LOCUS       NZ_AP024407             8541 bp    DNA     circular CON 23-MAR-2024
DEFINITION  Aeromonas caviae strain KAM376 plasmid pKAM376_5, complete sequence.
ACCESSION   NZ_AP024407
VERSION     NZ_AP024407.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00270046
            Assembly: GCF_016861425.1
KEYWORDS    RefSeq.
SOURCE      Aeromonas caviae
  ORGANISM  Aeromonas caviae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Aeromonadales;
            Aeromonadaceae; Aeromonas.
REFERENCE   1
  AUTHORS   Suzuki,M., Maehana,S. and Kitasato,H.
  TITLE     Whole genome sequencing of carbapenem-resistant Aeromonas spp.
            isolated in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8541)
  AUTHORS   Suzuki,M., Maehana,S. and Kitasato,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2021) Contact:Masato Suzuki National Institute of
            Infectious Diseases; 4-2-1 Aobacho, Higashimurayama, Tokyo 189-0002,
            Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Unicycler v. 0.4.8.0; Canu v. 1.8
            Genome Coverage                   :: 132x
            Sequencing Technology             :: Illumina MiniSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_016861425.1-RS_2024_03_23
            Annotation Date                   :: 03/23/2024 02:24:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,704
            CDSs (total)                      :: 4,544
            Genes (coding)                    :: 4,441
            CDSs (with protein)               :: 4,441
            Genes (RNA)                       :: 160
            rRNAs                             :: 11, 10, 10 (5S, 16S, 23S)
            complete rRNAs                    :: 11, 10, 10 (5S, 16S, 23S)
            tRNAs                             :: 120
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 39 of 103
            Pseudo Genes (incomplete)         :: 64 of 103
            Pseudo Genes (internal stop)      :: 15 of 103
            Pseudo Genes (multiple problems)  :: 13 of 103
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP024407.1.
            Annotated at DFAST https://dfast.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8541
                     /organism="Aeromonas caviae"
                     /mol_type="genomic DNA"
                     /strain="KAM376"
                     /db_xref="taxon:648"
                     /plasmid="pKAM376_5"
                     /geo_loc_name="Japan:Kanagawa"
                     /collection_date="2019"
     gene            415..1116
                     /locus_tag="JSX26_RS23330"
                     /old_locus_tag="KAM376_45520"
     CDS             415..1116
                     /locus_tag="JSX26_RS23330"
                     /old_locus_tag="KAM376_45520"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_202979151.1"
                     /translation="MEKGGELKTRLSERLSQRQAEELAEVATLTEANLTQLSERLTKHV
                     SAELSTTRDAIRQQSEQITTLLGSQQSALEQQQATVDALKRLSLKDLEQIQKGLNDAIT
                     LAQGKAIEQAIDPLRTALEQLKMDGEALSRVTFRGWLKPLLISMAVLLGAGGSTWGVAA
