LOCUS       NZ_AP024952             7378 bp    DNA     circular CON 30-JAN-2024
DEFINITION  Aeromonas caviae strain KAM345 plasmid pKAM345_4, complete sequence.
ACCESSION   NZ_AP024952
VERSION     NZ_AP024952.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00294561
            Assembly: GCF_019972775.1
KEYWORDS    RefSeq.
SOURCE      Aeromonas caviae
  ORGANISM  Aeromonas caviae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Aeromonadales;
            Aeromonadaceae; Aeromonas.
REFERENCE   1
  AUTHORS   Maehana,S., Suzuki,M. and Kitasato,H.
  TITLE     Draft genome sequence of carbapenem-resistant Aeromonas spp. in
            Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7378)
  AUTHORS   Maehana,S., Suzuki,M. and Kitasato,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUL-2021) Contact:Shotaro Maehana Kitasato university,
            Allied Health Sciences; 1-15-1, Kitasato, Minami-ku, Sagamihara,
            Kanagawa 252-0373, Japan URL
            :https://www.kitasato-u.ac.jp/ahs/english/index.htmltmRNA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8.0
            Genome Coverage                   :: 193x
            Sequencing Technology             :: Illumina HiSeq X; ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/30/2024 10:21:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,616
            CDSs (total)                      :: 4,457
            Genes (coding)                    :: 4,339
            CDSs (with protein)               :: 4,339
            Genes (RNA)                       :: 159
            rRNAs                             :: 11, 10, 10 (5S, 16S, 23S)
            complete rRNAs                    :: 11, 10, 10 (5S, 16S, 23S)
            tRNAs                             :: 122
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 30 of 118
            Pseudo Genes (incomplete)         :: 89 of 118
            Pseudo Genes (internal stop)      :: 12 of 118
            Pseudo Genes (multiple problems)  :: 10 of 118
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP024952.1.
            Annotated at DFAST https://dfast.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7378
                     /organism="Aeromonas caviae"
                     /mol_type="genomic DNA"
                     /strain="KAM345"
                     /db_xref="taxon:648"
                     /plasmid="pKAM345_4"
                     /geo_loc_name="Japan:Kanagawa"
                     /collection_date="2018"
     gene            join(7126..7378,1..536)
                     /locus_tag="LDN68_RS22970"
     CDS             join(7126..7378,1..536)
                     /locus_tag="LDN68_RS22970"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_223955020.1"
                     /translation="MHKRKFTTLAFYSLALVLLLGLGLGLHQYYIYKVTREAHKTTVDR
                     NLSLMHMRADGYLERLALDGEFIASTPILRMYLENPSAETLAGAQDALGLLLRCVYLDQ
                     LRYIDQQGMERIRVNQRGGPPQLATELQNKSDRDYVQRGLALTAGGVYFSPIDLNIEHG
                     QIERPFHPILRSVAKVMVNGSPAGMVVQNANVDELEKRLRTELPTAVAQPVVLNSSGGW
                     IMGGGEKDWLFSAKPDAPDALLSTQARAVGKNPGQFVRSI"
                     /name="hypothetical protein"
     gene            1199..1639
                     /locus_tag="LDN68_RS22980"
                     /old_locus_tag="KAM345_044610"
     CDS             1199..1639
                     /locus_tag="LDN68_RS22980"
