LOCUS       NZ_AP024813             2278 bp    DNA     circular CON 21-APR-2024
DEFINITION  Acinetobacter baumannii strain OCU Ac18 plasmid pOCUAc18-11,
            complete sequence.
ACCESSION   NZ_AP024813
VERSION     NZ_AP024813.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00330123
            Sequence Read Archive: DRR301936, DRR301937
            Assembly: GCF_019703285.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1
  AUTHORS   Sakiyama,A., Matsumoto,Y., Tsubouchi,T., Suzuki,M., Niki,M.,
            Niki,M., Oinuma,K.I., Kakeya,H. and Kaneko,Y.
  TITLE     Complete Genome Sequence of a Clinical Isolate of Acinetobacter
            baumannii Harboring 11 Plasmids
  JOURNAL   Microbiol Resour Announc 10 (39), e0069521 (2021)
   PUBMED   34591663
  REMARK    DOI:10.1128/MRA.00695-21
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Tsubouchi,T., Sakiyama,A., Matsumoto,Y., Suzuki,M. and Kaneko,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUL-2021) Contact:Taishi Tsubouchi Osaka City
            University, Bacteriology; Abeno, Asahimachi 1-4-3, Osaka, Osaka
            545-0051, Japan URL :http://www.med.osaka-cu.ac.jp/bacteriology
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Illumina Novaseq; ONT Flongle
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019703285.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 04:56:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,865
            Genes (coding)                    :: 3,655
            CDSs (with protein)               :: 3,655
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 210
            CDSs (without protein)            :: 210
            Pseudo Genes (ambiguous residues) :: 0 of 210
            Pseudo Genes (frameshifted)       :: 58 of 210
            Pseudo Genes (incomplete)         :: 134 of 210
            Pseudo Genes (internal stop)      :: 38 of 210
            Pseudo Genes (multiple problems)  :: 17 of 210
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP024813.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="OCU Ac18"
                     /isolation_source="venous blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="pOCUAc18-11"
                     /geo_loc_name="Japan"
                     /lat_lon="34.68639 N 135.52 E"
                     /collection_date="2016-07-16"
     gene            1..933
                     /gene="repM"
                     /locus_tag="OCUAc18_RS19740"
                     /old_locus_tag="OCUAc18_38690"
     CDS             1..933
                     /gene="repM"
                     /locus_tag="OCUAc18_RS19740"
                     /old_locus_tag="OCUAc18_38690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005265321.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_000711927.1"
                     /translation="MKKELVVKDNALINASYNLDLSEQRLILLAILEARQSNTPNDKDL
                     TIHAESYINHFNVHRNTAYKVLKDACKSLFDRRFSYQKLTQKGNIENVISRWVQRISYV
                     ENEALVRIKFSDDVVPLITNLEKHFTSYELEQVSSLTSVYAIRLYELLIAWRSTGKVTM
                     VELEELRLKLGIEPNEYKRMGQFKEKVLHLAIDQINKYTDIKAEYEQHKRGRSIIGFSF
                     KFKQKQQPKKLDSKRDPNTPDFFIRMTDAQRHLFGNKLAHDARVQSEYSHLIGTGSYED
                     FAKLLADMLAEEQHFKMFYPLLVEHGYKA"
                     /name="repM"
     Typing          362..933
                     /name="rep_cluster_1280"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgaaaaaag aactagtcgt taaagataat gcactaataa atgccagtta taatttagac
       61 ctatcagaac aacgtttaat actgttagcc atacttgaag ctaggcaatc aaatacaccc
      121 aatgataaag atttgacgat tcatgctgaa agctatatca accatttcaa tgttcataga
      181 aatacagcct ataaagttct taaagatgct tgcaaaagcc tatttgatcg tagatttagc
      241 tatcaaaaac tgactcaaaa gggcaacatt gagaatgtaa taagtcgatg ggtacaacga
      301 atatcttatg tagagaatga agctcttgtt cgtattaagt tttctgatga tgttgtaccg
      361 ttgattacaa atttagaaaa acacttcact agttatgaac tagagcaagt cagcagttta
      421 actagtgttt acgctatacg cttgtatgag ttgcttatcg catggcgtag tacgggcaaa
      481 gtgactatgg tagagctaga agagctaaga ttaaaactag gcatagaacc caatgaatac
      541 aagagaatgg ggcaatttaa agaaaaagtt ttacaccttg ccattgatca aattaataag
      601 tacacagata taaaagcaga gtatgaacaa cacaaacgtg gtcgttcaat tattggtttt
      661 tcatttaagt ttaagcagaa acaacaaccg aaaaaattag attctaagcg tgacccaaac
      721 acacctgact tttttatcag aatgaccgat gcacaacgcc acttatttgg gaacaaacta
      781 gcccatgatg ctcgtgtaca aagcgaatac agccatttaa ttggtactgg aagctatgaa
      841 gactttgcaa aattactggc ggatatgctt gcagaagaac agcattttaa gatgttttac
      901 cctttgcttg tagagcatgg ttacaaagca taaatcttgt cgtattctat caatggtaga
      961 taagtcattt cgaccttcct tatctatgta caagataagg tggcgccacc aatacgccaa
     1021 tattggtgga tagccaccaa cccctctaaa ttgcgtttta agcgatccaa atccaacaag
     1081 ctacattcac atacccactg cgagcaaagc tcgaaatatc gcatcctatg agcaaataga
     1141 gcatgtaatc agggagggtg tgtcttgttt ttgcatttca tcagttcact cgtagacact
     1201 cgctctaaaa atctatctgt gcttttcatt ttggctttag ccaaagaaag ccccgaaggg
     1261 gtgtgaagcg aaagcggaac atcatcgaag ccccagcgga gatgactggg gggagccgac
     1321 aacgtaaaag ggagtggaca cgaactttag acatccccag atgtcgttag ttgttggggg
     1381 gcaagcccac cccgtcaaac tatcccgaag gggagttgac ccacctcgtt ttagtgtaag
     1441 cgttcagcga atccgaagga catacgagcc taagcgaaat atgcacctaa aagggggtgg
     1501 gtcaactccg taggaggatg acggggcgcg agcacgaagt gcgctgcaac ttggagcgat
     1561 agcggaaagt gaaaggcaac aaaaaacgat tattaagggc gtccagccct taaagtacaa
     1621 ggaatcctga cgtccaggaa ggacgagcga agcgagcttg tacggtaatt gtttttaggc
     1681 gcagcgaagc gaagtgtctg tgccttttac tgtgctttta tcggtgcact aaaaatccaa
     1741 taaaagcacc atagaaagca cagatagaat taaccgagcg agtgtctacg agcgattgag
     1801 catcatcaaa atttcgccct cgccttttct ctgattacaa gctccctttc gattttaaac
     1861 ggtaggtaca gagcatcccg aatgggtgcg aactttggag cttttgcttt tcaaaaagta
     1921 tgagcgaagc gaatacatta caaatgcttt tgcttttaga tttcacaaac aggattaact
     1981 aaaaactgcc ccacgaagcg aggaacgagc ttcaatagag tacgagcttt agcgagtaca
     2041 tagggcagtt ttaagcactc ccctcttttt aataattttt aattttttaa tttttagcct
     2101 ctgtaattaa ttgataaata ataataaaaa taaggataag tacaccttta tcttgcaata
     2161 agtacacctt tatcttgcaa taagtacacc tttatcttgc aataagtaca cctttatctt
     2221 gcaaagtgac acttaataaa atagagtgta cttatatact taaaagacat gtgtgctt
//
