LOCUS       NZ_AP024801            11158 bp    DNA     circular CON 16-MAY-2024
DEFINITION  Acinetobacter pittii strain OCU Ac17 plasmid pOCUAc17-3, complete
            sequence.
ACCESSION   NZ_AP024801
VERSION     NZ_AP024801.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00330122
            Sequence Read Archive: DRR301934, DRR301935
            Assembly: GCF_019703265.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter pittii
  ORGANISM  Acinetobacter pittii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1
  AUTHORS   Matsumoto,Y., Sakiyama,A., Tsubouchi,T., Suzuki,M., Niki,M.,
            Niki,M., Kakeya,H. and Kaneko,Y.
  TITLE     Complete Genome Sequence of Acinetobacter pittii OCU_Ac17, Isolated
            from Human Venous Blood
  JOURNAL   Microbiol Resour Announc 10 (39), e0069621 (2021)
   PUBMED   34591662
  REMARK    DOI:10.1128/mra.00696-21
REFERENCE   2  (bases 1 to 11158)
  AUTHORS   Tsubouchi,T., Sakiyama,A., Matsumoto,Y., Suzuki,M. and Kaneko,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUL-2021) Contact:Taishi Tsubouchi Osaka City
            University, Bacteriology; Abeno, Asahimachi 1-4-3, Osaka, Osaka
            545-0051, Japan URL :http://www.med.osaka-cu.ac.jp/bacteriology
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 101x
            Sequencing Technology             :: Illumina Miniseq; ONT Flongle
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019703265.1-RS_2024_05_16
            Annotation Date                   :: 05/16/2024 04:13:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,949
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,768
            CDSs (with protein)               :: 3,768
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 40 of 88
            Pseudo Genes (internal stop)      :: 17 of 88
            Pseudo Genes (multiple problems)  :: 13 of 88
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP024801.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11158
                     /organism="Acinetobacter pittii"
                     /mol_type="genomic DNA"
                     /strain="OCU Ac17"
                     /isolation_source="venous blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:48296"
                     /plasmid="pOCUAc17-3"
                     /geo_loc_name="Japan:Osaka"
                     /lat_lon="34.6466657 N 135.5087692 E"
     gene            1..930
                     /gene="repM"
                     /locus_tag="OCUAc17_RS19595"
                     /old_locus_tag="OCUAc17_38230"
     CDS             1..930
                     /gene="repM"
                     /locus_tag="OCUAc17_RS19595"
                     /old_locus_tag="OCUAc17_38230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065049.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_005065049.1"
                     /translation="MSELIVKDNALIQASYTLDTVEQRLILLAIAEARETGHGITENSL
                     LEVHASSYINTFNVEKHTAYTVLRDASKSLFDRYVTYHDINPKTGKDRSFHCRWVDKIG
                     YEPQSGIVFLRFTQDIVPLITRLEENFTKYELEQVSRLTSSYAIRLYELLIQWRSAGKT
                     PIFDLSIFRQQLGVEAHQYKTMSNFKTYVLDFALKQVNELTDITAKYEQHKKGRSISGF
                     SFSFKQKKTNSDKVIKGTDTLALFTKMSDKQRHLFANKLSELPEMGQYSEGTESFQQFA
                     IRIAEMLQDYQKFQELFPYLQKVGFAPA"
