LOCUS       NZ_AP024841             6303 bp    DNA     circular CON 04-NOV-2023
DEFINITION  Enterococcus faecium strain AA622 plasmid pAA622-4, complete
            sequence.
ACCESSION   NZ_AP024841
VERSION     NZ_AP024841.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00324536
            Sequence Read Archive: DRR305792
            Assembly: GCF_019977575.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Hashimoto,Y. and Tomita,H.
  TITLE     olecular characterization of the VanD-type vancomycin resistant
            Enterococcus faecium clinical isolates from a patient after
            vancomycin therapy
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6303)
  AUTHORS   Hashimoto,Y. and Tomita,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUL-2021) Contact:Yusuke Hashimoto Gunma University
            Graduate School of Medicine, Bacteriology; showa 3-39-22, Maebashi,
            Gunma 371-0034, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: canu v. 2.1.1
            Genome Coverage                   :: 80x
            Sequencing Technology             :: Illumina Miseq; Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/04/2023 02:43:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,289
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 156 of 238
            Pseudo Genes (incomplete)         :: 82 of 238
            Pseudo Genes (internal stop)      :: 44 of 238
            Pseudo Genes (multiple problems)  :: 37 of 238
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP024841.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="AA622"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pAA622-4"
                     /geo_loc_name="Japan"
                     /collection_date="2014"
     gene            629..715
                     /locus_tag="LDN47_RS16255"
     CDS             629..715
                     /locus_tag="LDN47_RS16255"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_107532029.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(836..1525)
                     /locus_tag="LDN47_RS16260"
                     /old_locus_tag="GVanDAA622_33310"
     CDS             complement(836..1525)
                     /locus_tag="LDN47_RS16260"
                     /old_locus_tag="GVanDAA622_33310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002321377.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLVG
                     VIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTACY
                     EDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQTEEA
                     CCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLESVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(1770..2684)
                     /locus_tag="LDN47_RS16265"
                     /old_locus_tag="GVanDAA622_33330"
     CDS             complement(1770..2684)
                     /locus_tag="LDN47_RS16265"
                     /old_locus_tag="GVanDAA622_33330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002321380.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRT
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKLKEK
                     NEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKKKVRS
                     NKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTPEQSEI
                     RPTRRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2666..3046)
                     /locus_tag="LDN47_RS16270"
                     /old_locus_tag="GVanDAA622_33340"
     CDS             complement(2666..3046)
                     /locus_tag="LDN47_RS16270"
                     /old_locus_tag="GVanDAA622_33340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_002321381.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPDDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(3368..3571)
