LOCUS       NZ_AP024804            12817 bp    DNA     circular CON 21-APR-2024
DEFINITION  Acinetobacter baumannii strain OCU Ac18 plasmid pOCUAc18-2, complete
            sequence.
ACCESSION   NZ_AP024804
VERSION     NZ_AP024804.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00330123
            Sequence Read Archive: DRR301936, DRR301937
            Assembly: GCF_019703285.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1
  AUTHORS   Sakiyama,A., Matsumoto,Y., Tsubouchi,T., Suzuki,M., Niki,M.,
            Niki,M., Oinuma,K.I., Kakeya,H. and Kaneko,Y.
  TITLE     Complete Genome Sequence of a Clinical Isolate of Acinetobacter
            baumannii Harboring 11 Plasmids
  JOURNAL   Microbiol Resour Announc 10 (39), e0069521 (2021)
   PUBMED   34591663
  REMARK    DOI:10.1128/MRA.00695-21
REFERENCE   2  (bases 1 to 12817)
  AUTHORS   Tsubouchi,T., Sakiyama,A., Matsumoto,Y., Suzuki,M. and Kaneko,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUL-2021) Contact:Taishi Tsubouchi Osaka City
            University, Bacteriology; Abeno, Asahimachi 1-4-3, Osaka, Osaka
            545-0051, Japan URL :http://www.med.osaka-cu.ac.jp/bacteriology
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Illumina Novaseq; ONT Flongle
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019703285.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 04:56:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,961
            CDSs (total)                      :: 3,865
            Genes (coding)                    :: 3,655
            CDSs (with protein)               :: 3,655
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 210
            CDSs (without protein)            :: 210
            Pseudo Genes (ambiguous residues) :: 0 of 210
            Pseudo Genes (frameshifted)       :: 58 of 210
            Pseudo Genes (incomplete)         :: 134 of 210
            Pseudo Genes (internal stop)      :: 38 of 210
            Pseudo Genes (multiple problems)  :: 17 of 210
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP024804.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12817
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="OCU Ac18"
                     /isolation_source="venous blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="pOCUAc18-2"
                     /geo_loc_name="Japan"
                     /lat_lon="34.68639 N 135.52 E"
                     /collection_date="2016-07-16"
     gene            49..930
                     /gene="repM"
                     /locus_tag="OCUAc18_RS19395"
                     /old_locus_tag="OCUAc18_38010"
     CDS             49..930
                     /gene="repM"
                     /locus_tag="OCUAc18_RS19395"
                     /old_locus_tag="OCUAc18_38010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004734502.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_005804946.1"
                     /translation="MNKNHVVKSNQVIEASYQLSAVEQRIVLAAISRIPKNQPITDDEL
                     YPVSVDELKQLGVHEKTAYRDLKEGINRLYERSINLSIDDKSIKMRWVQEIQFLDSQSV
                     IGIRFSKPILPFISNLSREFTKYALSDIAGINSGYGIRIYELLVQYRQIGKREISVESL
                     RTMLELGKKYPLFADFKKRVIDTAIDQINEYSPLKVTYEQKKTGRKVTHIIFSFKEKSK
                     SITHTSKDFYQLSDAQINLFGNQLSRLHELSHLASEGESYEILAAKIKDMLRNPHQQQQ
                     FLPHLKNLGFKA"
                     /name="repM"
     gene            994..1560
                     /locus_tag="OCUAc18_RS19400"
                     /old_locus_tag="OCUAc18_38020"
     CDS             994..1560
                     /locus_tag="OCUAc18_RS19400"
                     /old_locus_tag="OCUAc18_38020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_000858429.1"
                     /translation="MKTSTKYSVSDAATLYKKSRATLYKDIKNGVLSRDHDGSIDFSEL
