LOCUS       NZ_AP024808             5049 bp    DNA     circular CON 21-APR-2024
DEFINITION  Acinetobacter baumannii strain OCU Ac18 plasmid pOCUAc18-6, complete
            sequence.
ACCESSION   NZ_AP024808
VERSION     NZ_AP024808.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00330123
            Sequence Read Archive: DRR301936, DRR301937
            Assembly: GCF_019703285.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1
  AUTHORS   Sakiyama,A., Matsumoto,Y., Tsubouchi,T., Suzuki,M., Niki,M.,
            Niki,M., Oinuma,K.I., Kakeya,H. and Kaneko,Y.
  TITLE     Complete Genome Sequence of a Clinical Isolate of Acinetobacter
            baumannii Harboring 11 Plasmids
  JOURNAL   Microbiol Resour Announc 10 (39), e0069521 (2021)
   PUBMED   34591663
  REMARK    DOI:10.1128/MRA.00695-21
REFERENCE   2  (bases 1 to 5049)
  AUTHORS   Tsubouchi,T., Sakiyama,A., Matsumoto,Y., Suzuki,M. and Kaneko,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUL-2021) Contact:Taishi Tsubouchi Osaka City
            University, Bacteriology; Abeno, Asahimachi 1-4-3, Osaka, Osaka
            545-0051, Japan URL :http://www.med.osaka-cu.ac.jp/bacteriology
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Illumina Novaseq; ONT Flongle
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019703285.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 04:56:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,865
            Genes (coding)                    :: 3,655
            CDSs (with protein)               :: 3,655
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 210
            CDSs (without protein)            :: 210
            Pseudo Genes (ambiguous residues) :: 0 of 210
            Pseudo Genes (frameshifted)       :: 58 of 210
            Pseudo Genes (incomplete)         :: 134 of 210
            Pseudo Genes (internal stop)      :: 38 of 210
            Pseudo Genes (multiple problems)  :: 17 of 210
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP024808.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5049
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="OCU Ac18"
                     /isolation_source="venous blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="pOCUAc18-6"
                     /geo_loc_name="Japan"
                     /lat_lon="34.68639 N 135.52 E"
                     /collection_date="2016-07-16"
     gene            1..942
                     /gene="repM"
                     /locus_tag="OCUAc18_RS19645"
                     /old_locus_tag="OCUAc18_38500"
     CDS             1..942
                     /gene="repM"
                     /locus_tag="OCUAc18_RS19645"
                     /old_locus_tag="OCUAc18_38500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871874.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_221255528.1"
                     /translation="MKNELVVKDNALINASYNLEVTEQRLILLAILNARETGNGITADS
                     KLEIHAKDYASRFDISNDGAYKALKNAVLNLFDRQFSFKEQDKKGNVGTVKSRWVSRIK
                     YIDDSATLEITFAPDVVPLITRLEQHFTSYQLKQVSQLTSKYAIRLYELLIAWREVGKT
