LOCUS       NZ_AP024805            10831 bp    DNA     circular CON 21-APR-2024
DEFINITION  Acinetobacter baumannii strain OCU Ac18 plasmid pOCUAc18-3, complete
            sequence.
ACCESSION   NZ_AP024805
VERSION     NZ_AP024805.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00330123
            Sequence Read Archive: DRR301936, DRR301937
            Assembly: GCF_019703285.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1
  AUTHORS   Sakiyama,A., Matsumoto,Y., Tsubouchi,T., Suzuki,M., Niki,M.,
            Niki,M., Oinuma,K.I., Kakeya,H. and Kaneko,Y.
  TITLE     Complete Genome Sequence of a Clinical Isolate of Acinetobacter
            baumannii Harboring 11 Plasmids
  JOURNAL   Microbiol Resour Announc 10 (39), e0069521 (2021)
   PUBMED   34591663
  REMARK    DOI:10.1128/MRA.00695-21
REFERENCE   2  (bases 1 to 10831)
  AUTHORS   Tsubouchi,T., Sakiyama,A., Matsumoto,Y., Suzuki,M. and Kaneko,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUL-2021) Contact:Taishi Tsubouchi Osaka City
            University, Bacteriology; Abeno, Asahimachi 1-4-3, Osaka, Osaka
            545-0051, Japan URL :http://www.med.osaka-cu.ac.jp/bacteriology
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Illumina Novaseq; ONT Flongle
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019703285.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 04:56:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,865
            Genes (coding)                    :: 3,655
            CDSs (with protein)               :: 3,655
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 210
            CDSs (without protein)            :: 210
            Pseudo Genes (ambiguous residues) :: 0 of 210
            Pseudo Genes (frameshifted)       :: 58 of 210
            Pseudo Genes (incomplete)         :: 134 of 210
            Pseudo Genes (internal stop)      :: 38 of 210
            Pseudo Genes (multiple problems)  :: 17 of 210
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP024805.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10831
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="OCU Ac18"
                     /isolation_source="venous blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="pOCUAc18-3"
                     /geo_loc_name="Japan"
                     /lat_lon="34.68639 N 135.52 E"
                     /collection_date="2016-07-16"
     gene            1..909
                     /gene="repM"
                     /locus_tag="OCUAc18_RS19460"
                     /old_locus_tag="OCUAc18_38140"
     CDS             1..909
                     /gene="repM"
                     /locus_tag="OCUAc18_RS19460"
                     /old_locus_tag="OCUAc18_38140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680120.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_026441244.1"
                     /translation="MAELVVKDNALIQASYTLDTVEQRLILLAIAEARETGQGITENSL
                     LEVHASSYINTFNVEKHTAYTVLRDASRSLFDRYVTYHDINPKTGKDRSFHCRWVDKIG
                     YEHQSGVVFLRFTQDIVPLITRLEENFTKYEIQQVSKLTSTYAIRLYELLIQWRSSGQT
                     PVFNLSTFRQQLGVEATQYKTMSNFKTYVLDFALKQVNELTDITANYQQHKTGRTISGF
                     SFTFKQKQERKKVKPKKASLTEKQLDLFANKLAYDSAFSSQFSEAGESYENFAKRIKQL
                     LKDPVNLDKYSDYLDNLDLKV"
                     /name="repM"
     gene            981..1502
                     /locus_tag="OCUAc18_RS19465"
                     /old_locus_tag="OCUAc18_38150"
     CDS             981..1502
                     /locus_tag="OCUAc18_RS19465"