                     /name="repM"
     gene            1009..1539
                     /locus_tag="OCUAc17_RS19600"
                     /old_locus_tag="OCUAc17_38240"
     CDS             1009..1539
                     /locus_tag="OCUAc17_RS19600"
                     /old_locus_tag="OCUAc17_38240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017397202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_005065050.1"
                     /translation="MKSLTVLELSKLYNINRQTIYNNIKKGILSKNSQNKIDLAEAIRV
                     FGEPVKKQDVKEPVKIDSPNSAEVLLLRQQIDMLKNQLDDAKDRESFYQNQIETMQRLL
                     EAPKPNMTTFTDQELVQPSIAKPDPEPDTAEAKHDGLTTPERRENKWIPVPEHIEPEPE
                     KRGFWSRFFRPYD"
                     /name="plasmid replication DNA-binding protein"
     gene            1889..2206
                     /locus_tag="OCUAc17_RS19605"
                     /old_locus_tag="OCUAc17_38250"
     CDS             1889..2206
                     /locus_tag="OCUAc17_RS19605"
                     /old_locus_tag="OCUAc17_38250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000900860.1"
                     /translation="MLGKNHHFLKKFSISDAAIAYKKPRSTILKYIKSGDFELDYNKLI
                     EYSQLVRKFGELTYPIQDEREQQIKKLKDEVMHLKKQLNFFKYTSISNKNRLLISHKHS
                     I"
                     /name="hypothetical protein"
     gene            2727..3071
                     /locus_tag="OCUAc17_RS19610"
                     /old_locus_tag="OCUAc17_38260"
     CDS             2727..3071
                     /locus_tag="OCUAc17_RS19610"
                     /old_locus_tag="OCUAc17_38260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_005065055.1"
                     /translation="MWTVITTDLFNEWLEQQDEITQEKVLAALVVLQLQGPSLGRPLVD
                     TVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAIVLCIGDKSGKKRFYTEMLAIADKQ
                     YELHLSTLGE"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            3076..3378
                     /locus_tag="OCUAc17_RS19615"
                     /old_locus_tag="OCUAc17_38270"
     CDS             3076..3378
                     /locus_tag="OCUAc17_RS19615"
                     /old_locus_tag="OCUAc17_38270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_005065057.1"
                     /translation="MAKTLQELLANRSPESQARIQKMADELLLENQLYLIREELEISQK
                     ELAETLGIKQPSLSAIENRGNDLKISTMKKYVEAMGGKLRIDVELPTGKHIGFNV"
                     /name="helix-turn-helix domain-containing protein"
     gene            3470..3871
                     /locus_tag="OCUAc17_RS19620"
                     /old_locus_tag="OCUAc17_38280"
     CDS             3470..3871
                     /locus_tag="OCUAc17_RS19620"
                     /old_locus_tag="OCUAc17_38280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005065059.1"
                     /translation="MTDKKQNDHLNLDGINSSYNDGDGLRINNPEDFRSITISNGYFSN
                     NKGNGITIGSPQQSPLEIILTQLAPKLPDTIQPYELASVIQNLLESTNQEEISQKLMTS
                     GLKEKFKDPNLWISFSSLLFSLIFQFSSK"
                     /name="hypothetical protein"
     gene            3923..4597
                     /locus_tag="OCUAc17_RS19625"
                     /old_locus_tag="OCUAc17_38290"
     CDS             3923..4597
                     /locus_tag="OCUAc17_RS19625"