                     PKIELEDFRSKIGVEDTEYTAMNDFKKRVLEPAIKQINKYTDITATYEQYKKGRVISAF
                     SFSFKQKHQPIIAKTVDLRRDPNTPDFFVKLTDAQRHLFANKLSEMPEMSKYSQGTESY
                     QQFSIRIAEMLLVPEKFRELYPCLEKAGFQPV"
                     /name="repM"
     gene            complement(<1298..1567)
                     /locus_tag="OCUAc18_RS19880"
                     /pseudo=""
     CDS             complement(<1298..1567)
                     /locus_tag="OCUAc18_RS19880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004647572.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            1780..2733
                     /gene="mobQ"
                     /locus_tag="OCUAc18_RS19655"
                     /old_locus_tag="OCUAc18_38520"
     CDS             1780..2733
                     /gene="mobQ"
                     /locus_tag="OCUAc18_RS19655"
                     /old_locus_tag="OCUAc18_38520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017284732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_221255530.1"
                     /translation="MAIYHCTTKTVNRSSGRTAVASMAYRAGEKLTDERTGLTHDFTRK
                     EGVAYTEIISNLDTQIDRAELWNLAEKTENRKDARTAREWVIALPDELDEEQRKELAKD
                     FAKSLVDRYGVVADLAIHAPSKGGDDKNHHAHILLTTRKAELDAENKLVLTQKAEIELS
                     NTKRKSLGMGTSQEEIKQIRATWANLANYALDKAGYKEKIDHRSYAEQGNGLQATIHEG
                     SKVTQLRRKGIDTEVSRFNDNIKLQNSQQLQHKEHQKEKILEQGFNRVEKGFEQWKKDQ
                     EAKRLQLEHQQQLKQQQERAMKQKQHQSMNRSGPSL"
                     /name="mobQ"
     gene            2730..3200
                     /locus_tag="OCUAc18_RS19660"
                     /old_locus_tag="OCUAc18_38530"
     CDS             2730..3200
                     /locus_tag="OCUAc18_RS19660"
                     /old_locus_tag="OCUAc18_38530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_221255526.1"
                     /translation="MSNQNDLDDQLYILLASMKEYREAIADDKRRLETFYSQVASGVLE
                     KAEKSLQDTNKQAIGALKSRIQELDKATSRLNYQFIAVFASAFVALVMVLFLALFLFVP
                     SMDEIQQRRSEVNNLKNYSLDLSKCDGKTCVRIIKKQCGYGKNGDYCVIDPK"
                     /name="hypothetical protein"
     gene            3630..4139
                     /locus_tag="OCUAc18_RS19665"
                     /old_locus_tag="OCUAc18_38540"
     CDS             3630..4139
                     /locus_tag="OCUAc18_RS19665"
                     /old_locus_tag="OCUAc18_38540"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_221255527.1"
                     /translation="MENKDVAVKSKPSKPAAKRLALRTRIFGSLEDVELWHRGTDDGYI
                     HLPRVLPLVVNIIDALTKGKPAGQTYLALWCRNYDEMIVDVTDEASLAYESGFYSERRV
                     TTWQQRMQSLHDLGFVKAHKVGSRYQYVLMLHPIHAIKALYEEKKIQQEMYEIFEHRAY
                     EVGALK"
                     /name="hypothetical protein"
     Typing          1781..2730
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.114"
                     /coverage="100.0"
                     /evalue="9.68e-174"
ORIGIN
        1 atgaaaaatg agcttgttgt gaaggataat gcgttaatta atgctagtta taatttagaa
       61 gtaactgaac agcgtttaat acttttagcg attcttaatg caagagaaac aggcaacggt