                     /old_locus_tag="KAM345_044610"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA];
                     GO:0046872 - metal ion binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="WP_268918824.1"
                     /translation="MHHGASARHGRTPKMPPCVLMGDIDFFKKVNDTYGHAAGDEVLKT
                     FTNTIRQLLRKGDFLGRLGGEEFVVALENTELPNAQTMAERLRLAIANSQTPNDGGIPI
                     AITASFGLALYSRDESVDAWLARADAALYRAKQSGRNRVEIA"
                     /name="GGDEF domain-containing protein"
     gene            complement(2097..2525)
                     /locus_tag="LDN68_RS22990"
                     /old_locus_tag="KAM345_044620"
     CDS             complement(2097..2525)
                     /locus_tag="LDN68_RS22990"
                     /old_locus_tag="KAM345_044620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017413093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="WP_128318468.1"
                     /translation="MHNTKPRTVLSLKKAPPQPEITQAAQQSTAIPTKRKIPAAFPCLQ
                     SHWPELFNLKHPTPLKIGILAPVIASLKQQDEPYTDTQIGKAIGWYCRRFEYLKALTNA
                     PHRMGIDGNTFPISDQDRQNAMKQIKARIKEARSCPKR"
                     /name="ProQ/FINO family protein"
     gene            2666..2917
                     /locus_tag="LDN68_RS22995"
                     /old_locus_tag="KAM345_044630"
     CDS             2666..2917
                     /locus_tag="LDN68_RS22995"
                     /old_locus_tag="KAM345_044630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="WP_128318469.1"
                     /translation="MPTVLIIGGWRVVIYPNDHRPAHVHVIGGGCEAVFNLSGDQGGPE
                     LRENYGFDSKALKRIWAELQGNQAHLISQWEVIHGTNR"
                     /name="DUF4160 domain-containing protein"
     gene            2901..3440
                     /locus_tag="LDN68_RS23000"
                     /old_locus_tag="KAM345_044640"
     CDS             2901..3440
                     /locus_tag="LDN68_RS23000"
                     /old_locus_tag="KAM345_044640"
                     /inference="COORDINATES: protein motif:HMM:NF021873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="WP_328706130.1"
                     /translation="MELTDEMIAQANKRGSEARKRSPVATSVRYDRRIGRVVIALSSGL
                     ELAFAPHLAQGLEAAKPSDLQEIEISPSGLGVHFPRLDADLYLPSLLEGLLGSENWAAS
                     KMGAKGGKAKTPLKSQRRGPTALKVGVQEKTCLRRPECGNVLPRKDKPRISVFNLAEES
                     ESSGPFTKNRESISHD"
                     /name="DUF2442 domain-containing protein"
     gene            3480..4367
                     /locus_tag="LDN68_RS23005"
                     /old_locus_tag="KAM345_044650"
     CDS             3480..4367
                     /locus_tag="LDN68_RS23005"
                     /old_locus_tag="KAM345_044650"
                     /inference="COORDINATES: protein motif:HMM:NF015076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_223955023.1"
                     /translation="MVKPALQAADFVERLPRRPYCTDDPAHGLHIRPQATALAYRHVQH
                     NPPPHVSCIVFDVDRKPYEQRREGYQEWRDRDLPAPHWIAINPENGNYHLGYLLAAPVA
                     RTNAARLKPLRYLAAIEHVLAKKLGADMGYVGLITKNPVHRDWWTIWHNHEPYSLDYLA
                     EFCPDADLAAYNRRSGKEASGLGRNVTVFDNVREWGYSAVREYWRPNGYEAWANAVRAA
                     CESANAFGREQGGPLPHSEIKATAKSIARWIWRHLTPTSFADYVDRTHTTEIQARRGAK
                     GGKVSKGGETEQGR"
                     /name="replication initiation protein"
     gene            4381..4488