                     /old_locus_tag="OCUAc17_38290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="WP_005065062.1"
                     /translation="MSQKILQRYEQLLTDSASIKAMTKKAYSEYSGSYDTLGDEGDALY
                     LEWKVKVKNLLLLSCGEHSIHYRDFLDAEETQSFDTNTRIISRLIPILKASYDDFKNGF
                     LTSFKQIVQAEVFDSELEQARSLLSSGYKNSAAVIAGVVLETAIKELCLNNGIELEKKK
                     LTRLNEDLAKAGVYNPLQQKLITAMADIRNNAAHGDYDQFTKEDVHRMIEDIERFLLAY
                     SS"
                     /name="HEPN domain-containing protein"
     gene            complement(4688..4999)
                     /locus_tag="OCUAc17_RS19630"
                     /old_locus_tag="OCUAc17_38300"
     CDS             complement(4688..4999)
                     /locus_tag="OCUAc17_RS19630"
                     /old_locus_tag="OCUAc17_38300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_005065064.1"
                     /translation="MSMVRYTRKELNEKFSDKQDAEIKRLLAKGTVPDEQLDLSDIPEI
                     TDWSHAVRHGQFYRPVKQQTSVRLDADVLAWLKAQGKGYQTRMNKILREAMLEDLKNH"
                     /name="BrnA antitoxin family protein"
     gene            complement(4986..5273)
                     /locus_tag="OCUAc17_RS19635"
                     /old_locus_tag="OCUAc17_38310"
     CDS             complement(4986..5273)
                     /locus_tag="OCUAc17_RS19635"
                     /old_locus_tag="OCUAc17_38310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_002124950.1"
                     /translation="MELYFEWDEAKNQKNQKKHDVSFETASLVFEDPLRISIQDRHTNG
                     EERWQTIGMVKGVLMLLVAHTIIDDDDCEIIRIISARQVTKAERNQYEHG"
                     /name="BrnT family toxin"
     gene            5786..7273
                     /locus_tag="OCUAc17_RS19640"
                     /old_locus_tag="OCUAc17_38320"
     CDS             5786..7273
                     /locus_tag="OCUAc17_RS19640"
                     /old_locus_tag="OCUAc17_38320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953322.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="WP_005065066.1"
                     /translation="MLSTIREQWFSNIRGDVLAGIVVALALIPEAIAFSIIAGVDPKVG
                     LYASFCIAVVIAFVGGRPGMISGATGAMALLMVTLVKEHGLQYLLAATILTGFIQILAG
                     YLKLGALMRFVSKSVVIGFVNALAILIFMAQLPELTNVTWHVYAMTIGGLAIIYLFPYI
                     PVIGKLLPSPLICIVLLTLFALFIGLDVRTVGDMGQLPDTLPIFLLPDIPLNLETLTII
                     LPYSLGLAAVGLLESMMTATIVDDLTDTNSDKNRECKGQGVANIASGFLGGMAGCAMIG
                     QSIINVKSGGGTRLSTFSAGVFLLIMVVFISDWLKAIPMAALVAVMIMVAIGTFNWDSL
                     RNIRQYPLSSNIVMIVTVIVVVATHNLAYGVLVGVLLSALFFANKIERYMAIQSEFNEP
                     ENTRTYTVTGQVFFSSADKFTSAFDFKEALSKVVIDVSQAHFWDVSAVAAVDKVVIKFR
                     REGTEVELIGMNEASKTLVDKFGIHDKPDALDRLNSH"
                     /name="SulP family inorganic anion transporter"
     gene            7286..8137
                     /locus_tag="OCUAc17_RS19645"
                     /old_locus_tag="OCUAc17_38330"
     CDS             7286..8137
                     /locus_tag="OCUAc17_RS19645"
                     /old_locus_tag="OCUAc17_38330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034565.1"
                     /GO_function="GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="WP_005065068.1"