      121 attacagccg atagcaaatt agaaatacat gcaaaggact atgccagccg ctttgacata
      181 agcaatgatg gagcttataa agcattaaaa aatgctgtct taaacttatt tgatcgccag
      241 ttttctttca aagaacaaga taagaaaggg aatgtaggta cagtaaaatc aagatgggta
      301 agccgtatta aatatattga tgattcagcc acattagaga ttacgtttgc acctgatgtt
      361 gtcccattaa ttacaagact tgagcagcat ttcacgagct atcaacttaa acaagtcagc
      421 caacttacca gtaaatacgc aatccgttta tatgagcttt taattgcatg gcgtgaagtt
      481 ggaaaaacac caaaaattga attggaggat tttagaagta aaattggtgt tgaagatact
      541 gaatacacag caatgaatga ttttaaaaag cgtgtattag agcctgctat taagcaaatt
      601 aataaatata cagacattac tgcaacgtat gagcaatata aaaaggggcg ggtaatttca
      661 gcattttcat tcagctttaa gcagaagcac caaccaataa ttgcaaagac agtggacctt
      721 agaagagatc caaacacccc tgactttttt gtcaaattga ctgatgctca acgtcattta
      781 ttcgcaaata aactttccga aatgccagaa atgagtaaat attctcaagg tactgaaagc
      841 taccaacaat tttctattcg tattgctgag atgcttttag tacctgaaaa atttagagag
      901 ctttatcctt gcttagagaa agctgggttt cagccagttt aaaacactag gttcaatagc
      961 ggttagtaag gctagtatcg taacctgctg gatacccgtt aagccctaca ttcgtaacct
     1021 tgtgatcact atcaaaatct ggctcccaat cttcatcttc atcttctgtg tgaagttcct
     1081 catatgatgg attgtagaaa atatctgcca gttcaatata gtgatcgcca aactcttcga
     1141 ctccaacact tagcacccac aaaccattcg attccttaat aaaattaaag acattaaagt
     1201 gagcaacaaa tttttggtag aaactattgg cttctgcaga tgacttaaat tctttatcaa
     1261 aaaaaacgag aggtttttct tgagcttcat tcataagagg taaatcaaaa agctgtctat
     1321 ctctattttc tgttaaatat tcattaagtt cagcgagtat tttttcttta ttggcttcat
     1381 tactttgcat ctttttaatt aagtaagaac caagcaaaat tttacgtcga gtatcgtcct
     1441 ttctttcctg ctctttttgc ttcgattttt ctctagcttc gatagcctgt ttacgagcct
     1501 ttaactgttt cagcttttct aattgggctt cgattttctt ttcgagattt tcagcagctt
     1561 ttgtcatgat tcagatttcg ggatatagaa aaccactcaa gcataaggcc ctatgaattg
     1621 gaattcaagc gggctatatg ctatcgtgtt tttcatgaaa tgcgcactta cgacttgaac
     1681 aaggttcaag tcaaatttgc gtaaggggaa accccttaac cccaaaaaat tgctacgcaa
     1741 tttttaaaag caaaagcaaa accatcacaa ggctaaggca tggcaattta tcactgtacg
     1801 acgaagactg ttaaccgaag ttcaggacga actgctgttg catctatggc ctatcgtgca
     1861 ggggaaaaat tgacagatga acggacagga ttaacgcacg actttacaag aaaagaaggt
     1921 gtggcttata ccgaaattat ctcgaactta gatactcaaa ttgatcgtgc agaactctgg
     1981 aatctcgctg aaaaaaccga aaaccgaaaa gatgccagaa ctgctagaga atgggttatt
     2041 gctttgcctg atgaattaga tgaagaacaa cgcaaggaac tcgctaaaga ctttgccaag
     2101 tccttagtag atcgctatgg ggtagttgca gatttagcta tccatgcacc cagcaaaggt
     2161 ggtgatgata aaaaccatca tgcacatatc ttgctgacaa cgcgcaaagc agaattagac
     2221 gctgaaaata aattggtcct cactcaaaaa gcagaaattg agctaagtaa caccaaaaga
     2281 aaaagcttag gcatgggaac aagccaggaa gaaatcaaac agattcgagc cacctgggca
     2341 aaccttgcaa attacgccct ggacaaagca gggtacaaag aaaaaataga tcatcgcagc
     2401 tatgccgaac aaggtaacgg cctacaagcc acgatacatg aaggcagcaa ggttactcag
     2461 ttacgcagaa agggcataga tactgaagtt agtcgtttca atgacaatat caaacttcaa
     2521 aacagccagc agctccaaca caaagaacac caaaaggaaa aaatcctaga acaaggcttt
