LOCUS       NZ_AP024834             9507 bp    DNA     circular CON 04-NOV-2023
DEFINITION  Enterococcus faecium strain AA620 plasmid pAA620-3, complete
            sequence.
ACCESSION   NZ_AP024834
VERSION     NZ_AP024834.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00324535
            Sequence Read Archive: DRR305791
            Assembly: GCF_019977495.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Hashimoto,Y. and Tomita,H.
  TITLE     Molecular characterization of the VanD-type vancomycin resistant
            Enterococcus faecium clinical isolates from a patient after
            vancomycin therapy
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9507)
  AUTHORS   Hashimoto,Y. and Tomita,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUL-2021) Contact:Yusuke Hashimoto Gunma University
            Graduate School of Medicine, Bacteriology; 3-39-22, Maebashi, Gunma
            371-0034, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: canu v. 2.1.1
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Illumina Miseq; Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/04/2023 02:38:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,169
            CDSs (total)                      :: 3,078
            Genes (coding)                    :: 2,876
            CDSs (with protein)               :: 2,876
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 202
            CDSs (without protein)            :: 202
            Pseudo Genes (ambiguous residues) :: 0 of 202
            Pseudo Genes (frameshifted)       :: 135 of 202
            Pseudo Genes (incomplete)         :: 63 of 202
            Pseudo Genes (internal stop)      :: 48 of 202
            Pseudo Genes (multiple problems)  :: 37 of 202
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP024834.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="AA620"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pAA620-3"
                     /geo_loc_name="Japan"
                     /collection_date="2014"
     gene            complement(join(9481..9507,1..510))
                     /locus_tag="LDN34_RS15630"
     CDS             complement(join(9481..9507,1..510))
                     /locus_tag="LDN34_RS15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002303560.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLASNLGNQRLLDRLHEYEKDNDKNIYISSKNVVVNELLDKLREENEFLREQL
                     SLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLVYTEETPEDESTTILSEETESI
                     EKHTKKKQKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            complement(531..1475)
                     /locus_tag="LDN34_RS15635"
                     /old_locus_tag="GVanDAA620_31750"
     CDS             complement(531..1475)
                     /locus_tag="LDN34_RS15635"
                     /old_locus_tag="GVanDAA620_31750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002303559.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRF
                     TFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKINGD
                     VENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFAYFS
                     KEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGYQFTF
                     KPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQIKQ
                     LEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
                     /name="replication initiation protein"
     gene            2112..2198
                     /locus_tag="LDN34_RS15640"
     CDS             2112..2198
                     /locus_tag="LDN34_RS15640"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_106914087.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(2319..3008)