                     /locus_tag="LDN68_RS23010"
     CDS             4381..4488
                     /locus_tag="LDN68_RS23010"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="WP_223955015.1"
                     /translation="MLELKAQGYSNRDIAEDLGIGSASVSRYLSKISPE"
                     /name="LuxR C-terminal-related transcriptional regulator"
     gene            complement(4640..4930)
                     /locus_tag="LDN68_RS23015"
                     /old_locus_tag="KAM345_044660"
     CDS             complement(4640..4930)
                     /locus_tag="LDN68_RS23015"
                     /old_locus_tag="KAM345_044660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005321770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_128318465.1"
                     /translation="MKDNADKGTGDMFGIKRGRGRPKTGSAKTGAERQAAYRAKQQENN
                     VTVTINRGLLEGLDAQMQAIRDGGSAVVLTSEQAGEILRALRTAENKQLRG"
                     /name="hypothetical protein"
     gene            5123..5461
                     /locus_tag="LDN68_RS23020"
                     /old_locus_tag="KAM345_044670"
     CDS             5123..5461
                     /locus_tag="LDN68_RS23020"
                     /old_locus_tag="KAM345_044670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019820367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_094965905.1"
                     /translation="MPTEQPKRLKTIKVRVTETEWASLRAACPKAQLAEWMREYCLGVV
                     PQQRRTPPPTIDPALLRQVAGIGNNLNQIARRINASPLTTLDKVEYLAALAAVARELEG
                     LRRDRQNP"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5555..6130
                     /locus_tag="LDN68_RS23025"
                     /old_locus_tag="KAM345_044680"
     CDS             5555..6130
                     /locus_tag="LDN68_RS23025"
                     /old_locus_tag="KAM345_044680"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_223955016.1"
                     /translation="MVKELIDGSHYAQRYTSGVLSFHEPDLPRETKDRLMDEFEQALLP
                     GLDADQYAVLWVEHRDKDRLELNFVVPNVELLSGKRLQPYFHGADGHRINAWRTAINGE
                     LGLHDPDDPINRQAVTTAKDLPGTSWKPNKPLQAACWAWLRRGRQKPGGRSQDPDRGGV
                     RRYQDNQEQHQHCRPRRGPEYPPKGGDL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            6127..6561
                     /locus_tag="LDN68_RS23030"
                     /old_locus_tag="KAM345_044690"
     CDS             6127..6561
                     /locus_tag="LDN68_RS23030"
                     /old_locus_tag="KAM345_044690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_223955017.1"
                     /translation="MSKILDMAKTFEQTSKQQADDIESSLKPEFERHERAIKEALDSSR
                     QSINDAIQDQQKLIGWLLLKSWGVMLISALLLLVATSGTLWYQGNLIAERQATLEALGK
                     KGGKLIFNKCEDENGKHRFCIRMNVDEGKFGDDYMIPKGY"
                     /name="MbeB family mobilization protein"
     gene            6565..6717
                     /locus_tag="LDN68_RS23035"
     CDS             6565..6717
                     /locus_tag="LDN68_RS23035"
                     /inference="COORDINATES: protein motif:HMM:NF016769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD/MobD family mobilization/exclusion protein"
                     /protein_id="WP_223955018.1"
                     /translation="MTELERHLLNALETLQSEQEQQWRSLQTACDSLLGLYETTKKKTR
                     VCREK"
                     /name="MbeD/MobD family mobilization/exclusion protein"