                     /old_locus_tag="OCUAc18_38150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017397202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_026441245.1"
                     /translation="MKSLSVVELSKLYSINRQTIYNHINKGILSKNSDNKIDLSEAIRV
                     FGEPSKKQSVKEPVKVDSQNSTENLLLRQQIDMLKNQLEDAKERESFYQNQIETMQRLL
                     EAPKANMTTFIDQKREQNIATDPQKEPELKADELTTPVETENKRIPVPEHIEPEPEKRG
                     FWSRFFRPYD"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(1702..1938)
                     /locus_tag="OCUAc18_RS19470"
                     /old_locus_tag="OCUAc18_38160"
     CDS             complement(1702..1938)
                     /locus_tag="OCUAc18_RS19470"
                     /old_locus_tag="OCUAc18_38160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016659738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_110975945.1"
                     /translation="MNDQIFDAIREALANADESSWTQELHIQVIKYADSLADVTGKEFC
                     EAVQIKDSYFAEFNKMKKIATRLIAAGLDVSRL"
                     /name="hypothetical protein"
     gene            complement(1965..2534)
                     /locus_tag="OCUAc18_RS19475"
                     /old_locus_tag="OCUAc18_38170"
     CDS             complement(1965..2534)
                     /locus_tag="OCUAc18_RS19475"
                     /old_locus_tag="OCUAc18_38170"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016659739.1"
                     /translation="MNNQKIEHQSVIVFTSESKDSLVKNGGSRAWRAVLSKLEQAEYLI
                     CTQNTNKLHEHDPEIDHGQAFYIGKVRNIEIVEDDRKFIQVSEYAFLPNDVVFKEAWKR
                     LTQGESNQAQQYPVAYKDTNELFEMLHLNLDELDWITVDQTQRINLNEPKTITSVNLPE
                     LIEEARQKISEAANVSPDKVTIQISF"
                     /name="hypothetical protein"
     gene            complement(3185..3457)
                     /gene="nadS"
                     /locus_tag="OCUAc18_RS19480"
                     /old_locus_tag="OCUAc18_38180"
     CDS             complement(3185..3457)
                     /gene="nadS"
                     /locus_tag="OCUAc18_RS19480"
                     /old_locus_tag="OCUAc18_38180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="WP_000369780.1"
                     /translation="MDNNLFDDLVASIKEAGAIKRKEIKASRVTELELPDIKEVREKTG
                     LTQAEFAARLHISARTLQNWEQGRRYPTGPAATLIRILDAHPTLI"
                     /name="nadS"
     gene            complement(3450..3770)
                     /locus_tag="OCUAc18_RS19485"
                     /old_locus_tag="OCUAc18_38190"
     CDS             complement(3450..3770)
                     /locus_tag="OCUAc18_RS19485"
                     /old_locus_tag="OCUAc18_38190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000897310.1"
                     /translation="MLFIETSIFTKQIKELVSDEEYRQLQQDLLVQPDKGNLIKNGGGI
                     RKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAYPKSVKDNLTDKETSILRQLVKEQF
                     HG"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3957..4154)
                     /locus_tag="OCUAc18_RS19490"
                     /old_locus_tag="OCUAc18_38200"
     CDS             complement(3957..4154)
                     /locus_tag="OCUAc18_RS19490"
                     /old_locus_tag="OCUAc18_38200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000476222.1"
                     /translation="MFKQTLSVKDQFGVKHAIQATVDHEFANTQSHLNIKHITVDGEDI
                     RPSFEMLFQSTLSGKIFKII"
                     /name="hypothetical protein"
     gene            complement(4221..4448)