                     YLAKEITGQLTELQNLNQQIGTAQRTLDSLPQGVTFVRDRDGRYIVARQIDTPFKMDEG
                     TYKGLMAVGVK"
                     /name="hypothetical protein"
     gene            1117..1458
                     /locus_tag="JSX26_RS23335"
                     /old_locus_tag="KAM376_45530"
     CDS             1117..1458
                     /locus_tag="JSX26_RS23335"
                     /old_locus_tag="KAM376_45530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011116720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WP_202979152.1"
                     /translation="MTELERLLTAQFERMEQRHQDETKALRAQLDAQQQSLEGLQTQLQ
                     STLGEYETHCASLHTAFVALEKSTRRELASMGRSLDQTNRATGAALGSLNSSVSIVKSS
                     LESLNKAQS"
                     /name="mobilization protein"
     gene            1558..1845
                     /locus_tag="JSX26_RS23340"
                     /old_locus_tag="KAM376_45540"
     CDS             1558..1845
                     /locus_tag="JSX26_RS23340"
                     /old_locus_tag="KAM376_45540"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_128274296.1"
                     /translation="MKSGRLGTPDTVAHIRQFPPVEYELASYQGSPWLWADSEPLIDLL
                     DATIDWMNDDDGSRWIVIDTEYLRQHVAHYALLSADTWRQRLLDAGVEVD"
                     /name="hypothetical protein"
     gene            1853..2053
                     /locus_tag="JSX26_RS23345"
                     /old_locus_tag="KAM376_45550"
     CDS             1853..2053
                     /locus_tag="JSX26_RS23345"
                     /old_locus_tag="KAM376_45550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005321951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_128274297.1"
                     /translation="MSDRFPGLMLWHTVAVMAAVFAAKALLYWAADWLGLGPLYRALAG
                     PSWDVAAWIAALALLLRWMRV"
                     /name="hypothetical protein"
     gene            complement(2348..3925)
                     /locus_tag="JSX26_RS23350"
     CDS             complement(2348..3925)
                     /locus_tag="JSX26_RS23350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting chemotaxis
                     protein"
                     /protein_id="WP_128274299.1"
                     /translation="MRQNLPVTGRNLELPKDANILSTTSPQSHITYVNPDFIKISGFTE
                     EELLGQPHNIVRHPDMPPAAFEHMWSTLKSGRSWMGLVKNRCKNGDHYWVSAYVTPIAK
                     NGSIVEYQSVRTKPEPEQVLAAEKLYAQLRSGKAARPKLAASFSVKILLLIWGSIISSA
                     MAAGMLTDTSISSLLLATLMSGSLSSVSVLAILSPLGRLVERARNISNNPLSQSLYTGR
                     TDEFGQIEFALRMMQAETGAIVGRIGDASNRLSEHTRGLLKDIESSNVLTVEQQAETDQ
                     IATAVNQMVASIQEVASNAQHAADAAGRADTETASGQRLVAHTSQSITALEGEIRQAAQ
                     VIHELEGQSNEISKVLDVIRGIAEQTNLLALNAAIEAARAGEQGRGFAVVADEVRSLAA
                     RTQQSTTDIQSMISALQERAQSAVTVMEQSSRQADTSVAHAEEAATALDGIGQLVNEIT
                     DMNAQIATAVEQQGAVSEDINRSIINIRDAADTNVQTGQNNLQSAKSVAQLTSALSELA
                     KQFWEKRG"
                     /name="PAS domain-containing methyl-accepting chemotaxis
