LOCUS       NZ_AP024944             8541 bp    DNA     circular CON 30-JAN-2024
DEFINITION  Aeromonas caviae strain KAM339 plasmid pKAM339_4, complete sequence.
ACCESSION   NZ_AP024944
VERSION     NZ_AP024944.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00294556
            Assembly: GCF_019973835.1
KEYWORDS    RefSeq.
SOURCE      Aeromonas caviae
  ORGANISM  Aeromonas caviae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Aeromonadales;
            Aeromonadaceae; Aeromonas.
REFERENCE   1
  AUTHORS   Maehana,S., Suzuki,M. and Kitasato,H.
  TITLE     Draft genome sequence of carbapenem-resistant Aeromonas spp. in
            Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8541)
  AUTHORS   Maehana,S., Suzuki,M. and Kitasato,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUL-2021) Contact:Shotaro Maehana Kitasato university,
            Allied Health Sciences; 1-15-1, Kitasato, Minami-ku, Sagamihara,
            Kanagawa 252-0373, Japan URL
            :https://www.kitasato-u.ac.jp/ahs/english/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8.0
            Genome Coverage                   :: 323x
            Sequencing Technology             :: Illumina HiSeq X; ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/30/2024 10:20:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,780
            CDSs (total)                      :: 4,625
            Genes (coding)                    :: 4,536
            CDSs (with protein)               :: 4,536
            Genes (RNA)                       :: 155
            rRNAs                             :: 11, 10, 10 (5S, 16S, 23S)
            complete rRNAs                    :: 11, 10, 10 (5S, 16S, 23S)
            tRNAs                             :: 118
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 29 of 89
            Pseudo Genes (incomplete)         :: 71 of 89
            Pseudo Genes (internal stop)      :: 9 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP024944.1.
            Annotated at DFAST https://dfast.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8541
                     /organism="Aeromonas caviae"
                     /mol_type="genomic DNA"
                     /strain="KAM339"
                     /db_xref="taxon:648"
                     /plasmid="pKAM339_4"
                     /geo_loc_name="Japan:Kanagawa"
                     /collection_date="2018"
     gene            complement(380..781)
                     /gene="mobC"
                     /locus_tag="LDN97_RS23705"
                     /old_locus_tag="KAM339_045930"
     CDS             complement(380..781)
                     /gene="mobC"
                     /locus_tag="LDN97_RS23705"
                     /old_locus_tag="KAM339_045930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005321773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_128274291.1"
                     /translation="MTKPELKTTTASLRLDPATLAKWRKGACDEGLTLSDWLRAKVDAD
                     QQTHIAPARSRRERDPRHLADPALMRQLAALGSNLNQIARAVNECRLVGSPVYLVELLA
                     LLRSIEEAAGTLLPQLPPPPNREGDGHDD"