                     LRVYGEPFSGKHSKHTDTSPTQTINTPEYTDNTVIELKNQIDFLKKQLEKAEDREAKAN
                     SRIDTLLTLIEMKPTQVHPTPTSDQSSVQSSPSQVEPTLNVDNDKHDGLTTPEKTENKR
                     IPVPEPVEPEQPKRGWLSRFFLPNG"
                     /name="plasmid replication DNA-binding protein"
     gene            1818..3128
                     /locus_tag="OCUAc18_RS19405"
                     /old_locus_tag="OCUAc18_38030"
     CDS             1818..3128
                     /locus_tag="OCUAc18_RS19405"
                     /old_locus_tag="OCUAc18_38030"
                     /inference="COORDINATES: protein motif:HMM:NF024573.4"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_221255520.1"
                     /translation="MLSKIRILNYKSLNDIYFDPTHVNLFLGENGAGKSNILEALAMYS
                     AAKSNMLSNEFLISRGIRPIDPLTTLSQFPLVEKDKSDNEERSKNFAIFTQDNLLPIGV
                     AICHDEEDPYRGLKAKIFTARFDENDDGEKSYLIDENIEESHSKVMNDLFKSFSEINKL
                     FPQVEDINEIYKEKFIKELKKKDKNKISKLLEIQNKMHYLSENLKLIDGFRSKKSSKDN
                     FVIFNPEINTLMGENTQSQIQPLGIHGEGLFTLLKVMAKKEPENFKDVIDTASIFNWLE
                     KIELVEDLNEQKIVLKDRFMDGYISPKSANEGFLFSLFYACLFCSKQTPSVFAIESIDK
                     SLNPRLCQVLVKKLVEKAKKYNKQVFLTSHNAAVLDGMDLLDKDQSIFIIERATSGETK
                     IRKLTEEHLPKPKRNGEKLNLSEAFLRGMLGGLPSNF"
                     /name="AAA family ATPase"
     gene            3143..3814
                     /locus_tag="OCUAc18_RS19410"
                     /old_locus_tag="OCUAc18_38040"
     CDS             3143..3814
                     /locus_tag="OCUAc18_RS19410"
                     /old_locus_tag="OCUAc18_38040"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_221255521.1"
                     /translation="MRFSIIGEGSTDFRVLKTILESFFVDSEINSLVPVLDASTLKTAK
                     DEHGSWTKIPEYLKSNFLEDALVNTDYLVIQIDTDVCEEPGFDVGPKVLADSNQEDFYN
                     LVQAKLIEWIDSFEPDTYNYYQEKFIFAIAIHSLECWLIAYYCKSSELKIKRIKNGFNH
                     LQRALERDKKIHLAEPKDPAVYEELAIPFRKRKHHAQARTYSYSLNRFMCQLEEIESKM
                     V"
                     /name="hypothetical protein"
     gene            complement(3885..4124)
                     /locus_tag="OCUAc18_RS19415"
                     /old_locus_tag="OCUAc18_38050"
     CDS             complement(3885..4124)
                     /locus_tag="OCUAc18_RS19415"
                     /old_locus_tag="OCUAc18_38050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008486742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_017482479.1"
                     /translation="MSEQQIYERIRLAMNEAPRNRQTAELHLQMIKYADDLKNITSKEF
                     CEGVGLPLSYGTEFSKMRNITERLKAAGLKVNMI"
                     /name="hypothetical protein"
     gene            4458..5417
                     /locus_tag="OCUAc18_RS19420"
                     /old_locus_tag="OCUAc18_38060"
     CDS             4458..5417
                     /locus_tag="OCUAc18_RS19420"
                     /old_locus_tag="OCUAc18_38060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000941154.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="WP_004706950.1"
                     /translation="MLQNSKRIGFLLLDNFSMIAFSNMLEVLRMANYVIEDNLYQWNVT
                     GLDGHHSCASNGLQIQHTCVLSHLLNMDIVFICGGFQLYKNMTPRLKNFLYQLDQKKIA
                     LGGLCTGAYVLAKAGLLNGFRASMHWENTLAAQEEFPLVQFTTHIFTIDVNRFTCSGGL
                     AAIDLGLQLVEMHYPNITKKICEQFIVHQIRDGNEIQHQPISKHSKKLDLPIQEVLRLM
                     ENNINEPLKISEIAQHVKLQSRTLERLFKIHLQISPKEYYLKLRLTHAQNLLKQSTLSI
                     SNIAFACGFCSASSFTKAYKAFFNYSPKFERNVLDFAN"
                     /name="GlxA family transcriptional regulator"
     gene            5533..6771
                     /locus_tag="OCUAc18_RS19425"
                     /old_locus_tag="OCUAc18_38070"