                     protein"
     gene            4309..5289
                     /locus_tag="JSX26_RS23355"
                     /old_locus_tag="KAM376_45580"
     CDS             4309..5289
                     /locus_tag="JSX26_RS23355"
                     /old_locus_tag="KAM376_45580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416498.2"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="WP_223930743.1"
                     /translation="MSHQLTFADSEFSSKRRQTRKEIFLSRMEQILPWQNMVEVIEPFY
                     PKAGSGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLFARLSLDSALPDRTT
                     IMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQRDPE
                     MHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVSADAG
                     YQGAPQREELAEVDVDWLIAERPGKVKTLKQHPRKNKTAITIEYMKARIRAKVEHPFRI
                     IKRQFGFVKARYKGLQKNDNQLAMLFTLANLFRVDQMIRQWERSH"
                     /name="IS5 family transposase"
     gene            5530..5769
                     /locus_tag="JSX26_RS23360"
                     /old_locus_tag="KAM376_45590"
     CDS             5530..5769
                     /locus_tag="JSX26_RS23360"
                     /old_locus_tag="KAM376_45590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005740683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_016946643.1"
                     /translation="MSVMSVRLPDEVEQQLGQLAQSTGRTKSWLANQAIQDYLAREAWQ
                     ITEVEAALREADAGDFVPEKEMMAKFNRWGINAG"
                     /name="CopG family ribbon-helix-helix protein"
     gene            5759..6043
                     /locus_tag="JSX26_RS23365"
                     /old_locus_tag="KAM376_45600"
     CDS             5759..6043
                     /locus_tag="JSX26_RS23365"
                     /old_locus_tag="KAM376_45600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017411299.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_042650712.1"
                     /translation="MQVKWLRKAALNLEDAHDHLAKENSRVAQEFVLEVYRLVHLLVDQ
                     PAMGRPGRVPGTRELVLQPYPFLIPYRVKGEEIQILRVFHTRLRLPSHW"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            6253..7197
                     /locus_tag="JSX26_RS23370"
                     /old_locus_tag="KAM376_45620"
     CDS             6253..7197
                     /locus_tag="JSX26_RS23370"
                     /old_locus_tag="KAM376_45620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_042650711.1"
                     /translation="MNRAQLDLFRERLPRKPYHTDEFTTGLSIADVSRALGARYIQPNG
                     PTHRHWLVFDVDHAAATLSWDDVGAPAPNITVTNRANGHAHLIYGLETPIRTAPDGKVA
                     PLRYAAAIEAALREKLGADLGYSGLICKNPLHTHWLVQVWEPASYDLSWLADYLDLSPY
                     NGRKQLPAYGLGRNCTLFEKTRQWAYKAIRQGWPGYEAWLSAVIDRAIGYNVQFEPPLP
                     ANEVRHIAKSIAKWTHRNLTPAGFSAVQAARGAKGGKVSKGGGRPSNSGKARADLLPEV
                     LRLKGMGYSNRDIGEDLGISSSTVSLYLKRESE"
                     /name="replication initiation protein"
     gene            complement(7347..7637)
