LOCUS       NZ_AP024807             7104 bp    DNA     circular CON 21-APR-2024
DEFINITION  Acinetobacter baumannii strain OCU Ac18 plasmid pOCUAc18-5, complete
            sequence.
ACCESSION   NZ_AP024807
VERSION     NZ_AP024807.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00330123
            Sequence Read Archive: DRR301936, DRR301937
            Assembly: GCF_019703285.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1
  AUTHORS   Sakiyama,A., Matsumoto,Y., Tsubouchi,T., Suzuki,M., Niki,M.,
            Niki,M., Oinuma,K.I., Kakeya,H. and Kaneko,Y.
  TITLE     Complete Genome Sequence of a Clinical Isolate of Acinetobacter
            baumannii Harboring 11 Plasmids
  JOURNAL   Microbiol Resour Announc 10 (39), e0069521 (2021)
   PUBMED   34591663
  REMARK    DOI:10.1128/MRA.00695-21
REFERENCE   2  (bases 1 to 7104)
  AUTHORS   Tsubouchi,T., Sakiyama,A., Matsumoto,Y., Suzuki,M. and Kaneko,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUL-2021) Contact:Taishi Tsubouchi Osaka City
            University, Bacteriology; Abeno, Asahimachi 1-4-3, Osaka, Osaka
            545-0051, Japan URL :http://www.med.osaka-cu.ac.jp/bacteriology
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Illumina Novaseq; ONT Flongle
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019703285.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 04:56:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,865
            Genes (coding)                    :: 3,655
            CDSs (with protein)               :: 3,655
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 210
            CDSs (without protein)            :: 210
            Pseudo Genes (ambiguous residues) :: 0 of 210
            Pseudo Genes (frameshifted)       :: 58 of 210
            Pseudo Genes (incomplete)         :: 134 of 210
            Pseudo Genes (internal stop)      :: 38 of 210
            Pseudo Genes (multiple problems)  :: 17 of 210
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP024807.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7104
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="OCU Ac18"
                     /isolation_source="venous blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="pOCUAc18-5"
                     /geo_loc_name="Japan"
                     /lat_lon="34.68639 N 135.52 E"
                     /collection_date="2016-07-16"
     gene            1..951
                     /gene="repM"
                     /locus_tag="OCUAc18_RS19590"
                     /old_locus_tag="OCUAc18_38390"
     CDS             1..951
                     /gene="repM"
                     /locus_tag="OCUAc18_RS19590"
                     /old_locus_tag="OCUAc18_38390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786597.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_221255525.1"
                     /translation="MRELVVKDNALINASYNLDLVEQRLILLAIVEARESGKGINANDP
                     LEVHAEGYINQFGVHRNTAYQALKDACNDLFARQFSYQKINERGNIENYRSRWVSEIGY
                     VDNEAVVKLIFAPAIVPLITRLEEHFTKYELQQVSNLSSAYAVRLYELLIAWRSTGSTP
                     VIELSDFRQRIGVLDTEYKRMERFKTSVLELAIKQINEHTDITVKYEQHKRGRSISGFS
                     FTFKQKKKDNPPIERDPNTLDLFTKMTDAQRHMFANKLSELPEMGRYSQGTESYQQFAV
                     RIAEMLQDPAQFKELYPYLKKVGYMPSNKKDTVNG"
