LOCUS       NZ_AP024691             4074 bp    DNA     circular CON 04-NOV-2023
DEFINITION  Escherichia coli strain FUJ80154 plasmid pFUJ80154-4, complete
            sequence.
ACCESSION   NZ_AP024691
VERSION     NZ_AP024691.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00317522
            Assembly: GCF_019973795.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Suzuki,M., Harada,Y. and Doi,Y.
  TITLE     Complete sequence of Escherichia coli strain FUJ80154
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Suzuki,M., Harada,Y. and Doi,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAY-2021) Contact:Masahiro Suzuki Fujita Health
            University, School of Medicine, Microbiology; Dengakugakubo 1-98,
            Kutsukake-cho, Toyoake, Aichi 470-1192, Japan URL
            :http://www.fujita-hu.ac.jp/~microb/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9b
            Genome Coverage                   :: 108x
            Sequencing Technology             :: MiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/04/2023 03:00:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,190
            CDSs (total)                      :: 5,074
            Genes (coding)                    :: 4,852
            CDSs (with protein)               :: 4,852
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 222
            CDSs (without protein)            :: 222
            Pseudo Genes (ambiguous residues) :: 0 of 222
            Pseudo Genes (frameshifted)       :: 85 of 222
            Pseudo Genes (incomplete)         :: 150 of 222
            Pseudo Genes (internal stop)      :: 45 of 222
            Pseudo Genes (multiple problems)  :: 53 of 222
            Pseudo Genes (short protein)      :: 4 of 222
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP024691.1.
            Annotated by DFAST v.1.2.13 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FUJ80154"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pFUJ80154-4"
                     /geo_loc_name="Japan:Aichi"
                     /collection_date="2019-08-21"
     gene            321..1013
                     /locus_tag="FJMB_RS25845"
                     /old_locus_tag="FJMB80154_p410"
     CDS             321..1013
                     /locus_tag="FJMB_RS25845"
                     /old_locus_tag="FJMB80154_p410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281403.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCISRLKSGADAELQKT
                     MTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKNDEKREEFRWIQYRAQYAKG
                     EAKARITFSDAVMPYLTQLKGQFTRVVIKNISGLSSSYSIRIYEFLQQFRSTGERIIAL
                     NDFRSMLGIENKYKQFRDLNKILIKPCVDELNKKSDLAVTVETIKKGRTVVALHFRFKE
                     DKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(1271..3430)
                     /gene="mobQ"
                     /locus_tag="FJMB_RS25850"
                     /old_locus_tag="FJMB80154_p420"
     CDS             complement(1271..3430)
                     /gene="mobQ"
                     /locus_tag="FJMB_RS25850"
                     /old_locus_tag="FJMB80154_p420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_016245161.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLPPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     gene            3609..3863
                     /gene="mobC"
                     /locus_tag="FJMB_RS25855"
                     /old_locus_tag="FJMB80154_p430"
     CDS             3609..3863
                     /gene="mobC"
                     /locus_tag="FJMB_RS25855"
                     /old_locus_tag="FJMB80154_p430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091747.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     Typing          complement(3073..3431)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.056"
                     /coverage="100.0"
                     /evalue="3.94e-177"
     Typing          3432..3608
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          322..1013
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.821"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1275..3517)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.332"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tattggtttt ttgttttcat ccccccaagt tatccacagg ctcgccgtta agtttttaga
       61 tcttgttttt aagatcttgt ttttaatact tgttttaggg gtgaaattca ttaataaaat
      121 caatatgtta tagtggtata tgtggaggaa tttaaggtaa aggtggagga atttaaggtg
      181 tttaggtgga ggaatttaag gtaaaggtgg aggaatttaa ggtgttattc acagaaatgt
      241 gaggatataa aaaaaaacat tccacatttc ttatttgtgg ggcacattaa agcatcacca
      301 catttgaacg ggatctttgt atgtcggact tgattgccta caagtcgaat gctttggttg