     CDS             5533..6771
                     /locus_tag="OCUAc18_RS19425"
                     /old_locus_tag="OCUAc18_38070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001094923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="WP_004706948.1"
                     /translation="MNTLVNNFAVSELPSLLTIENGEKVSATFSLSEYQNRQSKLRQLM
                     EELEIDHVLFSSIHNINYYADFIYCSFGRFYGLVVSPEKVVTISANIDAGQPWRRTVGD
                     YNVVYTDWQRDNYFKAVAQEIPNRGRVGIEFDHLPLERLNKLKTVLPNVQFVDISAACM
                     KLRMIKSAEEIAHITQGAAVCDIGGFAVTAAIREGVPEHEVALASTQAMVREIAKRFPN
                     SELMDTWTWFQSGINTDGAHNPVTTRQVQKGDILSLNCFSMIAGYYTALERTQFFDHCD
                     DASLRIWEANVKVHEAGLKLIRPGARCSDIAKELNEIFYAEGLLQYRTFGYGHSFGVLS
                     HYYGREAGLELREDIDTVIEPGMVISMEPMVMIPQGMAGAGGYREHDILVVTETGNENI
                     TKFPYGPEHNIIK"
                     /name="M24 family metallopeptidase"
     gene            6826..8118
                     /locus_tag="OCUAc18_RS19430"
                     /old_locus_tag="OCUAc18_38080"
     CDS             6826..8118
                     /locus_tag="OCUAc18_RS19430"
                     /old_locus_tag="OCUAc18_38080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006581599.1"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="WP_004706945.1"
                     /translation="MTKDNQVEQKKTLKRVASASFIGNFVEWFDYAAYGFLATVIAVVF
                     FPQSDPLTALMAAYAIFAISFILRPLGGIFWGHVGDKFGRKNALSWSIILMTLATVCIA
                     LLPSYQSIGIFAPILLLVFRMIQGFSASGEYAGAANFLAEYAPKKKRGLYTSLVPASTA
                     AGLLLGSVMAAAMFAFMSEAFLHEYGWRIPFLLAAPLGLIGYYIRVKLEDTPEFLEHQK
                     TGQKENFPIKALFNQYRPALFKAFAVASLNATAFYLIFNYMPNYLATELGVNKTQAFIS
                     GSISLLFYIAVVFAMGKYSDRIGRKKMLLWASLSFIALTIPLFYLLSTASFVEMVMIQL
                     VFCTLLAMNDGSLPAYLTEQFPIQVRYTGFAFSFNTANALLGGTVPLVATWLIQQTGNT
                     LAPSVLLIVVAVFASMALLQRKSHRLVSAKA"
                     /name="MFS transporter"
     gene            8131..9036
                     /gene="yddG"
                     /locus_tag="OCUAc18_RS19435"
                     /old_locus_tag="OCUAc18_38090"
     CDS             8131..9036
                     /gene="yddG"
                     /locus_tag="OCUAc18_RS19435"
                     /old_locus_tag="OCUAc18_38090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004706944.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid DMT transporter YddG"
                     /protein_id="WP_004706944.1"
                     /translation="MLLNALPALNRNISTLIGLTAVIFWSTNVGLIRSVSESFGAVAGA
                     ALIYSFASMILVFTIGMPKFSTIPKSYLWWGSLLFVAYELCFALSIGYAQNSRQAIEVG
                     MINYLWPTFTLVFAIIFNNVKANLLIIPGCILALVGICWVLGGDTGFNPFQIWNNLKVN
                     PLSYGLAFAAAILWAAYCSVTVKTSQGKNLVTIFFALVAVVLWIKYFFMSGSAINFTYD
                     NTVTLIMAAGALGLGYGAWNIGIISGNMTLMAGASYFTPVLSAFFAALLLSTSLSFIFW
                     QGVAMVTTGAVLCWLATRKQ"
                     /name="yddG"
     gene            complement(9301..9525)
                     /locus_tag="OCUAc18_RS19440"
                     /old_locus_tag="OCUAc18_38100"
     CDS             complement(9301..9525)
                     /locus_tag="OCUAc18_RS19440"
                     /old_locus_tag="OCUAc18_38100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004676219.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_004676219.1"
                     /translation="MPYEEFQRLMGKAGLSIKEFATLLDMNPNSITNYKKIGKVPTHIA
                     VLVYLLSSMKDEGVDFYPIFEKIKSYSKD"
                     /name="helix-turn-helix transcriptional regulator"
     gene            9675..9920
                     /locus_tag="OCUAc18_RS19445"
                     /old_locus_tag="OCUAc18_38110"
