LOCUS       NZ_AP024840             9507 bp    DNA     circular CON 04-NOV-2023
DEFINITION  Enterococcus faecium strain AA622 plasmid pAA622-3, complete
            sequence.
ACCESSION   NZ_AP024840
VERSION     NZ_AP024840.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00324536
            Sequence Read Archive: DRR305792
            Assembly: GCF_019977575.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Hashimoto,Y. and Tomita,H.
  TITLE     olecular characterization of the VanD-type vancomycin resistant
            Enterococcus faecium clinical isolates from a patient after
            vancomycin therapy
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9507)
  AUTHORS   Hashimoto,Y. and Tomita,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUL-2021) Contact:Yusuke Hashimoto Gunma University
            Graduate School of Medicine, Bacteriology; showa 3-39-22, Maebashi,
            Gunma 371-0034, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: canu v. 2.1.1
            Genome Coverage                   :: 80x
            Sequencing Technology             :: Illumina Miseq; Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/04/2023 02:43:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,289
            CDSs (total)                      :: 3,198
            Genes (coding)                    :: 2,960
            CDSs (with protein)               :: 2,960
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 156 of 238
            Pseudo Genes (incomplete)         :: 82 of 238
            Pseudo Genes (internal stop)      :: 44 of 238
            Pseudo Genes (multiple problems)  :: 37 of 238
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP024840.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="AA622"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pAA622-3"
                     /geo_loc_name="Japan"
                     /collection_date="2014"
     gene            complement(join(9494..9507,1..1960))
                     /locus_tag="LDN47_RS16195"
     CDS             complement(join(9494..9507,1..1960))
                     /locus_tag="LDN47_RS16195"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_223924341.1"
                     /translation="MLKKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQY
                     KDDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILARN
                     DSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLKKYG
                     TPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNSFDTI
                     KDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIVTLSIY
                     HLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINIHNEAENNSLKYWERTT
                     NLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQENNKEFFI
                     GQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTTYILVPKKY
                     KEYAKMIEKNYLEDIQFRLEDSPPQAPYKTPNLKNLKIKLIFVNNNQKYFSFNTLYGKA
                     KDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQINPYIKEFNL
                     ENIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIFVSFTINSKKIA
                     LKKIFGYSPISQVLDLCLLPLQ"
                     /name="hypothetical protein"
     gene            complement(2014..2388)
                     /locus_tag="LDN47_RS16200"
                     /old_locus_tag="GVanDAA622_33190"
     CDS             complement(2014..2388)
                     /locus_tag="LDN47_RS16200"
                     /old_locus_tag="GVanDAA622_33190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_000713874.1"
                     /translation="MKKFLCLSALMGVLLTSGGIVSATEALNLDVPEDHTAIYGGGMEA
                     TEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKAGV
                     RAYATCNATLTGNTGWWNVY"
                     /name="lactococcin 972 family bacteriocin"
     gene            2521..2982
                     /locus_tag="LDN47_RS16205"
                     /old_locus_tag="GVanDAA622_33200"
     CDS             2521..2982
                     /locus_tag="LDN47_RS16205"
                     /old_locus_tag="GVanDAA622_33200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000389746.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPDI
                     ETLKKICEFYSTNINSILNEDNLKNKQNNLLVLTAIIFNIFVTILGLFELIQMNFFSFT
                     HGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTIFFPLVILIILLS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3296..3466)
                     /locus_tag="LDN47_RS16210"
                     /old_locus_tag="GVanDAA622_33210"
     CDS             complement(3296..3466)
                     /locus_tag="LDN47_RS16210"
                     /old_locus_tag="GVanDAA622_33210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002292472.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(3482..4018)
                     /locus_tag="LDN47_RS16215"
                     /old_locus_tag="GVanDAA622_33220"
     CDS             complement(3482..4018)
                     /locus_tag="LDN47_RS16215"
                     /old_locus_tag="GVanDAA622_33220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002303560.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLASNLGNQRLLDRLHEYEKDNDKNIYISSKNVVVNELLDKLREENEFLREQL
                     SLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLVYTEETPEDESTTILSEETESI
                     EKHTKKKQKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            complement(4039..4983)
                     /locus_tag="LDN47_RS16220"
                     /old_locus_tag="GVanDAA622_33230"
     CDS             complement(4039..4983)
                     /locus_tag="LDN47_RS16220"
                     /old_locus_tag="GVanDAA622_33230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002303559.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRF
                     TFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKINGD
                     VENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFAYFS
                     KEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGYQFTF
                     KPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQIKQ
                     LEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
                     /name="replication initiation protein"
     gene            5620..5706
                     /locus_tag="LDN47_RS16225"
     CDS             5620..5706
                     /locus_tag="LDN47_RS16225"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_106914087.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(5827..6516)
                     /locus_tag="LDN47_RS16230"
                     /old_locus_tag="GVanDAA622_33240"
     CDS             complement(5827..6516)
                     /locus_tag="LDN47_RS16230"
                     /old_locus_tag="GVanDAA622_33240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002303565.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFLLG
                     CIFSLIIVSSGLLGVLEGNKRVSRSILLGNVTQKYESARNKKKKEQALAQKIEKFTACY
                     EDMNDIFVKQEKRLTDKNMETLTRLYRNLPEEPQKEYQEKYEQVKKDVQYVKDTKIEEA
                     CSDLFGDTNPWFASEEEKKEKQQSVTYERYENLFQQATNIQSPTKKETALNYLESVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(6518..6796)
                     /locus_tag="LDN47_RS16605"
                     /old_locus_tag="GVanDAA622_33250"
     CDS             complement(6518..6796)
