LOCUS       NZ_AP024835             6302 bp    DNA     circular CON 04-NOV-2023
DEFINITION  Enterococcus faecium strain AA620 plasmid pAA620-4, complete
            sequence.
ACCESSION   NZ_AP024835
VERSION     NZ_AP024835.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00324535
            Sequence Read Archive: DRR305791
            Assembly: GCF_019977495.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Hashimoto,Y. and Tomita,H.
  TITLE     Molecular characterization of the VanD-type vancomycin resistant
            Enterococcus faecium clinical isolates from a patient after
            vancomycin therapy
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6302)
  AUTHORS   Hashimoto,Y. and Tomita,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUL-2021) Contact:Yusuke Hashimoto Gunma University
            Graduate School of Medicine, Bacteriology; 3-39-22, Maebashi, Gunma
            371-0034, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: canu v. 2.1.1
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Illumina Miseq; Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/04/2023 02:38:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,078
            Genes (coding)                    :: 2,876
            CDSs (with protein)               :: 2,876
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 202
            CDSs (without protein)            :: 202
            Pseudo Genes (ambiguous residues) :: 0 of 202
            Pseudo Genes (frameshifted)       :: 135 of 202
            Pseudo Genes (incomplete)         :: 63 of 202
            Pseudo Genes (internal stop)      :: 48 of 202
            Pseudo Genes (multiple problems)  :: 37 of 202
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP024835.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="AA620"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pAA620-4"
                     /geo_loc_name="Japan"
                     /collection_date="2014"
     gene            complement(join(6238..6302,1..214))
                     /locus_tag="LDN34_RS15690"
     CDS             complement(join(6238..6302,1..214))
                     /locus_tag="LDN34_RS15690"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_224168523.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLVG
                     VIFALSIVLSGLFGVLEGNKRVSRSILLGISLKSMKQHVIKRKKSKH"
                     /name="hypothetical protein"
     gene            complement(221..499)
                     /locus_tag="LDN34_RS15695"
                     /old_locus_tag="GVanDAA620_31860"
     CDS             complement(221..499)
                     /locus_tag="LDN34_RS15695"
                     /old_locus_tag="GVanDAA620_31860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_058138385.1"
                     /translation="MNEEQTTNKNYRSKTLKDIENEIKRQRAFLAPYTQERIQTTMDSQ
                     VKKLHQALLDQRNQEQKKALIQRSMDILAPLCCLVLTLLAFWLVWGE"
                     /name="hypothetical protein"
     gene            complement(459..1373)
                     /locus_tag="LDN34_RS15700"
                     /old_locus_tag="GVanDAA620_31870"
     CDS             complement(459..1373)
                     /locus_tag="LDN34_RS15700"
                     /old_locus_tag="GVanDAA620_31870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002321380.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRT
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKLKEK
                     NEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKKKVRS
                     NKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTPEQSEI
                     RPTRRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1355..1735)
                     /locus_tag="LDN34_RS15705"
                     /old_locus_tag="GVanDAA620_31880"
     CDS             complement(1355..1735)
                     /locus_tag="LDN34_RS15705"
                     /old_locus_tag="GVanDAA620_31880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_002321381.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPDDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(2057..2260)