     CDS             9675..9920
                     /locus_tag="OCUAc18_RS19445"
                     /old_locus_tag="OCUAc18_38110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005249014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004282220.1"
                     /translation="MYSYPDSNTEKKIALMIINDFFIQKAHELWIFLQLDQCFNDYEAT
                     LIWTRRYLEKHPECEYSDIQKAFVSCFPEHFFSFDC"
                     /name="hypothetical protein"
     gene            complement(10212..10973)
                     /locus_tag="OCUAc18_RS19450"
                     /old_locus_tag="OCUAc18_38120"
     CDS             complement(10212..10973)
                     /locus_tag="OCUAc18_RS19450"
                     /old_locus_tag="OCUAc18_38120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017393773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000425105.1"
                     /translation="MEMEKAKKRGRPAQLLQIAELHAFVEFLEQQEQLSDLQSQVLKAL
                     NSVDCNFEGLTQTDQVLVKEALKPYREHLKLKLLFEELNNLPLKTEYEQKFLDLYELFQ
                     KNALDQMELNILKTLATRYLNFKAQKLEYSDLELYLSQLQKKDAGKKRKAENQRKFELG
                     GAVLVAFKKLNIDISNDTPQQITNRIVNTTKFHNEVRKSLIFKDVKTYENEYFKANKLF
                     IQVLEGLHTWQKGGELLSVIEIKKALEKGEE"
                     /name="hypothetical protein"
     gene            11149..12318
                     /locus_tag="OCUAc18_RS19455"
                     /old_locus_tag="OCUAc18_38130"
     CDS             11149..12318
                     /locus_tag="OCUAc18_RS19455"
                     /old_locus_tag="OCUAc18_38130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002011489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_004698297.1"
                     /translation="MASYHFSVKSKNKGYATGHYLYISRLMQYEGIRKTSNETIEHIEP
                     GRCMPAFVKDPIDFWQAADTYERANAKAYMEYEIALPNEFTSEQRKTLIETFLDKHIVP
                     QQYPHSYAIHNVKSRISGEDQPHCHLMFSLKADDGIERSAEQYFKRYNPQDPAKGGAKK
                     IQLQDGHEDYSTFLIYIRKQWENHLNDALAQHCPTVTYTLDGQDITIKNQVSADSYEKY
                     NEIHSTLYLPEPKLGVGQQNATAEYLAQIQKIREHNQQERELEERQKQLDQQHEYFYSA
                     ENNPFSSLEVMYYLNYLIKNATNKDKLKNDLNEIIKNQGPILKQEAWSIYEMNEKLEDS
                     RRNSTPCFEFKVHSLPEYVLPIQNTPPKRTQSEPVQPKADDYDYGGPGF"
                     /name="MobA/MobL family protein"
     Typing          50..928
                     /name="rep_cluster_1364"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.81"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          11228..12315
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.725"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgacaagta tatatttgtc atatattaga aaaatcaatg gttttgtttt gaataaaaat
       61 cacgttgtaa aatcaaatca agtaatagaa gcatcgtatc aattaagtgc cgtagagcaa
      121 cgaattgtct tagctgccat ttcacggatt cccaaaaatc agcctattac agatgatgag
      181 ttataccctg ttagcgtcga tgaattaaaa caattgggcg tacatgagaa aactgcatac
      241 agagatttaa aggaaggtat taatagactc tatgagaggt ctattaacct cagtattgat
      301 gataaatcta taaaaatgag atgggtgcaa gaaatccaat ttttagatag tcaaagtgtc
      361 attggaatcc gtttttcaaa acccattctg ccatttattt ccaatctaag tagagaattc
      421 accaaatatg ccttatcaga cattgctggg atcaatagtg gatacggtat tcgtatttat
      481 gagctactgg ttcaatatcg gcaaataggt aagcgtgaaa tttctgttga gagtttacgg
      541 acaatgttag aactcggtaa aaagtaccca ttattcgctg atttcaagaa acgagtaatt
      601 gacactgcta tagaccaaat caatgaatat agccctttaa aagtcactta tgagcagaaa
      661 aaaacagggc gtaaagtcac tcatattatc ttttctttta aagaaaaatc taaatccata
      721 acacatacct cgaaagattt ttatcagcta tctgatgctc aaattaatct attcgggaat
      781 caattatcac gtttacatga gctttcccat ttagcctcag aaggtgaaag ttatgagatt
      841 ttagctgcta aaattaagga tatgcttaga aaccctcacc aacaacagca gtttttgcca
