LOCUS       NZ_AP024319            20722 bp    DNA     circular CON 23-SEP-2023
DEFINITION  Staphylococcus aureus strain S36 plasmid pS36, complete sequence.
ACCESSION   NZ_AP024319
VERSION     NZ_AP024319.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00269945
            Assembly: GCF_019669385.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1
  AUTHORS   Nakaminami,H., Kim,E.S., Kaneko,H., Yokomori,S., Moon,S.M.,
            Song,K.H., Jung,J., Park,J.S. and Kim,H.B.
  TITLE     Comparative genomics analysis for a human variant of CC398 (ST1232)
            methicillin-resistant Staphylococcus aureus isolated in Korea and
            Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 20722)
  AUTHORS   Kaneko,H. and Nakaminami,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JAN-2021) Contact:Hiroshi Kaneko Tokyo University of
            Pharmacy and Life Sciences, Department of Microbiology; Horinouchi
            1432-1, Hachioji, Tokyo 192-0392, Japan URL
            :https://www.toyaku.ac.jp/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Coverage                   :: 2460x
            Sequencing Technology             :: MGI DNBSEQ; ONT GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/23/2023 03:59:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,753
            CDSs (total)                      :: 2,671
            Genes (coding)                    :: 2,593
            CDSs (with protein)               :: 2,593
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 41 of 78
            Pseudo Genes (incomplete)         :: 35 of 78
            Pseudo Genes (internal stop)      :: 24 of 78
            Pseudo Genes (multiple problems)  :: 18 of 78
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP024319.1.
            Annotated at DFAST https://dfast.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..20722
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="S36"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pS36"
                     /geo_loc_name="Japan"
                     /collection_date="2019"
                     /genotype="ST1232"
     gene            166..842
                     /locus_tag="K5606_RS13545"
                     /pseudo=""
     CDS             166..842
                     /locus_tag="K5606_RS13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /name="replication initiator protein A"
     gene            <853..1135
                     /locus_tag="K5606_RS13790"
                     /pseudo=""
     CDS             <853..1135
                     /locus_tag="K5606_RS13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /name="protein rep"
     gene            2347..2964
                     /gene="cadD"
                     /locus_tag="K5606_RS13550"
                     /old_locus_tag="S36TP_25670"
     ARG             2348..2961
                     /name="cadD"
                     /gene="cadD"
                     /gene_name="cadmium resistance transporter CadD"
                     /tool="amrfinderplus"
                     /drug_class="CADMIUM"
                     /identity="99.51"
                     /coverage="100.0"
     gene            2983..3330
                     /locus_tag="K5606_RS13555"
                     /old_locus_tag="S36TP_25680"
     CDS             2983..3330
                     /locus_tag="K5606_RS13555"
                     /old_locus_tag="S36TP_25680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_000119691.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKKL
                     KIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDDEI
                     REFFSKNQEGF"
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            3490..3582
                     /locus_tag="K5606_RS13560"
     CDS             3490..3582
                     /locus_tag="K5606_RS13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_001791613.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            3794..4768
                     /locus_tag="K5606_RS13565"
                     /old_locus_tag="S36TP_25690"
     CDS             3794..4768
                     /locus_tag="K5606_RS13565"
                     /old_locus_tag="S36TP_25690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="WP_000072391.1"
                     /translation="MSNTKIKYQIFISSTYLDLIEERQAVVEAVLNSNNIPAGMELFNS
                     SSKSQWEIITQWINESDAVILLLGSSYGTIDSEENISYTEMEYNFAKSLGKPIYVFEMN
                     KASTESAEKSKKLDEFKTKVKENRLISPNINDISELKSKVSQTLMQSVPSEIKENSGWI
                     RVQDCGKNVKKLKKLDAIQRDLDTYDFLKEELYKIFMYVKTFYFQHTPFDDSYIEDIEN
                     YLLNYENNPHKSFMKIKVYDYKNFLKHLKNILILIKQGKKPLAQAGHFKLSFYEEDLKL
                     LQKNYKTTLVDIKGLNKHFYKECDRCLKAYNIFIEGINKHLYE"
                     /name="DUF4062 domain-containing protein"
     gene            complement(4888..5703)
                     /locus_tag="K5606_RS13570"
                     /old_locus_tag="S36TP_25700"
     CDS             complement(4888..5703)
                     /locus_tag="K5606_RS13570"
                     /old_locus_tag="S36TP_25700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467708.1"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_000164958.1"
                     /translation="MTKNQNFIETKEYKRFAEFCDACIKYQYIGICYGQPGVGKTLSSR
                     YYTNWDTIEKQVNHRGWEDLASKTTDDILSVDKIFYNAPAEKQTKLRNDLYSITAIIDL
                     GQKLHVVNKYGHEHSKHYSDMFKYIDLIIVDEIDRLKVQHLEQLRAIYDEHNLAMIFIG
