LOCUS       NZ_AP024806             8948 bp    DNA     circular CON 21-APR-2024
DEFINITION  Acinetobacter baumannii strain OCU Ac18 plasmid pOCUAc18-4, complete
            sequence.
ACCESSION   NZ_AP024806
VERSION     NZ_AP024806.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00330123
            Sequence Read Archive: DRR301936, DRR301937
            Assembly: GCF_019703285.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1
  AUTHORS   Sakiyama,A., Matsumoto,Y., Tsubouchi,T., Suzuki,M., Niki,M.,
            Niki,M., Oinuma,K.I., Kakeya,H. and Kaneko,Y.
  TITLE     Complete Genome Sequence of a Clinical Isolate of Acinetobacter
            baumannii Harboring 11 Plasmids
  JOURNAL   Microbiol Resour Announc 10 (39), e0069521 (2021)
   PUBMED   34591663
  REMARK    DOI:10.1128/MRA.00695-21
REFERENCE   2  (bases 1 to 8948)
  AUTHORS   Tsubouchi,T., Sakiyama,A., Matsumoto,Y., Suzuki,M. and Kaneko,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUL-2021) Contact:Taishi Tsubouchi Osaka City
            University, Bacteriology; Abeno, Asahimachi 1-4-3, Osaka, Osaka
            545-0051, Japan URL :http://www.med.osaka-cu.ac.jp/bacteriology
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Illumina Novaseq; ONT Flongle
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019703285.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 04:56:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,865
            Genes (coding)                    :: 3,655
            CDSs (with protein)               :: 3,655
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 210
            CDSs (without protein)            :: 210
            Pseudo Genes (ambiguous residues) :: 0 of 210
            Pseudo Genes (frameshifted)       :: 58 of 210
            Pseudo Genes (incomplete)         :: 134 of 210
            Pseudo Genes (internal stop)      :: 38 of 210
            Pseudo Genes (multiple problems)  :: 17 of 210
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP024806.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8948
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="OCU Ac18"
                     /isolation_source="venous blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="pOCUAc18-4"
                     /geo_loc_name="Japan"
                     /lat_lon="34.68639 N 135.52 E"
                     /collection_date="2016-07-16"
     gene            1..936
                     /gene="repM"
                     /locus_tag="OCUAc18_RS19545"
                     /old_locus_tag="OCUAc18_38310"
     CDS             1..936
                     /gene="repM"
                     /locus_tag="OCUAc18_RS19545"
                     /old_locus_tag="OCUAc18_38310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237873.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_005237873.1"
                     /translation="MPTSELIVKDNALINASYNLDTVEQRLILLAILQARETKTGIDAN
                     TRLRIHASDYMSRFNVSKHAAYKALKTAVTNLFGRQFSYQTIDEKTGKSKKVISRWVQN