                     /translation="MSKIIACIDGSLATNTVCDYAAWFSDKLSSPLKLLHVIDKPKAKA
                     PQDLSGAIGLGSRETLLKELVEIEERKGKIELEHGQILLREAKNYLLEKFSIDAQSFQR
                     HGSLLETIMGMEEDIRVLVMGKHGAETEHDSSKVGTHIENVVRALHKPVLITSSPFSPP
                     KSFLIAFDGSPTARICVDKIANSPLLKTLTAHVVYVGKPNSDMTLQVTWAKELLANNGF
                     DVIDSMLDGDVEKSILDYQEKNVIDMIVIGAYGHSKIRQFFIGSTTTKLLSSSTKPVLL
                     LR"
                     /name="universal stress protein"
     gene            complement(8408..9292)
                     /locus_tag="OCUAc17_RS19650"
                     /old_locus_tag="OCUAc17_38340"
     CDS             complement(8408..9292)
                     /locus_tag="OCUAc17_RS19650"
                     /old_locus_tag="OCUAc17_38340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_005065070.1"
                     /translation="MFYLNLKHNIKKEQSSLNAFHYLTRTAHFENQKDNEKLEFMRYGN
                     MPKWAENKPKLFWKSADQFEISRGRTSSTLTIALPKELILEQRAELVQKLIDQFAGQYQ
                     FPYTAVIHNHPSEITGEDQPHLHLMYSERTISDDIERPPEQFFKQYRPKNPTQGGAQKL
                     TADALGFGRNQIKVFREKTQELMNLFLEKYAPTKKVMVYGVEIEVKNQVSCLSNDDFNQ
                     KFGTKLQDVPQIPRWKLYSADPIIQLDVEAQKNTIKKIRNQNNAELYGKEYDLEISRRH
                     VLTQKKDPGFSLD"
                     /name="MobA/MobL family protein"
     gene            9748..10461
                     /locus_tag="OCUAc17_RS19655"
                     /old_locus_tag="OCUAc17_38350"
     CDS             9748..10461
                     /locus_tag="OCUAc17_RS19655"
                     /old_locus_tag="OCUAc17_38350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006581718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005065071.1"
                     /translation="MAGRKTFALTQQEIIDLDKHIRALPFKRRMQDAFLAMISERESMK
                     QLNEEDLKLIQRCRYERNTYNKRIVQLQLIQKTEPQKLKKLELNILELSKKTDIDAYFA
                     MHDALNEVLKIKRNQTSEKNITAKVERVLNPEKYNQAPLSKSLRKQQDHIKYFIGSLYL
                     NLLEQSGLERSNSNEDLDRMLDLFKQAQSGAEKNNAFSLDQNRKTDILSAKSLYEMEKL
                     IKQAKEDPRNPFNKA"
                     /name="hypothetical protein"
     Typing          2..917
                     /name="rep_cluster_1226"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.884"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(8412..9292)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgagtgaat taatcgtaaa ggataatgct ttaattcaag ctagctatac cctagataca
       61 gttgaacaaa ggctgatcct attagccatt gctgaagctc gggaaacagg gcacgggata
      121 actgaaaata gccttttaga agtacatgca agtagttata tcaatacctt taatgtcgag
      181 aaacacactg cttataccgt gcttcgggat gcatctaaaa gcttgtttga tcgctatgtc
      241 acgtatcatg acattaatcc taagactggt aaggatcgta gctttcactg tcgttgggtc
      301 gataaaattg gatatgaacc tcaatccggg atcgttttcc tacgtttcac gcaagacatt
      361 gttccactca taactcgact ggaagaaaac tttactaaat atgaattgga gcaggtttcg
      421 agattaacta gctcttatgc tattcggtta tacgagttat taattcaatg gagatctgct
      481 ggtaaaacgc caatttttga tctatcgatc tttagacaac aacttggtgt cgaagctcat
      541 caatacaaaa caatgagtaa ttttaaaacg tatgttttag attttgccct caagcaagta
      601 aatgagttaa ccgatataac ggctaaatat gaacaacata aaaaaggacg ttcaatttcc
      661 ggtttttctt tcagttttaa gcagaaaaaa accaactctg acaaagtaat taagggaacg