                     MPGIEKKLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWQDLGFDLKLEDFTDYEAIT
                     TIIKITKGNFRLIHRLFAQIDRIMDINGLDKISTEVVETARDSLVIGIR"
                     /name="AAA family ATPase"
     gene            complement(5696..7139)
                     /locus_tag="K5606_RS13575"
                     /pseudo=""
     CDS             complement(5696..7139)
                     /locus_tag="K5606_RS13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790716.1"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /name="DDE-type integrase/transposase/recombinase"
     gene            complement(7111..7704)
                     /locus_tag="K5606_RS13580"
                     /old_locus_tag="S36TP_25730"
     CDS             complement(7111..7704)
                     /locus_tag="K5606_RS13580"
                     /old_locus_tag="S36TP_25730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690636.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000690638.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPGL
                     DKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSSTGQ
                     LLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNQKDLNLLKTLYDNGTPI
                     KTIAEQWHVSRTTIYRYLNKLEDKEDEKQGEVSN"
                     /name="recombinase family protein"
     gene            complement(7968..8348)
                     /gene="blaI"
                     /locus_tag="K5606_RS13585"
                     /old_locus_tag="S36TP_25740"
     ARG             complement(7972..8348)
                     /name="blaI"
                     /gene="blaI"
                     /gene_name="penicillinase repressor BlaI"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="99.21"
                     /coverage="100.0"
     gene            complement(8338..10095)
                     /gene="blaR1"
                     /locus_tag="K5606_RS13590"
                     /old_locus_tag="S36TP_25750"
     ARG             complement(8342..10095)
                     /name="blaR1"
                     /gene="blaR1"
                     /gene_name="beta-lactam sensor/signal transducer BlaR1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="95.56"
                     /coverage="100.0"
     gene            10202..11047
                     /gene="blaZ"
                     /locus_tag="K5606_RS13595"
                     /old_locus_tag="S36TP_25760"
     ARG             10203..11044
                     /name="blaZ"
                     /gene="blaZ"
                     /gene_name="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            11416..12024
                     /locus_tag="K5606_RS13600"
                     /old_locus_tag="S36TP_25770"
     CDS             11416..12024
                     /locus_tag="K5606_RS13600"
                     /old_locus_tag="S36TP_25770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436987.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001566953.1"
                     /translation="MIVGYARVSSNDQNLERQLENLKTFCAEKIFTEKQSGKSIENRPV
                     LQEALNFLRMGDRFVVESIDCLGRNYDEVIHTVNYLKDKEVQLMITSLPMMNEVVGNPL
                     LDKFMKDLIIQILAMVSEQERNESKRRQAQGIQVAKEKGVYKGRPLLYSPNAKDPQKRI
                     IYHRVVEMLEEGQSISKIAKEVNITRQTIYRIKNDNDLI"
                     /name="recombinase family protein"
     gene            12438..12731
                     /locus_tag="K5606_RS13605"
                     /old_locus_tag="S36TP_25780"
     CDS             12438..12731
                     /locus_tag="K5606_RS13605"
                     /old_locus_tag="S36TP_25780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000726503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_000726502.1"
                     /translation="MKKKFVSSCIASTILFGTLLGVTYKAEAATVHVAGGVWSHGIGKH
                     YVWSYYSHNKRNHGSTAVGKYSSFSGVARPGVQSKASAPKAWGGNKTFYSLH"
                     /name="lactococcin 972 family bacteriocin"
     gene            12806..14740
                     /locus_tag="K5606_RS13610"
                     /old_locus_tag="S36TP_25790"
     CDS             12806..14740
                     /locus_tag="K5606_RS13610"
                     /old_locus_tag="S36TP_25790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="WP_000713729.1"
                     /translation="MKKFKLFLDFSTLLLISGLLFLFFFKENEEIIPESSNILTISNWD
                     KSNSKSKVLDVIESGAKNQNIQIIKSVKDFDNKKEFFVFNSKRNNSDFIRNKTSLLTPS
                     DLLNREIKGKYYIIGEHFNVEELTSELKKAGLTSEVEYINRNILFFELVIDNDLLVPFI
                     FLGVLYLLYFLYDRGYNLKFYAIQKLHGFSTTRIIFHNIHIKIIYWLVLTAIFFIANIL
                     IIHIANLELDFLMFIRRFIVLLFVFACITTLLWLISYSLLLKLSIPLILKGKKGYKFSI
                     FMGTFTKCIMVLILSFLLAQNLNAYTKLKNIYDSEKIWQKLDNYYTLEISPNIRNSEEK
                     QILETNIYNLIKKSEQLGGLLLKNNNIANPDKNNYIPENGNVFFINNNFLNFYKNINHD
                     FKMIDNHSDKINVFIPPRLQYQKSEIQKNHQGWIDFQKQQNKKVDVQVLSKNVSVFSFD
                     RTTNLKFGYLDSPIVMLLTLDDLTGDFYLAAVSQGGYLFKDLDTLKQLLKDNHLEEEIS
                     GITNYKDSVLNELNETKTKMIITIVTIIINVFILLIATVFETIQYFSWNKKQLLLRKIH
                     GYSLFSSNVRYLTISILLSIMLAYTTHILFGSKILLFIIMSIAIIQHLLQIAYIKYLEK
                     YFKDLMREI"
                     /name="bacteriocin-associated integral membrane family
                     protein"
     gene            14744..15055
                     /locus_tag="K5606_RS13615"
