LOCUS       NZ_AP024506             9518 bp    DNA     circular CON 14-MAY-2024
DEFINITION  Bacillus cereus strain HT18 plasmid pHT2, complete sequence.
ACCESSION   NZ_AP024506
VERSION     NZ_AP024506.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00278679
            Sequence Read Archive: DRR276629, DRR276630
            Assembly: GCF_019704155.2
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Matsunaka,M., Thanh,N.C., Uedoi,T., Iida,T., Fujino,Y., Ohmori,T.,
            Hiromasa,Y., Ohshima,T. and Doi,K.
  TITLE     Complete Genome Sequence of Bacillus cereus Strain HT18, Isolated
            from Forest Soil
  JOURNAL   Microbiol Resour Announc 11 (3), e0110621 (2022)
   PUBMED   35254113
  REMARK    DOI:10.1128/mra.01106-21
REFERENCE   2  (bases 1 to 9518)
  AUTHORS   Doi,K. and Matsunaka,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2021) Contact:Katsumi Doi Kyushu University,
            Microbial genetic resource laboratory; Motooka 744, Fukuoka, Fukuoka
            819-0395, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicyler v. 0.4.8
            Genome Coverage                   :: 80x
            Sequencing Technology             :: illumina Miseq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019704155.2-RS_2024_05_14
            Annotation Date                   :: 05/14/2024 04:22:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,848
            CDSs (total)                      :: 5,695
            Genes (coding)                    :: 5,540
            CDSs (with protein)               :: 5,540
            Genes (RNA)                       :: 153
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 106
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 58 of 155
            Pseudo Genes (incomplete)         :: 110 of 155
            Pseudo Genes (internal stop)      :: 39 of 155
            Pseudo Genes (multiple problems)  :: 43 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP024506.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9518
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="HT18"
                     /isolation_source="soil"
                     /db_xref="taxon:1396"
                     /plasmid="pHT2"
                     /geo_loc_name="Japan:Wakayama, Hashimoto"
                     /collection_date="2018-11-19"
     gene            complement(join(9062..9518,1..197))
                     /locus_tag="WHT_RS28945"
                     /old_locus_tag="WHT_58550"
     CDS             complement(join(9062..9518,1..197))
                     /locus_tag="WHT_RS28945"
                     /old_locus_tag="WHT_58550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016136977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050267873.1"
                     /translation="MTKVVDFGQAEKKAKIRDSKIDSIYDQLQAGGYSEEEKAMLLQLL
                     SKTTGGDEYFIGKKKKPTDRVRFVQLIMDNVNYLTEIEYLSSKEEAFLFKLAPYVEFKT
                     NVIIQKIDKDNVDTTTPASPTYLAERFKMARKNVSLTMNGLFKKGVLGVAAAGITTEDG
                     RACTSRTWFVNPNIMCCSPKDGIDKATQHIFRNSLRNFKIDESKKKYKLPIYLF"
                     /name="hypothetical protein"
     gene            complement(407..1873)
                     /locus_tag="WHT_RS28950"