                     /name="mobC"
     gene            994..1284
                     /locus_tag="LDN97_RS23710"
                     /old_locus_tag="KAM339_045940"
     CDS             994..1284
                     /locus_tag="LDN97_RS23710"
                     /old_locus_tag="KAM339_045940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005321770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_128274289.1"
                     /translation="MKDTKDQGTGDMFGIKRGRGRPKTGSAKTGAERQAAYRAKQQDNN
                     VTVTINRALVEGLDAQMQVIRDGGSAVVLTPEQAGEILRALRTAENKQLRG"
                     /name="hypothetical protein"
     gene            complement(1434..2378)
                     /locus_tag="LDN97_RS23715"
                     /old_locus_tag="KAM339_045950"
     CDS             complement(1434..2378)
                     /locus_tag="LDN97_RS23715"
                     /old_locus_tag="KAM339_045950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_042650711.1"
                     /translation="MNRAQLDLFRERLPRKPYHTDEFTTGLSIADVSRALGARYIQPNG
                     PTHRHWLVFDVDHAAATLSWDDVGAPAPNITVTNRANGHAHLIYGLETPIRTAPDGKVA
                     PLRYAAAIEAALREKLGADLGYSGLICKNPLHTHWLVQVWEPASYDLSWLADYLDLSPY
                     NGRKQLPAYGLGRNCTLFEKTRQWAYKAIRQGWPGYEAWLSAVIDRAIGYNVQFEPPLP
                     ANEVRHIAKSIAKWTHRNLTPAGFSAVQAARGAKGGKVSKGGGRPSNSGKARADLLPEV
                     LRLKGMGYSNRDIGEDLGISSSTVSLYLKRESE"
                     /name="replication initiation protein"
     gene            complement(2588..2872)
                     /locus_tag="LDN97_RS23720"
                     /old_locus_tag="KAM339_045970"
     CDS             complement(2588..2872)
                     /locus_tag="LDN97_RS23720"
                     /old_locus_tag="KAM339_045970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017411299.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_042650712.1"
                     /translation="MQVKWLRKAALNLEDAHDHLAKENSRVAQEFVLEVYRLVHLLVDQ
                     PAMGRPGRVPGTRELVLQPYPFLIPYRVKGEEIQILRVFHTRLRLPSHW"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2862..3101)
                     /locus_tag="LDN97_RS23725"
                     /old_locus_tag="KAM339_045980"
     CDS             complement(2862..3101)
                     /locus_tag="LDN97_RS23725"
                     /old_locus_tag="KAM339_045980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005740683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_016946643.1"
                     /translation="MSVMSVRLPDEVEQQLGQLAQSTGRTKSWLANQAIQDYLAREAWQ
                     ITEVEAALREADAGDFVPEKEMMAKFNRWGINAG"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(3342..4322)
                     /locus_tag="LDN97_RS23730"
                     /old_locus_tag="KAM339_045990"
     CDS             complement(3342..4322)
                     /locus_tag="LDN97_RS23730"
                     /old_locus_tag="KAM339_045990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416498.2"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="WP_223930743.1"
