LOCUS       NZ_AP024947             4897 bp    DNA     circular CON 30-JAN-2024
DEFINITION  Aeromonas caviae strain KAM339 plasmid pKAM339_7, complete sequence.
ACCESSION   NZ_AP024947
VERSION     NZ_AP024947.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00294556
            Assembly: GCF_019973835.1
KEYWORDS    RefSeq.
SOURCE      Aeromonas caviae
  ORGANISM  Aeromonas caviae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Aeromonadales;
            Aeromonadaceae; Aeromonas.
REFERENCE   1
  AUTHORS   Maehana,S., Suzuki,M. and Kitasato,H.
  TITLE     Draft genome sequence of carbapenem-resistant Aeromonas spp. in
            Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4897)
  AUTHORS   Maehana,S., Suzuki,M. and Kitasato,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUL-2021) Contact:Shotaro Maehana Kitasato university,
            Allied Health Sciences; 1-15-1, Kitasato, Minami-ku, Sagamihara,
            Kanagawa 252-0373, Japan URL
            :https://www.kitasato-u.ac.jp/ahs/english/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8.0
            Genome Coverage                   :: 323x
            Sequencing Technology             :: Illumina HiSeq X; ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/30/2024 10:20:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,780
            CDSs (total)                      :: 4,625
            Genes (coding)                    :: 4,536
            CDSs (with protein)               :: 4,536
            Genes (RNA)                       :: 155
            rRNAs                             :: 11, 10, 10 (5S, 16S, 23S)
            complete rRNAs                    :: 11, 10, 10 (5S, 16S, 23S)
            tRNAs                             :: 118
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 29 of 89
            Pseudo Genes (incomplete)         :: 71 of 89
            Pseudo Genes (internal stop)      :: 9 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP024947.1.
            Annotated at DFAST https://dfast.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4897
                     /organism="Aeromonas caviae"
                     /mol_type="genomic DNA"
                     /strain="KAM339"
                     /db_xref="taxon:648"
                     /plasmid="pKAM339_7"
                     /geo_loc_name="Japan:Kanagawa"
                     /collection_date="2018"
     gene            complement(join(<4858..4897,1..812))
                     /locus_tag="LDN97_RS23855"
                     /pseudo=""
     CDS             complement(join(<4858..4897,1..812))
                     /locus_tag="LDN97_RS23855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000635827.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(793..1131)
                     /locus_tag="LDN97_RS23860"
                     /old_locus_tag="KAM339_046210"
     CDS             complement(793..1131)
                     /locus_tag="LDN97_RS23860"
                     /old_locus_tag="KAM339_046210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019820367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_094965905.1"
                     /translation="MPTEQPKRLKTIKVRVTETEWASLRAACPKAQLAEWMREYCLGVV
                     PQQRRTPPPTIDPALLRQVAGIGNNLNQIARRINASPLTTLDKVEYLAALAAVARELEG
                     LRRDRQNP"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1330..1620
                     /locus_tag="LDN97_RS23865"
                     /old_locus_tag="KAM339_046220"
