LOCUS       NZ_AP024507             9190 bp    DNA     circular CON 14-MAY-2024
DEFINITION  Bacillus cereus strain HT18 plasmid pHT3, complete sequence.
ACCESSION   NZ_AP024507
VERSION     NZ_AP024507.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00278679
            Sequence Read Archive: DRR276629, DRR276630
            Assembly: GCF_019704155.2
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Matsunaka,M., Thanh,N.C., Uedoi,T., Iida,T., Fujino,Y., Ohmori,T.,
            Hiromasa,Y., Ohshima,T. and Doi,K.
  TITLE     Complete Genome Sequence of Bacillus cereus Strain HT18, Isolated
            from Forest Soil
  JOURNAL   Microbiol Resour Announc 11 (3), e0110621 (2022)
   PUBMED   35254113
  REMARK    DOI:10.1128/mra.01106-21
REFERENCE   2  (bases 1 to 9190)
  AUTHORS   Doi,K. and Matsunaka,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2021) Contact:Katsumi Doi Kyushu University,
            Microbial genetic resource laboratory; Motooka 744, Fukuoka, Fukuoka
            819-0395, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicyler v. 0.4.8
            Genome Coverage                   :: 80x
            Sequencing Technology             :: illumina Miseq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019704155.2-RS_2024_05_14
            Annotation Date                   :: 05/14/2024 04:22:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,848
            CDSs (total)                      :: 5,695
            Genes (coding)                    :: 5,540
            CDSs (with protein)               :: 5,540
            Genes (RNA)                       :: 153
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 106
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 58 of 155
            Pseudo Genes (incomplete)         :: 110 of 155
            Pseudo Genes (internal stop)      :: 39 of 155
            Pseudo Genes (multiple problems)  :: 43 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP024507.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9190
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="HT18"
                     /isolation_source="soil"
                     /db_xref="taxon:1396"
                     /plasmid="pHT3"
                     /geo_loc_name="Japan:Wakayama, Hashimoto"
                     /collection_date="2018-11-19"
     gene            complement(join(8109..9190,1..124))
                     /gene="mobV"
                     /locus_tag="WHT_RS29000"
     CDS             complement(join(8109..9190,1..124))
                     /gene="mobV"
                     /locus_tag="WHT_RS29000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033080.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_050267888.1"
                     /translation="MSRFMVCHMKKFKMTDVKGLQIHNQREKESHSNPDIIQERTEQNY
                     DLIHGKENVDYKKLIQDRIQDGVVSDRAIRKDAVVCCSFMISASPDYMNSLSQAEQRRF
                     FEESVCFLKARYGEENFVYASVHVDEKTPHMHVGMVPVNEKQKLSAYSFFKNKSELHDL
                     QDKIYEHVKEKGFDIERGVSSDRKHLSAQRFKAVTLQQEIEKLEQEKNEIDSRLHDLKL
                     SLDKAKSVDEIPVKEKGGFIRSKTVEIALEDFESIKVLAKSSETLREENKRLKNEKVKS
