LOCUS       NZ_AP024953             5510 bp    DNA     circular CON 30-JAN-2024
DEFINITION  Aeromonas caviae strain KAM345 plasmid pKAM345_5, complete sequence.
ACCESSION   NZ_AP024953
VERSION     NZ_AP024953.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00294561
            Assembly: GCF_019972775.1
KEYWORDS    RefSeq.
SOURCE      Aeromonas caviae
  ORGANISM  Aeromonas caviae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Aeromonadales;
            Aeromonadaceae; Aeromonas.
REFERENCE   1
  AUTHORS   Maehana,S., Suzuki,M. and Kitasato,H.
  TITLE     Draft genome sequence of carbapenem-resistant Aeromonas spp. in
            Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5510)
  AUTHORS   Maehana,S., Suzuki,M. and Kitasato,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUL-2021) Contact:Shotaro Maehana Kitasato university,
            Allied Health Sciences; 1-15-1, Kitasato, Minami-ku, Sagamihara,
            Kanagawa 252-0373, Japan URL
            :https://www.kitasato-u.ac.jp/ahs/english/index.htmltmRNA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8.0
            Genome Coverage                   :: 193x
            Sequencing Technology             :: Illumina HiSeq X; ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/30/2024 10:21:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,616
            CDSs (total)                      :: 4,457
            Genes (coding)                    :: 4,339
            CDSs (with protein)               :: 4,339
            Genes (RNA)                       :: 159
            rRNAs                             :: 11, 10, 10 (5S, 16S, 23S)
            complete rRNAs                    :: 11, 10, 10 (5S, 16S, 23S)
            tRNAs                             :: 122
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 30 of 118
            Pseudo Genes (incomplete)         :: 89 of 118
            Pseudo Genes (internal stop)      :: 12 of 118
            Pseudo Genes (multiple problems)  :: 10 of 118
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP024953.1.
            Annotated at DFAST https://dfast.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5510
                     /organism="Aeromonas caviae"
                     /mol_type="genomic DNA"
                     /strain="KAM345"
                     /db_xref="taxon:648"
                     /plasmid="pKAM345_5"
                     /geo_loc_name="Japan:Kanagawa"
                     /collection_date="2018"
     gene            159..1148
                     /locus_tag="LDN68_RS23045"
                     /old_locus_tag="KAM345_044710"
     CDS             159..1148
                     /locus_tag="LDN68_RS23045"
                     /old_locus_tag="KAM345_044710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005321767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_113740805.1"
                     /translation="MVKPATAAFVERLPSRPYCTNDKSAGLLIRPQATALAYRHIQHNP
                     PPHVSCLVFDVDKKPHELRRRDGYHEWRERGLPAPHWISINPENGNYHLGYLLAAPVAR
                     TNAARLKPLRYLAAIEHVLAHRLGADVGYVGLITKNPVHSDWWTVWHGIEPYSLDYLAE
                     FCPDADLAAYNRRSRKEASGLGRNVTLFDNVREWAYSAVRAHWRPNGYDAWAEAVHAAC
                     DSANAFGLEQGGPLPVSETKATAKSIARWVWRHFSPAEFRAIQAARGAKGGRVSKGGGR
                     PSNSGIARADLLPEVLRLKAQGYSNRDIAEDLQISAGSVSNYLRRDRE"
                     /name="replication initiation protein"
     gene            1379..1624
                     /locus_tag="LDN68_RS23050"
                     /old_locus_tag="KAM345_044720"
     CDS             1379..1624
                     /locus_tag="LDN68_RS23050"