                     /name="repM"
     gene            944..1519
                     /locus_tag="OCUAc18_RS19595"
                     /old_locus_tag="OCUAc18_38400"
     CDS             944..1519
                     /locus_tag="OCUAc18_RS19595"
                     /old_locus_tag="OCUAc18_38400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005102333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_032042705.1"
                     /translation="MAKLSLSEVSKKFHVDRSTIYRAVRNGRLSRSSDGQFDLAEVIRC
                     FGEPEQTSQKIESSKQEGDESTKKLIAHLENEVKKYQEREERLMQQIDRMQTLIELKSV
                     APATAAPHQDATACDTKMPQHATTQQDTDNKNNNELNIAENVAVPQQETTAYHTQTLQH
                     ATLQSVAVPQHKKRGLFGRVLNAVFDND"
                     /name="plasmid replication DNA-binding protein"
     gene            1539..1682
                     /locus_tag="OCUAc18_RS19600"
                     /old_locus_tag="OCUAc18_38410"
     CDS             1539..1682
                     /locus_tag="OCUAc18_RS19600"
                     /old_locus_tag="OCUAc18_38410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001125247.1"
                     /translation="MPKLKDIALGIIVAPLLIPIMLIASYQDKKALKKELDERQKEKDQ
                     AS"
                     /name="hypothetical protein"
     gene            1884..2219
                     /locus_tag="OCUAc18_RS19605"
                     /old_locus_tag="OCUAc18_38420"
     CDS             1884..2219
                     /locus_tag="OCUAc18_RS19605"
                     /old_locus_tag="OCUAc18_38420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032866032.1"
                     /translation="MKIGIFWFLQKQVIGIAHPFNLNDADSIGLIDSPYTHVDYWKNMQ
                     SVYPELRHYEYEQIPRGRVVFDANKEKAIVYMDKKLFNTVIATKIYDFFDIDSENAIPR
                     KDPHYRT"
                     /name="hypothetical protein"
     gene            2269..2448
                     /locus_tag="OCUAc18_RS19610"
                     /old_locus_tag="OCUAc18_38430"
     CDS             2269..2448
                     /locus_tag="OCUAc18_RS19610"
                     /old_locus_tag="OCUAc18_38430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016658770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001231652.1"
                     /translation="MRKRLKDYSLDQISNREKVSGQVLPIQFSNDEATKRVVMHSAKRV
                     INQHKAEIQELAYK"
                     /name="hypothetical protein"
     gene            2445..2831
                     /locus_tag="OCUAc18_RS19615"
                     /old_locus_tag="OCUAc18_38440"
     CDS             2445..2831
                     /locus_tag="OCUAc18_RS19615"
                     /old_locus_tag="OCUAc18_38440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004769969.1"
                     /GO_function="GO:0016301 - kinase activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="WP_000053135.1"
                     /translation="MSLISSTFVIAVHDEILLQTGVGRIGCHIEKLESVLSRIDQQMYY
                     SNVSDIFEIAAWFGIAISKGHAFVDGNKRTGLAVMLTYLEIQGVTIQEQTGLDDLMVDI
                     VESQEEHELLAKIVAEFLYELSII"
                     /name="type II toxin-antitoxin system death-on-curing
                     family toxin"
     gene            complement(2833..3384)
                     /locus_tag="OCUAc18_RS19620"