                     /locus_tag="LDN34_RS15710"
                     /old_locus_tag="GVanDAA620_31890"
     CDS             complement(2057..2260)
                     /locus_tag="LDN34_RS15710"
                     /old_locus_tag="GVanDAA620_31890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002321382.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQL
                     YKSGLFLVLILVVLDIIKSKKK"
                     /name="hypothetical protein"
     gene            complement(2272..2805)
                     /locus_tag="LDN34_RS15715"
                     /old_locus_tag="GVanDAA620_31900"
     CDS             complement(2272..2805)
                     /locus_tag="LDN34_RS15715"
                     /old_locus_tag="GVanDAA620_31900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002334266.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENVV
                     IEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAINAA
                     FWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQRGY
                     DAAGRVVATRYSTL"
                     /name="hypothetical protein"
     gene            complement(3255..3425)
                     /locus_tag="LDN34_RS15720"
                     /old_locus_tag="GVanDAA620_31910"
     CDS             complement(3255..3425)
                     /locus_tag="LDN34_RS15720"
                     /old_locus_tag="GVanDAA620_31910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002292472.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(3441..3974)
                     /locus_tag="LDN34_RS15725"
                     /old_locus_tag="GVanDAA620_31920"
     CDS             complement(3441..3974)
                     /locus_tag="LDN34_RS15725"
                     /old_locus_tag="GVanDAA620_31920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002321384.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLREQL
                     ALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATETID
                     KQIDTEKKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            complement(3995..4945)
                     /locus_tag="LDN34_RS15730"
                     /old_locus_tag="GVanDAA620_31930"
     CDS             complement(3995..4945)
                     /locus_tag="LDN34_RS15730"
                     /old_locus_tag="GVanDAA620_31930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_033642128.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRL
                     TFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIRGT
                     AKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFAYFS
                     KEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVVGYQF
                     TFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQI
                     KQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
                     /name="replication initiation protein"
     gene            5621..5707
                     /locus_tag="LDN34_RS15735"
     CDS             5621..5707
                     /locus_tag="LDN34_RS15735"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_107532029.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(5828..6229)
                     /locus_tag="LDN34_RS15740"
     CDS             complement(5828..6229)
                     /locus_tag="LDN34_RS15740"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_224168522.1"
                     /translation="MEEFTACYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEV
                     QEKYEQVKKDVQYFKDTQTEEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQA
                     TNIQNPTKKETALNYLESVKEWLDQQQQN"
                     /name="hypothetical protein"
     Typing          complement(3996..4944)
