LOCUS       NZ_AP024810             3948 bp    DNA     circular CON 21-APR-2024
DEFINITION  Acinetobacter baumannii strain OCU Ac18 plasmid pOCUAc18-8, complete
            sequence.
ACCESSION   NZ_AP024810
VERSION     NZ_AP024810.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00330123
            Sequence Read Archive: DRR301936, DRR301937
            Assembly: GCF_019703285.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1
  AUTHORS   Sakiyama,A., Matsumoto,Y., Tsubouchi,T., Suzuki,M., Niki,M.,
            Niki,M., Oinuma,K.I., Kakeya,H. and Kaneko,Y.
  TITLE     Complete Genome Sequence of a Clinical Isolate of Acinetobacter
            baumannii Harboring 11 Plasmids
  JOURNAL   Microbiol Resour Announc 10 (39), e0069521 (2021)
   PUBMED   34591663
  REMARK    DOI:10.1128/MRA.00695-21
REFERENCE   2  (bases 1 to 3948)
  AUTHORS   Tsubouchi,T., Sakiyama,A., Matsumoto,Y., Suzuki,M. and Kaneko,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUL-2021) Contact:Taishi Tsubouchi Osaka City
            University, Bacteriology; Abeno, Asahimachi 1-4-3, Osaka, Osaka
            545-0051, Japan URL :http://www.med.osaka-cu.ac.jp/bacteriology
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Illumina Novaseq; ONT Flongle
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019703285.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 04:56:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,865
            Genes (coding)                    :: 3,655
            CDSs (with protein)               :: 3,655
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 210
            CDSs (without protein)            :: 210
            Pseudo Genes (ambiguous residues) :: 0 of 210
            Pseudo Genes (frameshifted)       :: 58 of 210
            Pseudo Genes (incomplete)         :: 134 of 210
            Pseudo Genes (internal stop)      :: 38 of 210
            Pseudo Genes (multiple problems)  :: 17 of 210
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP024810.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3948
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="OCU Ac18"
                     /isolation_source="venous blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="pOCUAc18-8"
                     /geo_loc_name="Japan"
                     /lat_lon="34.68639 N 135.52 E"
                     /collection_date="2016-07-16"
     gene            1..1011
                     /gene="repM"
                     /locus_tag="OCUAc18_RS19695"
                     /old_locus_tag="OCUAc18_38610"
     CDS             1..1011
                     /gene="repM"
                     /locus_tag="OCUAc18_RS19695"
                     /old_locus_tag="OCUAc18_38610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846161.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_221255533.1"
                     /translation="MRTELVVKDNALINASYNLDLVEQRLILLAIVEARESGRGISTNK
                     PLVVHASSYINQFDVARQTAYQALKNACKDLFARQFSYQEKREKGVANITSRWVSQIAY
                     IDDTATVELIFSPAIIPLITRLEEQFTSYELTQISGLTSAYAVRLYELLIAWRSTGKTP
                     VIELSEFRAKMGVLDGEYTRSDNFKKWVIEKPIEQINEHTDITVKVEQHKTGRSITGFS
                     FRFKQKQQPKIEKPIDQKRDPKTVDMFTGKTDAETKNIARAVVKDSSQPANTLQHRASR
                     ITNAIISNKLHERFKHGDEGISSTLTRIQAEIISGGNETADYWQSKLEDFGITFD"
                     /name="repM"
     gene            complement(1037..1342)