                     /locus_tag="LDN47_RS16605"
                     /old_locus_tag="GVanDAA622_33250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_033627308.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLAPYTQERIQTTIDLQ
                     VKKLHQELLDQRNKEQKKAFIQRSMDFLAPLCCLVLTLFAFWLVWGG"
                     /name="hypothetical protein"
     gene            complement(6756..7670)
                     /locus_tag="LDN47_RS16235"
                     /old_locus_tag="GVanDAA622_33260"
     CDS             complement(6756..7670)
                     /locus_tag="LDN47_RS16235"
                     /old_locus_tag="GVanDAA622_33260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002303564.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGKEYAEKIAPEYQIAIYTHTDKEHIHNHI
                     VINSVNLETGKKFQAHGQAFLDKCYDINDEICLTHGLSITERGKKEEKRTMAEIKIKEK
                     NEPVWKDEIRFAIDQTMKNKKTRTYDQFCDSLKTFGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(7652..8032)
                     /locus_tag="LDN47_RS16240"
                     /old_locus_tag="GVanDAA622_33270"
     CDS             complement(7652..8032)
                     /locus_tag="LDN47_RS16240"
                     /old_locus_tag="GVanDAA622_33270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_002292464.1"
                     /translation="MSEQNQNLASDPSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPGDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(8474..8611)
                     /locus_tag="LDN47_RS16245"
                     /old_locus_tag="GVanDAA622_33290"
     CDS             complement(8474..8611)
                     /locus_tag="LDN47_RS16245"
                     /old_locus_tag="GVanDAA622_33290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002303562.1"
                     /translation="MILLKKEYEKFYDRLIYSLQLPKKLNRQLQKTYETKSTEEWESYC
                     "
                     /name="hypothetical protein"
     gene            complement(8725..9345)
                     /locus_tag="LDN47_RS16250"
                     /old_locus_tag="GVanDAA622_33300"
     CDS             complement(8725..9345)
                     /locus_tag="LDN47_RS16250"
                     /old_locus_tag="GVanDAA622_33300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_002303561.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTLL
                     NNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAKKF
                     NSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEPTGN
                     LDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
                     /name="ABC transporter ATP-binding protein"
     BGC             1..7388
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(4040..4983)
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(6760..7670)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.684"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtaaacataa atctaaaact tgacttattg gtgaatatcc aaatattttt tttaaagcta
       61 ttttttttga atttatagta aaactaacaa aaatcatttg aaaattaatt attataaata
      121 atcctaatat taaaagatat tgtagatatg catttaattt atttactttt ttttgagata
      181 ataattcacc agtttcttga aaaacagaat aagaaaaatt tatgatattt tctaggttaa
      241 attcttttat atacggatta atttgattaa aaaagttttg gtttgaagta tgtttctgat
      301 caaaaaataa tccaccatca ctagtcaata tatttcccca aaataatgat tctgttattt
      361 ctggattaat aactctaact atgggatcag ttattgtata gttatctttt gcctttccat
      421 ataaggtatt aaaactaaaa tacttttggt tattattaac aaatattagt tttattttta
      481 aatttttcaa attaggagtt ttataaggag cttgaggagg agagtcttca agtctaaact
      541 gtatgtcttc taaataattt ttttcaatca tttttgcata ttctttatat tttttaggta
      601 ctagaatgta agtagttagt ggatcagagt ttattaaatt taagtcagta gattcttcaa