                     /locus_tag="LDN47_RS16275"
                     /old_locus_tag="GVanDAA622_33350"
     CDS             complement(3368..3571)
                     /locus_tag="LDN47_RS16275"
                     /old_locus_tag="GVanDAA622_33350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002321382.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQL
                     YKSGLFLVLILVVLDIIKSKKK"
                     /name="hypothetical protein"
     gene            complement(3583..4116)
                     /locus_tag="LDN47_RS16280"
                     /old_locus_tag="GVanDAA622_33360"
     CDS             complement(3583..4116)
                     /locus_tag="LDN47_RS16280"
                     /old_locus_tag="GVanDAA622_33360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002334266.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENVV
                     IEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAINAA
                     FWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQRGY
                     DAAGRVVATRYSTL"
                     /name="hypothetical protein"
     gene            complement(4566..4736)
                     /locus_tag="LDN47_RS16285"
                     /old_locus_tag="GVanDAA622_33370"
     CDS             complement(4566..4736)
                     /locus_tag="LDN47_RS16285"
                     /old_locus_tag="GVanDAA622_33370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002292472.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(4752..5285)
                     /locus_tag="LDN47_RS16290"
                     /old_locus_tag="GVanDAA622_33380"
     CDS             complement(4752..5285)
                     /locus_tag="LDN47_RS16290"
                     /old_locus_tag="GVanDAA622_33380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002321384.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLREQL
                     ALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATETID
                     KQIDTEKKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            complement(5306..6256)
                     /locus_tag="LDN47_RS16295"
                     /old_locus_tag="GVanDAA622_33390"
     CDS             complement(5306..6256)
                     /locus_tag="LDN47_RS16295"
                     /old_locus_tag="GVanDAA622_33390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_033642128.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRL
                     TFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIRGT
                     AKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFAYFS
                     KEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVVGYQF
                     TFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQI
                     KQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
                     /name="replication initiation protein"
     Typing          complement(5307..6255)
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.953"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(1774..2684)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.079"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gttttgtctg tgataatgca tattataaca tacgtgagtg caaaaaaaga gtttaaaagc
       61 agacaaaatg tagtttaaaa gcagacaaaa tgtagtttaa aagcagacaa aatgtagttt
      121 aaaagcagac aaaatgtagt ttataaaacg ctgtatccat tgaaggagta aggacacagc
      181 cgacggtaaa gaggtttaaa aaaaggttta aaaagagatt ataaaagagg gtgacaaaat
      241 ttcccgccta aaaaccactt cttttttcta ccaacaggtt cgcgcatttt tcaaaactgc
      301 caattcaaaa ccttgctccc tacggtcgct aaaaaagtgt gatcgctatg ctctcacatt