                     /locus_tag="OCUAc18_RS19495"
                     /old_locus_tag="OCUAc18_38210"
     CDS             complement(4221..4448)
                     /locus_tag="OCUAc18_RS19495"
                     /old_locus_tag="OCUAc18_38210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005166620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000921865.1"
                     /translation="MLKVNDLIAKSKNGTEIIVSLIPLHKMQNTRQGLKQIEVGKRVLL
                     ESGIEVDLNLDGRTFYTSLNQLFKLNHKVA"
                     /name="hypothetical protein"
     gene            complement(4546..4761)
                     /locus_tag="OCUAc18_RS19500"
                     /old_locus_tag="OCUAc18_38220"
     CDS             complement(4546..4761)
                     /locus_tag="OCUAc18_RS19500"
                     /old_locus_tag="OCUAc18_38220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004693381.1"
                     /GO_component="GO:0005829 - cytosol [Evidence IEA]"
                     /GO_function="GO:0003676 - nucleic acid binding [Evidence
                     IEA]; GO:0003723 - RNA binding [Evidence IEA]; GO:0001072 -
                     transcription antitermination factor activity, RNA binding
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="WP_000071892.1"
                     /translation="MSNSNVVKGTVKWFNETKGFGFIQQESGPDVFAHFSEIASSGFKT
                     LFEGQQVEFSVAQGQKGPNAVNIVAI"
                     /name="cold-shock protein"
     gene            complement(5117..5512)
                     /locus_tag="OCUAc18_RS19505"
                     /old_locus_tag="OCUAc18_38230"
     CDS             complement(5117..5512)
                     /locus_tag="OCUAc18_RS19505"
                     /old_locus_tag="OCUAc18_38230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family toxin"
                     /protein_id="WP_004759377.1"
                     /translation="MIYLLDTNICIYVINNKPQHVFERFKQYQLGQLAISSITASELAF
                     GVEKSGSERNKQALNKFLSPLEILPYDEQAIWHYAKLRQDLQSTGKPIGSLDMLIAAHA
                     LALDVVLVTNNIKEFERIDGLKLENWV"
                     /name="type II toxin-antitoxin system VapC family toxin"
     gene            complement(5509..5739)
                     /gene="vapB"
                     /locus_tag="OCUAc18_RS19510"
                     /old_locus_tag="OCUAc18_38240"
     CDS             complement(5509..5739)
                     /gene="vapB"
                     /locus_tag="OCUAc18_RS19510"
                     /old_locus_tag="OCUAc18_38240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005155998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="WP_004282079.1"
                     /translation="MEIAKVFQTGRSQAVRLPKAFRFNGTEVAIKSFGRGVLLMPIDNP
                     WDVMLEAVNEFESGFQLERADQGEQFREEFK"
                     /name="vapB"
     gene            6370..7653
                     /locus_tag="OCUAc18_RS19515"
                     /old_locus_tag="OCUAc18_38250"
     CDS             6370..7653
                     /locus_tag="OCUAc18_RS19515"
                     /old_locus_tag="OCUAc18_38250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004774628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_151838536.1"
                     /translation="MIDGKTLPLRTDVPENDLETFENKLLNLCQEYFLDLSKSELLQTG
                     RSKDILEKYKENKSLSNFEQAYINEYLLRSLTQFLPSFLKRTANKVELERFTEEKVRQE
                     KLRQEAIKKQKLLESDPVYIAKQKEQALLKKYEINEYISGTLPKEISEILSKLEGGIRL
                     SQDEAVWLNTIGKRFFSSKVRHKFHRLEANHYLQEFEKNKKNIWNAINASSQLRKCQAS