                     EYEKDNLYKEQRFLERKVTDLKRENEGLKGENDFLKKTLDRVKDLYKEKLPEFAGMIGY
                     VKASILDKMNRKFLKRHFAGDDEVSGAQKFLNHKQEHEEQQKRLKQVRRSQQKNRDQGL
                     ER"
                     /name="mobV"
     gene            complement(755..1423)
                     /locus_tag="WHT_RS29005"
                     /old_locus_tag="WHT_58560"
     CDS             complement(755..1423)
                     /locus_tag="WHT_RS29005"
                     /old_locus_tag="WHT_58560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002081870.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="WP_215253610.1"
                     /translation="MRYSDIKVGNIYYADLNPIRKYEFGDNHLSIILSKGKDKRTVTIV
                     SLTSKSSGLGQNKMNLGIVSGLPKRLVEDRSGNPINTYVVLDQVRTVSANRIQYIKDGK
                     KTDGTDNYIECPVDAFSFSKIVCELADLRIADLNDEDAIGEYHKKTFFNYCVKKMIDLT
                     YDIIKGRGIVADKKEEVIYFYNNALAMEKGFLIDNYLKPHDIKNKVLEKFNEIVLMSVK
                     "
                     /name="type II toxin-antitoxin system PemK/MazF family
                     toxin"
     gene            complement(1998..2225)
                     /locus_tag="WHT_RS29010"
                     /old_locus_tag="WHT_58570"
     CDS             complement(1998..2225)
                     /locus_tag="WHT_RS29010"
                     /old_locus_tag="WHT_58570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869766.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_000780193.1"
                     /translation="MKLVNNIRMIMAQKNIDNIAELIRITGVSRNSINKLWHNESVSSL
                     RLDTLIAICEKLDVKLSDLIEYIPGDIESK"
                     /name="helix-turn-helix transcriptional regulator"
     gene            2414..2701
                     /locus_tag="WHT_RS29015"
                     /old_locus_tag="WHT_58580"
     CDS             2414..2701
                     /locus_tag="WHT_RS29015"
                     /old_locus_tag="WHT_58580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000323835.1"
                     /translation="MAYLTAKKVKGNIYFYVSQYVGTQPYYSKHYRYINIYSIGNQKIA
                     LERIAMWLLDNNRIPKELIEIGVSINDVKYWYEKVEKTLQNYSLTNHKNT"
                     /name="hypothetical protein"
     gene            2715..2978
                     /locus_tag="WHT_RS29020"
                     /old_locus_tag="WHT_58590"
     CDS             2715..2978
                     /locus_tag="WHT_RS29020"
                     /old_locus_tag="WHT_58590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000874968.1"
                     /translation="MAFLTNYKANGKRYFYVEKYVGKKPYTCKQSERIYSIGNERITLE
                     RLTLWILDNSFIPSELIKIGISIDDIENWREKVENTIKRYSL"
                     /name="hypothetical protein"
     gene            complement(3680..3886)
                     /locus_tag="WHT_RS29025"
                     /old_locus_tag="WHT_58600"
     CDS             complement(3680..3886)
                     /locus_tag="WHT_RS29025"
                     /old_locus_tag="WHT_58600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000947848.1"
                     /translation="MLSEQVIYYWNDELQYYYLAEKKNGSYKFVQPLKKLELDDISDPL