                     /old_locus_tag="S36TP_25800"
     CDS             14744..15055
                     /locus_tag="K5606_RS13615"
                     /old_locus_tag="S36TP_25800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="WP_000831610.1"
                     /translation="MKRTLIIVTIVLLIIILSLLLVRNEHLDRFNPLLKEKVSYAKVPK
                     DTQDYRNITVYSKNGEKKSYKLDFLGYDPTKEYVKVNHKGKYVRSIEYITKDIPSFLK"
                     /name="YxeA family protein"
     gene            15067..15693
                     /locus_tag="K5606_RS13620"
                     /old_locus_tag="S36TP_25810"
     CDS             15067..15693
                     /locus_tag="K5606_RS13620"
                     /old_locus_tag="S36TP_25810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_000593103.1"
                     /translation="MIKLENVFVKKGNKNILDGCNFNFEKGKSYALVGESGAGKSTLLN
                     IIAGFEDVSQGSIYIEDKLLKKKVDFYRYTLGYLFQNFALLENQTISQNLDLALKFKKN
                     KKDNMNLKKEVLKKVGLDLDIKRIVSSLSGGEQQRVALARLILKDPKIILADEPTGSLD
                     TKNGKIVIDLLLKLLDENKTMIVVTHDLELAKRFDVIVNISELRS"
                     /name="ABC transporter ATP-binding protein"
     gene            15931..16560
                     /gene="mobV"
                     /locus_tag="K5606_RS13795"
                     /pseudo=""
     CDS             15931..16560
                     /gene="mobV"
                     /locus_tag="K5606_RS13795"
                     /inference="COORDINATES: protein motif:HMM:NF013258.3"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /name="mobV"
     gene            16654..17199
                     /locus_tag="K5606_RS13635"
                     /old_locus_tag="S36TP_25840"
     CDS             16654..17199
                     /locus_tag="K5606_RS13635"
                     /old_locus_tag="S36TP_25840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001805533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000273005.1"
                     /translation="MYEIKTKNVGGWFHKEKQETGNIVITKTDFEKYTKQIKAAQMILD
                     DYECIKSGKSLKKAEKQNESLVNELTSVHMENEKLVEEFNDLAQRYNYLLSENEKKDKE
                     LNYTLKLFNQVFKIIKSMMKEERYHTLINHIDNHLDNSKIREVMTIDNNDEQFFKKKYQ
                     AQEREIIFKEDREDGYTL"
                     /name="hypothetical protein"
     gene            17583..18443
                     /locus_tag="K5606_RS13640"
                     /old_locus_tag="S36TP_25850"
     CDS             17583..18443
                     /locus_tag="K5606_RS13640"
                     /old_locus_tag="S36TP_25850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_000145511.1"
                     /translation="MTGETVVYKNEMNLVPLRRFTATEINLFFAMCNKLKEQDTNTLRL
                     SFDELKKLSNYSPETRNINRFANDLDNVYKKMLNLTIRYEDDDVIERFVLFNHYRIHKR
                     EQYLEISTSSNLKHILNSITNNFTKFELKEMTRLKSTYSKNMFRLLKQYKHTGYLKIHI
                     DDFKNRLDIPKSYRMTDINKNVFKPIIIELGSIFNNLTINKIKAKKGRKIEWIEFTFDA
                     EKRIHNKRQPQMSKIDKSRQYVRREKTPKWLEERSYEKQPQKDYDPQLEKEREDFLKQL
                     ELNWE"
                     /name="replication initiation protein"
     gene            18530..18871
                     /locus_tag="K5606_RS13645"
                     /old_locus_tag="S36TP_25860"
     CDS             18530..18871
                     /locus_tag="K5606_RS13645"
                     /old_locus_tag="S36TP_25860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761340.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_000761341.1"
                     /translation="MKLAEAIKEQRELKGWSQEELANILKVSRQSVSKWESAKNYPSLD
                     ILIAMSDLFGISLEHLIKGDARFKKVILEGNYRESNRVPSIVDFLYDFWWLFFPVAGFL
                     VWAIHSFMG"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(19110..19181)
                     /locus_tag="K5606_RS13650"
     CDS             complement(19110..19181)
                     /locus_tag="K5606_RS13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_106873653.1"
                     /translation="MNEILVHIMTTAISGCLVTLFGY"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            19330..19515
                     /locus_tag="K5606_RS13655"
                     /old_locus_tag="S36TP_25870"
     CDS             19330..19515
                     /locus_tag="K5606_RS13655"
                     /old_locus_tag="S36TP_25870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000466619.1"
                     /translation="MFGDQRQEATKYVIKEGYQDIYFLNKNGEWYYFEVRSVWRGKHII
                     RVKDGLLGWRKEIVTE"
                     /name="hypothetical protein"
     gene            complement(19734..20477)
                     /locus_tag="K5606_RS13660"
                     /old_locus_tag="S36TP_25880"
     CDS             complement(19734..20477)
                     /locus_tag="K5606_RS13660"
                     /old_locus_tag="S36TP_25880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_000843758.1"
                     /translation="MKSVKRLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDIE
                     KIIQRVNENKKKYGFESATEDKQKKESDNINFESKSDAQIVEILKNQINTLNNQIEKQE
                     SRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTSTNAR
                     QNVDAQEKTYTTSPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAQREDVSANPND
                     NDSDIEEKSEETEPKKGFWSRLFGN"
                     /name="DUF536 domain-containing protein"
     ARG             10203..11047