                     LEAEELLEKISIAGIKDKKLLSAYFTTLGGVRRDLRKVEVAIDNASKAHTLTPDNYRPC
                     TLLGAIYMETRQYTLGHEWYEKASERGAPKQSINADLKSILSKMDKAQRNEMIEHLLKL
                     DSYTYSWLKSLKAAVVNNPKSKQDKVEQPVKAQKKASPSQSKKNVQNKQPKNSEKSLQN
                     LLEQGAKAHNLKQPVNLSKSSCMKPKKK"
                     /name="hypothetical protein"
     gene            complement(7703..7924)
                     /locus_tag="OCUAc18_RS19520"
                     /old_locus_tag="OCUAc18_38260"
     CDS             complement(7703..7924)
                     /locus_tag="OCUAc18_RS19520"
                     /old_locus_tag="OCUAc18_38260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005244543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_087548429.1"
                     /translation="MKNPVDADRIRAAFKATQGIVEHEFEQHVIDASQVYVDAMIERIA
                     SGESTFEEEDEVLMMKWRAMNALQDKDS"
                     /name="hypothetical protein"
     gene            complement(7921..8277)
                     /locus_tag="OCUAc18_RS19525"
                     /old_locus_tag="OCUAc18_38270"
     CDS             complement(7921..8277)
                     /locus_tag="OCUAc18_RS19525"
                     /old_locus_tag="OCUAc18_38270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_221255522.1"
                     /translation="MKTGKQILEGLFDDQRAAAHDPHHPVNDQLRASLLWKKHLLELIQ
                     IPRHQLKSDDSYVLVSDIPEYFKAQFEQFFLGQNLLQHVDHPDESRLDSKRLDSFIAYL
                     KDHPSYQSEQRDQV"
                     /name="hypothetical protein"
     gene            complement(8317..9207)
                     /locus_tag="OCUAc18_RS19530"
                     /old_locus_tag="OCUAc18_38280"
     CDS             complement(8317..9207)
                     /locus_tag="OCUAc18_RS19530"
                     /old_locus_tag="OCUAc18_38280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005244541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_221255523.1"
                     /translation="MFYMNIRHNVKKDSSSKNTFHYLTRTAHFAEQKDDEELEFVRSGN
                     MPAWAVDAPATFWHSADRYEIARGRTSSTLTVALPKELSKQQRKELVEAFIQEFADQYQ
                     FPYTAAVHNHPSELTGEDQPHLHLMYSERSLADGIERPPEQFFKQYRPKNPTKGGAQKL
                     TADALGFGRDQVKVFREKTEQLINQALEMHAPMKTVILEGMEIVVPNRVSHLSNQEFNQ
                     KYGTQLQDVPQIYRWKLKSADPMIQLEVEAQKQTIQQIRQFNKLQIYQKEYNQALEQRK
                     NQDLDRDDGYTPSWF"
                     /name="MobA/MobL family protein"
     gene            9419..10141
                     /locus_tag="OCUAc18_RS19535"
                     /old_locus_tag="OCUAc18_38290"
     CDS             9419..10141
                     /locus_tag="OCUAc18_RS19535"
                     /old_locus_tag="OCUAc18_38290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017724910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_221255524.1"
                     /translation="MAGRKALVLTAKEINELGSHILNLPFKRRVEERCLHMLKNKKSLQ
                     DLSEQDRQLIQKCRYERNAYNKRMLQLQLIQQTAPNKQTALEQNILKLHQKHDIDAYFA
                     MHDALDEILKTQRHQTAAKNLNQKIEKALNPEQQKEKQSQKQQKKREDQIKYFIGSLYL
                     GVFERAKFQMTHSNQDLDNLKTLFRMALIGKTMQQSNKDLQTVTQEIANSSQYQEIERF
                     IREAKQDPRNPFNKTPEQ"
                     /name="hypothetical protein"
     gene            10388..10582
                     /locus_tag="OCUAc18_RS19540"
                     /old_locus_tag="OCUAc18_38300"
     CDS             10388..10582
                     /locus_tag="OCUAc18_RS19540"
