LOCUS       NZ_AP024811             3496 bp    DNA     circular CON 21-APR-2024
DEFINITION  Acinetobacter baumannii strain OCU Ac18 plasmid pOCUAc18-9, complete
            sequence.
ACCESSION   NZ_AP024811
VERSION     NZ_AP024811.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00330123
            Sequence Read Archive: DRR301936, DRR301937
            Assembly: GCF_019703285.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1
  AUTHORS   Sakiyama,A., Matsumoto,Y., Tsubouchi,T., Suzuki,M., Niki,M.,
            Niki,M., Oinuma,K.I., Kakeya,H. and Kaneko,Y.
  TITLE     Complete Genome Sequence of a Clinical Isolate of Acinetobacter
            baumannii Harboring 11 Plasmids
  JOURNAL   Microbiol Resour Announc 10 (39), e0069521 (2021)
   PUBMED   34591663
  REMARK    DOI:10.1128/MRA.00695-21
REFERENCE   2  (bases 1 to 3496)
  AUTHORS   Tsubouchi,T., Sakiyama,A., Matsumoto,Y., Suzuki,M. and Kaneko,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUL-2021) Contact:Taishi Tsubouchi Osaka City
            University, Bacteriology; Abeno, Asahimachi 1-4-3, Osaka, Osaka
            545-0051, Japan URL :http://www.med.osaka-cu.ac.jp/bacteriology
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Illumina Novaseq; ONT Flongle
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019703285.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 04:56:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,865
            Genes (coding)                    :: 3,655
            CDSs (with protein)               :: 3,655
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 210
            CDSs (without protein)            :: 210
            Pseudo Genes (ambiguous residues) :: 0 of 210
            Pseudo Genes (frameshifted)       :: 58 of 210
            Pseudo Genes (incomplete)         :: 134 of 210
            Pseudo Genes (internal stop)      :: 38 of 210
            Pseudo Genes (multiple problems)  :: 17 of 210
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP024811.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3496
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="OCU Ac18"
                     /isolation_source="venous blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="pOCUAc18-9"
                     /geo_loc_name="Japan"
                     /lat_lon="34.68639 N 135.52 E"
                     /collection_date="2016-07-16"
     gene            13..1335
                     /locus_tag="OCUAc18_RS19710"
                     /old_locus_tag="OCUAc18_38640"
     CDS             13..1335
                     /locus_tag="OCUAc18_RS19710"
                     /old_locus_tag="OCUAc18_38640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942531.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_057070934.1"
                     /translation="MLNNLNDQNLPSSTNSALASFGGVTAFKKSRLGTIVKLCSTVDSK
                     EKNLALEKAREYRKRNIKLKDRAGEILYIPNQKKQAPVCSCGKCRIDGSAPVGVDYNQK
                     TGMASYTNLQYCGSVWVCPDCSYKISQERKKELAEAMKGCVDKGLHVAMLTLTVPHYLG