     CDS             1330..1620
                     /locus_tag="LDN97_RS23865"
                     /old_locus_tag="KAM339_046220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005321770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_094965911.1"
                     /translation="MKDNADKGTGDMFGIKRGRGRPKTGSAKTGAERQAAYRTKQQENN
                     VTVTINRGLLEGLDAQMQAIRDGGSAVVLTSEQAGEILRALRTAEKKQLRG"
                     /name="hypothetical protein"
     gene            complement(1773..2759)
                     /locus_tag="LDN97_RS23870"
                     /old_locus_tag="KAM339_046230"
     CDS             complement(1773..2759)
                     /locus_tag="LDN97_RS23870"
                     /old_locus_tag="KAM339_046230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005321767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_094965910.1"
                     /translation="MTEKAFLRNLPHRPYCTDDKSSGLLIRPQATALGYRHIQYNPPPH
                     VASLVFDLDKPDSYHAWQDAGLPAPHWISVNRTNGHAHIGYMLATPVARTSAARQKPLR
                     YLAAIEHVLAKRLGADMGYAGLITKNPTHSDWLTIWHQIEPYSLDYLAEFCPDADLAAY
                     SRRSRKEATGLGRNVTVFDNVREWAYTAIRAHWRPNGYDAWLCAVQAACECTNVFGLEQ
                     GGPLPHSEIKATAKSIARWTWRNLTPSTFADYVDRTHTPEIQAVRGAKGGRIGGKVSKG
                     GGRPSKAASLLPVVLQLKAQGYSNRDIAEDLQISAGSVSNYLRRDRE"
                     /name="replication initiation protein"
     gene            complement(2921..3352)
                     /locus_tag="LDN97_RS23875"
                     /old_locus_tag="KAM339_046240"
     CDS             complement(2921..3352)
                     /locus_tag="LDN97_RS23875"
                     /old_locus_tag="KAM339_046240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019535530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="WP_094965909.1"
                     /translation="MNIPTHLKHKPVLEVINYDQIDGPYANDSDAMGLSIGIAQWNSPG
                     WTDLSAKVWRHTGEKWSRQSEELPLHRVIDLTTLICIAMEYCRNGRLSSSDDFPVSRST
                     DNADLGHHVELMKKELKSGSKYLDESLKRLSLELKRLGY"
                     /name="DUF6530 family protein"
     gene            complement(3467..3775)
                     /locus_tag="LDN97_RS23880"
                     /old_locus_tag="KAM339_046250"
     CDS             complement(3467..3775)
                     /locus_tag="LDN97_RS23880"
                     /old_locus_tag="KAM339_046250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="WP_094965908.1"
                     /translation="MQFTIYRNKGNARAYPYLLDVQSDIIGELATRIVIPLFPLAAFTG
                     RPAQRLNPVIIVEGDKYLVMTQDMAAVRQTQLGEEVTGVQESRQLIKDAIDFLMDGV"
                     /name="CcdB family protein"
     gene            complement(3775..4014)
                     /locus_tag="LDN97_RS23885"
                     /old_locus_tag="KAM339_046260"
     CDS             complement(3775..4014)
                     /locus_tag="LDN97_RS23885"
                     /old_locus_tag="KAM339_046260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="WP_094965907.1"
                     /translation="MVAAAMRSKRTVSVTLEPSLLERARAAGINLSATLAAALQHEISG
                     SEAERWKRENSAAIQELNRITEEHGLLSDDYRAF"
                     /name="type II toxin-antitoxin system CcdA family
                     antitoxin"
     gene            complement(4350..4583)
                     /locus_tag="LDN97_RS23890"
     CDS             complement(4350..4583)
                     /locus_tag="LDN97_RS23890"