                     /translation="MSHQLTFADSEFSSKRRQTRKEIFLSRMEQILPWQNMVEVIEPFY
                     PKAGSGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLFARLSLDSALPDRTT
                     IMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQRDPE
                     MHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVSADAG
                     YQGAPQREELAEVDVDWLIAERPGKVKTLKQHPRKNKTAITIEYMKARIRAKVEHPFRI
                     IKRQFGFVKARYKGLQKNDNQLAMLFTLANLFRVDQMIRQWERSH"
                     /name="IS5 family transposase"
     gene            4706..6283
                     /locus_tag="LDN97_RS23735"
                     /old_locus_tag="KAM339_046000"
     CDS             4706..6283
                     /locus_tag="LDN97_RS23735"
                     /old_locus_tag="KAM339_046000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting chemotaxis
                     protein"
                     /protein_id="WP_128274299.1"
                     /translation="MRQNLPVTGRNLELPKDANILSTTSPQSHITYVNPDFIKISGFTE
                     EELLGQPHNIVRHPDMPPAAFEHMWSTLKSGRSWMGLVKNRCKNGDHYWVSAYVTPIAK
                     NGSIVEYQSVRTKPEPEQVLAAEKLYAQLRSGKAARPKLAASFSVKILLLIWGSIISSA
                     MAAGMLTDTSISSLLLATLMSGSLSSVSVLAILSPLGRLVERARNISNNPLSQSLYTGR
                     TDEFGQIEFALRMMQAETGAIVGRIGDASNRLSEHTRGLLKDIESSNVLTVEQQAETDQ
                     IATAVNQMVASIQEVASNAQHAADAAGRADTETASGQRLVAHTSQSITALEGEIRQAAQ
                     VIHELEGQSNEISKVLDVIRGIAEQTNLLALNAAIEAARAGEQGRGFAVVADEVRSLAA
                     RTQQSTTDIQSMISALQERAQSAVTVMEQSSRQADTSVAHAEEAATALDGIGQLVNEIT
                     DMNAQIATAVEQQGAVSEDINRSIINIRDAADTNVQTGQNNLQSAKSVAQLTSALSELA
                     KQFWEKRG"
                     /name="PAS domain-containing methyl-accepting chemotaxis
                     protein"
     gene            complement(6578..6778)
                     /locus_tag="LDN97_RS23740"
                     /old_locus_tag="KAM339_046010"
     CDS             complement(6578..6778)
                     /locus_tag="LDN97_RS23740"
                     /old_locus_tag="KAM339_046010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005321951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_128274297.1"
                     /translation="MSDRFPGLMLWHTVAVMAAVFAAKALLYWAADWLGLGPLYRALAG
                     PSWDVAAWIAALALLLRWMRV"
                     /name="hypothetical protein"
     gene            complement(6786..7073)
                     /locus_tag="LDN97_RS23745"
                     /old_locus_tag="KAM339_046020"
     CDS             complement(6786..7073)
                     /locus_tag="LDN97_RS23745"
                     /old_locus_tag="KAM339_046020"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_128274296.1"
                     /translation="MKSGRLGTPDTVAHIRQFPPVEYELASYQGSPWLWADSEPLIDLL
                     DATIDWMNDDDGSRWIVIDTEYLRQHVAHYALLSADTWRQRLLDAGVEVD"
                     /name="hypothetical protein"
     gene            complement(7173..7514)
                     /locus_tag="LDN97_RS23750"