                     /locus_tag="JSX26_RS23375"
                     /old_locus_tag="KAM376_45630"
     CDS             complement(7347..7637)
                     /locus_tag="JSX26_RS23375"
                     /old_locus_tag="KAM376_45630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005321770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_128274289.1"
                     /translation="MKDTKDQGTGDMFGIKRGRGRPKTGSAKTGAERQAAYRAKQQDNN
                     VTVTINRALVEGLDAQMQVIRDGGSAVVLTPEQAGEILRALRTAENKQLRG"
                     /name="hypothetical protein"
     gene            7850..8251
                     /gene="mobC"
                     /locus_tag="JSX26_RS23380"
                     /old_locus_tag="KAM376_45640"
     CDS             7850..8251
                     /gene="mobC"
                     /locus_tag="JSX26_RS23380"
                     /old_locus_tag="KAM376_45640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005321773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_128274291.1"
                     /translation="MTKPELKTTTASLRLDPATLAKWRKGACDEGLTLSDWLRAKVDAD
                     QQTHIAPARSRRERDPRHLADPALMRQLAALGSNLNQIARAVNECRLVGSPVYLVELLA
                     LLRSIEEAAGTLLPQLPPPPNREGDGHDD"
                     /name="mobC"
     Typing          6261..7197
                     /name="rep_cluster_1195"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.659"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 agcaccggga agcgggaggc ggtgtccacg tccacatctt ggtaccccgt gttcacctgg
       61 gcacgggcaa atctctgaac attgccccgc ccggacatcg aaaggacttt gatgccctgc
      121 gagataaatg gaactacggc aagggttggg ccagaccaga cgacccgctc cgggcacggc
      181 taatacagcc ctcctttgac gcctacaagg gccagaagga caggactgcc atcaagcggc
      241 agataggggg tcacctgctt ggggcggtgg ctcaggggct gatccgcgac cggggcgaat
      301 tactcgagta cctgggcgag gtgctcgaat tcgagatcac ccgccagggc aaggactaca
      361 tcacgatcag gccggaagga tacccgcagg gcatccggct caaaggagag ctgtatggag
      421 aagggtggcg agctgaaaac acgcttgagc gagagactat cgcagcggca agccgaggag
      481 ctggccgagg tggccacgtt gaccgaggcc aatctgacac agctcagcga gcgtttgacg
      541 aagcatgtga gcgccgagct gagtacaacc agggacgcta ttcggcagca gagcgagcag
      601 atcaccacgt tgttaggttc acagcagagc gccttggaac aacaacaggc gaccgtggac
      661 gcgctcaaac gcttgagctt gaaggacttg gagcagatcc agaaggggtt aaacgacgcg
      721 atcaccctcg cccaggggaa ggcaatcgag caggcgatcg atccgctcag gacggcccta
      781 gagcaactca agatggacgg ggaggccctg agtcgcgtga cgttccgggg atggctcaaa
      841 ccgctgctta tatcgatggc ggtgctcctg ggagccgggg gcagtacctg gggggtggca
      901 gcgtacctgg cgaaggagat caccgggcaa ctgaccgagc ttcagaacct aaaccagcag
      961 atcggcactg cccaacggac cctggacagc ttgccccaag gggtgacgtt cgtgcgcgac
     1021 cgagatgggc ggtacatcgt ggcgaggcag atcgatacac cgttcaagat ggacgaaggg
     1081 acgtacaagg gcctgatggc cgtgggggtg aagtagatga ccgagttaga gcggctgctg
     1141 acagctcaat tcgagcggat ggaacaacgg caccaggacg agacgaaagc cttgcgggcg
     1201 cagctcgacg cgcaacagca atctttggag ggcttacaga cgcagttgca gagcacattg