                     /inference="COORDINATES: protein motif:HMM:NF016769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD/MobD family mobilization/exclusion protein"
                     /protein_id="WP_153913308.1"
                     /translation="MTELERHLLNALETLQSEQDRQLSGLQTACESLLRQFETTKKENA
                     SLLGEVKVLRQQCEHLNEQVSSLATQVASLKA"
                     /name="MbeD/MobD family mobilization/exclusion protein"
     Typing          complement(4..272)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.556"
                     /coverage="99.0"
                     /evalue="1.09e-45"
     Typing          complement(4142..4896)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="87.352"
                     /coverage="99.0"
                     /evalue="4.91e-118"
ORIGIN
        1 ccaacgcttc tttgatagct cgctcgtgcc tctcgaactc tggcttcaga ctgctctcta
       61 tatcgtcggc ttgctgcttc gatgtctgct cgaaggtctt tgccatgtct aaaatcttgc
      121 tcatagatcg ccccctttag gcggatattc cggccccctt cagggtcggc aatgctgata
      181 ctgttcttgg ttgtcctggt aacgtcgaac cccgccccgg tcagggtctt gagcacgtcc
      241 tcccggtttt taaccagccc cgccgcagcc atgcccagca agccgcctgt aatggcttgt
      301 tcggcttcca gcttgttccg gggtaggtct ttggccgtag tcacggcctg ccgattgatt
      361 gggtcgtctg ggtcgtgcaa tcccaagtcg ccattgatgg ccgttcgcca tgcgttgatc
      421 cggtggccgt ccgccccgtg gaaatacggc tgaaggcgtt tgccggacaa cagttcgacg
      481 ttcggcacca cgaaattcag ctctaggcgg tctttgtctc ggtgctcgac ccacagcacc
      541 gcatactgat cggcgtccag tccgggcaac agggcttgct cgaactcgtc catcaaccga
      601 tccttggtct ccctcggcaa gtccggttca tggaacgaca gcacgccgct ggtgtaccgc
      661 tgggcgtaac ggctgccgtc tatcaactcc ctgaccatct cggggtcgcc gcgatccagc
      721 gtggccccgc tccggttccc gtctttgcca agcaggtatt ccaccgggcc agatccccga
      781 ccgactcccc ggctatggat tttgacgatc acggcgtagc ccctccagtt ctcgggccac
      841 ggcggctaac gccgcaaggt attccacctt gtccaacgtg gtcagcgggc tggcattgat
      901 gcgccgggcg atctggttga ggttgttccc gatcccggcc acctgccgga gcaaggccgg
      961 atcgatggtt ggcggtgggg tgcgccgctg ctggggaaca acccccaagc agtactcgcg
     1021 catccactcc gccaactggg ctttggggca ggccgcccgc aagctggccc actcggtttc
     1081 agtgactctg accttgatcg ttttgagccg ttttggctgc tctgtcggca tcgatggcct
     1141 ccgttttttt cgggggtttc ggggggcttg ccccctgaac aaggccacag gggatagacc
     1201 gcgaagcggg ccccctgtgt gtccttgctt agtactttaa catgggttgg attttgaggg
     1261 tgcggattaa tggtttttgt gtcacagtga cagataatat ttatggtcac tgtgacggga
     1321 attaggacga tgaaagacaa cgctgataag ggaaccggag acatgtttgg catcaagcgc
     1381 ggacggggta gacccaaaac cggatcagcc aaaacagggg cagaacgaca agcggcctac
     1441 cggacaaaac agcaggagaa taacgttact gtgacgataa atagagggct attggaagga
     1501 ctggatgccc agatgcaggc gatcagagac gggggttctg cggtggtgct gacctcggaa
     1561 caggccgggg agatcttgcg ggcgctacga acagcagaga aaaaacagct ccggggataa
     1621 cagctactgc ccacaggccg aacacatggc ctgatggaca gccctacggg atcgccgtta
     1681 ccccccctcg ccaagatcct ctctcttgaa tgcttttagg gctcaaggaa cgccctacgg
     1741 gcgctgttat ctgatatagg ctttgttttt gatcactcac gatcacggcg gaggtagttg
     1801 gacacactgc cagccgaaat ttgcagatcc tccgcaatat cgcgattgct gtaaccctgc
     1861 gccttgagtt gtagcaccac aggtaggaga gaagcagctt tgctcggcct gcctccgccc
     1921 ttggatacct tgccaccgat acgcccgccc ttagcccctc tgacggcctg aatctctggt
     1981 gtatgcgtcc gatcaacata atccgcgaac gtggacgggg ttagattccg ccacgtccaa
     2041 cgggcgatgc tcttggccgt ggccttgatc tcgctatggg gcaacgggcc accctgctcc
     2101 aagccaaaca cgttggtgca ctcacaggca gcttgcacgg cacagagcca tgcatcgtac