                     /old_locus_tag="KAM339_046030"
     CDS             complement(7173..7514)
                     /locus_tag="LDN97_RS23750"
                     /old_locus_tag="KAM339_046030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011116720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WP_202979152.1"
                     /translation="MTELERLLTAQFERMEQRHQDETKALRAQLDAQQQSLEGLQTQLQ
                     STLGEYETHCASLHTAFVALEKSTRRELASMGRSLDQTNRATGAALGSLNSSVSIVKSS
                     LESLNKAQS"
                     /name="mobilization protein"
     gene            complement(7515..8216)
                     /locus_tag="LDN97_RS23755"
                     /old_locus_tag="KAM339_046040"
     CDS             complement(7515..8216)
                     /locus_tag="LDN97_RS23755"
                     /old_locus_tag="KAM339_046040"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_202979151.1"
                     /translation="MEKGGELKTRLSERLSQRQAEELAEVATLTEANLTQLSERLTKHV
                     SAELSTTRDAIRQQSEQITTLLGSQQSALEQQQATVDALKRLSLKDLEQIQKGLNDAIT
                     LAQGKAIEQAIDPLRTALEQLKMDGEALSRVTFRGWLKPLLISMAVLLGAGGSTWGVAA
                     YLAKEITGQLTELQNLNQQIGTAQRTLDSLPQGVTFVRDRDGRYIVARQIDTPFKMDEG
                     TYKGLMAVGVK"
                     /name="hypothetical protein"
     Typing          complement(1435..2371)
                     /name="rep_cluster_1195"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.659"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 cggggcaatg ttcagagatt tgcccgtgcc caggtgaaca cggggtacca agatgtggac
       61 gtggacaccg cctcccgctt cccggtgctg cacggcggtc aggtgtacgt cctgcctatc
      121 cagtccggca aaggacagcg cctcgaagtc gtccagcacc gcgccgatct ctgcgtcact
      181 gggggcctct tcctccgacc aggagatcac cgcactccgg tatcgatgct gaaaatcgag
      241 cgcgtccgcc acggcggcga actgctgtgg atcaccccgc agcaccgcca ccccggcccg
      301 ttcctcgccc ttgtggtcgt gactgtccag caaataggtc gccgcactac tgcccttgcc
      361 ggtgccatga gccaggaact taatcatcat gcccgtcacc ctcccggttc ggcggcggcg
      421 gaagctgcgg cagcaacgtt ccggctgcct cctcgatgct gcgtagcaac gccagcagct
      481 ccaccaggta aaccgggctc cccaccaggc ggcattcgtt caccgccctg gcgatctggt
      541 tgaggttcga ccccaacgcc gcgagctggc gcattagcgc cggatctgcc aggtggcggg
      601 ggtctcgttc ccgccgcgac cgggcggggg caatgtgggt ttgctggtcg gcatccacct
      661 tcgctctcag ccagtcggag agggtcaggc cctcgtcaca ggcacccttg cgccacttgg
      721 ctagggtggc aggatcgagg cgaaggctgg ccgttgttgt ctttagctct ggctttgtca
      781 ttggctctcc aagcagcgca gcggcgcagg gtttgaaggg gcggagcccc tcaccagccc
      841 ccgtttacgt tgagccgcga agcgggcgaa aacgtaatac ggggtcggct ggcttaatac
      901 cagtacagca aaaaaacagg gggtcggcaa cttgccaatt atcgtcacag tgacacataa
      961 tcaattaccg ttacagtgac aggaaataac accatgaagg acaccaagga tcaggggact
     1021 ggggacatgt ttggcatcaa gcgcggacgg ggtaggccaa aaaccggatc agccaaaaca
     1081 ggggcagaac gacaagcagc ctaccgtgca aagcagcagg acaataacgt tactgtgacg
     1141 ataaatcggg cattggtaga ggggctggat gcccagatgc aggttatccg ggatggaggg
     1201 tcggcggtgg tactgacccc ggagcaggcc ggggagatcc tgcgggcact gcgaacggca
