LOCUS       NZ_AP024409             4897 bp    DNA     circular CON 23-MAR-2024
DEFINITION  Aeromonas caviae strain KAM376 plasmid pKAM376_7, complete sequence.
ACCESSION   NZ_AP024409
VERSION     NZ_AP024409.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00270046
            Assembly: GCF_016861425.1
KEYWORDS    RefSeq.
SOURCE      Aeromonas caviae
  ORGANISM  Aeromonas caviae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Aeromonadales;
            Aeromonadaceae; Aeromonas.
REFERENCE   1
  AUTHORS   Suzuki,M., Maehana,S. and Kitasato,H.
  TITLE     Whole genome sequencing of carbapenem-resistant Aeromonas spp.
            isolated in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4897)
  AUTHORS   Suzuki,M., Maehana,S. and Kitasato,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2021) Contact:Masato Suzuki National Institute of
            Infectious Diseases; 4-2-1 Aobacho, Higashimurayama, Tokyo 189-0002,
            Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Unicycler v. 0.4.8.0; Canu v. 1.8
            Genome Coverage                   :: 132x
            Sequencing Technology             :: Illumina MiniSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_016861425.1-RS_2024_03_23
            Annotation Date                   :: 03/23/2024 02:24:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,704
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,441
            CDSs (with protein)               :: 4,441
            Genes (RNA)                       :: 160
            rRNAs                             :: 11, 10, 10 (5S, 16S, 23S)
            complete rRNAs                    :: 11, 10, 10 (5S, 16S, 23S)
            tRNAs                             :: 120
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 39 of 103
            Pseudo Genes (incomplete)         :: 64 of 103
            Pseudo Genes (internal stop)      :: 15 of 103
            Pseudo Genes (multiple problems)  :: 13 of 103
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP024409.1.
            Annotated at DFAST https://dfast.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4897
                     /organism="Aeromonas caviae"
                     /mol_type="genomic DNA"
                     /strain="KAM376"
                     /db_xref="taxon:648"
                     /plasmid="pKAM376_7"
                     /geo_loc_name="Japan:Kanagawa"
                     /collection_date="2019"
     gene            complement(102..335)
                     /locus_tag="JSX26_RS23725"
     CDS             complement(102..335)
                     /locus_tag="JSX26_RS23725"
                     /inference="COORDINATES: protein motif:HMM:NF016769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD/MobD family mobilization/exclusion protein"
                     /protein_id="WP_153913308.1"
                     /translation="MTELERHLLNALETLQSEQDRQLSGLQTACESLLRQFETTKKENA
                     SLLGEVKVLRQQCEHLNEQVSSLATQVASLKA"
                     /name="MbeD/MobD family mobilization/exclusion protein"
     gene            complement(339..773)
                     /locus_tag="JSX26_RS23730"
                     /old_locus_tag="KAM376_45750"
     CDS             complement(339..773)
                     /locus_tag="JSX26_RS23730"
                     /old_locus_tag="KAM376_45750"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_153913306.1"
                     /translation="MSKILDMAKTFEQTSKQQADDIESSLKPEFERHERAIKEALDSSR
                     QSINDAIQDQQKLIGWLLLKSWGVMLISGLLLLAATSGTLWYQGNLIAERQATLEALGK