      361 gtactgcgca gtcgcctaaa ggctcctttg ctaaaaacaa gtcagtaacg gaggtggggg
      421 aagcctctga tagctcgtct aagtcgtttt aagacagtct tgggtaaaaa accttatctt
      481 gttcttaaag cgccttacag gcggtcaggg caagccctaa caacccgaaa ataagggata
      541 cttaaaaaca ccacacagat tttattttgt aaggtataat aacttgtcgc ctatccgaca
      601 gtcttgcgac tggaaggagg tgtatctaat gtttgaacgg ttcatttgcc cccttcttgt
      661 agaagtttgt gtctctttat tcagttattg gttgaataac cgaaacaaga agtaaaatca
      721 ataaaggcga catcaacctg aaaaaaagcc ccagagaatc cgacctctct ggggctttcg
      781 tgtatctaat gtttgatcat ttgcaggttc agtttaacat gattgctaat caaattcaat
      841 tttgttgttg ctgatccaac cattccttga cactttctag ataatttaat gccgtttctt
      901 ttttcgttgg attttgtatg tttgttgctt gctgaagcag tgtttcataa cgttcgtatg
      961 tgaccgattg ttgccgttct tttcgttctt gttcactggt tgaaaaaggg attgtatcgc
     1021 taaataagtt acaacaagct tcttctgtct gcgtatcttt aaaatattgc acgtcttttt
     1081 ttacttgctc atatttttct tgtacctctt tttgctgttt ttctggtaag ttttgataaa
     1141 aacgagtgag ttttttcatg tttttatctg ttaaacgttt ctcttgatcc acaaaaatat
     1201 tgttcatgtc ctcataacaa gcggtaaatt cctccatttt ttgtgctaat gcttgctctt
     1261 ttttcttttt atcacatgct gcttcatact tttgagtgat atcccctaat aaaatgcttc
     1321 ttgaaacacg tttattaccc tctaaaacgc cgaataaacc acttaaaacg attgataagg
     1381 caaatataac cccgaccaat aaaaatcgct taaatcgaat tttagagcct tttaatgaat
     1441 atcttgtttg ataagcctta tagctaagcc aaataattcc taaaatactg gcagaaatga
     1501 gtagataggg taaatagaat gccatgtctg tttattctcc ccataccaac caaaaggcta
     1561 ataatgttag tactaagcaa cataaggggg caagaatatc catcgatcgt tgaattaaag
     1621 cctttttttg ttcttggttt cgctggtcta atagcgcttg gtggagtttt ttgacttgtg
     1681 aatccatcgt agtttgtatc ctttcttggg tataaggcgc aagaaaggca cgttgtctct
     1741 ttatttcgtt ttcgatgtct tttaacgttt tagagcgata attcttgttg gtggtctgtt
     1801 cctcgttcaa cgtgttgttc atggtttctt cgagtcggtc taatttccga ttgttcaggc
     1861 gtaaaagttc gttgttctct tcgtacttct tctgcagttt cgcgaatgct agctcgagct
     1921 cttcgtagct cgttggttcg ttgttgattg taggttcgtt ctcgtctgtc cagctcatgt
     1981 aaaatcgtct ccttttcgta atcctttcct agtttgttgg atcgtacttt tttctttttg
     2041 tggaccaatt cgtaagtaaa attttttccg cgattcaagc aatgaatgcc gaatgtttta
     2101 agtgaatcac aaaattgggt atacgttcga gtttttttgt ttttcattgt ttgatcaatc
     2161 gcaaaccgaa tttcgtcttt ccaaaccggt tcattttttt cttttaattt aatttcagac
     2221 atggttcgtt tttcggtttt ctttcctcgt tccgtgattg ttaagtcatg ttcaagacaa
     2281 atctcgtcat ttaggtcata acatttgttt aaaaatgctt gcccatgggc ttgaaatttt
     2341 ttcccagttt caagattaac ggagttaatg acaatgtgat tatgaatatg gtctttgtct
     2401 gtatgtgtgt aaatcgcgat ttggtggtta ggggctattt ttttcgcata ctctgcccct
     2461 agttgattgg ctttttcagc ggtaatttct cctggtcgaa aggactgaat caataagtgt
     2521 gcttgtacac gatcgtcttt tccataaagc gtgcgtattt gtttcatttc acttttggca
     2581 taatcaatgt cacagtttat tcctgttttg acggttgctc gtggttctgc gtaattgatt
     2641 gctcggctac atgaagtggt tcgacttact ttaactgttg ccatatcttt tgtagctcct
     2701 tttgcatgat ttttaggttt tcggataaac gttgagcatc atcaggagca acgtcgtgtt
     2761 ggtgtgtatt gcaccacttg gcaatttgat tgatattccc tccagctttg gctaaatcgc
     2821 gcgcgattgc ctgggcaatg tctggcgcca ctttcggtgt caccagacgc gcgttttgtg
     2881 ccttggcttt cacgaaagcc ggcacactca tttgcaaagc ttcagctgac tgcttcagct
     2941 ttgcaaattc ggattcgctc actcgaaaac tgatttgttt tggctcggat cgataggtat
     3001 attttttaga atgatcgcta gccaaatttt gatgttgttc gctcataagt cccacccttt
     3061 cagtacgtcc tacggacgta tcgttcgcaa taccattgta cctaaaaagg tacaatgagt
     3121 aaagctcact aaaagggttt ggcaagctct atatttgcta gccaaatacc tccgtatttg
     3181 gtgcaaataa ccgcttgtca aaggagcgca agctccccct tgaatccccc tcaaaatcca
     3241 cgctccacaa tacaaaaaaa ggctagcatt tgctagccac tttttgtaaa gtttcgctta
     3301 tttcaccgaa ggtgaaattg ttgctttgta atattcaaac aaccctattc ttctaaaatg