     1261 gagaacaaac agctccgggg ataagaccta ctgcccacag gccaaacaca tggcctgatg
     1321 gacagccctt cgggatcggc cttacccccc tcgcaccaat cctctctctt gaatgatttt
     1381 aagggctcaa agaaccccct ccgggggcta ctatctgata aggcttgttt cgatcactca
     1441 gattcgcgtt tcaggtacag gctgaccgta ctggacgaga tcccaagatc ctcacctatg
     1501 tcgcggttgc tgtaccccat acccttaagc ctgagcacct ccggtaagag gtctgcacga
     1561 gccttaccgc tattactggg cctgccccca cccttggata ctttcccacc cttggcccct
     1621 ctggcggcct gtacggcgct gaaccctgcg ggggttaggt tacggtgcgt ccacttcgca
     1681 atggacttag cgatgtgcct gacctcgttg gccgggagcg gcggctcgaa ctggacgttg
     1741 tacccgatcg cccgatctat aacggcagag agccaagcct cataccccgg ccacccctgc
     1801 ctaatcgcct tgtaagccca ctggcgcgtt ttctcgaaca aggtgcagtt acgccccaac
     1861 ccgtaagctg gaagctgttt gcggccattg taggggctta aatcgaggta gtcggccagc
     1921 cagctcagat cgtaggaggc gggctcccat acctggacaa gccagtgggt atgcaggggg
     1981 ttcttgcaga tcagcccgga gtagcccaga tccgccccca gtttctcccg gagggcggcc
     2041 tcgatggcgg cggcataccg cagtggggcc accttgccgt ccggggcagt gcggatcggg
     2101 gtctccagtc cgtagatcag gtgggcgtgg ccgttggccc tgttcgtcac cgtgatgttc
     2161 ggtgccgggg cgcctacgtc atcccaggat aacgtagcgg cagcatggtc tacgtcaaac
     2221 acgagccaat gccgatgcgt tggcccgttc ggctggatgt aacgagcccc aagggcacgg
     2281 ctgacatccg caatggacag acccgtggta aattcgtcgg tgtggtaggg cttccggggc
     2341 aggcgctccc ggaacaggtc aagctgtgct cgattcaatg accaccgcgc atattggcgg
     2401 cgctaaaggg ttgtctggtg tgatccatgt gatatatttc acctattgca taagtacggc
     2461 cccgaactct ctgtaatctc ccgccaagtt gatcaacaga agttcggggt ttgtcttttc
     2521 taggcagtac gttaccgcac gaccccctcc atacgcaaac gcataccgcc ctacctacaa
     2581 gcacccttca ccagtggctt ggtagccgca agcgggtatg aaacaccctc agaatttgga
     2641 tctcctcgcc cttcactcgg tatggaatga ggaacggata gggttgcagc acaagctctc
     2701 gtgtaccagg aacgcgccct ggccgtccca ttgctggctg atctaccaac aagtgaacca
     2761 agcgatacac ctctaataca aactcctggg ccaccctgga gttctctttt gctaagtgat
     2821 cgtgagcatc ctcaaggttc agtgcagcct tacgcagcca tttaacctgc attgataccc
     2881 caccgattga acttcgccat catctccttt tcaggcacaa agtcgcccgc atccgcttca
     2941 cgcaatgcgg cttcaacttc cgtgatctgc caggcttcac gcgccaaata atcctggata
     3001 gcctgattcg ccaaccacga tttggttctt cctgttgatt gggccagttg ccccaactgt
     3061 tgctctacct catccggtaa gcgaacggac ataacactca tatacacctc acaaaaccac
     3121 gatgtttaca tgatactaca ccttgactga atgacaggca cggacaacaa cccagagtct
     3181 cgttgtccga cctggggaag gtgcgaataa gcggggaaat tcttctcggc tgactcagtc
     3241 atttcatttc ttcatgtttg agccgatttt ttctcccgta aatgccttga atcagcctat
     3301 ttagaccgtt tcttcgccat tcctggcgtt atttccggtt tttagtgaga tctctcccac
     3361 tgacgtatca tctggtccac ccgaaacagg ttggccaggg tgaataacat cgccagttgg
     3421 ttatcgtttt tctgtagccc cttgtatctg gctttcacga agccgaactg ccgcttgatg
     3481 atgcgaaacg ggtgctccac tttggcccgg atgcgggctt tcatgtattc gatggtgatg
     3541 gccgttttgt tcttgcgtgg atgctgtttc aaggttttta ccttgccggg acgctcggcg
     3601 atcagccagt ccacatccac ctcggccagc tcctcgcgct gtggcgctcc ttggtagccg