      901 cacttaaaga acttagggtt taaagcttga gtgtattgaa gaatacactt attgtattct
      961 tcaatacgta tcctaaatac acagcaatac actatgaaaa caagtacaaa atacagtgtt
     1021 agtgatgcag ccacactgta taagaaatct cgtgctacac tttataaaga tataaaaaat
     1081 ggggtgttaa gtcgtgatca tgatggctct atcgacttct ctgagttact ccgcgtttat
     1141 ggtgaacctt ttagtggaaa acactcaaaa cacactgata cgtctccaac acaaactata
     1201 aatacacctg aatacacaga caatacagtt attgaactaa agaatcagat cgattttcta
     1261 aagaaacagt tagaaaaagc agaagatcgc gaagctaaag caaattctcg tattgataca
     1321 ttacttaccc taattgaaat gaagccaact caggttcatc ccacaccaac ttcagatcaa
     1381 tcttcagtgc aatctagtcc atctcaagta gaacctacat tgaatgtaga taacgacaaa
     1441 catgacggat tgactactcc ggaaaaaacg gaaaataagc gtattccggt gccggagccg
     1501 gttgaaccgg agcagccgaa aagagggtgg ttaagccggt ttttcttgcc gaatggctaa
     1561 gggtactatt caactgagct aatcaaagtt gtaaggattt gattaaagaa tagccatatt
     1621 acgtgaaaga tcacaaaaaa aagcccgtac agggatgttc gggctataaa atagaaacta
     1681 cagcattgat ttctatagat aattataacg catttcgtat aaggcgtatt atgttaattt
     1741 tagatactct taaaagttcc tatctagctc tgtgttcatg ctaatatcat tgaaacttat
     1801 ctttatgact atctgaaatg ctttctaaga ttcgaattct taactataaa tcactaaatg
     1861 atatttactt tgatccaact catgtaaatc tatttttagg tgagaatggt gctggaaaat
     1921 ctaatatttt agaagcttta gcaatgtata gtgctgcaaa atcgaatatg ttgagtaatg
     1981 aattcctgat atcaagagga atacgcccaa tagatccatt aacaacactt agccaattcc
     2041 ctttggtaga aaaagataag agtgataacg aagaacgatc taaaaatttt gcaattttta
     2101 cccaagataa tcttctacct atcggggtag ctatctgcca cgatgaagaa gatccatatc
     2161 gtggtttaaa agctaaaata tttacagcta gattcgatga aaatgatgat ggtgaaaaaa
     2221 gttatttaat agatgagaat attgaagaaa gccatagcaa ggtaatgaat gatttattca
     2281 aaagcttttc tgaaatcaat aaactgttcc ctcaagtaga ggatattaac gagatttata
     2341 aagaaaaatt tattaaagaa ttaaaaaaga aagataaaaa taaaatttct aagttattag
     2401 aaattcaaaa taaaatgcat tatttaagtg aaaacctaaa acttatagat ggtttcagat
     2461 ccaagaagtc atctaaagat aattttgtta ttttcaaccc tgaaataaac acactaatgg
     2521 gcgagaacac tcaatcacaa attcagccat taggaataca tggggaagga ttattcaccc
     2581 tgttaaaagt catggcaaaa aaagaacctg aaaactttaa agatgtaatt gatacagctt
     2641 caattttcaa ttggttagaa aagatagaac tggtcgagga tctaaatgaa caaaaaattg
     2701 ttctcaaaga tcgcttcatg gatggatata tttcccctaa aagtgctaat gaaggctttc
     2761 tatttagctt attctacgcc tgtctctttt gctcaaaaca aactccatct gtatttgcaa
     2821 tcgagagtat tgataaatca cttaacccac gtttatgcca agtcttagta aaaaaattag
     2881 ttgaaaaggc gaagaagtat aataaacaag tcttcttaac cagtcataat gcagcagtgc
     2941 tagatggcat ggatttactt gataaagatc aatctatttt tattattgaa agagctacca
     3001 gtggtgaaac taagattaga aaattaacag aagaacatct acctaagcct aaaagaaatg
     3061 gtgaaaaatt aaatctttca gaagcttttt taagaggcat gctaggcgga ttaccttcaa
     3121 atttctaatt ggtataaaaa ttatgcgttt ttctattatt ggtgaaggta gcacagattt
     3181 tcgagtattg aagactattc tagaatcatt ttttgtagat tctgaaatta actctttagt
     3241 gcctgtctta gatgcctcga cattaaaaac agcgaaagat gaacatggta gttggactaa
     3301 aattccagag tatttaaaat caaacttcct tgaagatgca ttagtgaata cagactattt
     3361 agttattcaa attgacactg atgtttgtga agagcctggt tttgacgtag gccctaaagt
     3421 tctagccgat agtaatcaag aagattttta taatttagta caagctaaat tgatagaatg
     3481 gattgatagt tttgaacctg atacctataa ctattatcag gaaaaattta tatttgcgat
     3541 tgcgattcac tctttagaat gttggttaat cgcatattat tgtaaaagtt cagaactaaa
     3601 aattaaaaga attaaaaatg gttttaatca tctgcaaagg gctttagaaa gagataaaaa
     3661 aatccactta gcagagccaa aagatcctgc tgtatatgaa gagttggcaa ttccttttag
     3721 aaaaaggaag catcatgctc aagcaagaac ctattcatat agtttgaata gattcatgtg
     3781 ccaattggaa gaaatagaaa gcaaaatggt ataaacattt aacataaaat ctcttatgcg
     3841 aaatttaaaa gggcttagtc atgactaagc ccttttatta gcttttaaat catatttacc