                     EREQMKKMLEIKKRMSETKGNWF"
                     /name="hypothetical protein"
     gene            complement(4334..5713)
                     /locus_tag="WHT_RS29030"
                     /old_locus_tag="WHT_58610"
     CDS             complement(4334..5713)
                     /locus_tag="WHT_RS29030"
                     /old_locus_tag="WHT_58610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000650.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_050279502.1"
                     /translation="MNDVLKSNLLDITIDKLSIVADFKEGIKKEEFIRMVTSSNMPYSL
                     QANRSKNFGYEEVLKCDSVDCGYIELAGELKKASVDLPKLISHRIRLEIQIEQEKKARE
                     VGESLLSNAEYQGLFESLADVKELIAETDEDGRLTDERKLKRHERQIELTIEKLKGEIV
                     ALKSEIDEQELATGFKSEAMEKIHEEHVQILLNTLENKEKLIEVKRDELLRNAEMFLQL
                     DANGFLPNNGKKKNDRLLKDVRFEFNPKHMAYNRVVDEAIRLVLGLLENIEVTGIHIAC
                     DYPVKIADLKIKDLSSKSECIYLDRDKKIETMYIGKRGSDNHLCIYNKKRENEENETLD
                     QYPDVEHVTRFEARLRKKKAKEWIDSEYNPFDSIIVGDLEKVDNSKIKVNDRIVLEAIM
                     NDYHGRYFGMMDKDQKSNWRKKIKQHVPKLFDVAGDYEKKKVLLTGELAELMKNKPSKQ
                     K"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            complement(5875..6081)
                     /locus_tag="WHT_RS29035"
                     /old_locus_tag="WHT_58620"
     CDS             complement(5875..6081)
                     /locus_tag="WHT_RS29035"
                     /old_locus_tag="WHT_58620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000030440.1"
                     /translation="MSKANYVTVGRKPKDGKNPKIPETFRLPKETSERIKGLVSSGRFS
                     TKAEIAEKAFEFYFSYLEKENGK"
                     /name="hypothetical protein"
     gene            complement(6205..6468)
                     /locus_tag="WHT_RS29040"
                     /old_locus_tag="WHT_58630"
     CDS             complement(6205..6468)
                     /locus_tag="WHT_RS29040"
                     /old_locus_tag="WHT_58630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000085833.1"
                     /translation="MSRERVKIKFKSEDIKAEIEGYSDMPYWNHDLKLEFILRLLNTVD
                     EKQFLESQNHDQLDYYTKEMIKMIEASLGFDGLYRLVYGDEL"
                     /name="hypothetical protein"
     gene            complement(6700..7461)
                     /locus_tag="WHT_RS29045"
                     /old_locus_tag="WHT_58640"
     CDS             complement(6700..7461)
                     /locus_tag="WHT_RS29045"
                     /old_locus_tag="WHT_58640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000957234.1"
                     /translation="MLTNTFLSKVYFALDYSDFFEIDDFSVTLPEEKSYSQELIIKYLF
                     DETCYFHTYINGSSEFRYECSPGKVMKIQKGNVSGVDIYTTEIRDWVKRMEDEFKTTPL
                     ARRVMKNQSQVEELIDKVDSYFVENGLDSNDKFSHPEMEEMESKLEDFKALIEERLAEQ
                     ITEKDFLKQEVSKLFKEIDFLKKQVQILTKKNWFKAFCSKTGKFIRNNPETTKAIAHTA
                     KECLPESIKMSIPEGATEVMNTLLEEQKVEN"
                     /name="hypothetical protein"
     gene            complement(7796..8077)
                     /locus_tag="WHT_RS29050"