                     /old_locus_tag="WHT_58440"
     CDS             complement(407..1873)
                     /locus_tag="WHT_RS28950"
                     /old_locus_tag="WHT_58440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901551.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_050267870.1"
                     /translation="MLGSISFNQSHQSSLSHNNRENIHGNPGIDPARSEENIYFVQKDI
                     RSVYKDVFQEAVDKYNEKQKRNDRKIENYYDKIHKNDKTHEQRELVVAIGEGKDDPKYR
                     VAKKEALKRYAEAFQERNPNLAVYNMVLHDDEANPHLHINYVPNFESSRGLTRRVGMDR
                     ALQQQGVEGTGRKLIAHWRELETAYIEQLAKEHIPNFERANVGSHKYMKVRQYKEYAEA
                     KSTIENQVQEKETELQTIDHDLKNVEEKTKKLQVMKNSLESDVIDKQKELETVKQQVES
                     ESEKLQLIGQRQVELEKRVEQMQKELDSATDRVPNEPVKIPFLRKEVVVEVQDKMFGKA
                     EITKKQTRNYVLSPEQYQEVTKQVNAAVTIKKDYECLRETDFVKEYESLKMTAEGWMKE
                     NRTLKQEKKQLQKEVGVLNREISSLKAHIKGLQTNIRVLYVQTKKVFKEQFKAFREIVK
                     NELDSKGIDNQFEREHKREISRHRDFDRER"
                     /name="plasmid recombination protein"
     gene            2778..3686
                     /locus_tag="WHT_RS28955"
                     /old_locus_tag="WHT_58450"
     CDS             2778..3686
                     /locus_tag="WHT_RS28955"
                     /old_locus_tag="WHT_58450"
                     /inference="COORDINATES: protein motif:HMM:NF022240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2599 domain-containing protein"
                     /protein_id="WP_050267883.1"
                     /translation="MKSKIMKLIVVGSMTGTIFGVANNVSAQSNNVLDSNQYKNVLNQN
                     MNSEPKYLSFSTAEGETIKIGAMKSSDSKYGNSEIYKFNSSSNEKEIAPKIKKIENGVE
                     QIIEIEPLNANTDYNIPFDFQNGESIKLEKDGSAVIYNKTKDSIAVIMPTEAKTKSGKT
                     LEVKTKLINKNTLSYHVNFKGETEPVTIAAKALSYSFSQYFSSGKWITRDGMLSLSLQP
                     TSLLTKVNSNPNVNGAIRTDSWNKVKNKFSGSGNWKNTGSMENQYYCHVDTAQRFKTPW
                     NLEPHRPNVGYTQTVKKLCNP"
                     /name="DUF2599 domain-containing protein"
     gene            3772..4077
                     /locus_tag="WHT_RS28960"
                     /old_locus_tag="WHT_58460"
     CDS             3772..4077
                     /locus_tag="WHT_RS28960"
                     /old_locus_tag="WHT_58460"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050267881.1"
                     /translation="MENLTFVYFQPILISIVVLLILLSLFLLLKKNIDKSSKISCIIFS
                     VIALYFSGLLIIIEGTIIDELNLKGDQKSFYLSIFGIGISIINLIIAVMKKNGVKH"
                     /name="hypothetical protein"
     gene            4125..4577
                     /locus_tag="WHT_RS28965"
                     /old_locus_tag="WHT_58470"
     CDS             4125..4577
                     /locus_tag="WHT_RS28965"
                     /old_locus_tag="WHT_58470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017561684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="WP_050267879.1"
                     /translation="MKKMLIFSIIVVCTMILFFLFQKEKFTNDKEVVKSQMSENESENY
                     YSKQLTLDQLQTALQNKEEKIVYFYKPNCPYCEEVSPLIVPMAKKENLDLEVLNLDSYK
                     EGWSHYKIEGTPTIVHYKGGKEVNRIVGATDENGYLEWFRSLKKTI"