      721 gatacattag cccttttcac caaaatgagt gataaacaac gtcatctctt tgccaacaaa
      781 ctttctgaat tacctgagat ggggcaatat tcagaaggca ccgaaagctt ccaacaattt
      841 gctatacgca ttgcagaaat gctccaagat tatcaaaaat tccaagaatt attcccttat
      901 cttcaaaaag ttggctttgc acctgcctaa aatttaaact tgatcatttg tttattagac
      961 agtctaatag acaacaactt ttgacaggtt taggcataga tttacaacat gaaaagcctt
     1021 acagttctcg agctttctaa gctttacaat atcaatagac aaacgattta caacaacata
     1081 aagaaaggaa ttttaagtaa aaattcccag aataagatcg atctagccga ggctatccgt
     1141 gttttcgggg aacctgtcaa aaaacaggat gtaaaagaac ctgtaaaaat agacagtcca
     1201 aattcggcag aagttttact acttagacag cagatagaca tgctgaaaaa ccagcttgat
     1261 gatgccaaag atcgggaatc tttctatcag aaccaaattg aaacgatgca acgcctatta
     1321 gaagccccaa aacctaatat gactacgttt accgatcaag aattagtgca acccagcata
     1381 gccaaaccag atcccgaacc tgacacagca gaagctaaac atgacggatt gactactcca
     1441 gagcggaggg aaaataagtg gattccagtg ccagagcaca ttgaaccaga gcctgagaaa
     1501 agaggctttt ggagccgttt ttttagaccc tatgattaac cacgattgtt taattaatat
     1561 gaataaagtc gtgaaacttt gattaaataa taagcgtgga acgtgaaaaa tcataaaaaa
     1621 aaagcccacc ttggggaggt tgggcaaaaa agaaactaca gcgttgattt ctaaggaaaa
     1681 ttataacaca tttcgtataa ggtgtattat gttaataata taaattttta aaagcatatt
     1741 gatttaataa tagaaaaatt atcagagtag ggcatgtgta aagtaaacgt tcgattatta
     1801 accaataatg attaaagatt tatatcttgt atgtgttaat tttattaaca tatatatacg
     1861 aaattaataa tgccttaagt gaaatagtat gctaggtaaa aatcatcatt ttttaaaaaa
     1921 attttcgatc agtgatgctg ctatagcgta taaaaaacct cgctcgacta tacttaaata
     1981 tataaaaagc ggagatttcg aacttgacta caataaattg attgaataca gtcagttagt
     2041 tagaaaattt ggtgaactta cttaccctat acaggacgaa agagagcaac aaatcaaaaa
     2101 actaaaagat gaagttatgc atttaaaaaa acaacttaac ttttttaaat atacttcaat
     2161 ctcaaacaaa aatagactat taatttctca taaacattcc atttaacata atagagctta
     2221 tacgaaatgc tttttaaaat ctccttgttt ttcatatagt taaaaatttt tcatccccct
     2281 aaaaaaccac aaaaaaccga ctgggaaaca agtcggtttc tttttaagca aaactgtaac
     2341 ttttagccaa taaaatcgat caaaaaacat tcgtttttca ttttttcctg atttctcaat
     2401 ggtcacactt cacaaataat ccccaatttc tgcggacaac tcttgggata aaataggcca
     2461 actttgtaag ctaaagctta cagagattta aggattttgc tgattggtaa tccaaggtac
     2521 tagccctatt atgaatacat aagggaactg gatgtctcca gacaacaaga ataagaaaat
     2581 agcgcaagga atgggcagca cgagatcagt aagacgagca cccgatatga aaaagcccga
     2641 caggatgtcg ggctttttta ttgccatttg caaagttatt caatcagctt taatatatag
     2701 actataatct atattatggt tgtaatatgt ggacagtcat tacaacggat ctgtttaatg
     2761 aatggcttga acagcaagat gaaataacac aagagaaagt cttagctgcg ttggttgttc
     2821 tgcaactgca aggaccaagt ttaggtcgtc ccttagtaga taccgtctac gattctaaat
     2881 ttaccaatat gaaggaacta cgtgttcagc atcgaggtaa accattaaga gcattttttg
     2941 cattcgatcc attacgacaa gcaattgtac tgtgtattgg tgataagagc ggtaaaaagc
     3001 gtttttatac agagatgcta gcaattgcag ataagcaata tgaacttcat ctctcaacat
     3061 taggagaata atctgatggc taagacccta caagaattat tggctaatcg ctctcctgag
     3121 agccaagccc gtattcaaaa aatggctgat gaattgctat tagaaaatca gctttacctt
     3181 attcgtgaag aactcgaaat ctctcaaaaa gaactggctg aaactttggg aataaaacag
     3241 ccatcgcttt cagcaataga aaatcgtggc aatgatctta agatttcaac catgaaaaag
     3301 tatgttgaag ctatgggagg aaagctccgt attgatgtgg aacttccaac tgggaaacat