                     /locus_tag="OCUAc18_RS19700"
                     /old_locus_tag="OCUAc18_38620"
     CDS             complement(1037..1342)
                     /locus_tag="OCUAc18_RS19700"
                     /old_locus_tag="OCUAc18_38620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005320422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WP_221255534.1"
                     /translation="MTDEKEPKPKLTLEQKIEQQEQKLKQLKAQKQAAEAREKARKKEQ
                     DRKDDTRIKILLGSYLKKKMDNDAEFNSKTLDELENYLTANRDRALFGLSPLDANL"
                     /name="mobilization protein"
     gene            1567..3147
                     /gene="mobQ"
                     /locus_tag="OCUAc18_RS19885"
                     /old_locus_tag="OCUAc18_38630"
     CDS             1567..3147
                     /gene="mobQ"
                     /locus_tag="OCUAc18_RS19885"
                     /old_locus_tag="OCUAc18_38630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_226383025.1"
                     /translation="MAIYHFSVKAISRSDGRSAVACAAYRAAEKLVCDYYGKEQDYTKK
                     TGVEFTNIYAPENTKKELLDRQKLWNAVEKVENRKNSLLAREFEIAFPSELNAEQRKTL
                     LDELCQELVKKHGVIVDAAIHAPHIEGGSDDRNYHAHIMFTTRSISKTGDFEAKKFRDF
                     SRDNGTETVKAWRETFADLVNVQLEAIGSKERVSHLSYEELGNGLEATVHEGYAVTQLR
                     RLGIDTEISLENDAIKQRNAEKTVNEQAITGLDQEIIASESILSDLQRSKAKSDAEHAQ
                     QQQIALERKRERLEAQSLADAHKRATDFHTAFDPNHPDKSLVTQYITDYALLARHGKQP
                     QPPAPVEQPQPTWWQRMTGQQPQQIEQPPFFEIEVVKATAQAIVEHEKKQAQQQKWQRL
                     QEFLQYSPSTVDRLERDAISRSFDKGQQAGDEELAEFIKYHRQHWREKEQAAKDWQAER
                     DRFMREEAAKAREEAALTLQRERESEAKLEAYRERKALLDKEIAEMQQKPVNRDEIQQQ
                     PRPKNDFEP"
                     /name="mobQ"
ORIGIN
        1 atgagaacag aattagtcgt gaaagacaat gccctaatca atgctagtta taaccttgat
       61 ctagtcgagc aacggttaat tcttctagct atcgttgaag caagggaatc ggggcgtgga
      121 attagtacaa ataagcctct tgtagtccat gcatctagct atatcaatca atttgatgta
      181 gctagacaaa cagcctatca agctttgaaa aatgcctgca aggacttgtt tgcacgtcaa
      241 tttagctacc aagaaaaacg agaaaaagga gtcgcaaaca taactagcag gtgggtgtca
      301 caaatcgcat atattgacga tacagctacc gttgaattga tcttctcacc tgcaatcatt
      361 ccattgatca caagacttga agaacagttc acaagctatg aactgacaca aattagcggt
      421 ttaaccagtg cctatgctgt ccgattatat gaactgctga ttgcatggcg cagcactggt
      481 aaaacgcctg tgatcgaatt atcagagttt cgggcaaaaa tgggagttct agacggtgaa
      541 tacactcgat ctgacaattt taaaaagtgg gttatagaaa aaccgattga acagattaac
      601 gagcatacag acatcacggt caaggttgaa caacataaaa cagggcgttc gatcactggt
      661 ttttctttta gattcaagca aaaacaacaa ccaaaaattg aaaaaccgat tgatcagaag
      721 cgagatccaa agaccgttga tatgttcaca ggtaaaactg atgctgagac aaaaaacata
      781 gctagagcag ttgttaaaga cagctcacag cctgcaaata cattgcagca tcgagctagt
      841 cgtattacca acgcaatcat aagcaataag ctacatgaac gctttaaaca tggggacgag
      901 ggtatatcta gcaccttaac acgtattcaa gccgagatta ttagcggtgg taatgaaacg
      961 gcagactact ggcaatcgaa acttgaggat tttgggataa cttttgatta aaaaatacac
     1021 atgatttttg taccgtttaa aggttagcat caagagggga tagcccgaat aaagctcgat
     1081 ctcgattagc ggtcaggtag ttttctagct catcaagtgt ttttgaattg aactctgcat
     1141 cattatccat ttttttcttc agataagaac cgagcaaaat ttttatacgt gtatcgtctt
     1201 ttctatcttg ctctttttta cgtgcttttt ctcttgcttc agcagcttgt ttttgtgcct