                     ISYIDDAATLEVTFTMDVVPLITRLEKQFTSYQLKQVTQLTGKYAIRLYELLIAWREVG
                     NTPIFEVSDFRSKLGLTSDDYPRLDTFKRRVLESAVKQINEHTDIIVKVEQHKEGRSIS
                     GFSFSFKQKRNQNKAIERKKDPNTLDIFSRITDAQRHLFANKLSELPEMGKYSQGTESY
                     QQFAIRIAEMLQDPAKFQELHPYLEKVGYR"
                     /name="repM"
     gene            1005..1550
                     /locus_tag="OCUAc18_RS19550"
                     /old_locus_tag="OCUAc18_38320"
     CDS             1005..1550
                     /locus_tag="OCUAc18_RS19550"
                     /old_locus_tag="OCUAc18_38320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017398013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_005237874.1"
                     /translation="MKALSVIELSQLYGMNRQSIYKRINKGDLSKNSDGKIDLSEAIRV
                     FGEPSNKNNNVTPLQSTTVQKETEVDMLKQQVDILKKQLELAHEREVFQREQLEAKDNQ
                     IEAIQRLLEAPKTNMTTFTDQKPSQDIVTDPRSEPEPKHDGLTTPVETENKRIPVPEPV
                     EPEQPKRGWLSRFFLPNG"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(1752..2234)
                     /locus_tag="OCUAc18_RS19555"
                     /old_locus_tag="OCUAc18_38330"
     CDS             complement(1752..2234)
                     /locus_tag="OCUAc18_RS19555"
                     /old_locus_tag="OCUAc18_38330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005080650.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="WP_151708325.1"
                     /translation="MPNHKKIDKTDVKILELLQQDSRITTAQLSEELSLSTSPCWRRVK
                     QLEDTKIIKGYGVLLDRKKINLGVMVFIRVSIDSHSEKEAKKFEEEVTALDYVVACYSI
                     GGDADFLLQVVSPDLDTYAEFSMSIIRRLPGIKEMQSMFVLKEIKPFIGFPVKPSQ"
                     /name="Lrp/AsnC family transcriptional regulator"
     gene            2365..3000
                     /locus_tag="OCUAc18_RS19560"
                     /old_locus_tag="OCUAc18_38340"
     CDS             2365..3000
                     /locus_tag="OCUAc18_RS19560"
                     /old_locus_tag="OCUAc18_38340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004671195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="WP_151708324.1"
                     /translation="MDYLAQFTTYIFTLSLAAILPGPGMTGLMFKTLTQGYKNGLIMLI
                     GLITGDLIFLLTSIFLLTSLTQFFPNFSFYMVLLSSIYLLYLAYSFWSFNGDLLQTQAP
                     NTVNETIFSYRDGLLITLSNPKTISFYLAIVPAIFGAYSLKDQTFALMIITVLTLTVIG
                     GFYVFFSLGLKKFLRNLKIQRTLLKGLSLMMFILALSMIYREFTPHLT"
                     /name="LysE family translocator"
     gene            3130..4455
                     /locus_tag="OCUAc18_RS19565"
                     /old_locus_tag="OCUAc18_38350"
     CDS             3130..4455
                     /locus_tag="OCUAc18_RS19565"
                     /old_locus_tag="OCUAc18_38350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611903.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="WP_020899627.1"
                     /translation="MSDKELKRLSVLQEICDQRITQSQAAQLLHISERQIRRLLQKYKA