                     /name="thioredoxin family protein"
     gene            complement(4896..5456)
                     /locus_tag="WHT_RS28970"
                     /old_locus_tag="WHT_58480"
     CDS             complement(4896..5456)
                     /locus_tag="WHT_RS28970"
                     /old_locus_tag="WHT_58480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421195.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="WP_050267878.1"
                     /translation="MELKSEMHTPKIVADLLGITPDLLKVWSNEFNIQTERSQGGHRRY
                     SKENIEELKAIKEKIQAQKWSYDQVRSWRNGELDLFVSKEEKSELEKKLNEVLENQQLQ
                     NQFNQALVQKLQEITNELVTTKEHLANTEKKLTEVEGKNSELEVFIEKKLEKRDQLLLE
                     NIRDMQAQNQKQKRKGFFGLFKK"
                     /name="MerR family transcriptional regulator"
     gene            complement(5912..6220)
                     /locus_tag="WHT_RS28975"
                     /old_locus_tag="WHT_58490"
     CDS             complement(5912..6220)
                     /locus_tag="WHT_RS28975"
                     /old_locus_tag="WHT_58490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002035461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="WP_072770324.1"
                     /translation="MLVLMIMLPISLVPVCYFFGYYGFIALLVLSILLMFASLKVESIK
                     REHNLKTYREIVDFVEGKPVNKVKVDKKNRVLKTSLILLIPLISYIVVYLGMTAFGV"
                     /name="transcriptional regulator"
     gene            6486..7037
                     /locus_tag="WHT_RS28980"
                     /old_locus_tag="WHT_58500"
     CDS             6486..7037
                     /locus_tag="WHT_RS28980"
                     /old_locus_tag="WHT_58500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003466634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_000028030.1"
                     /translation="MSISKHANKKKNILIAASEIVKEEGVVKLTLEAVAQRAGVSKGGL
                     LYHFPSKEALIKGMVEDWTNNYFECINTLVNNDDDNAIGKWNRAYLKSTFSDLENNNLN
                     SALMATMFINPDLLDEFRQRYDILHTKLITDGIDPVKITITRLSIDGLWFSEIFGMAPL
                     NEELKTQVFDELINMIQEDE"
                     /name="TetR/AcrR family transcriptional regulator"
     gene            7037..7414
                     /gene="qacH"
                     /locus_tag="WHT_RS28985"
                     /old_locus_tag="WHT_58510"
     ARG             7038..7411
                     /name="qacH"
                     /gene="qacH"
                     /gene_name="quaternary ammonium compound efflux SMR
                     transporter QacH"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(<7667..7894)
                     /locus_tag="WHT_RS28990"
                     /old_locus_tag="WHT_58520"
                     /pseudo=""
     CDS             complement(<7667..7894)
                     /locus_tag="WHT_RS28990"
                     /old_locus_tag="WHT_58520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020600.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /name="helix-turn-helix transcriptional regulator"
     gene            8244..8450
                     /locus_tag="WHT_RS28995"
                     /old_locus_tag="WHT_58540"
     CDS             8244..8450