                     KGGKLIFNKCEDENGKIRLCIRMNMDEGKFGDDYMIPKGY"
                     /name="MbeB family mobilization protein"
     gene            complement(<808..1347)
                     /locus_tag="JSX26_RS23735"
                     /pseudo=""
     CDS             complement(<808..1347)
                     /locus_tag="JSX26_RS23735"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1442..1780)
                     /locus_tag="JSX26_RS23440"
                     /old_locus_tag="KAM376_45760"
     CDS             complement(1442..1780)
                     /locus_tag="JSX26_RS23440"
                     /old_locus_tag="KAM376_45760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019820367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_094965905.1"
                     /translation="MPTEQPKRLKTIKVRVTETEWASLRAACPKAQLAEWMREYCLGVV
                     PQQRRTPPPTIDPALLRQVAGIGNNLNQIARRINASPLTTLDKVEYLAALAAVARELEG
                     LRRDRQNP"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1979..2269
                     /locus_tag="JSX26_RS23445"
                     /old_locus_tag="KAM376_45770"
     CDS             1979..2269
                     /locus_tag="JSX26_RS23445"
                     /old_locus_tag="KAM376_45770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005321770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_094965911.1"
                     /translation="MKDNADKGTGDMFGIKRGRGRPKTGSAKTGAERQAAYRTKQQENN
                     VTVTINRGLLEGLDAQMQAIRDGGSAVVLTSEQAGEILRALRTAEKKQLRG"
                     /name="hypothetical protein"
     gene            complement(2422..3408)
                     /locus_tag="JSX26_RS23450"
                     /old_locus_tag="KAM376_45780"
     CDS             complement(2422..3408)
                     /locus_tag="JSX26_RS23450"
                     /old_locus_tag="KAM376_45780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005321767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_094965910.1"
                     /translation="MTEKAFLRNLPHRPYCTDDKSSGLLIRPQATALGYRHIQYNPPPH
                     VASLVFDLDKPDSYHAWQDAGLPAPHWISVNRTNGHAHIGYMLATPVARTSAARQKPLR
                     YLAAIEHVLAKRLGADMGYAGLITKNPTHSDWLTIWHQIEPYSLDYLAEFCPDADLAAY
                     SRRSRKEATGLGRNVTVFDNVREWAYTAIRAHWRPNGYDAWLCAVQAACECTNVFGLEQ
                     GGPLPHSEIKATAKSIARWTWRNLTPSTFADYVDRTHTPEIQAVRGAKGGRIGGKVSKG
                     GGRPSKAASLLPVVLQLKAQGYSNRDIAEDLQISAGSVSNYLRRDRE"
                     /name="replication initiation protein"
     gene            complement(3570..4001)
                     /locus_tag="JSX26_RS23455"
                     /old_locus_tag="KAM376_45790"
     CDS             complement(3570..4001)
                     /locus_tag="JSX26_RS23455"
                     /old_locus_tag="KAM376_45790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019535530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="WP_094965909.1"
                     /translation="MNIPTHLKHKPVLEVINYDQIDGPYANDSDAMGLSIGIAQWNSPG
                     WTDLSAKVWRHTGEKWSRQSEELPLHRVIDLTTLICIAMEYCRNGRLSSSDDFPVSRST
                     DNADLGHHVELMKKELKSGSKYLDESLKRLSLELKRLGY"
                     /name="DUF6530 family protein"
     gene            complement(4116..4424)
                     /locus_tag="JSX26_RS23460"
                     /old_locus_tag="KAM376_45800"
     CDS             complement(4116..4424)
                     /locus_tag="JSX26_RS23460"
                     /old_locus_tag="KAM376_45800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="WP_094965908.1"
