LOCUS       NZ_AP024812             2444 bp    DNA     circular CON 21-APR-2024
DEFINITION  Acinetobacter baumannii strain OCU Ac18 plasmid pOCUAc18-10,
            complete sequence.
ACCESSION   NZ_AP024812
VERSION     NZ_AP024812.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00330123
            Sequence Read Archive: DRR301936, DRR301937
            Assembly: GCF_019703285.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1
  AUTHORS   Sakiyama,A., Matsumoto,Y., Tsubouchi,T., Suzuki,M., Niki,M.,
            Niki,M., Oinuma,K.I., Kakeya,H. and Kaneko,Y.
  TITLE     Complete Genome Sequence of a Clinical Isolate of Acinetobacter
            baumannii Harboring 11 Plasmids
  JOURNAL   Microbiol Resour Announc 10 (39), e0069521 (2021)
   PUBMED   34591663
  REMARK    DOI:10.1128/MRA.00695-21
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Tsubouchi,T., Sakiyama,A., Matsumoto,Y., Suzuki,M. and Kaneko,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUL-2021) Contact:Taishi Tsubouchi Osaka City
            University, Bacteriology; Abeno, Asahimachi 1-4-3, Osaka, Osaka
            545-0051, Japan URL :http://www.med.osaka-cu.ac.jp/bacteriology
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Illumina Novaseq; ONT Flongle
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019703285.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 04:56:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,865
            Genes (coding)                    :: 3,655
            CDSs (with protein)               :: 3,655
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 210
            CDSs (without protein)            :: 210
            Pseudo Genes (ambiguous residues) :: 0 of 210
            Pseudo Genes (frameshifted)       :: 58 of 210
            Pseudo Genes (incomplete)         :: 134 of 210
            Pseudo Genes (internal stop)      :: 38 of 210
            Pseudo Genes (multiple problems)  :: 17 of 210
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP024812.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="OCU Ac18"
                     /isolation_source="venous blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="pOCUAc18-10"
                     /geo_loc_name="Japan"
                     /lat_lon="34.68639 N 135.52 E"
                     /collection_date="2016-07-16"
     gene            join(2321..2444,1..1103)
                     /locus_tag="OCUAc18_RS19720"
     CDS             join(2321..2444,1..1103)
                     /locus_tag="OCUAc18_RS19720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001990871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rolling circle replication protein, Rep63
                     protein"
                     /protein_id="WP_226383026.1"
                     /translation="MYGNNDVSQREQASIARGLAVASADVPLGIFTKSQATFKNTGVER
                     LHNFILQDQACKLLPKHRVCNCLKKRIDKTKDRLVKYNVDRKKAHWSNVQRCGCLWIDP
                     VCAKQITEGRRSELSYALERWKKEFLGGCLLLTLTFSHSLDMPLSASLLGLRDAYTRFV
                     GHRRVKEIFKAIGVENKIKGLEILWGQNGWHPHNHILLLTKYTVLGFESYRDELARLWI
                     NSCLKAGLRSPSMEHGLDIRDGSHAEEYISKYGLPDQNKWGLPEEITKGHMKKGRNGGF
                     TPFDLLQLSIEDKPLYGGKLPSKLFQEFAFAIKGSSQLVWGRGLKKLLCIEEKTDQELA
                     EETEKNAIELTSVTNYGFTLLTVFQKRHVFLDCVARDYEQKDGFSSANALLDELHFKYL
                     EIQQSKQLE"
                     /name="rolling circle replication protein, Rep63 protein"
     gene            1196..1549
                     /locus_tag="OCUAc18_RS19725"
                     /old_locus_tag="OCUAc18_38660"
     CDS             1196..1549
                     /locus_tag="OCUAc18_RS19725"
                     /old_locus_tag="OCUAc18_38660"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_221255538.1"
                     /translation="MDLRVFPGRSDNQKFYVLGYSVMNKNDALVSKDGEFFSVPFADAY
                     LFSNSERAQNYIDQNGLKKRGFKVVKTFQYLRAIGYASLEHTDEFIQTEMDKEKDMFLN
                     TINFHFDGLDDEF"
                     /name="hypothetical protein"
     gene            1539..1742
                     /locus_tag="OCUAc18_RS19730"
