LOCUS       NZ_AP024509             3166 bp    DNA     circular CON 14-MAY-2024
DEFINITION  Bacillus cereus strain HT18 plasmid pHT5, complete sequence.
ACCESSION   NZ_AP024509
VERSION     NZ_AP024509.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00278679
            Sequence Read Archive: DRR276629, DRR276630
            Assembly: GCF_019704155.2
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Matsunaka,M., Thanh,N.C., Uedoi,T., Iida,T., Fujino,Y., Ohmori,T.,
            Hiromasa,Y., Ohshima,T. and Doi,K.
  TITLE     Complete Genome Sequence of Bacillus cereus Strain HT18, Isolated
            from Forest Soil
  JOURNAL   Microbiol Resour Announc 11 (3), e0110621 (2022)
   PUBMED   35254113
  REMARK    DOI:10.1128/mra.01106-21
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Doi,K. and Matsunaka,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2021) Contact:Katsumi Doi Kyushu University,
            Microbial genetic resource laboratory; Motooka 744, Fukuoka, Fukuoka
            819-0395, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicyler v. 0.4.8
            Genome Coverage                   :: 80x
            Sequencing Technology             :: illumina Miseq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019704155.2-RS_2024_05_14
            Annotation Date                   :: 05/14/2024 04:22:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,848
            CDSs (total)                      :: 5,695
            Genes (coding)                    :: 5,540
            CDSs (with protein)               :: 5,540
            Genes (RNA)                       :: 153
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 106
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 58 of 155
            Pseudo Genes (incomplete)         :: 110 of 155
            Pseudo Genes (internal stop)      :: 39 of 155
            Pseudo Genes (multiple problems)  :: 43 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP024509.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="HT18"
                     /isolation_source="soil"
                     /db_xref="taxon:1396"
                     /plasmid="pHT5"
                     /geo_loc_name="Japan:Wakayama, Hashimoto"
                     /collection_date="2018-11-19"
     gene            complement(join(2249..3166,1..327))
                     /gene="mobV"
                     /locus_tag="WHT_RS29095"
     CDS             complement(join(2249..3166,1..327))
                     /gene="mobV"
                     /locus_tag="WHT_RS29095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017562244.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000951644.1"
                     /translation="MAHVQKYAKGNVQGLSIHWDRKTENHSNQDIDNERSHLNYDLCEK
                     EGDTLSRMNDRLSEVHCLNRKDVKVCADWAVTLPESLKDASEKEQREFFEKTYEFLANR
                     YGGEKNVLSANVHNDETRPHMHFAFMPVVWDEKKQQAKVSAKEVLTRKDLKTFHQDLDE
                     FLKKEIPQIYKEGILNDKTIGVDTVKDLKRYSEEIQKQKDVMDAEVKVKERELEGKIQS
                     LDKELESKKNEFLNLSEALPSKINIKVKGKEKKTEVVKTGFLKSETVTTETGNWIVSDS
                     EMRRMQKVLSDANFVKEDYERLQRTDLVKENKELHDRVDSLADGYVKAINENTDLYEKN
                     RELRKEISSLKAHVKDLKENVKVLYHNTKKVLGEHFKAFRGLVKNELDIKGVDNQFDCE
                     YKKEIKKQRGYNMER"
                     /name="mobV"
     gene            complement(695..928)
                     /locus_tag="WHT_RS29100"
                     /old_locus_tag="WHT_58730"
     CDS             complement(695..928)
                     /locus_tag="WHT_RS29100"
                     /old_locus_tag="WHT_58730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="WP_046199669.1"
                     /translation="MTKLEELEKDFNQMNLDLKAIQHDMKSLEVRILVAEKDVLTINKQ
                     LDKISANTTWILRLIISGLLTGVLGVVAKNLL"
                     /name="hemolysin XhlA family protein"
     gene            1735..2235
                     /locus_tag="WHT_RS29105"
                     /old_locus_tag="WHT_58750"
     CDS             1735..2235
                     /locus_tag="WHT_RS29105"
                     /old_locus_tag="WHT_58750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182216.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_001182216.1"
                     /translation="MQPERPLKKVSKVTKNKIIDINTGEIQEEEEIINAYIDKEPDYVK