                     /locus_tag="LDN34_RS15645"
                     /old_locus_tag="GVanDAA620_31760"
     CDS             complement(2319..3008)
                     /locus_tag="LDN34_RS15645"
                     /old_locus_tag="GVanDAA620_31760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002303565.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFLLG
                     CIFSLIIVSSGLLGVLEGNKRVSRSILLGNVTQKYESARNKKKKEQALAQKIEKFTACY
                     EDMNDIFVKQEKRLTDKNMETLTRLYRNLPEEPQKEYQEKYEQVKKDVQYVKDTKIEEA
                     CSDLFGDTNPWFASEEEKKEKQQSVTYERYENLFQQATNIQSPTKKETALNYLESVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(3010..3288)
                     /locus_tag="LDN34_RS15925"
     CDS             complement(3010..3288)
                     /locus_tag="LDN34_RS15925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_033627308.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLAPYTQERIQTTIDLQ
                     VKKLHQELLDQRNKEQKKAFIQRSMDFLAPLCCLVLTLFAFWLVWGG"
                     /name="hypothetical protein"
     gene            complement(3248..4162)
                     /locus_tag="LDN34_RS15650"
                     /old_locus_tag="GVanDAA620_31770"
     CDS             complement(3248..4162)
                     /locus_tag="LDN34_RS15650"
                     /old_locus_tag="GVanDAA620_31770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002303564.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGKEYAEKIAPEYQIAIYTHTDKEHIHNHI
                     VINSVNLETGKKFQAHGQAFLDKCYDINDEICLTHGLSITERGKKEEKRTMAEIKIKEK
                     NEPVWKDEIRFAIDQTMKNKKTRTYDQFCDSLKTFGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4144..4524)
                     /locus_tag="LDN34_RS15655"
                     /old_locus_tag="GVanDAA620_31780"
     CDS             complement(4144..4524)
                     /locus_tag="LDN34_RS15655"
                     /old_locus_tag="GVanDAA620_31780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_002292464.1"
                     /translation="MSEQNQNLASDPSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPGDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(4966..5103)
                     /locus_tag="LDN34_RS15660"
                     /old_locus_tag="GVanDAA620_31800"
     CDS             complement(4966..5103)
                     /locus_tag="LDN34_RS15660"
                     /old_locus_tag="GVanDAA620_31800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002303562.1"
                     /translation="MILLKKEYEKFYDRLIYSLQLPKKLNRQLQKTYETKSTEEWESYC
                     "
                     /name="hypothetical protein"
     gene            complement(5217..5837)
                     /locus_tag="LDN34_RS15665"
                     /old_locus_tag="GVanDAA620_31810"
     CDS             complement(5217..5837)
                     /locus_tag="LDN34_RS15665"
                     /old_locus_tag="GVanDAA620_31810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_002303561.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTLL
                     NNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAKKF
                     NSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEPTGN
                     LDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
                     /name="ABC transporter ATP-binding protein"
     gene            complement(5905..7959)
                     /locus_tag="LDN34_RS15670"
                     /old_locus_tag="GVanDAA620_31820"
     CDS             complement(5905..7959)
                     /locus_tag="LDN34_RS15670"
                     /old_locus_tag="GVanDAA620_31820"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_224168521.1"