                     /old_locus_tag="KAM345_044720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017412821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication protein RepB"
                     /protein_id="WP_113740795.1"
                     /translation="MQERDAKTVFDLGGFSKALIAPTPMGAGYHLHLVRFGKGGETEVI
                     ERQRGGWRVFTTIDAAANTANQIGFRRIEVDLSSRR"
                     /name="plasmid replication protein RepB"
     gene            complement(1629..3215)
                     /locus_tag="LDN68_RS23055"
                     /old_locus_tag="KAM345_044730"
     CDS             complement(1629..3215)
                     /locus_tag="LDN68_RS23055"
                     /old_locus_tag="KAM345_044730"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WP_139424258.1"
                     /translation="MVYPRKMDSQPSQLGTIPNACRQFFAPQGRVYVFEDEGLKRINIV
                     GRYVYMPPPTPAYLGGTLMTVFLMGVSMSGYQFAHLDSYARKASSKVAGGKSAQDIADE
                     AERKKGAYKHLDIEQVKDPIIQFGMTPSEVVKVATEWAEQAKDPKGKKLRIDGQCMAAG
                     VFSVPEDFPEDRWEKYREAMIEHLKGKHGKRLRSVVEHVDEPYRHCHFYLVPLPGESYG
                     VVHPGWAAEKASYARGERKGLQTKAYSEAMSAWQDEIHTISARFGLLRTGPRRARLPNK
                     DYKAAKRAASLIAEQVSTPPLSKPKLPPVEEIIDALEPLHSTGILGRGEPLYTRAQLLK
                     ASKKSALYGHKHAAQQQLDYLTQTTERAAEIDAAEARIKELHDKAEAQAAEIRAGEDKM
                     RALRDEERKQTELLGRLELAVKKTQGYVDEAISRVQERALKALGGVLDAFVAIVEAPQD
                     QELKDAAKSGLESIGISGAWAKLADRVVNVLMPSARAAQAADGIDWPKEVKETGRKLRD
                     RTVDSSSDMGM"
                     /name="mobilization protein"
     gene            3512..3946
                     /locus_tag="LDN68_RS23060"
                     /old_locus_tag="KAM345_044750"
     CDS             3512..3946
                     /locus_tag="LDN68_RS23060"
                     /old_locus_tag="KAM345_044750"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162628491.1"
                     /translation="MKLKCLFITSGAFLLSACTSISQQEAGSINYGDKPVNYDKAIISA
                     IKNNLKDPDSMKVVSITNPYRVYSKCGFGTVKYGWYTTIRYNAKNSYGGYVGEKEFNYA
                     YLNGKYDVGMMCSGGSWNGNGNSILLDEITYLCKGDCPQN"
                     /name="hypothetical protein"
     gene            4637..4897
                     /locus_tag="LDN68_RS23065"
                     /old_locus_tag="KAM345_044760"
     CDS             4637..4897
                     /locus_tag="LDN68_RS23065"
                     /old_locus_tag="KAM345_044760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005323888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_223918470.1"
                     /translation="MDTITHPTLVQLVAAGAVRVVVAVGQPGGWTLLVRYGLAERALAA
                     QRSKQLRVFRKLDTLQAYLQEIGVVRFEIDAAGFVVAPRQR"
                     /name="hypothetical protein"
     gene            4969..5211
                     /locus_tag="LDN68_RS23070"
                     /old_locus_tag="KAM345_044770"
     CDS             4969..5211
                     /locus_tag="LDN68_RS23070"
                     /old_locus_tag="KAM345_044770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017736327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="WP_113740798.1"
                     /translation="MGTINLRIDDDLKARSYAVLDRLGVTPSELLRQTLEYVAAQERLP
                     FRTTVVTQDEEALLALVRERLANPQKGIKVSLDDL"
                     /name="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
     gene            5201..5488
                     /locus_tag="LDN68_RS23075"
                     /old_locus_tag="KAM345_044780"
     CDS             5201..5488
                     /locus_tag="LDN68_RS23075"
                     /old_locus_tag="KAM345_044780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005321942.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_113740797.1"