                     /locus_tag="WHT_RS28995"
                     /old_locus_tag="WHT_58540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002084334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_057598022.1"
                     /translation="MKSFGMLVCSTIFSVLLVYYNIHSFYNKFTSGNTYYWVNGILAAG
                     FLISLIINIKDIIKKNYTTSETN"
                     /name="hypothetical protein"
     ARG             7038..7414
                     /name="qacG"
                     /gene="qacG"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="60.75"
                     /coverage="116.82"
     Typing          complement(411..1873)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.959"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 acccgatctg taggtttttt ctttttccct atgaaatact catctccacc agttgtttta
       61 cttaataact gcaagagcat tgctttctct tcttctgagt aaccaccggc ttgcaactgg
      121 tcataaatac tatcaatctt actatctctt attttcgcct ttttttcagc ttgtccaaaa
      181 tctacaactt ttgtcataaa ataattctct cccttagtgg aaggaatcaa cttttatatg
      241 ttagaatgta tatagaatat atattggttg atcccaacct tattatgaaa aaccgccttg
      301 ccgtccaaag cttgggcggt ttttctcgtt tgtatgtctt tctctataat aacatgaaac
      361 accttgttaa agctttccgt ctcttgcaaa aatacaacat tcaatactat ctttccctat
      421 caaaatctcg atgtctgctt atttctctct tatgttcacg ttcaaattga ttatctattc
      481 ccttactatc caattcattt ttaacaattt ctctaaacgc cttaaattgc tctttaaaaa
      541 cttttttcgt ttgtacatac aaaactctta tattcgtctg caaacccttt atatgagctt
      601 ttaatgagct aatttctcta tttaatacgc caacttcttt ttgcaactgt tttttttcct
      661 gtttcaatgt tctattttct ttcatccaac cttctgcagt catttttaaa ctctcatact
      721 ctttcacaaa gtctgtttct ctcaaacact cataatcctt tttgatagtg accgctgcat
      781 tcacttgctt tgttacttct tgatattgtt ctggtgataa cacataattt cttgtctgtt
      841 tctttgtaat ttcagctttt ccaaacattt tatcctgtac ttctactact acttctttac
      901 gtaaaaatgg aattttaaca ggttcatttg gcactcgatc cgtagcacta tctaattctt
      961 tttgcatttg ttctactctt ttttctaact ctacctgacg ctggccaatt aactgcaatt
     1021 tttcactttc actctctact tgttgtttca ctgtctctaa ttctttttgt ttatcaatta
     1081 catcactttc caaactattt ttcattactt gtaatttttt tgtcttttct tcaacgtttt
     1141 tcaaatcatg atcaatcgtt tgtaattctg tttctttttc ttgtacttga ttttcaatag
     1201 ttgacttcgc ttctgcatat tccttgtatt gacggacttt catgtactta tgtgaaccta
     1261 cattagcacg ttcaaaattc ggaatgtgtt ctttagccaa ttgctcaata taggccgttt
     1321 ctaattctct ccaatgtgca attaactttc ttcccgttcc ttctacacct tgttgctgca
     1381 aagctctatc cattccgaca cgcctcgtta atcctcgact actttcgaaa tttggtacat
     1441 agttaatatg caaatgcgga ttcgcttcat catcatgtaa aaccatgtta taaaccgcta
     1501 ggtttggatt ccgctcttga aacgcttcag cataccgttt taacgcttct ttcttagcta
     1561 ccctatactt tgggtcatct ttaccttctc caatcgctac gaccaattct cgttgctcat
     1621 gggtcttatc atttttatgt attttgtcat agtaattctc aatcttacgg tcattccgtt
     1681 tttgcttctc gttatacttg tccaccgctt cttgaaagac atccttgtat acacttcgga
     1741 tgtccttttg aacaaagtaa atattctcct ctgacctagc tggatcaatg ccaggattcc
     1801 catgaatatt ttccctatta ttgtggctta atgaactttg gtgagattga ttaaatgaga
     1861 tagaacccaa cataaagtgc caccactttc cctagattaa ttgataccat aagtctagca