                     /translation="MLKKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQY
                     KDDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILARN
                     DSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLKKYG
                     TPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNSFDTI
                     KDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIVTLSIY
                     HLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINIHNEAENNSLKYWERTT
                     NLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQENNKEFFI
                     GQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTTYILVPKKY
                     KEYAKMIEKNYLEDIQFRLEDSPPQAPYKTPNLKNLKIKLIFVNNNQKYFSFNTLYGKA
                     KDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQINPYIKEFNL
                     ENIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIFVSFTINSKKIA
                     LKKIFGYSPISQVLDLCLLPFTIELFATMCFQLIFSKNTYLYFIIFMIF"
                     /name="hypothetical protein"
     gene            complement(8013..8387)
                     /locus_tag="LDN34_RS15675"
                     /old_locus_tag="GVanDAA620_31830"
     CDS             complement(8013..8387)
                     /locus_tag="LDN34_RS15675"
                     /old_locus_tag="GVanDAA620_31830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_000713874.1"
                     /translation="MKKFLCLSALMGVLLTSGGIVSATEALNLDVPEDHTAIYGGGMEA
                     TEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKAGV
                     RAYATCNATLTGNTGWWNVY"
                     /name="lactococcin 972 family bacteriocin"
     gene            8520..8981
                     /locus_tag="LDN34_RS15680"
                     /old_locus_tag="GVanDAA620_31840"
     CDS             8520..8981
                     /locus_tag="LDN34_RS15680"
                     /old_locus_tag="GVanDAA620_31840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000389746.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPDI
                     ETLKKICEFYSTNINSILNEDNLKNKQNNLLVLTAIIFNIFVTILGLFELIQMNFFSFT
                     HGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTIFFPLVILIILLS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(9295..9465)
                     /locus_tag="LDN34_RS15685"
                     /old_locus_tag="GVanDAA620_31850"
     CDS             complement(9295..9465)
                     /locus_tag="LDN34_RS15685"
                     /old_locus_tag="GVanDAA620_31850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002292472.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     BGC             3013..9507
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(532..1475)
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3252..4162)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.684"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cttcttcttt gtgtgttttt ctatcgattc tgtttcttca gaaagaatag tagttgattc
       61 atcttctgga gtttcttccg tataaactaa ttgtaactgt tcttgcagtt tttcgatctg
      121 ttgatttgcc tgtagggtga gctgttgttg ctggtcgagt aatttttgca tattagaatt
      181 attttgctct ttaagggaaa gttgttctcg taaaaattca ttttcttcac gcaacttatc
      241 tagcaactcg tttacaacca cgtttttgct tgatatataa atgtttttat cgttgtcttt
      301 ttcatactca tgcaacctat ctagtaacct ttggttacct aagttgctag caagttgcaa
      361 tactccagaa cgcaatacaa ccttataacc acgctcattt ttagctatca tggaagaatc
      421 taatttttgg atatgtttcg atattgcttg cctactcatc ttaaaaagat ctgacaattc
      481 ctttattgta aaggtttctt gtggttgcat actggttgcc tcctaggttg ctactggaat
      541 aaattatcta aatcagataa taaacttttt ttctcttttt ccgaaagtgc ggactctgtt
      601 tgtttttcaa gagctttttt ctctttttct agttgtttga tttgttggag gagttcaagt
