LOCUS       NZ_AP024693             1459 bp    DNA     circular CON 04-NOV-2023
DEFINITION  Escherichia coli strain FUJ80154 plasmid pFUJ80154-6, complete
            sequence.
ACCESSION   NZ_AP024693
VERSION     NZ_AP024693.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00317522
            Assembly: GCF_019973795.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Suzuki,M., Harada,Y. and Doi,Y.
  TITLE     Complete sequence of Escherichia coli strain FUJ80154
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1459)
  AUTHORS   Suzuki,M., Harada,Y. and Doi,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAY-2021) Contact:Masahiro Suzuki Fujita Health
            University, School of Medicine, Microbiology; Dengakugakubo 1-98,
            Kutsukake-cho, Toyoake, Aichi 470-1192, Japan URL
            :http://www.fujita-hu.ac.jp/~microb/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9b
            Genome Coverage                   :: 108x
            Sequencing Technology             :: MiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/04/2023 03:00:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,190
            CDSs (total)                      :: 5,074
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 222
            CDSs (without protein)            :: 222
            Pseudo Genes (ambiguous residues) :: 0 of 222
            Pseudo Genes (frameshifted)       :: 85 of 222
            Pseudo Genes (incomplete)         :: 150 of 222
            Pseudo Genes (internal stop)      :: 45 of 222
            Pseudo Genes (multiple problems)  :: 53 of 222
            Pseudo Genes (short protein)      :: 4 of 222
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP024693.1.
            Annotated by DFAST v.1.2.13 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FUJ80154"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pFUJ80154-6"
                     /geo_loc_name="Japan:Aichi"
                     /collection_date="2019-08-21"
     gene            796..1236
                     /locus_tag="FJMB_RS25875"
                     /old_locus_tag="FJMB80154_p610"
     CDS             796..1236
                     /locus_tag="FJMB_RS25875"
                     /old_locus_tag="FJMB80154_p610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000031238.1"
                     /translation="MSKDVERITQINDQGEIIGGFVAVIRPKQKSAFQRHFTMNQDALR
                     ILAKELTGEQFKVLMLMLADLDYENFIQIAQADIAETLGMQKTNVSRAVRALLDVGVIF
                     EGPKVGRSKTYRLNEQFGWKGTVTNHKKALKNGFSVIQGGRI"
                     /name="hypothetical protein"
     Typing          387..467
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.902"
                     /coverage="87.0"
                     /evalue="4.46e-26"
     Typing          complement(12..272)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.13"
                     /coverage="100.0"
                     /evalue="1.84e-104"
ORIGIN
        1 accacctacc aaccgagtgg ctgcttgtac cgtgcaaatg ggctttgcag ggggctggcg
       61 cggttcctgg cgacctgaaa gcccctggct aggggtatcg gcgcgcctga cctcctgaaa
      121 cgctctgtaa gcgtttttcg cgccttccat gccctgacgg gcatttaaac cccgcctcgt
      181 gcgtctgaga gcttctgagc gcgtttgaag ggctatctga cgactcatcc ccgaaagaac
      241 aacagcggaa tcaccgacag catgcgagag gtggcaccgc tcgccgcagc cccagcgacc
      301 gaacgttagt gagcgagtgg gcgaggaagc ggaagagaac aggaaaccac attgagcacg
      361 tacgcactga tacggggtgt cggggcgaag ctctgaccag atggcaaacg gaatatcgtc
      421 gcgggtgacg gtattacatt tgcacatcct gtcctgattt ctgaggcgtt ttaacggtga
      481 acggacggca aaaacatgat ggagctgttg aacgtggaga gcgagaaatc ggacaaaaat
      541 catggctgac gaggcttgca gaggagtaag ccagtataca ctccgctatc gctacgtgac
      601 tgggtcaggg cttcgccccg acaccccaaa gagcagctag gataaatatc taatggaatc
      661 aatgcgttag acgcctaata taaagataca gaaaggagaa taaaaacatt aataaaatct
      721 aagggttaca gttgacatag ttatcaataa tgataacact gttatcatta ttgataactc
      781 aaatagggtt caaagatgag taaggatgtc gagagaatca ctcagataaa tgaccagggt
      841 gagattatcg gcggcttcgt tgccgttatc cgccctaaac aaaaatcagc atttcagagg
      901 catttcacca tgaatcaaga cgcattacgg atcttggcta aggaattaac cggggagcaa
      961 ttcaaggtct taatgctgat gcttgcagat ctggattacg aaaacttcat tcagatcgcc
     1021 caagcagata ttgctgaaac tttagggatg cagaaaacga atgttagtcg cgccgttcgc
     1081 gcgctactgg atgttggggt gatctttgag ggacctaaag tcggcagatc aaaaacatac
     1141 cggctgaatg aacaatttgg ttggaaaggc accgtaacca accacaaaaa ggcactcaaa
     1201 aacggcttta gtgtcattca gggcggccgg atttaaagag ccagcggcag gcggacaatc
     1261 cacagaacat tgcatgcaat ttctagtgga ttgtctatcc tgcattgctc ttttgccgcc
     1321 tcaaaatccc ttgcgtgttt ttgctccccg atctccagaa cgaccgagcc gccacggttc
     1381 cggcagcgcc ttgagcgcaa ccggaaaccg tctggggaga cccccagacc ccccatcgct
     1441 gtaacaccga cggtctacg
//