                     /name="PC1 beta-lactamase (blaZ)"
                     /gene="PC1 beta-lactamase (blaZ)"
                     /gene_name="BlaZ beta-lactamase"
                     /tool="rgi"
                     /drug_class="penam"
                     /identity="96.8"
                     /coverage="100.0"
     BGC             7438..17731
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          1193..1340
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="81.333"
                     /coverage="100.0"
                     /evalue="2.96e-25"
     Typing          17584..18443
                     /name="rep_cluster_2214"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(19735..20477)
                     /name="rep_cluster_1733"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.866"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          15932..16206
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.565"
                     /coverage="100.0"
                     /evalue="4.71e-46"
ORIGIN
        1 ttggtaataa aacgtttaga aactgtttat ttatataatt ttcgatactt agattatata
       61 atgaataata tcttagtgca agcacctaga ttattaaata aacacaaaaa atgtaagcgt
      121 tttattacct cttaccaaac taaaattcgg aaagaggttt ttattatgtc aattaaactt
      181 tacaacataa atgaggaata cagagaaaag ttttatcaaa taccgaaagt attttttaca
      241 aatcctaaat atatcaagtt atccaatgat gcaaaaatgg catggggtat tcttagagat
      301 cgtttagatt tatcaattaa aaatggttgg gttgattcta aaacaggtaa tatttatttt
      361 tattacaaaa atgaaaaccc acaaaatata ttaaattgta aaaaagataa agtaattaaa
      421 ataaagaaag aacttaattg aagccgaatt actactacaa gaaagacaag gtttaaataa
      481 gacaaataaa ctttatttag ttaaaccaga agtaacagaa gatgatattt ataaaattca
      541 aaagcaagaa aacactgttg agagcagcaa tggcaaggaa gtcggaaaaa ccgacttcaa
      601 gaagtcgata aaacagaatc taatgatact gaacttagct atactgaatc taacaatact
      661 gaaataaatg atatgaatga cttgaatgat atagagtccg aaaatgaaat tagtaatcat
      721 tcaagtcaat gcaaggttat ttaatgcaaa caattttaac tgaatttgaa gaaacacatg
      781 cactttatat gcgccgtaaa aacatgaaac aatataatat ctttaatcaa tcattcaaat
      841 gattttataa caatcaaaga tacatcaaaa tatttcgtta aatcatctga ctttttaact
      901 gatagcgatg aaaaaatcaa gaaattgtca gtgatctaga aaaagatttg tatcgaaaac
      961 gtatgttaag ttatggtgga ttgcttaaat aaaaacataa aattttaaac ttagacgatg
     1021 ctgaagatgg caatttaatt aatacaagtg atgaagataa aatagcaaat gaagaagaaa
     1081 aggcacattc gattacggca attttgaaaa gcaaaattat tatttaaggc attagtgata
     1141 actgatgtct ttttttatta cttttttgta aaaaaagtgc tgtcttattt ttgtgacaaa
     1201 tgctgtatgt agtgtcacaa aagtgtgaca cttgagcttt ttatgacccc acacaaaaac
     1261 attttcgaag ccttgaaata tctggctttg ccagatcccc cattcattga tggggacaaa
     1321 tttcccttat gctcttacgg agattttaga gttaaattaa aatttctaag tttttgataa
     1381 gaaaagccct gcaggaattt agaagaacgt ggggaagttt taaagaattt tatgctaatt
     1441 tttaatttgc atgtaaatgg gcagtgtcta aaaaatagtt attgtttttg ctcaaatttt
     1501 tgtttttgaa aatagcatat atttatttgg ctcatatttg cgttttaaaa gcgtatataa
     1561 gttttagata taaaactata tgaatcaccc ctaaatcttt aaaatgccct cttaaaattc
     1621 aaaataaggg cataaaaaat aaaagccccc cccagataaa gtgaggtagc tttttctttc
     1681 gacgccgtat cgtcgatgta attcaaaaaa gttatgggct ataaatctac atcacttttt
     1741 caagaatgta gtagcaatat tcaacgcaaa ttaattttta taactgctcg gaatatctca
     1801 agccgtttct ttaattttga aataaaaaaa tcgacgaagg tcgattacgt ttttgcacat
     1861 gtcgatttat ccgacgtata agtgcgccct tacgggattt aactagatta taacgacgaa
     1921 ttttagacct gtaaagcaat aacaattagg tctaccaggt agataaagtg tacaaagtag
     1981 aaccgaaaaa gttatttatt ttgaagattt ttcgtagata taatttgcta ataaacttcc
     2041 taaaactgaa taaagaaaaa tagttaaact catgtaatca ctccataatt ctgaagtgtc
     2101 tccatgtcta cgcatagaca cgcatttatg ccgagaaaat ttattgtgcg ttgagaagaa
     2161 cccttaacta aacttgcaga cgaatgtcgg catagcgtga gctattaagc cgaccattcg
     2221 acaagttttg ggattgttaa gggttccgag gctcaacgtc aataaagcaa ttggaataaa
     2281 gcacctatga ttatttaaaa atataagtta taatacattc aaaaatattt ttgaatgagg
     2341 tgtattatga ttcaaacggt tgttgctgct gctgttcttt atattgctac agcagttgat
     2401 ttattagtaa ttttattaat attttttgct agagcaaaga ctagaaaaga atatagaaat
     2461 atttatgttg gtcaatattt agggtctatt attttaatat tagttagttt gtttttagct
     2521 tttgtattaa attatgttcc agaaaagtgg atattaggtt tattgggttt aataccaatt
     2581 tatcttggaa ttaaagtggc tatttatgat gattgtgaag gagaaaagag agctaaaaaa
     2641 gaattgaatg aaaaaggatt gtctaaatta gttggtacgg ttgcaattgt tacgatagca
     2701 agttgtggcg ccgataatat tggtttattt gttccgtatt ttgtgacatt aagtgttact
     2761 aatttattac ttactctgtt tgtcttttta attttaattt tcttcttggt gtttactgca
     2821 caaaaattag ctaatatccc aggaattgga gaaattgttg agaaatttag tcgttggatt
     2881 atggctatca tttatatagc tttaggttta tttattatta ttgaaaatga cactattcaa
     2941 acaattttag gatttatatt ttaatttagg gtgtgatttt atatgagtta tgaaaatact
     3001 tgtgatgtga tctgtgtaca tgaggataaa gttaacaatg ctttaagttt tttagaagat
     3061 gataaatcta agaaattact taacatttta gaaaaaattt gtgatgagaa gaaattgaaa
     3121 atcatattat ctttgattaa agaagatgag ttgtgtgttt gtgatatttc tttgatattg
     3181 aaaatgagtg ttgcttcaac ttcacatcat ttaaggcttt tatataaaaa tgaggtactt
     3241 gatttttata aagatggaaa gatggcatat tattttatta aagacgatga aataagagag
     3301 tttttctcta aaaatcagga gggtttttga aacgagtttc ttcttgtctt gatactataa
     3361 aaaacaaagt tatataacat aagacgactt atcggaagtt atctatcatc gtatattcgg
     3421 agatgatata atatacaggt cgcctatctc tcaggcgtca attcagacgc agagaggagg