                     /old_locus_tag="OCUAc18_38450"
     CDS             complement(2833..3384)
                     /locus_tag="OCUAc18_RS19620"
                     /old_locus_tag="OCUAc18_38450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016658769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="WP_005401799.1"
                     /translation="MKKVHLIQADITAFAVHAIVNSANKSLLGGGGLDYVVHKKAGPLM
                     KEECIRLNQQKGGCPTGQAEVTTAGNLPAKYLIHAVGPRWLDGEHNEPQLLCDAYSNAL
                     FKANEIHALTVSFPCISTGVYGFPPQKAAEIAIGTILSMLPQYDHVAEVFFICREDENY
                     LIYKNLLSNIDDPNIQILIS"
                     /name="macro domain-containing protein"
     gene            complement(3393..4091)
                     /locus_tag="OCUAc18_RS19625"
                     /old_locus_tag="OCUAc18_38460"
     CDS             complement(3393..4091)
                     /locus_tag="OCUAc18_RS19625"
                     /old_locus_tag="OCUAc18_38460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005401798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_057692832.1"
                     /translation="MEKSLNLANSIFAGFNDKNGLMICGYEWGWSKEDQKLDESDSRPT
                     VDMSIECTFSNKSLRYGPSANTWPYDKNIKKWFKFWGHPLNNENLGSDFDKCIIQTNWA
                     YTSNADIESYNRFLEKEAVDNFIHHIEVLRPKVILFMGSKLINFLRNIDVWDRFTAIVG
                     PEIEPLKMVQKTDFDGTRFKVYFDNFEQCQVVCLPHPSSSRGLSDEYIKLFEPELGTII
                     TEYKKQKGIL"
                     /name="hypothetical protein"
     gene            complement(4211..4642)
                     /locus_tag="OCUAc18_RS19630"
                     /old_locus_tag="OCUAc18_38470"
     CDS             complement(4211..4642)
                     /locus_tag="OCUAc18_RS19630"
                     /old_locus_tag="OCUAc18_38470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004986359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000390052.1"
                     /translation="MEDPTLNELIRNTELFFEHHWNVDLLGTPPEWSPVHFNFGKIPNY
                     DKQGVYAFIKGDLVTYIGVGTSRGAGRYRGNGLSARVMKYCRWNEDKTEYIAIDPRLKD
                     AGSIITIGFERDRAYLANALEIFLISKLQPVHNVVKVGM"
                     /name="hypothetical protein"
     gene            complement(4660..4926)
                     /locus_tag="OCUAc18_RS19635"
                     /old_locus_tag="OCUAc18_38480"
     CDS             complement(4660..4926)
                     /locus_tag="OCUAc18_RS19635"
                     /old_locus_tag="OCUAc18_38480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_006581723.1"
                     /translation="MSVTETLDSKIKAQEEKLKQLKAQRQAALARERAKEKEQARKDDT
                     RRKILIGSCMLKITEEDEQARAKLIAQMDKYLTDERDRKLFNL"
                     /name="hypothetical protein"
     gene            5136..6572
                     /gene="mobQ"
                     /locus_tag="OCUAc18_RS19640"
                     /old_locus_tag="OCUAc18_38490"
     CDS             5136..6572
                     /gene="mobQ"
                     /locus_tag="OCUAc18_RS19640"
                     /old_locus_tag="OCUAc18_38490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005248576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_057050431.1"
                     /translation="MAIYHFSVKTVARSAGRSATAAIAYRAGEKIYCEREGREHDYSRK