                     DDLRALLKKMSKAKHSLWSNRNSREYIADQFPMIGHITATEVKYSDSNGFHPHYHILCF
                     LDKQYQKEDLEIIENELYEFWAEKCVKAGLGKPNRRNGIDLKMGSNNADTLADYIAKWG
                     IAEEMTQAHLKIGKNNLQSLTMWEVLELAQMEASTTDKYSHIFKTYAVAFKGRKQLHWS
                     KGLKKLLECPVKDDEEIANTNTEEDPTEVVHALFLSPQDFWAICYHKKRAEFLELVESD
                     FAKNGIQTDLKSVRDFLIRLKNARRFSGGQGGNLPLCERTI"
                     /name="protein rep"
     gene            1434..3128
                     /gene="mobV"
                     /locus_tag="OCUAc18_RS19715"
                     /old_locus_tag="OCUAc18_38650"
     CDS             1434..3128
                     /gene="mobV"
                     /locus_tag="OCUAc18_RS19715"
                     /old_locus_tag="OCUAc18_38650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016653420.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_151813242.1"
                     /translation="MSYAILRTEKLKTMGNIAASLSHNYRNRPTPNADPYRTVNNEHDL
                     KTAGQVMDGIKNRLPEKTRSNAVLCVEYLITMSPDWSGLGTEKEADFFKRSLEWLKQRH
                     GAENVISTSIHRDETTPHLVAYVVPIDLEGKLNAREFLGGRSKLSKMQTDFYNEVKHLG
                     LERGLEGSKAEHTTIKDFYAEIQKPDNEKYLIQKPPLKTITYNDSAIHMLDGKPLKDEK
                     LQKYFGSLFDEQHEYYQKQSLKAQEALSVKLSEQIIKTEKEAEAHRNAVKQIKKLEKKI
                     DKMLDEFSQIIEFKELFPNDYKEIEQSLKDRIQDHKNQLIRKREEDARFEAQLRAQREQ
                     LAKEQERQQAIEFRKTLESDRKMQIEADRAHLRDKVGECTTEPEKLAYSAIQRKLEGVI
                     REGANYTVLLDNNEPTTNPYAEILRLMGDREVYDFKKKLEIILNQCADSKSKDYDDTFR
                     SWGTRPSYYDNFDSSAKYIVAIQSIIDDQVQEGGVLAYEKANEVKRALSDVLVGKCKNE
                     YDVLLIDEKSKERQRQYNESLELSNKNQLDNSLYFEEEKKKGNDFEM"
                     /name="mobV"
ORIGIN
        1 atgggcttga gcatgctaaa caatctaaac gatcaaaacc taccttcctc tactaacagc
       61 gctctagcgt catttggtgg cgttactgca ttcaaaaaaa gtcgccttgg gacaatcgtc
      121 aaattatgtt ccaccgttga ttctaaagag aaaaatttag ctcttgaaaa ggctcgtgaa
      181 tatagaaaac gaaatattaa gctaaaagat agagcagggg agattctcta tatcccaaac
      241 caaaagaaac aagctccagt atgtagctgt gggaaatgtc gtattgatgg cagcgcccct
      301 gtaggtgtgg attacaacca aaaaacagga atggctagtt atacaaattt gcagtattgc
      361 ggttctgttt gggtgtgtcc tgactgtagt tataaaatta gccaggaacg gaaaaaagaa
      421 ttagccgaag cgatgaaagg ctgtgttgat aagggtttgc atgtggcaat gcttacattg
      481 actgtgcctc actatcttgg tgatgacctc cgagcattgc ttaaaaaaat gagtaaggcg
      541 aagcattctt tatggtcgaa tagaaatagc cgtgaataca ttgcagacca attcccaatg
      601 attgggcata tcacagctac agaagtgaaa tatagtgata gcaatggttt ccacccccac
      661 taccatatcc tgtgtttttt agataagcag tatcaaaaag aagatttaga gattatcgaa
      721 aatgaacttt atgagttctg ggctgagaag tgtgtcaaag ctggacttgg taagcctaat
      781 cgtaggaatg gtatagattt aaaaatgggt tctaacaatg ctgataccct tgctgattat
      841 attgctaagt ggggaattgc agaggaaatg actcaagcac atttgaagat tggtaaaaat
      901 aatttgcagt ctttaacgat gtgggaagtc cttgagcttg cccaaatgga agcaagtaca
      961 acagataaat attcacatat atttaaaacc tatgccgttg ctttcaaagg aagaaagcaa
     1021 cttcattggt caaagggttt aaagaaatta ttagaatgtc cggttaagga tgacgaggaa