     3361 ataggattca atgtatagat atagaaaagt gagtttaagc tcactttttt catttggtct
     3421 gatcagtatg atataagtta taaatcattc aaaagataaa ggcttatata tgactgataa
     3481 aaaacagaac gatcatctaa atcttgatgg aatcaactca tcttataatg atggtgatgg
     3541 gctaagaata aataatcctg aggatttcag aagtattacc atatctaatg gctatttctc
     3601 aaacaataaa gggaatggaa ttactattgg ctcaccgcaa caatcaccat tagaaatcat
     3661 tcttactcaa ttagccccaa aacttccaga tactattcag ccttatgaat tggcatcagt
     3721 tattcaaaac cttctggaat caactaatca agaagaaata tctcaaaaat taatgacttc
     3781 aggccttaaa gaaaaattta aagatcctaa tctttggata tctttcagta gtttattgtt
     3841 ttcattaatt ttccaatttt catctaaata gatttataga gttcaattac ttaatttgga
     3901 aaccctaaaa aactgagtaa acatgagtca aaaaatatta cagcgttatg aacaactact
     3961 aaccgattct gcttccatca aagctatgac aaaaaaagct tactcagaat atagcggtag
     4021 ctatgatact ttgggtgatg agggggatgc tctttatctt gaatggaaag ttaaagttaa
     4081 aaatctatta ttactatcat gtggtgaaca ttctattcat taccgtgatt ttttagatgc
     4141 tgaagaaaca cagagctttg atactaatac tagaataatc agtaggttaa ttccaatttt
     4201 aaaagcatcg tatgatgatt ttaaaaatgg ttttttaact tctttcaagc aaattgttca
     4261 agctgaagtt tttgatagtg aattagaaca agcacgatcg ctgttaagta gtggttataa
     4321 aaattcagct gctgtaattg ctggtgtagt tctagaaacc gcaattaaag aactatgttt
     4381 gaataatgga atagaattag agaagaaaaa attaacacga ttgaatgaag acttggcaaa
     4441 agcgggagtt tataaccctc tacagcaaaa actaattaca gctatggctg atattcgaaa
     4501 taatgcggcc catggagatt atgatcagtt tacaaaagaa gatgttcaca ggatgatcga
     4561 agatatcgaa agatttctac tagcttactc ttcgtaagac tgtcagattt acatagaaaa
     4621 aatttaatta tttaacataa aatctcttat gcgaagcaaa aatgggagtc tatagctccc
     4681 attttcatca atgattctta agatcttcta gcattgcttc acgcagaatt ttattcatgc
     4741 gtgtctgata gcctttgcct tgagccttta accatgcaag tacatctgca tctagacgaa
     4801 cagatgtttg ttgcttcact ggacgataaa attgcccatg gcgaacagca tgactccagt
     4861 cggtaatctc aggaatgtca gatagatcta attgttcatc tggtacagtt ccttttgcaa
     4921 gcaagcgttt aatttcagca tcttgcttgt cactgaattt ttcattcagt tctttgcgtg
     4981 tatatctaac catgctcata ttgattgcgt tccgctttag tgacttgcct tgcactaatg
     5041 attcgtatga tctcacagtc atcatcgtca atgattgtat gagccaccaa taacatcagt
     5101 acacctttaa ccataccgat cgtttgccaa cgttcttcac cattggtatg tcgatcctgg
     5161 attgagatcc gtaaaggatc ttcaaaaacg aggcttgccg tttcaaaaga tacatcatgt
     5221 tttttctgat tcttttggtt tttagcctca tcccattcga aatacagttc cataaaaaac
     5281 attcaaattt gtatatacaa taatgtatca caaatttgta tcactcaagc taattggagt
     5341 aatttttagt caaaattatt ggcaagaagt atcaaacttg ctcaaaaaaa atccgctttg
     5401 tttccaaagc ggatttttgc ggtggttacg ggtgatgtaa aatagttaag catatgattt
     5461 tatttaactt ataaaagacg atttcggata acgcccatta tgttaaatgg gcataaatca
     5521 attctaaaat tatctatgat aattcttagc ttttacttca gtactgcgtt tagggtggat
     5581 agaaaagggg ctagctgcta tgattgcccc cgaaatcctc caaaatagat atctttctta
     5641 atatgacact tacgccacca taagtttatt tacctcttaa atcagtaaat aactcatcac
     5701 tgtctagtga tgtggtgtag tagcttgtgt gtcgtatata tcacatcact agcctttctt
     5761 aaaatttaaa aaaataggct atttcatgtt atccactatt cgagaacaat ggttctctaa
     5821 tattcgcggg gatgtccttg cgggtattgt tgtcgctttg gctctaattc ctgaagcaat
     5881 tgccttctct attattgcag gtgtcgatcc taaagtcggg ttatacgctt cattctgtat