                     TGVEYKEIYLPEGAPQHLKNREKLWNEVEQRETRKNSTVAREFEIAFPSELNQEQRLAM
                     LEELCASIVERHQVAVDACIHAPHTGSGSDERNYHAHILMSTRKLTPEGFTEKTRELDQ
                     KHSGEIEHWREHFADICNMHLDMAGFTARVDHRSYKDQENGLEATLHEGPKVTELRRRG
                     IETEISRINDAIKERNQTQLQYGKNMDLLIAENEITLKKLKTEQQMHIQNSAKTPPLSA
                     EEQFQYIQRENLSKLLKGEISAKEANLDLDFMQHNLEKVEGILSKQQKYKNDFNQQLAQ
                     DIVKSRLKQSHDKLQSLVDQHNELGQNKPLLFGKKAWEAQRDAIYQEHKQLKDRHEYQK
                     KNGVKDLLENEKFKAHAWTQYQKEHPTKAEQYQNLYQSYRAIKNCVDKIKAEQQMKLRQ
                     EQQLKAQQQAPKMKSRGMRR"
                     /name="mobQ"
     Typing          2..951
                     /name="rep_cluster_1172"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.423"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5137..6569
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.117"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgagagaat tagtggtaaa agacaatgcc ttaatcaatg caagctataa cttagattta
       61 gtagaacaac gtttaatttt attggccatt gttgaggcaa gagaaagcgg gaaaggtatt
      121 aatgcgaatg atccccttga agtacatgca gaaggctata tcaatcaatt tggcgtacat
      181 cgcaatacgg cttatcaagc attaaaagat gcctgtaatg atttatttgc aagacaattt
      241 agctatcaaa aaataaatga acgagggaat attgagaact atagatcccg ttgggttagt
      301 gaaattggat atgtagataa tgaagcagtg gttaaactta tctttgcccc agccatagtt
      361 cccttaatta cacgcttaga agagcatttc actaaatacg aattgcagca agttagtaat
      421 ctcagcagtg cttatgctgt tcgcttatat gaattattaa ttgcttggag aagtactggc
      481 tctactcctg ttatagagct aagtgatttc cgtcaaagaa ttggcgtact cgatacagag
      541 tacaagcgta tggaacgctt taaaactagt gtacttgagc ttgctattaa acaaattaac
      601 gaacatacag atatcactgt gaagtatgag caacacaaaa gaggtcgatc aatttcagga
      661 ttctctttta cttttaaaca gaaaaagaag gacaacccac cgatagaaag agatccgaat
      721 acgttagatc tctttacaaa gatgactgat gcacaacgcc atatgtttgc aaataaactt
      781 tctgaacttc ctgaaatggg tcgttattcc cagggaactg aaagctacca acagtttgct
      841 gtacgtattg ctgagatgct acaagatccc gctcaattca aagagctata cccataccta
      901 aaaaaagtgg gatacatgcc atcaaataaa aaggacaccg taaatggcta agttatcact
      961 aagtgaagta tctaaaaaat ttcatgtgga tagatcaacc atttacagag ctgtacgtaa
     1021 tggacgttta tcacgctcca gtgatggaca attcgatcta gcagaggtca tacgatgctt
     1081 tggagaacct gagcaaacat ctcaaaaaat tgaatcatct aagcaagaag gtgatgaatc
     1141 tacaaaaaaa cttattgccc atttagaaaa tgaagtcaaa aaataccaag aacgtgaaga
     1201 acggttaatg caacaaattg accgtatgca aacactcatt gagctgaaaa gtgttgcacc
     1261 tgccacagca gcaccacacc aagatgctac ggcatgcgac accaagatgc cacagcatgc
     1321 gacaacacaa caagacactg ataacaaaaa taataatgaa ttaaatattg cagaaaatgt
     1381 agcagtgcca cagcaagaaa ctacggcata ccacacccaa acgctacagc atgccacgtt
     1441 gcaaagtgtg gcagtgccac aacacaaaaa acgtggttta tttggccgtg tgctgaatgc
     1501 cgtctttgat aatgactgag caataagggt gaaagatcat gccgaaactg aaagacattg
     1561 ccctgggaat tattgtagct ccgctgctga tcccgatcat gctgattgca tcgtaccagg
     1621 ataaaaaggc actcaaaaaa gagctggatg aacgccaaaa agaaaaagac caggcatcct
     1681 gattattcaa ttgagctaat caaaagtgtg gaacattgat gaaataatag gcgtggaacg
     1741 tgaaagatca caaaaaaaag cccgtacagg gatgttcggg ctataaaata gaaactacag
     1801 cattgatttc tatagataat tataacgcat ttcgtataag gtgtattatg ttaattttag
     1861 ggaaattaat cagttgatga aaaatgaaaa taggaatttt ttggtttttg caaaagcaag