     2161 ccattaggcc gccaatgcgc tctaatcgcc gtgtaggccc attcccgcac gttgtcgaac
     2221 acagtgacgt tacggcctag cccagtcgcc tctttgcgac tgcggcggct gtaggccgcc
     2281 aaatcggcat ccgggcagaa ctctgcgaga tagtcgaggc tgtagggctc gatttggtgc
     2341 cagatggtga gccaatcgct atgggtgggg tttttggtga tcagccccgc ataccccatg
     2401 tcggccccca gccgcttcgc tagaacgtgt tctatggcgg ctaggtatcg cagcggcttt
     2461 tgccgggcgg cgctggtacg ggctactggg gtcgccagca tgtacccaat atgggcgtgg
     2521 ccgttcgtgc ggttaacgct gatccagtgc ggggctggca agcctgcatc ctgccaagcg
     2581 tggtaactgt ccggtttatc caagtcgaac accaaagacg ccacatgcgg cggtgggttg
     2641 tactggatat ggcgatagcc cagtgcggta gcctgcgggc gaatgagcag ccctgacgat
     2701 ttatcgtcag tgcaataggg ccgatggggg agattcctga gaaaggcttt ttctgtcatc
     2761 gatcaccgcg cttattggcg gtgcagggtt gtttggtgtg atccatgtgg tagacttctc
     2821 ttgtgattcg tcacagcccc cgtttttcgc agatccgcca agattccagc gataacgggg
     2881 gtttgtcttt ttatgagcaa taagttaccg catagccccg ttagtaaccc agccttttta
     2941 gctctaaaga cagtcgtttt agactttcat cgagatattt actcccgctc ttgagctctt
     3001 ttttcattag ctcaacatga tgccccaaat cagcgttatc agtagacctg ctaaccggga
     3061 aatcgtcgct agatgacaag cgaccattgc gacagtattc catcgcgata catatcaatg
     3121 tcgttaagtc gataacacga tgcagtggta gttcttctga ctgtcttgac catttctcgc
     3181 ctgtatgacg ccatactttg gctgagaggt cagtccatcc aggtgagttc cattgtgcga
     3241 tgccaattga cagtcccatt gcatcagagt cattagcata tggaccatca atctggtcat
     3301 agttaataac ttccagtacc ggcttgtgct tcaaatgagt tggaatgttc atagcgagcc
     3361 ccagattatc aagtttacta atttcacaaa cattactgag tttagttggt aatgtctatg
     3421 gggttgtacg ccggaaccgc cgaaatatct gcctcccttt aaagcctcaa acaccatcca
     3481 tcaggaaatc tattgcgtcc ttgatgagct gacgagactc ctgcactcct gtgacttctt
     3541 ccccaagctg cgtctgcctc accgctgcca tgtcctgcgt catcaccaga tacttatccc
     3601 cctcaacgat aattaccgga ttcagccgtt gcgctggccg cccggtgaag gccgccagcg
     3661 ggaacagtgg gatcactatt ctggtggcca gctcgccgat gatgtcgctt tgcacatcca
     3721 gcagataggg gtaagcgcgg gcattgccct tgttgcggta gatcgtgaac tgcatcagaa
     3781 cgccctgtaa tcgtcggaaa gcaaaccgtg ctcctcggtg atgcggttaa gctcctgtat
     3841 ggctgcacta ttctcacgct tccagcgttc tgcttctgat ccgctgattt catgctgtag
     3901 tgcggctgca agggtggcgg acaggttgat ccccgccgct ctggctcgtt ccagcaagct
     3961 gggctccagc gtgacgctga ccgttctctt gctgcgcatt gctgctgcga ccatgatgac
     4021 ctccaataaa ttaacacgtg cgatagcacg tgtatgataa tatcacgccc ttgcgacggg
     4081 tgcgactagc gaagtctgtc gcgctcgggg caggggccgc caagcgcagc gcggcagcta
     4141 catctcaaag ccacgccccc gttcgcggac ctgctgccgc tctatccgcc cctccagtcc
     4201 ttcctgtagg tactctctgg ccttgtctat cggcaagccg gagatctctt tctgctcgtg
     4261 cttccgattg aaatagagat cccgttctgc gtgggcctgc tggtattcgt ccaataaccc
     4321 ctcctgcgtc tgcgccgtct gctgcgcgat catgccttca agctggcgac ctgcgttgcc
     4381 aagctgctga cctgctcgtt caagtgctcg cactgctggc gcaagacttt cacttctccc
     4441 agcaagctcg cgttttcttt tttcgtcgtc tcgaactgcc gtagcaagct ctcgcacgct
     4501 gtttgcaagc cgctcaattg ccgatcttgc tcgctctgca aggtctctag cgcgttcaat
     4561 aagtgccgtt ctagctcggt catcgtttag tagcccttcg gtatcatgta atcgtccccg
     4621 aacttccctt cgtccatgtt catcctgatg caaagccgga tcttcccgtt ctcgtcctcg
     4681 cacttgttga agatcaattt cccgcccttt ttgcccaacg cctccaaggt ggcctgccgc
     4741 tcggcgatca ggttcccctg ataccacagc gtcccgctcg tcgcggccag caacagcaag
     4801 cccgagatca acatcactcc ccagctcttg agcaggagcc agccgatcag cttctgctgg
     4861 tcttggatgg cgtcgttgat gctctgcctg cttgagt
//
