LOCUS       NZ_AP024692             3258 bp    DNA     circular CON 04-NOV-2023
DEFINITION  Escherichia coli strain FUJ80154 plasmid pFUJ80154-5, complete
            sequence.
ACCESSION   NZ_AP024692
VERSION     NZ_AP024692.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00317522
            Assembly: GCF_019973795.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Suzuki,M., Harada,Y. and Doi,Y.
  TITLE     Complete sequence of Escherichia coli strain FUJ80154
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Suzuki,M., Harada,Y. and Doi,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAY-2021) Contact:Masahiro Suzuki Fujita Health
            University, School of Medicine, Microbiology; Dengakugakubo 1-98,
            Kutsukake-cho, Toyoake, Aichi 470-1192, Japan URL
            :http://www.fujita-hu.ac.jp/~microb/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9b
            Genome Coverage                   :: 108x
            Sequencing Technology             :: MiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/04/2023 03:00:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,190
            CDSs (total)                      :: 5,074
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 222
            CDSs (without protein)            :: 222
            Pseudo Genes (ambiguous residues) :: 0 of 222
            Pseudo Genes (frameshifted)       :: 85 of 222
            Pseudo Genes (incomplete)         :: 150 of 222
            Pseudo Genes (internal stop)      :: 45 of 222
            Pseudo Genes (multiple problems)  :: 53 of 222
            Pseudo Genes (short protein)      :: 4 of 222
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP024692.1.
            Annotated by DFAST v.1.2.13 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FUJ80154"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pFUJ80154-5"
                     /geo_loc_name="Japan:Aichi"
                     /collection_date="2019-08-21"
     gene            complement(11..123)
                     /locus_tag="FJMB_RS26050"
                     /pseudo=""
     CDS             complement(11..123)
                     /locus_tag="FJMB_RS26050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(777..971)
                     /locus_tag="FJMB_RS25860"
                     /old_locus_tag="FJMB80154_p510"
     CDS             complement(777..971)
                     /locus_tag="FJMB_RS25860"
                     /old_locus_tag="FJMB80154_p510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041633.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHELA
                     EELQNSIQVRFETESETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2116..2397)
                     /locus_tag="FJMB_RS25865"
                     /old_locus_tag="FJMB80154_p520"
     CDS             complement(2116..2397)
                     /locus_tag="FJMB_RS25865"
                     /old_locus_tag="FJMB80154_p520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000586174.1"
                     /translation="MIINIKHKGLRKFAETGNNAGIPQQFTQRLKLILTALSTATNLQD
                     MMDLKALRCHPLKGDRQGEYSLEVNGNWRVVFKLEEPNVFDVDLEDYH"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            2742..3038
                     /locus_tag="FJMB_RS25870"
                     /old_locus_tag="FJMB80154_p530"
     CDS             2742..3038
                     /locus_tag="FJMB_RS25870"
                     /old_locus_tag="FJMB80154_p530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609270.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_000609270.1"
                     /translation="MINLVDPVHPGVFIREMFMEPFEISAADLSEKLHVSPSTLSRVLN
                     GKADLSVEMALRLSRVLGRSAESWINMQTQYSLNASRKSPNVIKELDELVPIF"
                     /name="HigA family addiction module antitoxin"
     Typing          622..704
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.79e-36"
     Typing          1008..1106
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.22e-45"
     Typing          1231..1487
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.139"
                     /coverage="89.0"
                     /evalue="1.57e-115"