                     QGPSALAHAGRGQISNSKLPEELRLKCLNIVSDQLHGFGPTLAHEKLTTVHGFDLSVET
                     LRSWMIAADLWMPRSKRLKRPYQPRYNRDCFGELIQIDGSHHDWFEGRAAKCCLLVFID
                     DATGKLQHLRFCESESTFDYMISTRLYVEQHGKPLAFYSDKHSVFRVNQSSKKDTKITQ
                     FGRVLSTLNIDIIFANSPQAKGRVERANRTLQDRLIKEMRLKGICSIEQANVWLPCFIE
                     EFNQKFAKMAFNPKNLHRSITETAEELDDIFTWREPRRVTNSLTITYDKCVYLLENTEE
                     NQRLIGKYLEFLEYPDGTVAIMHNSRKINYSLFDKLSQLNQREIVENKRLGAVLNHIQQ
                     QHEELEQQNKRNRSQKMPSRRAQKTAIQERNLNPVLDLEMSI"
                     /name="ISNCY family transposase"
     gene            5053..6099
                     /locus_tag="OCUAc18_RS19570"
                     /old_locus_tag="OCUAc18_38360"
     CDS             5053..6099
                     /locus_tag="OCUAc18_RS19570"
                     /old_locus_tag="OCUAc18_38360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237878.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="WP_005237878.1"
                     /translation="MSVYGLWNNKGGVGKSYLTFQIAAEYAVQNPEKTVVVIDLCPQAN
                     SSMMLLGGVVDGYKNLESLKKNTISGYIEERIISPYIDPKKGYLYLTKVNDFNEKIEKN
                     LYLVAGDNSLELQADSIISSTRSGPGDAWQKVHLWIADLITSIKDNFKNQDTTFFIDFN
                     PSFSIYTTIGLSATDNLLIPFTADGSSLRAIRNVISLIYGSNKPGVPDSLFFQNSKQFR
                     MTIPKIYMYIGNRLTQANAGAASAFKSVINDITEEIWSLWKSDSSVFQTHPSNAPTPKN
                     ERLFKQMFLAEVNDANTASVYSGVTGIPMPLVTSGPSKNMNGKKVTINQSQLNKQIPNI
                     KDLVSKIV"
                     /name="ParA family protein"
     gene            complement(6183..7079)
                     /locus_tag="OCUAc18_RS19575"
                     /old_locus_tag="OCUAc18_38370"
     CDS             complement(6183..7079)
                     /locus_tag="OCUAc18_RS19575"
                     /old_locus_tag="OCUAc18_38370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4747 family protein"
                     /protein_id="WP_005237879.1"
                     /translation="MAGSRSCSVLYTAFDVQIFSDEVSTKTYVDLFLDLSGNLREVPLQ
                     ARSNYVKFDSIKPLNEDKLEHGFRCEIFKSTGLTSNFRDTVESQNVKLDTNRYIPTNYK
                     ANPKDFSAVFYPSERKIICELSFKKNKTAHAIVEEFLKKLVNTPTLNNKFKRIEITPIY
                     EKITADDINHNKTLTYVEAVINNIPDSFDDLNIVLSDKDRIFLKSLADSNLESYSQVLK
                     SKKAKFLELTDLNKDFIRLAIEYGFVKFHYKNIDNLTEKKSTLNISPFTKRLKIFPEDV
                     YGDFLIRETRKIAEQLS"
                     /name="DUF4747 family protein"
     gene            complement(7240..7410)
                     /locus_tag="OCUAc18_RS19580"
     CDS             complement(7240..7410)
                     /locus_tag="OCUAc18_RS19580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005237885.1"
                     /translation="MSNIIQLSKPCAFCDSRENVQLFSGLMLCGNCQENIRITNPGMFE
                     ANDQIEQKNQS"
                     /name="hypothetical protein"
     gene            complement(7407..7793)
                     /locus_tag="OCUAc18_RS19585"
                     /old_locus_tag="OCUAc18_38380"