     gene            6734..7024
                     /locus_tag="LDN68_RS23040"
                     /old_locus_tag="KAM345_044700"
     CDS             6734..7024
                     /locus_tag="LDN68_RS23040"
                     /old_locus_tag="KAM345_044700"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_223955019.1"
                     /translation="MRALEQAGQQLGNAGRQLEGVIAQQTAQTQEGLLDEYQQAHAERN
                     LYFNRKHEQKEISGLPIDKAREYLQEGLEGRIERQQARDSGLEGRAEPIYH"
                     /name="hypothetical protein"
     Typing          3481..4431
                     /name="rep_cluster_1010"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.681"
                     /coverage="95.0"
                     /evalue="0.0"
     Typing          5986..6746
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="87.405"
                     /coverage="98.0"
                     /evalue="1.26e-111"
ORIGIN
        1 cactggcggg cgcgcaggac gctctcggac tcttgcttcg ctgcgtctat ctggatcaac
       61 tgcgctacat cgatcagcag ggcatggagc ggattcgggt gaatcaacga ggtgggcctc
      121 ctcaattggc cacagagcta caaaacaaat ctgaccgtga ttacgttcag agaggcttgg
      181 cactcacggc tggcggcgtc tatttctcgc caattgatct gaacattgaa cacggacaga
      241 tcgagcgccc cttccacccc atactgcgtt cggtggccaa ggtcatggtc aacggcagcc
      301 ctgcgggcat ggtggtgcag aatgccaatg tggatgaact ggaaaaacgg ttgcgcacgg
      361 aactgccaac ggcggtggcg caaccggtgg tgctcaacag cagcggcggc tggatcatgg
      421 gcggtggtga gaaggactgg ctgttttcgg caaagcctga tgccccggac gctctgctat
      481 ccacacaagc ccgcgctgtg ggaaaaaatc caggccagtt cgtcaggtca atttgagtat
      541 cgtggggaat gccactatta cgcttggtac caattcaagc agcagcaatg gcaggcagag
      601 cgctggctgg tagctcagcg ctctgccggt caagcctgcg gacacttgac cgcttcggca
      661 gttaagacgt gggccacgca actggtggta atgtcgactt tcaccgttcc gctgttggtg
      721 ttgtggctgt tgtcccgctg gaacgcgcgc gaactacggc gtggacttag agagacgaac
      781 tcccagctag aactcatcac tagcgaagct gacgttgccc tgttgatggt cgaccatgag
      841 tgccgcgtct gctggattaa ccctgaatcc gaacgcctat tgggctggaa gaccgacgag
      901 ttggttggtg caaacttgca tgaaagaacc cacatacgga acggggagga tctgcactcc
      961 gggcctgtcc gacgctgcag gcgcttcaaa caggacagcg ttaccgcaat gatcgtgacc
     1021 gagtgctcgc gaagtctggc gaaattttga atgtaagcat gcgtgtgagt ccgtttggtg
     1081 agggtgaagc gcgcaatgcc atcgtgacga ttgccgacgt aggggacgca gtggaccgag
     1141 agcagcgttt gacacggctt gccacgactg atccattgac cggcgcactc aaccgtcgat
     1201 ccatcatgga gcgtctgcaa gacatggtcg aacaccaaag atgccacctt gcgtgttgat
     1261 gggcgatatc gattttttca agaaagtcaa cgacacctat ggacacgctg cgggcgatga
     1321 ggtactcaaa actttcacca acacgatacg tcagttgctg cgcaagggtg actttttagg
     1381 tcggctcggc ggtgaagagt tcgtggtggc cttggagaac accgagctgc caaacgccca
     1441 aaccatggcc gaacgcctgc gcctagccat tgcaaatagc caaaccccaa acgatggcgg
     1501 tattcccatc gcaatcaccg ccagctttgg cttggcgctg tacagccgtg acgaatcggt
     1561 ggatgcgtgg ctggcccgcg cagatgctgc actgtaccgc gccaaacaat cggggcgaaa
     1621 ccgggtggaa atagcttagc gctgctgggg tgctgtttcg caggatgccc tgcagacgca
     1681 gatcacgccc attcggccgg aaatcctcgc gcctatcgca tccgacggaa atctacctgc
     1741 tcgcattcat tgcgattgcg tttccacaca gcctcggtcg ggtgccgtcg cgtgcgcagt
     1801 gtcaaagctg acatgacgaa tacatgagat gggctgatgc tcgccgtccg gtcaagctgt
     1861 tgagtcgccc cgggttctac cctgttttca cggacacatc taaaagaggc ataatgtgcc
     1921 tgtaaggagt gttcatgacc aagcgaagaa aattcagtcc agagttcaag cgagaagctg