     1921 ttatcggcat agctcatcca ttcaatttaa atgatgcaga ttcaataggc ttaattgata
     1981 gtccatatac acatgtcgat tattggaaaa atatgcaaag tgtataccca gaactacgtc
     2041 attatgaata tgaacagatt ccaaggggta gggtagtttt cgacgcaaat aaagaaaaag
     2101 caatcgtgta tatggacaaa aaattattta acacagtaat agccactaaa atttatgatt
     2161 tctttgatat agattcagaa aatgctattc caagaaaaga tcctcactat cgtacttaat
     2221 tgtttaattt aggctaaaat gaaattctct tttaataggg gtatgaagat gagaaagcgt
     2281 ttaaaagact atagccttga tcaaatttca aatcgtgaaa aggtttcggg tcaagtatta
     2341 ccaatccaat ttagcaatga cgaagcgact aaacgtgttg tgatgcatag tgcaaaacga
     2401 gtcattaatc agcataaagc tgaaatccaa gagcttgctt ataaatgagt ttgattagct
     2461 caacattcgt cattgctgtg catgatgaga tcctattaca aactggcgta gggcgaatag
     2521 gctgtcatat tgaaaagcta gaaagcgtat taagccgtat agatcagcag atgtattaca
     2581 gtaatgtaag cgacattttt gagatcgcag catggtttgg tatagctatc tcaaaaggtc
     2641 atgcttttgt tgatggtaat aaaagaactg gtttagctgt aatgctgact tatctcgaaa
     2701 ttcaaggtgt taccattcag gaacaaacag gtttagatga tttgatggtc gatattgttg
     2761 aatcacaaga agaacatgaa ttattagcca aaattgtggc tgaattctta tacgagcttt
     2821 cgataattta aactaagaaa tcagaatctg aatatttgga tcatcgatat tcgataaaag
     2881 attcttataa attaaataat tttcatcctc tcggcagata aaaaatactt ctgcaacatg
     2941 atcatattga ggcaacatag agagtatagt accaatcgca atttcagcag ccttttgagg
     3001 tggaaaacca taaacacctg tactaataca tggaaaagaa acagtgagtg catgaatttc
     3061 attagctttg aatagtgcat tactataagc atcacaaagc aattgtggct cattgtgttc
     3121 gccatctaac catcgtggtc caaccgcatg aataaggtat ttagcaggca gattacctgc
     3181 tgttgttact tctgcttgac ctgtagggca tccacctttt tgttgattta gacgaataca
     3241 ttcttctttc atcaatggtc cagctttttt atgaaccaca taatcaagtc ccccaccacc
     3301 aagcaaagat ttatttgctg agttaacgat ggcatgtaca gcaaaagctg ttatatctgc
     3361 ttggatgaga tggacttttt tcataattag ttttatagaa tacccttttg tttcttatat
     3421 tcagtaatga ttgttcctaa ttctggttca aataatttga tatattcatc agatagacct
     3481 ctactactag atggatgtgg taagcacaca acttggcatt gttcaaaatt atcaaagtaa
     3541 actttaaatc gagtaccatc aaaatctgtt ttttgtacca tttttaaggg ttctatttct
     3601 ggaccaacaa tagcagtaaa cctatcccaa acatctatat ttctcagaaa attaataagc
     3661 ttactaccca taaataaaat tactttgggt ctcaaaactt caatatgatg aataaaatta
     3721 tcaactgctt ctttttctaa gaaacgatta tagctttcta tatctgcatt actcgtatag
     3781 gcccaattag tttgaataat acatttatca aagtctgaac ctaaattttc attgtttaat
     3841 gggtgtcccc aaaacttaaa ccatttttta atatttttat cataaggcca agtattggca
     3901 gaaggaccat accttaggga tttattacta aatgtacatt caatgctcat atctactgtg
     3961 ggacggctat cgctttcatc aagtttttga tcttcttttg accatcccca ttcatagcca
     4021 caaatcatta atccgttttt atcattaaag cctgcgaaaa ttgaatttgc taaatttaga
     4081 cttttttcca ttttatcttc tctgatactt ttaagattca aattatcaca tatatttcat
     4141 ataaatcatt tatatggaaa gttttttata gatataaaaa tacacttaac ataaaatctc
     4201 ttatgcgaaa ttacatacca actttaacca cattgtggac aggttggagt ttagagataa
     4261 gaaaaatttc tagagcattt gctaaataag ccctatcacg ttcaaagcct atcgtaatga
     4321 tggatccagc atcttttaat cttggatcta tcgcaatata ttcagtttta tcttcattcc
     4381 atcggcaata tttcattacc ctggcgctta agccattacc acgataacgg ccagcacctc
     4441 tagaagtgcc aacaccgata taagtcacta aatccccttt gataaacgca tatacacctt