     CDS             complement(7407..7793)
                     /locus_tag="OCUAc18_RS19585"
                     /old_locus_tag="OCUAc18_38380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005200047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005237886.1"
                     /translation="MNSTRTVAVRLDNQVAQEYERLAQLKGDKLGTYLRDLLTSNLQMA
                     SIERQANRIETLVNDFEKQINYSLDKFTEANYLNEKFFEDFGHIYMFQIGMLMHLKVDR
                     GDIQLMQTRGNAYAQRHYFAGEKP"
                     /name="hypothetical protein"
     Typing          2..936
                     /name="rep_cluster_1233"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.573"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgcctacca gtgaattaat agtaaaagat aatgctttaa ttaatgcgag ttataattta
       61 gataccgtag aacaacggtt aattctactg gctatcttac aagcaagaga aacaaaaaca
      121 gggattgatg caaacactag gctacggatc catgccagtg actatatgag cagatttaat
      181 gttagtaagc atgccgccta caaagcccta aaaacagcag ttactaacct ttttggtaga
      241 cagtttagct accaaacaat tgatgaaaaa acaggaaaat caaagaaagt aatttctcga
      301 tgggttcaaa atatttctta tattgatgat gctgcaacct tagaggttac ttttacaatg
      361 gatgtagtcc ctttaattac acgtttagaa aaacaattta caagttatca gttaaaacaa
      421 gttacccaat taacaggaaa atatgccatt cgactctatg agttgcttat agcatggcgt
      481 gaagtcggta acactcctat atttgaagtt tctgactttc gttcgaagct tggcttaact
      541 tcagatgatt acccaagact agatacattt aaacgacgtg tacttgaatc agcagtaaaa
      601 caaataaatg aacatacaga tatcatcgtt aaagttgaac agcataaaga aggtcgctct
      661 atttctgggt tctcttttag cttcaaacaa aaaagaaatc agaacaaagc aatagaacgt
      721 aagaaagatc ccaatacatt agatattttt tccagaataa cagatgctca acgccactta
      781 tttgctaaca agctttcgga attacctgaa atggggaaat actctcaggg cactgaaagc
      841 tatcaacagt ttgcaataag aattgctgaa atgcttcaag atcctgctaa gtttcaagaa
      901 ttacatcctt atcttgaaaa agttggctat cgttaaacta ctgatgtaac gttacatgat
      961 taattattgt ctaaaagcat acagattttt acttgggtta cataatgaaa gcactatctg
     1021 ttattgagtt atcacaactt tatgggatga atcgacaaag tatttataag cgtataaata
     1081 aaggagattt atcaaaaaat agtgatggga aaatagattt atctgaagcc ataagagtat
     1141 ttggcgaacc atctaataaa aataataatg taaccccgtt acagtctact acggtacaaa
     1201 aagagacaga agtagacatg ctaaaacagc aggtagacat actaaaaaaa cagctagaac
     1261 ttgcacatga gcgagaagtt ttccaacgag aacagctaga agcaaaagac aatcaaatag
     1321 aagctataca acgactgcta gaagccccaa aaaccaatat gactacgttt accgatcaga
     1381 aacctagcca agatatagta acggatcctc ggtcagaacc tgagccgaaa catgacggat
     1441 tgactactcc ggtcgaaaca gaaaataagc gtattccagt tccggagccg gttgaaccgg
     1501 agcagccgaa aagaggatgg ttaagccggt ttttcttgcc gaatggctaa gaggcttgtt
     1561 taggtgtact aatcaaagct gtggaacttt gatcaaagaa taggcgtgga acgtgaaaaa
     1621 tcataaaaaa agcccgaaca gggacgttcg ggttataaac tagaaactac agcattgatt
     1681 tctatagata attataacgc atttcgtata aggtgtatta tgttaatttt aggaaaactt
     1741 aacttagaat actattgaga aggcttcact ggaaaaccta tgaatggttt tatttctttt