     1921 aagcgacgaa atggtgtcaa ttaacgtaac cctatacgta ttttgacgct acgcatcaaa
     1981 atcgataggg gtctgcgacg cttttcggcg ttgccgagcc atgtttagca tggcaaaaac
     2041 aaaccttggg ctttgcccaa acccactggg gaactcttcc ccagacccca tcaatcgaac
     2101 tccccaaccc ctcaatcctt gaggggctcc ctcgccggtt ggcaggaaca aacggccgtt
     2161 tgttcaatac caagagggtt cgggtgtagt ctagttggtc aagtcaaata ctcccttcgc
     2221 ttcgctaggt ccgttttgat tgaccaacac acgcaacgac ctctactgat cactttcact
     2281 ttgctctctt tattacccta aaacgaaaaa aagtcctaaa ttaataggac tctttctcat
     2341 tctatcgaaa gtaaactttt caatattagt ttacattcaa atcctcttaa tcattgactt
     2401 ctcaccaaat tccctaatac cctcacaaaa tgaacatgaa aagtatacat acaaaacgct
     2461 ataaataagg ggattaaaaa ctaaaaatca aattcccctc ctatgtaaaa cccttcaaaa
     2521 ttcaaaacat ataaaagctt taactttaaa cttattaaac aaaaaagaat tatctatatt
     2581 aaaaatttgt gtagtaattg aacagaaata gctaaagatt ccgcaaggaa caggtcatat
     2641 tttctaatat gtaatataaa tcgatatgta tttcccaaaa atatgtcaat ttatattaca
     2701 atgtattcca attattcaaa aaatatggta acctaataat tggcaataaa tcttataaaa
     2761 aatggggagg cattaaattg aaaagtaaaa tcatgaaatt gattgtagta ggatctatga
     2821 caggaactat ttttggtgtt gcaaacaatg tgtcggcgca atcaaataat gtattagata
     2881 gtaatcaata taaaaatgta ttaaatcaaa atatgaattc tgaacctaaa tatttatctt
     2941 tttctactgc tgaaggagaa acaattaaga ttggcgctat gaaaagttct gattctaaat
     3001 atggaaattc agagatttat aaatttaata gctcctcaaa tgaaaaagaa atagcaccaa
     3061 aaatcaagaa aattgagaat ggtgttgagc aaataatcga aattgaaccg ttaaatgcta
     3121 atacagatta taatattcca tttgattttc agaatggaga gtctataaaa ttagaaaaag
     3181 atggctctgc agttatctat aataaaacta aagattctat tgctgttata atgcctacag
     3241 aagcaaaaac aaaatcaggt aaaacactag aagtaaaaac taaacttata aataaaaata
     3301 cattatcata tcatgtgaat ttcaaaggag aaactgagcc cgtaacaatt gcagcaaaag
     3361 ctctatctta ttctttttct caatattttt ctagtggtaa atggattaca cgagacggta
     3421 tgctttcctt aagtttacaa cctacatcac ttttaacaaa agtaaatagt aatccaaatg
     3481 taaatggtgc tattcgaaca gacagttgga ataaagtaaa gaataaattc tcaggaagtg
     3541 gtaattggaa aaatacagga tccatggaaa atcagtatta ttgtcatgtg gatactgctc
     3601 aaagatttaa aacaccttgg aatttagaac cacatcgtcc aaatgtaggt tatacgcaaa
     3661 ctgtaaaaaa actttgtaat ccataaactt taactgctgg aaagtataac tatacatagt
     3721 tatactttct tgttttatat ttgttaataa tatttaattg gaggaattaa tttggaaaat
     3781 ttaacatttg tatattttca acctatatta atctcaattg ttgtcttatt aattttacta
     3841 tcattgttct tattattgaa aaagaatatt gataaaagtt caaagatttc ttgcatcatt
     3901 ttttctgtta tcgctctata tttcagtgga cttttaatta ttattgaggg aacaattata
     3961 gacgaactca atcttaaagg tgaccagaaa tctttctatt tatctatttt cggtattgga
     4021 atttctatta ttaatttaat tatagcagta atgaaaaaaa acggagtaaa acattaatag
     4081 gaattgttac tcttaaatac aatctttttt cttgaggtga aaaaatgaag aaaatgttaa
     4141 tttttagtat tattgttgtt tgtacaatga tacttttttt tctgtttcaa aaagagaaat
     4201 ttacaaatga caaagaagta gtaaaatccc aaatgtcaga gaatgaaagt gaaaattatt
     4261 attcaaaaca attaacatta gatcaattac aaacggcatt acaaaacaag gaagaaaaaa
     4321 ttgtatattt ttacaaacca aattgtcctt attgtgaaga agtttctcct cttatagttc
     4381 ctatggcgaa aaaagaaaat ttagatttag aagtactcaa tcttgattca tataaagagg