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.953"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(463..1373)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.079"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaatgcttct tgaaacacgt ttattaccct ctaaaacgcc gaataaacca cttaaaacga
       61 ttgataaggc aaatataacc ccgaccaata aaaatcgctt aaatcgaatt ttagagcctt
      121 ttaatgaata tcttgtttga taagccttat agctaagcca aataattcct aaaatactgg
      181 cagaaatgag tagatagggt aaatagaatg ccatgtctgt ttattctccc cataccaacc
      241 aaaaggctaa taatgttagt actaagcaac ataagggggc aagaatatcc atcgatcgtt
      301 gaattaaagc ctttttttgt tcttggtttc gctggtctaa tagcgcttgg tggagttttt
      361 tgacttgtga atccatcgta gtttgtatcc tttcttgggt ataaggcgca agaaaggcac
      421 gttgtctctt tatttcgttt tcgatgtctt ttaacgtttt agagcgataa ttcttgttgg
      481 tggtctgttc ctcgttcaac gtgttgttca tggtttcttc gagtcggtct aatttccgat
      541 tgttcaggcg taaaagttcg ttgttctctt cgtacttctt ctgcagtttc gcgaatgcta
      601 gctcgagctc ttcgtagctc gttggttcgt tgttgattgt aggttcgttc tcgtctgtcc
      661 agctcatgta aaatcgtctc cttttcgtaa tcctttccta gtttgttgga tcgtactttt
      721 ttctttttgt ggaccaattc gtaagtaaaa ttttttccgc gattcaagca atgaatgccg
      781 aatgttttaa gtgaatcaca aaattgggta tacgttcgag tttttttgtt tttcattgtt
      841 tgatcaatcg caaaccgaat ttcgtctttc caaaccggtt catttttttc ttttaattta
      901 atttcagaca tggttcgttt ttcggttttc tttcctcgtt ccgtgattgt taagtcatgt
      961 tcaagacaaa tctcgtcatt taggtcataa catttgttta aaaatgcttg cccatgggct
     1021 tgaaattttt tcccagtttc aagattaacg gagttaatga caatgtgatt atgaatatgg
     1081 tctttgtctg tatgtgtgta aatcgcgatt tggtggttag gggctatttt tttcgcatac
     1141 tctgccccta gttgattggc tttttcagcg gtaatttctc ctggtcgaaa ggactgaatc
     1201 aataagtgtg cttgtacacg atcgtctttt ccataaagcg tgcgtatttg tttcatttca
     1261 cttttggcat aatcaatgtc acagtttatt cctgttttga cggttgctcg tggttctgcg
     1321 taattgattg ctcggctaca tgaagtggtt cgacttactt taactgttgc catatctttt
     1381 gtagctcctt ttgcatgatt tttaggtttt cggataaacg ttgagcatca tcaggagcaa
     1441 cgtcgtgttg gtgtgtattg caccacttgg caatttgatt gatattccct ccagctttgg
     1501 ctaaatcgcg cgcgattgcc tgggcaatgt ctggcgccac tttcggtgtc accagacgcg
     1561 cgttttgtgc cttggctttc acgaaagccg gcacactcat ttgcaaagct tcagctgact
     1621 gcttcagctt tgcaaattcg gattcgctca ctcgaaaact gatttgtttt ggctcggatc
     1681 gataggtata ttttttagaa tgatcgctag ccaaattttg atgttgttcg ctcataagtc
     1741 ccaccctttc agtacgtcct acggacgtat cgttcgcaat accattgtac ctaaaaaggt
     1801 acaatgagta aagctcacta aaagggtttg gcaagctcta tatttgctag ccaaatacct
     1861 ccgtatttgg tgcaaataac cgcttgtcaa aggagcgcaa gctccccctt gaatccccct
     1921 caaaatccac gctccacaat acaaaaaaag gctagcattt gctagccact ttttgtaaag
     1981 tttcgcttat ttcaccgaag gtgaaattgt tgctttgtaa tattcaaaca accctattct
     2041 tctaaaatgt atcgatttat ttttttttag actttattat gtctaagaca actaagatta
     2101 acactaaaaa taaaccagat ttgtataatt gccctccgct tagcatatta acaattatag
     2161 caacagctat agcaaatata aaaatacgaa tagtagctat ttgcggattc tttttagtaa
     2221 atgtttttaa tgattttaat gatttagata tcaaaaacat cactccttaa cttataacgt
     2281 tgaatatcta gtagctacaa ctcttccagc tgcatcatat cccctttggt aataaaatcc
     2341 aatccagcca ttaccatttt tgtcccattc ttttgcaact gcattccctg gagaagcacc
     2401 ttttttagtc caacttgcac ctgttaacaa agcaccaatt gcccaacctg ggataccaaa
     2461 catagaagtg ctagctttcc ctcctgccca aaaggcagca ttaatagcat tagcagctac
     2521 tccagtggtt acagcaatat ttgtatattc ctttttgccc catcttgcaa ctgcagctct
     2581 tgtcatgata tctgaagaat ttgcaaaatc ttccgcacta taaccatcct catatttagt
     2641 tacgaccata ccatcttcag tgtactcaat aactacattt tcagtagcat tgttttcgtt
     2701 taatgatacc acttgggaat tttctgaagc ataaactata gtagttgacg gtaaaacaac
     2761 tgccccaata gttaacaaag ctacccctaa agtaaatttt ttcatagaat ttcctcctga
     2821 tacagaaaaa tatatagaaa acgttttctt tgtgaaaatc ataaaacaaa aggtataggc
     2881 ttgtcaatat tttatttagt aaagataatt tatatttttg ataatgtggt atagttagaa
     2941 tgcattagga aaatatgtta tattaaattc ttgttgacat aatgtacact atgccaagta
     3001 gcccttgtat caaccgatac agaggctcac tttgtgattt tcgattggtc taggtcaaaa
     3061 gctcttttta agcttgattt tctgcggttg cttctttccg tttctttgtg aatttttacc