     1801 aaaacaaaca tactctgcat ttcttttatt cccggcaatc ttcttattat tgacatagag
     1861 aattcagcat aagtatcaag atctggggat acaacttgaa gtaagaaatc tgcatcacct
     1921 ccaatgctgt aacatgcaac gacataatct aaagccgtca cttcttcctc aaatttttta
     1981 gcttcttttt cactatggct atcaatagat acacgtataa ataccattac acctaaattt
     2041 atttttttac gatcaagtaa tactccatat cctttaataa tcttagtatc ttcaagttgc
     2101 ttgactctac gccaacatgg agaagttgat aagctcaatt cttctgataa ctgggctgtt
     2161 gtaatacgtg agtcctgttg caacaactcc aaaattttta catcggtttt atcaattttt
     2221 ttgtgattag gcataaatac taaaattatt aaataaaata gaaattttat acccttattt
     2281 atcaatttta taaaaaatat agatcaattt cttctttgtt tttactaaaa aatagtctca
     2341 attcttttat tgatttacct tcaaatggac tatctagcac aattcaccac atatattttc
     2401 actttatctt tagccgcaat tcttcctggg cctggtatga caggtctaat gtttaaaaca
     2461 ctgacacagg gctataaaaa tggcttgatt atgctaattg gtctaatcac aggtgatcta
     2521 atctttctat taacctctat ttttttactg acttctttaa ctcaattttt tccaaatttt
     2581 tctttttata tggtcctact atcgagtatt tatttattgt atttagctta tagtttttgg
     2641 tcttttaatg gtgatctgct acaaactcag gctcctaata cggtaaatga aactatattc
     2701 tcttatagag atgggctatt gataacactc agtaatccta aaaccatttc gttttaccta
     2761 gcaattgttc cagctatttt tggagcatat agtctgaaag atcaaacttt tgccttaatg
     2821 ataattacag ttctgacttt aactgttatt ggcggttttt atgtattttt ttctttaggc
     2881 ctaaaaaagt ttttacgtaa tttgaaaatt caaagaactt tgcttaaggg actatctctt
     2941 atgatgttta tcttagctct aagcatgata tatagagaat ttacgcctca tttaacataa
     3001 tggcggttat gcgaaatcaa aatgtaactc caaaatagaa atgtccggtt tctccaaagt
     3061 aaaaatgtcc gcttttagaa tatgcacttc tgcaatttcc ttagcggacg gtttgatatg
     3121 ttggtgtcta tgtcggataa agaacttaaa cgattgtcgg tcttgcagga aatctgcgat
     3181 caacgcataa ctcaatccca ggctgctcag ctacttcata tttcagaacg tcagatcaga
     3241 cgcttactgc agaaatacaa agctcaaggt ccatctgcat tagcccatgc cggtcgtggc
     3301 caaatcagca attctaaact tcctgaagaa ctcagactca agtgcctcaa tattgtttct
     3361 gaccaactgc atggtttcgg acccacttta gcgcatgaaa agcttaccac cgtacatgga
     3421 ttcgatcttt cagtagaaac cctgcgttct tggatgattg cagctgactt atggatgcct
     3481 agatccaagc gcctgaaacg accgtatcag cctcgttaca accgggattg ctttggtgaa
     3541 ctgatccaaa ttgatggctc acaccatgac tggtttgaag gacgcgctgc taaatgctgt
     3601 ctgctggtgt ttatcgatga tgctacagga aaattgcagc atttacgctt ctgtgagtca
     3661 gaatcaacct ttgactatat gatttcaaca cgcttatatg tcgaacagca cggtaagcct
     3721 ttagcgtttt acagcgacaa acattcagtc tttagagtga atcaaagcag caagaaagac
     3781 accaagatta cccagtttgg acgtgtactc agcaccctga atatcgatat catcttcgct
     3841 aattcaccgc aggccaaagg ccgtgtggaa cgggccaaca gaacccttca ggaccgtctg
     3901 atcaaggaga tgcgcctgaa aggcatctgt tcgattgagc aagctaatgt ctggctaccc
     3961 tgcttcattg aggagttcaa tcagaagttc gccaagatgg cttttaatcc taagaatcta
     4021 catcggtcta tcactgaaac agccgaagaa ttagatgata tttttacttg gcgtgaaccc
     4081 cgcagagtca cgaatagtct gacgattact tatgataaat gcgtatatct cctggaaaac
     4141 accgaagaaa atcaaaggtt gatcggtaag tatcttgagt tccttgaata cccggatggt