                     /translation="MTFELEFDPRAWREWKKLGDTVRQQFKKKLVEVLERPRNEANRLH
                     GLPDCYKVKLRGAGYRLVYQVQDDRVVVFVVAVGKREREQVYLDAGSRLE"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     Typing          complement(1633..3215)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.811"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agtgctgtaa cttcttgtcc agaaaagaca aaccccgaat ctctgtattc aacttggcgg
       61 aagagtcaga gagttcgggg ttgtgacgaa tcacgaggta aagaatacca catggatcac
      121 atcaaacaac cctgcgccgc caataagcgc ggtgaccgtt ggtaaaacct gcaacagcag
      181 cattcgtcga gcggctcccc agtcgcccct actgcaccaa cgacaaatcg gctggactgc
      241 tcattcgtcc acaggccaca gcactggcct atcgccatat ccagcacaac ccaccgcccc
      301 atgtatcctg tctggtgttt gacgtggaca agaaaccgca tgagctgcgt cggcgagatg
      361 ggtatcacga atggcgggag cgtggtttgc ccgctccgca ttggatctcg atcaaccccg
      421 agaacgggaa ttaccatctt ggctatttgc tagccgcgcc cgtggcacgt accaatgcgg
      481 ctaggctaaa gccgttgcgc tatctggcgg ccatagaaca cgttttggcg catcggctgg
      541 gtgctgacgt tgggtatgtt ggccttatca ccaaaaaccc cgtacacagc gattggtgga
      601 cggtatggca cggcattgag ccctattccc tcgactacct cgcggagttc tgcccggatg
      661 ccgacttggc ggcctacaac cgccgcagcc gcaaggaggc gagcgggctg gggcgtaacg
      721 tcaccctgtt cgataacgtt cgggaatggg cctacagcgc cgtcagagcg cactggcgac
      781 ccaatgggta cgacgcatgg gccgaagccg tccacgcggc ctgtgacagt gccaatgcgt
      841 ttgggttgga gcagggtggt ccgttgcccg tgtccgagac gaaggccacg gccaagagta
      901 tcgcccgctg ggtgtggcgg cacttcagcc ctgccgagtt ccgcgccatt caagcagcca
      961 gaggggccaa ggggggcagg gtatccaagg gagggggtag acccagcaac agcggcattg
     1021 ctcgtgcaga tctgctgccg gaggtgctac gactcaaggc tcagggttac agcaaccgtg
     1081 atatagccga ggacctgcaa atctcggctg gcagtgtgtc caattatctt cgccgtgatc
     1141 gtgagtgatc aaaacaagcc ttatcagata acagcgcccg cagggcgttg ttatcgccct
     1201 aaatgaatct ttatagagag aggcttggcg aggtgggtaa ctgcgcccgt ccgtccatca
     1261 ggggcttgtc ccctgtgggc ggcagcagtt atccatggag tggtctttgt cttgacggca
     1321 tacgctggca gcgtacaatc aaatctcgat tttgattgtt cgtattaagg atttgattgt
     1381 gcaggaacga gacgccaaga ctgtgttcga ccttgggggc ttctccaaag ccctgatcgc
     1441 ccctaccccg atgggcgcag gctaccatct tcacctggta cgcttcggga agggagggga
     1501 aacagaggtg atcgagcggc agcgcggcgg ctggcgagtc ttcacgacca tcgatgcggc
     1561 cgcgaacacc gcgaaccaga tcggctttcg ccgcatcgag gtagacctca gcagccgccg
     1621 ctaggcggct acatgcccat atcagaagaa gagtctactg ttctgtcccg tagcttccgg
     1681 cccgtctctt tgacctcttt cggccaatct attccatcag cggcctgtgc cgcccttgcc
     1741 gatggcatca gcacgttcac gacccgatct gccagctttg cccatgctcc cgatatccca
     1801 atgctctcta ggccgctctt agctgcatct ttcaactctt ggtcttgtgg tgcttcaact
     1861 attgcgacaa acgcgtctag cacgccccca agggccttca gcgcccgttc ctgaactctt
     1921 gagatggcct catctacata gccctgcgtc tttttaacag ccagctctag ccgacccagc
     1981 agctctgttt gtttccgctc ttcgtctctc agggccctca tcttgtcctc tcccgccctg
     2041 atctcggcgg cctgcgcctc agccttgtcg tgcagctcct tgatacgtgc ctctgcggcg
     2101 tcaatctccg cggctcgctc ggtcgtttgg gtcaggtagt ccagctgctg ctgagcagca
     2161 tgtttatgcc cgtacagggc acttttcttt gatgccttga gaagctgcgc ccgtgtgtag
     2221 agtggttccc ccctccccag aatccccgtt gaatggagag gttcgagcgc atcaatgatc
     2281 tcttccacag ggggcagctt aggttttgag agcggcggtg tactgacctg ttcagcaatg
     2341 agcgatgctg cccgtttcgc ggccttgtag tccttgttcg ggagccgcgc tcgcctcggc
     2401 cctgttcgca gcaggccgaa gcgggctgat atggtatgga tctcgtcctg ccatgcgctc
     2461 atggcctcgc tgtaggcttt ggtctgtaga cccttgcgct ccccccgcgc ataagacgct
     2521 ttctctgcgg cccagccagg atgcaccact ccgtaactct caccaggaag cggcaccagg