     4441 gatggagtca ttacaaaata gaaggaacac caacaatagt ccattataag ggtggaaaag
     4501 aagtgaatag aatagtaggt gctacagatg aaaatggcta tttagaatgg tttcgttcct
     4561 taaaaaaaac aatttaaata taaataataa ctattctagg attctaaaaa aagaactcca
     4621 agtataggaa tcatagcatg aaatattcac tcaactacaa tactatatat taaatatatg
     4681 attgaaaata atatcatatg tttttttaac actagatata ttaatcttaa ttagtatgac
     4741 atatatagca gtaacaacat taaaatatta ttaacatctt tatagcaagg gaatgcttgt
     4801 agactgatct atactgcatt cccttattgt atgtatgcta tatttctatt cttctttttc
     4861 cgacattaga accctacata aaataccagc caaatttact ttttaaacaa accaaaaaaa
     4921 cctttccgtt tttgtttctg attttgtgcc tgcatatctc taatgttttc aagcaataac
     4981 tgatctcgtt tctctagctt cttctctata aaaacttcta attcactgtt tttcccttct
     5041 acttcagtta actttttctc cgtattcgct aaatgttctt ttgtcgtaac caattcattc
     5101 gtaatttctt gtagtttctg aacgagtgct tgattaaatt gattttgtaa ctgttgattt
     5161 tctaatactt cattcagttt cttttctaat tctgattttt cttcttttga aacaaacaaa
     5221 tcaagttctc cgtttcgcca tgaacgaact tgatcgtaag accatttttg ggcctgtatt
     5281 ttttctttta ttgcttttaa ttcttctata ttttccttcg agtatctacg atgtcctccc
     5341 tgacttcgct ccgtttgtat attaaattca tttgaccata cctttaacaa atcgggtgtg
     5401 attcctaaaa gatccgcaac aattttcggt gtgtgcattt ccgattttag ttccaatttt
     5461 tattcctccc caaaaaacgg aatacgtttt ttacttaaaa aacacgatta aaccaacatt
     5521 ccaatcaaaa tcggaatgta cttacaaccg atatgaaaac cgattttaaa aacgatgtaa
     5581 tttccgaaat acattccgat tttaccaccg atatgtatta cttctcgata ataacatcaa
     5641 aatctttttt ctatatacaa gattttatac aaaaaaagaa gagtgcgaat ccctagaatc
     5701 cccgctacat caacgatgta taaaatcctg tataccaaat aaacagacaa aaaaagaaga
     5761 atccccgtaa atacaaggat tctatcaaaa tagttgctac cgataatgag acattatgtt
     5821 aacttaataa gaatatttat tcacaataaa aattaaagag agtaacaaaa tgttactctc
     5881 atcagaaaaa atcaaatcaa tattttctaa attatactcc aaacgcagtc attcctaaat
     5941 aaactacaat ataactgatt aaaggaatta aaagaatcaa acttgttttc aatactctat
     6001 tttttttatc aacctttact ttatttacag gttttccttc tacaaaatca actatttcac
     6061 gataagtttt taaattatgc tctcttttta tactttcaac tttcaatgat gcaaacataa
     6121 gaagaataga aagtaccaaa agggctataa aaccataata accaaaaaag taacatacag
     6181 gtacaagaga aataggaagc ataatcatta atactaacat gatataactc cacttaaggg
     6241 gtagagtcct gaaaatgcgg atttacaacg gtaatgtaat tttcacccat aaataaagcc
     6301 gattttaaga aatcgactgt tgaactaaag ctccctttac tttaagtaca tttcttcaca
     6361 atctttagat taatttattg gattattttt atgtgaaaga gtgatattaa ataagagctt
     6421 taattcgatt gacaaaaaaa ccgtccagac ggtatattat atgtatctaa gttatggagg
     6481 ttattatgtc tatttcaaag catgctaata agaaaaaaaa tatattgatt gctgcatctg
     6541 aaattgtaaa agaagaagga gttgtcaaac tcacattaga ggcagtcgcc caaagagctg
     6601 gcgtgagtaa aggtggatta ttataccatt ttcctagcaa ggaagcatta attaaaggaa
     6661 tggtggaaga ttggacaaat aattatttcg aatgcattaa tacgttagtt aataacgacg
     6721 atgataacgc aataggaaaa tggaataggg cttatcttaa atctacattt tccgatttag
     6781 aaaacaataa tctaaattcc gcactaatgg ctactatgtt tattaatcca gatttattag
     6841 atgaattccg gcagagatac gatattttgc atacaaagtt aataactgat ggaattgatc
     6901 ctgtcaaaat aacaattaca agattatcca tagatggtct ttggttttca gaaatatttg
     6961 gaatggcacc attaaatgaa gaactaaaaa cacaagtttt tgatgaacta ataaacatga