     3661 gcatcggctg agacaaattg ctcctctcca tgcagcagat tacccagctg attgaggtca
     3721 tgctcgttgg ccgcggtggt gactaggctg tgggtcaggc cactcttggc atcgacacca
     3781 atgtgggcct tcatgccaaa gtgccactga ttgcctttct tggtctgatg catctccgga
     3841 tcgcgttgct gctctttgtt cttggtcgag ctgggtgcct caatgatggt ggcatcgacc
     3901 aaggtgcctt gagtcatcat gacgcctgct tcggccagcc agcgattgat ggtcttgaac
     3961 aattggcggg ccagttgatg ctgctccagc aggtggcgga aattcatgat ggtggtgcgg
     4021 tccggcaagg cgctatccag ggataaccgg gcaaacagac gcatggaggc gatttcgtac
     4081 agagcatctt ccatcgcgcc atcgctcagg ttgtaccaat gctgcatgca gtgaatgcgt
     4141 agcatggttt ccagcggata aggtcgccgg ccactaccag ccttggggta aaacggctcg
     4201 atgacttcca ccatgttttg ccatggcaga atctgctcca tgcgggacaa gaaaatctct
     4261 tttctggtct gacggcgctt actgctgaat tcactgtcgg cgaaggtaag ttgatgactc
     4321 atgatgaacc ctgttccatg gctccagatg acaaacatga tctcatatca gggacttgtt
     4381 cgcaccttcc ttagggtata gcctagattg acatccgcga tgcaaccctt aacttgcttg
     4441 cacctatcgt ttccatgcta aatagctgtt ccgaatagta gatcactgta ctcgtaccgc
     4501 ttctacaggt gacatggagc acacatcgtc gctgagcctt gtcacttcag gcgttgccgt
     4561 cagattgcac aaattcctta cgccacaagg cttccggcaa gatgtagcac tattaggatc
     4621 agttatttag ctttatcgta acgctcaaga aattgggctt aatgccgaaa acaataaaat
     4681 aaaacagcca acccttgagg ttcttatgcg ccagaattta ccagtgacgg gtcgaaactt
     4741 agaactccca aaagatgcca atattctttc gactacctcc cctcaaagcc atatcacgta
     4801 cgttaatcct gacttcatta aaatcagtgg tttcactgag gaagaactat taggccagcc
     4861 tcacaacatc gtaagacacc cagatatgcc gcctgctgca tttgagcata tgtggagtac
     4921 attaaaatct ggccgctcat ggatggggct agtaaaaaat cgctgtaaaa atggcgacca
     4981 ctattgggta agtgcttatg taacgccaat agctaagaat ggttcgattg ttgaatatca
     5041 gtctgtaagg accaagcctg aacctgagca ggttttggct gcggaaaaat tatatgctca
     5101 attgagaagc gggaaggccg cgaggccgaa attggctgct agcttttccg tgaaaatact
     5161 cttgctcata tggggtagta ttatatcaag cgcaatggct gccggcatgc ttactgatac
     5221 atcaataagc agcttattgt tagccacttt aatgtcagga agcttaagct ctgttagtgt
     5281 tttggctatt ctctctcctc ttggaagact ggttgaaaga gccaggaata tttccaataa
     5341 cccattaagt caatccctct acactgggcg caccgatgag tttggccaaa tagagtttgc
     5401 tttacgaatg atgcaagctg aaacaggcgc catagtaggt cgcataggtg atgcatcaaa
     5461 tcggcttagc gaacacaccc gaggcctact aaaggatatt gagtcaagca atgtacttac
     5521 agttgagcag caggcagaga cagatcaaat agcaacggca gtaaaccaaa tggtggcaag
     5581 cattcaagag gttgcgagca atgcacagca tgctgcagat gcggccggaa gagcagacac
     5641 tgagacggca tctggccagc gtctggtagc ccacacaagc cagtcaatca ctgcccttga
     5701 aggtgaaatt aggcaagccg ctcaggttat tcatgagctt gaaggtcaaa gtaacgagat
     5761 atcaaaagtt cttgacgtta tacgagggat cgccgagcaa acgaatttgt tggcactcaa
     5821 cgcagcaatt gaggccgcgc gtgctggtga gcaggggcgt ggttttgctg ttgtcgccga
     5881 tgaggttcgc agtcttgctg ctcgcacaca gcaatcgaca acggatattc aaagcatgat
     5941 cagcgctcta caagagcgag cgcaatccgc tgttacagtc atggagcaaa gtagtcggca
     6001 agcggacacg agtgtagctc acgcagagga agcagctaca gctcttgatg gaattggcca
     6061 actcgttaac gaaattaccg acatgaacgc gcaaatagcg actgcggtcg agcagcaggg