      661 tctcgtttct tctgtttgat tttttcgtta attgttgtag cgggttcttc gtattctttt
      721 tgtttatcag ctaaagccaa tgctttattt tggtcttctt tggttggtgc atgtttgtta
      781 aagtcgtctg cttttttgat ctcaggtttg aacgtaaatt gatagccaat aacaggtttt
      841 ccccgtcctt ttccgtattt ctttttgatt gttagtcctt tgaagattgc agttagttct
      901 tgacgtatag gttttataac tcgtttatct atatcaggtt gcttataact tttaggaata
      961 tctaataatt caagaaagtc ttctttggag aaatatgcga aacctttggt tctgtattgc
     1021 tttaacagac gaaacatagt ctttgcatag ccactttgta gctcgttgaa ttgttgtaaa
     1081 gaatagcgta cccactcgtc taaattattt aacaaaggca gtgctttttc gtaaattcgt
     1141 atatctacgt aaggattttc tacgtccccg ttgattttaa attctgtaaa cattacaaac
     1201 atatcgtaag aaagcccaga ctcacttcta cgaccaaagc gtaaagataa gattttttca
     1261 taagttgctt ttaaatcttc tacaaatcgt acatttgcag ttgctttgta gttacttaaa
     1321 tcttttagtt ggtcaaacgt aaaacgaact gtcttatccc ctttttcacg cattctagac
     1381 acgatggaaa aaaacaaatt catttcaacc ggtgtaaatt ttcttaaagg aattgtattt
     1441 aattcatggt ggtattttac aatctcatta gccatataat cacctcaatc caattatgaa
     1501 tgacattttt taaatgtcaa gacattcata tttgacgttc aaacagacaa aatgtccttc
     1561 ataaacagac aaaatgtcct tcataaacag acaaaatgtc cttcataaac agacaaaatg
     1621 tccttcataa cattctgtat ccattggtag agtaaggaca cagcttacgg taaagaggtt
     1681 taaaaagagg tttaaaaaga gatataaaaa gaggggggct aattttcccg cctaaaaacc
     1741 acttcttttt tctttaaaca ggttcgcgca tttttcaaaa ctgccaatcc aaaaccttgc
     1801 ttcctacggt cgccaaaaag gtgtgatcgc tacgctctca cattgtactg cgcagtcgcc
     1861 taaaggctcc tttgctaaaa acatgttggt aaagaggcct ttgagcggtt gtgaatgctt
     1921 atctaagccg ttttaagaca gtcttggata aaaatcctta tctagcttta aagcgcctta
     1981 caggcggtca gggcaagccc taacaaccct aaaataagcg atacttaaaa acaccacaca
     2041 agtttaattt tttaagatat aataacttgt cgcctatccg acagtcttgc gactggaagg
     2101 aggtgtatct aatgtttgaa ttgttcattt gcccccttct tatagaagtt tgtgtctctt
     2161 tattcagtta ttggttgaat aaccgaaaca agaagtaaaa tcaataaagg cgacatcaac
     2221 ctgaaaaaaa gccccagaga atccgacctc tctggggctt tcgtgtatct aatgtttgat
     2281 catttgcatg tttagtttaa catgattgcc gatcaaattc aattttgctg ttgctgatct
     2341 aaccattcct tgacactttc tagataattt aatgccgttt cttttttcgt tggactttgt
     2401 atgttagttg cttgttgaaa caggttttcg tagcgttcat atgtgacaga ttgttgtttt
     2461 tctttttttt cttcttcact ggcaaaccaa gggtttgtgt ctccaaatag atcactacag
     2521 gcttcttcta tctttgtatc ttttacatat tgcacgtcct tttttacttg ttcatatttt
     2581 tcttggtatt ctttttgcgg ttcttctggc aaattccgat aaagtcgagt gagtgtttcc
     2641 atatttttat ccgtcaaacg cttttcttgc ttcacaaaaa tatcattcat gtcctcataa
     2701 caagcggtaa atttttctat tttttgtgct aaggcttgct cttttttctt tttattacgt
     2761 gctgattcat acttttgagt gacatttcct aataaaatac ttctagaaac gcgcttattg
     2821 ccctctaaaa cgcctaataa gccgcttgaa acgattatca aggaaaatat acagccaagc
     2881 aagaaaaatc gcttaaatcg aattttagag ccttttaatg aatatcttgt ttggaaaatt
     2941 ttatagatga gccaaatact tcctgaaata ctaacaaaaa tgagtagata gggtaaataa
     3001 aatgccattt tagcctcccc atactaacca aaatgcgaac agtgtcagga ctaagcaaca
     3061 taagggggca agaaaatcca ttgatcgttg aatgaacgcc tttttttgtt ctttatttcg
     3121 ctgatctaat agctcttgat ggagtttttt aacttgtaaa tcaatcgtag tttgtatcct
     3181 ttcttgtgtg tagggagcaa gaaaagcacg ttgtttcttt atttcgtttt ctatgtcttt
     3241 taacgtttta gagcgataat tcttgttggt ggtctgttcc tctttcaacg tgttgttcat
     3301 ggtttcttcg agccggtcta atttccggtt gttcaggcgt aagagttcgt tgttctcttc
     3361 gtacttcttc tgcagtttcg cgaatgctag ctcgagctct tcgtagctcg ttggttcgct
     3421 gttggttata ggttcgttct cgtctgtcca gctcatgtaa aatcgtctcc ttttcgtagt
     3481 cctttcctag tctgttggat cgtacttttt tctttttgtg gatcaattcg taagtgaaat
     3541 ttttcccgcg attgaagcaa tgaatgccga atgttttgag ggaatcacaa aattggtcat