                     /old_locus_tag="OCUAc18_38670"
     CDS             1539..1742
                     /locus_tag="OCUAc18_RS19730"
                     /old_locus_tag="OCUAc18_38670"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_221255535.1"
                     /translation="MNFKDKLTAKEIEYLLSLVESDYEEVAQYVDSKELEKEDREQYIY
                     YCEQYAMIDNLRKKLDVELAFE"
                     /name="hypothetical protein"
     gene            1958..2242
                     /locus_tag="OCUAc18_RS19735"
                     /old_locus_tag="OCUAc18_38680"
     CDS             1958..2242
                     /locus_tag="OCUAc18_RS19735"
                     /old_locus_tag="OCUAc18_38680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004929869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_221255536.1"
                     /translation="MANQKKRIALTVPDDMDAVLERLSILTNAPKTKLIMEMLEEYMPV
                     LERTVDALEQIQANKANAPEIAKHFASELLLEGTEKLGIIATEAKKLKE"
                     /name="hypothetical protein"
ORIGIN
        1 gtgttgaacg tcttcataat ttcattttac aagaccaagc ctgcaaactt ttgcctaagc
       61 atcgcgtatg taactgctta aaaaaacgca ttgataaaac aaaagatcgt cttgttaaat
      121 acaacgtaga tcgaaaaaaa gcacactggt cgaatgtaca gcgttgtggc tgtctttgga
      181 ttgatcctgt ttgtgctaaa caaattacag aaggaagacg ttcagagctt tcttatgctc
      241 ttgaacgttg gaaaaaggaa tttctaggcg gttgtcttct tcttactctt actttttcgc
      301 attcgcttga tatgcccctt tctgcttctc ttttaggcct tagagatgct tatactcgtt
      361 ttgttggtca tagacgtgtt aaagagattt ttaaggctat tggcgttgaa aataaaatta
      421 aagggttaga aatcctttgg gggcaaaatg gatggcatcc tcacaatcat attttgcttt
      481 taactaaata cactgttcta ggatttgagt cttacagaga tgaattagca cgtctttgga
      541 ttaattcttg tcttaaagct ggtctacgtt caccaagcat ggaacatggt ttagatattc
      601 gtgatggatc acatgctgag gaatacatca gtaagtatgg cttacctgat caaaataaat
      661 gggggcttcc tgaagaaatc actaaaggtc atatgaaaaa aggccgtaac ggtggtttta
      721 ctccttttga ccttttacag ctatctattg aagataaacc gttatacggt ggcaaattgc
      781 cttcaaaatt atttcaagaa tttgcttttg cgattaaagg ttcttctcaa cttgtttggg
      841 gtcgtggtct taaaaaactg ctttgtattg aagaaaaaac agatcaggaa ttagcagaag
      901 aaacagaaaa aaatgcaatt gaattaactt ctgtcactaa ttatggattt actcttttga
      961 ctgttttcca aaaaagacac gtctttttag attgtgttgc tagggattat gaacaaaagg
     1021 atggtttctc cagtgctaac gctcttttgg atgaacttca ttttaaatat ttagaaatcc
     1081 aacaatccaa acaacttgaa tagttgcaag gacgacaatg acgaggagga gctgcgacaa
     1141 cgaggaagcg gaggacgcag caaaaataca agttttatta ttatgaggtt ttattatgga
     1201 tttacgtgtt ttccctggac gcagtgataa tcaaaaattt tacgtcttag gctattcagt
     1261 tatgaataaa aatgacgctc ttgtttctaa ggatggagag tttttttctg ttccttttgc
     1321 agatgcttat ttattttcaa actctgaacg tgctcagaat tacatagatc aaaatggtct
     1381 taagaagcgt ggttttaaag ttgttaaaac tttccaatac cttagagcta ttggttatgc
     1441 gtcacttgaa catacagatg agtttattca aacagaaatg gataaagaaa aagatatgtt
     1501 cttgaacact attaactttc actttgatgg gcttgatgat gaattttaaa gacaagctaa
     1561 cagctaaaga aattgaatat ttacttagtc tggtcgaatc tgattatgaa gaagttgctc
     1621 aatatgtcga ttctaaagaa ttggaaaaag aagacagaga acaatacatc tactactgtg
     1681 aacaatacgc catgatcgac aatctacgca aaaaattgga tgtcgaacta gcttttgaat
     1741 aaaagtgtag tgagtacacc tttaccgaac gagtgtctac gagtgacgac tccgtaataa
     1801 aaattacaga gccttccctc tctgattaca agctcccctt agcttctaag cgggagtttt
     1861 tagtttttgg gttggggttc ccaaaaacgt cacgaaaaca tttgcttatt tttaaattaa
     1921 tgttacttta tataaaaata gttacttggt gaaattcatg gccaatcaga aaaaacgaat
     1981 tgctttaact gtccctgatg atatggatgc tgttttagag cgtttgtcca ttttaactaa
     2041 tgcaccaaag actaaattaa ttatggaaat gcttgaggaa tacatgcctg ttcttgagcg
     2101 tactgtagat gctttagagc agattcaagc taataaggct aatgcaccag agatagcaaa
     2161 acactttgct agtgaattgt tattagaagg gaccgagaaa ttaggaatta ttgcaactga
     2221 ggcaaaaaaa cttaaggaat aataataaag ctctagtgac tggtactcac tagagctttg
     2281 gaatcctgta gttgtacggc aacatttagg agtaactcta ttgtacggta acaatgatgt
     2341 aagtcaaagg gaacaggcga gcattgcgcg agggctcgca gtcgcaagcg cagatgttcc
     2401 tctaggtata tttacgaaat ctcaggccac ttttaaaaat acgg
//