     5941 tgccgtggtg attgctttcg taggtggtcg ccctggtatg atttctgggg caaccggtgc
     6001 catggcgtta ttaatggtaa cactggttaa agagcatggt ctgcaatact tattagcagc
     6061 cacaattttg acaggattta ttcaaatctt agccggctat ctaaaattag gtgccttgat
     6121 gcggtttgta tccaagtcag ttgtgattgg ctttgtgaat gccctggcta ttctaatttt
     6181 tatggctcaa ttacctgaac tcactaatgt gacatggcat gtctatgcga tgacaattgg
     6241 tggtttagcc attatttatc tattccctta tattccagtg atagggaagc ttttaccctc
     6301 tcctctcatc tgtattgtct tacttacgct ctttgcctta ttcattggtt tagatgtaag
     6361 aaccgtcgga gatatggggc aattaccgga tacgcttcca attttcttgc ttcctgatat
     6421 tcctttaaat cttgaaacct taacgattat tttgccttat tcactgggat tagcagccgt
     6481 aggtttgctt gaatctatga tgacagcaac gattgtcgat gatttaacag ataccaatag
     6541 tgataaaaac cgtgagtgta aaggacaagg cgttgccaat attgcttcag gtttcttagg
     6601 cggaatggca ggctgtgcca tgattggtca atcgattatt aatgtgaaat ctggtggggg
     6661 tacccgttta tctacattta gtgcaggggt ctttctgtta attatggtgg tgtttatcag
     6721 tgactggctt aaagccattc caatggctgc actggttgct gtcatgatca tggtagcaat
     6781 tggaacattt aactgggatt cactacgcaa tatccgccaa tatcccttat ctagcaatat
     6841 tgtgatgatc gttacagtca tcgtggtcgt tgcgacacat aatttggctt atggcgtatt
     6901 agttggtgta cttctttctg cattattttt tgccaacaaa attgaacgct acatggcaat
     6961 tcagtccgaa tttaatgaac ctgaaaatac ccgtacttat acggtaacag gtcaagtgtt
     7021 cttcagctca gcagataaat ttacttctgc atttgatttc aaagaagcac tttcaaaagt
     7081 tgtaattgat gttagtcagg ctcacttttg ggatgtttct gcggtcgctg ccgtagataa
     7141 agtggtcatt aagtttagac gtgaaggaac agaagtcgaa cttattggaa tgaatgaagc
     7201 cagcaaaacc ttagtcgata aatttggcat ccatgataaa ccagatgcat tagatcgctt
     7261 aaatagccac taagagggat gaaaaatgtc aaaaattatt gcatgtattg atggatcatt
     7321 agcaacaaat accgtttgtg attatgcagc ttggtttagc gataaactta gttcaccgct
     7381 taagttatta catgttattg ataagccaaa agcgaaagca ccacaagatt taagcggtgc
     7441 aatcggcttg ggaagtcgag aaaccttgct caaagaattg gttgaaattg aagagcgtaa
     7501 ggggaagatt gaactagagc atggccaaat tcttcttcgg gaagcaaaga attatctatt
     7561 ggagaagttc tctattgatg ctcaaagttt tcaacgccac ggcagtcttt tagagacaat
     7621 catgggaatg gaagaggata ttcgtgttct tgttatgggt aaacatgggg ctgaaaccga
     7681 gcatgattcc agtaaagttg gaacccatat tgaaaatgtt gttcgtgcac ttcataaacc
     7741 cgtattaata acatcttcac cctttagtcc acctaagtct tttttaattg cttttgatgg
     7801 aagtcctact gcacgtattt gtgtagacaa aattgcaaat agccctttat taaaaaccct
     7861 gacagctcat gtggtatatg ttggtaagcc aaatagtgat atgacattac aagtcacttg
     7921 ggcaaaagag ctacttgcaa ataatggttt tgatgtaatt gatagcatgc ttgacggtga
     7981 tgttgagaaa tctatattgg attatcaaga aaaaaacgtt attgatatga ttgtaattgg
     8041 tgcatatggt cattcaaaga tacgccagtt ttttattggt agtacgacga ccaaattgtt
     8101 atcaagctct acaaaaccag tattattatt aagataattt ttaaaagata aaaggatagt
     8161 ttttaactat ccctttttta gattttctaa aattaacata atacacctta tgcgaaaaac
     8221 ctagatttta catttatggg caaaaatcta ggttttcctt attgacctga atcaaaaaca
     8281 caatctatcc atcattgcag cctaaaaaac acaaattttc catgttgtgg ctaaaaatca
     8341 ctcacaatat ggataagttt tttcaagatt ccatgttcaa aaaacacaaa ttttccactt
     8401 ataaaaatca atctaagcta aagccaggat cttttttctg agtaagcaca tggcgtctag
     8461 atatttctaa atcatattcc ttgccataaa gttcagcatt gttctgattt ctaatttttt
     8521 taattgtgtt tttttgagct tcaacatcaa gctgaataat tggatcagcg ctataaagtt