     3901 ttaaggcctg ctgcttttaa gcgttcagta atgtttctca ttttactaaa ttcagtaccg
     3961 taactaagag gtagtccgac accttcacag aactctttag atgttatatt ttttaaatca
     4021 tctgcatatt taatcatttg caaatgtagc tctgccgttt gtctatttct aggagcttca
     4081 ttcatagcca atcgaatacg ctcatatatc tgttgctcac tcatatctaa tttatccaat
     4141 taatcaaatt tatataaaaa atataattta tataaatttg attgtcaatt ttttgttcaa
     4201 tcttattggc gaaagatgac aaatatgatt aaaaaaaaag ccgacatgtg cctaagtcgg
     4261 cttttcacct attttttagt tatgactaat tttttaatcc attgaaaaac aaaaactttt
     4321 tacaggtgat ttcggataac ggtcattatg ttaaatggat agttatttgg tggaaaattt
     4381 aaatttttaa agaatcgaaa ttgtggtctt aaacttgctt actaaaaata tgaaagaaat
     4441 gaagtaaatt aattcttatg ttgcaaaact ctaagcgtat tggttttcta ttactcgata
     4501 acttttctat gattgccttc tcaaacatgc ttgaagtatt acgtatggct aactatgtca
     4561 tagaagataa tttataccaa tggaacgtaa cgggtttaga tggacatcat agctgtgcaa
     4621 gtaatggctt acaaatacaa cacacctgtg ttttatcaca tttactcaat atggacattg
     4681 tctttatttg tggtggcttt caattatata aaaatatgac gccacgatta aagaattttt
     4741 tatatcaact tgatcagaaa aaaatcgctc taggtggttt atgtacgggt gcatatgtat
     4801 tggcaaaagc gggtttgctc aatggctttc gtgccagtat gcattgggaa aatactttag
     4861 ccgcacaaga agaatttccc ttagtacaat ttaccactca tatttttact attgatgtga
     4921 atcgtttcac ctgttctggt gggcttgccg caattgattt aggcctacag cttgttgaaa
     4981 tgcactatcc taatattact aaaaaaattt gtgagcaatt tattgtccat caaatacgtg
     5041 atggcaatga aattcaacat cagcctattt ctaaacacag taaaaagctt gatttgccga
     5101 ttcaagaggt gttgcgctta atggaaaata atattaatga acctttaaag atttctgaaa
     5161 ttgcacagca tgtaaaactg caatcacgta ccttagaacg cttatttaaa atacatctgc
     5221 aaatatctcc gaaagaatat tacttaaaac taaggcttac tcatgcacaa aatttactga
     5281 aacagtccac tttgagcatt tcaaatattg catttgcatg tggtttttgt agtgcttcta
     5341 gttttactaa agcctataaa gcttttttta actattcacc caaatttgaa cggaatgttc
     5401 tagattttgc taattagtat tttttaatat tgtcagtttt tgacaacttt ctgtcgtttt
     5461 tggcgaaaac acctattcat attctgcata agcttagttg attggttaca tcacattgat
     5521 ctaggcacat atatgaatac tttagtaaat aattttgctg tttcagagct accatcatta
     5581 ttaaccattg aaaatggtga aaaagtttct gcgacatttt cattatctga gtatcaaaat
     5641 agacaaagca agctacgcca actaatggaa gagctagaaa ttgatcatgt tctatttagt
     5701 tcaatacata acattaacta ttatgcggac tttatttatt gttcatttgg tcgcttctac
     5761 ggtctggtgg ttagcccaga aaaagtggtg acaatttcag caaatattga tgctggtcaa
     5821 ccttggcgtc gtacggttgg tgactataac gtggtttata ccgattggca gcgtgataac
     5881 tattttaaag cggttgcaca agaaattccg aatagaggtc gtgtaggaat tgagtttgat
     5941 cacttacctt tagagcgttt aaacaagtta aaaacagttt taccgaatgt gcagtttgtc
     6001 gatattagtg ccgcatgcat gaagctacgc atgattaaat ctgctgaaga aattgcacat
     6061 atcacccaag gtgctgcagt gtgtgatatt ggtggttttg cagtgactgc agcgattcgt
     6121 gagggtgtac cagagcatga agtagcttta gcctctaccc aagccatggt acgtgaaatt
     6181 gccaaacggt tcccaaactc tgaactaatg gatacgtgga cctggttcca atcaggcatt
     6241 aataccgatg gggcacataa tccggttacg acacgccaag tgcaaaaagg tgacatctta
     6301 agtttaaact gcttttctat gatcgcaggc tattacacag ccttagagcg tacacaattc
     6361 tttgatcact gtgatgatgc ttcactacgt atttgggaag cgaatgttaa ggtgcatgaa
     6421 gcaggcttaa aactcattcg cccgggtgca cgttgcagtg atattgcgaa agaattaaat
     6481 gaaattttct atgcagaagg cttattacaa tatcgtacct ttggttatgg gcattctttt
     6541 ggcgtcttat cgcattatta cggtcgtgaa gcaggtttgg aattacgtga agatattgat
     6601 acggtgattg aaccaggcat ggtgatttct atggaaccta tggtcatgat tccacaaggc
     6661 atggctggtg ctggtggcta ccgtgaacat gatatcttgg tggtaactga aacaggcaat
     6721 gaaaatatca ctaagttccc atatggccca gaacataaca tcatcaaata atcaattgcg
     6781 tagacacttc ttttagaggt gtctatttga tttaagggga caaaaatgac aaaagacaat