     3601 aggttctggt tttcttgttt ttcatggttt gatcaatcgc aaaccgaatt tcatctttcc
     3661 aaacaggttc atttttttct tttattttga tttcagccat ggttcgtttt tcttcttttt
     3721 ttcctcgttc agtgatactt aaaccatgag ttaaacaaat ttcgtcgttg atatcgtaac
     3781 acttatctag gaatgcttga ccatgtgctt gaaacttttt tcctgtttct aaattgaccg
     3841 aattaatgac aatgtgattg tggatatgct ctttatctgt gtgcgtgtaa atcgcgattt
     3901 ggtattctgg cgcgattttt tcagcgtatt ctttccctag ttggttggct ttttcagcgg
     3961 taatttctcc tggtcgaaag gactgaatca ataaatgtgc ttgtacacga tcgtcttttc
     4021 cataaagcat gcgtatttgt ttcatttcac ttttggcata atcaatgtca cagtttattc
     4081 ctgttttgac ggttgctcgt ggttctgcgt aattgattgc tcggctacat gaagtggttc
     4141 gacttacttt aactgttgcc atatcttttg tagctccttt tgcatgattt ttaggttttc
     4201 ggataaacgt tgagcgtcac caggagcaac gtcatgttgg tgtgtattgc accacttagc
     4261 aatttgattg atattccctc cagctttggc taaatcgcgc gcgattgcct gggcaatgtc
     4321 tggcgccact ttcggtgtca ccagacgcgc gttttgtgcc ttggctttca cgaaagccgg
     4381 cacactcatt tgcaaagctt cagctgactg cttcagcttt gcaaattcgg attcgctcac
     4441 tcgaaaactg atttgttttg gctcggatcg ataggtatat tttttagaag gatcgctagc
     4501 caaattttga ttttgttcgc tcataagtcc caccctttca gtacgtccta cggacgtatc
     4561 gttcgcaata ccattatacc taaaaaggta caatgagtaa agctcactaa aagggtttgg
     4621 caagctctat atttgctagc caaatacctc cgtatttggt gcaaataacc gcttgtcaaa
     4681 ggggcgcaag ctcccccttg aatccccctc aaaacccacg ctccacaata caaaaaaagc
     4741 tagcaaatgc tagccacttt ttgtaaagtt tcgcttattt caccgaagat gaaattgttg
     4801 ctttgcaaca aaaaacataa atgctttttt gtgtaaacta ctaactattt agctaaaact
     4861 gatagtttta ttaaacattc agcaatattt ttagccaatt ttttatcaga ctttcggcga
     4921 gtcattatga taatatgtcc actttttgtt acttctacca aaaatctagc aataactctc
     4981 ccattcttca gttgatttag tttcataagt cttttgtaat tgacggttta attttttggg
     5041 caattgcaaa gaataaataa gtctatcata aaatttttca tactcttttt tcaataaaat
     5101 cacttgggtg cctccttttt attaagtgat tatttaaatt ttgggatagg agagatggaa
     5161 tataaaataa atacccgtgg tgctagtttt aaatcagcta gcatcacggg taaaaattat
     5221 aattctataa tattagttcc atagtttaga acatttaagt catgtgttac tagtacaacg
     5281 gctttttttt ctttttctgt taaatttttc agaaagttca ttataaaagt tgagttttct
     5341 ttatctaaat ttcctgtagg ttcatctgca aataatatat caaatggttt taacatattt
     5401 ctaattaatg ctattctttg ttgttctcca ccgcttaaga gaaagatttt ttcttttaag
     5461 atatcttttg gcaaagaaaa atctgttaat aaattataaa tatgatcatt ggaaatatta
     5521 ctgttaaact tttttgctaa ttcgaaattg tattgtactg tttgattttc aagaagagca
     5581 tagttttgaa ataaatagct aatatgattt cttcgtagac gttgtatatc atgtttagaa
     5641 actttctttt caaaatagaa taagttaccg gtatattgtt tatctattag gcttaaatta
     5701 tttaacagag tagtttttcc actaccactt tttcctgtta ctataataaa atctttttct
     5761 gatattgaaa agttccaatt ttgtttgatc ttttttttat taattgacag ggtgaaattc
     5821 tcaactgata atatcataaa atattctcct taaaatattt aaaactattt tttttataaa
     5881 taataataga agtaattaga tttattaaaa gatcataaaa attataaaat acaagtaagt
     5941 gttttttgaa aatatcaatt gaaaacacat agtagcaaat aactctattg taaaagggag
     6001 taaacataaa tctaaaactt gacttattgg tgaatatcca aatatttttt ttaaagctat
     6061 tttttttgaa tttatagtaa aactaacaaa aatcatttga aaattaatta ttataaataa
     6121 tcctaatatt aaaagatatt gtagatatgc atttaattta tttacttttt tttgagataa
     6181 taattcacca gtttcttgaa aaacagaata agaaaaattt atgatatttt ctaggttaaa
     6241 ttcttttata tacggattaa tttgattaaa aaagttttgg tttgaagtat gtttctgatc
     6301 aaaaaataat ccaccatcac tagtcaatat atttccccaa aataatgatt ctgttatttc
     6361 tggattaata actctaacta tgggatcagt tattgtatag ttatcttttg cctttccata
     6421 taaggtatta aaactaaaat acttttggtt attattaaca aatattagtt ttatttttaa
     6481 atttttcaaa ttaggagttt tataaggagc ttgaggagga gagtcttcaa gtctaaactg