     7021 ttcaggagga cgaataatgt catatctata tttagcactt gcaatagtgg gggagattat
     7081 tggctcttcc ctattaaaag catctgaagg cttttcaaaa ctctatccta caataggggt
     7141 aataatagct ttcgttggtt gttttttctt tttatccttg tcgctaaaaa caatcccctt
     7201 aaacactgca tatgcattat gggcaggttt aggacttgtt ttgactacag taatttcagt
     7261 tttagtttgg aaggaaaaac ttaacatggc tagcattgca ggaataacac tgattcttgt
     7321 aggagttgta atattgaatt tgtttggacc aggacatgga gaatctggtc atgaagcaag
     7381 cgaaactatt acaatgaatg agacaatgga ataacacata aaaaagtcct tcttaataaa
     7441 gagaaggact ttttagctta attaagggta ttttttgaag tccttattca acaaacctgc
     7501 gtacgttagt ttaagtgtat cacttcacaa ttccaataga gtagagttta ttgagacata
     7561 atgaaattag cgttttaaat gacttctgac tgtctcataa gacttgtatc aaatgcttac
     7621 cgttgtaaat ccgcatttcc ctgacgctac cccacttaag aaaaattgat ttttgaagtt
     7681 tttctttcat gatttttaca tctcctttaa ctaaatcatc tagggagaca tcaaagagaa
     7741 tactcatcat taataaatta tgaatatctg gataatttct ttcattttcc cagttagaaa
     7801 tagattgtct ggaaacatga agtttttctg ctagctcttc ttgggacata ttatcccgtt
     7861 tacgaaaata ttgtatctgt ttactaatgt tcatctatac atctccctta agttcaaggt
     7921 taagttaaag gctaattaaa gactaccaaa tttctttata atagcatgat tctacagtgt
     7981 aaaaatgttt tatcattcta ttttaccttt atttttaggt ataagtgctg gatcagctaa
     8041 tacaattgct gacccaaatt taagcaacta tttaaaaaat aaaacttttt tggatgtgaa
     8101 aagtttactt ttacttccta caacggtagt tatgtaaata agctgtccat ctggacagct
     8161 tattgtatgt tttgcttaaa atctctttat ttacatgtat aattataaca aaagttacca
     8221 attttagaat tgaggtgaac agtatgaaaa gttttggtat gttagtatgc tctactatct
     8281 tttcagtgct tctagtatat tataatatcc attcctttta taataaattt acatcaggga
     8341 atacatatta ttgggtaaac ggtattctag ctgccggatt ccttatatcc ttaattataa
     8401 atataaaaga tatcatcaaa aaaaattaca caacttctga aactaattaa gaagcctatt
     8461 atggcttctt tttctattca aaaacatagt ttttaaggtt tgacatgcca aacccttagc
     8521 gtgcctattc cttttttttg gatcttgccc tttggtcttc cattgctttt tggctagagt
     8581 gctggctcgt cgtgtggggg cgaaacccca cgtattacgt atctattttt gaataaagat
     8641 tattttttgg acgaatgatc taagcggttc ggcttgtata cttgctagtc caagtcgaga
     8701 accattactt tgctttttgt ctgtcggctt ggcgagaaaa gcacacgagc cgaaaaaagt
     8761 tctgcaagga cagagaaaaa aagaaaaggc gatagccttt aggtgaggat taacgaaccg
     8821 actttttttc tcctgaaggt ggagattttt gagcgttgtt tgctcaaaaa tactacctgt
     8881 tccttgcgga acttttagcc ctatatataa gagtgagata gagcaaataa aaaaagccag
     8941 taataccaag ggtttatagc atatgaggtg ttactttttc gtaacacctg tgttactttt
     9001 tcgtaacacc taataatagg aaaaacataa taaaaaaaag atgtcaatga gaatttgaca
     9061 tctaaaataa atagattggt aatttatatt ttttcttaga ctcatcaatt ttaaagttcc
     9121 ttaatgaatt tctaaaaata tgttgagtcg ctttatctat tccatcttta ggactacaac
     9181 acataatatt aggatttaca aaccatgtac gacttgtaca tgcacgacca tcttctgttg
     9241 ttattccagc tgcggcaact cctaaaaccc cttttttaaa taaaccattc attgttaaag
     9301 aaacattttt tcttgccatt ttaaaacgtt cggctaaata tgtaggtgat gctggagtag
     9361 ttgtatctac attgtcctta tcaatttttt gaataataac atttgtttta aattcaacat
     9421 atggagcaag tttaaataaa aatgcttctt ccttgctact caaatactca atttcagtaa
     9481 gataattaac gttatccata attagctgca caaacctc
//