     6841 caggtagagc aaaaaaaaac cttaaaacgt gtcgcaagtg caagcttcat tggcaatttt
     6901 gtagaatggt ttgattacgc tgcatatggt tttttggcaa cagtcattgc agtggttttt
     6961 tttccgcaaa gtgatccttt aactgcatta atggctgcct atgcaatctt tgcaatttca
     7021 tttattttac gtccattagg cggtattttt tggggccacg ttggggacaa gtttggtcga
     7081 aaaaatgcac tatcttggtc gattatctta atgaccctag cgacagtatg tatagcacta
     7141 ttaccaagct atcaaagtat cggtattttt gcaccgatcc tgctcttagt attccggatg
     7201 atacaaggtt tttcagcttc aggtgaatat gcaggtgctg caaattttct tgcagaatat
     7261 gcccctaaga aaaaaagagg actttatacc agtttagttc ccgcaagtac tgcagcgggt
     7321 ttactactcg gatctgtaat ggcagcagcg atgttcgctt ttatgtcaga ggcatttcta
     7381 cacgaatatg gttggagaat tccattcttg ttggccgcac cattgggcct aattggatat
     7441 tatatccgtg taaagctaga agacacgcca gaatttttag aacaccaaaa aactggccag
     7501 aaagaaaatt ttccaatcaa agctttattt aatcaatatc gaccagcact ttttaaagca
     7561 tttgctgttg catctttaaa tgcgactgcg ttctatctta tttttaatta catgccaaac
     7621 tatttggcaa cagaattagg cgtaaataaa acccaagcat ttatctcagg atcaatttca
     7681 ctgctatttt atatcgctgt agtttttgcg atgggaaaat attcagaccg aattggacgc
     7741 aagaaaatgt tgctttgggc aagtctaagc tttatcgcac ttacgatccc tttattctac
     7801 ttactttcaa cagccagttt tgttgaaatg gtgatgatcc aactggtgtt ttgcacattg
     7861 ttagcaatga acgatggtag tttacctgca tatttaacag aacaattccc tatacaagtg
     7921 cgctataccg gttttgcctt tagttttaat actgctaatg cactgttagg tggaacagtt
     7981 cctttggttg caacctggtt aattcaacaa acaggtaata ccttagcacc tagtgtctta
     8041 ttaattgtag ttgcagtttt tgcaagtatg gctttattac aaaggaaatc acatcgttta
     8101 gtcagtgcta aagcttaaaa ggaattagtt atgcttttaa atgctctacc tgcactcaat
     8161 agaaatatat cgactttaat tggtcttaca gctgtcatat tttggagtac aaatgtaggc
     8221 ttaattcgta gtgtcagtga gagctttgga gctgtagccg gtgctgctct aatttacagt
     8281 ttcgcatcca tgattttagt tttcacgatt ggtatgccta agttttctac aatcccaaaa
     8341 tcctatttat ggtggggaag ccttttattt gttgcttatg aattatgttt tgctctttct
     8401 attggctatg cacaaaacag tagacaagca atcgaagttg gtatgattaa ttatctatgg
     8461 ccaacattta ccctagtatt tgcaattatt tttaacaacg ttaaggccaa tcttttaatt
     8521 attcccggct gcatattggc tttggttgga atttgttggg tgctaggggg ggatactgga
     8581 tttaatccct tccagatatg gaacaatttg aaggttaatc ctcttagtta tggtttagct
     8641 tttgctgctg ctattttgtg ggctgcatat tgttctgtga cagtaaaaac atctcagggt
     8701 aaaaatctag tcactatatt ttttgccctg gttgcagttg tactatggat aaaatacttt
     8761 ttcatgagtg ggtcagctat taattttact tatgacaata cagtcactct gattatggcg
     8821 gctggtgctt taggtttggg atatggtgct tggaatattg gaattatttc agggaacatg
     8881 acattaatgg ctggcgcttc ttattttact cctgtgttat ctgctttttt tgcggcatta
     8941 cttctaagta cttccttatc tttcatcttt tggcaaggtg ttgcaatggt gacaacagga
     9001 gcagtcttgt gttggttagc gactcgaaag caataaatta tttagaggtt ttaaaattaa
     9061 cataatacgc cttatgcgaa attccaattg taactccaaa atagaaatgt ccggtttacc
     9121 gattgctcga cctaagatct cagaacataa gaatttgatg atcaccctgg ttctcaaaac
     9181 tcgatacttt attgaaaagt tctatcgagg cttataagtc cctgaaacaa aaaaatgcct
     9241 gaacttcagg cattttttac gtcatcacta acagcttaca acaatcaaca aacgagagtt
     9301 ttagtctttt gaatacgact taattttttc aaaaataggg taaaagtcta caccctcatc
     9361 tttcatgcta gaaagtaagt aaaccagaac agcgatatga gtaggtactt ttccgatctt
     9421 tttatagtta gttatggagt ttggattcat gtccaataag gttgcaaatt ccttaattga
     9481 aagacctgct ttccccataa gtctttgaaa ttcttcgtat ggcatcgtga taaatacaat
     9541 ctgtttgaat tagaagtata acacagacta taaaacttaa aaaaacacat aaaaaatgtg
     9601 acaaacacat tttttatgtg ttataaatag cttatcaact aacctattta tccttactat
     9661 cggagaaatg taatatgtac tcatacccgg attcaaacac tgaaaagaaa attgctctca
     9721 tgatcatcaa tgactttttc attcaaaaag cccatgaact gtggatcttt ttacaactcg
     9781 atcagtgctt taatgattat gaagccacac tgatttggac tcgacgttat ttagagaagc