     3481 tgcattactt tgctaatcat tttcgttcac atcatagcac cagtcatcag tggctgtgct
     3541 gttgcgtatt atacttattg gcttagtaaa cgcaacaaat aattaatagg cgactttata
     3601 agccagcatc gaaaaaatcc ccacactatt gcagtagtgt ggggattggt gcattaattg
     3661 ttaatcattt tcgttatcta gattataaca ctggttagta gaggaatgca aaaagataat
     3721 attttaagta gtctttttgg atattatcat atgatatatt tatgtaaatt gattttttat
     3781 taaaggagca tatatgagta atactaaaat taaatatcaa atttttattt catcaactta
     3841 tttagattta atagaagagc gtcaagcagt agtagaagca gttttaaaca gtaataatat
     3901 tccagcaggt atggaacttt ttaactcaag tagtaaaagc caatgggaaa taataacaca
     3961 atggatcaat gaatcagatg cagtgatatt attgttaggt tctagttatg gaacaattga
     4021 tagtgaagaa aatattagct atacagaaat ggagtacaat tttgctaaat ctttaggtaa
     4081 acctatatat gtttttgaga tgaataaagc atctaccgaa tccgcggaaa agtctaaaaa
     4141 attagatgag tttaagacaa aagttaaaga aaatagacta attagtccaa atattaatga
     4201 tatttcggaa ttaaaaagta aagtatctca gactttaatg caaagtgtac ctagtgaaat
     4261 taaagaaaat agtggatgga taagagtaca ggattgtggt aaaaatgtaa aaaaattaaa
     4321 aaaattggat gctatacaaa gagatttaga tacatatgat tttttgaaag aagaacttta
     4381 taaaatattc atgtatgtaa aaacttttta tttccagcac acaccatttg atgatagtta
     4441 tattgaagat atagaaaact acctgctaaa ttatgaaaat aatccacata aatcattcat
     4501 gaaaataaaa gtttatgatt ataaaaactt tttaaaacat ttaaaaaaca tattgatttt
     4561 aattaaacaa ggaaaaaagc cattagctca agccggtcat tttaaattaa gcttttacga
     4621 agaggattta aaattgttac aaaaaaatta taagactacg ctagtcgata ttaaaggatt
     4681 gaataaacat ttttataagg aatgtgatag gtgcttaaaa gcatataata tttttattga
     4741 gggtataaat aaacatttat atgaatgata taagtacacc ctaatcatac atatataatt
     4801 gttgtcactt tactcgatat atgagaatga ctttacctaa taaatgagaa aagcaacgca
     4861 ctatattaaa atgcgttgct ttttctatta tcgaatacct ataactaaac tatctctagc
     4921 tgtttctaca acttctgtac ttattttatc taaaccattt atatccataa ttctgtctat
     4981 ctgcgcaaat aaacgatgaa tcagcctaaa attccctttt gtaattttaa taatcgtcgt
     5041 tattgcctca taatcagtaa agtcttcgag ttttaaatca aatcctaagt cttgccactt
     5101 atattctaat atatgatgcg tctcatcttt acttagatta tcaaattcat gcgcaaaacc
     5161 tatacgcgaa tatagttgag gataacgaga tagttttttc tctatacccg gcataccaat
     5221 aaatatcatt gctaaattat gttcatcata aatagctctt aattgctcta aatgttgcac
     5281 tttaaggcga tcaatctcat ctactataat taaatcaata tatttaaaca tatcactata
     5341 atgtttacta tgttcatgtc catacttatt tacaacatgt aatttttgac ccaaatcaat
     5401 gattgcagta atactatata aatcattcct taatttagtt tgtttttctg ctggtgcatt
     5461 ataaaatatt ttatccaccg atagtatgtc atcggtcgtt ttactagcta aatcttccca
     5521 acctctatgg ttaacttgtt tttcaatagt atcccaattt gtgtaatacc tagacgataa
     5581 agtttttccg acaccaggtt gaccataaca tataccaata tactgatatt taatacaagc
     5641 atcacaaaat tcagcaaagc gtttatactc tttcgtttca atgaaatttt gatttttagt
     5701 cattacgata tctctttaat tttttgacgt ttttagtcgt ttcttgaggt gaataaccac
     5761 aatttttttc ttccttaatt aattccgtgg tacttggact agcaatattt tgttttaagt
     5821 gttttcttct ctgactacga gcgtgctgta tgtcttttat atctatggag taatcagcta
     5881 tttctggaca tatagcagta caaagaaatt catctctata aaacacacgt atttcagcca
     5941 tatcatttgg gttataacga atcagtacat attcacctac ataagcttct agattagtat
     6001 tactgtatcg aaaaccttga aaatgaatgc catctgaatg aacttttcta gatttaggta
     6061 tttccaacaa taataaatca agttgttcta agctacttgg catattaggg aaaaagtgat
     6121 tactattcca atgactgata ggcgtggtat gaatagtact atgttctttt tgattataat
     6181 cttcaattaa aaagtaacgt aatttttctt caaaattgtg aaaatttatt agattactag
     6241 gagtatcatt attgtttata tatccaggca gttgttctaa gaaagtttga ttaactgtct
     6301 gaaagaatcg ttctattttt ccacgaccac gaggaacacc aactttcgaa aacatcaagt
     6361 taattttcaa atcaatagcg acttgttcca tatgatgtga agtaaagtca cttccatgat
     6421 ctgtgtagaa tttttcgggt ataccacaca ctggccaatt ggtatcattt ttattccata
     6481 ttgcttggtg tagtgttaat gctgtgtttt gagcattggg gggcatcaaa acttaagaaa
     6541 tagcctgcaa ttgcacgact atagtcatcc ataataatag ttagccatgg tctattgata
     6601 ttaccttttt ggtctaatat aaagatatct agcaaagtat ggtctgcttg ccatatttca
     6661 ttgggtctcg aggattctct aatttgtatt aaatcgtact tattttgata gtatttttca
     6721 ccctgatgag agaaatcaat aacagactta ggcatcgctt taataacact ataaatttgt
     6781 ttataacttg gtatatcaaa attattttct ttacagtaat tagtgatttt cctatgaatt
     6841 gtggctatac tatttctttt attcataagc gcaaggcgtt caatttcatc aattatcttt
     6901 ggttctattt tacgtgttcc tttatcagct cttgtcgcat agattagtcc tttaagtcct
     6961 tgttctttat atgacttatt ccacctataa agggtgctta acgcaatccc tttactttta
     7021 gaaatacttg ataaagattg ttcgcctagg ataaaaggtt ttataatatt gtacttttga
     7081 gtagcttttt tacgtttggc ttcagaaaaa ttagttagat acttctcctt gtttttcatc
     7141 ttccttgtcc tccaatttat tgaggtaacg ataaattgtt gtacgtgaaa catgccactg
     7201 ttctgcaatt gtcttaattg gcgtgccatt atcataaagg gtcttaagta agtttaaatc
     7261 tttttgattt aatttttctg gtctgcctcc atatcggcct ctagcacgtg ctgcaattcg
     7321 gccagctgaa gaacgctcta aaattaaatt acgttcaaat tctgcaaatg ctgcgaataa
     7381 atgaaataat aattgtccag ttgaacttga tttatccatt gttatatttt cttctaaact
     7441 atggaaactt acacctcgtt cgttaagctc attaactaat gtgattaaat cctccatatt
     7501 acgtcccaat ctatctaatc tccaaacaac aatcgtatct cctgacctcg caaattcaat
     7561 tgctttatct aaaccaggtc ttttactttt tgaaccactc atatggtcat taaatatctt
     7621 ttcacaacca tatgtattta atcgatcctc ctgtaaattt aaattttgta atccagttga
     7681 aactcttgca taacctattt tcaaaataca aacctcctat atacatcttc agtgtaccat
     7741 aacttaatta caaggtcttt tatgaacata gattttttgt tgggtttttg aacacataat
