LOCUS       NZ_AP025219             1465 bp    DNA     circular CON 18-FEB-2024
DEFINITION  Escherichia coli strain 2017.09.02CC plasmid p20170902CC-5, complete
            sequence.
ACCESSION   NZ_AP025219
VERSION     NZ_AP025219.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00407182
            Assembly: GCF_020886495.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Yamamoto,Y., Higashi,A., Ikawa,K., Yamaguchi,T., Kawahara,R.,
            Nguyen,T.N., Khong,D.T. and Tran,H.T.
  TITLE     Horizontal transfer of mcr-1 marked with a single nucleotide
            mutation between clinical isolates from community residents
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1465)
  AUTHORS   Yamaguchi,T. and Kawahara,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2021) Contact:Takahiro Yamaguchi Osaka Institute
            of Public Health, Division of Microbiology; Higasinariku nakamichi
            1-3-69, Osaka, Osaka 537-0025, Japan URL :https://www.iph.osaka.jp/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 72x
            Sequencing Technology :: MGI DNBSEQ-G400RS; ONT MinION Mk1c
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/18/2024 04:27:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,854
            CDSs (total)                      :: 4,729
            Genes (coding)                    :: 4,576
            CDSs (with protein)               :: 4,576
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 58 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 35 of 153
            Pseudo Genes (multiple problems)  :: 35 of 153
            Pseudo Genes (short protein)      :: 3 of 153
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP025219.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="2017.09.02CC"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p20170902CC-5"
                     /geo_loc_name="Viet Nam:Thai Binh"
                     /collection_date="2017-11"
     gene            complement(join(710..1465,1..114))
                     /locus_tag="LPH32_RS24170"
     CDS             complement(join(710..1465,1..114))
                     /locus_tag="LPH32_RS24170"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phasyl DNA replicon protein arp"
                     /protein_id="WP_174243766.1"
                     /translation="MLLKKDRFGKLTSETLNAIAQTGVREAIADLPCLYRNNSIEITDK
                     TPSKPLSPAAFELSTNLKKSASALAWNVQHFVDTYGLSNVGFLTLTFRDHVTDPKEAQR
                     RFNSLKSNVLASRYRAYIRVMEPMKSGRIHYHLLVALDSDIRTGFDFVAVKNQDYSTAN
                     KAIRAEWSFWRKTAPKYRFGRTELMPVRSNSEGIGRYVGKYISKGIESRTEQFKGTRLV
                     EYSRKAKIASTRFQFVTEGSAEWRRKLAIFVHYIADNTGCEPSFEGLRRVLGARWSYHW
                     REFILSLT"
                     /name="phasyl DNA replicon protein arp"
ORIGIN
        1 gttgtttcta tatagacaag gaaggtcggc tatcgcctcc ctgacgccag tttgagcaat
       61 ggcgttaaga gtttcagaag tgagtttgcc gaagcgatct tttttaagta gcattagtcc
      121 atcacctaga gtctgagtgt cgaaaacgaa tttctgtagg tggtcatgcc cccgaagagt
      181 ttgccgctct tgcgggggtt tccatttcat cgtatgagta aggtgcggtg cgcaactccc
      241 gctaaaaatt atttataatc aatacacgac cgccaaaaag aataccgcaa aaattcacct
      301 aaatcatcaa tcactgtctt accagtgttt ttctcgcaaa atctgattta cggatacctc
      361 ctcgcaccgc tgcgggggta tccgtaaatc agccttcgct ccaaaaaaat gaccaggctt
      421 tgttcgctgt tttacatgaa tacatgagtg catgagtaat cgcagaatga gacgaaagtg
      481 cagtattaca ttcactgcac ttgaaaagcg gtttaacagg gcgatggctc accatgtgat
      541 ccatcaccta tcgaaagtgt cggggggggg agtgttgtac aatcactaaa tcggaaccct
      601 aaagggatcc cccgatttag aaattgacaa ttcagcaacg gtgagggctg tcagatttta
      661 atctggcagc gtgaggcgga attgcagatt tgagaaaaaa gtacaggtgt taggtgagtg
      721 ataaaataaa ttcgcgccag tgataagacc accgcgcacc taaaacacgg cgaagaccct
      781 caaaagatgg ctcgcaaccc gtattgtcag cgatgtaatg aacgaagata gctaacttac
      841 gacgccactc agcagaaccc tctgtaacga actggaagcg cgtagaagcg attttagctt
      901 tacgggaata ctcaaccagc cgagtccctt taaattgctc tgtacgcgat tctatgccct
      961 tagaaatgta tttcccaacg taacgcccaa taccctctga atttgaacga acgggcatca
     1021 gttcggtacg cccaaatcgg tatttggggg cagttttgcg ccagaatgac cactcggcac
     1081 ggatcgcctt gttagcggtt gagtaatcct gatttttgac ggctacgaaa tcaaagccgg
     1141 ttcggatgtc ggaatcgaga gcaacaagca aatgataatg gatgcgaccg gacttcatag
     1201 gctccatgac gcgaatataa gcccggtaac gcgaggccaa aacattgctt ttaagagagt
     1261 taaaacggcg ttgtgcttct ttgggatcgg taacgtggtc acgaaatgtc agggttaaaa
     1321 aaccaacgtt tgaaaggccg taggtgtcaa cgaaatgctg tacattccac gccagagccg
     1381 aggcagattt tttgaggttt gtagaaagct caaaagccgc aggtgacaag ggtttggaag
     1441 gtgttttgtc ggtaatctcg attga
//