     4501 gtttatcgta attaggaatt ttcccaaaat taaaatgtac tggtgaccat tcaggaggcg
     4561 ttcctaatag atctacattc cagtgatgtt caaaaaataa ttctgtatta cgtattaact
     4621 cattaagtgt tggatcttcc actactcatc tctcaaaatt tataaattaa aaagctttcg
     4681 gtcacgctca tctgtcaaat acttatccat ctgagcaatt agtttagctc tggcttgttc
     4741 atcttcttca gtgattttta gcatgcagga tccaattaaa attttgcgtc tggtatcgtc
     4801 ctttcgtgcc tgttctttct ctttggcacg ttctcttgcc agtgctgcct gtctttgagc
     4861 ctttaactgc tttagctttt cttcctgtgc cttgattttg ctgtctagtg tttcagtaac
     4921 gctcattaat ccaataccat catcctggga aaccatccct aacataagcg cacttgaata
     4981 tgcattcaag gtgctatatg tatagtaatg ttttcctcga agagcgcact tatgcaaact
     5041 tcgtttacaa gtacgattgg ggagacccca ataccccgac aaccgtattc acggttgtat
     5101 ttcactacgt gaaaatttaa aagcgaaaaa caggcatggc gatttaccat ttttcagtca
     5161 aaaccgtagc acgatcagca gggcgttccg ccactgctgc gattgcctat cgggcaggtg
     5221 aaaagatcta ttgtgaacgt gaaggccgag aacatgacta cagccgaaaa actggggtgg
     5281 aatacaaaga gatttactta cccgaaggcg caccacagca tttaaaaaat cgggaaaaac
     5341 tttggaacga agtcgaacaa cgagaaaccc gaaaaaactc aacagtggcc cgggaatttg
     5401 aaattgcctt tccaagtgaa ctgaaccagg aacaacgtct ggccatgctc gaagaattat
     5461 gtgccagcat cgtggaaaga caccaagtag ccgttgatgc ctgcatacat gcaccgcata
     5521 ccggatccgg cagtgatgag cgcaattatc acgcccatat cctgatgagc acacgtaaac
     5581 tcacgccaga gggttttaca gaaaaaaccc gagagctgga tcagaaacac agtggagaaa
     5641 tcgagcactg gcgcgaacac tttgccgata tttgcaatat gcacctcgac atggccggat
     5701 tcaccgcccg ggtcgaccat cgtagctaca aagaccagga gaatggctta gaagccaccc
     5761 tgcacgaagg accgaaagtc accgaactac gcagaagagg catcgaaact gaaatcagcc
     5821 ggatcaatga tgcaatcaaa gaaagaaatc agactcagct gcaatacggc aaaaatatgg
     5881 atctattgat tgctgaaaat gagatcacac tcaaaaaact aaaaacagaa cagcagatgc
     5941 atatccaaaa tagcgctaaa acgccaccat taagcgcaga agagcaattt caatacatac
     6001 agagagaaaa cctaagcaaa ctactcaaag gcgaaatcag cgcaaaagag gcgaatttag
     6061 acctagattt tatgcaacac aatcttgaga aagtcgaagg tatcctgagc aagcaacaaa
     6121 agtacaaaaa tgacttcaat cagcagctcg cccaagacat tgtgaaaagc agactcaaac
     6181 aaagtcatga caaattgcaa agcctggtgg atcaacacaa cgagttaggt cagaacaagc
     6241 ccttgctgtt tggaaaaaaa gcttgggagg cacaacgtga tgcaatttac caggaacaca
     6301 aacaactgaa agatagacat gaataccaga aaaaaaatgg tgtgaaggat ttattggaaa
     6361 acgaaaaatt caaagcacat gcctggacac agtaccaaaa agagcatccg accaaagccg
     6421 agcaatatca gaacctctac caatcttacc gagctattaa aaactgtgtg gataagatca
     6481 aagcagaaca acaaatgaaa cttagacaag aacaacagct caaagcacag caacaagctc
     6541 ctaaaatgaa atctcgtggc atgaggcgat aaatagagtg agtgtctacg agcgaactga
     6601 aaaaatttgc ccatcccttt ctttgattac aagctttcta tccatcaatc acctgcttta
     6661 cagagcatcc cgaatgggtg cgaactttcc aagtttgctt cctaaatcga gcgaagcgag
     6721 aaaaaaagct catgagcgaa gcgaattccg agttgctttt gctttttcta aaattcacga
     6781 aaatatgaac caaaaattgc cccgacgaat cgagcgaaag cgagattcaa tagagtttga
     6841 gcgaacgaag tgaagcgaaa accaagggca atttttcact ccttaggctt ttaattattt
     6901 taaagttttt aaatgctttt agatatgctg aaagcctttg ttcacaatgc ttttaaggtt
     6961 tatatgacac cgtttacctt gcaatatgac accgtttacc ttgcaatatg acaccgttta
     7021 ccttgcaata tgacaccgtt taccttgctt atagcactaa aattaattat tgtgtcatag
     7081 cataataatt aatactggtg cttc
//