     7801 cctatgtaat tcttacttat ttagacataa aagaagagtg tacagaaaat ggttattttc
     7861 tgtacactct catctttaat aatatatttt aaatgtcatt aacaaaacta atttaataag
     7921 agtcaagcat agtttacata tgtattatgt ttgaaacata cctctaatta ctttttacta
     7981 atatcattta aaatatctcg caattcttca atttctttgt tatttaattc ttcatttttc
     8041 gcaaaattca acactaaact tttcatatcc cctccataca gtttattaag aaaggtttta
     8101 gcagttttca ttttaatatc gtcttcttta atatttgatg agtaaaaata aatattttct
     8161 gatttgtatc gttttataat ctctttttta tatagtcttg tgattaatgt tctaatcgtt
     8221 ttatcactaa cttctttata tttttgaatt tcaactacaa tttcattagc tgatactgat
     8281 tttttatccc atattatatt cataacatcc cattcagcca tagatatttc aacttgctta
     8341 ttggccattt aaaacaccca ttccttttaa tattttttca cttattaatt cagcattttt
     8401 cccagacggg tttccatctg ataaatgtgt agcaaaataa tacttatcat gatttgtaat
     8461 tacgtaacct acaaaccacc cattattata cttcccgttt actatacctg tacctgtttt
     8521 cccatacagt tcatactttt catttttctt aatcaataat gaagaagata attgattctt
     8581 tgctttttta ctaaaatggt tattttgttc catcatattt ttaaaaacta ttacttgttc
     8641 aagattagat attttcaaac tatcttccat ccaatagctt ttataacttc ccaaattttc
     8701 attaccataa tttaattgct tgagttgagc cgcagtatag ttctttggta tttgattact
     8761 aatacgttcg aagtaccaat taactgaatt ttgcattgct gtatttaaat cttgttcctt
     8821 attccaagac tcgaaaagat aatgtttatg attccatgac atacgtgaat ttttatcact
     8881 tataatatga cggtcaagtc caaacagtgc taaataaatt ttgtaagttg aatcaggaga
     8941 atatcttttt ctactttctt tttcattata aatatagtat ttgtctttct tcatgctgta
     9001 cataacgaaa gatccactat ttgaaccaaa atttttactt tcatctaaaa tttggtaatc
     9061 gctttgtaag ggctttttat aattataatc agttaaggat tgccccatta aaaactggct
     9121 ttgaattatc atgataaaaa aagtaaaaat acaaataaaa attagaatta attttgactg
     9181 ctttttcaga tcggcttctt ttatattaat taatctattt ttgagtaatg attttttacc
     9241 attaaatgaa tggcttaata tatttttgtt gaaaaatgag gtttttaata cggagtccat
     9301 aatagattcc gcataagttt taaattcatt tttgttaata ttattaagga caaatctatc
     9361 ggcttctact tcattgtcat gaattatctt tctttttaca atatgtacta atggattgta
     9421 actcataata atagagaaga tattaaagat aattaaatgt aaagtatctc tatttttagc
     9481 atgagcatat tcatgcagta ttatatattt tagtctttta tcaattacac ttttaaaata
     9541 tgaactagga atcaaaataa tatatttccc ataccaaaaa gttattggag attgaatagc
     9601 ctctgctttt cgaatcacaa tatttttttt atattgatgg ttgaaaagta tcgtatctat
     9661 tttatttttt tcattttcgt ttagataaag tgactgtttc tttaaatatt taagatataa
     9721 taaggctttc aaaaatttaa aacttaaaat aataactaaa actatccaaa taactgtgca
     9781 gatattatca attgaatccc aattaaactt atggatatct gttgcgaact cttgaacagg
     9841 tttggtggta attatgttat ggttcaagtc gtgtgactta ctttcaactg tgggcgcttg
     9901 attattcaca ttattaaatt ttataaaaga gaatttaatt gggataaaag gaattaatcc
     9961 agcaagcaca gttagatacc aaactttata atttaaacta taattaaaat agcgttttaa
    10021 tatgtaccta aaaaatacta acaatagaaa tataaaacaa aagcttacta cactcattat
    10081 tagtaattta gccattttga caccttcttt caaatattta taataaacaa ttgacatcaa
    10141 tattacaatt gtaatattat tgttttgtaa aaattacaac tgtaatatcg gagggtttat
    10201 tttgaaaaag ttaatatttt taattgtaat ggctttagtt ttaagtgcat gtaattcaaa
    10261 cagttcacat gccaaagagt taaatgattt agaaaaaaaa tataatgctc atattggtgt
    10321 ttatgcttta gatactaaaa gtggtaagga agtaaaattt aattcagata agagatttgc
    10381 ctatgcttcg acttcaaaag cgataaatag tgctattttg ttagaacaag taccttataa
    10441 taagttaaat aaaaaagtac atattaacaa agatgatata gttgcttatt ctcctatttt
    10501 agaaaaatat gtaggaaaag acatcacttt aaaagaactt attgaggctt caatgacata
    10561 tagtgataat acagcaaaca ataaaattat aaaagaaatc ggtggaatca aaaaagttaa
    10621 acaacgtcta aaagaactag gagataaagt aacaaatcca gttagatatg agatagaatt
    10681 aaattactat tcgccaaaga gcaaaaaaga tacttcaacg cctgctgctt tcggcaagac
    10741 tttaaataaa cttatcgcaa atggaaaatt aagcaaaaaa aataaaaatt tcttacttga
    10801 tttaatgttt aataataaaa acggagacac tttaattaaa gatggtgttc caaaagacta
    10861 taaggttgct gataaaagtg gtcaagcaat aacatatgct tctagaaatg atgttgcttt
    10921 tgtttatcct aagggccaat ctgaacctat tgttttagtc atttttacga ataaagacaa
    10981 taaaagtgat aagccaaatg ataagttgat aagtgaaacc gccaagagtg taatgaagga
    11041 attttaatat tctaaatgca taataaatac tgataacatc ttatagtttg tattatattt
    11101 tctattatcg ttgacatgta taattttgat atcaaaacct gattttccct ttattatttt
    11161 cgagatttat tttcttaatt ctctttaaca aactagaaat attgtatata caaaaaatca
    11221 taaataatag atgaatagtt taattatagg tgttcatcaa tcgaaaaagc aacgtatctt
    11281 atttaaagtg cgttgctttt ttctcattta taaggttaaa taattctcat atatcaagca
    11341 aagtgacata attgttgaat ttatagtaac tgaaacgtat gattagggtg tatattaatt
    11401 atgagatgga aatcaatgat tgttggctat gcgagagtat catcgaacga tcaaaattta
    11461 gaaagacaac ttgaaaattt aaagacgttt tgtgctgaaa aaatatttac agaaaaacag
    11521 tctgggaaat ctattgaaaa tagaccagta cttcaagaag cacttaactt tttgaggatg
    11581 ggagatcggt ttgtagtaga atccattgat tgtttaggtc gtaattatga tgaagtgatt
    11641 cataccgtta attatttaaa agacaaagaa gtacaattaa tgattactag cttacctatg
    11701 atgaatgaag tggttggcaa tccattactg gataagttta tgaaagattt aattattcaa
    11761 atattagcaa tggtttcaga acaagagaga aatgaaagta aacgtcgaca agcgcaagga
    11821 attcaagttg cgaaggaaaa aggcgtatat aaagggcgcc ccttacttta ttcaccaaat
    11881 gctaaagatc cacagaaacg tattatttat catcgcgttg tagaaatgtt agaagaaggc
    11941 caatcaatta gtaaaattgc gaaagaagta aatataacaa ggcagacgat ttacagaatt
    12001 aaaaacgata atgatttgat ttaataaact ttgtttcaat gcaactgaga gcttctcaca
    12061 gcgtctcaaa acagatgatt ataatctatt atgagttttt aataataaga caagaaaaga