     1261 ggcgagtacg agacgcactg cgccagctta cacaccgcct tcgtggcctt ggagaagagc
     1321 accaggcgcg agctagccag catgggccgg agtttggacc agaccaaccg ggctaccgga
     1381 gcagcacttg ggtcattgaa cagcagtgtg agcatagtca aaagctcgct ggagagcttg
     1441 aacaaggcgc agagttgatc gtcgataggg ccgcggtgct gcgaaaagag cagagccaga
     1501 gtagcgggtt gagcatgtaa tataatgtca cagtgacaat aattaaggag atcgggtatg
     1561 aaatcaggtc ggttgggtac tcctgacacg gtggcgcata ttcgccagtt ccccccggtt
     1621 gagtatgaac tggcgagcta tcaaggctcc ccatggctat gggcggatag tgaaccgctg
     1681 atcgacctcc tcgatgcgac catcgactgg atgaacgatg acgacggcag ccgctggatc
     1741 gtcattgata ctgaatacct gcgccagcat gtggcccact atgccctgtt gtccgccgat
     1801 acatggcggc aacggttgct cgatgcaggc gtggaggtgg attgaggtgc gattgagtga
     1861 tcggtttccg gggctgatgt tgtggcatac ggtggcggta atggccgccg tgtttgcggc
     1921 gaaagccctg ctgtactggg ctgcggactg gttgggcctt ggtccgctct accgcgcctt
     1981 ggcagggcct tcctgggacg tggcggcatg gattgctgcg ctggccctgc tgctgcgctg
     2041 gatgcgggtc taactgcctg ccgcgctgcg cttggcgact cccgaagggg gcacgacaga
     2101 ctgcgctagt cgtccccgtt cgggagtgtg gcctgatcca gaaacaccct gcaggatccg
     2161 ggggaaccgc agaattcgga aaaaatcgta cgctaagcta acggtgttct cgtgacagct
     2221 ctttgactag gctttctaag gccattctga tagccctgac ttcctgaaaa gccatggcta
     2281 aaatttgtgc ggctaaaagg gataccgatg gtaaagtaag ttatccctgt cgagatactg
     2341 aaaagcgtta tcctcgtttt tcccaaaact gttttgccag ttcgctcaga gcgctagtta
     2401 actgagcgac agatttcgca ctttgcaaat tattctgccc ggtctgtaca ttggtatcag
     2461 cagcatcgcg tatattgata atactgcggt ttatgtcttc acttactgct ccctgctgct
     2521 cgaccgcagt cgctatttgc gcgttcatgt cggtaatttc gttaacgagt tggccaattc
     2581 catcaagagc tgtagctgct tcctctgcgt gagctacact cgtgtccgct tgccgactac
     2641 tttgctccat gactgtaaca gcggattgcg ctcgctcttg tagagcgctg atcatgcttt
     2701 gaatatccgt tgtcgattgc tgtgtgcgag cagcaagact gcgaacctca tcggcgacaa
     2761 cagcaaaacc acgcccctgc tcaccagcac gcgcggcctc aattgctgcg ttgagtgcca
     2821 acaaattcgt ttgctcggcg atccctcgta taacgtcaag aacttttgat atctcgttac
     2881 tttgaccttc aagctcatga ataacctgag cggcttgcct aatttcacct tcaagggcag
     2941 tgattgactg gcttgtgtgg gctaccagac gctggccaga tgccgtctca gtgtctgctc
     3001 ttccggccgc atctgcagca tgctgtgcat tgctcgcaac ctcttgaatg cttgccacca
     3061 tttggtttac tgccgttgct atttgatctg tctctgcctg ctgctcaact gtaagtacat
     3121 tgcttgactc aatatccttt agtaggcctc gggtgtgttc gctaagccga tttgatgcat
     3181 cacctatgcg acctactatg gcgcctgttt cagcttgcat cattcgtaaa gcaaactcta
     3241 tttggccaaa ctcatcggtg cgcccagtgt agagggattg acttaatggg ttattggaaa
     3301 tattcctggc tctttcaacc agtcttccaa gaggagagag aatagccaaa acactaacag
     3361 agcttaagct tcctgacatt aaagtggcta acaataagct gcttattgat gtatcagtaa
     3421 gcatgccggc agccattgcg cttgatataa tactacccca tatgagcaag agtattttca
     3481 cggaaaagct agcagccaat ttcggcctcg cggccttccc gcttctcaat tgagcatata
     3541 atttttccgc agccaaaacc tgctcaggtt caggcttggt ccttacagac tgatattcaa
     3601 caatcgaacc attcttagct attggcgtta cataagcact tacccaatag tggtcgccat
     3661 ttttacagcg attttttact agccccatcc atgagcggcc agattttaat gtactccaca
