LOCUS       NZ_AP024809             4293 bp    DNA     circular CON 21-APR-2024
DEFINITION  Acinetobacter baumannii strain OCU Ac18 plasmid pOCUAc18-7, complete
            sequence.
ACCESSION   NZ_AP024809
VERSION     NZ_AP024809.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00330123
            Sequence Read Archive: DRR301936, DRR301937
            Assembly: GCF_019703285.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1
  AUTHORS   Sakiyama,A., Matsumoto,Y., Tsubouchi,T., Suzuki,M., Niki,M.,
            Niki,M., Oinuma,K.I., Kakeya,H. and Kaneko,Y.
  TITLE     Complete Genome Sequence of a Clinical Isolate of Acinetobacter
            baumannii Harboring 11 Plasmids
  JOURNAL   Microbiol Resour Announc 10 (39), e0069521 (2021)
   PUBMED   34591663
  REMARK    DOI:10.1128/MRA.00695-21
REFERENCE   2  (bases 1 to 4293)
  AUTHORS   Tsubouchi,T., Sakiyama,A., Matsumoto,Y., Suzuki,M. and Kaneko,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUL-2021) Contact:Taishi Tsubouchi Osaka City
            University, Bacteriology; Abeno, Asahimachi 1-4-3, Osaka, Osaka
            545-0051, Japan URL :http://www.med.osaka-cu.ac.jp/bacteriology
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Illumina Novaseq; ONT Flongle
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019703285.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 04:56:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,865
            Genes (coding)                    :: 3,655
            CDSs (with protein)               :: 3,655
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 210
            CDSs (without protein)            :: 210
            Pseudo Genes (ambiguous residues) :: 0 of 210
            Pseudo Genes (frameshifted)       :: 58 of 210
            Pseudo Genes (incomplete)         :: 134 of 210
            Pseudo Genes (internal stop)      :: 38 of 210
            Pseudo Genes (multiple problems)  :: 17 of 210
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP024809.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4293
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="OCU Ac18"
                     /isolation_source="venous blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="pOCUAc18-7"
                     /geo_loc_name="Japan"
                     /lat_lon="34.68639 N 135.52 E"
                     /collection_date="2016-07-16"
     gene            49..1053
                     /gene="repM"
                     /locus_tag="OCUAc18_RS19670"
                     /old_locus_tag="OCUAc18_38560"
     CDS             49..1053
                     /gene="repM"
                     /locus_tag="OCUAc18_RS19670"
                     /old_locus_tag="OCUAc18_38560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005404019.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_216071035.1"
                     /translation="MLVVKDNALINASYNLELVEQRLILLAIIEARETGKGINANDALT
                     IHASSYIQNFNVQKHTAYGALKDACRDLFSRQFSYQELSPKGNVINQTSRWVSQIGYIQ
                     EEGSVQLIFAPAVVPLITQLEQQFTSYDLEQVSTLKSKYATRLYEILISWRSVGKTPMI
                     GLEELRGKLGLGVNDYKLLANFKLRVLELALEQINEHTDIFVKYEQHKKGRSIIGFSFK
                     FKQKQQSKIEKPIDQKRDPKTVDMFTGKTDAETKNIARAVVKDTSPPANPLLHRASRIT
                     TSIITHKLHERFKHGDEGLSSTLKRIQGEIMEGGEETADYWQSKLEDFGITFD"
                     /name="repM"
     gene            1117..1305
                     /locus_tag="OCUAc18_RS19675"
                     /old_locus_tag="OCUAc18_38570"
     CDS             1117..1305
                     /locus_tag="OCUAc18_RS19675"
                     /old_locus_tag="OCUAc18_38570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004676352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005404020.1"
                     /translation="MNTRFDPIVSEFESSDHEAEYNAWFIAKVEKSLADSRPTIPHDQV
                     VARMKKRREEREANANR"
                     /name="hypothetical protein"