     1981 tcaaactgac ccagcagccg ggaacggggg cggggctttg agatgtagct gccgcgctgc
     2041 gcaccgactc ccaaacgaag cgcgacagac tgcgctagtc gctcttgttt gggagttcat
     2101 cgtttggggc aggatctagc ctctttgatg cgcgccttga tttgtttcat cgcgttttgg
     2161 cggtcttggt cgctgatggg gaatgtgttg ccatcaatcc ccattcgatg aggagcattg
     2221 gtgagggctt tgaggtactc aaatctacgg caataccagc caatggcctt cccaatctga
     2281 gtatcggtgt aaggctcgtc ctgttgcttc aggctggcga tgacaggagc taggattccg
     2341 attttaagtg gagtgggatg tttgaggttg aatagctcgg gccagtgcga ttgtaggcaa
     2401 gggaaagccg ctgggatttt tctcttcgta ggtatcgcgg tggactgttg tgctgcttgg
     2461 gttatctcag gctgtggtgg ggccttttta agactcagta ctgtacgggg cttggtgttg
     2521 tgcatggacg tatacctgcg gaaatgatgt ttgcaggcaa gttatcgaaa cccccatcag
     2581 gatgcaaaaa cagttgaact atcgataatg cacccattcg agtgtattat tgcgtttata
     2641 gaaaccaaaa cagttaggtt gtttgatgcc aacggtgttg atcattggtg gctggcgagt
     2701 ggtgatttat ccaaacgacc atcgtccggc gcacgtacac gtgattgggg gcggctgcga
     2761 ggcggtgttt aatctctcgg gtgatcaagg cgggcctgag ctgcgggaaa actacggatt
     2821 tgatagtaaa gcgctgaaac ggatctgggc agagttgcaa ggcaatcaag ctcacctgat
     2881 aagccaatgg gaggtgatcc atggaactaa ccgatgaaat gatcgcacaa gcaaataaac
     2941 gcggttctga agcgcgtaag cgtagtcctg ttgctacgag cgtgcgttat gaccggagga
     3001 ttggacgggt cgttatcgcc ttgtcttctg gtttggaact ggcctttgcc cctcatctgg
     3061 cacaggggtt ggaggccgcg aagcctagcg acttacagga gatcgagatc tctccgtcag
     3121 ggttgggagt gcactttccg aggttggatg cagatctcta cttaccttcc ctgctggaag
     3181 ggttgttggg ctctgagaac tgggcggcat ccaagatggg ggcgaagggc ggcaaggcaa
     3241 aaaccccgct aaagtcgcag cggcgcgggc caacggcgct aaaggtgggc gtccaagaaa
     3301 aaacgtgcct gcggcggcct gagtgcggta acgtattgcc cagaaaggac aaaccccgaa
     3361 tctctgtatt caacttggcg gaagagtcag agagttcggg gccgttcacg aagaacagag
     3421 aaagcatatc acatgactga cctcacacaa gactgcaccg ccaataagcg cggtgatcga
     3481 tggtaaaacc agcgcttcaa gccgctgact ttgtcgaacg tctcccccgc cgcccctact
     3541 gcaccgatga tccggctcat gggctgcaca ttcgcccgca agcaaccgcg ctggcctatc
     3601 gccatgttca gcacaacccg ccgccgcatg tctcctgcat tgtgtttgat gtggatagaa
     3661 agccctatga gcaacggcga gaggggtatc aagagtggcg agatcgcgac ttgcctgcgc
     3721 cgcattggat cgcgatcaac cccgaaaacg gcaattacca cctcggctat ctgctggctg
     3781 cgccagtggc gcgtaccaat gcagccaggc taaagccgct gcgctatctg gcggccatag
     3841 agcacgttct ggcgaagaag ctgggtgctg acatggggta tgttgggttg atcaccaaaa
     3901 acccggtaca tcgcgattgg tggacaatat ggcacaacca tgagccctat tccctcgact
     3961 atctcgctga gttctgccca gatgcggatt tagcggccta caaccgccgt agcggcaaag
     4021 aggcgagcgg gctggggcgt aacgtcacgg tgtttgataa cgtgcgggaa tggggctaca
     4081 gcgcggtgcg ggagtattgg cgccctaacg ggtacgaagc atgggccaat gcggtgagag
     4141 ctgcttgtga gagcgccaat gcgtttggac gcgagcaggg cggcccgtta ccccatagtg
     4201 agatcaaggc cactgccaag agcatcgccc gttggatctg gcggcacctt accccgacct
     4261 cgtttgcgga ttacgttgat cggacacata ccaccgagat ccaagcgcgg agaggggcta
     4321 agggcggcaa ggtatccaag ggcggggaga ccgagcaagg ccgctgatct tctgcctgcg
     4381 gtgttagaac tcaaggcgca gggctacagt aaccgcgaca ttgccgagga tttagggatc
     4441 ggttcggcaa gtgttagccg ctatctctcc aaaatctcgc cagagtgatc caaaacaagc
     4501 ctatatcaga taacagcgcc cgaagggcgc ttcttgtctc tagagaatca ttcaagagag
     4561 aggatcttgg cgaggtggtt aagcccgatc ccgttgggct gtccatcagg ccatgtgttc
     4621 ggcctgtggg cagtagctgt tatccccgga gctgtttgtt ctctgctgtt cgtagcgccc