     3121 agtcattttt tactgggttg ttatttttaa taaaatatga taaatttttg gtagcgcttc
     3181 ataagcgcgt tgctttttca agaataaaca ctcattattt tttaactggc tgctcgaaag
     3241 agcagtcttt tttattattt atctgtttct tttacaaaca attcttcgtt aggactgttg
     3301 taataatttg gattcaattt caattcacca tgtttattcc ctttattgat cactgacaat
     3361 aacatcggac tgaatcctag aattcctagt aaaatagaac cgaatagaag attttttctt
     3421 gtcatgaatg ttccctccag ttatttccaa agttgccacc attttttttc agtatcaatt
     3481 tgtttgtcta ttgtttctgt tgcttcagaa agagtcgttg agtcattctc tggagcttct
     3541 tctgtataag tcaactgtag tttttcttgc agtttttcga tttgtttgtt ggcttgtagg
     3601 gtgagttgtt gttgctggtc gagtagtttt tgtatatgag aattattttg ctctctgagg
     3661 gcaagttgtt ctcgtaaaaa ttcattttct tcacgcaact gatctagcaa cttgtttgta
     3721 accacgtttt tgcttggtat attaacattt ttatcgttgt cttgttcgta ctcgtgcaac
     3781 ctatctagca acatttggtt gcctaagttg cgagcaagtt gcaatactcc agaacgcaat
     3841 acaaccttat aaccacgctc atttttagct atcatggaag aatctaattt ttggatatgt
     3901 ttcgatattg cttgcctact catcttaaaa agttctgaca attcctttat tgtaaaggtt
     3961 tcttgtggtt gcatactggt tgcctcctag gttgctactg gaataaatta tctaaatcag
     4021 ataataaact ttttttctct tttttcgaaa gtgcggactc tgtttgtttt tcaagagctt
     4081 ctttctcttt ttctagttgt ttgatttgtt ggaggagttc aagttctcgt ttcttctgtt
     4141 tgattttttc gttaattgtt gtagcaggtt cttcgtattc tttttgttta tcagctaaag
     4201 ccaatgcttt attttgttct tctttggttg gtgcatgttt attaaaatca tctgcttttt
     4261 tgatttctgg tttaaacgta aattgatagc caacgacagg ttttcctcgc ccttttccgt
     4321 atttcttttt gattgataat cctttgaaga ttgcggttaa ttcctgtcgt ataggcttta
     4381 taacacgatt atcaacgttt gcttggttgt tccagtaact ttttggaata tctaatagtt
     4441 catgaaaatc ctctttagaa aaataagcaa aacctttagt tcgatattgt tttagtaatc
     4501 gaaacatagt ctttgcatag ctactttgta attcgttaaa ttgttggagt gaatagcgaa
     4561 cccactcatc taaattgttt agtaaaggta aagctttttc gtaaacttgt atgtctacat
     4621 atggatcttt agcagttcca cgtattttaa actcactaaa cataacgaat ctatctatat
     4681 cgagcccatt agcacttctt ttaccaaaac gtaaatccat taatttatca taagtcattt
     4741 ctaaatcatt aatgaaacga ttattagcag ttgctttata attacttaaa tcttttagtt
     4801 ggtcaaaagt aagacggact gttttatcgc ctttttcgcg cattctagac acaatagaga
     4861 aaaatagatt catttcaact ggtgtaaatt tccgcaacgg aatggtattt aattcatgat
     4921 ggtatttcac gatttcattt gccaaagata ttcctcctta ctccttcaat ggatacagac
     4981 gttataaaca acgttttgtc tgtgataatg catattataa catacgtgag tgcaaaaaaa
     5041 gagtttaaaa gcagacaaaa tgtagtttaa aagcagacaa aatgtagttt aaaagcagac
     5101 aaaatgtagt ttaaaagcag acaaaatgta gtttataaaa cgctgtatcc attgaaggag
     5161 taaggacaca gccgacggta aagaggttta aaaaaaggtt taaaaagaga ttataaaaga
     5221 gggtgacaaa atttcccgcc taaaaaccac ttcttttttc taccaacagg ttcgcgcatt
     5281 tttcaaaact gccaattcaa aaccttgctc cctacggtcg ctaaaaaagt gtgatcgcta
     5341 tgctctcaca ttgtactgcg cagtcgccta aaggctcctt tgctaaaaac aagtcagtaa
     5401 cggaggtggg ggaagcctct gatagctcgt ctaagtcgtt ttaagacagt cttgggtaaa
     5461 aaaccttatc ttgttcttaa agcgccttac aggcggtcag ggcaagccct aacaacccga
     5521 aaataaggga tacttaaaaa caccacacag attttatttt gtaaggtata ataacttgtc
     5581 gcctatccga cagtcttgcg actggaagga ggtgtatcta atgtttgaac ggttcatttg
     5641 cccccttctt gtagaagttt gtgtctcttt attcagttat tggttgaata accgaaacaa
     5701 gaagtaaaat caataaaggc gacatcaacc tgaaaaaaag ccccagagaa tccgacctct
     5761 ctggggcttt cgtgtatcta atgtttgatc atttgcaggt tcagtttaac atgattgcta
     5821 atcaaattca attttgttgt tgctgatcca accattcctt gacactttct agataattta
     5881 atgccgtttc ttttttcgtt ggattttgta tgtttgttgc ttgctgaagc agtgtttcat
     5941 aacgttcgta tgtgaccgat tgttgccgtt cttttcgttc ttgttcactg gttgaaaaag
     6001 ggattgtatc gctaaataag ttacaacaag cttcttctgt ctgcgtatct ttaaaatatt
     6061 gcacgtcttt ttttacttgc tcatattttt cttgtacctc tttttgctgt ttttctggta
     6121 agttttgata aaaacgagtg agttttttca tgtttttatc tgttaaacgt ttctcttgat
     6181 ccacaaaaat attgttcatg tcctcataac aagcggtaaa ttcctccatt ttttgtgcta
     6241 atgcttgctc ttttttcttt ttatcacatg ctgcttcata cttttgagtg atatccctaa
     6301 ta
//