     4201 actgtagcca tcatgcataa tagccgaaag atcaattaca gcctcttcga taaattaagt
     4261 cagctgaatc agcgagagat tgttgagaat aaacggttgg gtgctgttct gaatcatatc
     4321 caacaacagc atgaagaact ggaacagcaa aacaaacgta atcgttctca aaagatgcca
     4381 agcagacgtg cacagaaaac agcaattcaa gaacgaaatc taaatcctgt gcttgacttg
     4441 gaaatgtcca tataggacat ttctatttgg ttattaggta ggacatttct acttaggaat
     4501 aacagcgatt taaaatagtt ttatatttca aaaagttatg atataaaaaa atgggacgca
     4561 aaacctaaaa agccgacttg gaaacaagtc ggctttttta tgagcagatt tgatacttct
     4621 cgccaataaa accggttaat atttaatcaa aatcaagctt tagtgtgtgt tatcaatcct
     4681 ctcaggactt aaatcatccc caatttctgc ggacaactct tgggataaaa ataggccaac
     4741 tttgtaagct aaaacttaca taagtttaag gattttgcgg attggtaatt caaggcatta
     4801 atcctatgat tgctatatag ggaacaggat gtttccagaa aaacaagaat aagaaagtag
     4861 cgcaaggaat gggcagcacg agatcagcaa gacgagcaac ccaatctaga aaagcccgac
     4921 aggatgtcgg gcttttttac tttaagtaac gtgcttttat gttaaaaaca ttctaattcc
     4981 taaagaaaga gtaacagtac taattttaaa tacattacta tgcgttaaag tttaatcttc
     5041 tgagaaattg ctatgtcagt atatggttta tggaataaca aaggcggtgt agggaaaagc
     5101 tatttaacat tccaaatagc tgcagaatat gctgtccaaa atccagaaaa aactgtagtt
     5161 gtcatcgatc tatgtcctca agcgaattca tcaatgatgc tattgggcgg tgtagtagat
     5221 ggatataaaa atcttgaatc gcttaaaaag aatacaattt ctggttatat tgaagagaga
     5281 attattagtc catacatcga ccctaaaaag ggttatcttt atctgacaaa agtcaatgat
     5341 ttcaatgaaa aaattgagaa aaacttgtat ttagtcgcag gcgataattc tttagaatta
     5401 caggcagata gtattatttc ttctacgcgt tctggtccag gagatgcttg gcagaaagtg
     5461 cacttatgga ttgctgatct tatcacgagt attaaagata attttaagaa tcaagatact
     5521 acttttttta tagactttaa tccgagcttt tctatctata cgactattgg tttatctgcc
     5581 acagataatt tattgattcc atttactgcc gatggatcat ctctacgagc tattagaaat
     5641 gtgatttcac taatatatgg atcaaataaa cctggtgtac cagattcatt attcttccaa
     5701 aatagtaaac aatttagaat gactattcct aaaatttata tgtatatcgg taatagatta
     5761 acgcaggcta atgctggtgc tgcaagtgca tttaagtcag taataaatga tataactgaa
     5821 gagatttgga gtttgtggaa gtcggactct tctgtatttc agactcatcc atcaaatgca
     5881 cctactccaa aaaatgagag actatttaaa caaatgtttt tagcagaagt taatgatgct
     5941 aatacagcat ctgtatattc aggagttaca ggtattccta tgccattagt tacttcaggg
     6001 ccttctaaaa atatgaatgg taaaaaagta actattaacc aatcacagct gaataaacaa
     6061 atacctaaca tcaaagactt agtttctaaa attgtttaat tagtaaattt atagccaaca
     6121 tcttgtaaat gttggctttt ttttaatttc atttagcata gcatcatgta aaatcttaag
     6181 tcttaagaaa gttgttctgc tatttttctc gtttctcgta ttaagaaatc tccataaacg
     6241 tcttctggaa aaatttttaa acgttttgtg aatggtgaaa tgttaagcgt gcttttcttt
     6301 tctgttagat tatctatatt tttataatga aattttacaa atccatattc tatggctaat
     6361 ctaataaaat ctttatttag atcagttagt tctaaaaact ttgccttttt acttttaaga
     6421 acctgagaat aggactctaa gttactatcc gccagacttt ttaagaaaat tctatcttta
     6481 tcagataaaa ctatatttag atcatcaaaa gaatcaggaa tattatttat aactgcttca
     6541 acgtaagtca atgttttatt atgattaata tcatcagccg taattttttc ataaattggc