                     /old_locus_tag="WHT_58650"
     CDS             complement(7796..8077)
                     /locus_tag="WHT_RS29050"
                     /old_locus_tag="WHT_58650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002026684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_065382350.1"
                     /translation="MEYFHSFGVNFINTVKDLAEKNNLTIEETCRILELTMKIDDYDRK
                     DEQLAGIGELIKGMNEQIMGLGEGLSDISKSLSDMSESIALVSLDDKE"
                     /name="hypothetical protein"
     Typing          complement(4366..5704)
                     /name="rep_cluster_268"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.469"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          complement(8113..9188)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.136"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ccgttcgttc ttgaataata tctggatttg aatgactttc tttttcacgt tgattatgta
       61 tttgtaatcc tttcacatct gtcatcttga attttttcat atgacaaacc atgaatcttg
      121 acatgggctt cgctcctttt tcggtggtag ttccatgtaa actgtagcac actacagttt
      181 actgcgtaaa cacgtgtgcc ctacggggca tttcggctac caccgagcca tgacaatcat
      241 ggcaaaaaac catgggcttt gcccaaaccc acaagggaaa aacttcccct tgacccctta
      301 caatcgaact ccccaacccc tcaatccttg aggggctccc tcgccggtag gcatccccaa
      361 acgttcggtt tgggaaaacc aagagggttt tgttgtagta aagatgtcaa ctccttatcc
      421 tcccttcggt ccggtaccgt tgacatcccc cacacaaagt tcatatctct ttaaaagtaa
      481 tccccaataa aaaaagcccc cctttatttt agagttaaaa ccctcaaaac aaaggaagcc
      541 atcattttca taaataacaa tcaaaaagac ctttcttttt acagaaaggt ctctctatgg
      601 ttgagccatc acagctcgcc aactattgtt cgggtcatca caaccctaaa ttctacggtt
      661 gagccatcac agctctttcg caattagtat taactaacgc tacttcttct aaccacactg
      721 tatcaaaaat aataataaaa atcaacactt ataattattt tactgacatt aaaacaatct
      781 cattgaattt ttccaacacc ttatttttta tatcatgtgg ttttaaataa ttatcaatta
      841 aaaaaccttt ttccatcgct aacgcattgt tataaaagta tattacttct tcttttttat
      901 ccgcgacgat ccctcgtccc ttaataatat cgtatgttaa atcaatcatt ttctttacac
      961 aataattaaa aaaagttttc ttatgatatt caccaattgc gtcttcgtca ttcaaatcag
     1021 caatccttag atctgccaat tcacatacaa ttttagaaaa agaaaacgca tcaactggac
     1081 attcaatata attatcagta ccatcagttt ttttgccatc ttttatatat tgaatacgat
     1141 tagcggacac tgtacgaact tgatccaaaa caacatatgt attgatcgga ttcccacttc
     1201 gatcttcaac taaacgttta ggcaatcccg atactatacc taaattcatt ttattttgtc
     1261 ccaaaccaga acttttcgaa gtaagagaaa caatagtcac tgtcctttta tctttacctt
     1321 tacttaaaat tatacttaaa tgattatctc caaactcata tttccttatt ggatttaaat
     1381 ccgcataata tatattcccc acttttatat ctgaatacct caacaatagt tccccctata
     1441 attaattact tattcacaag tttttacctt taaaacataa acaattggac tatatacagc
     1501 tttcaatcct catcgctaca cacttgtcca ttacccttgt acaatatagt aacttctctt
     1561 ttttttggta aatttgatca cctacaaata gcttttaacc atttttttta aatctcacat
     1621 accaagttaa atttagctta acataacaat aaacaattcg cttttcaaaa aatgctctat
     1681 cttgttcaat aaacaaaaaa cataaaaaaa agccctgaaa ataggactct ttttaataat
     1741 catcattacc accatttcat ttaacagcac tactatgatt ataaatatag ttcaacaaaa