     3721 tatgctcaaa tgcagcaggc ggcatatctg ggtgtcttac gatgttgtga ggctggccta
     3781 atagttcttc ctcagtgaaa ccactgattt taatgaagtc aggattaacg tacgtgatat
     3841 ggctttgagg ggaggtagtc gaaagaatat tggcatcttt tgggagttct aagtttcgac
     3901 ccgtcactgg taaattctgg cgcataagaa cctcaagggt tggctgtttt attttattgt
     3961 tttcggcatt aagcccaatt tcttgagcgt tacgataaag ctaaataact gatcctaata
     4021 gtgctacatc ttgccggaag ccttgtggcg taaggaattt gtgcaatctg acggcaacgc
     4081 ctgaagtgac aaggctcagc gacgatgtgt gctccatgtc acctgtagaa gcggtacgag
     4141 tacagtgatc tactattcgg aacagctatt tagcatggaa acgataggtg caagcaagtt
     4201 aagggttgca tcgcggatgt caatctaggc tataccctaa ggaaggtgcg aacaagtccc
     4261 tgatatgaga tcatgtttgt catctggagc catggaacag ggttcatcat gagtcatcaa
     4321 cttaccttcg ccgacagtga attcagcagt aagcgccgtc agaccagaaa agagattttc
     4381 ttgtcccgca tggagcagat tctgccatgg caaaacatgg tggaagtcat cgagccgttt
     4441 taccccaagg ctggtagtgg ccggcgacct tatccgctgg aaaccatgct acgcattcac
     4501 tgcatgcagc attggtacaa cctgagcgat ggcgcgatgg aagatgctct gtacgaaatc
     4561 gcctccatgc gtctgtttgc ccggttatcc ctggatagcg ccttgccgga ccgcaccacc
     4621 atcatgaatt tccgccacct gctggagcag catcaactgg cccgccaatt gttcaagacc
     4681 atcaatcgct ggctggccga agcaggcgtc atgatgactc aaggcacctt ggtcgatgcc
     4741 accatcattg aggcacccag ctcgaccaag aacaaagagc agcaacgcga tccggagatg
     4801 catcagacca agaaaggcaa tcagtggcac tttggcatga aggcccacat tggtgtcgat
     4861 gccaagagtg gcctgaccca cagcctagtc accaccgcgg ccaacgagca tgacctcaat
     4921 cagctgggta atctgctgca tggagaggag caatttgtct cagccgatgc cggctaccaa
     4981 ggagcgccac agcgcgagga gctggccgag gtggatgtgg actggctgat cgccgagcgt
     5041 cccggcaagg taaaaacctt gaaacagcat ccacgcaaga acaaaacggc catcaccatc
     5101 gaatacatga aagcccgcat ccgggccaaa gtggagcacc cgtttcgcat catcaagcgg
     5161 cagttcggct tcgtgaaagc cagatacaag gggctacaga aaaacgataa ccaactggcg
     5221 atgttattca ccctggccaa cctgtttcgg gtggaccaga tgatacgtca gtgggagaga
     5281 tctcactaaa aaccggaaat aacgccagga atggcgaaga aacggtctaa ataggctgat
     5341 tcaaggcatt tacgggagaa aaaatcggct caaacatgaa gaaatgaaat gactgagtca
     5401 gccgagaaga atttccccgc ttattcgcac cttccccagg tcggacaacg agactctggg
     5461 ttgttgtccg tgcctgtcat tcagtcaagg tgtagtatca tgtaaacatc gtggttttgt
     5521 gaggtgtata tgagtgttat gtccgttcgc ttaccggatg aggtagagca acagttgggg
     5581 caactggccc aatcaacagg aagaaccaaa tcgtggttgg cgaatcaggc tatccaggat
     5641 tatttggcgc gtgaagcctg gcagatcacg gaagttgaag ccgcattgcg tgaagcggat
     5701 gcgggcgact ttgtgcctga aaaggagatg atggcgaagt tcaatcggtg gggtatcaat
     5761 gcaggttaaa tggctgcgta aggctgcact gaaccttgag gatgctcacg atcacttagc
     5821 aaaagagaac tccagggtgg cccaggagtt tgtattagag gtgtatcgct tggttcactt
     5881 gttggtagat cagccagcaa tgggacggcc agggcgcgtt cctggtacac gagagcttgt
     5941 gctgcaaccc tatccgttcc tcattccata ccgagtgaag ggcgaggaga tccaaattct
     6001 gagggtgttt catacccgct tgcggctacc aagccactgg tgaagggtgc ttgtaggtag
     6061 ggcggtatgc gtttgcgtat ggagggggtc gtgcggtaac gtactgccta gaaaagacaa
