatatcc ttgtgggttc ttctttcctc
     1381 taactttccc gataagtaag gacagttcat acacaaggtc taactccttt ttagctgaca
     1441 atattaagtc atagttgttt tttatttgcc tatcagagaa ccctaacttt gcaaggtatt
     1501 ccctatccac cttatccaag aacgcagata aattaacttt ttgggataat aggtcttttc
     1561 gttctacatg ttcatccctg ttgcgtggaa ttgcaatagg atagaacatg attttataga
     1621 atttctttcc gtctttcatg tccggttctt tgtatctgta ttcaaatgaa aatgggcttt
     1681 tttcgtctaa ttcccttttt gcaggttcaa tgacacgttt tatgaaatcg ggtgtacgtg
     1741 ggtatttgtc tgtaagttga aacatttctt tcagcttagt gaaagtaaag gttaaaggtg
     1801 ttgtttgccc actcattagc tcatagaagc gtacagcata gacagatgaa aaggatagag
     1861 ccgtttctaa ctcaaacttt ctgtaacctt tggtaaagtt aaggaagcac tctacaatct
     1921 tctcgtttag ctcaaaaata accttttctc gtttccgaat ttgcggtctt tctataatgc
     1981 gtataatttc ccataggtct gaatcttcat attcaaattt cttatcattg agagaacgca
     2041 aagcgtcttt tatacgtttg tagttcgttt gattatctgg aacaaagtag cttataggaa
     2101 actctaggat atagccgcca aataaatctc tatctatttt tccctttagt tctttgccct
     2161 ctaacaagta ttgccatgtt tcaaccaagc gcaagagaat gcgcttttca tccgcagaaa
     2221 agtcatattt tgcggtagtc ataatatacg actgaataac gtctttattg tttatattca
     2281 tttaattacg ttttaaaaac gacaacttgc atagttgttt cgcagcaaat atacatataa
     2341 aaacgacata cagaaaacaa agtctatttt attttagaaa actatgcaaa aagtcgtctt
     2401 tgaaaccctt aaaaactatg caaaaagtcg tctttaacta tgcaaaaagt cgtcttttga
     2461 aaaataaaat gaatataatc gattgattta taataagata taacgtgctt ttaaaaagtg
     2521 ataagcatag acaagttatg ctatttaaca caaagtattg attttagaat ataagtagtt
     2581 gaatatcaaa acaatagggg aaacaggacg actttttgca taatggaaaa gcaaagcaag
     2641 aagccaagcg cagcggggaa aaggaagaaa tttccccgcg tgttaaaaaa tgtatattta
     2701 tgggtaattt tgtatttttt tgattttggt agtttaggca cttcttgcta taaccatttg
     2761 ttatacagac tgtttgcaat actcttccaa ttctttacaa atgtattctt tcaatgttat
     2821 cccctctttc tttgcttgtc tttcaaagat acatattccc catttactta gctgtttgtg
     2881 tctgttaaat tatgcgcagt cggaactatt cctattagcc cgccatacat ccgactgcat
     2941 aacccccgcc cccttgtgca ttggcgcagc tcacgcccgc ccgcaccgca ggggaggggt
     3001 attccgtcgg ctgcttaggc agtcgaatag gaatagcaag aggaaacgcc acagagttgc
     3061 gttttttcct cttgccctac gggattacgg ctgaaaatca gccgtttata aaaatcgaat
     3121 gattttttat atatagtatt gatatattgt aataaataac tacttttgta tcagtactta
     3181 atgatatata aaccgttata atatggaaga aaaaagacct aatactcccc gcaattctac
     3241 tgttgccgtt tctcccgaaa tcggtaagaa attagagcgt ttttgtgctt catgtggtat
     3301 cacgaagaaa gattttattt ccctttcgtt agactatttt caacggtacg ggataaatcc
     3361 ggctaagcat gaaagccccg ccaaagaaat ggaaaaacta atcaagaaga acgaccaagt
     3421 aatcgggttt atccgtaagc aggagcagga cattttgcgc cccatgcttg aagccataac