     2581 aaccgtgttg aaaaggggtt tgagcaatgg aagaaagatc aggaagctaa acgcctacaa
     2641 ctggaacatc agcagcagtt aaagcaacag caagaacgag ccatgaagca gaagcaacac
     2701 caaagcatga atagaagtgg gccatcgcta tgagcaacca aaatgattta gacgaccagt
     2761 tatatatttt attggcttcc atgaaggaat acagagaagc catagcagac gataagaggc
     2821 gattagagac attttacagc caagtagcta gtggggttct agaaaaggct gaaaagtcgc
     2881 tacaagacac aaataaacag gctatagggg cattaaagag ccgtattcag gagttagaca
     2941 aggcaacaag ccggcttaac tatcaattca ttgctgtttt tgccagtgct tttgtagcgt
     3001 tggtaatggt gttgtttttg gcgttatttt tatttgtacc aagtatggat gaaatccaac
     3061 aacgcagatc cgaagtaaac aatctcaaaa actacagttt agacctatca aaatgtgacg
     3121 gtaaaacatg cgttagaatt ataaaaaaac agtgtggcta tgggaaaaat ggcgattatt
     3181 gtgtgattga ccctaaataa atgctgcttc ttactttgct ctgttccttt taaatctatc
     3241 agatatctag tgtcgcaaag tggctcgact ttgaaacttg gccttagcca tagttcaaag
     3301 tgtcgagctt taaagctatt tttgaccaaa aataaatgta ataatatgta attaatttgc
     3361 taactggacg acgggatcac aggattacgt agattacgga ggacagataa cgaggattac
     3421 tggataacgt aaaaataatt ataaaacaaa tatttaacat cgttttctgt aatatatatt
     3481 acttaaatta aaattatttg aattttctat tacaataaaa caaataatta tgtatattaa
     3541 atttcaaaga aaaatattca tagatcctga ttaacctatt taatccactt ctcatgacat
     3601 attaaatatt tgattttcaa ggaattttta tggaaaataa agatgttgcc gtgaaaagca
     3661 aaccatccaa gccagcggct aaaaggttag cgttaagaac gcgaatcttt gggagtttgg
     3721 aggatgttga gctttggcat cgaggtactg atgatgggta tatccaccta ccgcgggttt
     3781 tgccgttagt agtaaatatt attgatgctt taacaaaagg gaagccagcg ggacaaacgt
     3841 acttagcatt atggtgtcgt aactatgatg agatgattgt agatgtcact gatgaagctt
     3901 ctttagcgta tgaatctggt ttttatagtg aaagacgagt aactacttgg caacaaagaa
     3961 tgcagtcact tcacgatctc gggtttgtta aagcacataa agttggaagt cgctatcagt
     4021 acgtattaat gcttcatcca attcatgcga tcaaagctct ctatgaagag aaaaagatcc
     4081 agcaggaaat gtatgaaatt tttgaacata gagcctatga agtaggagca cttaaatgaa
     4141 attaagtttt gaaaatcaag gccttagggt ggacagagat gtccttaata taacattgcg
     4201 aagggagggt gattatgagc gagctcattc acgcgatctt agtagaaatg ccagtctata
     4261 tgtggatagc tgtctatctt ctggcatgga gaactaagat cacgataaaa attacacaca
     4321 accgataact aatattcttg caggaataaa aagttaatag ataaggaggt gaaaaattga
     4381 agaaggcgag tctgaccact cgtctttttc tatattaaaa cagtagaatt aattatttaa
     4441 cccctgtttt aaataatacg tttcagtcac atctttgtac ttcattctct gtattttcaa
     4501 cttaatcatt ttttattaac cgagcgagtg tctacgagcg acctcttggc atcaaaattc
     4561 atactttttt ctctgattac aattgctttc tattgcttca taaaaggtat aacagagcaa
     4621 agcgactgta cttcaattaa tttatgaccg agcgaagcga gcacaaaatt tctgagcgta
     4681 gcgaaggcat agcttttatt aaactctcaa ctaacatttg aaaaaacagc ccctcaaccg
     4741 agctttagcg aggttaaaac gtccgagctt tagcgagggc atagggctgt tttcatacct
     4801 tcttaggctt ttatatattt taaagctttt aaatgctttt aagaccactg aaagtccttc
     4861 cacaaaagtc tttcagcgct tataaaggga catttagtcg gttataaagg gacatttagt
     4921 cggttataaa gggacattta gtcggttata aagggacatt tagtcggaat aaagggacat
     4981 tgtaagtatt gaccctttag tgacttcaat ttacactgtc cgtattataa gtaacaatat
     5041 aatttgact
//