                     /translation="MQFTIYRNKGNARAYPYLLDVQSDIIGELATRIVIPLFPLAAFTG
                     RPAQRLNPVIIVEGDKYLVMTQDMAAVRQTQLGEEVTGVQESRQLIKDAIDFLMDGV"
                     /name="CcdB family protein"
     gene            complement(4424..4663)
                     /locus_tag="JSX26_RS23465"
                     /old_locus_tag="KAM376_45810"
     CDS             complement(4424..4663)
                     /locus_tag="JSX26_RS23465"
                     /old_locus_tag="KAM376_45810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="WP_094965907.1"
                     /translation="MVAAAMRSKRTVSVTLEPSLLERARAAGINLSATLAAALQHEISG
                     SEAERWKRENSAAIQELNRITEEHGLLSDDYRAF"
                     /name="type II toxin-antitoxin system CcdA family
                     antitoxin"
     Typing          complement(2..921)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.584"
                     /coverage="100.0"
                     /evalue="3.95e-161"
ORIGIN
        1 tttctgctcg tgcttccgat tgaaatagag atcccgttct gcgtgggcct gctggtattc
       61 gtccaataac ccctcctgcg tctgcgccgt ctgctgcgcg atcatgcctt caagctggcg
      121 acctgcgttg ccaagctgct gacctgctcg ttcaagtgct cgcactgctg gcgcaagact
      181 ttcacttctc ccagcaagct cgcgttttct tttttcgtcg tctcgaactg ccgtagcaag
      241 ctctcgcacg ctgtttgcaa gccgctcaat tgccgatctt gctcgctctg caaggtctct
      301 agcgcgttca ataagtgccg ttctagctcg gtcatcgttt agtagccctt cggtatcatg
      361 taatcgtccc cgaacttccc ttcgtccatg ttcatcctga tgcaaagccg gatcttcccg
      421 ttctcgtcct cgcacttgtt gaagatcaat ttcccgccct ttttgcccaa cgcctccaag
      481 gtggcctgcc gctcggcgat caggttcccc tgataccaca gcgtcccgct cgtcgcggcc
      541 agcaacagca agcccgagat caacatcact ccccagctct tgagcaggag ccagccgatc
      601 agcttctgct ggtcttggat ggcgtcgttg atgctctgcc tgcttgagtc caacgcttct
      661 ttgatagctc gctcgtgcct ctcgaactct ggcttcagac tgctctctat atcgtcggct
      721 tgctgcttcg atgtctgctc gaaggtcttt gccatgtcta aaatcttgct catagatcgc
      781 cccctttagg cggatattcc ggcccccttc agggtcggca atgctgatac tgttcttggt
      841 tgtcctggta acgtcgaacc ccgccccggt cagggtcttg agcacgtcct cccggttttt
      901 aaccagcccc gccgcagcca tgcccagcaa gccgcctgta atggcttgtt cggcttccag
      961 cttgttccgg ggtaggtctt tggccgtagt cacggcctgc cgattgattg ggtcgtctgg
     1021 gtcgtgcaat cccaagtcgc cattgatggc cgttcgccat gcgttgatcc ggtggccgtc
     1081 cgccccgtgg aaatacggct gaaggcgttt gccggacaac agttcgacgt tcggcaccac
     1141 gaaattcagc tctaggcggt ctttgtctcg gtgctcgacc cacagcaccg catactgatc
     1201 ggcgtccagt ccgggcaaca gggcttgctc gaactcgtcc atcaaccgat ccttggtctc
     1261 cctcggcaag tccggttcat ggaacgacag cacgccgctg gtgtaccgct gggcgtaacg
     1321 gctgccgtct atcaactccc tgaccatctc ggggtcgccg cgatccagcg tggccccgct
     1381 ccggttcccg tctttgccaa gcaggtattc caccgggcca gatccccgac cgactccccg
     1441 gctatggatt ttgacgatca cggcgtagcc cctccagttc tcgggccacg gcggctaacg
     1501 ccgcaaggta ttccaccttg tccaacgtgg tcagcgggct ggcattgatg cgccgggcga
     1561 tctggttgag gttgttcccg atcccggcca cctgccggag caaggccgga tcgatggttg
     1621 gcggtggggt gcgccgctgc tggggaacaa cccccaagca gtactcgcgc atccactccg
     1681 ccaactgggc tttggggcag gccgcccgca agctggccca ctcggtttca gtgactctga
     1741 ccttgatcgt tttgagccgt tttggctgct ctgtcggcat cgatggcctc cgtttttttc
     1801 gggggtttcg gggggcttgc cccctgaaca aggccacagg ggatagaccg cgaagcgggc
     1861 cccctgtgtg tccttgctta gtactttaac atgggttgga ttttgagggt gcggattaat
     1921 ggtttttgtg tcacagtgac agataatatt tatggtcact gtgacgggaa ttaggacgat
     1981 gaaagacaac gctgataagg gaaccggaga catgtttggc atcaagcgcg gacggggtag