                     /old_locus_tag="OCUAc18_38300"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_151809600.1"
                     /translation="MQKFEPRPSEASIKLYERSEFRIAFALAFAFSQIHNEKNQKIAPR
                     IERKRDSIEFERSENQGQF"
                     /name="hypothetical protein"
     Typing          complement(8321..9198)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.587"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggccgagt tagttgtaaa agataatgct ctgattcaag caagttatac attagatact
       61 gttgagcaac gtcttattct tttggcaata gctgaagcaa gagaaacggg acaaggcatc
      121 acggaaaata gcttgttgga agttcacgct agtagctaca taaatacttt taatgttgaa
      181 aaacatactg catataccgt tcttagggat gcctctagaa gcttatttga tcgctatgtt
      241 acttatcacg atataaatcc aaaaacaggt aaagacagaa gtttccactg tagatgggtg
      301 gataaaattg gttatgaaca tcaatcagga gttgtattct tacgtttcac tcaagatatt
      361 gtccctctaa ttactagatt agaagaaaat tttactaaat atgaaataca acaagtttcc
      421 aaattaacaa gtacatacgc cattcgctta tatgagctac taatccaatg gcgatctagt
      481 ggccaaacac cagtttttaa tctatcaaca ttcagacaac aactaggcgt tgaggctact
      541 caatacaaga ctatgagtaa ttttaaaacc tatgtcctag attttgcctt gaaacaagtt
      601 aatgaactaa cagatataac ggctaactat caacaacata aaactggcag aacaatttct
      661 ggtttctctt tcacattcaa acaaaaacag gaacgtaaaa aagttaagcc taaaaaagct
      721 agcctaacgg agaaacaatt agatttattt gcaaataagt tggcttatga ctctgctttt
      781 tctagccaat ttagtgaggc tggtgaaagc tatgaaaact ttgccaaaag gattaaacaa
      841 ctgttaaaag accctgtaaa tttagacaaa tattcagatt atttagacaa tttagattta
      901 aaagtttagg ctataaaggc tgtctaatag acagcctatt agacatatta tttttacaag
      961 attaggcata gttttacaca atgaaaagtc tttctgttgt tgagttatct aagctatata
     1021 gcataaatag acaaactatt tacaaccata taaataaagg aatattaagc aaaaattcag
     1081 ataataaaat tgatttatct gaagcaatcc gtgtgttcgg tgaaccatct aaaaaacaaa
     1141 gtgtaaaaga acctgtaaaa gtagacagtc aaaattcgac agaaaattta ctgcttagac
     1201 aacaaataga catgttaaaa aatcaacttg aagatgctaa agaacgtgaa tctttttatc
     1261 agaatcaaat tgagacgatg caacgtctac tagaagcccc aaaagctaat atgactacgt
     1321 ttatcgatca aaaacgtgag cagaatatag caacagatcc tcagaaagaa cctgagctga
     1381 aagctgatga attgactact ccagttgaaa ctgaaaataa gcgcattcct gtgcctgaac
     1441 acattgaacc agagcctgag aaaagagggt tttggagtcg tttttttaga ccctatgatt
     1501 aatcaagatt gtttaatttg aatgaataaa atcacggaac aatgagcaaa gaataggcgt
     1561 ggaacgtgaa agatcaagaa aaagccgttc cagtgcgctg aagcctagaa cggcttactt
     1621 taagatattg atgcgaaaag gaaaagttca agtatttcgc ataaggcgta ttatgttaat
     1681 tttagataat ttaaataaag gctagagacg actaacatct aatcctgctg cgattaatct
     1741 tgttgctatc tttttcatct tattaaattc agcaaaatag ctatccttaa tttggacggc
     1801 ctcacaaaat tcttttcctg tgacatcagc caaactatct gcatatttaa taacttggat
     1861 atgtaattcc tgtgtccaag aactttcatc tgcatttgct aaagcttcac ggatagcgtc
     1921 aaaaatttga tcattcatct tgattccttc tagatttatt tactttaaaa tgaaatttgg
     1981 attgttactt tatctgggct tacattggca gcctctgaaa ttttctgcct ggcttcctct
     2041 attaattcag gcaagtttac agatgtaatt gtctttggct catttaaatt aatacgttgt
     2101 gtttgatcta cggtaatcca gtccaattca tctaagttca aatgcaacat ttcaaataac
     2161 tcattagtat ccttatatgc tacgggatat tgttgggctt gatttgattc gccttgcgtc
     2221 aaccgcttcc atgcttcttt aaatacaaca tcattcggta aaaatgcata ttcagaaact
     2281 tgtataaatt ttcgatcatc ttcaacaatt tcaatattcc gaaccttacc aatataaaaa
     2341 gcttgtccat gatcaatttc aggatcgtgc tcatgtagct tattcgtatt ctgagtacat
     2401 attaaatatt ctgcttgttc taatttactt agtacagctc gccaagctct acttccacca