                     IYLKDIVALNDLPKGLDRVINVLLKVMSYDNMIILNSFIKKQMAQELGYKTVQVLNNNI
                     NKLVNAEILFRKGTGTYQVNARYFGRGHWHDIQEIRLQQVYSTKGRELKTEITYKNDEP
                     SQN"
                     /name="replication/maintenance protein RepL"
     Typing          complement(2..327)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.6400000000000004e-66"
ORIGIN
        1 tttttcacca ccataacggt tcgctaaaaa ttcataggtt ttctcaaaaa attcacgttg
       61 ttccttctca gaagcatctt tcaaactttc cggtaatgta acagcccaat ctgcacagac
      121 ttttacatct tttcgattca agcaatgcac ttcacttaac cgatcattca tgcgtgaaag
      181 tgtatctcct tctttttcac ataaatcata attcaaatgt gaccgttcat tatcaatgtc
      241 ttgattcgaa tgattttctg tttttctgtc ccagtggatg gacaatcctt gaacatttcc
      301 ttttgcatat ttttgaacgt gagccatgag caagaccccc ccttttggta actagtttaa
      361 catggggata gaagaaaaac caaatttggt ctagcacgtt ataccaaatt tcatttggac
      421 gtgctctgcg aggctctttg gctccgccaa gccatgcttt gcctggcaaa aaacaaacct
      481 tgggctttgc ccaaacccac tggggaaact cttccccaga ccccctcaat caaactcccc
      541 aacccctcaa tccttgaggg gctccctcgc cggttagcag gaacaaacga ttgtttgttc
      601 aatgccaaga gggtttgggt gtagtacggt tggtcaagtc aaatactccc ttcgctttgc
      661 taggtccgtt ttgattgacc aacacccttt tttcctataa caggttttta gctactacac
      721 caagtactcc tgttaacaat ccgcttatga tcaatcgtaa aatccatgta gtatttgcac
      781 taattttatc taattgttta ttaatagtta aaacatcctt ttcagctact aatattctta
      841 cttctaaact tttcatatca tgttgtatag cttttaaatc tagattcatt tgattaaaat
      901 ctttttctag ttcctccaat tttgtcatat aacatacttc tccttttaaa ttaattttaa
      961 atatattata tgtatatggt aattagagaa taatgatata aaaagagaac tcaaatctat
     1021 tattaagatt taagttctct ttaaagataa gggaattata cgaaagcgta atatagcata
     1081 tgcttatcct ttttaatatg catctagcaa aaacacagtt tctataaatg tgctgttcta
     1141 agttcattct ctccttgttt ttgggtgtga gtgctggctg ggggtttggg ggctagcccc
     1201 ctggatctta acgtaaatga atatggaata agcggattgt ttagaacgat agagcaaggc
     1261 ggttcggcag gtacaattgc tagtccaagt cgaacaatct aaatcctcgt ttttggtctg
     1321 ttcggcttgg cgacatgctc acacctgccg aatttcacta tgcaatagca aaagagtgga
     1381 gattttttga atggttttct caaaaaatac tactcgacta ttgcatagtg aaaagccctt
     1441 ttaaatttta taaaataata gaatagaagg gagaacatag gttgaaccgt tgatatgact
     1501 ggatttctag gaaaatacta taatttgtgg gattataagt tataatttgt gggattataa
     1561 ccgtaatttg tggagttata taccgtaatt tgtggagtta taaattaaat ttatttttta
     1621 tatcatttat aatttgtggg attataaatg tgaaatccta taaaaaatat tgcactttta
     1681 aaatacccga tttataattt aattaaatta taaatcgggt aggaggtaga tttgatgcaa
     1741 ccggaaagac ccttgaaaaa agtttctaaa gttacaaaaa ataaaataat agatataaat
     1801 actggtgaaa ttcaagaaga agaagaaatt ataaatgctt atatcgacaa agaaccagac
     1861 tacgtaaaaa tatatttaaa agatattgta gccttaaatg atttacctaa aggtttagat
     1921 agagttatta acgttttatt aaaagtaatg tcttatgaca atatgatcat tctaaatagc
     1981 tttataaaaa aacaaatggc acaagaacta ggatataaaa cagtacaagt tcttaataac
     2041 aacatcaaca aacttgtaaa tgcagaaatt ctttttagaa aaggtactgg tacatatcaa
     2101 gtaaatgctc gttattttgg tcgtggccat tggcacgata tacaagaaat ccgattacaa
     2161 caagtctatt caactaaagg aagagagctt aaaacagaaa taacatacaa aaacgacgaa
     2221 ccgtctcaga attaattctg agacggtttt aacgttccat gttatatcca cgttgtttct
     2281 taatttcttt cttatattca caatcaaatt gattatctac accttttata tccaattcat
     2341 tcttaacaag tcctctaaac gctttaaaat gctctccaag gacttttttc gtattgtggt
     2401 ataaaacctt tacattttcc tttaaatctt ttacatgggc ttttaacgag cttatttcct
     2461 tacgtaattc tctattcttc tcatacaaat ctgtattctc attaatagct ttaacataac
     2521 catcggctaa actatcaact ctatcatgta attctttgtt ttctttaact aaatctgtac
     2581 gctgcaaacg ctcataatct tcctttacga aatttgcatc gcttagaact ttttgcatcc
     2641 ttctcatttc actatcagaa acaatccaat tcccagtttc tgtagttact gtttctgatt
     2701 ttaaaaaacc tgtttttaca acctctgttt ttttctcttt tccttttact ttgatattaa
     2761 tttttgaagg gagtgcttca ctcaaattca gaaactcatt ttttttactt tccaattctt
     2821 tatctaatga ttgaattttc ccctctaact ctctttcttt gacctttact tccgcatcca
     2881 ttacatcttt ttgtttctgt atttcttcag aatacctttt caaatcttta accgtatcaa
     2941 cgccaatcgt tttatcgttt aaaatacctt ctttgtagat ttgtggtatt tcttttttca
     3001 aaaactcatc taaatcttgg tgaaatgttt ttaaatcttt tcttgttaaa acttctttag
     3061 ctgaaacctt tgcctgttgc tttttctcgt cccaaacaac aggcataaaa gcaaaatgca
     3121 tatgcggtct tgtttcgtca ttatgtacat tggctgacaa cacatt
//