     1801 tcacgaagta tgccagtacc tctcatcttc caaggtgttg acgcacctag caagtctaca
     1861 attccatatc cctaattcaa aacatactca tgcgttaaca aaagaagttg aagtgcgcca
     1921 tatagcacca caaaatacca ttagccaact atacctttag catacaaaaa ctatccaagt
     1981 acataaaatc ccctaaatta ttttgactca atgtcacctg gaatatattc aattaagtcc
     2041 gataatttaa catctaactt ctcacagatc gctattaaag tatctaatct taaagacgac
     2101 acactttcat tatgccaaag cttattaata gaattacgac tcacaccagt tatccttatt
     2161 aactccgcaa tattatctat atttttttgg gccataatca tacgaatgtt atttactaac
     2221 ttcattcttc ttactcctta tattcaacat acactctatt ttacttataa agataatttt
     2281 atgcaattta taagtaaaaa taaacctttt aaaggttata tttatttata tgtattgata
     2341 ttttaacttt ataaggttaa aatataacca cagacaaaaa caaaagaata atgaacatga
     2401 aaggaagaac gatttggcat atctaacagc aaaaaaagta aaaggaaaca tttactttta
     2461 tgtatctcaa tacgttggaa cacaaccata ttactcaaaa cactatagat atataaatat
     2521 ttactcaatc ggcaatcaaa aaatagcttt agagagaatc gctatgtggt tattagataa
     2581 caaccgcata ccaaaagaac taatagaaat cggagtatcc ataaacgatg tgaaatattg
     2641 gtatgaaaaa gttgaaaaaa cattacagaa ctactcttta actaaccata aaaataccta
     2701 aaaggagcat tactatggca tttctaacaa attacaaagc aaatggaaaa agatactttt
     2761 atgtagaaaa atatgttggt aaaaaaccat atacctgcaa acaaagtgaa cgtatttatt
     2821 cgattggaaa cgaaagaatc acattagaaa ggctcacttt atggatttta gataacagtt
     2881 tcattccaag cgaattaata aagatcggaa tatcaattga cgatatagaa aactggagag
     2941 aaaaagtaga aaataccata aaaagatact ctctataact aaaaacaaga aaaaagactg
     3001 atatttttat cagtcttttt tcctttaact atttatattt tttcaccaca aaagggttag
     3061 atattttaat aatatattta tatatttaat aaaagttatg cgtgactgtt tgtttttttc
     3121 atttgcgaac aaaaacaaac agactttaag ggtaaaaagg attaaaaata tacagaaatc
     3181 acgaaataaa aaattcacaa aacaacaaat tcaaacataa tctaaacatc tttttttatc
     3241 acacaactcg attaaaacgg tgcgactttt taatgatata tatatatcta tgaaatgaaa
     3301 atgcgagcaa agttttttcg ttttcagttc atcatagaga tactatataa actccgtaat
     3361 ttcggaatac ggaattacaa agaaaacaga aaacaaaaat ttcgaaaatc gaaaaatcga
     3421 gatttcaaat tcaacaaaat tcaaaatcaa attccacatc aaaatctccc ccaaaaaata
     3481 aaccttcaaa aaacgtacgg tattttatcg ttatatatat aactaaaaaa attcatgcgc
     3541 acactttagt tttccatcct gtacggtagt aacgggaagt tttaggatat aaactatata
     3601 gcttataaat ttaaaaaaga attcacaaat tcacgaaatt aaagttttaa aaaaagaaag
     3661 acattttgtc tttctttttt taaaaccaat taccttttgt ttctgacatc cgttttttta
     3721 tctctagcat ttttttcatt tgttctcttt ctaacggatc actaatatca tctaattcta
     3781 atttttttaa cggttgtaca aatttatagc tgccattctt tttttcagct aaataataat
     3841 actgtaattc atcattccaa taatatatga cttgttcact caacataaaa gcccccagta
     3901 atcaccttaa tattagccca ttacaccatc atagcatcaa tgaagcccta aaattaacgc
     3961 aatacaaaag agacagttcc aaaagtaacc gtatcaaaaa gtagtctcaa ataactgata
     4021 aacgcatcat aaagccaaaa acaagtgtct caaaaagtcc ctaaaatcat ttgcaaacgt
     4081 tcataaaaat atatataatt tatgaacggt agggttatat gttttatata tataaaaaac
     4141 cgcacccttt ttgggttatt tttctttgat gacaatcaat ataaaagtca ttaaaatgaa
     4201 taatatacgg tatacatgca ataatagacg tagttattct caaaactaaa tttaagttct