     2461 ttcttcacta aagaatcttt cgactcagat gtaaagacaa ttacagattg atgttcaatt
     2521 ttttggttat tcatgacgta tatcctataa atattggttt taaaatttgt atatcgagtc
     2581 tcaataagcc aaatatacag atatacaata cgaatatcaa tacatacgca gagaatattt
     2641 ttaaaaatta caaaaattac gaaatataca tagagaatat ttttaattat atgaatatac
     2701 attccattta acataatggt gcttatgcga aatggccctt taagtttact ttaagatcaa
     2761 taacttacat tgagttaaac ccctggaacg atctcgttca accgatttag aaataaatcg
     2821 gtttttttat gttcaatttt gatacttctt gccaataaaa ttgtttaaaa aataatcaaa
     2881 aatagcaatt ccatagagtt tataagcttt tttacccaaa agaatccact atttctgtgg
     2941 ataaccactg ggataaattt tgattatttt ttgtacgttt tggcttacag cgtttcacga
     3001 aattgtctgt atctatcccc cgatgaagct cttatgatcg atctatatgg acaggatgtt
     3061 caatgactta aaacaacaat aataatcagt caggcaccat gaccatcagg tagtcagccc
     3121 tacctatgcg ctccataccg aaaaatcccg acatgacgtc gggatttttt attatctaaa
     3181 cttcttaaat taaagttgga tgagcatcta aaatacgaat taaagtcgct gcaggaccag
     3241 tcggatagcg acggccttgt tcccagtttt gcagggttct cgcgctgata tgcaaacgtg
     3301 cagcaaattc agcctgggtt aagcctgttt tctcacgtac ttctttaata tccggtaatt
     3361 caagctctgt gactcgactt gcctttatct ccttacgctt gatggcacca gcttctttga
     3421 ttgaagcaac caggtcatca aataagttgt tatccatgaa attgctcctt tactaattga
     3481 cgtagaatcg aggtttcttt gtctgtcaga ttatctttca cagactttgg ataagccacc
     3541 aggaaaaaga tctgatcatc ttctgtaacc caataataga tcacacggat accgccactt
     3601 ttgcctttat tgccctgagc acaacgtact ttacgaattc caccaccatt cttgattaag
     3661 ttgcccttat caggctgtac caagagatct tgctggagct gacgatactc ttcatcactc
     3721 acaagctctt tgatttgctt agtaaaaata ctggtttcaa tgaataacat aggtttaagt
     3781 acgtcaatgg cgtataagaa ttttagcagt tttagaataa aaaaacgaga gctgtgaact
     3841 ctcgtttcgc ataagcaaag gcttacaaat ataaatatta ggtataaaaa acccgcatga
     3901 tagcgagtag gtcctgatat ttcattcaga ctatagattt tataaaaact atatttctag
     3961 atgattttaa atatttttcc acttaaggta ctttgaaaaa gcatttcaaa actaggtcga
     4021 atatcttcac catcaacggt gatatgtttt atattcaaat gagattgtgt gttggcaaac
     4081 tcatgatcca ctgtggcttg aatagcgtgt ttaacgccaa actggtcttt tacgctgaga
     4141 gtctgtttaa acatatatat ttccttaaaa aaattgcagt ggaaatccca cataaattat
     4201 tgatgtttaa aaatttatcg ctaagcaact ttatgattta atttaaatag ctgatttaga
     4261 gaagtgtaaa atgtacggcc atccaaattt aaatcaactt cgattccaga ttccagtaga
     4321 actcttttac ctacttcgat ttgttttaaa ccttgacgtg tattttgcat tttatgtaga
     4381 ggaattaatg aaacaataat ttcagtacca tttttagatt ttgcaattaa atcatttact
     4441 tttaacaact tatatcctat tttttagaga ttttttcaat tttgaaaaca ttttaaagct
     4501 taaaaaaagc acttatgagt gctttttatt actgcttatt attacttaga tagcaacaat
     4561 atttacagca ttcggtcctt tttgaccttg agctacactg aattcaactt gctggccttc
     4621 aaataaggtc ttgaaacctg aactcgcgat ttcgctgaaa tgagcaaaaa cgtctggacc
     4681 tgattcttgt tgaatgaaac caaaaccttt agtttcgtta aaccacttaa cagtaccttt
     4741 aacaacattt gagtttgaca taatatatcc tactaatttt taataatttt ttagtcattt
     4801 tattgactga ttaataactt tgaaaataat aaagaatgag acttaaaatc taaaaaacga
     4861 aggattatga ctaaaactgc gatacttaag gaagatttac cgaaacaaga ctttttttct
     4921 agttaagtta aatatacagt aaaaataaaa ataatcaagt tttttgatat aaatattaat
     4981 ttttcttata aaatccatat ttttttcttt aaaaattaat taaattcaat aagttattta
     5041 ttatattatt taatattttc tttaacataa aatctcatat gcgaattcta gccctgcata
     5101 gggctagata tagcgattag acccaattct ctagcttcag accatcaatc cgttcaaatt
     5161 ctttgatatt gtttgtgact aatacaacat ccaatgccaa agcatgagca gcgatcagca
     5221 tatctaagct gccaatcggt ttacctgtag attgaagatc ttggcgtaat ttggcgtaat