     1081 attgcaaata caaatactga agaagatccg actgaagttg ttcatgctct ttttcttagt
     1141 cctcaagatt tttgggcaat ctgttatcac aaaaaacgtg ccgagtttct tgaactcgtt
     1201 gaatccgatt ttgctaaaaa tgggattcag accgatctaa aatccgtaag ggattttttg
     1261 atcaggctta aaaacgcaag gcgtttttca gggggtcaag ggggaaatct ccccctctgc
     1321 gagcggacaa tttgaaaacg tagttttaaa attgtgtagt gagtacacat tccgcttcgc
     1381 taatgttttt ccctgtggca cactggggga aaactagcga taggatgtgt tttatgagtt
     1441 atgcaattct cagaacagaa aaactgaaaa cgatgggcaa tattgctgca tctttgagcc
     1501 ataactatcg caatagacct actcctaatg ctgacccata ccgcactgtt aataatgaac
     1561 atgatttaaa aacagcagga caggtgatgg atgggattaa aaatcgactt cctgaaaaaa
     1621 cacgttcaaa cgctgtccta tgtgttgaat atttaataac gatgtctcca gattggtcgg
     1681 gattggggac agaaaaagaa gcggattttt ttaaacggtc tctggaatgg ttaaaacaaa
     1741 ggcatggtgc tgaaaatgtt attagtacct cgatccaccg tgatgaaact accccgcacc
     1801 ttgttgcata cgttgttcct attgatttag aggggaaatt gaacgctagg gaatttttag
     1861 gcggtaggtc aaaattatca aaaatgcaga ctgattttta taatgaggtt aagcatttag
     1921 gacttgaaag ggggttagag gggagcaagg cagaacatac gacaattaaa gatttttatg
     1981 cagaaattca aaagcctgat aatgaaaaat atttaattca aaaaccacct ttaaaaacaa
     2041 ttacttataa tgattctgca attcatatgc tagatggaaa acctttaaaa gatgagaagc
     2101 ttcaaaaata ttttggaagt ctttttgatg agcagcatga atactatcaa aagcagtctt
     2161 taaaagccca agaagcccta tctgtaaagc tttcagagca aattattaag acagagaaag
     2221 aagcagaagc ccatagaaat gcagtaaaac agataaaaaa attagaaaaa aagattgata
     2281 aaatgcttga tgaattttct caaattattg agtttaaaga actttttcca aatgactaca
     2341 aggaaataga acaaagttta aaagatcgaa tacaagatca taaaaatcaa ttgattagaa
     2401 aaagggaaga ggatgcacga tttgaagcgc aattaagggc gcaacgtgaa cagttagcaa
     2461 aagagcaaga gaggcaacag gcaatagaat ttaggaagac actagaaagt gatcgtaaaa
     2521 tgcaaataga ggctgataga gcgcatttac gagataaagt tggtgaatgt actactgaac
     2581 ctgaaaaact ggcttatagc gcaatacagc gcaaattaga gggtgttatt agagaggggg
     2641 ctaactacac tgttttgctt gataataatg agcctacaac taacccatat gccgagatac
     2701 ttaggctaat gggtgataga gaagtatatg attttaaaaa gaaattagaa attatattaa
     2761 atcaatgtgc agattctaag agtaaagatt atgacgatac ttttagaagt tggggaacaa
     2821 gaccaagtta ctatgataat tttgattcat ctgcaaaata tattgttgca attcaaagca
     2881 ttatagatga tcaagtgcaa gaggggggtg tattggctta tgaaaaagcg aatgaagtaa
     2941 aacgtgcttt aagtgatgta ttggttggta agtgtaaaaa tgaatatgac gtacttttga
     3001 ttgatgagaa gtcgaaagaa aggcagcgtc aatacaatga atctcttgaa ttatctaata
     3061 aaaatcaatt agataatagt ttgtattttg aagaggaaaa gaaaaagggc aatgattttg
     3121 agatgtagac tttagcgagt gtctacgagc gtcacagtag tagtaaaatt catgatgttt
     3181 ttctctctga ttacctactc ccttttgatc gcaaacggga gtaacagagc atcccaacgg
     3241 gtgcgaacaa cttagaaaac aatggcttag atatttaact agagtaaata tttagtgtaa
     3301 tttttacttt aaaatcagat atttgaataa cagaaatatt taagttatat tacttttagt
     3361 tttttacaga ccaaaaaaaa gccccatatc ttggcggatc aggcttttga ttggctactt
     3421 tcagcggctt gaggtgctga tttcacaatg tatacggaca gtataaaggt gatttccctc
     3481 tccatcaagc ctgatt
//