     6121 agcagtaagt gaagacataa accgcagtat tatcaatata cgcgatgctg ctgataccaa
     6181 tgtacagacc gggcagaata atttgcaaag tgcgaaatct gtcgctcagt taactagcgc
     6241 tctgagcgaa ctggcaaaac agttttggga aaaacgagga taacgctttt cagtatctcg
     6301 acagggataa cttactttac catcggtatc ccttttagcc gcacaaattt tagccatggc
     6361 ttttcaggaa gtcagggcta tcagaatggc cttagaaagc ctagtcaaag agctgtcacg
     6421 agaacaccgt tagcttagcg tacgattttt tccgaattct gcggttcccc cggatcctgc
     6481 agggtgtttc tggatcaggc cacactcccg aacggggacg actagcgcag tctgtcgtgc
     6541 ccccttcggg agtcgccaag cgcagcgcgg caggcagtta gacccgcatc cagcgcagca
     6601 gcagggccag cgcagcaatc catgccgcca cgtcccagga aggccctgcc aaggcgcggt
     6661 agagcggacc aaggcccaac cagtccgcag cccagtacag cagggctttc gccgcaaaca
     6721 cggcggccat taccgccacc gtatgccaca acatcagccc cggaaaccga tcactcaatc
     6781 gcacctcaat ccacctccac gcctgcatcg agcaaccgtt gccgccatgt atcggcggac
     6841 aacagggcat agtgggccac atgctggcgc aggtattcag tatcaatgac gatccagcgg
     6901 ctgccgtcgt catcgttcat ccagtcgatg gtcgcatcga ggaggtcgat cagcggttca
     6961 ctatccgccc atagccatgg ggagccttga tagctcgcca gttcatactc aaccgggggg
     7021 aactggcgaa tatgcgccac cgtgtcagga gtacccaacc gacctgattt catacccgat
     7081 ctccttaatt attgtcactg tgacattata ttacatgctc aacccgctac tctggctctg
     7141 ctcttttcgc agcaccgcgg ccctatcgac gatcaactct gcgccttgtt caagctctcc
     7201 agcgagcttt tgactatgct cacactgctg ttcaatgacc caagtgctgc tccggtagcc
     7261 cggttggtct ggtccaaact ccggcccatg ctggctagct cgcgcctggt gctcttctcc
     7321 aaggccacga aggcggtgtg taagctggcg cagtgcgtct cgtactcgcc caatgtgctc
     7381 tgcaactgcg tctgtaagcc ctccaaagat tgctgttgcg cgtcgagctg cgcccgcaag
     7441 gctttcgtct cgtcctggtg ccgttgttcc atccgctcga attgagctgt cagcagccgc
     7501 tctaactcgg tcatctactt cacccccacg gccatcaggc ccttgtacgt cccttcgtcc
     7561 atcttgaacg gtgtatcgat ctgcctcgcc acgatgtacc gcccatctcg gtcgcgcacg
     7621 aacgtcaccc cttggggcaa gctgtccagg gtccgttggg cagtgccgat ctgctggttt
     7681 aggttctgaa gctcggtcag ttgcccggtg atctccttcg ccaggtacgc tgccaccccc
     7741 caggtactgc ccccggctcc caggagcacc gccatcgata taagcagcgg tttgagccat
     7801 ccccggaacg tcacgcgact cagggcctcc ccgtccatct tgagttgctc tagggccgtc
     7861 ctgagcggat cgatcgcctg ctcgattgcc ttcccctggg cgagggtgat cgcgtcgttt
     7921 aaccccttct ggatctgctc caagtccttc aagctcaagc gtttgagcgc gtccacggtc
     7981 gcctgttgtt gttccaaggc gctctgctgt gaacctaaca acgtggtgat ctgctcgctc
     8041 tgctgccgaa tagcgtccct ggttgtactc agctcggcgc tcacatgctt cgtcaaacgc
     8101 tcgctgagct gtgtcagatt ggcctcggtc aacgtggcca cctcggccag ctcctcggct
     8161 tgccgctgcg atagtctctc gctcaagcgt gttttcagct cgccaccctt ctccatacag
     8221 ctctcctttg agccggatgc cctgcgggta tccttccggc ctgatcgtga tgtagtcctt
     8281 gccctggcgg gtgatctcga attcgagcac ctcgcccagg tactcgagta attcgccccg
     8341 gtcgcggatc agcccctgag ccaccgcccc aagcaggtga ccccctatct gccgcttgat
     8401 ggcagtcctg tccttctggc ccttgtaggc gtcaaaggag ggctgtatta gccgtgcccg
     8461 gagcgggtcg tctggtctgg cccaaccctt gccgtagttc catttatctc gcagggcatc
     8521 aaagtccttt cgatgtccgg g
//