     3481 aaccaccgaa gaaagaataa aggctaatat cgggagtttg gctacttctg ataagttaat
     3541 agaatttata caagccgtag acagcaattc caatatacag gtagatacga gagataaatt
     3601 agcagaaaca atctccaaac agaacaagga agtaaaacaa gcgattttaa ctcttgcacg
     3661 ctatttggac gaaaagggaa aagcgggttt aattgataga ctttttgggt gatgatagta
     3721 aaagtacagg gaggcggtaa agtttacgcc aacactggca gctgctctga tgttgtttcc
     3781 tatttgcagc atgaagatgc agaacggctg caagcagggc taacgcctga accgttcttt
     3841 aatcaggaac aggacaggat aagccccaaa gaagtaacca acaagataga caacaaccga
     3901 aagggcttga aagccaaaga agcaaagttc tatgttatca ctgttgcgcc gtcacgtgcg
     3961 gaactccgtg aaatgggagc cacgaaagag gaacaggcag cagccatgaa agagtatatc
     4021 cgtaccgaag tcatgccgaa atatgcggag gggtttaata aagggctgaa agctgatgat
     4081 ttggagtttt acggtaaaat ccattttgaa cggcacgaaa aagacggaga agatttgcat
     4141 gcccatatta tcgtaagcca taagaccaag aataacggta aatcaatcag ccccatgaca
     4201 aaccacacag ggaaaaagaa tacaggtgca gcacaagggg gatttaaccg caaagagtgg
     4261 tattcttcat gtgaaaaggc ttttgataaa cattttaaaa atgaacgtga tataaaggaa
     4321 agttttgaat acaagaacgc catgaagaac ggcacaccaa aagaaatgca ggaacagata
     4381 aacagagcta ttcagcagga acggcagagg gagcaacaga tacgacagca acaggaacag
     4441 agggtaacac aagctgtcaa gacagaaaaa aaggaaaata aagtaaaacc aaaattataa
     4501 aatcatgaaa gagggacaat taaaaagtag caaccgtagc ggttgtttga tgtatttggc
     4561 attgatttac gctttcccca tgcttgcagc tttggcggtt ttgcctttgg gtgtggtgta
     4621 tttgtgtttt cgtgccggag aaatcggaaa cggtttgttt gtgcttgcgg tggaagctgg
     4681 agcagtctat ttgtttatca aatacaaacg taagggaaaa aaagaggaag cccccaagaa
     4741 cccgaatacg gcagttttgc ggacagaagc cggaggggtg cagttgggaa acccatttag
     4801 aggggttttt gtattaggag cagccggaag cgggaaaagt gaaagtattg cagtgccttt
     4861 gctttccgag tttgtccgca tggggtttgc gggtgtggta tatgatttca aatttccgac
     4921 attggcaaaa gatgtacaat cctttgtaaa cgccaaagga agcgttttaa gacactttta
     4981 tttggatttc aataatccgg ctgcatctta cagggtaaac cctttaaatc cacgatattt
     5041 gcctaatacg tcttatgcac gtgagtacgc acaggcaata ataagcaatc taatgaaaga
     5101 gagtatcaag aagccggact tttggacgag aagcgcaacc gacctcttga ccgcctgtat
     5161 ttggtatctg aaagaggaac gccccgatat ttgcgattta ccgcacgttt tagccatgat
     5221 aacgagtaat gataccgcac tacttaaaac tttgcagaag aacgtacaaa cggcacaaat
     5281 gaccgttagt atttacaatg caatggagag gggggcagac agccaagtaa gcggggttat
     5341 tggcacatta caaggagcaa tagcacagat aaacactccc gaacttatgt tcttgttttc
     5401 gggtgatgat ttctcgctcg atgtgaacga cccgcaaaac cctattattt tgacagtcgg
     5461 aagctacccg acattaacac agacttttgc cccattgtgc tctttagtga taacggttgc
     5521 taccaagctg atgaaccagc cggataaaca tccgtctttt gtccttttgg atgaagcacc