      661 aagaaatagg attgtaatat tttagatagt ttaaatcaac acatattcct gcaccagcag
      721 gttgactaac atagtcttca aaatttggat aaactttttg tccaataaaa aattctttat
      781 tattttcttg gatagttgcg taattgtatg gcgcgataat aaaagtttta taattcttct
      841 gtattttata aacaaattta ctagcttttt tcaagtagtt attttcttct acggtgttgt
      901 ttctatttaa ttgattagta atatttgttt tatataaatt agtagttctt tcccaatatt
      961 taagagaatt attttcggct tcattatgga tatttattac ttttgtagaa gctattaaca
     1021 gaaggatcat gcttaagaat agacagatat ttaatataca aagatttaac ttaagtggtc
     1081 gtgaattttt tagataattt tttatactag ataaatgata gatacttaga gtaactatga
     1141 taaataaaaa actaattata aaatgaaaaa tactacatag aagaaatgat aggaaaaaat
     1201 tttcgttact gtaatatatg ctcaaaaaaa tagataaaat gaaagttata atcaaagaac
     1261 aaaataagag gtttaactct ttaatagaat cttttatagt atcaaaagag ttatagccta
     1321 acattttttt tagagtgatt tctttcattt ttgaaaaagc aaatataatt gtagtaatta
     1381 caataaatat actaagatag cagataacaa gaacaatatt agtattaatt tgaaaagtat
     1441 ctgattgatt gatattggaa atagtaggtg ttccatattt tttcagtaca ggaattaatt
     1501 tgtttgagtt tccttgtaca tataatattt cacttaatcc aatattttcc acatgagaaa
     1561 aaggataaat atttatattt aaaaaattat ttggaagttt taattggtac ttagaatttt
     1621 tgttattagt ataatggact ttagaatcgt ttcttgctaa tatatttttt ttagaaaatt
     1681 tccagtgttt gttagcgtta gggtttgtag caagaatagt tagttggtta acagcagtaa
     1741 aactatattg agaaatatta atattttctt gtgtagatat ttcatacaaa gcgtttatat
     1801 aactgttaaa gtccatatca tctttatatt gcatatgaat ttcagcggta tttttatata
     1861 gcgcttcaga agtctttata ccatttataa cattagtaga aaagaatagt attgtaatgc
     1921 agacaaaaag tgataaaaca gcgtttattt tttttaacat attttgaatt tcctttcaaa
     1981 tgtaaataaa aaagagagtt taaactctct tttttaataa acattccacc aaccagtatt
     2041 tccagtaagt gtggcattgc atgttgcata agctcttact ccagctttct gaactgatcg
     2101 tacttgaccg cctcgtccat ttttagctgt tgcactatgt ttagctctag catggtagta
     2161 atttgcataa actttaaatc cattaacccc acagttgaaa tctccgccgc cagcagcata
     2221 tctcaattta ctagaagaaa atccttcttc tgtagcttcc attcctcctc cgtaaatggc
     2281 ggtatggtct tcgggtacat ctagatttaa agcttctgta gcactaacta ttcctccact
     2341 agttaataag acacccatta aagcagaaag acataaaaat tttttcataa cttatttctc
     2401 ctatcttttt tactagaatt attttatttt agtgtgtttt ataaaataaa gaactttttt
     2461 ggcgcgttag tatttttatg ataaaatttt atgtaaataa tcgtttacat aggagaaaaa
     2521 atggaggatt taggatctaa gctaagagaa ttaagagtga aaaaagggta tactcaagaa
     2581 tatgtggcaa gaaaatttaa tgttacacgt caaactattt caaagtggga aaataacaaa
     2641 acaacaccag atatagagac actaaaaaaa atttgtgaat tttatagtac taacataaat
     2701 tctattttaa atgaagataa tttaaaaaat aaacaaaata atttactggt attgacagct
     2761 attattttta atatatttgt tactatatta ggtttatttg aattgattca aatgaatttt
     2821 tttagcttta cacacggaat atttacttgg tttgctctat ctggattaat agtaatagat
     2881 atcttaaatt atttttatat ttactattct tatttaaaaa atgaaaaatt tttttataca
     2941 atattttttc ccctagtgat tttgattatt ttattaagct aatatgaagt agttagttat
     3001 ttaattgcat agttgacata atgtacagta tgctaagtag cgcctgtatc aatcgataca
     3061 ggggctcact ttgtgatttt taattggtct aggtcaaaag ctctttttaa ggcttgattt
     3121 tctgcagttg cttctttccg tttctttgtg aatttttacc agtcattttt tactggattg
     3181 ttatttttta taaaatatga taaaattttg gtagcgcttt ataagcgcgt tgctttttca
     3241 agaataaact ctcattattt ttttaactgg ctgctcgaaa gagcagtctt ttttattatt
     3301 tatccgtttc ttttacaaac aattcttcgt taggactgtt gtaataattt ggattcaatt
     3361 tcaattcacc atgtttattc cctttattga tcactgacaa taacatcgga ctgaagccta
     3421 gaattcctag taaaatagaa ccgaatagaa gattttttct tgtcatgaat attccctcca
     3481 gttatttcca aagttgccac catttttgct tcttctttgt gtgtttttct atcgattctg
     3541 tttcttcaga aagaatagta gttgattcat cttctggagt ttcttccgta taaactaatt