     8581 tccaacgagg gatttgtggt acatcctgta atttggtccc gaatttttga ttgaaatcgt
     8641 cattagatag gcaactgacc tgatttttca cttcaatttc tacaccataa accattactt
     8701 ttttagttgg tgcatatttt tctaaaaaca aattcattaa ttcttgagtt ttttcacgga
     8761 atactttgat ttgatttcgc ccaaaaccta atgcatcagc tgttaacttt tgcgcaccac
     8821 cttgagttgg attttttggc cggtactgtt taaaaaactg ctctggtggt cgttcaatat
     8881 catctgaaat tgttctttct gaatacatca aatgtaaatg tggttgatcc tctccagtaa
     8941 tttcactggg atgattatga atgactgcgg tataaggaaa ttgatattgt ccggcaaact
     9001 gatctattaa cttctgaacc aattctgctc gttgttctag tattaactct tttggtaaag
     9061 caatggttaa agtagagctt gttcttcccc gggaaatttc aaattgatca gcacttttcc
     9121 aaaaaagttt tggtttattt tctgcccatt tgggcatatt tccataccgc ataaactcta
     9181 atttttcatt gtctttttga ttttcaaaat gtgccgtccg agttaaataa tggaatgcat
     9241 tcagagaact ctgctctttt ttaatattat gctttagatt taaataaaac atcagttcac
     9301 ctcaaacaaa ttacgtgtaa cgaaacagca aattttcttg taacaagggg ctaattcttg
     9361 ttgcagagag cagtctcttc ttgttgcaaa catcgatatt tcatcaaaaa agtttgttgc
     9421 actcgtctaa aattcgctgt tttccttgtt gcaccccttt cccatttctt gtaacggcag
     9481 acattgctta ctttttcacc attaagaaaa ataccgttaa aacccgaaga aaaactcata
     9541 atttaataat taaatcagtg gtttatgtag cctccgcagg agcgagtaga agtgtctttt
     9601 tttaacaatg attgctaaag caatcattgt taaaaaaaga cacggaaacg agataaacaa
     9661 tattttatgt ataacataaa atattgtttc aacaccatca gaattccgct aacatcaatt
     9721 tagataccca aaatcaggaa atccgctttg gccggtagaa aaacatttgc cttaacacaa
     9781 caagaaatca tcgatttgga taaacatatt cgagcgctgc cgtttaaaag acgaatgcaa
     9841 gatgcttttt tagcaatgat ttctgaacga gaatcaatga aacaactgaa tgaagaagat
     9901 ttaaaactta ttcaacgatg tcgttatgag cgcaatactt ataataaacg tatagttcaa
     9961 ttacaactga tccaaaaaac cgagccacag aaattaaaaa aattagaatt gaatattcta
    10021 gaattaagta aaaaaacaga tattgatgct tactttgcaa tgcatgatgc attaaatgaa
    10081 gttttaaaaa ttaaaagaaa tcagacttct gaaaagaata ttacagcgaa ggtcgaacga
    10141 gttttaaacc ctgagaaata taaccaggcc ccccttagta aatctttaag aaaacagcaa
    10201 gaccacatta aatattttat cggatcacta tatttaaact tattggaaca aagcggttta
    10261 gagcgatcaa attcaaacga agatttagat cgaatgctcg atttatttaa gcaggcacaa
    10321 tccggagcag aaaagaacaa tgcatttagc ctggatcaaa atagaaaaac agatatttta
    10381 tcggctaaat cgctatatga aatggagaaa ttgattaagc aagccaaaga agatccaagg
    10441 aatcctttca ataaagctta gattgactca aataataggg tttaaataca ctgttgaagc
    10501 cgtatatgcc ttttttagcc taaacctata ttccccatca ataaaaaaat tagagtgctc
    10561 taaacgcaaa ttagagtatt ttggagatca tttgatttta ccgataagaa caaagcgagt
    10621 gtctacgagc gatccttgga aaattttgcc tagactccct gaaaaaccgc tttttaggct
    10681 gtgaaaccta aataagcatg gaaaagttgt agaaatttga gtctagatcg agcgtagcga
    10741 gtaaaaaagt tttatgagcg tagcgaattc cgagttgctt ttgctttttt aaagtcacgt
    10801 aagtgctcac caaaaaattg ccccgacgaa ctgagcgaaa gcgaagttca atagagtttg
    10861 agcgaagcga aaaccaaggg caatttttct ttacctgggc ttttaattat ttttaatctt
    10921 ttaaatgctt ttaagtaagc tgtaaccctt tattcataat agttttagag ttaataaaac
    10981 taggtttatc gacccataaa actaggttta tcgacccata aaactaggtt tatcgaccca
    11041 taaaactagg tttatcgacc ttaaaactag gtttatcgac cccaaattaa gtcgatttac
    11101 aattcaatta aaatagtcta ttgtacaacc tagtttaatt ataaatttat tattagaa
//