    12121 gagcgtaaat atgctctctc tttttctcat taacgaatgt ttataacgca atcatgatga
    12181 gagtattaat acagttaatt atttgaaata ctttagttta tataatgttg aattataggt
    12241 agtgaaattg tatttattat acattgtcat atcgttgttt aatgatagtt aatttaagga
    12301 taatagaatt aatattatat atagatgaac aaaacaagca acagagcaga aaaaacactt
    12361 ttagattatt tttactcatt ctctatttta tttatttttg ctataataaa taaagaatat
    12421 taaatggagg tgtttttatg aaaaagaaat ttgtttctag ttgtattgct agtacaatct
    12481 tattcggcac tttattaggt gtaacttata aagctgaagc tgcaacagta catgtagctg
    12541 gtggtgtttg gagccatggt attggaaaac attacgtatg gtcttattat agtcacaata
    12601 aaagaaatca tggttcaaca gcagtaggga aatattcatc ttttagtggt gttgctcgac
    12661 ctggtgttca atctaaggca tctgctccaa aggcttgggg tggaaacaaa acattttata
    12721 gtcttcacta attacatata ttaattaagg agaatagaca gaattaagta aacttaattc
    12781 tgtctattct cttgaaagag gcaatatgaa aaaattcaaa ttatttttag attttagtac
    12841 tttattatta atttcagggc tattattttt atttttcttc aaagaaaatg aagaaattat
    12901 acctgaaagt tccaatattt taacaatctc taattgggat aaatctaaca gtaaatcaaa
    12961 agtgttagat gtcattgaat caggcgccaa aaatcaaaat atacaaataa ttaaatccgt
    13021 taaagatttt gataataaaa aagaattttt tgttttcaac tcaaaacgaa ataattctga
    13081 ctttatacgt aataaaacat cacttttaac tccatcagat ttattaaaca gagagataaa
    13141 aggaaagtat tatattattg gtgaacattt caatgtagaa gagcttacga gtgaattaaa
    13201 aaaagctggt ttaacatctg aagttgaata cataaataga aacatattat tttttgaatt
    13261 agtcatagat aatgatttat tagtgccttt catattctta ggcgtattat atcttttgta
    13321 cttcttatat gacagaggat ataatttgaa attttatgct attcaaaagc tacatggctt
    13381 ttcaactaca cgcattattt ttcataatat tcatataaaa atcatttatt ggctagtact
    13441 taccgctatt ttctttattg ctaatatatt aatcattcat atagccaatt tagaattgga
    13501 tttcttaatg tttattcgta gatttatcgt gttgcttttt gtttttgctt gtattactac
    13561 attgctgtgg ctgatttcgt atagtcttct tttgaagtta agtattccat taatcttaaa
    13621 agggaaaaaa ggatataagt tttcaatatt tatgggtacc ttcaccaaat gtatcatggt
    13681 gctaatacta tcttttttac tggcacaaaa tttaaatgct tatacgaagt taaagaatat
    13741 atatgactct gaaaaaatct ggcaaaagtt agataattac tatacattag agatttctcc
    13801 taatattaga aattcagaag aaaaacaaat tttagaaact aatatataca atttaattaa
    13861 gaaaagcgaa caacttggtg gattgctttt aaagaataat aatatagcca atcccgataa
    13921 aaacaattac attcctgaaa atggcaatgt cttttttatc aataacaatt ttctgaattt
    13981 ctataaaaat ataaatcatg attttaaaat gattgacaat cattcggata aaataaacgt
    14041 gtttattcct ccaaggttac aatatcaaaa atctgaaatt caaaaaaatc accaaggatg
    14101 gattgacttt caaaagcaac aaaacaaaaa agtagatgtg caagtactgt caaaaaacgt
    14161 atctgttttt tcttttgatc gcacaactaa tttgaaattt gggtatctag attctccaat
    14221 agtaatgttg ttgacacttg acgatttaac aggagatttt tatttagctg ctgtttctca
    14281 aggtggatat ctatttaaag atttagatac acttaaacag ttgcttaaag ataatcacct
    14341 agaagaagaa attagcggta taactaatta caaggattct gtgttaaatg aattaaatga
    14401 aacgaaaaca aaaatgataa taacaatagt tactattatt attaacgttt tcattctttt
    14461 gatagctact gtatttgaaa caattcaata ttttagctgg aataaaaagc aactattatt
    14521 aagaaaaatt catggttatt cattattcag cagtaatgta cgttatttaa caatttctat
    14581 cttactttct attatgttag cttacactac gcatatttta tttggttcaa agatattact
    14641 attcataata atgtctattg ctattattca acatttactt caaattgctt atattaaata
    14701 tcttgaaaaa tactttaaag atttaatgag ggagatttaa acaatgaaaa gaactttaat
    14761 tattgtaaca attgtactat taatcatcat acttagtctg ttattggtta gaaatgagca
    14821 tttagatcgt tttaacccac tgttaaaaga aaaagtatct tatgccaaag ttccaaaaga
    14881 tacccaggac tatagaaata taactgtata ctctaaaaat ggagaaaaaa agagttataa
    14941 attggatttt ttaggttatg atcctacaaa agaatatgtg aaagtaaatc ataaagggaa
    15001 atatgtgcgt tcgatagaat acattacaaa agatattcct agctttttaa aatgaggtga
    15061 ccgatagtga taaaacttga aaatgttttt gtaaaaaaag gtaataaaaa cattttagac
    15121 ggatgtaact ttaattttga aaaaggaaaa tcatatgctt tagttggaga aagtggcgct
    15181 ggaaaatcta ctttacttaa cataatcgct gggttcgaag atgtatcaca aggaagcata
    15241 tatattgaag ataagttact aaaaaagaaa gtcgattttt atagatatac gttagggtat
    15301 ttgtttcaaa attttgcttt acttgaaaat caaacaatca gtcaaaattt agatttagcc
    15361 ctaaagttca aaaagaataa aaaagataat atgaacttaa agaaagaagt attaaaaaag
    15421 gtcggcctag atttagatat caagagaatt gttagttctc taagcggtgg tgaacaacaa
    15481 agagtagcat tggcaagatt aatattaaaa gatccgaaaa tcattttagc tgatgaacct
    15541 actgggtcac tagatacgaa aaatggaaaa atagttattg atttattatt gaagctactt
    15601 gatgaaaata aaactatgat tgtcgtaacg catgatttag agcttgcgaa acgatttgat
    15661 gtcatagtca atatttctga gcttagaagt taagaaatat tgataggtat agccatacat
    15721 agagaaaaaa caatatgaaa tatgattagt cattactttt gagtttttta atttaaggac
    15781 tggattttcc agtccttttt tatgatgaca aaaaacaagc ctcgcagagc acacgttttt
    15841 aatgacttat ttggttttta ataagtctag tatgttaaaa cttttctgtg tttaggtgtt
    15901 ataatcatat gcagtggaag ttgatttatt atgtcttatt caattattcg agttgttaaa
    15961 gtgaaatcaa aaacaaatac aagaggtatt caaagacata ttcaaagaga aaataaaaat
    16021 tatgaaaata tagatattga tttaagtaag tcatatttaa actatgattt agtcaatgat
    16081 actaagtttg attttaataa aaaaattgat gagaaaatcg aaaaaaatta taaagggaaa
    16141 cgcaaaatta gaacagatgc cattaaacat attgatggat taattacatc agataatgtg
    16201 ttttttaatc aattatctga agaagagaca aaatagtttt ttaagcatgc taaagcgttc
    16261 ttggaagaag aatacggtaa agaaaatctt ttatatgcga cagtacatat ggatgagata
    16321 acaccacata tgcattatgg cgtagttcct attactaaag atggacgttt aagtgcgaaa
    16381 gaagttgtag gtaataaaaa agcattaacc gaattccaag atagattcaa tacgtatatt