     5581 gaccgtattt ataccgaata tcgaagtttt gccgaataca ggcaggagca acaagatagc
     5641 aacggttata atgtgccagg atattgcaca gttagaggac ggttacggta gggaaaaagc
     5701 cgatgttttg tttgcttcct gcaattccca tttctatggc agggtagcaa gcagcaaaac
     5761 ggctgaaatc ctttctaaac aattcggaaa agccgataag gtatatataa catccaacga
     5821 ggggcgcaat acaaggaagt ttggaaaaac tttggggagg tctgaaacaa tacaggaacg
     5881 tgatgttatc aagccgtcag aatttttaaa cttgcaagtt ggagagtttg cagggttggc
     5941 agttgaaagc aataagccca cattcagaac ccgatttaaa caggcacaaa gaccgcagcc
     6001 cgcagaattg aagaagcccg acttttccgg ctctgtgagc gattattaca agcgggtgag
     6061 atatgatata aataaaatgc ttgaaattgg aggaactgcc ggagagtacg acagggacac
     6121 tatgaagctg aacgaaacaa agagaaattt ctttggggtt gagtattgaa aacctttatc
     6181 attttgatag tcgctttggt ctgaatgggg gaagaaccgc cccccccatc agccaagtac
     6241 tgaataccct tttgtaacac ccgacaatac tatttttgtg gtttatgtgc gatattacta
     6301 tttttttgct ttttcttgtt ctctaaattt actaataatt tctctcatct tttcttgctc
     6361 gctcgtttta gtacatgatt taagttcttt aatttcttgt aattcgttgt ataaaggtat
     6421 tgcaaagaat gaattactat ctttttcaaa gcgttctatg atacaaggaa tgattttttt
     6481 ccccattgat acaagctctt tgaattgtgg taatgttgtg tatgtttgag tacttgaatt
     6541 atattttgtt tttggattag aggaaatttc tttttcccac gcataaaata gactatcaaa
     6601 ttgggcaata tctttatctg ttaaagcact atcttcctta tttggtttac tgatacaact
     6661 aaataaaagc agtgtgttaa gcattccaaa tataataaac agttctttta ttttcatgtt
     6721 gttttaaatt taaaagtttc tctacttcat agcgttgcat agtatgcttt caatttgggt
     6781 gcataatagt tttgtatctt atccatgact gataagcgca cgtctttcca gccttcaaat
     6841 tgttcaagtc cttgcgctat tcgttcttct atggtaagta cattaaattc tttctccaat
     6901 atttctattt ttattgggtc tgtttctttt gataattcat ctattttcat catgcggagc
     6961 gtcccgataa tatcaagggc aacatccaag ctcatttctt tattcatgat tcattatagt
     7021 tttatggttt atacttcaat agattctgtg aattttaata atgggataac tttcactgta
     7081 tgaaatgaaa gttggttaaa aagcttatgg gtctttagca tttcaattgc tgcatcagcc
     7141 gtaacaacac cttgctcata aagccggatt gttgcatata actggtcatt ggcaacgggt
     7201 cccattatta aatcatagct gttttttcct tttcctctgc ggttattaac tacaaactcc
     7261 agccactctt ttgtagcacc ctcaaaccga aggactgaaa actctctatc aagaatatta
     7321 tcggggactt cataaactga aacgacagct tttttaccat gttcccgatt ccttttgagt
     7381 attgcccact tcttagcctg ttcaaaatta gttgtcgtat aaaatccttt tccaaagtca
     7441 gtagctttac gacctttctg tatattaggg gatttgacgg taactgttga gccgtgatat
     7501 agtttcatgc ctttttattg atataagtta ttatagtatc taatatatac tctgtatctt
     7561 gtgtgtgcag catttcaaaa ttttcatata gaaattcaaa aacgccatga ttggaaaaca
     7621 gcaaaagagt ttctttagca cttatatttt tagtttccc
//