     6121 accccgaact tctgttgatc aacttggcgg gagattacag agagttcggg gccgtactta
     6181 tgcaataggt gaaatatatc acatggatca caccagacaa ccctttagcg ccgccaatat
     6241 gcgcggtggt cattgaatcg agcacagctt gacctgttcc gggagcgcct gccccggaag
     6301 ccctaccaca ccgacgaatt taccacgggt ctgtccattg cggatgtcag ccgtgccctt
     6361 ggggctcgtt acatccagcc gaacgggcca acgcatcggc attggctcgt gtttgacgta
     6421 gaccatgctg ccgctacgtt atcctgggat gacgtaggcg ccccggcacc gaacatcacg
     6481 gtgacgaaca gggccaacgg ccacgcccac ctgatctacg gactggagac cccgatccgc
     6541 actgccccgg acggcaaggt ggccccactg cggtatgccg ccgccatcga ggccgccctc
     6601 cgggagaaac tgggggcgga tctgggctac tccgggctga tctgcaagaa ccccctgcat
     6661 acccactggc ttgtccaggt atgggagccc gcctcctacg atctgagctg gctggccgac
     6721 tacctcgatt taagccccta caatggccgc aaacagcttc cagcttacgg gttggggcgt
     6781 aactgcacct tgttcgagaa aacgcgccag tgggcttaca aggcgattag gcaggggtgg
     6841 ccggggtatg aggcttggct ctctgccgtt atagatcggg cgatcgggta caacgtccag
     6901 ttcgagccgc cgctcccggc caacgaggtc aggcacatcg ctaagtccat tgcgaagtgg
     6961 acgcaccgta acctaacccc cgcagggttc agcgccgtac aggccgccag aggggccaag
     7021 ggtgggaaag tatccaaggg tgggggcagg cccagtaata gcggtaaggc tcgtgcagac
     7081 ctcttaccgg aggtgctcag gcttaagggt atggggtaca gcaaccgcga cataggtgag
     7141 gatcttggga tctcgtccag tacggtcagc ctgtacctga aacgcgaatc tgagtgatcg
     7201 aaacaagcct tatcagatag tagcccccgg agggggttct ttgagccctt aaaatcattc
     7261 aagagagagg attggtgcga ggggggtaag gccgatcccg aagggctgtc catcaggcca
     7321 tgtgtttggc ctgtgggcag taggtcttat ccccggagct gtttgttctc tgccgttcgc
     7381 agtgcccgca ggatctcccc ggcctgctcc ggggtcagta ccaccgccga ccctccatcc
     7441 cggataacct gcatctgggc atccagcccc tctaccaatg cccgatttat cgtcacagta
     7501 acgttattgt cctgctgctt tgcacggtag gctgcttgtc gttctgcccc tgttttggct
     7561 gatccggttt ttggcctacc ccgtccgcgc ttgatgccaa acatgtcccc agtcccctga
     7621 tccttggtgt ccttcatggt gttatttcct gtcactgtaa cggtaattga ttatgtgtca
     7681 ctgtgacgat aattggcaag ttgccgaccc cctgtttttt tgctgtactg gtattaagcc
     7741 agccgacccc gtattacgtt ttcgcccgct tcgcggctca acgtaaacgg gggctggtga
     7801 ggggctccgc cccttcaaac cctgcgccgc tgcgctgctt ggagagccaa tgacaaagcc
     7861 agagctaaag acaacaacgg ccagccttcg cctcgatcct gccaccctag ccaagtggcg
     7921 caagggtgcc tgtgacgagg gcctgaccct ctccgactgg ctgagagcga aggtggatgc
     7981 cgaccagcaa acccacattg cccccgcccg gtcgcggcgg gaacgagacc cccgccacct
     8041 ggcagatccg gcgctaatgc gccagctcgc ggcgttgggg tcgaacctca accagatcgc
     8101 cagggcggtg aacgaatgcc gcctggtggg gagcccggtt tacctggtgg agctgctggc
     8161 gttgctacgc agcatcgagg aggcagccgg aacgttgctg ccgcagcttc cgccgccgcc
     8221 gaaccgggag ggtgacgggc atgatgatta agttcctggc tcatggcacc ggcaagggca
     8281 gtagtgcggc gacctatttg ctggacagtc acgaccacaa gggcgaggaa cgggccgggg
     8341 tggcggtgct gcggggtgat ccacagcagt tcgccgccgt ggcggacgcg ctcgattttc
     8401 agcatcgata ccggagtgcg gtgatctcct ggtcggagga agaggccccc agtgacgcag
     8461 agatcggcgc ggtgctggac gacttcgagg cgctgtcctt tgccggactg gataggcagg
     8521 acgtacacct gaccgccgtg c
//