     gene            1292..1564
                     /locus_tag="OCUAc18_RS19680"
                     /old_locus_tag="OCUAc18_38580"
     CDS             1292..1564
                     /locus_tag="OCUAc18_RS19680"
                     /old_locus_tag="OCUAc18_38580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004676351.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004676351.1"
                     /translation="MLIVKWQPTAVEDLEAIIDYIANHNTVAAYDLEDHILQSAEDLAE
                     HPYKGRNGRRAGTRELLVHPNYWLVYEVSDLIRITNVIHTSRLYP"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1555..1857)
                     /locus_tag="OCUAc18_RS19685"
                     /old_locus_tag="OCUAc18_38590"
     CDS             complement(1555..1857)
                     /locus_tag="OCUAc18_RS19685"
                     /old_locus_tag="OCUAc18_38590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005320422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WP_221255531.1"
                     /translation="MTVEKEPKQKLTLEQKIEQQEQKLKQLKAQKQAAEAREKARKKEQ
                     DRKDDTRIKILLGSYLKKKMDSNPDFNSQILDELENYLTAERDRKLFGLSPLDQG"
                     /name="mobilization protein"
     gene            2082..3662
                     /gene="mobQ"
                     /locus_tag="OCUAc18_RS19690"
                     /old_locus_tag="OCUAc18_38600"
     CDS             2082..3662
                     /gene="mobQ"
                     /locus_tag="OCUAc18_RS19690"
                     /old_locus_tag="OCUAc18_38600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_221255532.1"
                     /translation="MAIYHFSVKAISRSDGRSAVACAAYRAAEKLVCDYYGKEQDYTKK
                     TGVEFTNIYAPENTKKELLDRQKLWNAVEKVENRKNSLLAREFEIAFPSELNAEQRKTL
                     LDELCQELVKKHGVIVDAAIHAPHIEGGSDDRNYHAHIMFTTRSISKTGDFEAKKFRDF
                     SRDDGTKTVSDWREHFADLVNTQLEQIGSNERVSHLSYADLGNGLEATVHEGYAVTQLR
                     RLGIDTEISLANDEIKQRNAEKIENEQVITGLDQEIIVTDAILSDLQRSKAKSDAEHAQ
                     QQQIALERKRERLEAQSLADAHKRATDFHTAFDLNHPDKSLVTQYITDYALLARHGKQP
                     QPPAPVEPPQPTWWQRMTGQQLPPVEQPPFFEIDAIKAIAQSIVEHEQKQAQQQKWQRL
                     QEFLQHSPRTVDRLERDAISRSFDKGQQAGDEELAEFIKYHRQHWQEKEQAAKDWQAEH
                     DRFMREEAAKAREEAALTLQRERESEAKLEAYRERKALEDKEIAEMQQKPVNRDEIQQQ
                     PRPKNDFEP"
                     /name="mobQ"
ORIGIN
        1 atgacctcaa tccagaaagc aaatgcacca ccacgaggaa cagccgatat gttggtagtc
       61 aaagataatg cgttaattaa cgccagttat aatttagagt tagtcgaaca aagattgatt
      121 ttattagcaa ttattgaagc aagagaaaca ggaaagggaa ttaatgccaa tgatgcctta
      181 accatccatg cctcaagtta tattcaaaat tttaatgttc aaaagcatac agcctatggt
      241 gcgttgaaag atgcttgccg agacctattt tcaaggcaat ttagttatca agagttatca
      301 ccgaaaggta atgttataaa tcagacatca aggtgggtga gccaaattgg ttatattcag
      361 gaggaaggta gtgtacaact aatctttgct cctgccgttg ttccacttat tacacagtta
      421 gaacagcagt ttaccagtta cgacctagag caagtctcaa ctcttaaaag caagtatgca
      481 actcgcttgt atgaaatact tatttcatgg cgtagcgtag gcaaaacacc aatgataggg
      541 ctagaggaac tgagaggaaa actaggacta ggtgttaatg actataagct tttagcaaac
      601 tttaaattac gggtattaga gcttgcactt gaacagatta atgaacatac agacattttt
      661 gtaaaatacg agcagcataa aaaaggacgt agcattattg gcttttcatt caagtttaaa
      721 caaaaacaac agtcaaaaat tgaaaaaccg attgatcaga agcgagatcc aaagaccgtt
      781 gatatgttca caggtaaaac tgatgctgag acaaaaaaca tagctagagc agttgttaaa
      841 gacacctcac cgcctgcgaa tcccttgttg catagggcaa gtcgtattac aacgtcaatt
      901 atcacccata aactgcacga acgtttcaaa catggcgatg agggtttatc tagtacattg
      961 aagcgtattc aaggcgaaat tatggaggga ggggaggaaa cggcagacta ctggcaatcg
     1021 aaacttgagg attttgggat aacttttgat taaaaaatac ccatgatttt tgtaccgtaa
     1081 agcagtaaac ttgagcgaat aattggagta ccaactatga atacaaggtt tgatcctatc
     1141 gtgtcagagt ttgaaagttc tgaccatgaa gcagaataca atgcgtggtt tattgcaaaa