     2041 acccaaaacc ggatcagcca aaacaggggc agaacgacaa gcggcctacc ggacaaaaca
     2101 gcaggagaat aacgttactg tgacgataaa tagagggcta ttggaaggac tggatgccca
     2161 gatgcaggcg atcagagacg ggggttctgc ggtggtgctg acctcggaac aggccgggga
     2221 gatcttgcgg gcgctacgaa cagcagagaa aaaacagctc cggggataac agctactgcc
     2281 cacaggccga acacatggcc tgatggacag ccctacggga tcgccgttac cccccctcgc
     2341 caagatcctc tctcttgaat gcttttaggg ctcaaggaac gccctacggg cgctgttatc
     2401 tgatataggc tttgtttttg atcactcacg atcacggcgg aggtagttgg acacactgcc
     2461 agccgaaatt tgcagatcct ccgcaatatc gcgattgctg taaccctgcg ccttgagttg
     2521 tagcaccaca ggtaggagag aagcagcttt gctcggcctg cctccgccct tggatacctt
     2581 gccaccgata cgcccgccct tagcccctct gacggcctga atctctggtg tatgcgtccg
     2641 atcaacataa tccgcgaacg tggacggggt tagattccgc cacgtccaac gggcgatgct
     2701 cttggccgtg gccttgatct cgctatgggg caacgggcca ccctgctcca agccaaacac
     2761 gttggtgcac tcacaggcag cttgcacggc acagagccat gcatcgtacc cattaggccg
     2821 ccaatgcgct ctaatcgccg tgtaggccca ttcccgcacg ttgtcgaaca cagtgacgtt
     2881 acggcctagc ccagtcgcct ctttgcgact gcggcggctg taggccgcca aatcggcatc
     2941 cgggcagaac tctgcgagat agtcgaggct gtagggctcg atttggtgcc agatggtgag
     3001 ccaatcgcta tgggtggggt ttttggtgat cagccccgca taccccatgt cggcccccag
     3061 ccgcttcgct agaacgtgtt ctatggcggc taggtatcgc agcggctttt gccgggcggc
     3121 gctggtacgg gctactgggg tcgccagcat gtacccaata tgggcgtggc cgttcgtgcg
     3181 gttaacgctg atccagtgcg gggctggcaa gcctgcatcc tgccaagcgt ggtaactgtc
     3241 cggtttatcc aagtcgaaca ccaaagacgc cacatgcggc ggtgggttgt actggatatg
     3301 gcgatagccc agtgcggtag cctgcgggcg aatgagcagc cctgacgatt tatcgtcagt
     3361 gcaatagggc cgatggggga gattcctgag aaaggctttt tctgtcatcg atcaccgcgc
     3421 ttattggcgg tgcagggttg tttggtgtga tccatgtggt agacttctct tgtgattcgt
     3481 cacagccccc gtttttcgca gatccgccaa gattccagcg ataacggggg tttgtctttt
     3541 tatgagcaat aagttaccgc atagccccgt tagtaaccca gcctttttag ctctaaagac
     3601 agtcgtttta gactttcatc gagatattta ctcccgctct tgagctcttt tttcattagc
     3661 tcaacatgat gccccaaatc agcgttatca gtagacctgc taaccgggaa atcgtcgcta
     3721 gatgacaagc gaccattgcg acagtattcc atcgcgatac atatcaatgt cgttaagtcg
     3781 ataacacgat gcagtggtag ttcttctgac tgtcttgacc atttctcgcc tgtatgacgc
     3841 catactttgg ctgagaggtc agtccatcca ggtgagttcc attgtgcgat gccaattgac
     3901 agtcccattg catcagagtc attagcatat ggaccatcaa tctggtcata gttaataact
     3961 tccagtaccg gcttgtgctt caaatgagtt ggaatgttca tagcgagccc cagattatca
     4021 agtttactaa tttcacaaac attactgagt ttagttggta atgtctatgg ggttgtacgc
     4081 cggaaccgcc gaaatatctg cctcccttta aagcctcaaa caccatccat caggaaatct
     4141 attgcgtcct tgatgagctg acgagactcc tgcactcctg tgacttcttc cccaagctgc
     4201 gtctgcctca ccgctgccat gtcctgcgtc atcaccagat acttatcccc ctcaacgata
     4261 attaccggat tcagccgttg cgctggccgc ccggtgaagg ccgccagcgg gaacagtggg
     4321 atcactattc tggtggccag ctcgccgatg atgtcgcttt gcacatccag cagatagggg
     4381 taagcgcggg cattgccctt gttgcggtag atcgtgaact gcatcagaac gccctgtaat
     4441 cgtcggaaag caaaccgtgc tcctcggtga tgcggttaag ctcctgtatg gctgcactat
     4501 tctcacgctt ccagcgttct gcttctgatc cgctgatttc atgctgtagt gcggctgcaa
     4561 gggtggcgga caggttgatc cccgccgctc tggctcgttc cagcaagctg ggctccagcg
     4621 tgacgctgac cgttctcttg ctgcgcattg ctgctgcgac catgatgacc tccaataaat
     4681 taacacgtgc gatagcacgt gtatgataat atcacgccct tgcgacgggt gcgactagcg
     4741 aagtctgtcg cgctcggggc aggggccgcc aagcgcagcg cggcagctac atctcaaagc
     4801 cacgcccccg ttcgcggacc tgctgccgct ctatccgccc ctccagtcct tcctgtaggt
     4861 actctctggc cttgtctatc ggcaagccgg agatctc
//