     5281 gccaaattgc ttgttcatcg taaggaagaa tctctaaagg tgacaaaaac ttgtttaacg
     5341 cctgtttatt gcgttcagat ccagacttct ctacaccaaa agccagctcg gatgccgtga
     5401 tacttgaaat tgcgagctgg ccgagttgat attgcttaaa gcgttcaaaa acgtgttgag
     5461 gcttattatt gatgacatag atacaaatat ttgtgtctaa gaggtaaatc atttgaactc
     5521 ctcacggaac tgttccccct gatcagcacg ttctaactgg aatccagatt cgaactcatt
     5581 cactgcttca agcattacat cccaaggatt atcgattggc atcaacaaaa cgccacgtcc
     5641 gaagcttttg atggccactt ccgtgccatt aaagcgaaag gctttaggca agcgaacagc
     5701 ctgactgcga ccagtttgaa atactttcgc aatttccata attacctccc gaaaccaaga
     5761 tatgtatcat tgatatatac catagcatta aatcttgtag agtcaaagag caattaaaat
     5821 cataagacaa taaaaaaacc ccgacatcct gtcggggttt ttcgtatttg gtactagggt
     5881 caactcatgt catccctcgc gtcctgtcgc tgatcttctt atttttatgt ggaacatcct
     5941 gttctcaatg aagtcatgat atgtaatctg gtcgtgtctg cctattggtg attttcttta
     6001 atgcctgtac gccagaacgt acatgcagat atcctcaaat tatcccaaga attgtccgca
     6061 ggaaatgggg ataatttcag aaatgagaac ctttagaaat caggaaaaaa tgaaattttg
     6121 agtatttttt aaccgatttt attggctaaa agtgccagaa gtgattaaaa agcagccgac
     6181 ttgtttccga atcgactttt tgctgatttt ttagtcatga ctaatttttt aaacaattga
     6241 aaaacaatga atttttataa gtgatttcgc ataagcacca ttatgttaaa tgggagaaat
     6301 ttagtgagtg aaaatatcta tttttcttta tacttattat aattttttga tttaaataaa
     6361 attttaatta tgattgatgg aaagacttta cctttacgga cagatgttcc tgaaaatgat
     6421 ttagagactt ttgagaataa acttttaaac ttatgccaag aatatttttt agacctttca
     6481 aagtcagaac ttttacaaac aggtagatcg aaagatattt tggaaaaata taaggaaaac
     6541 aaaagtttaa gtaattttga acaagcctat ataaatgagt accttctaag atctttaact
     6601 caatttttac caagttttct gaaaaggact gcaaataaag ttgaattaga gagattcacc
     6661 gaagaaaaag ttcgtcaaga aaagttgaga caagaagcta ttaaaaaaca aaagttactt
     6721 gaatcagatc ctgtatatat tgcaaagcaa aaagaacaag cattacttaa aaaatatgaa
     6781 attaatgaat atatatcagg tactttaccg aaagaaattt cagaaatttt aagtaaatta
     6841 gagggtggta tcaggctttc ccaagatgaa gctgtttggt taaatactat aggaaagagg
     6901 ttctttagta gtaaggttcg tcataagttt catcgtttag aagctaatca ttatcttcaa
     6961 gaatttgaaa aaaataagaa aaatatttgg aatgctatta atgcgagtag ccaattaaga
     7021 aagtgtcagg caagtcttga agcagaagaa ttgctagaaa aaatttcgat tgctggcata
     7081 aaagacaaaa aactattatc cgcttatttc actaccctcg gcggtgttag acgtgatctt
     7141 cgaaaagttg aagttgcgat tgataatgct tctaaagctc atactttaac acctgataat
     7201 tatcgtccat gtacgttatt aggcgctatt tatatggaga cccgtcagta tacacttgga
     7261 catgaatggt atgaaaaagc tagtgaacgt ggagcaccaa aacaatcaat taatgccgat
     7321 ctaaaaagca ttctttctaa aatggataaa gctcaacgga atgaaatgat tgagcactta
     7381 ttaaaattag attcttatac atactcatgg ctaaagtctt taaaggcagc cgtagtcaat
     7441 aatcctaaaa gtaaacaaga taaagttgag caaccagtaa aagctcaaaa aaaggcatcg
     7501 ccatcacaga gtaaaaagaa tgttcaaaat aaacagccta aaaattctga aaaaagttta
     7561 cagaatcttt tagagcaggg agctaaggca cacaatttaa agcagccagt gaacctatca
     7621 aaatcttcat gtatgaaacc taagaagaag taattttatt attgagtttc gctaaaatta
     7681 acataatacg ccttatacga agctatgaat ccttatcctg taaggcattc atagctcgcc
     7741 acttcatcat taacacctca tcttcctcct caaaggtcga ctcacctgaa gcaatccgct
     7801 caatcatcgc atcgacatac acttgactgg catcaatcac atgctgctca aactcatgct
     7861 ccacgatccc ttgcgttgcc ttaaaagcag ctcgaattcg atctgcatcg actggatttt
     7921 tcatacttgg tcacgctgct ctgattgata acttggatga tctttgagat aggcaataaa
     7981 actgtccaaa cgtttgctat ccaatcgtga ttcatcagga tgatccacat gctgcaatag