     6541 tatgtcttct aaataatttt tttcaatcat ttttgcatat tctttatatt ttttaggtac
     6601 tagaatgtaa gtagttagtg gatcagagtt tattaaattt aagtcagtag attcttcaaa
     6661 agaaatagga ttgtaatatt ttagatagtt taaatcaaca catattcctg caccagcagg
     6721 ttgactaaca tagtcttcaa aatttggata aactttttgt ccaataaaaa attctttatt
     6781 attttcttgg atagttgcgt aattgtatgg cgcgataata aaagttttat aattcttctg
     6841 tattttataa acaaatttac tagctttttt caagtagtta ttttcttcta cggtgttgtt
     6901 tctatttaat tgattagtaa tatttgtttt atataaatta gtagttcttt cccaatattt
     6961 aagagaatta ttttcggctt cattatggat atttattact tttgtagaag ctattaacag
     7021 aaggatcatg cttaagaata gacagatatt taatatacaa agatttaact taagtggtcg
     7081 tgaatttttt agataatttt ttatactaga taaatgatag atacttagag taactatgat
     7141 aaataaaaaa ctaattataa aatgaaaaat actacataga agaaatgata ggaaaaaatt
     7201 ttcgttactg taatatatgc tcaaaaaaat agataaaatg aaagttataa tcaaagaaca
     7261 aaataagagg tttaactctt taatagaatc ttttatagta tcaaaagagt tatagcctaa
     7321 catttttttt agagtgattt ctttcatttt tgaaaaagca aatataattg tagtaattac
     7381 aataaatata ctaagatagc agataacaag aacaatatta gtattaattt gaaaagtatc
     7441 tgattgattg atattggaaa tagtaggtgt tccatatttt ttcagtacag gaattaattt
     7501 gtttgagttt ccttgtacat ataatatttc acttaatcca atattttcca catgagaaaa
     7561 aggataaata tttatattta aaaaattatt tggaagtttt aattggtact tagaattttt
     7621 gttattagta taatggactt tagaatcgtt tcttgctaat atattttttt tagaaaattt
     7681 ccagtgtttg ttagcgttag ggtttgtagc aagaatagtt agttggttaa cagcagtaaa
     7741 actatattga gaaatattaa tattttcttg tgtagatatt tcatacaaag cgtttatata
     7801 actgttaaag tccatatcat ctttatattg catatgaatt tcagcggtat ttttatatag
     7861 cgcttcagaa gtctttatac catttataac attagtagaa aagaatagta ttgtaatgca
     7921 gacaaaaagt gataaaacag cgtttatttt ttttaacata ttttgaattt cctttcaaat
     7981 gtaaataaaa aagagagttt aaactctctt ttttaataaa cattccacca accagtattt
     8041 ccagtaagtg tggcattgca tgttgcataa gctcttactc cagctttctg aactgatcgt
     8101 acttgaccgc ctcgtccatt tttagctgtt gcactatgtt tagctctagc atggtagtaa
     8161 tttgcataaa ctttaaatcc attaacccca cagttgaaat ctccgccgcc agcagcatat
     8221 ctcaatttac tagaagaaaa tccttcttct gtagcttcca ttcctcctcc gtaaatggcg
     8281 gtatggtctt cgggtacatc tagatttaaa gcttctgtag cactaactat tcctccacta
     8341 gttaataaga cacccattaa agcagaaaga cataaaaatt ttttcataac ttatttctcc
     8401 tatctttttt actagaatta ttttatttta gtgtgtttta taaaataaag aacttttttg
     8461 gcgcgttagt atttttatga taaaatttta tgtaaataat cgtttacata ggagaaaaaa
     8521 tggaggattt aggatctaag ctaagagaat taagagtgaa aaaagggtat actcaagaat
     8581 atgtggcaag aaaatttaat gttacacgtc aaactatttc aaagtgggaa aataacaaaa
     8641 caacaccaga tatagagaca ctaaaaaaaa tttgtgaatt ttatagtact aacataaatt
     8701 ctattttaaa tgaagataat ttaaaaaata aacaaaataa tttactggta ttgacagcta
     8761 ttatttttaa tatatttgtt actatattag gtttatttga attgattcaa atgaattttt
     8821 ttagctttac acacggaata tttacttggt ttgctctatc tggattaata gtaatagata
     8881 tcttaaatta tttttatatt tactattctt atttaaaaaa tgaaaaattt ttttatacaa
     8941 tattttttcc cctagtgatt ttgattattt tattaagcta atatgaagta gttagttatt
     9001 taattgcata gttgacataa tgtacagtat gctaagtagc gcctgtatca atcgatacag
     9061 gggctcactt tgtgattttt aattggtcta ggtcaaaagc tctttttaag gcttgatttt
     9121 ctgcagttgc ttctttccgt ttctttgtga atttttacca gtcatttttt actgggttgt
     9181 tatttttcat aaaatatggt aaatttttgg tagcgcttta taaacgcgtt gctttttcaa
     9241 gaataaactc tcattatttt tttaactggc tgctcgaaag agcagtcttt tttattattt
     9301 atccgtttct tttacaaaca attcttcgtt aggactgttg taataatttg gattcaattt
     9361 caattcacca tgtttattcc ctttattgat cactgacaat aacatcggac tgaagcctag
     9421 aattcctagt aaaatagaac cgaatagaag attttttctt gtcatgaata ttccctccag
     9481 ttatttccaa agttgccacc atttttg
//