     6601 gttatttcaa ttcgcttaaa tttattattt agtgtaggtg tattaactaa ttttttaaga
     6661 aattcttcaa caattgcatg cgctgtttta ttctttttaa aagataattc acaaataatt
     6721 tttctttctg atggatagaa aacggcacta aaatctttag ggtttgcttt atagtttgtc
     6781 ggaatatatc tattagtgtc taattttaca ttttgagatt ctaccgtgtc tctaaaatta
     6841 ctagtaagcc ctgtactttt aaagatctca catctaaagc catgctctaa tttatcttca
     6901 tttaaaggct ttatactgtc aaactttacg taattggaac gagcttgcaa aggtacttca
     6961 cgaaggttcc cacttagatc caaaaacaaa tctacatatg tctttgttga aacctcgtca
     7021 gaaaaaatct gtacatcaaa agctgtataa agaacagaac atgaacgtga acctgccata
     7081 tttctctcat taatattatc gagcaataat tatacattta ttgtaaatta aatataatct
     7141 tcttcttctt tttcataatc atataagttg ttacaacttg tataattaat ccaaagaatt
     7201 tttagtaaat aaaaatagag tactgtatta aaaaccagat taactctggt ttttttgttc
     7261 aatctgatca ttggcctcaa acatgccagg attggtaatc cggatatttt cctgacaatt
     7321 cccacaaagc atcagccctg aaaaaagctg tacgttttcc cttgaatcac agaaagcaca
     7381 tggtttagag agttgaataa tattgctcat ggcttttccc ccgcaaaata gtgcctttga
     7441 gcatatgcat ttcctctagt ttgcattaat tggatgtctc ctcgatcgac ttttagatgc
     7501 atcagcatcc caatttgaaa catatatata tggccaaagt cttcaaagaa tttttcattt
     7561 aaataattgg cctcagtaaa cttatcgagg gagtaattaa tttgcttctc aaaatcattc
     7621 accaatgttt caatccgatt ggcctgccgt tcaatactgg ccatctgtaa attactggtc
     7681 agaagatccc gcagataagt ccctaattta tcgcccttca actgagccaa acgctcatat
     7741 tcttgtgcga cctgattgtc taatctgacg gcaacggttc gagtactgtt catgaaaaac
     7801 tcctacaatt tgttttcaat ctaagcacct aatttttaaa aagtaactat caaatataag
     7861 taaacactgt tttaagtttt aatgattaca ggttaaatat ctaatataaa aagagaaatt
     7921 tacataaaaa cgtaaacaaa ggtgattaca agctatctca ttgttttgta agtaaatatt
     7981 gcactttatt tgcaacaaaa catgtttaca gctaaaccta tgaaaaataa taaaaatcat
     8041 gctaaaagcg taaacgcttt ccagatagat gaaaaatcgc tgtatccctt ataggataag
     8101 gctttggagc ctttatcttt agatgcgtgg tgtgtgaacc aaaaattgcc ggtatgtacc
     8161 ggctcctaat ccatttaatt gacttaactg acagtaaaga tagtgcttgg ccaaaagaga
     8221 tcctgactat tgtattttta agagaaaaat ctataagcga aaactctagc ctacagatca
     8281 aaaataaaac ctaagttaaa acgctctcac atgcgatttc agccacattt ttacataaga
     8341 catagaatca ctattgaaat gagtaatcag atcaaaaaac gcaaatgagc gccaaataga
     8401 gactattttt attctaatcg ggatgaatag agcgagtgtc tacgagcgaa gcaccaaaat
     8461 tcgcacctcg actctctgaa aaacagcttt ttaggctgtg aagcctaaat aagcgtaaat
     8521 tacatgcaga aaattgggcc tagatcgagc gtagcgagta aaaagcttta tgagcaaagc
     8581 gaattccgag tttcttttgc tcttgccttt gctttttctc aaagtcacaa ctgaaagagc
     8641 taaaaaattg ccccacgaat cgagcgaaag cgagattcaa tagagtttga gcgtagcgaa
     8701 aaccaagggc aatttttcat tctcaatgct tttaataatt tttaagtttt taaatgcttt
     8761 tagatctgct gtaagccttt atttatagta tatacaagag atataccaca ccaaaaagtc
     8821 acctatacca caccaaaaag tcacctatac cacaccaaaa agtcacctat accacaccaa
     8881 aaagtcacct atttacacca ttgattaatg atggtgtaat aacaccacgt ataataaagg
     8941 tgtagcaa
//