     3601 gtaactgttc ttgcagtttt tcgatctgtt gatttgcctg tagggtgagc tgttgttgct
     3661 ggtcgagtaa tttttgcata ttagaattat tttgctcttt aagggaaagt tgttctcgta
     3721 aaaattcatt ttcttcacgc aacttatcta gcaactcgtt tacaaccacg tttttgcttg
     3781 atatataaat gtttttatcg ttgtcttttt catactcatg caacctatct agtaaccttt
     3841 ggttacctaa gttgctagca agttgcaata ctccagaacg caatacaacc ttataaccac
     3901 gctcattttt agctatcatg gaagaatcta atttttggat atgtttcgat attgcttgcc
     3961 tactcatctt aaaaagatct gacaattcct ttattgtaaa ggtttcttgt ggttgcatac
     4021 tggttgcctc ctaggttgct actggaataa attatctaaa tcagataata aacttttttt
     4081 ctctttttcc gaaagtgcgg actctgtttg tttttcaaga gcttttttct ctttttctag
     4141 ttgtttgatt tgttggagga gttcaagttc tcgtttcttc tgtttgattt tttcgttaat
     4201 tgttgtagcg ggttcttcgt attctttttg tttatcagct aaagccaatg ctttattttg
     4261 gtcttctttg gttggtgcat gtttgttaaa gtcgtctgct tttttgatct caggtttgaa
     4321 cgtaaattga tagccaataa caggttttcc ccgtcctttt ccgtatttct ttttgattgt
     4381 tagtcctttg aagattgcag ttagttcttg acgtataggt tttataactc gtttatctat
     4441 atcaggttgc ttataacttt taggaatatc taataattca agaaagtctt ctttggagaa
     4501 atatgcgaaa cctttggttc tgtattgctt taacagacga aacatagtct ttgcatagcc
     4561 actttgtagc tcgttgaatt gttgtaaaga atagcgtacc cactcgtcta aattatttaa
     4621 caaaggcagt gctttttcgt aaattcgtat atctacgtaa ggattttcta cgtccccgtt
     4681 gattttaaat tctgtaaaca ttacaaacat atcgtaagaa agcccagact cacttctacg
     4741 accaaagcgt aaagataaga ttttttcata agttgctttt aaatcttcta caaatcgtac
     4801 atttgcagtt gctttgtagt tacttaaatc ttttagttgg tcaaacgtaa aacgaactgt
     4861 cttatcccct ttttcacgca ttctagacac gatggaaaaa aacaaattca tttcaaccgg
     4921 tgtaaatttt cttaaaggaa ttgtatttaa ttcatggtgg tattttacaa tctcattagc
     4981 catataatca cctcaatcca attatgaatg acatttttta aatgtcaaga cattcatatt
     5041 tgacgttcaa acagacaaaa tgtccttcat aaacagacaa aatgtccttc ataaacagac
     5101 aaaatgtcct tcataaacag acaaaatgtc cttcataaca ttctgtatcc attggtagag
     5161 taaggacaca gcttacggta aagaggttta aaaagaggtt taaaaagaga tataaaaaga
     5221 ggggggctaa ttttcccgcc taaaaaccac ttcttttttc tttaaacagg ttcgcgcatt
     5281 tttcaaaact gccaatccaa aaccttgctt cctacggtcg ccaaaaaggt gtgatcgcta
     5341 cgctctcaca ttgtactgcg cagtcgccta aaggctcctt tgctaaaaac atgttggtaa
     5401 agaggccttt gagcggttgt gaatgcttat ctaagccgtt ttaagacagt cttggataaa
     5461 aatccttatc tagctttaaa gcgccttaca ggcggtcagg gcaagcccta acaaccctaa
     5521 aataagcgat acttaaaaac accacacaag tttaattttt taagatataa taacttgtcg
     5581 cctatccgac agtcttgcga ctggaaggag gtgtatctaa tgtttgaatt gttcatttgc
     5641 ccccttctta tagaagtttg tgtctcttta ttcagttatt ggttgaataa ccgaaacaag
     5701 aagtaaaatc aataaaggcg acatcaacct gaaaaaaagc cccagagaat ccgacctctc
     5761 tggggctttc gtgtatctaa tgtttgatca tttgcatgtt tagtttaaca tgattgccga
     5821 tcaaattcaa ttttgctgtt gctgatctaa ccattccttg acactttcta gataatttaa
     5881 tgccgtttct tttttcgttg gactttgtat gttagttgct tgttgaaaca ggttttcgta
     5941 gcgttcatat gtgacagatt gttgtttttc ttttttttct tcttcactgg caaaccaagg
     6001 gtttgtgtct ccaaatagat cactacaggc ttcttctatc tttgtatctt ttacatattg
     6061 cacgtccttt tttacttgtt catatttttc ttggtattct ttttgcggtt cttctggcaa
     6121 attccgataa agtcgagtga gtgtttccat atttttatcc gtcaaacgct tttcttgctt
     6181 cacaaaaata tcattcatgt cctcataaca agcggtaaat ttttctattt tttgtgctaa
     6241 ggcttgctct tttttctttt tattacgtgc tgattcatac ttttgagtga catttcctaa
     6301 taaaatactt ctagaaacgc gcttattgcc ctctaaaacg cctaataagc cgcttgaaac
     6361 gattatcaag gaaaatatac agccaagcaa gaaaaatcgc ttaaatcgaa ttttagagcc
     6421 ttttaatgaa tatcttgttt ggaaaatttt atagatgagc caaatacttc ctgaaatact
     6481 aacaaaaatg agtagatagg gtaaataaaa tgccatttta gcctccccat actaaccaaa