     8041 gttctgacct aaaaaaaact gctcaaactg agccttaaaa tactcaggaa tatcagacac
     8101 caaaacatag ctgtcatctg atttcagttg atgccgtggg atctgaatca actcaagtaa
     8161 atgtttcttc cacaatagac tggcccgaag ctgatcattc actggatgat ggggatcatg
     8221 tgcagcagca cgttgatcgt caaataaacc ctcaagaatt tgttttccag tcttcatacg
     8281 acctccaaaa gctttattta aaacctcaca atattcctag aaccatgatg gcgtataacc
     8341 atcatcacga tctagatctt gatttttacg ctgctctaag gcttggttgt actccttttg
     8401 gtagatctgg agcttattaa attgccgaat ctgttgaatg gtttgcttct gtgcctcaac
     8461 ttcaagctga atcattggat cagcactttt caacttccat cggtagatct gtggaacatc
     8521 ctgcaattgc gttccatact tctggttaaa ttcctgatta gacagatgac tcactcgatt
     8581 cggaacaaca atctccatac cctccagaat tacggttttc atcggtgcat gcatttccaa
     8641 agcctgatta attagctgct cagttttttc tcgaaagact ttcacctgat cacggccaaa
     8701 gcccaacgca tcagccgtta atttctgtgc gccaccctta gtgggattct ttggacgata
     8761 ctgcttaaaa aactgctctg gtggtcgttc aatcccatcg gccagactcc gttcagaata
     8821 catcagatgc aaatgcggtt gatcttcacc tgtcagctca ctcggatgat tatgcaccgc
     8881 tgcggtataa gggaactgat actgatcagc aaattcctga ataaaggcct cgaccaattc
     8941 tttacgctgc tgtttcgaca attcttttgg taaagccaca gtcaacgttg aactggttcg
     9001 acctcgagca atctcataac gatccgcact atgccaaaaa gtcgcaggtg catcgacagc
     9061 ccaagcaggc atatttccag aacgcacgaa ctccaattcc tcatcgtcct tttgctcggc
     9121 aaaatgagcc gttcgagtca gataatggaa ggtattcttg gaactggaat ccttcttcac
     9181 attgtgccga atattcatgt aaaacatgcc gaattcacct gagatccaat tcgcaaagga
     9241 ggctctccgc aggagcgagg aggagtgtct ttttttgaca ataatcgctt tagcggttgt
     9301 tgtcaaaaaa agacacggaa acgagataaa ctatttttta tgcatagcag aaaaaatagt
     9361 ttaaacgccc ataatatgac gctaacatgg agtcaatcca cggcgaaatg agatccaaat
     9421 ggcaggacgc aaagcgttag ttctcacagc aaaagaaatc aacgaactcg gctcgcatat
     9481 cctcaatcta ccgttcaaac gccgagttga agaacgttgt ttgcacatgc tcaaaaacaa
     9541 aaagagtctt caggatctca gtgagcaaga ccgacaactg atccagaaat gccgctacga
     9601 acgcaatgcc tacaacaaac gcatgcttca gctgcaactg atccaacaga cagcaccaaa
     9661 caaacagact gcactggaac aaaatatcct caagctccat caaaaacatg atatcgatgc
     9721 ctactttgcc atgcacgatg cactggatga gatcctcaaa acgcaacgcc accagaccgc
     9781 agcgaaaaac ctgaatcaga aaatagaaaa agccctgaat ccagaacaac aaaaggaaaa
     9841 acaaagccag aaacaacaaa aaaaacgaga agaccagatc aagtacttta tcggttcact
     9901 ctatctaggc gtttttgaac gagcaaagtt tcaaatgacc cactccaatc aggacttgga
     9961 caacctgaaa accttatttc gtatggcttt aatcggtaaa accatgcaac aaagcaacaa
    10021 ggatctacag acagtcaccc aagaaattgc taactcaagc caataccaag aaattgaacg
    10081 atttatacga gaagccaaac aagacccaag aaacccattc aacaaaacac cagagcaatg
    10141 aacaaacatc acgtttaaac gctgtatttt cgccatagtg cgatcatttc aaaccgaact
    10201 agagatcagt acccaaaaaa caaaaaagtg tctctaaacg caaatagaag caaatgacat
    10261 agcatctata agcgataaga aggcctcaac caagcgagtg tctacgagcg acatccccaa
    10321 aattcgcgcc tcggccctct gaaaaactgc ttttttaggt tgattaacct aaaaaagtga
    10381 agtttagatg caaaaatttg agcctagacc gagcgaagcg agcataaagc tttatgagcg
    10441 aagcgaattt cgaattgctt ttgctcttgc ctttgctttt tctcaaattc acaacgaaaa
    10501 gaaccaaaaa attgccccca gaatcgagcg aaagcgagat tcaatagagt ttgagcgtag
    10561 cgaaaaccaa gggcaatttt gaccagacta ggcttttaat tatttttaat agtttttaat
    10621 gcttttagac aggctgaaag cctttaatag caaatgtttc aaagcctata aaactaggtt
    10681 tatcgaccca taaaactagg tttatcgacc cataaaacta ggtttatcga cccataaaac
    10741 taggtttatc gacctaatta ataggtcgtt ttacaaccta gttaaaatag tttatggtat
    10801 gacctagtta aatttgattt atattaaaaa a
//