     2581 taaaagtgac agtggcgata gggctcatca acatgctcta ccacagaccg aaggcgtttc
     2641 ccgtgctttc cctttaggtg ctcgatcatc gcctcccgat acttttccca gcggtcttct
     2701 gggaagtcct cggggacact gaacaccccg gcagccatgc actgcccatc gatgcgcagc
     2761 ttcttcccct tcggatcttt ggcctgttca gcccactcag ttgccacttt aacgacttcg
     2821 gaaggtgtca tgccaaactg gatgatcggg tctttcacct gctcgatatc aaggtgctta
     2881 taggccccct ttttccgctc tgcctcgtct gctatgtctt gtgccgactt acccccagca
     2941 accttagagc tggctttcct tgcatacgaa tcaaggtgtg cgaactgata accggacata
     3001 gagaccccca tcaaaaatac cgtcatcaga gtaccaccta aataggctgg tgtgggaggg
     3061 ggcatataaa catagcgacc gactatgttt atgcgtttga gtccttcgtc ctcaaacaca
     3121 taaacgcggc cctgcggggc aaaaaactgc cggcaggcgt ttgggatcgt accgagttga
     3181 gatggctgac tgtccatttt acgggggtag accaccggat ctgctccaca ccggttcggc
     3241 ctgtttcggc ctctaaaacc gccctggaat cgtcataaac tttcagcgca aaaaggcgca
     3301 gtgttgaacc gcaacactgc gccttttggg gtgttctggg aggcatctgg ttgggcggca
     3361 agttttcgca tgcgaaaact tcaattgaac ataaaaggcg ttatgcgtac tggtttgagg
     3421 ggggggaggc aatacatttt tgtgttgaat ccgcactcta tgtctgaaaa gataattcca
     3481 caaaaaaaca cttgtaactt aagggtaaat catgaaatta aaatgtttat ttatcacttc
     3541 tggcgcattt ttattgtccg catgcacttc tatatcccaa caggaggctg gtagcatcaa
     3601 ttatggtgat aagcccgtta actacgataa agcaataata tccgcaataa aaaacaacct
     3661 aaaagatcct gattccatga aggttgtttc tatcaccaac ccatatcgcg tatactctaa
     3721 atgcgggttc ggcacagtta agtatgggtg gtataccacc attagataca atgccaaaaa
     3781 ttcatatggt ggatatgtag gagaaaaaga gtttaactat gcttatttaa acggaaagta
     3841 tgatgttggg atgatgtgtt caggtgggtc ttggaatgga aacggcaact caattttatt
     3901 agatgagatt acatatttgt gtaaaggcga ctgccctcaa aattaataaa caagggaagg
     3961 tgcgaacaag tcaccaaacg caaaaaaaac gcctatgtaa gtgattgaat ttaattgact
     4021 aaaagtttca ttttcgttgc ggagggtact tattcgcacc ctccctagat aaaacctaga
     4081 ggctttgatt ttatttggtt tttcgtgcta aaaaagcaaa taacggattt actatgactt
     4141 tgtttataaa gtaaatagca aggttactgt taattaaatc aaagtatatt ccgctctata
     4201 taaatagaat ggagtcaagg atgagcaata tcaatatggt tacgacaaac ttcatctaac
     4261 aaattaataa tctttccatt atgtatctcc taatataaaa agtgattgaa tttaatcacc
     4321 aagaaggtaa tttgtttttg catctaaaaa caaatattta ttaaatctcc ttcttgtttt
     4381 tattatatca aggaggtggg gttgatagca cgagttattg tattttagtt tttaccccta
     4441 cccccccatc gtggaagaca caaagttaat gtgaagtggc ggagagctct tccaagtgct
     4501 tgtcactgcg tataaggggg cgttacgtta aatcggacgc ccttttttgc gcccgccagc
     4561 ctgcaagaca ggttgttttg ctgggcgtga ccgcgcattc tgcaccgcat catcccttgt
     4621 ctcggaattt ccccttatgg acaccatcac tcatcccacc ctcgtccagt tggttgccgc
     4681 cggggccgtc cgtgtcgtgg tcgccgtcgg ccagccaggc ggttggaccc tgctggtgcg
     4741 ctacggtctg gccgaacgcg ccctcgccgc ccagcgcagt aagcagctac gggtctttcg
     4801 caagctcgat accttgcagg cctatttgca ggaaatcggc gtggtccgct ttgagatcga
     4861 tgccgccggg ttcgtggtcg caccacgaca gcggtaacct attgcgataa acatcaattg
     4921 acattacatt tgtaattact gaaatgattg tgaatagtga gggcagtgat gggaacgatc
     4981 aatttgcgca tcgatgatga tctaaaagcc cgttcttatg cggtgctgga tcggttgggg
     5041 gtgacgcctt cagagttgct gcggcaaacg ctggagtacg ttgctgctca ggagcggtta
     5101 ccgtttcgaa ctaccgtcgt gactcaggac gaagaggctc tccttgcgct cgttcgggag
     5161 cggttggcca acccccaaaa agggatcaag gtgtcgctgg atgacctttg agcttgagtt
     5221 tgatccccgt gcttggcggg agtggaagaa actgggcgat accgtgcgcc agcagttcaa
     5281 gaagaagctg gtagaggtgc tggaacggcc aagaaatgag gccaaccgcc tgcatgggct
     5341 gccggattgc tacaaggtca aattgcgtgg tgccggatac cggttggtgt accaggtgca
     5401 agatgatcgg gtggtggtgt ttgtagtggc agtagggaaa cgagagcgtg agcaggtata
     5461 tctggacgca ggttcgcggc tggaataggc gaagttgccg agagggggtt
//