     3361 tatcgattta ttttttttta gactttatta tgtctaagac aactaagatt aacactaaaa
     3421 ataaaccaga tttgtataat tgccctccgc ttagcatatt aacaattata gcaacagcta
     3481 tagcaaatat aaaaatacga atagtagcta tttgcggatt ctttttagta aatgttttta
     3541 atgattttaa tgatttagat atcaaaaaca tcactcctta acttataacg ttgaatatct
     3601 agtagctaca actcttccag ctgcatcata tcccctttgg taataaaatc caatccagcc
     3661 attaccattt ttgtcccatt cttttgcaac tgcattccct ggagaagcac cttttttagt
     3721 ccaacttgca cctgttaaca aagcaccaat tgcccaacct gggataccaa acatagaagt
     3781 gctagctttc cctcctgccc aaaaggcagc attaatagca ttagcagcta ctccagtggt
     3841 tacagcaata tttgtatatt cctttttgcc ccatcttgca actgcagctc ttgtcatgat
     3901 atctgaagaa tttgcaaaat cttccgcact ataaccatcc tcatatttag ttacgaccat
     3961 accatcttca gtgtactcaa taactacatt ttcagtagca ttgttttcgt ttaatgatac
     4021 cacttgggaa ttttctgaag cataaactat agtagttgac ggtaaaacaa ctgccccaat
     4081 agttaacaaa gctaccccta aagtaaattt tttcatagaa tttcctcctg atacagaaaa
     4141 atatatagaa aacgttttct ttgtgaaaat cataaaacaa aaggtatagg cttgtcaata
     4201 ttttatttag taaagataat ttatattttt gataatgtgg tatagttaga atgcattagg
     4261 aaaatatgtt atattaaatt cttgttgaca taatgtacac tatgccaagt agcccttgta
     4321 tcaaccgata cagaggctca ctttgtgatt ttcgattggt ctaggtcaaa agctcttttt
     4381 aagcttgatt ttctgcggtt gcttctttcc gtttctttgt gaatttttac cagtcatttt
     4441 ttactgggtt gttattttta ataaaatatg ataaattttt ggtagcgctt cataagcgcg
     4501 ttgctttttc aagaataaac actcattatt ttttaactgg ctgctcgaaa gagcagtctt
     4561 ttttattatt tatctgtttc ttttacaaac aattcttcgt taggactgtt gtaataattt
     4621 ggattcaatt tcaattcacc atgtttattc cctttattga tcactgacaa taacatcgga
     4681 ctgaatccta gaattcctag taaaatagaa ccgaatagaa gattttttct tgtcatgaat
     4741 gttccctcca gttatttcca aagttgccac catttttttt cagtatcaat ttgtttgtct
     4801 attgtttctg ttgcttcaga aagagtcgtt gagtcattct ctggagcttc ttctgtataa
     4861 gtcaactgta gtttttcttg cagtttttcg atttgtttgt tggcttgtag ggtgagttgt
     4921 tgttgctggt cgagtagttt ttgtatatga gaattatttt gctctctgag ggcaagttgt
     4981 tctcgtaaaa attcattttc ttcacgcaac tgatctagca acttgtttgt aaccacgttt
     5041 ttgcttggta tattaacatt tttatcgttg tcttgttcgt actcgtgcaa cctatctagc
     5101 aacatttggt tgcctaagtt gcgagcaagt tgcaatactc cagaacgcaa tacaacctta
     5161 taaccacgct catttttagc tatcatggaa gaatctaatt tttggatatg tttcgatatt
     5221 gcttgcctac tcatcttaaa aagttctgac aattccttta ttgtaaaggt ttcttgtggt
     5281 tgcatactgg ttgcctccta ggttgctact ggaataaatt atctaaatca gataataaac
     5341 tttttttctc ttttttcgaa agtgcggact ctgtttgttt ttcaagagct tctttctctt
     5401 tttctagttg tttgatttgt tggaggagtt caagttctcg tttcttctgt ttgatttttt
     5461 cgttaattgt tgtagcaggt tcttcgtatt ctttttgttt atcagctaaa gccaatgctt
     5521 tattttgttc ttctttggtt ggtgcatgtt tattaaaatc atctgctttt ttgatttctg
     5581 gtttaaacgt aaattgatag ccaacgacag gttttcctcg cccttttccg tatttctttt
     5641 tgattgataa tcctttgaag attgcggtta attcctgtcg tataggcttt ataacacgat
     5701 tatcaacgtt tgcttggttg ttccagtaac tttttggaat atctaatagt tcatgaaaat
     5761 cctctttaga aaaataagca aaacctttag ttcgatattg ttttagtaat cgaaacatag
     5821 tctttgcata gctactttgt aattcgttaa attgttggag tgaatagcga acccactcat
     5881 ctaaattgtt tagtaaaggt aaagcttttt cgtaaacttg tatgtctaca tatggatctt
     5941 tagcagttcc acgtatttta aactcactaa acataacgaa tctatctata tcgagcccat
     6001 tagcacttct tttaccaaaa cgtaaatcca ttaatttatc ataagtcatt tctaaatcat
     6061 taatgaaacg attattagca gttgctttat aattacttaa atcttttagt tggtcaaaag
     6121 taagacggac tgttttatcg cctttttcgc gcattctaga cacaatagag aaaaatagat
     6181 tcatttcaac tggtgtaaat ttccgcaacg gaatggtatt taattcatga tggtatttca
     6241 cgatttcatt tgccaaagat attcctcctt actccttcaa tggatacaga cgttataaac
     6301 aac
//