     4261 aaaccataaa aaaatccttc caagtgcgcc aacactcgaa aggattgtaa gtatatcttt
     4321 ttaaattcaa cttttatttt tgcttactag gtttattttt catgagttct gctaattctc
     4381 cagttagtag aacttttttc ttttcataat ccccagcaac atcaaacaac ttaggaacat
     4441 gttgttttat tttctttctc caattactct tttgatcctt atccatcatc ccaaaataac
     4501 gaccatgata atcattcatg atagcctcta aaacaatacg atcgttcacc ttaattttac
     4561 tgttatctac tttttctaaa tctccaacaa ttatcgaatc aaacggatta tattccgaat
     4621 ctatccattc tttcgctttc ttcttacgca aacgagcttc aaaacgtgtt acatgttcaa
     4681 catcaggata ctgatctaaa gtttcatttt cttcattctc tcttttttta ttatagatac
     4741 aaagatgatt atccgaacct cgtttaccaa tgtacatcgt ttctattttt ttatctctat
     4801 ccaaataaat acattctgat ttactagaca aatcttttat ttttaaatct gcaattttaa
     4861 ctggataatc acaagctata tgtatacctg taacttcaat attttctaac aatcccaaca
     4921 ctaatcgtat cgcctcatca accacacgat tatatgccat atgcttagga ttaaattcaa
     4981 atctaacatc tttcaacaaa cgatcatttt tcttttttcc gttattcgga agaaaaccat
     5041 ttgcatctaa ctgtaaaaac atttcagcat ttcttaacaa ctcatctctt ttaacttcaa
     5101 tcaatttctc tttattttcc aaagtattaa gtagtatctg aacatgttcc tcatgaattt
     5161 tttccatggc ttcactttta aaaccagttg caagttcttg ctcatctatt tcagatttca
     5221 atgcgactat ttcgcctttc aacttctcaa tagtcagttc tatttgtctc tcgtgccgtt
     5281 tcaatttcct ttcatcagtt aaacggccat cttcatcagt ttcagctatc aattctttca
     5341 catcagctaa actttcaaac aaaccttgat actcagcatt agaaagtaac gattctccaa
     5401 cttctcttgc tttcttctcc tgctctattt gaatttctag tctaatccta tgagagatta
     5461 atttaggaag atcaactgac gcttttttta attctcccgc caactcaata taaccacaat
     5521 caacagagtc acactttaaa acttcctcat aaccgaaatt tttcgaacgg tttgcctgca
     5581 atgaatatgg catatttgaa cttgtaacca ttcttataaa ttcttctttt ttgatacctt
     5641 ctttaaaatc tgcaacaata cttaatttat caatcgttat atctaacaaa ttagatttta
     5701 gtacatcatt cataacaaaa tcctaaaccc ccaaacagtt atatttttga acccgtccaa
     5761 agcgtagttg gataataatc gagaaattct cgaactttac ctgcttatta gaataacagg
     5821 taactttttt cgagagttaa tctccctcaa ctaaaaaatc attccaaaac acatttactt
     5881 cccattttct ttttccaaat aagaaaaata aaactcaaat gctttttctg caatttccgc
     5941 tttcgtagaa aaacgtccac tactaaccaa tcccttaatc ctttcagatg tttcttttgg
     6001 caatctaaaa gtctcaggga ttttcggatt cttaccgtct ttcggtttcc taccaacagt
     6061 cacataatta gctttactca ttaaaacacc tccaaataac ccagtaacaa catgttatca
     6121 tttcgtatga cgttttgtaa ataactttgt tttaaaataa cataaaatcc gaatataaaa
     6181 gaactcataa agagttctcc caacctataa ttcatctcca taaaccaacc gatataatcc
     6241 atcaaaccct aaactagctt caatcatttt aatcatttct ttagtgtaat aatccaactg
     6301 atcgtgattc tgactttcaa gaaattgttt ttcatcaact gtatttaaaa gtctcaaaat
     6361 aaattccagt ttcaaatcat gattccaata aggcatatca gagtaacctt caatctccgc
     6421 ttttatatct tcagacttaa attttatttt cacccgttct ctagacatac ttatccctct
     6481 tttccctagt tataaatatt agaaacatca ataaaaaaac atcacagacc attgatacca
     6541 aagtattttc taataacatc acccacttcg cgccccgaga tgtttctaat atatcaagta
     6601 atataattaa tccttttctt taacaataaa aaaataggac tcttgcataa taaatataaa
     6661 caagagtcct attaccatta aatatttcta atgcaaattc taattttcaa ccttttgttc