    16441 aataagcaag gctatgattt aaaacgagga atttctagac aacttacaaa agaaaaacat
    16501 gatcaagtta gccgttataa acaaaaaaca gaatatcata aacaaatgca tatgcgctag
    16561 aagcaaatag aggattattt aaagcaagaa aatgataaat tgatacaaaa ctaccaaaaa
    16621 tcattagata cgcttaaaaa gcttctgagt gtcatgtatg aaattaaaac taaaaatgta
    16681 ggtggatggt ttcataaaga gaagcaagaa acaggcaata ttgtaattac taaaactgat
    16741 tttgaaaaat acactaaaca aattaaagca gcacagatga tattagatga ttatgaatgt
    16801 attaaaagtg ggaagtcatt aaaaaaagca gagaaacaaa atgaaagttt agttaatgaa
    16861 ttgactagtg tccatatgga aaatgaaaag ctggttgaag aatttaatga tttagcacaa
    16921 cgttataatt atttgttgtc agagaatgaa aagaaagata aagaactaaa ttatacgctt
    16981 aagttattca atcaagtttt taaaatcatt aaaagtatga tgaaagaaga gcgttatcat
    17041 acactaataa atcatattga taatcattta gacaattcta aaatacgtga agtaatgacg
    17101 atagataaca atgatgaaca gttctttaag aagaaatatc aagcacaaga acgagaaatc
    17161 atttttaaag aagatagaga agatggttat acattataaa aatagatatt taaatgcatt
    17221 tgtttgaggt taattatttt ttaggatgaa aaataggtag cccccctcga actttttaat
    17281 atatttttat aaatcttttt aaaaaccttt ttaggggctt gttcagccct agagcaacaa
    17341 gggatttcaa tgcctaaagg agtgtttttg ccacataaaa ggagtgtttt tgccacataa
    17401 aaggagtgtt tttgccacat aaaaggagtg tttttgccac ataaaaggag tgttttaaaa
    17461 agttgtactc cttttatggt ttgacattga taataaagta gtttattgtg aataaaagtt
    17521 ttcaaatggg agtgtatatt caaacgatac tcctttaggt tcgtaataaa gggggctgta
    17581 tcatgacagg agagacagtt gtatataaaa atgaaatgaa tttagttcca ttaaggcgat
    17641 ttacagctac tgaaattaat ttattttttg cgatgtgtaa taaattaaaa gagcaagata
    17701 ctaatacatt acgtttgtct tttgatgaat taaaaaaatt aagtaattat agtccagaaa
    17761 cacgtaatat taatagattt gctaatgatt tagataatgt gtataaaaag atgttgaatt
    17821 taaccattag atatgaagat gatgatgtaa tagaaagatt cgttttattc aatcattata
    17881 gaatccataa gcgagaacaa tatctagaaa tatcaacttc ttcaaattta aaacacatat
    17941 tgaattcaat tactaataat tttacaaaat ttgaattaaa agaaatgact aggcttaaat
    18001 ctacatattc taaaaatatg tttagattac ttaaacaata caaacataca ggctatttaa
    18061 aaattcacat cgatgatttt aaaaatcgtt tagatatacc taaatcttat cgtatgactg
    18121 atattaacaa aaatgttttt aaacctatta ttattgaatt aggttctata tttaataatc
    18181 ttactatcaa taaaatcaaa gcgaaaaaag ggcgtaaaat tgaatggata gagttcacat
    18241 ttgacgctga gaaacgcatt cataacaaac gacaacctca aatgtccaag atagataaat
    18301 caagacaata tgtgaggcgt gaaaagacgc ctaaatggtt agaagaacgg tcatatgaaa
    18361 agcaacctca aaaagattac gacccacaat tagagaaaga acgagaggat tttttaaaac
    18421 aacttgaact gaattgggaa taacgcaact tttcgttgcg agttggttaa ctaaaagtat
    18481 cgtgactttt caattatttt aattatagtt tgagttaagg agtgatttta tgaaattagc
    18541 tgaagctata aaagaacaga gagaattaaa aggatggtct caagaggaat tagccaatat
    18601 attaaaggtt tccagacaaa gtgtttctaa atgggaaagt gccaaaaact atccttcctt
    18661 agatattttg attgctatga gcgatttatt cggaatctct ttagagcatt taattaaagg
    18721 agatgcacgt tttaaaaaag ttattttaga gggaaattat agagagagta acagggtacc
    18781 ttccatagtt gattttcttt atgatttttg gtggcttttc tttcctgttg ccggattttt
    18841 agtttgggcc attcattcgt tcatgggttg attttaaaat caaaatttaa tgcacatcag
    18901 gcaatatgaa aaaggaaagt atttttacga caccttgaaa tgtattgata agaatagaag
    18961 agtttacaaa aggcaatatg aaatatatac taaaagtaac aagaatttcg tttgtaaacg
    19021 cacaaaaccc cgaactattc gccgtagttc ggggttttgt ggtgaattgg ctttaatcgt
    19081 ctatattatt ttttatcacg tttatggagc taataaccaa ataacgtaac gagacaacca
    19141 ctgattgccg ttgtcatgat gtgaacaaga atttcattca taaatcgcac ctcctctcta
    19201 cgccgaattg acatctgaga gatagacgac cttcacatta taacaaatct ctccttccat
    19261 gaaataacat gaatattaat aaatgttgtt atatattaaa gataaataaa tataagggtg
    19321 ggattgataa tgtttggcga tcaaagacaa gaagctacaa aatatgtcat taaagaaggt
    19381 tatcaagata tttatttcct caacaaaaac ggggaatggt attatttcga agtaagaagt
    19441 gtctggcgtg gcaaacatat tattagagtt aaagatggtt tgttaggttg gagaaaagaa
    19501 atagtaactg aataaagata caaaatactt gccaatgcaa aagaatgaaa acgcgtcaaa
    19561 tcaacgatgt ataaaagatg cataaaagat aatgaaagta aaaagccata aatgccgtca
    19621 tataggcatt tatggctttt tgtttggtta acataatcga tgattatcag aagttcaatt
    19681 cacataaaaa agcacggcta atttatgaac ttgtcgtacc tttttaatta aaattagtta
    19741 ccaaatagac gactccaaaa ccccttttta ggttccgttt cttcactctt ttcttctata
    19801 tcactatcat tgtcatttgg attagctgaa acgtcttctc tttgtgcttc ttcaccttgt
    19861 gtactttcgt cgctttgact tccagatata tccttatatt gaatctctgt ttctttagtt
    19921 tcttcctgat ctcggttaat atttacagga ctagtagtat atgttttttc ttgtgcatca
    19981 acgttttgtc tagcgttagt agatgtatca aagggataat taaggtgttt ttctttttct
    20041 aattgatttt ctaatttttg aatcttttta ttactctcta atgccagaac ttgctgattc
    20101 tcaagcaatt ttgatctttc ttgcaactct tttctgtaaa attcaattgt tgtttcatga
    20161 cgactttctt gtttttcaat ttgattattt aaagtattaa tctgattttt aagtatttct
    20221 actatttgag catcgctttt agattcaaaa ttaatattat ccgactcttt tttttgtttg
    20281 tcttcagtag cactttcaaa accatatttc tttttatttt catttactct ttgaatgatt
    20341 ttttctatat cggtatcttc tttaataaac gaagtgtttt cctgttttat tgtctctata
    20401 ttcaatctct taatattatt aaaaatcgtt tgcttactaa cccctaattc ttcactcaac
    20461 ctttttacac ttttcatatc atcaaccctt tataaaatgt ttataaaccc ttagtaaaat
    20521 attataagct tataacgatt aataataaag taatcgacaa ataaaaatat aaattcgcca
    20581 acctttttat caagggtttg taaagggttt atggtgttta tcaaaggttt ataaacctat
    20641 attttccctt ttttaagttt attcaaagtg ctataataac cttaatataa caaaaacccc
    20701 aactactggt aatagtcggg gt
//