     1201 gttgagaaat cactagccga ttcacgcccc accattcccc atgaccaagt agtggcacgt
     1261 atgaaaaaac gtcgtgagga aagagaagcc aatgctaatc gttaagtggc aaccaaccgc
     1321 agtagaagat ttggaagcaa taattgacta tatcgccaac cataatactg ttgccgcata
     1381 tgacctagaa gaccatattc tacaatctgc tgaagatttg gcagaacacc catacaaagg
     1441 gcgtaatggt cgaagagcag gaacaagaga gttacttgtc catccaaatt attggttagt
     1501 ctatgaggtt tcagacctga ttcgcattac caatgtaata catacgagta gactctatcc
     1561 ctgatcaaga ggggatagtc caaacagttt tctatctcgt tcggctgtca gatagttttc
     1621 cagttcatct aaaatttgag aattgaaatc tgggttactg tccatttttt tcttcagata
     1681 agaaccgagt aaaattttta tacgtgtgtc gtcttttctg tcctgctctt ttttacgtgc
     1741 cttttctctt gcttcagcag cttgtttttg tgcctttagc tgctttagtt tttgctcttg
     1801 ctgttcgatt ttctgttcta aagtcagctt ttgctttggc tctttttcga ctgtcatgct
     1861 cttttccaat gccggggatt cttctcgatc ataagcgcat ttgtactggc gtacaagatg
     1921 ctatatgata ctcaaagaat ccccgaaggg cgcacttagc caaactaaat ccatactttc
     1981 agtatgtgat tagtttggtg cgaaagggga ctccccttta cccctgacca acctgttcag
     2041 gttggtaatt tcgataaatc gaaataaaga gcaaaagcaa aatggcgata tatcattttt
     2101 cagttaaagc gattagtcgt agtgatggac gtagtgccgt tgcttgtgct gcatatcgcg
     2161 cggctgaaaa attggtatgc gattactacg gaaaagagca agactacaca aaaaaaacag
     2221 gtgtcgaatt tacaaatatc tatgcacctg aaaacacaaa aaaagaatta ttagaccgtc
     2281 aaaaattgtg gaacgcagtc gagaaagtcg agaacagaaa aaattcactt cttgcccgtg
     2341 aatttgaaat tgccttccca tccgaactaa acgcagaaca gagaaaaacc ctacttgatg
     2401 aactgtgcca ggaacttgtc aaaaagcatg gcgtgatcgt tgatgcagcg atacatgctc
     2461 cgcatattga aggtggatct gatgaccgca actatcatgc tcacatcatg tttacgaccc
     2521 gatctattag taaaactggt gactttgaag ccaaaaaatt cagggacttc agcagagatg
     2581 atgggacaaa aaccgtaagc gactggagag aacattttgc cgatctagtg aatacacagt
     2641 tagagcaaat cggcagcaac gaacgagtaa gccatctcag ctatgcagac ctaggcaacg
     2701 ggctagaagc caccgtacat gagggctacg cagtcacaca gctacgcaga cttggcattg
     2761 atacagaaat cagccttgct aatgatgaaa ttaaacaacg taacgctgaa aaaatcgaaa
     2821 acgaacaggt gattactgga ctagatcaag aaatcatcgt cacagatgcg attttaagcg
     2881 atttacagcg atctaaagcc aaaagtgatg cagaacatgc tcaacaacaa caaatcgctt
     2941 tagaacgcaa aagagagcgt ttagaggcac aatctctagc tgatgcacat aaacgagcga
     3001 cagattttca tacagcgttc gacctcaatc accctgacaa gtctttagta acccagtaca
     3061 tcactgatta cgccttgctt gcccgacatg gcaagcaacc gcaaccccct gccccagtag
     3121 aaccaccgca gccaacatgg tggcaacgga tgacagggca gcagttacca ccagtagaac
     3181 aaccgccatt ttttgaaatt gatgcgatca aagctatagc ccagtccatt gttgagcatg
     3241 agcaaaaaca ggctcaacag cagaaatggc aacggttaca agagttttta caacactccc
     3301 ctcgcaccgt agaccgactt gagcgagatg ctatcagcag atcgtttgat aagggacaac
     3361 aggcaggcga tgaagaactt gccgagttca tcaaatacca tcgtcagcac tggcaggaaa
     3421 aagagcaggc agctaaagac tggcaagccg agcatgaccg ctttatgcgt gaggaagcag
     3481 ctaaagcaag ggaagaagct gcactcacac tacaaagaga gcgtgagtca gaagccaagc
     3541 ttgaagcgta cagagagcgc aaagcattgg aggacaagga aatagctgaa atgcagcaaa
     3601 agcctgttaa tagggatgaa atacaacaac agccacgccc taaaaatgac tttgagccat
     3661 aacaaatttt aatttttcta aacaatacac ggtcttatag agcgagtgtc tacgagcgaa
     3721 caactgaaat ttgcgccttg accctctgaa aaaccgcttt ttacggtttt cgcccctaga
     3781 aagtaactat tttgtgtgct gattttggcg tatctcatct tattaacagt ccataaccct
     3841 tgattttatt ggatttcgtg cgctttttct cttaaaaaag catcgagcga agcgagattc
     3901 atagtcgctt ttgcttttcc caacgcccta gatggtattt gctaaaaact gcccctcaga
     3961 ccgagtgaaa cgaggtcaaa atgccaagag cgaagcgagt gccatagggc agtttttgta
     4021 gtgcttttga gcttttaatg cttttaagag cttttaaaag cttttagacg agctgaaagc
     4081 cttatctagt aatgatttca gagattatat atatacgttt accgacccat atatatacgt
     4141 ttaccgaccc atatatatac gtttaccgac ccatatatat acgtttaccg accatatata
     4201 tacaaataaa ttaatgttat atatttacat aaatttattt gtatgactat ataacataca
     4261 aggcaagtat atgtctcttg gagtaccaac aaa
//