     6721 ttccagcaaa gtgttcatta cttcagtagc cccttctgga atactcattt taatagattc
     6781 cggaagacat tctttagctg tatgggcaat cgcttttgta gtctcaggat tatttcttat
     6841 aaacttacca gttttactac aaaatgcttt aaaccaattc tttttagtta atatctgtac
     6901 ttgcttttta aggaaatcta tttccttaaa aagtttcgaa acttcttgtt ttaaaaaatc
     6961 tttttctgta atttgttcag ccaatctctc ttcaattaaa gctttaaaat cttctaattt
     7021 gctttccatt tcctccattt ccggatgaga aaacttgtca ttagaatcca aaccattctc
     7081 tacaaaataa gaatccactt tatctatcaa ttcttctact tgagattgat tcttcattac
     7141 tctccttgct aaaggtgtag ttttaaactc atcttccatt cgctttaccc aatcccgaat
     7201 ctcagtagta taaatatcta ccccagaaac attccccttc tgaatcttca taacctttcc
     7261 agggctgcac tcataacgaa attccgatga tccattaata taagtgtgaa aataacaagt
     7321 ttcatcaaac aaatacttta tgattaattc ttgagaataa cttttttctt ctggaagtgt
     7381 tactgaaaaa tcatcaattt caaagaaatc actatagtcc aacgcaaaat aaactttact
     7441 tagaaacgta ttagttaaca caaaaaatcc tcccaacttg tatataccca acaataaaaa
     7501 tacacaaaat aaaaacaata aagtcacaca agactttact atcctcatta aaattaaata
     7561 taccaatatc taacaacata atacacatct aaaaacactt atttcaattt ccttttttta
     7621 aaaacaagag ccatccccaa tccccttgta taatatcccc atatattcac atcaacaatt
     7681 tacgaggacg ttatacaagg ctctacaatc ctcgctatat ttcacaaaaa taagttccac
     7741 taatgctaac ttctaaagaa taaagggaac tcccatagag tcccccaaca cactattact
     7801 ctttatcatc caaagatact aacgcaatac tttcactcat atcactcaaa cttttactta
     7861 tatcactcaa gccttcacca agtcccataa tttgttcatt catccctttg ataagttctc
     7921 caataccagc taattgttca tccttacgat catagtcatc aattttcata gtcaattcca
     7981 aaatcctgca agtctcttca atcgttaaat tatttttctc agccaaatcc ttcacagtgt
     8041 ttataaagtt cactccaaag ctatgaaaat actccatcct tggttgttga ttaaataatg
     8101 tccccacact atctctccaa tccctggtcc cgatttttct gttggcttct tcgcacttgt
     8161 tttaatctct tttgttgttc ttcatgctct tgtttgtgat tcaagaactt ttgagcacca
     8221 cttacttcgt catcaccagc aaaatgtcgt tttaaaaact tgcgattcat cttatccagt
     8281 atactcgctt taacataacc aatcattcct gcaaattcag gcaacttctc cttatacaaa
     8341 tctttcactc gatccaacgt tttcttaaga aaatcatttt cgccctttaa cccttcattc
     8401 tctcttttta aatcagttac tttcctctct aaaaaacgtt gttctttata taaattatct
     8461 ttttcatatt cactcttaac cttttcgttt ttaaggcgct tattttcctc tctaagcgtt
     8521 tcagaagact tagccaatac ttttatactt tcaaagtctt ctaatgcaat ctcgaccgtt
     8581 ttagagcgta taaacccacc tttctcttta acagggatct catctacact tttagcttta
     8641 tctaaagata atttcaaatc atgaagacga ctatctatct cgttcttttc ctgttctaac
     8701 ttctctattt cttgttgcaa agtcaccgct ttaaagcgct gcgccgataa atgtttcctg
     8761 tcactagaaa caccacgctc tatatcaaat cccttttctt tcacatgttc atagatttta
     8821 tcttgtagat catgaagctc tgatttgttt ttaaaaaatg aatacgctga tagtttttgt
     8881 ttttcattaa ccggaaccat tcctacatgc atatgaggtg ttttttcatc aacatgcact
     8941 gaagcatata caaagttctc ttctccatac cttgccttta aaaaacaaac agactcttca
     9001 aagaatcgtc tctgttccgc ttgagataaa gaattcatat aatcaggact agccgaaatc
     9061 ataaacgaac aacaaacaac agcatctttc ctaatggctc tatcagatac aaccccgtct
     9121 tgaattctat cttgaattag ttttttataa tccacgttct ctttaccatg aatcaaatca
     9181 taattctgtt
//