     4681 gcaagatctc cccggcctgt tccgaggtca gtaccaccgc tgaacccccg tctctgatcg
     4741 cctgcatctg ggcatccagt ccttccaata gccctctatt tatcgtcaca gtaacgttat
     4801 tttcctgctg ttttgcccgg taggcagctt gtcgttctgc ccctgttttg gctgatccgg
     4861 ttttgggtct accccgtccg cgcttgatgc caaacatgtc tccggttccc ttatctgcgt
     4921 tgtctttcat cgtccaaatt cccgtcacag tgaccataaa tattatttgt cactgtgaca
     4981 caaaaagcat tcatccgcac cctcaaaatt caacccatgt taaagtcata agcaaggaca
     5041 cacaggggcc gcttcgcggt ctatcccctg tggccttgtt caggggcaag ccccgaaacc
     5101 cccgaaaaaa cggaggccat cgatgccgac agagcagcca aaacggctca aaacgatcaa
     5161 ggtcagagtc accgaaaccg agtgggccag cttgcgggcg gcctgcccca aagcccagtt
     5221 ggcggagtgg atgcgcgagt actgcttggg ggttgttccc cagcagcggc gcaccccacc
     5281 gccaaccatc gatccggcct tgctccggca ggtggccggg atcggtaaca acctcaacca
     5341 gatcgcccgg cgcatcaatg ccagcccgct gaccacgttg gacaaggtgg aataccttgc
     5401 ggcgttagcc gccgtggccc gagaactgga ggggctacgc cgtgatcgtc aaaatccata
     5461 gccggggagt cggtcgcgga tctggtccgg tggaatacct gcttggcaaa gacgggaacc
     5521 ggacggggcc acgctggatc gcggcgaccc cgagatggtc aaggagttga tagacggcag
     5581 ccattacgcc cagcggtaca ccagcggcgt gctgtcgttc catgaaccgg acttgccgag
     5641 ggagaccaag gatcggttga tggacgagtt cgagcaagcc ctgttgcccg gactggacgc
     5701 cgatcagtat gcggtgctgt gggtcgagca ccgggacaaa gaccgcctag agctgaattt
     5761 cgtggtgccg aacgtcgaac tgttgtccgg caaacgcctt cagccgtatt tccacggggc
     5821 ggacggccac cggatcaacg catggcgaac ggccatcaat ggcgagttgg gattgcacga
     5881 cccggacgac ccgatcaatc ggcaggccgt aaccacggcc aaagatctcc ccggaacaag
     5941 ctggaagccg aacaagccat tacaggcggc ttgttgggca tggctgcggc ggggccgtca
     6001 aaaaccggga ggacgttctc aagaccctga ccggggcggg gttcgacgtt accaggacaa
     6061 ccaagaacag catcagcatt gccgaccccg aagggggccg gaatatccgc ctaaaggggg
     6121 cgatctatga gcaagatttt agacatggca aagaccttcg agcagacatc gaagcagcaa
     6181 gccgacgata tagagagcag tctgaagcca gagttcgaga ggcacgagcg agctatcaaa
     6241 gaagcgttgg actcaagcag gcagagcatc aacgacgcca tccaagacca gcagaagctg
     6301 atcggctggc tcctgctcaa gagctgggga gtgatgctga tctcggcctt actgttgctg
     6361 gtcgcgacga gcgggacgct gtggtatcag gggaacctga tcgccgagcg gcaggccacc
     6421 ttggaggcgt tgggcaaaaa gggcgggaaa ttgatcttca acaagtgcga ggacgagaac
     6481 gggaagcacc ggttttgcat caggatgaac gtggacgaag ggaagttcgg ggacgactac
     6541 atgataccaa agggctacta aacgatgacc gagctagaac ggcacttatt gaacgcgcta
     6601 gagaccttgc agagcgagca agagcagcaa tggcgcagct tgcaaacagc gtgcgacagc
     6661 ttgctggggc tgtacgagac gacgaaaaag aaaacgcgag tttgcaggga gaagtgagag
     6721 ccttgcgcca gcagtgcgag cacttgagca agcaggtcag cagcttggca acgcaggtcg
     6781 ccagcttgaa ggcgtgatcg cgcagcagac ggcgcagacg caggaaggtt tgttggacga
     6841 ataccagcag gcccacgcag agcggaacct gtatttcaat cggaagcatg agcagaaaga
     6901 gatctccggc ttgccgatag acaaggccag agagtaccta caggaaggac tggaggggcg
     6961 gatagagcgg cagcaggccc gcgattcggg attggaaggc cgcgctgaac cgatttacca
     7021 ttgagttcgg agaccgtatc tgcgtcaatt gaagtccgaa ccgtttacac aaaaattcgg
     7081 acacgccctg tgtgagtcct caaaatctcc ggaaagacaa cgttcatgca caaacgaaaa
     7141 tttaccactc tcgccttcta ttcgttagcg ttggtgcttt tgctgggact gggtctcggc
     7201 ttgcaccagt actacatcta caaggtcact cgtgaggcgc ataaaaccac ggttgatcgc
     7261 aatctgtcgt tgatgcatat gcgggctgac ggctatctgg aacggttggc actggatgga
     7321 gaattcattg cgagcacccc catcctgcgc atgtatcttg aaaacccctc ggcagaaa
//