     9841 atcctgagtg tgagtacagc gatattcaaa aagcctttgt ttcttgtttc ccagagcact
     9901 tcttcagctt tgactgttaa tttatgagag gcacggtaaa aaatcgcaat gggtaagtaa
     9961 atcccaaata gaaagagcca gttcatacaa ctggctcttt ctattgtcac gtataaatct
    10021 aagactttct ttgagcatac taaaattgat gttccacggc ttctaaaaca ttgattttag
    10081 gttcaggatt ggggtcgcca tgacgttact ttagggtgta ctctaaattt acataatgtc
    10141 tctgtatacg aaattcgatt gtaagcccaa aatagaaatg tccaacttct ctaaaatccc
    10201 tgctaaataa actactcttc gcctttttct aaggcctttt taatttcaat tactgacaat
    10261 aattctccgc ccttttgcca ggtatgtaat ccttctaaca cctgaataaa aagtttattt
    10321 gctttaaaat attcattttc atatgtctta acatctttaa aaattaaaga tttacgtact
    10381 tcattatgaa actttgtcgt attcacaatc cgattggtaa tctgttgagg agtatcattg
    10441 ctaatatcaa tattgagttt cttaaatgcg acaagaacag caccacctaa ttcaaattta
    10501 cgttgatttt cagctttgcg ttttttccca gcatccttct tctgtagctg actcaaatac
    10561 aattctaaat cactgtattc aagcttttgc gctttaaaat tcagataccg tgtcgccaag
    10621 gtttttaaaa tatttagttc catctgatct agtgcattct tttgaaaaag ttcatacaga
    10681 tctaaaaact tctgttcata ctcggtttta agaggcagat tattcaactc ttcaaacagc
    10741 aatttcaact tcagatgttc acgataaggt tttaaagcct ctttgaccag aacctgatct
    10801 gtctgagtta gcccctcaaa attacaatca acactattta aagcctttaa cacttgagat
    10861 tgaagatcag aaagctgttc ttgctgttct aaaaactcta caaatgcatg taactcagca
    10921 atctgtaaca actgagcagg tcgaccacgc ttttttgctt tttccatttc catcatctgc
    10981 cgccaaattg acagtttctc ctcatcggag aaactagtac gcaatggggc gcttccccct
    11041 tgaccccctt caaccggatt tttcagccat acaggcattt caaatccggg cggttttcat
    11101 tccgtttcac tgcataaaaa ccgaacccac aacccagcag aggtgaacat ggccagctac
    11161 cacttcagcg tcaaaagcaa aaataagggc tatgcgaccg gccattatct ctacatttcc
    11221 cggctgatgc agtatgaagg catccgcaaa accagcaatg agaccataga acatattgaa
    11281 cctggtcgat gtatgcctgc ctttgtaaaa gatcccattg atttttggca ggcagcagat
    11341 acgtatgaac gagccaatgc caaggcctac atggagtatg aaattgccct accgaatgag
    11401 ttcacgtcag aacagcgtaa aacgctcata gagacgtttt tagacaaaca tatagttcca
    11461 cagcaatatc cgcactcata cgccatacac aacgtaaaat cacgtatcag tggcgaagat
    11521 cagcctcatt gtcatctgat gttctcgcta aaagcggatg acggcattga acgcagcgca
    11581 gaacagtact tcaaacgcta taaccctcaa gatccagcca aaggcggtgc caaaaaaatc
    11641 caacttcaag atggccatga agactattcc acttttttga tctatatccg aaaacaatgg
    11701 gaaaatcatt tgaatgatgc ccttgcacag cactgcccga ccgtcaccta cacactggat
    11761 ggccaagaca tcaccatcaa aaatcaggtt tcagcagaca gctatgaaaa atataacgag
    11821 atccacagca cgctatacct tccagagcca aaactaggcg ttggacaaca aaatgcgaca
    11881 gcggaatatc tcgcccaaat ccaaaaaatc agagaacaca accagcaaga acgagaactc
    11941 gaagaacgcc aaaagcaatt agatcaacaa catgaatatt tttatagtgc tgaaaacaac
    12001 ccattttcat cattggaagt catgtattac ctgaattact taatcaagaa tgcaacaaat
    12061 aaggacaaac taaaaaatga tttgaatgaa attatcaaaa accaaggccc tatacttaaa
    12121 caagaagctt ggtccatcta cgaaatgaat gaaaaactag aagactcaag acgtaacagt
    12181 accccatgtt ttgagttcaa ggtgcattca cttcctgaat acgtattacc gatacaaaat
    12241 acgcctccca aacgcactca atcagaacca gtacaaccta aagctgatga ttatgattac
    12301 ggtggccctg gtttttaagc tcagttggtt ttatccaaag tttacagagc gagtgtctac
    12361 gagcgaacag acacaattaa aatttttgcc atgctttttc cctgattaca aactcccctt
    12421 tgctgctgag actgagatac agagcatccc ggagggtgcg aacagattta atttgcatct
    12481 aggtcgagcg aagcgagtat aaaaaactta tgagcgaagc gaattccgag ttgcttttgc
    12541 ttttttgaag ttatcacgag attaacccaa aaattgcccc ttcgaactga gcgtaagcga
    12601 agttcaatag agtttgagcg tagcgaaaac atagggcaat ttttcattct ttccttttta
    12661 atatatttta aatcttttaa atcttttcag tcgtctctgt gccttatata gcaaaggttt
    12721 cagcgtttaa atatgacgga ttgactacta actatgacgg attgactact aactatgacg
    12781 gattgactac ttaactatga cggattgact actaact
//