     1261 ttaactgctt tagtttctgc tcttgctgtt caattttctg ttctaaagtc agctttggct
     1321 ttggctcttt ttcgtctgtc attctctttt ccaatattta ggattcttct cgatcataag
     1381 cgcatttgta ctgtcgtaca agatgctata tgatactcaa agaatccccg aagggcgcac
     1441 ttagccaaac taaatccata ctttcagtat gtgattagtt tggtgcgaaa ggggactccc
     1501 ctttacccct gaccaacctg ttcaggttgg taatttcgat aaatcgaaat aaagagcaaa
     1561 agcaaaatgg cgatatatca tttttcagtt aaagcgatta gtcgtagtga tggacgtagt
     1621 gccgttgctt gtgctgcata tcgcgcggct gaaaaattgg tatgcgatta ctacggaaaa
     1681 gagcaagact acacaaaaaa aacaggtgtc gaatttacaa atatctatgc acctgaaaac
     1741 acaaaaaaag aattattaga ccgtcaaaaa ttgtggaacg cagtcgagaa agtcgagaac
     1801 agaaaaaatt cacttcttgc ccgtgaattt gaaattgcct tcccatccga actaaacgca
     1861 gaacagagaa aaaccctact tgatgaactg tgccaggaac ttgtcaaaaa gcatggcgtg
     1921 atcgttgatg cagcgataca tgctccacat attgaaggtg gatctgatga ccgcaactat
     1981 catgctcaca tcatgtttac gacccgatct attagtaaaa ctggtgactt tgaagccaaa
     2041 aaattcaggg acttcagcag agacaatggg acagaaacag tcaaagcatg gcgtgaaaca
     2101 tttgcggatc tagtgaatgt tcaacttgaa gctattggct ctaaagagcg tgttagccac
     2161 ttgagctacg aagagctagg caacgggcta gaagccaccg tacatgaggg ctacgcagtc
     2221 acacagctac gcagacttgg cattgataca gaaatcagcc ttgaaaatga tgctatcaaa
     2281 cagcgtaacg ctgaaaaaac tgtaaatgaa caagcgatca ctggactaga tcaggaaatc
     2341 atagcgtcag agtcgatttt aagcgattta cagcgatcta aagccaaaag tgatgcagaa
     2401 catgctcaac aacaacaaat cgctttagaa cgcaaaagag agcgtttaga ggcacaatct
     2461 ctagctgatg cacataaacg agcgacagat tttcatacag cgttcgaccc caatcaccct
     2521 gacaagtctt tagtaaccca gtacatcact gattacgcct tgcttgcccg acatggcaag
     2581 caaccgcaac cccctgcccc agtagaacaa ccgcagccga catggtggca acggatgacg
     2641 gggcaacagc cacaacaaat tgaacaaccg ccattttttg aaatcgaggt ggtcaaagcc
     2701 actgcccaag ctattgttga gcatgagaaa aaacaggcac aacagcagaa atggcaacgg
     2761 ttacaagagt ttttacaata ctctcctagc actgtagaca gacttgagcg agatgctatc
     2821 agcagatcgt ttgacaaggg acagcaagca ggtgatgaag aacttgccga gttcatcaaa
     2881 taccaccgcc aacattggcg ggaaaaagag caggcagcta aagactggca agccgagcgt
     2941 gaccgcttta tgcgtgagga agcagctaaa gcaagggaag aagctgcact cacactacaa
     3001 agagagcgtg agtcagaagc caaacttgaa gcgtacagag agcgcaaagc attgcttgac
     3061 aaggaaatag ctgaaatgca gcaaaagcct gttaataggg atgagataca acaacagcca
     3121 cgccctaaaa atgactttga gccatagatt atttattttt tctaaataat aagaacagtt
     3181 tacagagcga gtgtctacga gcgaacaccc aaatttcgcc ccttgccctc tgaaaaaccg
     3241 ctttttcgcc ttttgaccct agaaagtgcc tatttttgtg tgctgatttt gatgtatccc
     3301 attttattaa cagtctataa cccttgcagc tattagcttt cgtgtgcttt tgctcttaaa
     3361 aaagccttga gcgatagcga aagccatagt cgcttttgct tttgcctttg cttttcaaaa
     3421 agacacgagc gaagcgagtg ccatagcctt tgattttgac cttgcttttg cctttgattc
     3481 ccttttttag tttttaagaa gcttttaaaa gcttttagcc ccccctatag cccacgtata
     3541 tcaatgcttt cagactttaa aaaagtacaa acgccaagta ttaaagtaca tattttaaaa
     3601 atgtacttca ttgctgcttt tgcttttaga tttcacagat aaagttaact taaaaattgc
     3661 ccctagttgc gaggaacgag caacaataga gtacgagctt tagcgagtac ctagggcaat
     3721 ttcatgatgg tgcttgggct tttaataata aaaaagcttt taaatgcttt tagctcgcct
     3781 gaaaaccttt attagcaatg ctttcagaga tcattatcct aggttttcct accaatactc
     3841 ctaggttttc ctaccattat aacagtaata ctcctaggtt taccttgcat tatcctagta
     3901 aatatactaa aaagtaatta ctagttataa ataatcacta ggattaaa
//