     6541 atgcgaacag tgtcaggact aagcaacata agggggcaag aaaatccatt gatcgttgaa
     6601 tgaacgcctt tttttgttct ttatttcgct gatctaatag ctcttgatgg agttttttaa
     6661 cttgtaaatc aatcgtagtt tgtatccttt cttgtgtgta gggagcaaga aaagcacgtt
     6721 gtttctttat ttcgttttct atgtctttta acgttttaga gcgataattc ttgttggtgg
     6781 tctgttcctc tttcaacgtg ttgttcatgg tttcttcgag ccggtctaat ttccggttgt
     6841 tcaggcgtaa gagttcgttg ttctcttcgt acttcttctg cagtttcgcg aatgctagct
     6901 cgagctcttc gtagctcgtt ggttcgctgt tggttatagg ttcgttctcg tctgtccagc
     6961 tcatgtaaaa tcgtctcctt ttcgtagtcc tttcctagtc tgttggatcg tacttttttc
     7021 tttttgtgga tcaattcgta agtgaaattt ttcccgcgat tgaagcaatg aatgccgaat
     7081 gttttgaggg aatcacaaaa ttggtcatag gttctggttt tcttgttttt catggtttga
     7141 tcaatcgcaa accgaatttc atctttccaa acaggttcat ttttttcttt tattttgatt
     7201 tcagccatgg ttcgtttttc ttcttttttt cctcgttcag tgatacttaa accatgagtt
     7261 aaacaaattt cgtcgttgat atcgtaacac ttatctagga atgcttgacc atgtgcttga
     7321 aacttttttc ctgtttctaa attgaccgaa ttaatgacaa tgtgattgtg gatatgctct
     7381 ttatctgtgt gcgtgtaaat cgcgatttgg tattctggcg cgattttttc agcgtattct
     7441 ttccctagtt ggttggcttt ttcagcggta atttctcctg gtcgaaagga ctgaatcaat
     7501 aaatgtgctt gtacacgatc gtcttttcca taaagcatgc gtatttgttt catttcactt
     7561 ttggcataat caatgtcaca gtttattcct gttttgacgg ttgctcgtgg ttctgcgtaa
     7621 ttgattgctc ggctacatga agtggttcga cttactttaa ctgttgccat atcttttgta
     7681 gctccttttg catgattttt aggttttcgg ataaacgttg agcgtcacca ggagcaacgt
     7741 catgttggtg tgtattgcac cacttagcaa tttgattgat attccctcca gctttggcta
     7801 aatcgcgcgc gattgcctgg gcaatgtctg gcgccacttt cggtgtcacc agacgcgcgt
     7861 tttgtgcctt ggctttcacg aaagccggca cactcatttg caaagcttca gctgactgct
     7921 tcagctttgc aaattcggat tcgctcactc gaaaactgat ttgttttggc tcggatcgat
     7981 aggtatattt tttagaagga tcgctagcca aattttgatt ttgttcgctc ataagtccca
     8041 ccctttcagt acgtcctacg gacgtatcgt tcgcaatacc attataccta aaaaggtaca
     8101 atgagtaaag ctcactaaaa gggtttggca agctctatat ttgctagcca aatacctccg
     8161 tatttggtgc aaataaccgc ttgtcaaagg ggcgcaagct cccccttgaa tccccctcaa
     8221 aacccacgct ccacaataca aaaaaagcta gcaaatgcta gccacttttt gtaaagtttc
     8281 gcttatttca ccgaagatga aattgttgct ttgcaacaaa aaacataaat gcttttttgt
     8341 gtaaactact aactatttag ctaaaactga tagttttatt aaacattcag caatattttt
     8401 agccaatttt ttatcagact ttcggcgagt cattatgata atatgtccac tttttgttac
     8461 ttctaccaaa aatctagcaa taactctccc attcttcagt tgatttagtt tcataagtct
     8521 tttgtaattg acggtttaat tttttgggca attgcaaaga ataaataagt ctatcataaa
     8581 atttttcata ctcttttttc aataaaatca cttgggtgcc tcctttttat taagtgatta
     8641 tttaaatttt gggataggag agatggaata taaaataaat acccgtggtg ctagttttaa
     8701 atcagctagc atcacgggta aaaattataa ttctataata ttagttccat agtttagaac
     8761 atttaagtca tgtgttacta gtacaacggc ttttttttct ttttctgtta aatttttcag
     8821 aaagttcatt ataaaagttg agttttcttt atctaaattt cctgtaggtt catctgcaaa
     8881 taatatatca aatggtttta acatatttct aattaatgct attctttgtt gttctccacc
     8941 gcttaagaga aagatttttt cttttaagat atcttttggc aaagaaaaat ctgttaataa
     9001 attataaata tgatcattgg aaatattact gttaaacttt tttgctaatt cgaaattgta
     9061 ttgtactgtt tgattttcaa gaagagcata gttttgaaat aaatagctaa tatgatttct
     9121 tcgtagacgt tgtatatcat gtttagaaac tttcttttca aaatagaata agttaccggt
     9181 atattgttta tctattaggc ttaaattatt taacagagta gtttttccac taccactttt
     9241 tcctgttact ataataaaat ctttttctga tattgaaaag ttccaatttt gtttgatctt
     9301 ttttttatta attgacaggg tgaaattctc aactgataat atcataaaat attctcctta
     9361 aaatatttaa aactattttt tttataaata ataatagaag taattagatt tattaaaaag
     9421 atcataaaaa ttataaaata caagtaagtg ttttttgaaa atatcaattg aaaacacata
     9481 gtagcaaata actctattgt aaaggga
//