     Typing          complement(52..609)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.286"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctcctctaaa tcagttacca gtggctgctg ccaatggtgt tttgcatgtc tttccgggtt
       61 ggactcaaga tgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
      121 cacacagccc agcttggagc gaacgaccta cactgaaccg agatacctac agcgtgagct
      181 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
      241 ggtcggaaca ggagagcgca cgagggagcc accaggggga aacgtctggt atctttaagt
      301 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttc tgtgatgctc gtcagggggg
      361 cggagcctat ggaaaaacgc ctgcggcgcg gccttcttcc tgttcttggt cttttgctca
      421 catgttcttt ccggccttat cccctgattc tgtagataac cgtgctgccg tttttgtgta
      481 agtcagtacc gctcgccgca gccgaacgac cgagcgtagc gagtcagtga gcgaggaagc
      541 ggaaaagcgc ctggacgcgc attttctcct tacgcatctg tgcggaattt cacacccggc
      601 atggggcact tttcatacaa tctgcactga tgccgcatag ttaagccagt atacactccg
      661 ctatcgctac gtgactgggt cagggcttcg ccccgacacc cgctgacgcg ccctggcggg
      721 cttgtctgct gccgaaaacg ctcgcggaca agccgtgagc gcctccgtca gcttcgttat
      781 gttcctgttt cactttcggt ctcaaagcga acctggatgc tgttctggag ttcttccgcg
      841 agttcgtgca gtcgttcgca catggccgcc tgttcgtcgg catccagagc atccagtttt
      901 tcaagcagtg tcaggctctg gcttttgata aatcgcgcca tattcagcgc agtttgttgc
      961 tgtttgttca ccttccgttg ttctccgtca gtaccgggtg tcgggtggac cctgaccagg
     1021 tggtaattgt aagacgttgc gggcgacggt attacaattg cacatcctgt cctgtttttt
     1081 taggacgtat catggcacag catatcaact gataaataat ccctgtgcag tgcagccgct
     1141 gttgcataaa atttcgctct gcgtcgagtg tttcttaatc aataattccg ctgatactcc
     1201 ggaaaaacgc gagtctgttc tcatgcctga aatgcccaca ccccacgcaa aaacaatttt
     1261 ttgctgattt ttctttataa atagagagtt atgacaaatt agttcttctt gctctctttg
     1321 tgatatttaa aaaagcggtg tcggcgcggt gttgtagctg cgccaacacc gctttttagg
     1381 gggtggtact gactattttc ataaaaaaca tcattttata ttaggggtgc tgctagcggc
     1441 gcggtgtgtt tttttacagg acgcccctgg gggcgctgct aggggtgtct gttcagatat
     1501 gtagcaatga gtcagggatt cacgaactga aaatgtccga aaatgtgtct catgggcacc
     1561 gtatgcgccc aaaccgatcg ccattcactt tcatgcatag ctatgcagtg agctgaaagc
     1621 gatcctgacg catttttccg gttttcgctt ggtaaaacat ctctttttgc ggtgttcgcg
     1681 gcagaatcgc gttcagcgcg ttttagcggt gcgcgtaatg cgacgttatg gtaaatgctg
     1741 tgatttacgg gatggtttta tctatgttga tatcaatacc acctcctatg gaggtggctt
     1801 acaccgattt ttccttgttg tctgtttcag aacagataga ggcttttttg gcaattatcc
     1861 aaccactatt gatagtgttt attactactt agataactga gcctttactg tatctgcccc
     1921 tgcaagcagg aacccaacag ttaagaacca cccccagcca ttgatacctt ttagcgctag
     1981 aattgctgcg ctaatgatgg ctgctatagg gactatctgg tacagaaaat acttcatata
     2041 acttaccccg aggttttgct tttttatgag accctaaagc atcgatactg tcactttgat
     2101 gctttaagga ccttcttagt gataatcctc gagatccacg tcaaagacgt taggttcttc
     2161 gagcttaaac actacgcgcc agtttccatt gacctctaac gaatactctc cttgacggtc
     2221 tcccttcaat ggatggcagc gcaatgcctt taaatccatc atatcctgca agttcgtcgc
     2281 tgtgcttaag gctgttagga tcagcttcag acgttgagta aactgttgcg gaattcctgc
     2341 gttgttacct gtttcggcaa acttacgcaa acctttatgc ttgatattta tgatcattaa
     2401 tcaccatccc atatttgttt agatagaaac atcgcagttt atttttccgg ttcatccgta
     2461 tttccattgc aaaccccttg ctctatttga ataaatgacg taggttttac ttaaccaagt
     2521 cgcattttaa tctgttgaca ttggtttgat gcgctgtaca atgccggggc cttgaatttc
     2581 acatcaagta cccacatcat aaggaatgat tagacgcacc aatgtgcatc ataatgtcat
     2641 tctttttttt agtcaacaat gagttgcgtt atgcgcaatg cgcaatctga aaacgcaata
     2701 ggcctatctt tttaaaacta atataggctt cagaggaagg tatgattaat cttgtcgatc
     2761 cagtacatcc aggggttttt atccgtgaga tgtttatgga accgtttgaa atatcagccg
     2821 cggacctgtc tgaaaaactg catgtatcac cttccactct aagtagagtg ctgaatggaa
     2881 aagctgattt aagtgttgag atggcattaa ggctttctcg agttcttggt cgcagtgccg
     2941 agtcgtggat aaacatgcag actcaataca gtcttaacgc gagcaggaag tcacctaatg
     3001 tcattaagga attagatgaa ttggttccta tattttgaga aaagaaaagg ccgctatatg
     3061 cggccttttt ctgtcttgac tctcaggcaa ctggatcgta tcgtttctcg taaacgaaaa
     3121 aaccacccgg caaggtggtt ttttcgaagg ttcagtaacc ggcaagtttc tgaaccaggg
     3181 taactggctt ggaggagcgc agtcaccaaa acttgttctt tcagtttagc cttaactggc
     3241 gcataacttc aagactaa
//