      361 aggccagcta caagttgacc ctacaggaac aacgttttct gttgctgtgt atcagtcgat
      421 tgaaatctgg tgctgatgct gaattgcaaa agacaatgac cattaccgct gcggaatatt
      481 ttgattcatt ccctgacatg gggcggaaaa atgccgaggt tcagttacag gaagcgatcg
      541 atcgtttatg ggacaggtcg atcattctca agaatgacga gaagcgtgag gagttccgct
      601 ggatccagta tcgggcacag tacgctaaag gtgaggccaa ggctcgaata accttttctg
      661 acgcggtaat gccttatctg acgcaactga aggggcaatt cacgcgcgtt gttataaaaa
      721 acatatcagg tttatcaagt agttacagta taagaatata cgagttcctc cagcaattcc
      781 gcagtacggg agagagaatt attgctctta atgactttcg ttcaatgtta gggattgaaa
      841 ataaatacaa acaattcaga gacttaaata aaatactaat aaaaccctgt gttgacgaac
      901 tgaacaaaaa aagcgacctg gctgtaacgg ttgagacgat caaaaagggg cgcacggttg
      961 tagccctgca ttttcgcttc aaggaagata aacagatcaa gatgacgatc taaaatcgat
     1021 tttaaggcac ctaaaacgct ccctgctgcg ttttttttgt gttagtagtg cgaaggtact
     1081 attgaagtga aaaattgcgt caaaaacgct ctgatgcagc gaaaaaagaa cccccaattt
     1141 ttgggggcgg aaaaacaaaa ctatgcttgc attatgtgag tgtgcttctt gcattcatcc
     1201 ctgtcgatga acatcgaaga acatcgaaga acataacacg atgttaaaca aaacaaaata
     1261 ttgttcacat tcactgtgac caaccgcgat catgtctcct ctcggtggtg tgtgttcttg
     1321 catcttcctc gcgccgccat tcgtcccagg tacgattgaa acccagcgag cggaacagat
     1381 gatccggctg tttttctggt gtgttgacgg cagcaaaaag cggtgcctca cgggttttca
     1441 gatgaaactc cataagttcc ggcgcaggtg atatgtggct gtctgcatcg tgatttacgg
     1501 caacaccata ccctgcatac cgtaacccct tatcggtcag tgcactgatg accagctggc
     1561 tgttatcggc tgagggaacg gcaaacgcgg gatacagcgt accattaagc cggacaacac
     1621 cgcccagaga gtcatccacg cccatgacca gcaaaagctg aagctggcgg gtttcccggt
     1681 cgacagaact cattaccggc atcagcgtcg gtggcagacg tccctgcgta aaatccacct
     1741 tccttgctgc cagtgatggc atggctggct ggcggtcttc tccaagacag aaagactccg
     1801 gccatgtctt cggctggcgg agattgcggg tatattgcgg ccatgtctgg caaacctgcg
     1861 tcagaagttg ctcaaaggtc gcagaggaat aaacacgttg cagtggtgga tgttgaccga
     1921 tttcgcttcg gtcacagata taaccactcc tgtcacgcat taaatcaaca cgctcaaaga
     1981 ttctggtgcg gtatttctcc agctcgttcc cccagagcgt gtattctccg ttccggttgt
     2041 gaagcagaag gtaacaggtc atttccccgt taccaggcat gggggcctcg ccataccccg
     2101 taacgactgc gctcagtacc cctgaatcca taacggcatt gtcatttttc ctgagctgtt
     2161 ccgctcgtcg ctccgcgcga ctacgttctg tatcctcctg ctccttccgg cgcaattcct
     2221 cttcacgttt aagcaactgg actctgagag ctgcaaggcc ggatgccccc cactgctgcc
     2281 actgtttttc aaatgccgtc tgactcgcgg gaatgtcctg tttctccacc ttccggtaat
     2341 gctgtcgcaa tttttccatt tgctggctca gcgactggat ttttccctgt tcgctacggt
     2401 attccggtgc cgcccattgc atcaggggaa tacgggaaaa accggacgaa aacggcgatg
     2461 attttttcag gctggcagca tggctcatcg cttcctttcg ttcggtgttc agctcttccc
     2521 agcgtctgcg cgtcgcctgc atatctgcca tcatcgacat ggcattactg gtgagtttcc
     2581 atgccgcatt actgtccttc ggcaacgctt cgatgatgtc acgtagtgtg tacgaagttg
     2641 cgttgtcagg aagcacgata ttcagaaggt cagccacacg gcgggcaggc tctgtcagcc
     2701 cgctgttcct gagttcagtg cgggaaatct gtatttccag gttaagcctg ttaatcagac
     2761 gattctgttc gccgcgttct gtttcgatac cgcgtgtttc catcgcacag gcagactttc
     2821 cgaggtgaat accaggcgtt ttctcaagcc cctgtcgctc gtatgaacga tggtctatct
     2881 cttcctgata accggcgttc gccagtgctc tgtttgcatg gtcagcccat gatgcacgcc
     2941 acgtctcggc atgtgtccgg tcgttccagt ccctgacctt tccgccgaac ccagcaggtc
     3001 cgacagccct cgtggtcagc atgatgtgcg catggggatt tgttttatcc atgtgatgga
     3061 acgccacatc agcaatcatg ccctgactga caaagttttc tctgacaaag gcgagaacgg
     3121 tttcccgtgc ggcatcctgc ggcagttcac gggggatagc gagttcaatt tcccgcgcaa
     3181 gttgtgagtt cttccggcgt tcggccttct caacggcatt ccagagttcg gcacggttcg
     3241 tacaccaggc aggggctgtc gcgggggtca gaatcacagc ttcagccaca ccgcttttgc
     3301 gggtgtagtc gtgagtcagt ccggtacgct catcaagcat tagtgaaccg gaacgatagg
     3361 cagcggaagc tacggcgctg tagccgctgc ttctggagat aattttcatg ctgagatgat
     3421 aaatcgccat ttctgccgta atgccttttt gtagttttct ttcagccccg cagggcgcac
     3481 acaatcgaaa cggagtggag attgtataag tgcgcacttc gtgatttgcc gtgcattatg
     3541 ccagctcagt ttttaaacta ccagtgacgc gctaaaatgc gatttttcat cgcgcacgga
     3601 gttacatcat gagcagaacg ctcgaacaga agattgctga tgctgaggcg agattacagc
     3661 gcctgaaggc gaagagccgg agcctggaca cagcgcagaa ggttgttgtg ggtgcagcat
     3721 tgctggcaaa ggtcagaaag ccggaggagg tgcagttgcg tgcctggtta ctccagttcc
     3781 tgaaggcaga ggtgacacga caggctgatg tgacgcgcat actgccgctg attaacgagc
     3841 tggaggcgct gccaggacag tgattgtatc gtccgctcgc cgcagcccca gcgaccgagc
     3901 gttagcgagc gagtgggcga ggaagcggaa gtgaatccgt cgccaggttg agcacatacg
     3961 cacggttttt cggcaggtga tatgcgctga acccgcatga atactggatt tccacagggt
     4021 tggaagggga gtattcggaa cttgcaattc tgctttcttg caacacattg tttt
//
